KSHV Reactivation and Novel Implications of Protein Isomerization on Lytic Switch Control

In Kaposi’s sarcoma-associated herpesvirus (KSHV) oncogenesis, both latency and reactivation are hypothesized to potentiate tumor growth. The KSHV Rta protein is the lytic switch for reactivation. Rta transactivates essential genes via interactions with cofactors such as the cellular RBP-Jk and Oct-1 proteins, and the viral Mta protein. Given that robust viral reactivation would facilitate antiviral responses and culminate in host cell lysis, regulation of Rta’s expression and function is a major determinant of the latent-lytic balance and the fate of infected cells. Our lab recently showed that Rta transactivation requires the cellular peptidyl-prolyl cis/trans isomerase Pin1. Our data suggest that proline‑directed phosphorylation regulates Rta by licensing binding to Pin1. Despite Pin1’s ability to stimulate Rta transactivation, unchecked Pin1 activity inhibited virus production. Dysregulation of Pin1 is implicated in human cancers, and KSHV is the latest virus known to co-opt Pin1 function. We propose that Pin1 is a molecular timer that can regulate the balance between viral lytic gene expression and host cell lysis. Intriguing scenarios for Pin1’s underlying activities, and the potential broader significance for isomerization of Rta and reactivation, are highlighted.

currently understood how late gene synthesis is regulated by viral DNA replication itself, independently of DE gene expression. Replication-dependent epigenetic regulation, such as histone modification, is among the possibilities [7,[19][20][21]. Envelopment leads to maturation of virions, complete with decoration of envelopes with viral glycoproteins, such as K8.1 [3,4,22,23]. It is currently thought that infected B cells, the viral reservoir, release virions that can then disseminate to the lymphatic endothelium and seed for KS tumor development [2,4].

Function and Regulation of Rta Lytic Switch Protein
Replication and transcription activator (Rta) is a 691 amino acid (aa) IE transcription factor encoded from the major IE locus tricistronic transcript [2,34,35]. Its transcript is among the first produced following chemical induction by 12-O-tetradecanoyl phorbol-13-acetate (TPA), being expressed within 1 hpi [2,17,34]. This gene alone was identified to encode the lytic switch protein of KSHV, and is necessary and sufficient for the onset of productive lytic reactivation, with concomitant release of infectious virions [2,4,7,34,35]. Rta's function was confirmed via observations that ectopic Rta induced reactivation alone in infected B cells, as well as by functional binding analyses with truncation and dominant negative (DN) mutants and by genetic analyses with Rta-deficient viral bacmid-infected cells, both of which were incapable of reactivation [2,7,[35][36][37]. Further, addition of TPA could not induce the Rta-deficient virus, but induction was rescued by ectopic Rta expression [36]. Rta also autoactivates its own promoter, an activity characteristic of protein switches [7,[37][38][39].

Mechanisms of Rta-Mediated Transactivation
While Rta is both necessary and sufficient for viral reactivation, its transactivation activity is inefficient. This is underscored by reports demonstrating that Rta activity alone, in the absence of ongoing cellular or viral protein production, is unable to induce the full repertoire of lytic genes [7,16,37,46,[48][49][50][51]. Rta's inefficiency is also supported by data showing that Rta is weakly or nontransactive when mutated to prevent binding of specific cofactors, or when cofactor binding motifs in promoters are disrupted [7,16,37,39,46,47,49,[52][53][54][55]. Finally, Rta expression does not necessarily translate into productive replication in infected cells, as typically fewer than 20% of Rta-positive PEL cells coexpress true late protein K8.1 (produced only following viral replication, and often utilized as a reactivation marker) [37,50]. The implications of these findings, are twofold. First, that Rta requires viral and cellular protein interactions to guide it through the full lytic cascade, via direct binding, posttranslational modifications or both. Second, that the conserved inefficiency of Rta lytic switch function may be important for KSHV pathogenesis.
Rta's transcription at downstream gene promoters is highly complex. In broad terms, and with exceptions, specificity to target promoters can be characterized as either direct or indirect, and as independent or dependent on interaction with the Notch signaling pathway effector recombination signal binding protein (RBP-Jk, which will be discussed below) [2,4,7,[37][38][39]46,55,56]. Direct transactivation occurs by Rta binding to Rta-responsive elements (RREs) within certain promoters [4,37,38,46,[55][56][57][58]. RREs, both RBP-Jk-dependent and -independent, can vary significantly, but four general consensus sequences have so far been uncovered: the palindromic repeat TTCCAGGAT(N)TTCCTGGGA, where N represents as many as sixteen random bases; multiple units of an A/T trinucleotide repeat, found in the K-bZIP, DE gene Mta and glycoprotein-encoding gene K1 promoters; recently-identified, TATA-box proximal elements known as "CANT DNA repeats" (discussed below); and the interferon (IFN) stimulated response element (ISRE)-like motif (A/G)NGAAANNGAAACT, found in promoters for vIL-6, vGPCR and ORF8 [37,38,46,55,56]. Binding to the latter depends on partial homology of Rta's DBD to IRF family members [37,46]. Meanwhile, Rta binding affinity is largely proportional to the extent of transactivation for RBP-Jk-independent promoters [7,37]. The prototypical genes are kaposin and nut-1, with Rta binding at the nut-1 promoter with nanomolar (nM) affinity, and with nut-1 being the most abundant transcript produced during the lytic cycle [2,35,37,53,59,60]. Direct Rta transactivational targets have been identified by several labs. One such screen from our lab reported eight direct targets, including the promoters for nut-1, Mta, viral interleukin (vIL)-6, viral shutoff exonuclease (vSOX) and vOX2 [57]. Additional promoters, such as ORF45 and the miRNA locus, have also been described, though use of different methods and cell lines makes confirmation of authentic direct promoters difficult [37,38,56]. Finally,Rta also can bind DNA combinatorially with cellular or viral cofactors, such as octamer 1 (Oct1) and others, or enhance their transactivation, such as CREB binding protein (CBP); these are discussed below [4,37,39,46,52].

