Derivation of hepato-pancreatic intermediate progenitor cells from a clonal mesenchymal stem cell line of rat bone marrow origin

  • Authors:
    • Takuro Masaka
    • Masahiro Miyazaki
    • Gang Du
    • Marhaen Hardjo
    • Masakiyo Sakaguchi
    • Mikiro Takaishi
    • Ken Kataoka
    • Kazuhide Yamamoto
    • Nam-Ho Huh
  • View Affiliations

  • Published online on: October 1, 2008     https://doi.org/10.3892/ijmm_00000041
  • Pages: 447-452
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Abstract

We have recently established a clonal mesenchymal stem cell line (rBM25/S3) from adult rat bone marrow. The cells have practically unlimited proliferation capacity (over 300 PDL), maintaining multipotency for differentiation. In the present study, we examined the potential for rBM25/S3 cells to differentiate into insulin-secreting cells. When cultured in the presence of HGF and FGF-4 on Matrigel, rBM25/S3 cells expressed genes specific to pancreatic β-cells as well as those specific to hepatocytes. They still maintained proliferation capacity with a doubling time of ≈30 h. These hepato-pancreatic intermediate progenitor cells, but not the original undifferentiated rBM25/S3 cells, were induced by the overexpression of PDX-1 to produce significant amounts of insulin in a manner responding to glucose concentration in medium. The present culture system indicates a direction for further studies aimed at the realization of cell transplantation therapy for type I diabetes mellitus.

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October 2008
Volume 22 Issue 4

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Masaka T, Miyazaki M, Du G, Hardjo M, Sakaguchi M, Takaishi M, Kataoka K, Yamamoto K and Huh N: Derivation of hepato-pancreatic intermediate progenitor cells from a clonal mesenchymal stem cell line of rat bone marrow origin. Int J Mol Med 22: 447-452, 2008
APA
Masaka, T., Miyazaki, M., Du, G., Hardjo, M., Sakaguchi, M., Takaishi, M. ... Huh, N. (2008). Derivation of hepato-pancreatic intermediate progenitor cells from a clonal mesenchymal stem cell line of rat bone marrow origin. International Journal of Molecular Medicine, 22, 447-452. https://doi.org/10.3892/ijmm_00000041
MLA
Masaka, T., Miyazaki, M., Du, G., Hardjo, M., Sakaguchi, M., Takaishi, M., Kataoka, K., Yamamoto, K., Huh, N."Derivation of hepato-pancreatic intermediate progenitor cells from a clonal mesenchymal stem cell line of rat bone marrow origin". International Journal of Molecular Medicine 22.4 (2008): 447-452.
Chicago
Masaka, T., Miyazaki, M., Du, G., Hardjo, M., Sakaguchi, M., Takaishi, M., Kataoka, K., Yamamoto, K., Huh, N."Derivation of hepato-pancreatic intermediate progenitor cells from a clonal mesenchymal stem cell line of rat bone marrow origin". International Journal of Molecular Medicine 22, no. 4 (2008): 447-452. https://doi.org/10.3892/ijmm_00000041