SuperPro Designer ® , User-Oriented Software Used for Analyzing the Techno-Economic Feasibility of Electrical Energy Generation from Sugarcane Vinasse in Colombia

: SuperPro Designer ® is a process simulator software used for analyzing the techno-economic feasibility of large-scale bioprocesses. Its predetermined built-in features allow for easy implementation by non-experts, but a lack of examples limits its appropriation. This study aims to validate the implementation of SuperPro Designer ® by non-experts for the techno-economic analysis of anaerobic digestion in Colombia, using vinasse as feedstock. These results demonstrate the ﬁnancial feasibility of such a process when a processing ﬂow rate of 25 m 3 / h is ensured. Additionally, this study validates the manageability of the tool for assessing the economic feasibility of a technology, a key practice during technology development regardless of the area of expertise.


Introduction
The implementation of large-scale strategies for waste management, such as anaerobic digestion (AD), requires economic evaluation since the establishment of these approaches largely depends on the technical and geopolitical conditions of the specific location. Therefore, techno-economic analyses (TEA) are essential for determining the feasibility of a project, and they must be adapted to the location of interest. TEA commonly combine process modeling, engineering design, and economic evaluation, by assessing the economic viability of a process and identifying sensible and cost-risk points during the development and implementation stages [1][2][3][4].
Excel ® and ASPEN Plus ® are the most commonly used software products for TEA during the development stages associated with process operation and optimization. Excel ® is widely used due to its availability and easy access. Process calculations are performed with spreadsheets, which limits the analysis to a small number of units. This is because the formulas to calculate thetotal cost categories for a TEA are commonly scattered across multiple sheets, which makes it difficult for the user to follow. Spreadsheet models are based on pre-existing templates for specific scenarios, which require a later ranges from 10 to 13 L per liter of ethanol [26,27]. In Colombia, annexed distilleries have reduced vinasse volume by a series of recirculation and energy-intensive evaporation steps to increase its solid content fivefold, from~6% to~30% total solids (TS) [28][29][30][31][32]. Due to its composition, vinasse has being used in composting processes and direct land application via fertigation, partially satisfying water needs. Depending on soil physicochemical characteristics, these practices can result in hydro-chemical changes altering soil and water dynamics, polluting these environments in the long term [28,33]. Alternative strategies to manage and even valorize this byproduct are currently under evaluation. The anaerobic treatment of biomass has been recognized as one of the most attractive strategies for the development of alternative energy sources since it converts more than 50% of the initial organic material into biogas, a renewable energy source, and digestate, a high-quality agricultural fertilizer [34][35][36]. Due to the potential of AD to valorize sugarcane-based vinasse and the number of TEA evaluations using Excel ® and Aspen Plus ® performed in other countries (e.g., Brazil), we aimed to validate the easy implementation of SuperPro Designer ® using many of its built-in features to evaluate the techno-economic feasibility of an anaerobic digestion plant fed with sugarcane-based vinasse for electrical energy generation in Colombia.

Physicochemical Characterization of Inoculum and Substrates
The starting inoculum was a digested sludge from the Cañaveralejo wastewater treatment plant (Cali, Colombia). Sugarcane-based vinasses were collected from six annexed distilleries located in the Cauca Valley region in Colombia, before and after evaporation steps when possible. Substrate sampling was carried out during different seasons, and the samples were stored at 4 • C until processing. The total solid (TS), volatile solids (VS), and humidity of all the vinasses and the starting sludge were measured by combustion and gravimetric methods according to the National Renewable Energy Laboratory (NREL) protocols (NREL/TP-510-42621). For each vinasse, a representative sample of 4 mL was used to measure the pH using a HI 2030 pH meter (Hanna Instruments, Italy). Carbon (C) and nitrogen (N) contents were determined via dry combustion for each substrate by an external service in the analytical laboratory at the International Center for Tropical Agriculture (CIAT).

