O-Glycosyltransferase Gene BnaC09.OGT Involved in Regulation of Unsaturated Fatty Acid Biosynthesis for Enhancing Osmotic Stress Tolerance in Brassica napus L.

Osmotic stress is a major threaten to the growth and yield stability of Brassica napus. Post-translational modification with O-linked β-N-acetylglucosamine (O-GlcNAc) is ubiquitous in plants, and participates in a variety of signal transduction and metabolic regulation. However, studies on the role of O-GlcNAc transferase (OGT) in osmotic stress tolerance of plants are limited. In previous study, a O-glycosyltransferase, named BnaC09.OGT, was identified from the B. napus variety ‘Zhongshuang 11’ by yeast one hybrid with promoter of BnaA01.GPAT9. It was found that BnaC09.OGT localized in both nucleus and cytoplasm. The spatiotemporal expression pattern of BnaC09.OGT exhibited tissue specificity in developmental seed, especially in 15 days after pollination. In view of osmotic stress inducing, the BnaC09.OGT overexpression and knockout transgenic lines were constructed for biological function study. Phenotypic analysis of BnaC09.OGT overexpression seedlings demonstrated that BnaC09.OGT could enhance osmotic stress tolerance than WT and knockout lines in euphylla stage under 15% PEG6000 treatment after 7 days. In addition, compared with WT and knockout lines, overexpression of BnaC09.OGT had significantly higher activities of antioxidant enzymes (SOD and POD), higher content of soluble saccharide, and while significantly less content of malondialdehyde, proline and anthocyanidin under 15% PEG6000 treatment after 7 days. On the other hand, the unsaturated fatty acid content of BnaC09.OGT overexpression was significantly higher than that of WT and knockout lines, so it is speculated that the BnaC09.OGT could increase unsaturated fatty acid biosynthesis for osmotic stress tolerance by promoting the expression of BnaA01.GPAT9 in glycerolipid biosynthesis. In summary, the above results revealed that the function of BnaC09.OGT provides new insight for the analysis of the pathway of O-glycosylation in regulating osmotic stress tolerance in B. napus.


Introduction
Brassica napus L. is one of the most important oil crops in the world.The entire growth and development period is sensitive to water deficiency [1,2].Osmotic stress inhibits metabolic processes and thereby reduces biomass accumulation and disrupts seed formation, and ultimately resulting in reduced yield [3,4].Osmotic stress has a major limiting impact on its production and yield stability, and accounts for a 30% yield loss at least in B. napus every year [3,5].To ensure crop production and quality under limited conditions, developing resistant varieties is an efficient strategy [6,7].
Glycosyltransferases (GTs), which catalyze glycosyl groups to various receptor molecules, including proteins, carbohydrates, lipids, hormones, and flavonoids [8][9][10], are widely present in organisms.This catalytic process alters the biological ctivity, stability, solubility, and transport characteristics of the receptor molecules [11,12], playing a crucial role Plants 2024, 13,1964 2 of 13 in maintaining intracellular homeostasis.Additionally, the glycosylated products generated have numerous biological functions.Based on the similarity of amino acid sequences of glycosyltransferases, the specificity of catalytic substrates, and the stereochemical structure of the catalytic products, the glycosyltransferases are classified into 135 families according to Carbohydrate-Active Enzymes Database [13].The largest and most extensively studied branch in plants is GT1.There is a subclass known as UDP-glucosyltransferases (UGTs) due to their utilization of UDPG as the glycosyl donor [14].
It is found that glycosylation modifications of certain small molecule metabolites are related to the regulation of plant growth and development and stress resistance [15].In Arabidopsis thaliana, glycosyltransferases UGT79B2 and UGT79B3 were involved in glycosylating anthocyanins, and mutations in these enzymes impacted the accumulation of anthocyanins, which reducing the tolerance to adverse conditions such as salt, drought, and cold stress [16].After overexpression of UGT75D1 in A. thaliana, there is an enhanced tolerance to osmotic stress during seed germination, leading to increased germination rates [17].Research reported that the UDP-glucosyltransferase GSA1 in rice exhibited glycosyltransferase activity towards flavonoid substrates, mediating auxin transport by affecting flavonoid content, ultimately regulating rice grain size [18].
Based on the types of glycosidic bonds, protein glycosylation can be classified into four types of glycosyltransferases: OGT, NGT, CGT, and SGT, with OGT being the predominant type in plants [19].O-glycosylation typically occurs on the oxygen atoms of serine or threonine side chains [20].O-glycosylation interacts with other PTMs (various types of posttranslational modifications) [21,22].O-GlcNAcylation levels are regulated by O-GlcNAc transferase (OGT) and O-GlcNAcase (OGA).The dynamic and reversible modification of glycosylation, serving as a crucial regulatory factor in cellular processes such as signal transduction, transcription, translation [21][22][23], and the involvement of substrate proteins in multiple cellular signaling pathways [22].
It is also found that glycosylation reactions played a crucial role in plant growth and development, secondary metabolism, hormone balance, and responsed to biotic and abiotic stresses [15,24].There is limited research on O-GlcNAcylation in plants, and the role of O-GlcNAc modification in plant responses to osmotic stress remains to be investigated.Hereby, a O-glycosyltransferase was identified in B. napus, which is classified as OGT and belongs to the GT90 family.Furthermore, the transgenic lines with overexpression and knockout of BnaC09.OGT were constructed for biological function study.It was speculated that BnaC09.OGT may enhance osmotic stress tolerance by improving the ROS scavenging capacity and increasing the proportion of unsaturated fatty acids in B. napus, providing new insights into the pathways by which O-GlcNAc modification regulates plant osmotic stress tolerance.

