Structure and Physicochemical Properties of Water Treated under Nitrogen with Low-Temperature Glow Plasma

Water treated with low-temperature, low-pressure glow plasma (GP) in contact with air stimulates various microorganisms, the growth of various plants and provides healthy breeding of various animals. In this paper, we present water treated with GP under oxygen-free nitrogen. It is potentially suitable for breeding anaerobic microorganisms, and increasing the crops of plants utilizing atmospheric nitrogen. Deionized water saturated with oxygen-free nitrogen was treated for 5 to 90 min with low-temperature glow plasma (GP). That operation produced nitrogen in various exited states depending on the treatment time. These excited nitrogen molecules built aqueous clathrates around them. The number and structure of those clathrates depended on the time of the treatment with GP. In terms of mass, density, pH, conductivity, surface tension, Ultraviolet-Visible (UV-VIS), Fourier Transformation Infrared (FTIR), Raman and Electron Spin Resonance (ESR) spectra as well as Differential Scanning Calorimetry (DSC), the macrostructure of water saturated with nitrogen treated with GP strongly depended on the treatment time. Based on the entropy criterion, the macrostructure formed on 30 and 5 min treatment was the most and least organized, respectively.


Introduction
In a macrosystem, water molecules form a hydrogen-bonded network with localized and structured clusters. They can arrange into different structures of water, e.g., small ones of four molecules, which may then group into relatively stable octamers. The water molecules also develop much larger water clusters capable of interlinking and translating throughout space. Such clusters dynamically form both open low density and condensed network [1]. The clusters of water can interconvert between lower and higher structures. The presence of dissolved gases and other solutes and temperature affect the equilibrium between these structures influencing the properties of water [2].
There are several methods of physical and physicochemical modifications of the water macrostructure [3]. Among them, modifications with plasma are listed. They involve plasma generated with a variety of methods and various modes of their application. Thus, an abundant Through the deionized water a stream of nitrogen was bubbled for 15 min. Its flow rate depended on the volume of the water sample. In case of the 200 mL sample it was 10 mL/min). Nitrogen was deoxygenated by passing through an absorber filled with an alkaline solution of resorcinol. That water (200 mL) was placed in 250 mL glass bottles and free space over the liquid was additionally filled with deoxygenated nitrogen. The whole was placed in the chamber of the reactor [35] (Figure 1) and exposed to GP for 5, 15, 30, 60 and 90 min. Plasma of 38 • C was generated at 5 × 10 −3 mbar, 800 V, 50 Water 2020, 12, 1314 3 of 13 mA and 10 KHz frequency using a lamp patented by Oszczęda [36]. The produced water was stored at ambient temperature in 100 mL closed teflon containers.

pH
The measurements for all samples (both control samples and these treated with GP for 5 to 90 min) were performed at 25 °C in triplicates with a laboratory multifunction meter CP-501 (Elmetron, Zabrze, Poland) equipped with glass electrode HYDROMET, Type ERH-11.

Conductivity
Estimations were performed at 25 °C with an ELMETRON CPC-505 instrument (Zabrze, Poland) equipped in an ELMETRON EC-60 sensor. The estimations were taken in triplicates for both control samples and these treated with GP for 5 to 90 min.

Surface Tension
The surface tension was measured with the du Nouy'a ring technique using an STA-1 tensiometer (Sinterface, Berlin, Germany) equipped in a ring of the 9.55 radius. The normal speed of the ring was applied. The measurements were performed at 24 ± 0.5 °C for 1 g/L samples of 0.997 g/L density. The experiments were run in 7 replicates.

Differential Scanning Calorimetry (DSC)
Differential scanning calorimeter DSC 204F1 Phoenix (Netzsch, Germany) was used. Multipoint calibration (Hg, In, Sn, Bi, Zn, CsCl) was involved. Analyzed samples (12 mg) were hermetically sealed in alumina capsules. The capsules were then punctured and cooled down to −40 °C with the rate of 10 C/min followed with heating to 140 °C with the same rate. Characteristic temperatures and enthalpy of transitions (Tonset, Tmid, Tend and H) were determined with Proteus Analysis (Netzsch, Germany) software. Experiments were duplicated.

