Abstract
Supporting Institution
İstanbul Cerrahpaşa Üniversitesi Bilimsel Araştırma Projeleri Birimi
Project Number
TSA-2020-34537
References
- Bohlin AM, Olsen SN, Laursen SH, et al (2019): Lawsonia intracellularis associated equine proliferative enteropathy in Danish weanling foals. Acta Vet Scand, 61, 12.
- Caleffo T, Dahm V, Dos Santos JG, et al (2021): Occurence of Lawsoina intracellularis in horses raised in three regions of the state of Parana, Brazil. Semina: Cienc Agrar Londrina, 42, 2867-2876.
- Frazier ML (2008): Lawsonia intracellularis infection in horses: 2005-2007. J Vet Intern Med, 22, 1243-1248.
- Guimaraes CV, Palhares MS, Oliveira JS, et al (2009): Fecal shedding and serological cross-sectional study of Lawsonia intracellularis in horses in the state of Minas Gerais, Brazil. Equine Vet J, 41, 593-596.
- Hassenin ASH, Sena S, Gebhart CJ, et al (2017): Comparison of ELISA and IPMA for the detection of anti-Lawsonia antibodies in horses. Int J Adv Res Technol, 6, 17-24.
- Hossain MM, Oh Y, Cho HS (2016): Prevalence of antibody to and DNA of Lawsonia intracellularis in samples from wild animals in Korea. J Wildl Dis, 52, 803-808.
- Hwang JM, Seo MJ, Yeh JY (2017): Lawsonia intracellularis in the feces of wild rodents and stray cats captured around equine farms. BMC Vet Res, 13, 233.
- Kranenburg LC, Van Ree HEM, Calis ANM, et al (2011): The seroprevalence of Lawsonia intracellularis in horses in the Netherlands. Tijdschr Diergeneeskd, 136, 236-243.
- Loublier C, Cerri S, Grypeerdt A, et al (2020): High seroprevalence against Lawsoina intracellularis among adult horses in Belgium. J Equine Vet Sci, 95, 95.
- McGurrin MKJ, Vengust M, Arroyo LG, et al (2007): An outbreak of Lawsonia intracellularis infection in a standardbred herd in Ontario. Can Vet J, 48, 927-930.
- Oh Y, Hossain MM, Cho HS (2017): Seroprevalence and molecular detection of Lawsonia intracellularis from asymptomatic horses in Korea. Thai J Vet Med, 47, 543-549.
- Page AE, Slovis NM, Gebhart CJ, et al (2011): Serial use of serologic assays and fecal PCR assays to aid in identification of subclinical Lawsonia intracellularis infection for targeted treatment of thoroughbred foals and weanlings. J Am Vet Med, 238, 1482-1489.
- Page AE, Slovis NM, Horohov DW (2014): Lawsonia intracellularis and equine proliferative enteropathy. Vet Clin Equine, 30, 641-658.
- Page AE, Stills HF, Chander Y, et al (2011): Adaptation and validation of a bacteria-specific enzyme-linked immunosorbent assay for determination of farm-specific Lawsonia intracellularis seroprevalence in central Kentucky Thoroughbreds. Equine Vet J, 43, 25-31.
- Pusterla N, Gebhart CJ (2013): Equine proliferatibe enteropathy – a review of recent developments. Equine Vet J, 45, 403-409.
- Pusterla N, Mapes S, Rejmanek D, et al (2008): Detection of Lawsonia intracellularis by real-time PCR in the feces of free-living animals from equine farms with documented occurance of equine proliferative enteropathy. J Wildl Dis, 44, 992-998.
- Pusterla N, Wattanaphansak S, Mapes S, et al (2010): Oral infection of weanling foals with an equine isolate of Lawsonia intracellularis, agent of equine proliferative enteropathy. J Vet Intern Med, 24, 622-627.
- Steinman A, Raz KA, Blum SE, et al (2013): Demographic and environmental risk factors for exposure to Lawsonia intracellularis in horses in Israel. J Equine Vet Sci 34, 641-646.
- Wattanaphansak S, Gebhart CJ, Anderson JM, et al (2010): Development of a polymerase chain reaction assay for quantification of Lawsonia intracellularis. J Vet Diagn Invest, 22, 598-602.
Abstract
Equine proliferative enteropathy caused by Lawsonia intracellularis has been described in weanlings in many different countries including the USA, Western Europe, Israel, Korea and Brazil but not investigated in Türkiye and Eastern Europe. The objective of the study was to determine the seroprevalence and fecal shedding of L. intracellularis in weanling foals. A cross-sectional study was designed in randomly selected 97 weanling foals with or without clinical signs from 3 different provinces of Türkiye. Total protein and albumin levels in serum samples from 97 foals were measured using automated biochemistry analyser. Serum samples and fecal samples were also tested by bELISA and real-time PCR to detect L. intracellularis-specific antibodies and -DNA, respectively. Seropositivity was found to be 25.8% by bELISA. However, none of the fecal samples were positive by real-time PCR. Statistically, no significant associations were found between seropositivity and clinical signs or serum ALB/TP levels. Seropositivity indicated the exposure of weanling foal population to L. intracellularis for the first time in Türkiye. Further investigations are needed to better understand the epidemiology of the disease in the weanlings as well as adult horse populations and potential wildlife resorvoirs in the country/region.
