Clinical Effects of Interproximal Contact Loss between Teeth and Implant-Supported Prostheses: Systematic Review and Meta-Analysis

: Dental rehabilitation with implants is a clinical reality in clinical practice. The Interproximal Contact Loss (ICL) between implant-supported prostheses adjacent to natural teeth is a relatively common occurrence. This systematic review and meta-analysis aims to evaluate the possible clinical effects of the periodontium regarding the ICL between teeth and implanted-supported prostheses. We also identified the main ICL assessment tools described in the literature. This study was registered on the PROSPERO (CRD42023446235), was based on the PICO strategy, and followed the PRISMA guidelines. An electronic search was carried out in the PubMed, B-on, Google Scholar, and Web of Science databases without setting a time limit for publications. Only systematic reviews and comparative clinical trials were included and analyzed. Nineteen publications were eligible for meta-analysis, with thirteen retrospective and six prospective clinical trials. A total of 2047 patients and 7319 prostheses in function were evaluated, and ICL was found in 51% with a confidence interval of 0.40 to 0.61. As ICL assessment tools, dental floss was used in 65%, matrices were used in 30%, and X-ray images were used in 5% of cases. The clinical follow-up ranged from 1 to 21 years, with 50% between 1 and 3 years, 25% between 3 and 10 years, and 25% between 10 and 21 years. ICL was found to occur more frequently in the mandible. No statistically significant difference existed between the anterior (55%) and posterior (47%) oral regions. On the mesial surface, ICL ranged from 13% to 81.4%, possibly due to the different follow-up periods and the diversity of methods used in the assessment. No differences were found for ICL between single or multiple implanted-supported prostheses. Food impaction was the most common effect of ICL and was more prevalent on the implant-supported prosthesis’s mesial surface in the mandible’s posterior region. There was evidence of peri-implant mucositis but without progression to peri-implantitis, and the form of retention or the number of elements was not relevant