Water Density
Water sample (12 L) was placed in NETZSCH 6.239.2-64.5.00 DSC crucibles and their weight determined with precision of 10 −6 g at 22 °C using a Mettler-Toledo (Greifensee, Switzerland) XPR56 balance. Density (d) was calculated from Equation d = m/V where m and V are weight and volume of the sample, respectively.

FTIR-ATR (Fourier Transformation Infrared-Attenuated Total Reflectance) Spectra
The FTIR-ATR spectra of the film were recorded following the method formerly described [23]. Thus, the measurements were performed in the range of 4000-700 cm −1 at resolution of 4 cm −1 using a Mattson 3000 FT-IR (Madison, Wisconsin, USA) spectrophotometer. That instrument was equipped

pH
The measurements for all samples (both control samples and these treated with GP for 5 to 90 min) were performed at 25 • C in triplicates with a laboratory multifunction meter CP-501 (Elmetron, Zabrze, Poland) equipped with glass electrode HYDROMET, Type ERH-11.

Conductivity
Estimations were performed at 25 • C with an ELMETRON CPC-505 instrument (Zabrze, Poland) equipped in an ELMETRON EC-60 sensor. The estimations were taken in triplicates for both control samples and these treated with GP for 5 to 90 min.

Surface Tension
The surface tension was measured with the du Nouy'a ring technique using an STA-1 tensiometer (Sinterface, Berlin, Germany) equipped in a ring of the 9.55 radius. The normal speed of the ring was applied. The measurements were performed at 24 ± 0.5 • C for 1 g/L samples of 0.997 g/L density. The experiments were run in 7 replicates.

Differential Scanning Calorimetry (DSC)
Differential scanning calorimeter DSC 204F1 Phoenix (Netzsch, Germany) was used. Multipoint calibration (Hg, In, Sn, Bi, Zn, CsCl) was involved. Analyzed samples (12 mg) were hermetically sealed in alumina capsules. The capsules were then punctured and cooled down to −40 • C with the rate of 10 • C/min followed with heating to 140 • C with the same rate. Characteristic temperatures and enthalpy of transitions (T onset , T mid , T end and H) were determined with Proteus Analysis (Netzsch, Germany) software. Experiments were duplicated.

Water Density
Water sample (12 L) was placed in NETZSCH 6.239.2-64.5.00 DSC crucibles and their weight determined with precision of 10 −6 g at 22 • C using a Mettler-Toledo (Greifensee, Switzerland) XPR56 balance. Density (d) was calculated from Equation d = m/V where m and V are weight and volume of the sample, respectively. The FTIR-ATR spectra of the film were recorded following the method formerly described [23]. Thus, the measurements were performed in the range of 4000-700 cm −1 at resolution of 4 cm −1 using a Mattson 3000 FT-IR (Madison, Wisconsin, USA) spectrophotometer. That instrument was equipped with a 30SPEC 30 • reflectance adapter fitted with the MIRacle ATR accessory from PIKE Technologies Inc., Madison, Wisconsin, USA.

ESR (Electron Spin Resonance) Spectra
The spectra were recorded at room temperature employing an instrument constructed at Wroclaw Technical University. The range of the X-band (v = 9.5 GHz, λ = 3.2 cm) was employed at the 20-25 dB attenuation and 2048 s swiping time. An EPR controller software designed for taking the EPR spectra was applied. For samples saturated with nitrogen g ≈ 2.16.

UV/VIS (Ultraviolet/Visible) Absorption Spectra
The spectra were recorded with a Thermo Scientific Evolution 220 (Thermo Fischer Scientific, Waltham, MA, USA) spectrophotometer in the wavelength range of 190-1100 nm in a quartz cell of 10 mm path length as formerly described in our recent paper [24]. The instrument was set on automatic measuring mode at medium scan speed and 1.0 nm slit width.