Ethical Statement
The present study was approved by the Ethics Committee of University of Istanbul-Cerrahpasa (Report No. 2019/51).
Supporting Institution
This research was supported by Department of Scientific Research Projects, University of Istanbul-Cerrahpasa (Project No. TSA-2020-34537).
Project Number
TSA-2020-34537
Thanks
The authors are grateful to Jockey Club of Türkiye for giving permission to collect samples in breeding farms and also use of real time PCR system in Jockey Club of Türkiye Veliefendi Racetrack Equine Hospital Laboratory.
References
- Bohlin AM, Olsen SN, Laursen SH, et al (2019): Lawsonia intracellularis associated equine proliferative enteropathy in Danish weanling foals. Acta Vet Scand, 61, 12.
- Caleffo T, Dahm V, Dos Santos JG, et al (2021): Occurence of Lawsoina intracellularis in horses raised in three regions of the state of Parana, Brazil. Semina: Cienc Agrar Londrina, 42, 2867-2876.
- Frazier ML (2008): Lawsonia intracellularis infection in horses: 2005-2007. J Vet Intern Med, 22, 1243-1248.
- Guimaraes CV, Palhares MS, Oliveira JS, et al (2009): Fecal shedding and serological cross-sectional study of Lawsonia intracellularis in horses in the state of Minas Gerais, Brazil. Equine Vet J, 41, 593-596.
- Hassenin ASH, Sena S, Gebhart CJ, et al (2017): Comparison of ELISA and IPMA for the detection of anti-Lawsonia antibodies in horses. Int J Adv Res Technol, 6, 17-24.
- Hossain MM, Oh Y, Cho HS (2016): Prevalence of antibody to and DNA of Lawsonia intracellularis in samples from wild animals in Korea. J Wildl Dis, 52, 803-808.
- Hwang JM, Seo MJ, Yeh JY (2017): Lawsonia intracellularis in the feces of wild rodents and stray cats captured around equine farms. BMC Vet Res, 13, 233.
- Kranenburg LC, Van Ree HEM, Calis ANM, et al (2011): The seroprevalence of Lawsonia intracellularis in horses in the Netherlands. Tijdschr Diergeneeskd, 136, 236-243.
- Loublier C, Cerri S, Grypeerdt A, et al (2020): High seroprevalence against Lawsoina intracellularis among adult horses in Belgium. J Equine Vet Sci, 95, 95.
- McGurrin MKJ, Vengust M, Arroyo LG, et al (2007): An outbreak of Lawsonia intracellularis infection in a standardbred herd in Ontario. Can Vet J, 48, 927-930.
- Oh Y, Hossain MM, Cho HS (2017): Seroprevalence and molecular detection of Lawsonia intracellularis from asymptomatic horses in Korea. Thai J Vet Med, 47, 543-549.
- Page AE, Slovis NM, Gebhart CJ, et al (2011): Serial use of serologic assays and fecal PCR assays to aid in identification of subclinical Lawsonia intracellularis infection for targeted treatment of thoroughbred foals and weanlings. J Am Vet Med, 238, 1482-1489.
- Page AE, Slovis NM, Horohov DW (2014): Lawsonia intracellularis and equine proliferative enteropathy. Vet Clin Equine, 30, 641-658.
- Page AE, Stills HF, Chander Y, et al (2011): Adaptation and validation of a bacteria-specific enzyme-linked immunosorbent assay for determination of farm-specific Lawsonia intracellularis seroprevalence in central Kentucky Thoroughbreds. Equine Vet J, 43, 25-31.
- Pusterla N, Gebhart CJ (2013): Equine proliferatibe enteropathy – a review of recent developments. Equine Vet J, 45, 403-409.
- Pusterla N, Mapes S, Rejmanek D, et al (2008): Detection of Lawsonia intracellularis by real-time PCR in the feces of free-living animals from equine farms with documented occurance of equine proliferative enteropathy. J Wildl Dis, 44, 992-998.
- Pusterla N, Wattanaphansak S, Mapes S, et al (2010): Oral infection of weanling foals with an equine isolate of Lawsonia intracellularis, agent of equine proliferative enteropathy. J Vet Intern Med, 24, 622-627.
- Steinman A, Raz KA, Blum SE, et al (2013): Demographic and environmental risk factors for exposure to Lawsonia intracellularis in horses in Israel. J Equine Vet Sci 34, 641-646.
- Wattanaphansak S, Gebhart CJ, Anderson JM, et al (2010): Development of a polymerase chain reaction assay for quantification of Lawsonia intracellularis. J Vet Diagn Invest, 22, 598-602.
Details
| Primary Language | English |
|---|---|
| Subjects | Veterinary Microbiology |
| Journal Section | Research Article |
| Authors | |
| Project Number | TSA-2020-34537 |
| Publication Date | September 29, 2023 |
| Published in Issue | Year 2023Volume: 70 Issue: 4 |