Introduction
Natural teeth show physiological tooth migration and movements that occur from tooth eruption, including functional inclination in the alveolus and the possibility of adapting to physiological and/or functional needs, whether horizontally, vertically, or rotationally.Occlusion is an important factor in maintaining interproximal contact surfaces and the physiological homeostasis of the periodontium [1,2].
The periodontal ligament keeps the tooth in union with the bone, allowing physiological movement.In contrast to teeth, implants have direct contact with the bone and are not subject to the mobility experienced by teeth.In implant rehabilitation, whether single or multiple, when adjacent to a natural tooth, this difference in behavior between the mobility of the tooth and the immobility or ankylosis of the implant is evident [3][4][5].Figure 1a shows the relationship between an implant located in position 35 and perfect harmony in interproximal contact between the implant prosthesis and the adjacent natural teeth, with a healthy peri-implant and periodontal region.
Prosthesis 2024, 6, FOR PEER REVIEW 2 The periodontal ligament keeps the tooth in union with the bone, allowing physiological movement.In contrast to teeth, implants have direct contact with the bone and are not subject to the mobility experienced by teeth.In implant rehabilitation, whether single or multiple, when adjacent to a natural tooth, this difference in behavior between the mobility of the tooth and the immobility or ankylosis of the implant is evident [3][4][5].Figure 1a shows the relationship between an implant located in position 35 and perfect harmony in interproximal contact between the implant prosthesis and the adjacent natural teeth, with a healthy peri-implant and periodontal region.The space opening induced by the implant-supported prosthesis adjacent to the natural tooth, also known as Interproximal Contact Loss (ICL), represents a typical clinical situation and has been reported in the literature, especially in systematic reviews, retrospective clinical studies, prospective publications, clinical case reports, and some classifications have been proposed [6,7].Some ICL complications, such as Food Impaction (FI), caries lesions in the adjacent tooth, gingival inflammation, periodontal changes, and, in some studies, loss of bone support, have been reported.Various methods have been proposed to assess the measurement of the interproximal contact or ICL [8,9].
In order to prevent the installation and/or progression of those alterations, it is necessary to perform the monitoring of the interproximal space over time and adjust the alterations as soon as possible [10,11].It is important to highlight the need for the evaluation of diagnostic and treatment methods in order to avoid ICL triggering and prevent its occurrence.This is shown in Figure 1b: Due to the lack of a contact point on the mesial face of the implant prosthesis adjacent to the natural tooth, Food Impaction (FI) can occur, causing damage to the gingival tissue and promoting demineralization of the tooth with the deposition of decayed tissue and the appearance of changes in the gingival mucosa that can evolve and generate bone loss in the damaged area [11][12][13][14][15].
For those purposes, this study aims to evaluate the possible clinical effects of the periodontium on the ICL between teeth and implanted-supported prostheses and also to identify the main ICL assessment tools described in the literature.The space opening induced by the implant-supported prosthesis adjacent to the natural tooth, also known as Interproximal Contact Loss (ICL), represents a typical clinical situation and has been reported in the literature, especially in systematic reviews, retrospective clinical studies, prospective publications, clinical case reports, and some classifications have been proposed [6,7].Some ICL complications, such as Food Impaction (FI), caries lesions in the adjacent tooth, gingival inflammation, periodontal changes, and, in some studies, loss of bone support, have been reported.Various methods have been proposed to assess the measurement of the interproximal contact or ICL [8,9].
In order to prevent the installation and/or progression of those alterations, it is necessary to perform the monitoring of the interproximal space over time and adjust the alterations as soon as possible [10,11].It is important to highlight the need for the evaluation of diagnostic and treatment methods in order to avoid ICL triggering and prevent its occurrence.This is shown in Figure 1b: Due to the lack of a contact point on the mesial face of the implant prosthesis adjacent to the natural tooth, Food Impaction (FI) can occur, causing damage to the gingival tissue and promoting demineralization of the tooth with the deposition of decayed tissue and the appearance of changes in the gingival mucosa that can evolve and generate bone loss in the damaged area [11][12][13][14][15].
For those purposes, this study aims to evaluate the possible clinical effects of the periodontium on the ICL between teeth and implanted-supported prostheses and also to identify the main ICL assessment tools described in the literature.

Materials and Methods
This systematic study formulated the research question, "Does the interproximal contact loss influence the outcomes for periodontium and other clinical effects?", based on the PICO model: Population-patients who received single or multiple implanted-supported prothesis adjacent to natural teeth, Intervention-assessment tools to evaluate and compare interproximal spaces between natural teeth and prostheses on implants, Comparison or Control-interproximal spaces between natural teeth, and Outcome-clinical effects for the peri-implant and periodontal tissues of the probable loss interproximal contact between the natural tooth when adjacent to implanted-supported prosthesis over time.The following null hypothesis was formulated: the loss of proximal contact between the natural tooth and the prosthesis on implants has no clinical effects on the periodontium.This systematic review was registered on the International Prospective Register of Systematic Reviews (PROSPERO, CRD42023446235) and followed the PRISMA guidelines as described in Figure 2 [16].
This systematic study formulated the research question, "Does the interproximal contact loss influence the outcomes for periodontium and other clinical effects?", based on the PICO model: Population-patients who received single or multiple implanted-supported prothesis adjacent to natural teeth, Intervention-assessment tools to evaluate and compare interproximal spaces between natural teeth and prostheses on implants, Comparison or Control-interproximal spaces between natural teeth, and Outcome-clinical effects for the peri-implant and periodontal tissues of the probable loss interproximal contact between the natural tooth when adjacent to implanted-supported prosthesis over time.The following null hypothesis was formulated: the loss of proximal contact between the natural tooth and the prosthesis on implants has no clinical effects on the periodontium.This systematic review was registered on the International Prospective Register of Systematic Reviews (PROSPERO, CRD42023446235) and followed the PRISMA guidelines as described in Figure 2 [16].