Rta Positively and Negatively Interacts with Host and Viral Cofactors
Rta is involved in a host of other important lytic cycle functions beyond its primary role as a transcription factor. For one, viral DNA replication is unable to proceed without Rta activity at oriLyts [7,37,61]. There, Rta binds to RREs as an origin binding protein in conjunction with K-bZIP and CCAAT/enhancer binding protein (C/EBP)-α (which itself has palindromic motifs within oriLyts) [37,55,61]. Together, these proteins recruit the core replication machinery to viral genomic DNA [7,61]. Rta also inhibits p53 transcription via a direct interaction with CBP, and modulates both IFN regulatory factor (IRF)7 and cellular K-Rta binding protein (K-RBP) stability via Rta's E3 ubiquitin ligase (Cys/His-rich) domain [62][63][64][65][66]. Rta ubiquitylates the Notch and hypoxia response pathway target protein Hey1, a transcriptional repressor that may be involved in cell differentiation; it has been further suggested, but not confirmed, that Rta can destabilize latency-associated nuclear antigen (LANA)-1 and K-bZIP [7,46,64,67,68]. Rta's ubiquitylation of some of the above proteins is in response to their own repressive function against Rta; in fact, multiple factors positively and negatively regulate Rta expression and activity for tight control over reactivation from latency.
Thus, also, do multiple proteins, beyond K-bZIP in the context of DNA replication, enhance Rta's expression and functions. For instance, Pim1 and Pim3, proto-oncoproteins involved in cell cycle and apoptotic pathways, upregulate Rta autoactivation by binding to and repressing LANA's inhibition of the Rta promoter [86]. Viral G protein coupled receptor (vGPCR) may also negate LANA inhibition by reducing HDAC activity to allow for Sp1-and Sp3-dependent Rta promoter activity [87,88]. Hypoxia inducible factor (HIF)-1α can directly activate the Rta promoter, due to the presence of several putative hypoxic response elements (HREs) [69,89,90]. Interestingly, LANA was reported to activate Rta transcription through binding to HIF-1α at HREs during hypoxia, suggesting a context-dependent function for LANA and a mechanistic explanation for hypoxia-driven reactivation [69].
It remains unclear, however, how constitutively active HIF-1α is incapable of inducing Rta expression in the absence of hypoxia. X-box binding protein 1 (XBP-1), a critical inducer of the unfolded protein response (UPR) activated in stress conditions (including hypoxia) by the endoplasmic reticulum (ER) sensor BiP (Grp78), also directly transactivates Rta at ACGT-containing elements [91,92]. As XBP-1 activity induces B cell differentiation into secretory plasma-like cells, this process may be important for viral pathogenesis [91]. The aforementioned CBP, as well as p300, are transcriptional coactivators with intrinsic histone acetyltransferase (HAT) activity that bind to Rta at downstream Rta target promoters [7,37,52]. AP-1 is a complex of c-Jun and c-Fos proteins and may contribute the strongest transactivation activity for Rta expression, as evidenced by induction of a productive replication cycle by TPA that is similar to Rta-mediated induction [2,4,7,14,79,81,93]. Signal transducer and activator of transcription 3 (STAT3), a growth factor-and cytokine-responsive regulator, is dimerized by Rta, allowing STAT3 to translocate to the nucleus and induce STAT3 transcriptional targets [94]. Rta also binds to C/EBP-α, and recruits basal transcription complex Mediator and chromatin remodeling complex SWI/SNF, at Rta promoters to potentiate viral gene transcription [4,7,37]. Oct1 is a cofactor with Rta, with binding sites in the Rta promoter stimulating Rta autoactivation, and conversely, in the LANA promoter to upregulate LANA as a negative feedback circuit [4,7,37,39]. Oct1 is also necessary for transactivation of the K-bZIP promoter [37,39]. K-bZIP itself can work cooperatively with Rta to facilitate transcription at select promoters, including for Rta, vIL-6, Mta and K-bZIP; as K-bZIP seems to both activate and repress its own promoter and have dual functions with Rta, its regulation of lytic replication is presumably complex [7,37].
Finally, Mta, in addition to its functions described above, binds to and enhances Rta transactivation of selective downstream viral genes [37,50,55,95]. Loss or mutation of Mta showed that it is required for productive replication [37,50]. It is one of the first lytic genes expressed, one of the few directly targeted by Rta, and Mta protein can activate promoters in concert with Rta, including for itself, nut-1, Rta, kaposin and viral thymidine kinase (vTK) [37,50,81]. Mta also has transactivation potential alone in some contexts, as it can activate transcription of the nut-1 promoter independently of Rta; posttranscriptional roles likely exist for certain promoters as well, such as for nut-1 and viral DNA polymerase [37,50,95,96]. Mta binds mRNA and stabilizes a variety of transcripts, and one potential model is that Mta synergizes with Rta for transcriptional initiation and then enhances elongation by binding to and stabilizing nascent transcripts, where it may remain bound to enact its downstream activities [37,95,96]. Mta's importance in productive replication was highlighted by data showing that, despite the aforementioned dearth of Rta-expressing cells positive for reactivation as indicated by K8.1 expression (fewer than 20%), more than 80% of Mta-expressing cells were reactivated [37,50]. As Mta represents a much better predictor of virus proceeding through a complete lytic cascade, it has been characterized as a "commitment factor" that drives inefficient Rta function in the direction of productive replication [37,50].

