Inhibitory effects of somatostatin on tumor necrosis factor-α-induced interleukin-6 secretion in human pancreatic periacinar myofibroblasts

  • Authors:
    • Akira Andoh
    • Kazunori Hata
    • Mitsue Shimada
    • Sanae Fujino
    • Kazuhito Tasaki
    • Shigeki Bamba
    • Yoshio Araki
    • Yoshihide Fujiyama
    • Tadao Bamba
  • View Affiliations

  • Published online on: July 1, 2002     https://doi.org/10.3892/ijmm.10.1.89
  • Pages: 89-93
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Abstract

Pancreatic periacinar myofibroblasts are considered to be therapeutic targets for the suppression of acute pancreatitis. To elucidate the mechanisms mediating the therapeutic actions of somatostatin on acute pancreatitis, we investigated how somatostatin affects the tumor necrosis factor (TNF)-α-induced interleukin (IL)-6 and IL-8 secretion from pancreatic myofibroblasts. Cytokine secretion was determined by enzyme-linked immunosorbent assay (ELISA) and Northern blotting. Nuclear factor (NF)-κB DNA-binding activity was evaluated by electrophoretic mobility shift assay (EMSAs). The expression of somatostatin receptor (SSTR) mRNA was evaluated by reverse transcription-polymerase chain reaction (RT-PCR). Somatostatin dose-dependently inhibited the TNF-α-induced IL-6 secretion. In comparison, the effects on IL-8 secretion were modest. Northern blot analysis demonstrated that somatostatin decreased the TNF-α-induced IL-6 mRNA expression, and that this effect was completely blocked by the somatostatin antagonist cyclo-somatostatin. Furthermore, somatostatin suppressed TNF-α-induced NF-κB activation. These cells bear SSTR subtypes 1 and 2. Somatostatin down-regulated the TNF-α-induced IL-6 secretion in human pancreatic periacinar myofibroblasts. These findings suggest that some of the therapeutic actions of somatostatin on acute pancreatitis might be mediated by reducing local IL-6 secretion in the pancreas.

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July 2002
Volume 10 Issue 1

Print ISSN: 1107-3756
Online ISSN:1791-244X

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Spandidos Publications style
Andoh A, Hata K, Shimada M, Fujino S, Tasaki K, Bamba S, Araki Y, Fujiyama Y and Bamba T: Inhibitory effects of somatostatin on tumor necrosis factor-α-induced interleukin-6 secretion in human pancreatic periacinar myofibroblasts. Int J Mol Med 10: 89-93, 2002
APA
Andoh, A., Hata, K., Shimada, M., Fujino, S., Tasaki, K., Bamba, S. ... Bamba, T. (2002). Inhibitory effects of somatostatin on tumor necrosis factor-α-induced interleukin-6 secretion in human pancreatic periacinar myofibroblasts. International Journal of Molecular Medicine, 10, 89-93. https://doi.org/10.3892/ijmm.10.1.89
MLA
Andoh, A., Hata, K., Shimada, M., Fujino, S., Tasaki, K., Bamba, S., Araki, Y., Fujiyama, Y., Bamba, T."Inhibitory effects of somatostatin on tumor necrosis factor-α-induced interleukin-6 secretion in human pancreatic periacinar myofibroblasts". International Journal of Molecular Medicine 10.1 (2002): 89-93.
Chicago
Andoh, A., Hata, K., Shimada, M., Fujino, S., Tasaki, K., Bamba, S., Araki, Y., Fujiyama, Y., Bamba, T."Inhibitory effects of somatostatin on tumor necrosis factor-α-induced interleukin-6 secretion in human pancreatic periacinar myofibroblasts". International Journal of Molecular Medicine 10, no. 1 (2002): 89-93. https://doi.org/10.3892/ijmm.10.1.89