Biomethane Potential (BMP) Analysis
The VDI 4630 technical guidelines were used to carry out the BMP analysis of the collected vinasses. The starting inoculum contained 8.33 g/L of TS and 3.25 g/L of VS. The experimental unit consisted of 30 batch bench-scale 1L reactors with a 60% working volume and constant agitation. Biogas generation was quantified using an inline flow meter, and the biogass composition was determined with a portable BIOGAS 5000 (Geotech, UK). A 1:6 VS-substrate % ratio/VS-inoculum % ratio and mesophilic conditions were used in the BMP analysis. The energy potential was estimated based on the resulting BMP using the following equation, assuming that 1 m 3 of biogas generated 11.70 kW/h [37] (Equation (1)

Statistical Analysis
The Mann-Whitney U test was used for statistical analysis using the SigmaPlot ® 13.0 software (Systat Software, Inc., Chicago, IL, USA), to assess the correlation between the % VS and BMP values, with p < 0.05.

Model Generation for Biogas Production: Simulation Description
An AD-plant-processing sugarcane-based vinasse for electrical energy generation was simulated using the SuperPro Designer software v9.5 (Intelligen, Inc., Scotch Plains, NJ, USA). A 15-year lifetime was assumed, including a one-year construction and start-up phase. A continuous process was set up with a 312 days/year operation time with 85% working capacity. Microsoft Excel ® 2010 was used to calculate the plant's cumulative cash flow. The stoichiometric conversion of the biotransformation process was fixed according to the BMP analysis.

Process Description
Bioreactors with a maximum volume of 5000 m 3 were defined with a 90% working vessel volume and 95 h residence time. The process model was assumed to be operated under continuous mode. The process was divided into three main steps: compression, burning, and expansion. First, the diluted vinasse was fed to a storage tank (P1/ST) to control a constant flow for the AD reactor (P2/AD). Then, the produced biogas was conducted to a gas compressor (P4/GC) to be burned (P5/GB), and the combustion gases were later used in gas expansion (P6/GE) to generate electricity. A flow splitter (P3/SP) returned 5% of the flow to a mixer (P0/MX) to be included in the diluted vinasse-fed stream. Some of the digestion product was recycled as inoculum. The non-recycled stream was fed to a centrifugal decanter (P7/CD) to obtain a sludge cake with 66% solid content, which yielded wastewater with a solid content of <0.5% (w/w). For the expansion step, an isentropic expansion was configured ( Figure 1). The process was modeled using SuperPro Designer ® , and the AD process parameters are summarized in Table 1. The combustion gas flow was defined as the revenue stream for the model, considering a selling price of 0.1 USD/kWh for the electric power produced, and the electrical energy generation was estimated using the built-in efficiency model in SuperPro Designer ® .

Process Description
Bioreactors with a maximum volume of 5000 m 3 were defined with a 90% working vessel volume and 95 h residence time. The process model was assumed to be operated under continuous mode. The process was divided into three main steps: compression, burning, and expansion. First, the diluted vinasse was fed to a storage tank (P1/ST) to control a constant flow for the AD reactor (P2/AD). Then, the produced biogas was conducted to a gas compressor (P4/GC) to be burned (P5/GB), and the combustion gases were later used in gas expansion (P6/GE) to generate electricity. A flow splitter (P3/SP) returned 5% of the flow to a mixer (P0/MX) to be included in the diluted vinasse-fed stream. Some of the digestion product was recycled as inoculum. The non-recycled stream was fed to a centrifugal decanter (P7/CD) to obtain a sludge cake with 66% solid content, which yielded wastewater with a solid content of <0.5% (w/w). For the expansion step, an isentropic expansion was configured ( Figure 1). The process was modeled using SuperPro Designer ® , and the AD process parameters are summarized in Table 1. The combustion gas flow was defined as the revenue stream for the model, considering a selling price of 0.1 USD/kWh for the electric power produced, and the electrical energy generation was estimated using the built-in efficiency model in SuperPro Designer ® .

Economic Analysis
The economic performance of the plant was measured by calculating the Fixed Capital Investment and Working Capital Costs ( Table 2). The equipment size (Table 3) and purchase costs were estimated using the design mode with data provided by a local supplier and the SuperPro Designer ® built-in cost model, respectively.

