Review of the genus Calonola László, Ronkay & Witt, 2010 with description of a new species (Lepidoptera, Nolidae, Nolinae)

A new species of the Nolini genus Calonola László, Ronkay & Witt, 2010, Calonola theresiae sp. n., is described. A revised check list of the taxa of the genus is given. With 8 colour and 9 black and white figures.


Introduction
The genus Calonola was established by László, Ronkay & Witt (2010) for the conspicuous and rather detached species, Zia ectrocta Hampson, 1907, which had been treated earlier as member of the genus Aquita Walker, 1863 as Zia Walker, 1863 is a mere synonym of Aquita. Due to the similar external habitus and the genital morphology, Pisara argyria Hampson, 1894 has also been placed into Calonola by László et al. in the same article (2010). Later, a further species, Barnanola orbiculata László, Ronkay & Witt, 2010 has been transferred to the genus (László et al. 2013).
During the identification of the Nolinae material of the Hungarian Natural History Museum and the private collections of the senior and second authors, three female specimens from Central Vietnam, 5 males and 2 females from South Laos, and a female from North Thailand reminding to C. argyria were found. The examination of the copulatory organ of this taxon revealed that their distinctive external habitus is combined with significant differences in genital morphology comparing with those of C. argyria, C. ectrocta and C. orbiculata. Thus, the specimens from Vietnam, Laos and Thailand represent the fourth known species of the genus which is described in the present paper as new to science.

Material and Methods
The genital apparatuses were dissected, stained with Eosin red and mounted in Euparal on microscope slides applying standard methods of preparation (Lafontaine & Mikkola 1987). Photos of adults were taken using a Nikon D90 camera equipped with Nikkor AF Micro 60 mm lens. The genitalia dissections and mountings have been carried out using Nikon SMZ745 and Wild M7A stereomicroscopes. The genitalia photographs have been taken using a Nikon Eclipse 80i compound microscope connected to a Nikon DS-Fi1 digital camera, by courtesy of Martin Lödl and Sabine Gaal-Haszler (NHMW).

Species content of the genus with updated information on distribution:
Calonola ectrocta (Hampson, 1907)

Diagnosis.
The external appearance of Calonola theresiae sp. n. is most similar to that of C. argyria (Hampson, 1894) but the new species is easily distinguishable from its sister species by the following characters: C. theresiae has neutral white head, thorax and forewing, without intense silvery sheen which is typical of C. argyria, the postmedial line is more perpendicular to inner margin and its upper third is stronger curved inwards to large dark costal patch, the lower section of marginal field is more greyish-brown shaded, with more angulate subterminal line, the distance between postmedial and subterminal line is remarkably larger in the tornal area than in C. argyria where these two lines run very close and parallel towards inner margin; last but not least, the hindwing of the new species is considerably darker than that of C. argyria. In the male genitalia, the new species differs clearly from C. argyria by its considerably shorter and slimmer uncus, somewhat shorter, medially broader valva, conspicuously shorter harpe and much broader vinculum without a long, narrow medial process which is characteristic for C. argyria; in addition, the new species has somewhat shorter and broader aedeagus comparing with that of C. argyria with the vesica bearing a much smaller, shorter, rather thumbtack-like cornutus, whereas the cornutus of C. argyria is much longer, more robust, thorn-like.  The differences between the two species are also well expressed in the configuration of the female genitalia, as the new species has significantly, in comparison with C. argyria, longer and somewhat narrower, membranous ductus bursae (that is sclerotized in its distal part in C. argyria with a distinct lateral protrusion), remarkably smaller corpus bursae and considerably larger pair of elongate signum bursae. (Figs 3, 4). Forewing length of males 9-9.5 mm, that of females 10-10.5 mm. Head relatively large, palpi well developed; frons and vertex neutral white; compound eyes moderately large, globular; male antennae sparsely fasciculate, that of females filiform. Sexual dimorphism expressed only by the slightly different sizes of males and females. Thorax uniformly neutral white; abdomen white, ringed dorsally with brown-grey or plumbeous grey; last two abdominal segments almost entirely brownish-greyish. Forewing moderately long and broad, apically rounded, costal margin slightly convex in its apical third, outer margin gently arcuate, ventral margin nearly straight. Forewing ground colour dull-white, with short, elliptical blackish costal basal spot, large subtriangular/semilunar dark brown costal patch; marginal area suffused with somewhat greyish shaded brown except at termen. Subbasal and basal lines deleted; antemedial line very thin, blackish-brown, medially sharply angled, its ventral section weakly, medial angle and costal section sharply defined. Median area with sparse brown irroration; orbicular stigma small, rounded, blackish brown; claviform stigma cuneate black; median fascia obsolete, shadow-like, represented by a few darker brown scales; postmedial line sharply defined, blackish-brown, only slightly wavy, and rather perpendicular to inner margin but curved inwards at vein m2 and between the cubital veins. Apical third of costa with three narrow dark grey patches, apex creamy white; subterminal line wide, diffuse, dark brown followed by whitish at outer side, upper section more indistinct, lower two-thirds stronger and wavy, running rather far from postmedial line at tornus. Terminal line fine, continuous, dark brown; cilia very long, brown, with fine white basal line, and chequered with darker brown squares. Hindwing pale brown basally, gradually darkened towards apex, apex dark brown; cilia pale brown. Male genitalia (Fig. 11). Uncus rather long and narrow, apical third heavily sclerotized, relatively short, slightly arched, gradually tapered, apically pointed, beak-like; subscaphium narrow, elongate, membranous with gently arcuate moderately sclerotized lateral margins; tegumen short and narrow; transtillae very thin, pointed stick-like; fultura inferior (juxta) short and narrow, shield-like; valva medium long, medially slightly dilated, ventral margin sclerotized, evenly arcuate, costal margin membranous, basally moderately convex, then very slightly concave, apically rounded; harpe bifid with a short, thin, straight, pointed anterior and longer and thicker, apically upcurved, pointed, rather claw-like posterior process; sacculus short and narrow without processes; vinculum rather wide, relatively long, U-shaped, medially with a fine, short triangular protrusion. Aedeagus very short, rather broad, elongate quadrangular with ostium ductus ejaculatorii in its middle section; vesica armed with a single, very small, basally broadly rounded, apically finely pointed, thumbtack-like cornutus. Female genitalia (Figs 13,14). Ovipositor very short and broad, quadrangular, apophyses posteriores short, thin; 8 th tergite very short, belt-like, apophyses anteriores moderately long, thin, apically pointed. Ostium bursae (antrum) relatively broad, cup-shaped, strongly sclerotized; ductus bursae rather long and medium broad, tubular, membranous, without protrusion; cervix bursae simple, membranous; tubular distal part of corpus bursae long, gradually dilated proximally; proximal part of corpus bursae relatively small, ellipticalovoid, with a pair of long, parallel arranged, medially dilated, ribbon-like and scobinate signum bursae.

Distribution.
The specimens of the type series were collected in three countries in Indochina: in the Central Highlands of Southern Vietnam, on the Lam Vien Plateau near Da Lat town, in Champasak province, near Pakse town in Laos and in Chiang Mai province near Doi Suthep in North Thailand. Calonola theresiae is supposedly distributed all over the mountainous parts of Indochina occurring sympatrically at places with the three other Calonola species.

Etymology.
The new species is dedicated to the senior author's mother Terézia Bauer, who has always been supporting her son's scientific ambitions and efforts in lepidopterology.