Inclusion Criteria, Exclusion Criteria and Eligibility
Prospective and retrospective observational clinical studies in humans with a followup of at least 1 year, with the presence of implant-supported dental crowns adjacent to natural teeth, were considered, which included assessment tools for the determination of interproximal space/contact, measurement of the contact gap, location of the gap in the oral cavity (maxilla, mandible, anterior, and/or posterior oral region), type of rehabilitation retention and number of prostheses (single or multiple), and clinical effects of the ICL.We excluded experimental laboratory studies, animal studies, case reports, and studies that did not include clinical data with follow-up for at least one year.

Search Strategies
Searches were carried out using the electronic databases PubMed/MEDLINE, B-on, Google Scholar, and Web of Science.The search strategy was conducted by two examiners (JCN and GB), and data collection was based on keywords and the Boolean operators "AND" and "OR": (dental implant or dental implants or implant-supported prosthesis) and "(loss of proximal contact or loss of interproximal contact or open contact or adjacent to natural tooth)" and "(food impaction)".

Selection and Evaluation of Studies
The Rayyan online platform was used to manage the references [17].After selection, the articles were screened, and duplicates were excluded by the two examiners.The titles were then read, and the exclusions were made.The abstracts were read by the two examiners (JCN and GB), and when there were discrepancies, a third examiner (BL) was consulted.Potentially eligible texts were read by both researchers (JCN and GB), and at all stages, the Rayyan software was blinded (https://rayyan-ios.soft112.comaccessed on 16 May 2024).The JCN researcher was responsible for deciding whether to include or exclude articles, and the second researcher (GB) was responsible for reviewing the information.Using the snowball technique, new articles were included after reading the bibliographical references of the articles selected for analysis.After this stage, the selected articles were exported to the Mendeley desktop 2.93.0 version.
Two thousand and twenty-one publications were collected, which, after excluding duplicates, resulted in 376 eligible papers.After reading the title, 78 studies were selected for evaluation of the abstract, and then a total of 30 publications were excluded.A total of 48 articles were identified for full-text reading.In addition, 10 publications were excluded (Table 1) because 2 were case reports with no follow-up period, 3 did not mention the number of implants placed, and 5 studies were laboratory comparisons using finite elements and superimposed models with no clinical evidence (Figure 2).With the final reading of the articles by the two researchers (JCN and GB), 3 additional articles were included in the review as a result of reading the bibliographical references in the publications analyzed (ICL classification, systematic review, comparative clinical study).Thus, 41 studies were included in this systematic review, according to the PRISMA flowchart illustrated in Figure 2. The recommended methodology was used for the metaanalysis [16].The CONSORT checklist, in Table 2, was used for the qualitative assessment of the studies [17].
The quality of the articles published by CONSORT criteria was assessed as Poor when they had a score of less than 10.50, Average when they had a score between 10.50 and 21, Good when they had a score between 22 and 31.50, and Excellent when they scored between 31.50 and 43 of the items analyzed [18].Of the articles selected, 75% met the criteria between 16 and 21 (15 articles) and were therefore classified as average quality, while 25% scored between 22 and 31.50 (five articles) considered as good quality.It is important to point out that the fulfillment of the constant items showed that the risk of bias of the articles met the requirements for the strategy to be between average and good quality.