Economic Analysis
The economic performance of the plant was measured by calculating the Fixed Capital Investment and Working Capital Costs ( Table 2). The equipment size (Table 3) and purchase costs were estimated using the design mode with data provided by a local supplier and the SuperPro Designer ® built-in cost model, respectively. To extrapolate the equipment investment cost, the following cost rule was used: C O is the investment cost for a piece of equipment with Qo process capacity. The "a" value was 0.6. The model predicts new equipment costs depending upon the modeled capacity according to mass and energy balance restrictions.
OPEX (operational expenditure) was estimated as the monthly cost of labor, raw materials, utilities, waste treatment, and disposal when the sludge and liquid from the centrifugation (decanter) were considered emissions. CAPEX (capital expenditure) was estimated in USD, and the annual OPEX breakdown was calculated as a function of the processed mass (kg) of sugarcane-based vinasse.

Sensibility Analysis
A sensibility analysis was carried out using SuperPro Designer ® . The analysis considered vinasse processing flow rates ranging from 5.0 to 125.0 kg/h.

Profitability Analysis
The following variables were used to determine the economic profitability of the process: vinasse processing flow rates ranging from 5.0 to 125.0 kg/h and cost/revenue of byproducts (digestate) ranging from −0.001 to +0.009 USD/kg, considering negative values as the costs of disposal, while positive values corresponded to revenues from sales. The gross margin, net present value (NPV) (Equation (2)), internal rate of return (IRR), payback time, and return on investment (ROI) (Equation (3)) were estimated using the SuperPro Designer ® software. The profit tax used for the Colombian scenario was 16.5%.
where t is the lifetime in years, C t is the net cash flow during period t, Co is the initial investment, and d is the discount rate (3% in this case). The IRR is the discount rate at which the NPV value is zero. IRR is commonly used to indicate the efficiency or yield of an investment. The payback time is the time required to recoup the investment cost, while the ROI describes the rate of the cash return.

Vinasse Characterization and BMP Analysis
All the sugarcane-based vinasses produced in the geographical valley of the Cauca River were characterized based on their physicochemical characteristics (Table 4). All the samples had a low pH ranging from 4.01 to 5.04, while the % VS ranged from 1.95 ± 1 × 10 −5 % to 13.78 ± 1 × 10 −4 % for the diluted vinasses and 14.88 ± 4 × 10 −4 % to 32.20 ± 0.001% for the concentrated ones. The % VS and % C are directly related to the organic matter content of each substrate. According to the strategies and efficiencies of the evaporation systems present in each mill, these values tended to vary. The C/N ratios, which reflect the nutrient levels of substrates, of the diluted vinasses were within the ranges 4.61-8.24 and 9.49-18.12 for concentrated vinasses. Organic matter added to a digestion system promotes the growth of anaerobic bacteria and biogas production, making anaerobic digestion systems sensitive to this relationship. Some studies indicate that the optimal range of the C/N ratio for a fermentation process for methane production is between 25 and 30 [38]. The BMP is used to quantify the maximum amount of methane that can be produced during the AD of organic substrates [39][40][41]. It also provides information on degradation kinetics and potential inhibitors [42]. Techno-economic assessments of AD plants depend on different parameters including the rate of methane production from the organic materials to be digested [43][44][45][46]. The amount of methane produced also has a significant influence on the design of the downstream processing of the produced biogas. It influences the choice of cogeneration to produce electricity and heat, as well as the number and capacity required. It also influences the design and quantity of biogas storage and purification systems [45].
A test was performed to determine whether there was a difference between mills, to establish if the evaporation process affected the biomethane production yield, and to define the biomethane production yield value to use in the simulations to perform a global analysis rather than a Sugar Mill-specific analysis. The results shown in Figure 2 indicate that there was not a statistically significant difference (p = 0.437) between the biogas yields among mills when the % VS values were similar. By contrast, there was a statistically significant difference (p = 0.019) between the concentrations of solids and biogas production. Additionally, the results suggest that biogas production is inversely correlated with the % VS; the higher the concentration, the lower the biogas production. This relationship indicates that the concentration process not only increases the % VS but may also increase the quantity of inhibitory compounds or reduce the biodegradability of the organic matter due to the heating process. In 2013, Budiyono and collaborators [47] determined the effect of different vinasse/water ratios on biogas yield. They concluded that the 1:3 ratio (TS, 7.015 ± 0.007%) yielded the highest rate of biogas production; this study suggests that the presence of inhibitory compounds explains the obtained results, rather than an effect on biodegradability. methane produced also has a significant influence on the design of the downstream processing of the produced biogas. It influences the choice of cogeneration to produce electricity and heat, as well as the number and capacity required. It also influences the design and quantity of biogas storage and purification systems [45].
A test was performed to determine whether there was a difference between mills, to establish if the evaporation process affected the biomethane production yield, and to define the biomethane production yield value to use in the simulations to perform a global analysis rather than a Sugar Millspecific analysis. The results shown in Figure 2 indicate that there was not a statistically significant difference (p = 0.437) between the biogas yields among mills when the % VS values were similar. By contrast, there was a statistically significant difference (p = 0.019) between the concentrations of solids and biogas production. Additionally, the results suggest that biogas production is inversely correlated with the % VS; the higher the concentration, the lower the biogas production. This relationship indicates that the concentration process not only increases the % VS but may also increase the quantity of inhibitory compounds or reduce the biodegradability of the organic matter due to the heating process. In 2013, Budiyono and collaborators [47] determined the effect of different vinasse/water ratios on biogas yield. They concluded that the 1:3 ratio (TS, 7.015 ± 0.007%) yielded the highest rate of biogas production; this study suggests that the presence of inhibitory compounds explains the obtained results, rather than an effect on biodegradability. This is the first analysis of the correlation between % VS and biomethane production yield carried out with raw sugarcane-based vinasses generated before (diluted) and after (concentrated) the evaporation steps in all annexed distilleries in Colombia. According to the data, the vinasse concentration, not the source, impacts biogas production. As such, larger feedstock volumes result in higher investment costs [48], which affects the economic feasibility of a project.