RBP-Jk Is Essential for Rta-Mediated Transactivation
Of all single Rta cofactors, however, canonical Notch pathway effector RBP-Jk (also known as CSL, for EBV core promoter-binding factor [CBF]-1/suppressor of hairless [Su(H)]/longevity assurance gene [Lag]-1) is the only one shown to be essential for Rta transactivation activity at viral and cellular promoters and for productive reactivation [4,7,37,[47][48][49]. The Notch pathway is one of the oldest evolutionarily conserved signaling pathways in multicellular organisms [97]. It is involved primarily in development and cell fate, including intercellular communication and stem cell differentiation [97]. It also regulates apoptosis and angiogenesis, and Notch pathway dysregulation, which causes self-renewal and angiogenic tumor growth, is implicated in a variety of lymphoid cancers, such as T cell leukemias [97][98][99][100]. When signals including vascular endothelial growth factor (VEGF) and other cytokines induce Notch ligands Jagged or Delta-like to interact with one of the four human Notch single-pass transmembrane receptors, cleavage events release the Notch intracellular domain (NICD), which translocates to the nucleus and binds to RBP-Jk [46,97,98,[100][101][102][103][104]. In the canonical Notch pathway, prior to NICD association, RBP-Jk is constitutively bound to promoter targets as part of HDAC corepressor complexes, at a (C/T)GTGGGAA consensus motif, and represses transcription [37,48,55,97]. NICD binding disrupts this repression, allowing it to recruit HAT proteins, signal through activated RBP-Jk and transcribe downstream Notch pathway genes, which include Hey and Hes family repressors [97,98,101,103,104].
In KSHV-infected cells induced for lytic reactivation, Rta associates with RBP-Jk in order to transactivate downstream viral and cellular genes [37,[46][47][48][49]. Many of Rta's gene promoter targets are RBP-Jk-dependent (though many also require, or are enhanced by, additional interacting proteins, some of which were described above), including Mta, K-bZIP, LANA, vGPCR, IL-6, Hes1, vTK, modulator of immune recognition (MIR)1 and MIR2, vCCL1 and others [4,16,37,38,46]. In fact, RBP-Jk binding has been identified to at least 99 sites within the KSHV genome in infected cells ( [105], and as many as 34 Rta transcriptionally-activated viral genes have been described; this suggests the potential for an Rta-RBP-Jk complex to induce the entire lytic cascade [37,106]. Proof for RBP-Jk as a cofactor in Rta-mediated transactivation required for productive replication came in the form of truncation and mutation analyses of both proteins as well as target promoters [7,16,36,37,[47][48][49]51,54]. The prototypical promoter for characterization of RBP-Jk interactions is Mta. RBP-Jk binding sites lie proximal to Rta binding elements [37,47,55]. Alterations to either of these sites reduced or prohibited Rta and/or RBP-Jk binding, transactivation or both, depending on location of a mutation within the promoter or on rearrangement between particular elements [37,47,55]. Independent binding of each protein at promoters, and subsequent ternary complex formation with promoter DNA, was required for optimal transactivation [37,47,53,55]. While RBP-Jk DNA binding was necessary for transactivation of Mta, RBP-Jk was found, unusually, not to constitutively bind to KSHV promoters in the absence of Rta, in sharp contrast to its mechanism for canonical Notch signaling [7,37,[47][48][49]. This was determined by lack of RBP-Jk enrichment on viral promoters during latency, and by evidence that a constitutively active RBP-Jk mutant fused to the TAD of HSV-1 protein VP16 (RBP-Jk/VP16) was unable to bind to promoters alone [37,47,49]. Rta DNA binding, meanwhile, was determined to not be sufficient for transactivation at some RBP-Jk-dependent promoters [37,47,51]. An Rta mutant lacking its TAD, which begins at aa 530, but with its DBD (aa 1-272) intact (RtaΔSTAD), was also unable to activate its downstream genes alone; however, when combined with RBP-Jk/VP16, RtaSTAD rescued RBP-Jk DNA-binding at the Mta promoter [37,47]. The interaction also rescued transactivation [37,47]. Meanwhile, RBP-Jk-null fibroblasts were deficient in transactivation at Mta, but not nut-1, which is a direct Rta target; ectopic expression of RBP-Jk rescued this activity [37,48,49]. Taken together, Rta binding to RBP-Jk appears to stimulate RBP-Jk DNA binding at Rta downstream promoters containing both Rta-and RBP-Jk-specific elements, and in conjunction with additional cofactors at certain promoters, activates gene transcription. While this basic model for RBP-Jk-dependent, Rta-mediated transactivation addressed many of the questions surrounding regulation of KSHV gene expression, it was still not fully understood how Rta physically bound to its promoter elements in complex with RBP-Jk. Originally, 40 nt and 26 nt sequences containing identical, 16-nt palindromic RREs were defined within the Mta and K-bZIP promoters adjacent to an RBP-Jk binding site, but as flanking mutations in the Mta promoter, including in TATA-proximal sequences, revealed profound defects to transactivation without affecting RBP-Jk binding, the architecture required for Rta binding developed into a more complex picture [7,37,[46][47][48]55]. First, it was noted that Rta elements were present upstream and downstream of the RBP-Jk binding site [7,37,[46][47][48]55]. Second, Rta bound with high affinity to A/T trinucleotide repeat units within these elements, and the number and position of elements corresponded to the strength of Rta DNA binding [7,37,[46][47][48]55]. Third, DNA footprinting mapped to four sites, distal and proximal to the RBP-Jk element, with the proximal sites flanking both sides of the element [37,55]. These four sites overlapped with A/T repeats. It was determined that the sites shared the consensus sequence ANTGTAACANT(A/T)(A/T)T, known as the "CANT DNA repeat" [37,55]. These units were repeated seven times in the four sites, two of which formed palindromes [37,55]. Further, it was shown that CANT repeats are present at a variety of Rta responsive promoters adjacent to RBP-Jk binding motifs (including at oriLyts), and represent a broadly-applicable RRE that defines Rta-RBP-Jk ternary complex formation and transcriptional mechanics [37,46,55]. Rta binds relatively weakly to single CANT DNA elements or palindromes, but binds with nM affinity to the full cohort of 7 CANT repeats in the Mta promoter.
While the motif itself still needs to be elucidated, it is clear based on functional binding studies that Rta interaction with RBP-Jk occurs within a 117 aa region of Rta between aa 414 and 530, just N-terminal to the Ser/Thr-rich domain and inclusive of the NLS [37,47]. This was further determined by transactivation analysis, in addition to direct RBP-Jk binding studies, in which RtaSTAD was further truncated to Rta aa 414 [37,47]. This Rta mutant, unlike RtaSTAD, was unable to rescue transactivation of the Mta promoter with RBP-Jk/VP16, nor was it able to form ternary complexes with RBP-Jk and promoter DNA in supershift assays [37,47]. Thus, these data suggested that the minimal Rta region required for binding RBP-Jk was aa 414-530; this domain functions in concert with Rta's DNA binding domain to stimulate RBP-Jk DNA binding to Rta responsive promoters. Nevertheless, the requirement for this domain is in contrast to an N-terminal region in Rta, between aa 170 and 400, which was shown to bind to RBP-Jk in solution, but was not sufficient alone for ternary complex formation and transactivation [37,47,48]. Taken together, the aa 414-530 region of Rta is required for both binding by RBP-Jk and stimulation of transactivation-competent ternary complex formation with promoter DNA.
Finally, in a study from our lab that bridged Rta CANT repeat recognition with its physical interaction with RBP-Jk at downstream promoters, it was revealed that RtaSTAD inhibited Rta-mediated transactivation and lytic replication, suggesting that RtaSTAD acted as a DN against WT Rta [35]. Thus Rta formation of mixed multimers was a required for its function. Further analysis showed that of all multimers, tetramers were sufficient to mediate the ability of Rta to transactivate genes [37,54]. Functional binding studies mapped the minimal tetramerization domain of Rta to aa 244 to 414 [37,54]. This region was notable for its inclusion of a 31 nt, N-terminal leucine heptapeptide repeat domain (LR) [37,54]. The KSHV Rta LR is similar to leucine zippers (LZs) in yeast, and shares a similar structure to Rta homologs in other primates, including three conserved leucines spaced at seven-residue intervals [37,54]. LZs are known to form alpha helix-based coiled coils and play a role in protein dimerization [37,54,108,109]. However, the KSHV Rta LR is also divergent from LZs in that it contains a high proline content; the LR overlaps with Rta's proline-rich region [37,54,108,109]. The five prolines within the LR are conserved among γ-herpesvirinae, and had originally been predicted to prevent coiled coil formation typically important for canonical LZ oligomerization [37,40,54,108,109]. As the KSHV Rta LR was necessary for tetramer formation, it was hypothesized that the region might enable this function without a need for the hypothetical coiled coil structure [37,54]. To ensure coiled coils weren't required for higher order Rta, the conserved leucines were mutated to prolines. The Rta-L3P mutant formed almost exclusively tetramers, confirming the nonessentiality for a typical LR structure in this activity [37,54]. Surprisingly, Rta-L3P was capable of WT levels of transactivation and reactivation [37,54]. It was concluded that Rta tetramers are essential for its transactivation potential and that, interestingly, the proline content within and beyond the LR, but not the LR's canonical secondary structures, may be important in determining Rta's higher order status-and perhaps broader, additional functions-based on their modification [37,54].
Given the body of evidence, a dynamic model for Rta transactivational function has been proposed: Rta protein forms tetramers and binds to RREs in viral and cellular promoters, alone or in conjunction with essential cofactors; straddling of Rta tetramers that contact multiple, flanking palindromic CANT DNA repeats, via binding of a novel Rta peptide motif to RBP-Jk, targets RBP-Jk to its element present in many Rta gene targets, allowing for the recruitment of additional coactivators and initiation of gene transcription [37,46,47,54,55]. Success of this transactivation program is critical to completion of the entire lytic cycle cascade, and relies on the interplay between Rta's interaction with cofactors and, likely, on guidance by putative, proline-directed modifications that regulate Rta to carefully define its activities throughout viral reactivation [2,4,7,37,42,46,49]. It is the recent report published by our lab describing one such putative proline-directed modification of Rta-regulation by proline isomerization-that is the major focus of this review.

