Isolation and Characterization of Hederagenin from Hedera helix L. Extract with Antitumor Activity

Tatia, Rodica; Zalaru, Christina; Tarcomnicu, Isabela; Moldovan, Lucia; Craciunescu, Oana; Calinescu, Ioan

Rodica Tatia, Christina Zalaru, Isabela Tarcomnicu, Lucia Moldovan, Oana Craciunescu, Ioan Calinescu

Isolation and Characterization of Hederagenin from Hedera helix L. Extract with Antitumor Activity

Abstract:

Hederagenin, a saponin known for its therapeutic effect was isolated from leaves of Hedera helix. Hederagenin was obtained by repeated maceration of ground plant material in 95% (v/v) ethanol. The ethanolic extract was subjected to acid hydrolysis and purification with acetonitrile. Isolated hederagenin (IHe) was analyzed by HPLC-MS/MS and compared to standard hederagenin. In vitro cytotoxicity of IHe was tested in a culture of fibroblast cells from NCTC clone L929 cell line by MTT assay, using dioscin as positive control. For the determination of IHe antitumor effect, in vitro tests were performed in a culture of human cervix carcinoma Hep-2 cells cultivated in the presence of different concentrations of sample for 48 h. IHe was biocompatible in the range of concentrations 2-200 mg/mL, in NCTC cell culture. The compound showed cell cytotoxicity in the concentration range of 100-400 mg/mL, in Hep-2 cell culture, revealing its antitumor activity. These results demonstrated the possible use of hederagenin isolated from H. helix extract as an antitumor agent.

Keywords:
Hedera helix L.; hederagenin; HPLC-MS/MS; in vitro cytotoxicity; antitumor effect

Issue: 2019, Volume 70, Issue 4

Pages: 1157-1161

Publication date: 2019/5/15

https://doi.org/10.37358/RC.19.4.7084

Download Pdf Article

This article is published under the Creative Commons Attribution 4.0 International License

Citation Styles

Cite this article as:
TATIA, R., ZALARU, C., TARCOMNICU, I., MOLDOVAN, L., CRACIUNESCU, O., CALINESCU, I., Isolation and Characterization of Hederagenin from Hedera helix L. Extract with Antitumor Activity, Rev. Chim., 70(4), 2019, 1157-1161.

Vancouver
Tatia R, Zalaru C, Tarcomnicu I, Moldovan L, Craciunescu O, Calinescu I. Isolation and Characterization of Hederagenin from Hedera helix L. Extract with Antitumor Activity. Rev. Chim.[internet]. 2019 Apr;70(4):1157-1161. Available from: https://doi.org/10.37358/RC.19.4.7084

APA 6th edition
Tatia, R., Zalaru, C., Tarcomnicu, I., Moldovan, L., Craciunescu, O. & Calinescu, I. (2019). Isolation and Characterization of Hederagenin from Hedera helix L. Extract with Antitumor Activity. Revista de Chimie, 70(4), 1157-1161. https://doi.org/10.37358/RC.19.4.7084

Harvard
Tatia, R., Zalaru, C., Tarcomnicu, I., Moldovan, L., Craciunescu, O., Calinescu, I. (2019). 'Isolation and Characterization of Hederagenin from Hedera helix L. Extract with Antitumor Activity', Revista de Chimie, 70(4), pp. 1157-1161. https://doi.org/10.37358/RC.19.4.7084

IEEE
R. Tatia, C. Zalaru, I. Tarcomnicu, L. Moldovan, O. Craciunescu, I. Calinescu, "Isolation and Characterization of Hederagenin from Hedera helix L. Extract with Antitumor Activity". Revista de Chimie, vol. 70, no. 4, pp. 1157-1161, 2019. [online]. https://doi.org/10.37358/RC.19.4.7084

Text
Rodica Tatia, Christina Zalaru, Isabela Tarcomnicu, Lucia Moldovan, Oana Craciunescu, Ioan Calinescu,
Isolation and Characterization of Hederagenin from Hedera helix L. Extract with Antitumor Activity,
Revista de Chimie,
Volume 70, Issue 4,
2019,
Pages 1157-1161,
ISSN 2668-8212,
https://doi.org/10.37358/RC.19.4.7084.
(https://revistadechimie.ro/Articles.asp?ID=7084)
Keywords: Hedera helix L.; hederagenin; HPLC-MS/MS; in vitro cytotoxicity; antitumor effect

RIS
TY - JOUR
T1 - Isolation and Characterization of Hederagenin from Hedera helix L. Extract with Antitumor Activity
A1 - Tatia, Rodica
A2 - Zalaru, Christina
A3 - Tarcomnicu, Isabela
A4 - Moldovan, Lucia
A5 - Craciunescu, Oana
A6 - Calinescu, Ioan
JF - Revista de Chimie
JO - Rev. Chim.
PB - Revista de Chimie SRL
SN - 2668-8212
Y1 - 2019
VL - 70
IS - 4
SP - 1157
EP - 1161
UR - https://doi.org/10.37358/RC.19.4.7084
KW - Hedera helix L.
KW - hederagenin
KW - HPLC-MS/MS
KW - in vitro cytotoxicity
KW - antitumor effect
ER -

BibTex
@article{RevCh2019P1157,
author = {Tatia Rodica and Zalaru Christina and Tarcomnicu Isabela and Moldovan Lucia and Craciunescu Oana and Calinescu Ioan},
title = {Isolation and Characterization of Hederagenin from Hedera helix L. Extract with Antitumor Activity},
journal = {Revista de Chimie},
volume = {70},
number = {4},
pages = {1157-1161},
year = {2019},
issn = {2668-8212},
doi = {https://doi.org/10.37358/RC.19.4.7084},
url = {https://revistadechimie.ro/Articles.asp?ID=7084}
}

<<<< back

PUBLISHING SECTION

Author Guidelines

* Paper Drafting

* Paper Template

* Ethical Statement

* Paper Submission

Ghidul Autorului (ro)

Open Access & Archiving Policy

Search Authors

LATEST NEWS
	NUMAR OMAGIAL PETRU PONI.. detalii
	Revista de Chimie - 70 de ani de aparitie neintrerupta.. detalii
	English-Romanian Dictionary for Mechanical Engineering (download pdf)