Raman Spectra
The spectra were taken with a Perkin-Elmer MPF44A Fluorescence Spectrophotometer equipped with a xenon lamp (excitation at 360 nm) and 4 mL quartz cell following the method described in [23]. The spectra were recorded for: -Control distilled water stored in contact with the air, -Control distilled water saturated with deoxygenated nitrogen -Distilled water saturated with deoxygenated nitrogen exposed to GP for 5, 15, 30, 60 and 90 min.

Results and Discussion
Performed studies delivered clear evidence for deep changes evoked by GP in the water macrostructure. The plasma used in this study was generated by a lamp with the current of 10 kHz frequency. Nevertheless, changes in the GPTW macrostructure pointed to the intervention from the waves of GHz and possibly MHz frequency. Likely, the initial frequency was multiplied accordingly as a result of so-called electromagnetic interference [37].
In the UV-absorption spectra ( Figure 2) of control water containing dissolved air a shoulder of a main intensive band at 195 nm could be distinguished at approximately 230 nm. It resulted from 1 A 1 → 1 B 1 transition [3]. Its intensity was 0.225 a.u. That shoulder was followed by a weak (0.020 a.u.) maximum at 739 nm common in such spectrum of water [38]. Saturation of the water with nitrogen resulted in considerable decrease in absorbance of the shoulder to 0.151 a.u. leaving the intensity of the maximum at 739 nm intact. The spectrum represented solely the spectrum of water as the bands of molecular nitrogen could be observed in the region of 80-100 nm [39]. Thus, the decrease in the intensity of the shoulder could be related to decreased in the number of water molecules with lone electron pairs not engaged in the hydrogen bonding, that is, those providing the n → π* transitions.
According to the Roentgen hypothesis, in liquid water ice-like structures involving intermolecular hydrogen bonds coexisted with isolated H 2 O monomers [40]. One should keep in mind that control water, W, used in our studies contained dissolved oxygen as well as CO 2 , as evidenced by pH of that water (Table 1). That water had pH 5.95. Both these solutes produced a certain disorder in the macrostructure of the water. Saturating that water with nitrogen brought pH of that water to 6.68 showing that some residual CO 2 could still remain in the solution. However, first of all, the number Water 2020, 12, 1314 5 of 13 of molecules producing disorder of the macrostructure was essentially reduced as at 20 • C water dissolves 0.0425 g/kg oxygen and only 0.0185 g/kg nitrogen [41]. According to the Roentgen hypothesis, in liquid water ice-like structures involving intermolecular hydrogen bonds coexisted with isolated H2O monomers [40]. One should keep in mind that control water, W, used in our studies contained dissolved oxygen as well as CO2, as evidenced by pH of that water (Table 1). That water had pH 5.95. Both these solutes produced a certain disorder in the macrostructure of the water. Saturating that water with nitrogen brought pH of that water to 6.68 showing that some residual CO2 could still remain in the solution. However, first of all, the number of molecules producing disorder of the macrostructure was essentially reduced as at 20 °C water dissolves 0.0425 g/kg oxygen and only 0.0185 g/kg nitrogen [41].
WN5 and WN30 water seemed to have a particular macrostructures, as evidenced by their mass, density, conductivity and surface tension (Table 1). Both samples exhibited the highest mass and density, the lowest conductivity and the highest surface tension among all prepared GP-treated water samples. The absorbance of the shoulder at 230 nm in the spectrum of the water saturated with nitrogen, WN0, increased on treating with GP for 5 to 15 min. Thus, a disorder of the macrostructure of that water increased. Prolonged GP treatment ordered the macrostructure as it could be deduced from reduction of the absorbance of that shoulder below that in the spectrum of WN0. The 5 min GP treatment of WN0 most efficiently increased the absorbance of the shoulder but extending such  WN5 and WN30 water seemed to have a particular macrostructures, as evidenced by their mass, density, conductivity and surface tension (Table 1). Both samples exhibited the highest mass and density, the lowest conductivity and the highest surface tension among all prepared GP-treated water samples.
