Abstract
The industrial use of bovine and porcine collagen has been expanded to include foods, cosmetics, and biomaterials. However, due to outbreaks of bovine spongiform encephalopathy and foot and mouth disease, new, alternative, safe sources of collagen are being sought. In this study, pepsinsolubilized collagen (PSC) was isolated from the tissue of a starfish (Asterias amurensis) and shown to consist of (α1)2α2 heterotrimer, which is similar to calf skin type I collagen. However, the PSC was denaturated at 24.7oC, which is about 12oC lower than mammalian collagen. Immunoblotting assay using polyclonal anti-type I collagen antibody revealed that the starfish collagen contained similar affinity motifs. In XTT assay, PSC suspension had cell growth activity and no showed no cytotoxicity.
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Abbreviations
- BCIP/NBT:
-
5-bromo, 4-chloro, 3-indolylphosphate/nitro blue tetrazolium chloride
- bFGF:
-
basic fibroblast growth factor
- DMEM/F12:
-
Dulbecco’s modified eagle medium: nutrient mixture F-12
- EGF:
-
epidermal growth factor
- ELISA:
-
Enzyme-linked immunosorbent assay
- FBS:
-
fetal bovine serum
- HDF:
-
human dermal fibroblast
- PMS:
-
N-methyl dibenzopyrazine methyl sulfate
- PSC:
-
pepsin solubilized collagen
- SDS-PAGE:
-
SDS-polyacrylamide gel electrophoresis
- XTT:
-
sodium 3′-[1-(phenylaminocarbonyl)-3,4-tetrazolium]-bis(4-methoxy-6-nitro)benzene sulfonic acid hydrate
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Lee, Kj., Park, H.Y., Kim, Y.K. et al. Biochemical characterization of collagen from the starfish Asterias amurensis . J. Korean Soc. Appl. Biol. Chem. 52, 221–226 (2009). https://doi.org/10.3839/jksabc.2009.040
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DOI: https://doi.org/10.3839/jksabc.2009.040