TEA Evaluation and Sensitivity Analysis
The TEA of a vinasse-fed AD plant used for the generation of electricity was performed, taking into account the reported correlation between the % VS and the biogas yield. As reported previously, lower concentrations of VS generate greater biogas potential. Therefore, diluted vinasse, with 6.9% of VS (w/w), was used to perform the simulations with SuperPro Designer ® . In the model, the % VS This is the first analysis of the correlation between % VS and biomethane production yield carried out with raw sugarcane-based vinasses generated before (diluted) and after (concentrated) the evaporation steps in all annexed distilleries in Colombia. According to the data, the vinasse concentration, not the source, impacts biogas production. As such, larger feedstock volumes result in higher investment costs [48], which affects the economic feasibility of a project.

TEA Evaluation and Sensitivity Analysis
The TEA of a vinasse-fed AD plant used for the generation of electricity was performed, taking into account the reported correlation between the % VS and the biogas yield. As reported previously, lower concentrations of VS generate greater biogas potential. Therefore, diluted vinasse, with 6.9% of VS (w/w), was used to perform the simulations with SuperPro Designer ® . In the model, the % VS was assumed as the reaction-limiting factor to propose the following stoichiometric equation for biogas production expressed in mass: 1 g VS + 0.19 g H 2 O → 0.64 g CO 2 + 0.008 g H 2 S + 0.0009 g H 2 + 0.46 g CH 4 + 0.05 g N 2 + 0.03 g sludge (4) The simulation was used to assess the main factors that influenced the economic performance of the process through a sensitivity analysis, and determine under which conditions this process was economically feasible.
Siddique and collaborators [49] indicated that an optimum flow rate during the AD process is a critical point for the design and management of an AD plant. The processing flow rate affected two important parameters: biogas and methane yield and energy assessment. Thus, the amount of produced biomethane has a strong impact on the energy balance and economic feasibility of an AD plant [50]. Other researchers have reported additional factors that affect the economic feasibility of implementing an AD plant, such as energy capacity and operating cost, which varies according to the country's economic conditions [44,[49][50][51].
The installed ethanol capacity in an annexed Colombian distillery ranges between 100,000 and 300,000 L/day, which corresponds to a vinasse production rate of 5-12.5 m 3 /h depending on the number of evaporation steps. Figure 3 shows the effect of the vinasse processing flow rate on the biogas generation flow and potential electric energy generation capacity of an annexed distillery in Colombia. The vinasse processing flow rate has a linear relationship with biogas flow and power generation, which indicates a direct association between biogas production and power generation. Higher vinasse processing flow rates allow for greater biogas production and, consequently, a higher power generation capacity. These findings support previously reported data [52,53]. Using the maximum vinasse generation capacity reported for each annexed distillery in Colombia, the largest can produce 12.5 m 3 /h, which, in turn, generates~6000 m 3 of biogas/h and 20.4 MW of electrical energy. was assumed as the reaction-limiting factor to propose the following stoichiometric equation for biogas production expressed in mass: 1 g VS + 0.19 g H2O → 0.64 g CO2 + 0.008 g H2S + 0.0009 g H2 + 0.46 g CH4 + 0.05 g N2 + 0.03 g sludge (4) The simulation was used to assess the main factors that influenced the economic performance of the process through a sensitivity analysis, and determine under which conditions this process was economically feasible.
Siddique and collaborators [49] indicated that an optimum flow rate during the AD process is a critical point for the design and management of an AD plant. The processing flow rate affected two important parameters: biogas and methane yield and energy assessment. Thus, the amount of produced biomethane has a strong impact on the energy balance and economic feasibility of an AD plant [50]. Other researchers have reported additional factors that affect the economic feasibility of implementing an AD plant, such as energy capacity and operating cost, which varies according to the country's economic conditions [44,[49][50][51].
The installed ethanol capacity in an annexed Colombian distillery ranges between 100,000 and 300,000 L/day, which corresponds to a vinasse production rate of 5-12.5 m 3 /h depending on the number of evaporation steps. Figure 3 shows the effect of the vinasse processing flow rate on the biogas generation flow and potential electric energy generation capacity of an annexed distillery in Colombia. The vinasse processing flow rate has a linear relationship with biogas flow and power generation, which indicates a direct association between biogas production and power generation. Higher vinasse processing flow rates allow for greater biogas production and, consequently, a higher power generation capacity. These findings support previously reported data [52,53]. Using the maximum vinasse generation capacity reported for each annexed distillery in Colombia, the largest can produce 12.5 m 3 /h, which, in turn, generates ~6000 m 3 of biogas/h and 20.4 MW of electrical energy. To determine the energy-recovery capacity of a sugar mill depending on the vinasse processing flow rate, the energy yield of the process was plotted as a function of the inlet vinasse flow rate (Figure 4). At low vinasse processing flow rates, there is a sharp increase in energy yield that stabilizes at flow rates >20 m 3 /h. The results suggest that 3.4 kW/m 3 of biogas will be the maximum electrical energy yield. To determine the energy-recovery capacity of a sugar mill depending on the vinasse processing flow rate, the energy yield of the process was plotted as a function of the inlet vinasse flow rate (Figure 4). At low vinasse processing flow rates, there is a sharp increase in energy yield that stabilizes at flow rates >20 m 3 /h. The results suggest that 3.4 kW/m 3 of biogas will be the maximum electrical energy yield.   The observed results align with previous studies carried out using Excel ® and Aspen Plus ® , where higher processing flow rates resulted in higher electrical generation capacity, with an     The observed results align with previous studies carried out using Excel ® and Aspen Plus ® , where higher processing flow rates resulted in higher electrical generation capacity, with an equivalent increase in CAPEX but not in OPEX [24,52,54]. To understand the differential increases in The observed results align with previous studies carried out using Excel ® and Aspen Plus ® , where higher processing flow rates resulted in higher electrical generation capacity, with an equivalent increase in CAPEX but not in OPEX [24,52,54]. To understand the differential increases in OPEX and CAPEX, Labor and Laboratory/QC/QA expenses as functions of the vinasse processing flow rate are shown in Figure 6.  Following previous studies carried out with Excel ® and Aspen Plus ® , the CAPEX and OPEX of an AD plant mainly depend on the technological approaches considered and the processing flow rate employed [24,55]. Our findings indicate that the vinasse processing flow rate is a critical factor that dictates the economic feasibility of a vinasse-fed AD plant for the generation of electrical energy from biogas. Additionally, they support the need to assess alternative uses for the biogas, as well as to incorporate additional unit operations associated with digestate utilization to improve the economics of vinasse-fed AD plants with low flow rates [56].