Results
The characteristics of the thirteen retrospective and six prospective studies evaluated are described in Table 3.A total of 2047 patients were described, except for the study [18] which did not provide information on the number of participants, with a total of 7319 restorations evaluated.
Table 2. Checklist for analyzing the methodology and risk of bias of the studies analyzed based on CONSORT (Consolidated Standards of Reporting Trial).The cross-marked studies (×) show compliance with the parameter assessed; blank cells represent non-compliance with the item analyzed.The omitted parameters 1b, 2b, 4a, 4b, 5, 12a, 14a, 14b, 19, 20, 21, and 22 shows compliance in all studies analyzed.
Various studies also recorded the time elapsed between the placement of the dental prosthesis and the interproximal opening space between the implant-supported prothesis and the adjacent tooth, which ranged from three months [32,33] to more than five years [7,24] or even ten years [19,27,35] or more, after installation [8, 14,19].A progressive increase in this opened space was observed over the time between installation and detection of the alteration [26,34].Food Impaction (FI) is known as a condition that influences and changes the gingival tissues.No gingival modifications were found in 12 studies [1,8,15,23,[31][32][33][34].Of the seven studies in which gingival conditions varied, six found gingival alterations [14,19,22,25,36], and one study found bone alterations [7].In the meta-analysis, periodontal and periimplant conditions were shown in 7 publications as alterations ranging from mucositis to loss of bone insertion, with biological variations of 60% (confidence interval 0.45 to 0.73) and heterogeneity: I 2 = 99%, τ 2 = 0.63, p < 0.01.In the other 12 publications, which reported no biological variations, a value of 45% was obtained (confidence interval 0.33 to 0.59) and heterogeneity: I 2 = 96%, τ 2 = 0.83, p < 0.01 (Figure 7).

Figure 7.
Forest plots: Analysis of subgroups in terms of loss of contact points and the occurrence of biological changes or no biological changes, subdividing the studies into two subgroups and demonstrating the proximity of both to the graph's central diamond.The green diamond and solid line represent the results of all studies together, while the red diamonds and dotted lines represent each group's results [7,15,19,22,30,31,34], [1,8,14,[23][24][25][26][27][28][29]32,33].

Discussion
Evaluating the periodontal and peri-implant consequences of ICL is important once it can be highly correlated with multiple aspects, such as the location of the prosthesis, type of retention, number of elements, time elapsed, and opening of interproximal space on periodontal health area.
In the present analysis, for the occurrence of ICL regarding location and type of retention, no changes were found for clinical effects in the region and for trigger changes in the periodontal or peri-implant area or even for the patient's general comfort [14,19,31].

Evaluation of Interproximal Contact Loss (ICL)
The assessment of ICL was carried out using different assessment tools, which prevented the authors from carrying out the meta-analysis.The methods varied from dental flossing, X-ray image evaluation, matrix strips of different dimensions, and clinical evaluation by routine examinations [3] or according to patients' reports [20,21].Large discrepancies in the diagnosis (between 13% and 66%) were found in some studies [1,28], which Forest plots: Analysis of subgroups in terms of loss of contact points and the occurrence of biological changes or no biological changes, subdividing the studies into two subgroups and demonstrating the proximity of both to the graph's central diamond.The green diamond and solid line represent the results of all studies together, while the red diamonds and dotted lines represent each group's results [1,7,8,14,15,19,[22][23][24][25][26][27][28][29][30][31][32][33][34].

Discussion
Evaluating the periodontal and peri-implant consequences of ICL is important once it can be highly correlated with multiple aspects, such as the location of the prosthesis, type of retention, number of elements, time elapsed, and opening of interproximal space on periodontal health area.
In the present analysis, for the occurrence of ICL regarding location and type of retention, no changes were found for clinical effects in the region and for trigger changes in the periodontal or peri-implant area or even for the patient's general comfort [14,19,31].