The absorbance of the shoulder at 230 nm in the spectrum of the water saturated with nitrogen, WN0, increased on treating with GP for 5 to 15 min. Thus, a disorder of the macrostructure of that water increased. Prolonged GP treatment ordered the macrostructure as it could be deduced from reduction of the absorbance of that shoulder below that in the spectrum of WN0. The 5 min GP treatment of WN0 most efficiently increased the absorbance of the shoulder but extending such treatment to 15 min reduced its intensity to that in the spectrum of non-treated water. In consequence, both spectra were like one another. The GP treatment for 30 min produced some essential change of the spectrum. The absorbance of that spectrum was initially quite low but at approximately 360 nm a jump of the spectral line was observed and, as a consequence, the intensity of the spectral line became much higher than the intensity of the remaining spectra ( Figure 2). The spectra of the water GP-treated for 60 and 90 min were identical and their absorbance was the lowest among all measured spectra ( Figure 2). Thus, treating WN0 with GP for 30-90 min organized its macrostructure.
FTIR spectra (Figure 3) of water in the range of 4000-1250 cm −1 consisted of two OH bands (stretching modes of the hydroxyl group) combined in one broad peak and OH (bending modes in the hydroxyl group) bands. The band situated at 3243 cm −1 is, in fact, composed of two bands reflecting asymmetric (longer wavelength) and symmetric (shorter wavelength) vibrations in the water molecule [3]. They could be recognized after the Gaussian distribution of that band. One might see that positions of all three bands were insensitive to the treatment with plasma, and the intensity of those bands subtly depended on it (see Table 2). treatment to 15 min reduced its intensity to that in the spectrum of non-treated water. In consequence, both spectra were like one another. The GP treatment for 30 min produced some essential change of the spectrum. The absorbance of that spectrum was initially quite low but at approximately 360 nm a jump of the spectral line was observed and, as a consequence, the intensity of the spectral line became much higher than the intensity of the remaining spectra ( Figure 2). The spectra of the water GP-treated for 60 and 90 min were identical and their absorbance was the lowest among all measured spectra ( Figure 2). Thus, treating WN0 with GP for 30-90 min organized its macrostructure.
FTIR spectra (Figure 3) of water in the range of 4000-1250 cm −1 consisted of two OH bands (stretching modes of the hydroxyl group) combined in one broad peak and OH (bending modes in the hydroxyl group) bands. The band situated at 3243 cm −1 is, in fact, composed of two bands reflecting asymmetric (longer wavelength) and symmetric (shorter wavelength) vibrations in the water molecule [3]. They could be recognized after the Gaussian distribution of that band. One might see that positions of all three bands were insensitive to the treatment with plasma, and the intensity of those bands subtly depended on it (see Table 2).  Saturation of the water containing dissolved air with oxygen free nitrogen increased the deal of asymmetric stretching modes of the water. It was manifested by the changes of the A/B intensity ratio. Independently of the applied GP treatment time the deal of the water molecules with asymmetrically stretched O-H bonds declined although that decline was irregular against the GP  Saturation of the water containing dissolved air with oxygen free nitrogen increased the deal of asymmetric stretching modes of the water. It was manifested by the changes of the A/B intensity ratio. Independently of the applied GP treatment time the deal of the water molecules with asymmetrically stretched O-H bonds declined although that decline was irregular against the GP treatment time. Prolonged treatment recovered, to a certain extent, asymmetrically vibrating molecules. The presence of nitrogen resulted in some, irregular against the treatment time, increase in the intensity of the B and C bands. Such behavior differed from that of the water containing dissolved air. The latter water contained the highest number of asymmetrically vibrating molecules after the 5 min GP treatment [24].
Raman spectra taken with an instrument equipped in a xenon lamp offered the most convincing evidence for the changes of the macrostructure of water induced by the treating water with GP ( Figure 4). The spectrum of control, deionized water maintained in the air, W, consisted of fairly intensive, clearly complex band centered at 365 nm followed by a shoulder and another, less intensive band at 372 nm. That band had at least two shoulders on its long wavelength side. Saturation of deionized water with oxygen-free nitrogen, WN0, essentially changed the spectral pattern. The weaker non-symmetric band at 370 nm was preceded by at least two shoulders and followed by a fairly intensive band at 377 nm. That band exhibited a weak shoulder on its long wavelength side. The treating the latter water with GP for 5 min, WN5, drastically changed the spectral pattern. A very strong complex band with shoulders on its both sides developed at 373 nm. In the region of 360-379 nm, the spectrum of the water treated with GP for 15 min, WN15, was identical with that of water treated with GP for 5 min but additional weak band appeared at 382 nm. The character of the spectrum of water