Economic Assessment
One advantage of SuperPro Designer ® compared to Aspen Plus ® and Excel ® is its costing feature, which is useful for calculating equipment costs and plant-operating costs once the simulation is completed. This feature may be further adjusted to local prices if necessary. The estimation of equipment costs was performed based on the mass and energy balances generated by the simulation. According to the model, the total equipment costs for a vinasse-fed AD-plant (5000 m 3 ) were 1,034,524.57 USD. It is worth highlighting that previous techno-economic analyses with Excel ® and Aspen Plus ® for sugarcane-based vinasse processing through AD did not report equipment cost estimations [54,57].
An additional advantage of SuperPro Designer ® is its economic evaluator, which was specifically developed for bioprocesses. This tool is designed to determine the dynamic behavior of various economic parameters under different scenarios. This tool was employed to assess the impacts of the vinasse processing flow rate and the costs/revenues of the digestate on the feasibility of a vinasse-fed AD plant. The vinasse processing flow rate exerted a big impact on the CAPEX ( Figure 5) and the fixed labor-dependent and laboratory/QA/QC-related costs (Figure 6), as previously shown. On the other hand, digestate, a mixture of microbial biomass and undigested material that is produced in large volumes after AD [58], considerably affects the economic feasibility of AD due to the potential costs of its disposal or treatment. However, it may be used for fertilization [59]. Thus, a range of Following previous studies carried out with Excel ® and Aspen Plus ® , the CAPEX and OPEX of an AD plant mainly depend on the technological approaches considered and the processing flow rate employed [24,55]. Our findings indicate that the vinasse processing flow rate is a critical factor that dictates the economic feasibility of a vinasse-fed AD plant for the generation of electrical energy from biogas. Additionally, they support the need to assess alternative uses for the biogas, as well as to incorporate additional unit operations associated with digestate utilization to improve the economics of vinasse-fed AD plants with low flow rates [56].