Evaluation of Interproximal Contact Loss (ICL)
The assessment of ICL was carried out using different assessment tools, which prevented the authors from carrying out the meta-analysis.The methods varied from dental flossing, X-ray image evaluation, matrix strips of different dimensions, and clinical evaluation by routine examinations [3] or according to patients' reports [20,21].Large discrepancies in the diagnosis (between 13% and 66%) were found in some studies [1,28], which can be explained by the lack of protocol for the analysis of resistance to the passage of dental floss of different thicknesses (50 to 70 µm) as used as the main tool for ICL detection [10,18,20,22,28,37].Other studies qualified passage with minimal or no resistance to passage [7,8]; other studies scored this dental floss resistance with numbers 0, 1, 2, 3 [24,30,33] or with the letters a, b, and c [31,32].
The ICL was also measured using metallic strips [23,26,27,34,38] with a thickness variation of 0.1 to 0.5 mm as a tool to better measure the interproximal mesial-distal (M-D) space distance; an apical-coronal [20] metallic wire of different thicknesses was also used [18].Dental floss was used to detect ICL and, at the same time, used to overlay the scanned models.This methodology was important to assess changes that occurred not only in the mesiodistal direction but also in other directions [3].
In the X-ray image evaluation, the measurement was carried out by checking the contact between the dental crowns and also in the follow-up after corrections.This tool assessed the bone measurement, which was taken from the implant shoulder (platform) to the bone crest closest to the implant, without detailing whether the implants were Bone Level or Tissue Level [1,14,15,19,37,39,40].It is important to highlight the distortions that radiographic image evaluations can generate due to the difficulty of calibration and measuring the same position when repeating the technique.
Visual clinical assessment was carried out in all analyzed publications.The wide variation in ICL assessment methods may explain the discrepancies found in 13% [1], 23% [41], 65% [23], or even 78% in one reported trial [18].The importance of establishing a standardized methodology for evaluating ICL is highlighted so that the results are more faithful and, possibly, have a smaller margin of distortion.It is also essential to perform some recommendations to the patient on the possibility of ICL occurrence, the clinical signs, and the need to return to the dentist for evaluation, which must be part of the guidelines and good practices in the prosthetic rehabilitations and delivery protocol.

Food Impaction (FI)
FI was found in all studies where ICL was evaluated, with confirmation of FI reported by the patients between 32% and 56% [20] of the studied populations [14,18,22,37].However, in the studies in which there was no FI reported by the patients [7], gingival changes were still observed, such as mucositis and peri-implant changes [8, 15,19,22,42].The importance of monitoring these changes is reiterated, and if the change occurs, it must be monitored according to the loss of supporting tissue; observation may be necessary [11] in case of limited tissue loss or clinical return for evaluation and correction of cases of peri-implant disease [11,38,43] (Figure 1b).
If changes occur without loss of supporting tissue, this should only be monitored [11].The patient must be warned of the possibility of FI occurrence and the need for periodic clinical monitoring in order to evaluate and adjust where cavities and peri-implant disease may be present [10,11,38,43].It is still well observed that AI was the main cause in the development of cavities on the distal surfaces of the teeth when they were adjacent to the implant and highlights the need for information about this fact to the patient to justify strategies to prevent the development of carious lesions, such as fluorides, adequate brushing, flossing and the use of silver diamine fluoride [44].
In a finite element study, it was detected that the correct anatomical contour of the interproximal surface contact contributed to better load distribution on the adjacent tooth and the implanted-supported dental crown [4].The mesial region of the prosthesis over the implant was the most affected by the ICL [7,8,14,15,18,20,22,24,27,28,30,34]; however, a high variation was detected, from 10% [34] to 78% [14].
Regarding the location of the implant in the dental arch, ICL occurred in the maxilla from 14% [19] to 30% [8], but it was more frequently detected in the posterior region of the mandible, which ranged from 15% [1] to 54% [20].It should be noted that in some publications, only the posterior region (P) of the mandible was analyzed [33,34].In other studies, there was no distinction regarding the intra-oral region (A or P).Some studies that analyzed [1,14,30] the AP subgroups and those that [22,24,25,29] were carried out in the P subgroup were outside the diamond confidence limit and stood out.
Occlusion seems to be fundamental for the stability of the interproximal surface contact [1,3,5,9,10,14,18], highlighting the anterior force component [10,19,27] and the occlusion distribution with the antagonist arch [7,10,24,26].An important finding was that the ICL between single or multiple prostheses, when adjacent to the natural tooth, varied between 16% and 75% [15,20].The studies highlighted the use of single retention [18,32] and those that did not distinguish whether the prostheses were single or multiple [1,18,24,30] but were outside the confidence interval of 0.35 to 0.69.
The time elapsed between the placement of the prosthesis and the detection of the interproximal space was very variable, being the earliest at 3 months [18,22] and may increase over time [7,8].On the other hand, it can be activated later, 8 or more years after the prosthesis connection [19].No differences were found for the type of retention, as prostheses retained by cement or by screws [29], and the occurrence of ICL.However, it was also reported that for those retained by screws, they were easier to maintain; it was easier to reestablish the correct interproximal contact, and they could be restored even with the prosthesis or the adjacent tooth [18,28,37].
Patient age was another topic analyzed in this systematic review [36,38] and highlighted as a determining factor for the establishment of ICL, except in [23], which relates the loss of attachment to be aggravated with advancing age and increased tooth mobility.Some studies analyzed the influence of gender and found that males had a higher prevalence of ICL [1,5,6,15].