treated under nitrogen for 30 min, WN30, resembled that taken for the water treated for 5 min. There was a first complex weaker band at 368 nm followed by more intensive band at 376 nm. The spectra of water treated under nitrogen for 60 (WN60) and 90 (WN90) min were considerably less intensive. They presented one complex broad band each with several shoulders. The spectrum of WN60 was more intensive than the spectrum of WN90. The maxima were centered at 375 and 371 nm, respectively. treatment time. Prolonged treatment recovered, to a certain extent, asymmetrically vibrating molecules. The presence of nitrogen resulted in some, irregular against the treatment time, increase in the intensity of the B and C bands. Such behavior differed from that of the water containing dissolved air. The latter water contained the highest number of asymmetrically vibrating molecules after the 5 min GP treatment [24].
Raman spectra taken with an instrument equipped in a xenon lamp offered the most convincing evidence for the changes of the macrostructure of water induced by the treating water with GP ( Figure 4). The spectrum of control, deionized water maintained in the air, W, consisted of fairly intensive, clearly complex band centered at 365 nm followed by a shoulder and another, less intensive band at 372 nm. That band had at least two shoulders on its long wavelength side. Saturation of deionized water with oxygen-free nitrogen, WN0, essentially changed the spectral pattern. The weaker non-symmetric band at 370 nm was preceded by at least two shoulders and followed by a fairly intensive band at 377 nm. That band exhibited a weak shoulder on its long wavelength side. The treating the latter water with GP for 5 min, WN5, drastically changed the spectral pattern. A very strong complex band with shoulders on its both sides developed at 373 nm. In the region of 360-379 nm, the spectrum of the water treated with GP for 15 min, WN15, was identical with that of water treated with GP for 5 min but additional weak band appeared at 382 nm. The character of the spectrum of water treated under nitrogen for 30 min, WN30, resembled that taken for the water treated for 5 min. There was a first complex weaker band at 368 nm followed by more intensive band at 376 nm. The spectra of water treated under nitrogen for 60 (WN60) and 90 (WN90) min were considerably less intensive. They presented one complex broad band each with several shoulders. The spectrum of WN60 was more intensive than the spectrum of WN90. The maxima were centered at 375 and 371 nm, respectively. Observed spectral changes were irregular against the GP treatment time suggesting building, for instance, aqueous clathrates of excited molecular nitrogen of varying excited states. The clathrates were formed of varying number of the guest molecules in aqueous cages built of different number of the water molecules. Such GP treatment time dependent irregularities were observed also in case of water GP-treated in the air [24]. Observed spectral changes were irregular against the GP treatment time suggesting building, for instance, aqueous clathrates of excited molecular nitrogen of varying excited states. The clathrates were formed of varying number of the guest molecules in aqueous cages built of different number of the water molecules. Such GP treatment time dependent irregularities were observed also in case of water GP-treated in the air [24].
Differential Scanning Calorimetry measurements delivered further information of the water macrostructure. The measurements were performed in the three-step regime, that is (i) freezing from room temperature to −40 • C, (ii) melting and (iii) heating to +140 • C. A typical graph is presented in Figure 5. The results are given in Table 3.
Differential Scanning Calorimetry measurements delivered further information of the water macrostructure. The measurements were performed in the three-step regime, that is (i) freezing from room temperature to −40 °C, (ii) melting and (iii) heating to +140 °C . A typical graph is presented in Figure 5. The results are given in Table 3. Replacement of oxygen and carbon dioxide dissolved in control water, W, with nitrogen, WN0, had little effect upon temperature of phase transitions; however, their enthalpy considerably changed. Results of the GP treatment of water saturated with nitrogen depended upon the treatment time in an irregular manner, particularly in case of freezing. Except for WN30 and WN60, T1onset, T1mid and T1end parameters increased by approximately 1 °C in respect to these for WN0. Generally, the treatment with GP produced a small decrease in the temperature parameters for melting and freezing but data for WN5 and WN30 spoiled that relation. Areas of the phase transitions declined irregularly against the treatment time.
Entropy calculated from Equation (1) (see Table 4) declined by the treatment in the manner correlated with specific heat, Cp.