Function, Regulation and Dysregulation of Pin1 Isomerase and its Novel Role in KSHV Lytic Reactivation
Posttranslational modifications are absolutely vital to the proper function of proteins within a cell, for signaling, conformation, interactions with other factors, stability, localization, DNA binding and transactivation, among many others. A number of potential modifications to Rta include phosphorylation, sumoylation, ubiquitylation and proline-directed modifications such as prolyl hydroxylation and prolyl isomerization [2,4,7,34,37,[42][43][44][45][46]54,55,64,68,110]. The demonstrated importance of prolines within Rta may not have been limited to a role in tetramerization, but could have broader consequences on Rta function.
Such modification is possible by peptidyl-prolyl cis/trans isomerases (PPIases) [111]. Isomerization of proline was first discovered as an important mechanism for proper protein function in the context of nascent protein folding [111][112][113][114][115]. The ability of primary amino acid structure to correctly fold into a functional conformation following ribosomal synthesis within the ER is largely dictated by the physical properties of the amino acids themselves. As trans form residues are solely synthesized by ribosomes, any protein that requires cis form residues would be unable to correctly fold and function. However, proline isomerization, by itself, is a rate-limiting process occurring at the multi-minute timescale [115][116][117]. A cell would be unable to survive if its protein contents took so long to mature.
Peptidyl-prolyl cis/trans isomerases (PPIases) are highly conserved cellular catalysts that bind to and isomerize prolines at millisecond timescales, thus, effectively allowing for rapid, physiologically-relevant protein folding and function [111,113,115,116]. They are found in all organisms, including bacteria. There are four classes of PPIases: cyclophilins (Cyps), FK506binding proteins (FKBPs), parvulins and the protein Ser/Thr phosphatase 2A (PP2A) activator (PTPA) [111,113,115,116]. The initial characterization of Cyps and FKBPs revealed them as targets for immunosuppressive and anticancer drugs cyclosporine A, FK506 and rapamycin, though it was soon reported that this was unrelated to their PPIase activity [111,113,114,116]. Further, their biological significances were questioned due to their redundancy, the presence of dedicated chaperone molecules and that disruption of single or multiple PPIase genes did not affect cell viability [111,114,118]. It did not appear that PPIases were essential general factors, although in subsequent years, important specific interactions were described. For FKBPs, for instance, FKBP 12 was found to associate with ryanodine and inositol 1,4,5-triphosphate (IP3) receptor subunits and inhibit TGF-β receptors [111,[113][114][115]119]. Interestingly, cyclophilins appear to be important in the pathogenesis of various virus, including HIV-1, hepatitis C virus (HCV) and human cytomegalovirus (HCMV, human herpesvirus 5) [111,[113][114][115]120].

Human PPIase and Cell Cycle Regulator Pin1
In 1996, a new class of PPIases, parvulins, was identified as the result of a screen in Aspergillus nidulans for direct binding inhibitors of the essential mitotic kinase never in mitosis A (NIMA) [116,121,122]. The screen isolated three human proteins, one of which was peptidyl-prolyl isomerase NIMA interacting protein (Pin)1, a small 18 kDa protein determined to be a novel PPIase containing characteristic N-terminal WW substrate binding and C-terminal PPIase catalytic domains ( Figure 2) [121,[123][124][125]. Despite sharing the same basic domains with similar enzymatic activity, Pin1 was found to have a dramatically different structure from the other PPIase classes (which themselves are structurally distinct) [111,115,123,126]. Pin1 showed conservation from yeast (where it is known as Ess1) to humans [111,115,116,121,123,128]. Its role as a suppressor of NIMA-induced mitotic catastrophe marked the first non-"housekeeping" function ascribed to isomerases, in that it was both essential for cell viability in general, and as a regulator of mitosis specifically [121]. Additional characterization of Pin1 showed an intriguing specificity for peptidyl-prolyl motifs that required phosphorylation of the N-terminal peptidyl residue for recognition and for isomerization [115,116,123,129]. Peptidyl residues must be, in the case of Pin1, phosphoserines or phosphothreonines. It appeared that Pin1 WW domains bound to pS-P or pT-P (known as "pS/T-P motifs") in targets by recognition of the phosphorylation, which reduced the double-bonded character of the oxygen N-terminal to the peptide bond ( Figure 3A) [115,116,123,126,130]. As for all PPIases, this significantly reduced the torsion barrier that restricted rotation about the peptide bond from trans to cis forms (or vice versa), rapidly speeding up the conversation rate by several orders of magnitude [111,115,116,126]. (A) Cis-trans isomerization is an intrinsically slow process; PPIase catalysis at pS/T-P motifs reduces this timescale from minutes to milliseconds, by binding to the phosphoresidue N-terminal to proline, which the double-bonded oxygen and thus, the torsion barrier preventing conversion, allowing for a 180° rotation about the bond. (B) Since only trans-form of prolines bcan e acted on by regulators, isomerization can "lock" phosphorylation, and therefore a substrate's function (such as, for example, stability of the human T cell leukemia virus (HTLV)-1 Tax oncoprotein, above), in place. As such, Pin1 is regarded as a timer of a variety of critical cell cycle and signaling even ts, including for those involving both cellular and viral regulators.  [115], and from [131].
The findings pertaining to Pin1's phosphorylation dependency were significant for a few reasons. First, phosphorylation greatly slows the spontaneous cis-to-trans conversion rate and makes catalyzed, reversible isomerization essential for proteins requiring a particular conformation for function [115]. Second, no other PPIase recognized motifs that contained phosphorylated residues [111,115,123]. Third, and most importantly, action at phosphorylated moieties strongly implied that Pin1 has broadly-applicable regulatory potential at a previously unrecognized, postphosphorylational level [115,130,132,133]. This is because kinases and phosphatases are involved in numerous cell signaling events and are capable of targeting trans form serines and threonines only [115]. Thus, Pin1 binding to specific motifs within important regulatory or effector proteins render phosphorylation (or lack thereof) "locked in" by a switch to cis form, resistant to kinase or phosphatase activity. Protracted "on" or "off" states under the control of Pin1 isomerization, then, alter protein function and subsequently the conduct of their constituent pathways ( Figure 3B).