Clinical Effects of Interproximal Contact Loss (ICL)
The main effect reported in ICL was FI; some publications directly stated a relationship.It was detected prior to the evaluation by some patients due to discomfort, and in some cases by filling a questionnaire relating to pain symptoms affecting quality of life [15,19,37].However, other trial results [20,22,23] showed little perception of FI noted by the patient.
The consequences of FI on the gingival tissues near the tooth or implant-supported prosthesis are not very clear in the literature [1,8,14,26,32,37].However, inflammation of the periodontal and peri-implant regions was detected by probing the periodontal groove and peri-implant [7,18,19,22,24,26,30,34].Patients who presented bone loss between the natural tooth and the implanted-supported prosthesis may have an increased risk for ICL [42].In this meta-analysis, the group with biological changes resulted in 60% and a range from 45% to 73% with a randomized effect of I 2 = 99%, τ 2 = 0.63, p < 0.01.However, it should be highlighted that the study [14] was outside the edge of the diamond on the chart.For the group where no biological variations occurred, the meta-analysis resulted in 45% and a range of 33% to 59% with a randomized effect of I 2 = 99%, τ 2 = 0.84, p < 0.01, but it should be noted that the study [1] was left out of the diamond graph in Figure 7.
It is important to educate the patient regarding the need for correct hygiene with dental floss [33].Some publications have shown the development of carious lesions in the natural tooth adjacent to the prosthesis over the implant [2,6,8,10,14,19,22,40,44].Although ICL was more prevalent in the mesial region of the implant prosthesis than in the distal region, there was an important variation between the literature analyzed as the divergence of results was relevant in the mesial region from 4.1% [39] to 85.4% [15] and distally with a variation of 2.3% [1] to 38.9% [34].
The diversity of interproximal space/contact assessment tools and methods may explain some discrepancies regarding the type of prosthesis retention (screwed or cemented) and as not being related to the ICL occurrence [1,5,10,15,20,23].There has not yet been confirmation of the influence of the type of retention on the occurrence of ICL [14,18,19,22,25,26,29,31,32,35,36,41].The diversity in contact point assessment methods and the discrepancy regarding the type of prosthesis retention may explain the lack of correlation between the types of retention and the ICL, according to the meta-analysis result of 55%, confidence interval from 0.40 to 0.68 (p < 0.01); in contrast to only three studies that mention screw-retained prostheses [8, 28,38] with a range of 37% (0.26 to 0.50) p < 0.01.Three publications stand out in that they do not mention the type of retention applied [7,26,27].
Important arguments were made in [33] so that the retention is screwed, aiming to facilitate the ICL reconstruction, either with light-cured resins [11] or with ceramic additions to the prostheses [10] or, even, through the reconstruction of the adjacent tooth with resin or with inlays/onlays [9].Correct reconstruction of the interproximal surface contact is essential for reestablishing periodontium homeostasis, for patient comfort, and for the rehabilitation performance over time (Figure 8).
The diversity of interproximal space/contact assessment tools and methods may explain some discrepancies regarding the type of prosthesis retention (screwed or cemented) and as not being related to the ICL occurrence [1,5,10,15,20,23].There has not yet been confirmation of the influence of the type of retention on the occurrence of ICL [14,18,19,22,25,26,29,31,32,35,36,41].The diversity in contact point assessment methods and the discrepancy regarding the type of prosthesis retention may explain the lack of correlation between the types of retention and the ICL, according to the meta-analysis result of 55%, confidence interval from 0.40 to 0.68 (p < 0.01); in contrast to only three studies that mention screw-retained prostheses [8, 28,38] with a range of 37% (0.26 to 0.50) p < 0.01.Three publications stand out in that they do not mention the type of retention applied [7,26,27].