Sample b Freezing
Melting Evaporating a Temperature is given in • C and areas in J/g. b See Table 1 for notation.

T 1 onset T 1 mid T 1 end Area 1 T 2 onset T 2 mid T 2 end Area 2 T 3 onset T 3 mid T 3 end Area
Replacement of oxygen and carbon dioxide dissolved in control water, W, with nitrogen, WN0, had little effect upon temperature of phase transitions; however, their enthalpy considerably changed. Results of the GP treatment of water saturated with nitrogen depended upon the treatment time in an irregular manner, particularly in case of freezing. Except for WN30 and WN60, T 1 onset, T 1 mid and T 1 end parameters increased by approximately 1 • C in respect to these for WN0. Generally, the treatment with GP produced a small decrease in the temperature parameters for melting and freezing but data for WN5 and WN30 spoiled that relation. Areas of the phase transitions declined irregularly against the treatment time.
Entropy calculated from Equation (1) (see Table 4) declined by the treatment in the manner correlated with specific heat, C p . Thus, the treatment with GP changed the equilibrium between tetrahedral clathrates and ordered bulk structures. Nitrogen favored ordering of the macrostructure in respect to that in control water stored in the air probably, first of all, because of decreased content of disordering macrostructure solutes.
ESR spectra of water stored in the air exhibited a signal common for the presence of unpaired spins. They were ascribed to the triplet state of oxygen dissolved in that water. The signal ceased after the treatment of the water with GP and that fact was interpreted as the result of taking the excited singlet state [24]. In contrast to this, the ESR spectrum of the water saturated with nitrogen prior to its treatment with GP (W) did not exhibit any signal. After the treatment with GP, the ESR spectra of that water revealed a signals pattern that depended on the GP treatment time. Figure 6 presents ESR spectra of deionized water saturated with nitrogen after treating with GP for 5 to 90 min. The time dependent pattern of the signals reflects a known complexity of the excitation of the nitrogen molecule [42,43]. This problem will be addressed in a separate paper. These spectra demonstrated that depending on the treatment time differently built aqueous clathrates were generated with different excited state nitrogen molecules inside what was also documented in the corresponding Raman spectra (Figure 3).
In our former papers, following paper by Ramya and Venkanathan [44], formation of aqueous clathrates with singlet oxygen [24] as the guest molecules was postulated. It was suggested that molecular orbitals of those guests promoted the formation of the clathrates themselves and controlled their composition as well as their structure. Entropy values of the water treated with GP for varying time (Table 4), thus, containing various forms of excited nitrogen molecules, declined in the following order: That order suggested the stimulation of the formation of the clathrates and their stability dependent on the excited state of the nitrogen molecule. That order roughly correlated with the order of declining absorbance of the shoulder at 230 nm in the UV absorption spectra of those waters (Figure 1), that is, with declining number of non-engaged lone electron pairs. It was equivalent to increasing ordering of the water macrostructure.  Thus, the treatment with GP changed the equilibrium between tetrahedral clathrates and ordered bulk structures. Nitrogen favored ordering of the macrostructure in respect to that in control water stored in the air probably, first of all, because of decreased content of disordering macrostructure solutes.
ESR spectra of water stored in the air exhibited a signal common for the presence of unpaired spins. They were ascribed to the triplet state of oxygen dissolved in that water. The signal ceased after the treatment of the water with GP and that fact was interpreted as the result of taking the excited singlet state [24]. In contrast to this, the ESR spectrum of the water saturated with nitrogen prior to its treatment with GP (W) did not exhibit any signal. After the treatment with GP, the ESR spectra of that water revealed a signals pattern that depended on the GP treatment time. Figure 6 presents ESR spectra of deionized water saturated with nitrogen after treating with GP for 5 to 90 min. The time dependent pattern of the signals reflects a known complexity of the excitation of the nitrogen molecule [42,43]. This problem will be addressed in a separate paper. These spectra demonstrated that depending on the treatment time differently built aqueous clathrates were generated with different excited state nitrogen molecules inside what was also documented in the corresponding Raman spectra (Figure 3).
In our former papers, following paper by Ramya and Venkanathan [44], formation of aqueous clathrates with singlet oxygen [24] as the guest molecules was postulated. It was suggested that molecular orbitals of those guests promoted the formation of the clathrates themselves and controlled their composition as well as their structure. Entropy values of the water treated with GP for varying time (Table 4), thus, containing various forms of excited nitrogen molecules, declined in the following order: That order suggested the stimulation of the formation of the clathrates and their stability dependent on the excited state of the nitrogen molecule. That order roughly correlated with the order of declining absorbance of the shoulder at 230 nm in the UV absorption spectra of those waters (Figure 1), that is, with declining number of non-engaged lone electron pairs. It was equivalent to increasing ordering of the water macrostructure.