Dysfunction of Pin1 Is Often Associated with Tumorigenesis
It was quickly borne out that Pin1 was indeed an integral cell-signaling regulator [115,121,123,130]. Perhaps its best-studied, and most important single interaction lies with Cyclin D and the G1/S checkpoint, a prime example of Pin1's postphosphorylational control [115,119,130,[133][134][135][136]. Pin1's role, however, extends beyond cell cycle signaling; it is a truly pleiotropic enzyme with a wide array of substrates (Table 1).
Finally, Pin1 was also more recently implicated in virally-derived tumors as well as in viral pathogenesis in general. For instance, HIV-1 capsid (CA) protein uncoating, a process essential for subsequent reverse transcription and viral replication, is mediated by Pin1 activity [154,155]. So too does Pin1 interact with and inhibit APOBEC3G, a cytidine deaminase and antiviral factor that incorporates into HIV-1 virions to block viral replication, as well as stabilizes the HIV-1 integrase for incorporation of virus into host genomes [156]. Pin1 promotes ubiquitylation of IRF3, inhibiting the host IFN innate antiviral response and promoting susceptibility to viral infection [157]. For hepatitis C virus, Pin1 interacts with viral nonstructural proteins NS5A and NS5B to enhance HCV replication [158]. Hepatitis B virus (HBV) encoded protein X (HBx) stability is mediated by Pin1, which is associated with HCC [159]. Notably, Pin1 stabilizes the human T cell leukemia virus 1 (HTLV-1) oncoprotein Tax, a transcription factor similar to KSHV Rta, at least thematically, in that it transactivates downstream viral promoters for productive lytic replication and pathogenesis [131,133,160,161]. Stabilization allows Tax to interact with IKKγ and contribute to NF-κB-mediated cell transformation [161].

Pin1 Has a Novel Role in KSHV Lytic Reactivation
Pin1 has been found to play a role in herpesviral pathogenesis as well. In HCMV infection, Pin1 is recruited to aid reorganize nuclear lamin A/C upon phosphorylation of the lamina by viral kinase pUL97 and cellular PKC [162]. Pin1 also associates with a number of proteins that could play roles in viral egress, including microtubule binding protein tau, actin filaments (which are known to be incorporated into virions during assembly) and, interestingly, kinesin-related protein KRMP1, a motor protein similar to kinesin or myosin that may play an important role, together with Pin1, in regulation of mitosis, potentially through the transport of Pin1 and other mitotic substrates [119,133,163,164]. And in a recent study by Narita et al., Pin1 was found to bind to γ-herpesvirus EBV protein BALF5, the catalytic subunit of the viral DNA polymerase, and enhance EBV replication [165].
Recently, our lab investigated a putative interaction between KSHV Rta and PPIase Pin1 in the regulation of Rta-mediated lytic reactivation at multiple stages of the lytic cycle cascade. In silico alignment analysis of the transcription activation factor (TAF) 50 superfamily revealed that Rta homologs share a consensus of 134 amino acids (residues conserved between KSHV Rta and at least one additional member). Proline consists of 38 of these amino acids, accounting for 16.7% of all of Rta's conserved residues ( Figure 4) [40,166]. The high degree of conservation supported their putative functional significance. We reasoned that modification of prolines may regulate Rta's efficiency in transactivating target genes, licensing viral DNA replication and interacting with protein partners-which could drive the latency-lytic cycle balance in favor of productive replication.
In our report, we demonstrated an interaction between cellular isomerase Pin1 and KSHV lytic switch Rta. We showed that Pin1 is expressed and active in infected PEL cells after lytic cycle induction, and that Pin1 directly interacts with Rta in vitro and in infected cells, most likely at one of Rta's putative conserved Pin1-recognition (pS/T-P) motifs [166]. Pin1 did not, however, interact with the essential Rta cofactor and Notch effector RBP-Jk by GST pulldown assay [166]. Pin1 enhanced Rta transactivation at two viral promoters in transient transfections [166]. Cotransfection of Rta with Pin1 appeared to result in enhanced redistribution of Rta from punctae to strong pan-nuclear expression in the majority of cells that coexpressed Pin1 (89%) [166]. This effect seemed to involve minute amounts of Pin1, as most coexpressed cells with Pin1 even modestly over background displayed Rta relocalization [166]. Overall Rta expression was also markedly stronger between punctae and pan-nuclear localization. In WT and Rta-inducible, virally-infected PEL, iSLK and Vero cells, we showed that Pin1 has a time-dependent effect on lytic reactivation, enhancing early-stage but inhibiting late-stage lytic cycle function [166]. Early-stage enhancement was shown via Rta-mediated DE transactivation and viral DNA-based experiments that overexpress or ablate Pin1. Late-stage inhibition by Pin1, meanwhile, was shown via reactivation experiments in WT and Rta-inducible PELs, in a Rta-inducible iSLK BAC16-based cell line system in which the viral allele of Rta is rendered defective by insertion of a stop codon (BAC16-RTAstop), and finally in a new, two-step KSHV reporter Vero-based cell line system developed by Gantt and colleagues that contain the secreted alkaline phosphatase (SeAP) gene under the control of an upstream tetracycline responsive element (TRE) promoter [167]. Together, those studies suggest that expression of late gene K8.1, and subsequent infectious virion release, are markedly inhibited [166,168]. Our published report showed that KSHV co-opts Pin1 function as a molecular timer, where by Pin1 enhances Rta expression, Rta transactivation activity at Rta-responsive promoters and Rta-mediated viral DNA replication, but inhibits late gene synthesis and virion production [166]. We propose that this dichotomy of Pin1 function can impart KSHV with a prosurvival, abortive lytic reactivation pathway, one which we hypothesize may regulate viral pathogenesis through the expression and activity of lytic cycle oncoproteins. To our knowledge, we mark the first discovery of an interaction between a DNA virus transcription factor and Pin1.