Identification of BnaC09.OGT and Phylogenetic Analysis
The BnaC09.OGT gene was cloned from the cDNA of B. nupus by reverse transcript PCR.The sequence length of BnaC09.OGT was 1743 bp (Supplementary Figure S1).It was deduced that the coding sequence of the BnaC09.OGT gene encodes 580 amino acids, and the protein encoded with a molecular weight of 66.9 kDa by SnapGene software.The conserved domain characteristic of the GT90 glycosyltransferase family was found in protein sequence.Phylogenetic analysis showed that the closest evolutionary relatives of BnaC09.OGT, arranged from nearest to furthest, were: Brassica oleracea, Brassica rapa, Raphanus sativus, Arabidopsis lyrata, Arabidopsis thaliana, Capsella rubella, Camelina sativa (Figure 1).

Subcellular Localization of BnaC09.OGT
To determine the subcellular localization of the BnaC09.OGT protein, a recombination plasmid of pCAMBIA1305.1-BnaC09.OGT-GFP was constructed.Then, the BnaC09.OGT-GFP fusion protein was transiently expressed in Nicotiana.tabacum leaves.The result showed that the green fluorescence signal of fusion protein was located in both the nucleus and cytoplasm by laser confocal microscope (Figure 2).

Subcellular Localization of BnaC09.OGT
To determine the subcellular localization of the BnaC09.OGT protein, a recombination plasmid of pCAMBIA1305.1-BnaC09.OGT-GFP was constructed.Then, the BnaC09.OGT-GFP fusion protein was transiently expressed in Nicotiana.tabacum leaves.The result showed that the green fluorescence signal of fusion protein was located in both the nucleus and cytoplasm by laser confocal microscope (Figure 2).

Subcellular Localization of BnaC09.OGT
To determine the subcellular localization of the BnaC09.OGT protein, a recombination plasmid of pCAMBIA1305.1-BnaC09.OGT-GFP was constructed.Then, the BnaC09.OGT-GFP fusion protein was transiently expressed in Nicotiana.tabacum leaves.The result showed that the green fluorescence signal of fusion protein was located in both the nucleus and cytoplasm by laser confocal microscope (Figure 2).

Tissue-Specific Expression Analysis of BnaC09.OGT
The expression patterns of BnaC09.OGT were analyzed by RT-qPCR in root, stem, leaf, flower, and seed of different developmental stages (15,20,25,30,35, 40 days after pollination).The result indicated that the relative expression of BnaC09.OGT was higher in the seed at 15 days after pollination, reaching 5.4 times than that in the leaf (Figure 3).

