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Immunology and Infection

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Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems
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Efficient Recombinant Parvovirus Production with the Help of Adenovirus-derived Systems

Article DOI: 10.3791/3518-v • 13:47 min • April 23rd, 2012
April 23rd, 2012 •
*1,2, *1, 1,2, 1,2, 1,2
1Tumour Virology Division F010, German Cancer Research Center (DKFZ), 2Inserm Unit 701, German Cancer Research Center (DKFZ)
* These authors contributed equally

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Summary
Automatic Translation

Here we describe a protocol based only on cell infection, which improves the efficiency of recombinant parvovirus production by more than 100 fold in comparison to other protocols in use. This protocol relies on the use of a novel adenovirus 5-based helper containing the parvovirus VP transcription unit (Ad-VP).

Tags

Recombinant Parvovirus Adenovirus-derived Systems Rodent Parvoviruses PV Genome Promoters P4 And P38 Non-structural Proteins (NS1 And NS2) Capsid Proteins (VP1 And VP2) Anticancer Agents Oncolytic Abilities Oncosuppressive Abilities NS1 Cytotoxicity Therapeutic Transgene RecPVs Vector Viral DNA Amplification Packaging Producer Cells (HEK293T) PCMV-VP Vector Replication Defective Oncotoxic Activities Anti-cancer Agents
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