Important arguments were made in [33] so that the retention is screwed, aiming to facilitate the ICL reconstruction, either with light-cured resins [11] or with ceramic additions to the prostheses [10] or, even, through the reconstruction of the adjacent tooth with resin or with inlays/onlays [9].Correct reconstruction of the interproximal surface contact is essential for reestablishing periodontium homeostasis, for patient comfort, and for the rehabilitation performance over time (Figure 8).An important finding reported [34] the impact of using an occlusal splint (2 mm thick) on the incidence of ICL.The study revealed that the group that did not utilize a containment drip had the highest incidence of ICL at 30%, whereas the group that used a containment drip saw a lower incidence of 15% during the study period.These results suggest promising avenues for future research in this area.
The occurrence of ICL in various locations in the oral cavity was analyzed, both in the maxilla and mandible, being mostly found in the mandible in the posterior region in the mesial portion of the prosthesis over the implant [35,36,38].However, in the metaanalysis, the P region with 47% (0.36 to 0.59) with p < 0.01 demonstrates three studies [31, 32,38] that escaped the confidence interval of the diamond chart (0.36 to 0.59).Furthermore, 55% (0.37 to 0.71) of the publications did not distinguish between Anterior and Posterior locations with p < 0.01, and four studies in this region [1,14,15,30] served outside the confidence interval (0.37 to 0.73) of the diamond chart.An important finding reported [34] the impact of using an occlusal splint (2 mm thick) on the incidence of ICL.The study revealed that the group that did not utilize a containment drip had the highest incidence of ICL at 30%, whereas the group that used a containment drip saw a lower incidence of 15% during the study period.These results suggest promising avenues for future research in this area.
The occurrence of ICL in various locations in the oral cavity was analyzed, both in the maxilla and mandible, being mostly found in the mandible in the posterior region in the mesial portion of the prosthesis over the implant [35,36,38].However, in the meta-analysis, the P region with 47% (0.36 to 0.59) with p < 0.01 demonstrates three studies [31, 32,38] that escaped the confidence interval of the diamond chart (0.36 to 0.59).Furthermore, 55% (0.37 to 0.71) of the publications did not distinguish between Anterior and Posterior locations with p < 0.01, and four studies in this region [1,14,15,30] served outside the confidence interval (0.37 to 0.73) of the diamond chart.
Several hypotheses have been discussed in the literature regarding the ICL occurrence being the most accepted, the passive eruption of the teeth [3,7,10], the continuous growth of the face [10,19], and the anterior force component [1,10,26], as well as the excess/direction of the load that can cause mesialization and/or movement of the tooth [31].Those considerations should be observed when monitoring each of the cases.To reduce the possibility of ICL, reconstruction and correct adjustment of contact points are essential for adequate occlusal load distribution to the tooth and bone tissue, as well as to the implant and underlying region, promoting homeostasis in those fields [2,6,24,37] and minimizing the occurrence of ICL [4,5], a greater adjustment or tightening of the surface contact is also recommended [33].Tooth wear surfaces due to passive eruption must be observed, and the neutralization of the anterior load component should be monitorized [10].
Due to the high prevalence of ICL, it is always recommended that implant prostheses be retained with screws to facilitate the reconstruction of contact in the region, whether by adding them to the tooth or to the prosthesis.Some authors [27] also suggested the inclusion in recommendations to patients that ICL is a predictable event that could occur at any time, with the need for monitoring and/or reconstruction.