Economic Assessment
One advantage of SuperPro Designer ® compared to Aspen Plus ® and Excel ® is its costing feature, which is useful for calculating equipment costs and plant-operating costs once the simulation is completed. This feature may be further adjusted to local prices if necessary. The estimation of equipment costs was performed based on the mass and energy balances generated by the simulation. According to the model, the total equipment costs for a vinasse-fed AD-plant (5000 m 3 ) were 1,034,524.57 USD. It is worth highlighting that previous techno-economic analyses with Excel ® and Aspen Plus ® for sugarcane-based vinasse processing through AD did not report equipment cost estimations [54,57].
An additional advantage of SuperPro Designer ® is its economic evaluator, which was specifically developed for bioprocesses. This tool is designed to determine the dynamic behavior of various economic parameters under different scenarios. This tool was employed to assess the impacts of the vinasse processing flow rate and the costs/revenues of the digestate on the feasibility of a vinasse-fed AD plant. The vinasse processing flow rate exerted a big impact on the CAPEX ( Figure 5) and the fixed labor-dependent and laboratory/QA/QC-related costs (Figure 6), as previously shown. On the other hand, digestate, a mixture of microbial biomass and undigested material that is produced in large volumes after AD [58], considerably affects the economic feasibility of AD due to the potential costs of its disposal or treatment. However, it may be used for fertilization [59]. Thus, a range of values was used to represent the costs of disposal when negative and potential revenues when positive (Figure 7).
The gross margin ( Figure 7A), ROI ( Figure 7B), payback time ( Figure 7C), NPV ( Figure 7D), and IRR (Figure 8) as functions of the vinasse processing flow rate and cost/revenue of the digestate were used to assess the feasibility of an AD plant fed with sugarcane-based vinasse for electrical energy generation. According to the gross margin indicator (Figure 7A), the process is feasible using a vinasse processing flow rate higher than 60 m 3 /h and a return of at least 2.2% for the digestate to achieve a margin between 50 and 85% ( Figure 7A). High flow rates are also needed (80 to 125 m 3 /h) to achieve a reasonable payback time of 6-8 years ( Figure 7C). By contrast, the ROI ( Figure 7B), NPV ( Figure 7D), and IRR ( Figure 8) parameters suggest that feasibility is achieved at considerably lower flow rates (20,17, and 25 m 3 /h, respectively) regardless of the cost of digestate disposal. values was used to represent the costs of disposal when negative and potential revenues when positive (Figure 7). The gross margin ( Figure 7A), ROI ( Figure 7B), payback time ( Figure 7C), NPV ( Figure 7D), and IRR ( Figure 8) as functions of the vinasse processing flow rate and cost/revenue of the digestate were used to assess the feasibility of an AD plant fed with sugarcane-based vinasse for electrical energy generation. According to the gross margin indicator (Figure 7A), the process is feasible using a vinasse processing flow rate higher than 60 m 3 /h and a return of at least 2.2% for the digestate to achieve a margin between 50 and 85% ( Figure 7A). High flow rates are also needed (80 to 125 m 3 /h) to achieve a reasonable payback time of 6-8 years ( Figure 7C). By contrast, the ROI ( Figure 7B  The economic assessment indicates that the financial feasibility of a vinasse-fed AD plant will largely depend on the vinasse processing capacity of the distillery. In countries such as Brazil, where the harvest period is ~8-10 months, the seasonal variability can have a large effect. In Colombia, the harvesting occurs throughout the year, which ensures a constant feedstock availability. The economic assessment indicates that the financial feasibility of a vinasse-fed AD plant will largely depend on the vinasse processing capacity of the distillery. In countries such as Brazil, where the harvest period is~8-10 months, the seasonal variability can have a large effect. In Colombia, the harvesting occurs throughout the year, which ensures a constant feedstock availability. The geographic valley of the Cauca River has six annexed distilleries with installed ethanol capacities between 100,000 and 400,000 L/day, corresponding to vinasse production rates between 6 and 12.5 m 3 /h. Their corresponding vinasse flow rates are as follows: Mill I-6 m 3 /h; Mill II-12.5 m 3 /h; Mill III-10 m 3 /h; Mill IV-12.5 m 3 /h; Mill V-5 m 3 /h; and Mill VI-10 m 3 /h. According to these results, none of the distilleries generate enough vinasse to implement a feasible vinasse-fed AD plant for electrical energy generation. However, based on the distilleries' geographical distribution ( Figure  9), an alternative to increasing vinasse processing flow rates is to join vinasse streams between neighboring mills, such as Mills I-IV; this strategy could yield a processing flow rate of 41 m 3 /h. Improving the economic feasibility of a vinasse-fed AD plant while maintaining lower flow rates can also be achieved by the following strategies: increasing the C/N ratio of the substrate; increasing its biomethane potential by incorporating unit operations to valorize alternative waste streams, namely, the carbon dioxide present in the biogas, which reduces its calorific value (and through carboncapture technologies, it is possible to exploit it or synthesize high-value compounds); or evaluating alternative uses for the biogas. However, geopolitical conditions must be taken into account, since the Pacific region of Colombia is not fully connected to the natural gas pipeline.  