Ectopic Pin1 Is Sufficient to Induce Rta Expression: Putative Mechanisms
Early Pin1 activity may be important due to its sufficiency to induce Rta expression. Pin1 is known to affect protein expression in a number of ways. First, it could upregulate Rta transcription through welldescribed signaling pathways. Chief among them are c-Jun and c-Fos, which constitute the transcriptional regulator activating protein (AP)-1, as well as hypoxia inducible factor 1α (HIF-1α, an important regulator within the hypoxia response [89,92,169,170]. In PEL cells, HIF-1α is always active due to LANA inhibition of VHL [171]. HIF-1α can then activate Rta expression as well as Rta co-activators [69,89,90,172]. It is intriguing that Pin1 engages in a positive feedback mechanism with HIF-1α during the hypoxia response [146,147,173]. Additionally notable is that the ER stress response sensor BiP is upregulated by Pin1, an activity that is conserved with other PPIases in their role as protein folding regulators [174]. Meanwhile, AP-1 involvement in potential Pin1-dependent Rta transactivation has additional implications. Pin1 function upstream of Rta IE gene expression, and through the AP-1 pathway, is highly similar to TPA's mechanism of lytic induction [81,134,151,152,175]. In Pin1 −/− MEFs, TPA induction of the AP-1 pathway was found to be much weaker than in Pin1 +/+ MEFs [175]. Second, Pin1 could affect transcriptional elongation and posttranscriptional splicing [115,128,137,176]. This could occur via Pin1's described interaction with RNA polymerase (RNAP) II [137,176,177]. Pin1 has been shown to play a role in control of carboxyl-terminal domain (CTD) phosphorylation [177]. The combination of phosphorylation and cis/trans isomerization designates the so-called "CTD code" that coordinates proteins involved in RNAP II-mediated events, including mRNA processing [128,177]. Future studies of Rta transcriptional and posttranscriptional control in the presence of Pin1, as well as Pin1 promoter binding and activity studies in infected PELs, will help confirm and define the above-suggested interplays.