Conclusions
Already elimination of dissolved oxygen and carbon dioxide from deionized water by blowing with the stream of nitrogen resulted in a higher ordering of the water macrostructure.
The subsequent treatment of the water with low-temperature glow plasma of low frequency (GP) produced aqueous clathrates with nitrogen guest molecules in various exited states depending on the treatment time. Based on the entropy criterion, the macrostructure formed on 30 and 5 min GP treatment is the most and least organized, respectively. The entropy of particular macrostructures was not linear against the GP treatment time. Independently of the applied GP treatment time the deal of the water molecules with asymmetrically stretched O-H bonds declined and the decline was irregular against the treatment time. In terms of such selected physicochemical properties as mass, density, pH, conductivity and surface tension saturated with nitrogen water treated with GP for 5 and 30 min distinctly differ from the samples treated for 15, 60 and 90 min.
Author Contributions: J.C. run Raman spectra, K.C. run pH, conductivity measurements, W.C. run ESR spectra, K.K. run UV-VIS and Infrared Spectra, H.K. run Raman spectra, D.K. Thermogravimetry measurements, Z.O. equipped a research team in nanowater, P.T. invented the project, coordinated study and designed the text of

Conclusions
Already elimination of dissolved oxygen and carbon dioxide from deionized water by blowing with the stream of nitrogen resulted in a higher ordering of the water macrostructure.
The subsequent treatment of the water with low-temperature glow plasma of low frequency (GP) produced aqueous clathrates with nitrogen guest molecules in various exited states depending on the treatment time. Based on the entropy criterion, the macrostructure formed on 30 and 5 min GP treatment is the most and least organized, respectively. The entropy of particular macrostructures was not linear against the GP treatment time. Independently of the applied GP treatment time the deal of the water molecules with asymmetrically stretched O-H bonds declined and the decline was irregular against the treatment time. In terms of such selected physicochemical properties as mass, density, pH, conductivity and surface tension saturated with nitrogen water treated with GP for 5 and 30 min distinctly differ from the samples treated for 15, 60 and 90 min.
Author Contributions: J.C. run Raman spectra, K.C. run pH, conductivity measurements, W.C. run ESR spectra, K.K. run UV-VIS and Infrared Spectra, H.K. run Raman spectra, D.K. Thermogravimetry measurements, Z.O. equipped a research team in nanowater, P.T. invented the project, coordinated study and designed the text of this report, M.W. run surface tension and water density. All authors jointly participated in interpretation of all data and in writing report. All authors have read and agreed to the published version of the manuscript.
Funding: This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.