The geographic valley of the Cauca River has six annexed distilleries with installed ethanol capacities between 100,000 and 400,000 L/day, corresponding to vinasse production rates between 6 and 12.5 m 3 /h. Their corresponding vinasse flow rates are as follows: Mill I-6 m 3 /h; Mill II-12.5 m 3 /h; Mill III-10 m 3 /h; Mill IV-12.5 m 3 /h; Mill V-5 m 3 /h; and Mill VI-10 m 3 /h. According to these results, none of the distilleries generate enough vinasse to implement a feasible vinasse-fed AD plant for electrical energy generation. However, based on the distilleries' geographical distribution (Figure 9), an alternative to increasing vinasse processing flow rates is to join vinasse streams between neighboring mills, such as Mills I-IV; this strategy could yield a processing flow rate of 41 m 3 /h. Improving the economic feasibility of a vinasse-fed AD plant while maintaining lower flow rates can also be achieved by the following strategies: increasing the C/N ratio of the substrate; increasing its biomethane potential by incorporating unit operations to valorize alternative waste streams, namely, the carbon dioxide present in the biogas, which reduces its calorific value (and through carbon-capture technologies, it is possible to exploit it or synthesize high-value compounds); or evaluating alternative uses for the biogas. However, geopolitical conditions must be taken into account, since the Pacific region of Colombia is not fully connected to the natural gas pipeline. The geographic valley of the Cauca River has six annexed distilleries with installed ethanol capacities between 100,000 and 400,000 L/day, corresponding to vinasse production rates between 6 and 12.5 m 3 /h. Their corresponding vinasse flow rates are as follows: Mill I-6 m 3 /h; Mill II-12.5 m 3 /h; Mill III-10 m 3 /h; Mill IV-12.5 m 3 /h; Mill V-5 m 3 /h; and Mill VI-10 m 3 /h. According to these results, none of the distilleries generate enough vinasse to implement a feasible vinasse-fed AD plant for electrical energy generation. However, based on the distilleries' geographical distribution ( Figure  9), an alternative to increasing vinasse processing flow rates is to join vinasse streams between neighboring mills, such as Mills I-IV; this strategy could yield a processing flow rate of 41 m 3 /h. Improving the economic feasibility of a vinasse-fed AD plant while maintaining lower flow rates can also be achieved by the following strategies: increasing the C/N ratio of the substrate; increasing its biomethane potential by incorporating unit operations to valorize alternative waste streams, namely, the carbon dioxide present in the biogas, which reduces its calorific value (and through carboncapture technologies, it is possible to exploit it or synthesize high-value compounds); or evaluating alternative uses for the biogas. However, geopolitical conditions must be taken into account, since the Pacific region of Colombia is not fully connected to the natural gas pipeline.  In 2014, Moraes and collaborators [60] used Excel ® to perform an economic assessment of autonomous and annexed plants in Brazil to evaluate the economic feasibility of electrical energy generation from sugarcane vinasse. They reported a NPV of 4,000,000 USD and an IRR of 12% for an annexed distillery with a vinasse-processing flow rate of 80 m 3/ h. By contrast, our results show that an annexed distillery with a vinasse-processing flow rate of 80 m 3/ h generating 3600 m 3/ h of biogas will have an NPV of~10,000,000 USD and a 26.5% IRR. In terms of economic assessment, both studies indicate the profitability of a sugarcane-based AD-plant; however, a low IRR indicates higher investment cost. SuperPro Designer ® projected a higher NPV and IRR, possibly due to the geographical differences between the countries, which directly affect the biochemical characteristics of the feedstock. Additionally, the robustness of the databases and the assumptions used for the analysis imbedded in SuperPro Designer ® , for equipment sizing and costing selection, could explain the resulting differences.
The process simulation of sugarcane-based vinasse-fed AD plants using Aspen Plus ® is still an open field. Lorenzo et al. (2019) [57] developed a process simulation considering an upflow anaerobic sludge blanket (UASB) model using sugarcane-based vinasse as feedstock. They employed stoichiometric reactions from previous reports using the MATLAB ® software, and the reaction kinetics were calculated in FORTRAN. The above work is an example of the ASPEN Plus ® requirements regarding prior established kinetic models and complementary analytical software, which limits its applications by non-experts.
In summary, SuperPro Designer ® is a simple and easy-to-use software program used for large-scale feasibility evaluations of bioprocesses, and its built-in features make it an ideal tool for general use. The software decreases the computational time, because it does not require the integration of additional formulas, and enables the user to assess a larger number of scenarios.

