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Enhancement of γ-amminobutyric acid production by Lactobacillus sakei B2–16 expressing glutamate decarboxylase from Lactobacillus plantarum ATCC 14917

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Abstract

To enhance γ-amminobutyric acid (GABA) production using genetically engineered lactic acid bacteria, glutamate decarboxylase gene was isolated from Lactobacillus plantarum ATCC 14917 and cloned into Lactobacilli-E. coli shuttle-expression vector (pTRKH2) to yield pTRKH2GAD, which was expressed in L. sakei B2-16 isolated from Kimchi. The recombinant L. sakei B2-16 showed 1.35- and 1.42-fold higher GABA production compared to the untransformed strain in 500-mL flask and 5-L fermentor scale, respectively. The maximum GABA concentration was 265.3 mM.

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References

  • Inoue K, Shirai T, Ochiai H, Kasao M, Hayakawa K, Kimura M, and Sansawa H (2003) Blood-pressure-lowering effect of a novel fermented milk containing ã-aminobutyric acid (GABA) in mild hypertensives. Eur J Clin Nutr 57, 490–495.

    Article  CAS  Google Scholar 

  • Jakobs C, Jaeken J, and Gibson K (1993) Inherited disorders of GABA metabolism. J Inherit Metab Dis 16, 704–715.

    Article  CAS  Google Scholar 

  • Jin HS, Kim JB, Yun YJ, and Lee KJ (2008) Selection of kimchi starters based on the microbial composition of kimchi and their effects. J Korean Soc Food Sci Nutr 37, 671–675.

    Article  Google Scholar 

  • Kato Y, Kato Y, Furukawa K, and Hara S (2002) Cloning and nucleotide sequence of the glutamate decarboxylaseencoding gene gadA from Aspergillus oryzae. Bioaci Biotechnol Biochem 66, 2600–2605.

    Article  CAS  Google Scholar 

  • Kim SH, Shin BH, Kim YH, Nam SW, and Jeon SJ (2007) Cloning and expression of a full-length glutamate decarboxylase gene from Lactobacillus brevis BH2. Biotechnol Bioprocess Eng 12, 707–712.

    Article  Google Scholar 

  • Komatsuzaki N, Nakamura T, Kimura T, and Shima J (2008) Characterization of glutamate decarboxylase from a high ã-amminobutyric acid (GABA)-producer, Lactobacillus paracasei. Bioaci Biotechnol Biochem 72, 278–285.

    Article  CAS  Google Scholar 

  • Kook MC, Seo MJ, Cheigh CI, Pyun YR, Cho SC, and Park H (2010) Enhanced production of ã-aminobutyric acid using rice bran extracts by Lactobacillus sakei B2-16. J Microbiol Biotechnol 20, 763–766.

    CAS  Google Scholar 

  • Manyam BV, Katz L, Hare TA, Kaniefski K, and Tremblay RD (1981) Isoniazid-induced elevation of cerebrospinal fluid (CSF) GABA levels and effects on chorea in Huntington’s disease. Ann Neurol 10, 35–37.

    Article  CAS  Google Scholar 

  • Nomura M, Nakajima I, Fujita Y, Kobayashi M, Kimoto H, Suzuki I, and Aso H (1999) Lactococcus lactis contains only one glutamate decarboxylase gene. Microbiol 145, 1375–1380.

    Article  CAS  Google Scholar 

  • O’Sullivan DJ and Klaenhammer TR (1993) High- and lowcopy-number Lactococcus shuttle cloning vectors with features for clone screening. Gene 137, 227–231.

    Article  Google Scholar 

  • Park KB, Ji GE, Park MS, and Oh SH (2005) Expression of rice glutamate decarboxylase in Bifidobacterium longum enhances ã-aminobutyric acid production. Biotechnol lett 27, 1681–1684.

    Article  CAS  Google Scholar 

  • Park KB and Oh SH (2004) Cloning and expression of a fulllength glutamate decarboxylase gene from Lactobacillus plantarum. J Food Sci Nutr 9, 324–329.

    Article  CAS  Google Scholar 

  • Park KB and Oh SH (2006) Enhancement of ã-aminobutyric acid production in Chungkukjang by applying a Bacillus subtilis strain expressing glutamate decarboxylase from Lactobacillus brevis. Biotechnol lett 28, 1459–1463.

    Article  CAS  Google Scholar 

  • Sanders JW, Leehouts K, Burghoorn J, Brands R, Venema G, and Kok J (1998) A chloride-inducible acid resistance mechanism in Lactococcus lactis and its regulation. Mol Microbiol 27, 299–310.

    Article  CAS  Google Scholar 

  • Shortt C (1999) The probiotic century: historical and current perspectives. Trends in Food Sci Technol 10, 411–417.

    Article  CAS  Google Scholar 

  • Smith DK, Kassam T, Sinqh B, and Elliot JF (1992) Escherichia coli has two homologous glutamate decarboxylase genes that map to distinct loci. J Bacteriol 174, 5820–5826.

    CAS  Google Scholar 

  • Sridhar VR, Smeianov VV, and Steele JL (2006) Construction and evaluation of food-grade vectors for Lactococcus lactis using aspartate aminotransferase and α-galactosidase as selectable markers. J Appl Microbiol 101, 161–171.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Department of Marine Biotechnology, Anyang University, 417-833, Incheon, Republic of Korea

    Moo-Chang Kook

  2. R&D Division, Biovan Co. Ltd., Yonsei University, 120-749, Seoul, Republic of Korea

    Moo-Chang Kook & Yu-Ryang Pyun

  3. Department of Chemistry, Brown University, Box H, 02912-9108, Providence, RI, USA

    Myung-Ji Seo

  4. Department of Food Science and Engineering, Ewha Womans University, 120-750, Seoul, Republic of Korea

    Chan-Ick Cheigh

  5. Department of Biotechnology, Yonsei University, 120-749, Seoul, Republic of Korea

    Sang-Jae Lee & Yu-Ryang Pyun

  6. Department of Food Science, Sunmoon University, 336-708, Asan, Republic of Korea

    Hoon Park

Authors
  1. Moo-Chang Kook
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  2. Myung-Ji Seo
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  3. Chan-Ick Cheigh
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  4. Sang-Jae Lee
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  5. Yu-Ryang Pyun
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  6. Hoon Park
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Correspondence to Hoon Park.

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Kook, MC., Seo, MJ., Cheigh, CI. et al. Enhancement of γ-amminobutyric acid production by Lactobacillus sakei B2–16 expressing glutamate decarboxylase from Lactobacillus plantarum ATCC 14917. J. Korean Soc. Appl. Biol. Chem. 53, 816–820 (2010). https://doi.org/10.3839/jksabc.2010.123

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  • DOI: https://doi.org/10.3839/jksabc.2010.123

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