Pin1 Directly Binds to Rta and Enhances Rta Transactivation
In our report, we found that Pin1 and Rta directly interact in vitro and in infected cell lysates. As with most of its protein substrates, Pin1 could enhance expression independently of its transcriptional effects through direct stabilization of Rta protein. Pin1 often stabilizes proteins that may otherwise be ubiquitylated and targeted for proteosomal degradation, such as Cyclin D [115,133,178]. The most applicable examples of this are the viral Tax and HBx oncoproteins and transcription factors of HTLV-1 and hepatitis B virus (HBV), respectively, as previously discussed [159,161]. These factors are prevented from degradation by conformational changes induced due to prolyl isomerization, allowing their protein levels to accumulate for efficient downstream activities, including transactivation and productive viral replication. It is possible that Pin1 could affect Rta stability by either inhibiting Rta auto-ubiquitylation via Rta's E3 ubiquitin ligase domain, or by enhancing Rta-induced degradation, also through its E3 ligase activity, of Rta repressors such as K-RBP and IRF7 [63,64,68]. Indeed, these repressors bind to Rta within proline-rich regions containing putative Pin1 isomerization motifs; Pin1 activity could prevent their association.
As we observed that the Pin1 interaction appeared to be stronger with full-length WT Rta, we cannot rule out that Pin1 has multiple binding motifs within Rta. First, Pin1 is known to bind to a number of its targets at more than one motif; this includes p53, c-Jun, Nanog and Akt [126,133,134,[178][179][180]. Second, Pin1 binding and isomerization activities are separable and complex. Binding motifs can be recognized by each domain and acted upon with different specificities [111,115,116,123,130,181]. In other words, Pin1 can bind one motif, but very well isomerize another.
Other possible motifs could also have binding and/or isomerization specificity, including Thr449, Thr515, Ser634 (which is a fully conserved Pin1 motif) and Ser636 ( Figure 5) [166]. The latter three, in this case, are interesting putative motifs, as they are the only known potential Pin1 sites to date that has been previously shown to be phosphorylated in in vitro binding assays or in infected cell-based Western blots, by Rta transactivation inhibitor hKFC (for Thr515) and CDK9 (Ser634/636), respectively (and it bears mentioning that the other, putative CDK binding sites, at Thr449, Thr540, Thr628, Ser644 and Ser650, are also all putative Pin1 sites) [42,44,45]. CDK9 kinase activity on Rta is notable because CDK9 is a catalytic subunit of positive transcription elongation factor b (P-TEFb), which associates with the promoter-paused RNAP II's CTD and activates transcriptional elongation [44]. Rta likely recruits CDK9 to viral promoters, where the kinase licenses transcription as well as positively regulates Rta activity. Pin1, which as previously discussed, binds to the CTD and regulates transcriptional elongation, is known to bind to CDK9-phosphorylated substrate hSpt5, an elongation inhibitor [137,182]. Thus, Pin1 interactions with both CDK9 targets, hSpt5 and Rta, could enhance RNAP II transcriptional elongation at KSHV gene promoters. While it is tempting to suggest that these could represent bona fide pS/T-P motifs, the cytosolic localization of hKFC makes an Rta-regulating Pin1 modification through hKFC in infected cells suspect, while for CDK9, in light of the lack of Pin1 binding to the Rta aa 525-691 truncation mutant, a true Pin1 motif beyond aa 525 appears less likely [45,166]. Nevertheless, Pin1 binding at Ser634/636 in infected cells, alone or combined, could still be possible, and future functional binding analysis with Pin1 motif mutants of Rta will be required to clarify this interaction. Taking into account the intricate regulation by Pin1 reported throughout this review, multiple binding sites could provide Pin1 with a combinatorial influence on Rta function, much as Pin1 coordinates the CTD code of RNAP II, based directly on the phosphorylation status of certain motifs. This control could be one explanation for the divergent effects of Pin1 during the lytic cycle, which will be further addressed below.
Pin1 binding to Rta could mediate a variety of different effects on Rta beyond stability, including tetramerization. Bearing in mind the importance of conserved prolines on Rta higher-order structure, that Pin1 binding to the Rta aa 170-400 region overlaps with the tetramerization and proline-rich domains (Figure 1), the putative Pin1 motif at Thr388 ( Figure 5) and that previous data showed that RBP-Jk also binds to Rta aa 170-400 in solution [47,48], it is reasonable to suggest that Pin1 binding to Rta could regulate Rta's tetramer formation, allowing it to interact with RBP-Jk and transactivate downstream viral and cellular genes [166]. . Rta protein has putative, conserved Pin1 motifs. Blue bars indicate 15 putative S/T-P motifs, sites for Pin1 binding and isomerization, which are characterized by a phosphoserine or phosphothreonine immediately preceding a proline. Letters indicate aa that are parts of possible Pin1 motifs that are phylogenetically conserved between KSHV Rta and at least one additional γ-herpesvirinae family member ORF50 homolog (part of the TAF50 protein superfamily). Motif bars colored in red indicate those that are known to be phosphorylated (T515, S634 and S636; see Figure 4). Putative Pin1 motifs T449, T540, T628, S644 and S650 are also putative phosphorylated residues. Boxes at bottom show alignments of proline-rich and RBP-JK binding regions of Rta. Numbers indicate aa position. +++ = positively-charged aa-rich, LR = leucine heptapeptide repeat domain, S/T = serine/threonine-rich, hyd DE hyd = hydrophobic/charged/hydrophobic aa-rich, NLS = nuclear localization signal sequence, P = conserved proline, S = conserved serine, T = conserved threonine, M = fully conserved S/T-P motif. Figure modified from [166].
In the literature, Pin1 has been previously reported to affect protein multimerization, in that case destabilizing IRF3 homodimer formation [157]. Pin1 also has a known role in upregulating Notch1 activity through enhanced cleavage of NICD by γ-secretase, which it could perhaps do in KSHV-infected cells in concert with the reported stabilization of NICD by LANA or activation of Notch4 by vGPCR [101,147,183]. NICD can weakly bind to RBP-Jk alone for modest transactivation at viral promoters, but can act synergistically with RtaΔSTAD due to the effect of NICD's transactivation domain [47].
It is important to point out that, although we tested and confirmed that Pin1 greatly enhanced Rta-mediated transactivation and DE gene expression using well-studied promoter markers nut-1 and Mta, we did not directly observe Pin1's effect on KSHV's lytic cycle oncoproteins, such as vGPCR or vIL-6, etc. Therefore, while our data suggest that Pin1 affects DE gene expression nonspecifically, we cannot make concrete conclusions. The same is true in regards to Pin1's effect on KSHV oncogenic properties, including VEGF production and angiogenesis, cell cycle disruption and apoptotic subversion. Could Pin1 expression and/or activity distinguish Rta-initiated reactivation cascades that differ in expression of replication protein from oncoproteins? If so, could Pin1 activity determine the oncogenicity of KSHV infection? As the purpose of our investigation was to identify and characterize the qualitative role of Pin1 on the Rta lytic switch and on lytic reactivation, further studies addressing such questions will develop Pin1's lytic cycle-based molecular mechanisms as well as the broader phenotypic and tumorigenic ramifications of these, and other, activities at multiple stages of the KSHV life cycle, including in de novo infection and egress.
The mechanism of how, exactly, Pin1 strengthens Rta-mediated transactivation and DE gene expression is unclear and the subject of ongoing examination. Additional, indirect enhancement of Rta transactivation efficiency could be aided by Pin1's function with RNAP II in transcriptional elongation or termination, or in posttranscriptional processing and splicing [128,137]. These functions, especially the latter, could cooperate with Mta. Like Pin1, Mta is enriched at nuclear speckles and is involved in posttranscriptional elongation and cellular factor-dependent viral pre-mRNA splicing [95,96,184]. Mta also stabilizes nascent viral transcripts and facilitates export of intronless viral transcripts, which account for ~70% of all KSHV mRNAs [95,96,184]. Pin1 and Mta could together coordinate RNAP II transcriptional progression in conjunction with processing factors stored in nuclear speckles, with Mta and Pin1 first directly enhancing Rta function at promoters, followed by Mta stabilizing elongating transcripts, Pin1 enabling proper termination via CTD code modification and then Mta shuttling intronless mRNAs out of the nucleus for efficient translation. Taken together, along with the context-dependent Pin1 motifs scenario, Pin1 could be part of a multi-tiered regulatory loop consisting of different functional consequences for Rta expression and for Rta transactivation.

Pin1 Enhances KSHV Lytic DNA Replication
Results from our report also suggested that inhibition of Pin1 drastically reduces the rate of replication as it proceeds through the lytic cycle. This could simply be due to functional carryover from reduced DE gene synthesis. However, the strong impact of juglone on replication suggests that loss of Pin1 may have a broader effect than transactivation alone. We can further suggest, then, that Pin1's transactivation enhancement of Rta could extend to Rta's association at oriLyts and its role in lytic replication in concert with K-bZIP [7,37,61]. Pin1 binding to Rta could enhance Rta's ability to recognize its elements at oriLyts, to recruit basal DNA replication factors in a manner analogous to Rta's recruitment of transcription factors at viral promoters, or to interact with K-bZIP directly. Pin1's effects on Rta-mediated transactivation and replication are probably based on a single modification that simultaneously enhances both processes, although additional work will need to be done to rule out a more complex regulatory mechanism.