Conclusions
This review assessed the clinical effects of Interproximal Contact Loss (ICL), the impact of the type of prosthesis retention, the number of prosthetic elements, and the anterior or posterior positioning of the rehabilitation.Additionally, the primary assessment tools described in clinical trials for detecting ICL and measuring interproximal space/contact over time were examined.
ICL occurrence was found to be a complex phenomenon influenced by multiple factors.Changes were most frequently observed on the mesial surface of the implant prosthesis, particularly in the posterior mandibular region.In the anterior region, the earliest instances were detected at 3 months.Subsequent ICL frequency tendency increased over time.ICL played a role in food impaction, leading to tissue changes (mucositis) that could progress to peri-implantitis, making it a risk factor for gingival and periodontal conditions.
Factors such as continuous tooth movement, anterior load distribution, and occlusal contact were identified as significant contributors to ICL, particularly on the mesial surface of implant-supported prostheses.While the use of an occlusal splint may not prevent ICL, it can help mitigate some of the clinical effects when ICL occurs.
Due to the multiplicity of assessment tools and methods, new calibration and protocols are required to develop and standardize clinical and research evaluation of ICL, allowing reliable and early detection of interproximal space.

Figure 1 .
Figure 1.(a) Relationship between implant in position 35 and contact between implant prosthesis and adjacent natural teeth.The healthy peri-implant and periodontal regions are shown in the black circle.(b) Implant in position 35, interproximal contact loss (ICL) between prosthesis on implant and natural tooth with food impaction, caries in 34, and loss of gingival volume are visible.

Figure 1 .
Figure 1.(a) Relationship between implant in position 35 and contact between implant prosthesis and adjacent natural teeth.The healthy peri-implant and periodontal regions are shown in the black circle.(b) Implant in position 35, interproximal contact loss (ICL) between prosthesis on implant and natural tooth with food impaction, caries in 34, and loss of gingival volume are visible.

Figure 2 .
Figure 2. PRISMA flowchart of the strategy used in the literature search.

Figure 2 .
Figure 2. PRISMA flowchart of the strategy used in the literature search.

Figure 4 .
Figure 4. Forest plots: Analysis of loss of contact point according to the number of prostheses per implant.This is demonstrated by the graph in sub-division by Single/multiple (S-M) in 9 studies and Single (S) Prostheses in another 10 studies.The green diamond and solid line represent the results of all studies together, while the red diamonds and dotted lines represent each group's results [1,7,8,14,15,19,22-34].

Figure 5 .
Figure 5. Forest plots: Analysis of loss of contact point according to the location of the prostheses.Demonstrated by graphs in subgroups by Antero-Posterior (AP) location in 9 studies and Posterior (P) in another 10 studies.The green diamond and solid line represent the results of all studies together, while the red diamonds and dotted lines represent each group's results [1,7,8,14,15,19,22-34].

Figure 8 .
Figure 8. Implant in position 35, removal of caries, and reconstruction of interproximal contact loss (ICL) between prosthesis on implant adjacent to natural tooth.Note the deficient periodontal space.

Figure 8 .
Figure 8. Implant in position 35, removal of caries, and reconstruction of interproximal contact loss (ICL) between prosthesis on implant adjacent to natural tooth.Note the deficient periodontal space.

Table 1 .
Exclusion criteria and number of studies excluded after reading the manuscripts.

Table 3 .
A description of the studies included in the systematic review and meta-analysis.