Conclusions
SuperPro Designer ® is a convenient tool for non-experts for evaluating the feasibility of bioprocesses such a vinasse-fed AD-plant. By using its built-in features to perform a TEA, it is possible to identify cost-sensible factors during the technological development and implementation stages. The above was supported by the results, showing that a vinasse-fed AD plant in Colombia is economically feasible when the processing flow rate is >25 m 3 /h, which corresponds with previous studies performed using ASPEN Plus ® and Excel ® [49][50][51][54][55][56][57]. Taking into account that the vinasse flow rates produced by Colombian distilleries are below the mentioned threshold, we proposed joining vinasse-waste streams to overcome the flow rate limitation. However, alternative biogas uses can be assessed-the incorporation of unit operations associated with the production of additional high-value products from non-valorized streams, such as carbon dioxide, or possibly increasing the biomethane potential via C/N balancing. Finally, we identified an inversely proportional relationship between the biomethane potential of sugarcane-based vinasse and its solid content. This effect may be due to the concentration of inhibitory compounds during the evaporation steps, which affects the economic feasibility of an AD plant. However, since there was no difference between the biomethane potential of vinasse at different distilleries, it is technically feasible to join vinasse streams in a single plant.

Conflicts of Interest:
The authors declare no conflict of interest, and they are not in any way connected with SuperPro Designer ® or Intelligen, Inc.