Pin1 Represses KSHV Late Gene Expression and Virion Production
We expected Pin1 to continue to act as an Rta enhancer and upregulate productive lytic reactivation as assessed by release of infectious virus, which often positively correlates to viral replication. Instead, data from our report strongly suggested that Pin1 inhibits virion production [166]. Rather than acting as a positive cofactor that enhances Rta-mediated transactivation and replication, Pin1 may actually be a complex, bimodal regulator of lytic reactivation, as it later acts as a negative cofactor that represses virion production and release.
A number of potential hypotheses could be proposed that address the manner in which Pin1 inhibits late gene synthesis following Pin1's enhancement of viral DNA replication. Pin1, despite its oncogenic functions, also has interactions with a variety of cellular or viral regulators that could negatively impact lytic cycle progression. One intriguing explanation for Pin1's repression of late gene synthesis comes from its potential cooperation, as discussed above, with Mta during posttranscriptional and splicing regulation. Mta binds to intronless viral mRNA for efficient export into the cytosol for translation [95,96]. The cellular polyadenylation-binding protein C1 (PABPC1), with a natural localization in the cytosol, protects polyA transcripts from cytidine deadenylases with nanomolar affinity and enhances both mRNA nuclear export and translational initiation [60,96]. Upon expression of the lytic cycle, viral shutoff exonuclease (vSOX) relocalizes PABPC1 to the nucleus, where it binds and stabilizes cellular and viral polyA mRNA as before, but sequesters these transcripts in the nucleus [60,96]. As Mta binding to intronless viral mRNA cannot overcome PABPC1 sequestration, this would, in effect, shut down all polyA transcript export and translation (which, recall, accounts for 70% of all viral mRNA) [60,96]. However, nut-1/PAN, the DE noncoding transcript (at 500,000 copies per virally-reactivated PEL cell, by far the most abundant, at upward of 80% of all cellular polyA mRNA), has been found to bind to PABPC1 and titrate the protein away from polyA mRNA; since Mta binds only intronless viral mRNAs, it was hypothesized that the cooperation of both nut-1 and Mta allows for preferential nuclear export of viral transcripts, while PABPC1 still drastically slows the export of cellular transcripts [59,60,96]. Thus, the vast majority of protein produced during lytic reactivation is viral. Notably, however, the loss of nut-1 accumulation has been observed to cause deficient late gene synthesis, with concomitant loss of virion production, even despite normal Rta expression, transactivation and viral DNA replication [60].
As Pin1 is involved in many of the same processes as Mta, one could postulate that Pin1 may interfere in some way with PABPC1 protein interaction with nut-1 in the nucleus following vSOX activation; this would achieve the same functional result as nut-1 deficiency, since inhibition of nut-1 expression does not appear to occur based on our transactivation and DE gene expression-based data. This modulation of nut-1 would prevent late gene synthesis without impacting any previous lytic cycle stage nor the suppression of cellular gene translation. The scenario also allows for a much simpler mechanism of bimodal Pin1 function without the requirement for any direct, mid-lytic cycle regulatory alteration of Pin1 activity, although combinatorial Pin1 binding to Rta could very well still occur. Nevertheless, Pin1's dynamic regulation could be "built in" to the lytic cycle program, one in which enactment of a single modification for each of a limited number of viral factors, particularly Rta, could achieve a complex, tightly-coordinated progression of events. Accounting for such stage-specificity, Pin1 blockade of late gene synthesis following Rta induction could be due to potential time-dependent cofactors, such as particular DE protein interaction with Pin1; unknown differences at late gene promoters that prohibit Rta transactivation in concert with Pin1; upregulation of cellular or viral factors following the onset or completion of viral DNA replication; or aforementioned combinatorial Pin1 activity that directly affects Rta function.

Molecular Timing Model for Pin1's Effects on KSHV Replication and Pathogenesis
Taking the culmination of results from our report together, each of the above possibilities could allow for Pin1 to function in a divergent manner as a postreplication lytic cycle inhibitor capable of reducing the efficiency of, or altogether counteracting, prolonged virion release and cell lysis. Put another way, Pin1 is co-opted by KSHV to regulate the timeframe of reactivation and the balance between abortive and productive lytic reactivation. Pin1 overexpression may shift this balancing act in favor of repression, while too weak Pin1 signaling might not activate Rta expression to begin with. Thus, a "Goldilocks" level of Pin1 regulatory activity during the lytic cycle may be required for the cycle's initiation, progression and completion, a role that possibly evolved as a prosurvival, immunoevasive measure that emphasizes DE gene expression and clandestine viral replication within an infected cell population.
This was in keeping with a dosage-dependent timing mechanism that may allow for only a subset of cells induced for Rta expression to advance through the full lytic cycle. Indeed, reactivation occurs in a small subpopulation of KSHV-infected tumor cells, produces oncogenic DE gene products that are believed to be essential for tumor growth and is governed by inefficient Rta activity as the vast majority of Rta-expressing cells do not coexpress late gene markers, such as K8.1 [4,7,50]. Molecular hijacking of a conserved cellular timer that has subtle, but powerful effects on a variety of oncogenic and pro-viral processes could have evolved as a protective rheostat that minimizes noise for lytic switch induction. Fine-tuning the ratios between latency, and abortive and productive replication, could mitigate spurious and potentially self-limiting "runaway" virion production, host cell lysis and immune system activation that disrupt the local infected cell microenvironment, prevent longterm cellular stability of viral episomes and risk overall virus survival in the host.
We proposed a dynamic molecular timing model in which Pin1 upregulates Rta expression, transactivation and viral replication ab initio, but then later suppresses optimal, productive lytic replication ( Figure 6). If proven correct, by opening up a threshold-or kinetics-dependent "window" that licenses the initiation and progression of reactivation, KSHV has evolved an exquisitely balanced, prosurvival lytic program: co-option of a multifunctional cellular timer, Pin1 isomerase, maximizes Rta-mediated transactivation of viral lytic cycle genes, and then conversely protects against unchecked infectious virion production that would otherwise compromise host cell integrity and viral immune evasion for the vast majority of KSHV-infected cells that may reside within tumors. Our findings therefore point to Pin1 as an attractive antiherpesviral drug candidate that could be of potentially efficacious use in the treatment of HIV-1-positive and immunocompromised patients at risk for, or afflicted with, KSHV-derived malignancies. Figure 6. Pin1 acts a novel KSHV lytic cycle timer through regulation of Rta expression and downstream activity. Disease models of cells infected with latent KSHV show that a small subpopulation undergo reactivation, which is thought to promote tumor growth. The lytic cycle cascade begins with Rta and other immediate-early (IE) protein expression, followed by Rta-mediated transactivation of delayed-early (DE) genes, such as Mta, and which include DE oncoproteins and viral DNA replication factors. Upon completion of Rta-dependent viral replication, late gene synthesis proceeds with structural and glycoprotein expression, such as K8.1. Finally, assembly and egress of infectious virions allows for dissemination within the host and to other individuals. It is likely that Pin1 isomerase modulates Rta activity during reactivation. During early reactivation events (Rta DE transactivation, viral replication), Pin1 strongly enhances Rta function. However, by an unknown mechanism within the lytic cascade, Pin1 transitions into an inhibitor of late events (late gene synthesis, infectious virus release), halting productive reactivation. Thus, Pin1 functions as a molecular timer. Pin1 is known to control strength and duration of an array of normal and pathological cellular signals, and we believe Pin1's timing activity is co-opted by KSHV to allow for an evolutionarily-advantageous, nonproductive window allowing for DE gene expression while protecting against cell lysis and immune response activation. Figure modified from [166].