Abstract
We investigated suitability of media and the types and concentrations of growth regulators necessary for establishing bioreactor cultures of mountain-grown ginseng. Effects of cytokinin and auxin on root growth differed with culture and accession. Low levels of cytokinin and high levels of auxin proved to be superior root-growth regulators. SH (Sheck & Hildebrant) culture medium produced better growth than blends of MS (Murashige & Skoog) and WPM (Wood plant medium) media. The best suspension culture for induction of roots was SH medium supplemented with 4 mg/L indole-3-butyric acid (IBA). Root growth under 1700 lux illuminance was reduced by as much as 10% relative to that under 3500 lux.
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Abbreviations
- IBA:
-
indole-3-butyric acid
- MS:
-
Murashige & Skoog
- NAA:
-
α-naphthaleneacetic acid
- SH:
-
Sheck & Hildebrant
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Lee, J.G., Seong, E.S., Goh, E.J. et al. Factors involved in masspropagation of ginseng (Panax ginseng C. A. Meyer) using bioreactor system. J. Korean Soc. Appl. Biol. Chem. 52, 466–471 (2009). https://doi.org/10.3839/jksabc.2009.080
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DOI: https://doi.org/10.3839/jksabc.2009.080