Tissue-Specific Expression Analysis of BnaC09.OGT
The expression patterns of BnaC09.OGT were analyzed by RT-qPCR in root, stem, leaf, flower, and seed of different developmental stages (15,20,25,30,35, 40 days after pollination).The result indicated that the relative expression of BnaC09.OGT was higher in the seed at 15 days after pollination, reaching 5.4 times than that in the leaf (Figure 3).

Analysis of the Expression Pattern of the BnaC09.OGT Gene under Osmotic Stress and Exogenous Hormone Treatments
To ascertain the response to osmotic stress and hormone treatments of the BnaC09.OGT gene, the three-leaf stage of 'Zhongshuang 11' was subjected to osmotic stress and external application of ABA, IAA, and MeJA, using untreated wild types as controls.Measured the realative expression levels of the BnaC09.OGT in leaves under different treatment by RT-qPCR.It was found that the expression of BnaC09.OGT peaked at 12 h under osmotic stress, reaching 5.5 times than that of the control (Figure 4A).With ABA IAA, and MeJA treatments, the expression of BnaC09.OGT also peaked at 12 h after treatment, reaching 3.6, 3.4, and 3.6 times than that of the control, respectively (Figure 4B-D).

Analysis of the Expression Pattern of the BnaC09.OGT Gene under Osmotic Stress and Exogenous Hormone Treatments
To ascertain the response to osmotic stress and hormone treatments of the BnaC09.OGT gene, the three-leaf stage of 'Zhongshuang 11' was subjected to osmotic stress and external application of ABA, IAA, and MeJA, using untreated wild types as controls.Measured the realative expression levels of the BnaC09.OGT in leaves under different treatment by RT-qPCR.It was found that the expression of BnaC09.OGT peaked at 12 h under osmotic stress, reaching 5.5 times than that of the control (Figure 4A).With ABA IAA, and MeJA treatments, the expression of BnaC09.OGT also peaked at 12 h after treatment, reaching 3.6, 3.4, and 3.6 times than that of the control, respectively (Figure 4B-D).

Construction and Identification of Transformants
Two recombinant plasmid, pCAMBIA1305.1-35S-BnaC09.OGT-NOS and PKSE401-TRNA were constructed (Supplementary Figure S2), and used to Agrobacterium tumefaciens-mediated for hypocotyl genetic transformation (Supplementary Figure S3).17 positive transgenic lines of BnaC09.OGT overexpression was identified by specific PCR (Supplementary Figure S4).Then, T1 generation obtained by self-pollination in these lines, and used to screen single-insert transgenic lines with hygromycin (30 μg/mL).Three high expression and single insertion lines, BnaC09.OGT-OE1, OE10 and OE14 (Supplementary Figure S5), were selected for further experiments.On the other hand, two knockout lines of BnaC09.OGT were identified by high-throughput sequencing and created self-pollination T1 generation for subsequent experiments (Supplementary Figure S6).

Characterization of BnaC09.OGT Transformants with Osmotic Stress
For osmotic stress, the seedling at four euphylla stage of BnaC09.OGT-OE1/OE10/OE14 and BnaC09.OGT-KO1/KO2 T1 generation independent lines were transferred into 1/5 strength Hoagland's solution with 15% (w/v) PEG6000 (−0.3 Mpa).Plant phenotypic and related physiological parameters were measured after 7 days osmotic stress treatment.During the early vegetative growth stage, the biomass of shoot was positively correlated with osmotic stress resistance.After treatment, the leaves of the BnaC09.OGT-OE independent lines were larger than those of the WT and BnaC09.OGT-KO independent lines, and the root length also showed the same exhibition in these

Construction and Identification of Transformants
Two recombinant plasmid, pCAMBIA1305.1-35S-BnaC09.OGT-NOS and PKSE401-TRNA were constructed (Supplementary Figure S2), and used to Agrobacterium tumefaciensmediated for hypocotyl genetic transformation (Supplementary Figure S3).17 positive transgenic lines of BnaC09.OGT overexpression was identified by specific PCR (Supplementary Figure S4).Then, T 1 generation obtained by self-pollination in these lines, and used to screen single-insert transgenic lines with hygromycin (30 µg/mL).Three high expression and single insertion lines, BnaC09.OGT-OE1, OE10 and OE14 (Supplementary Figure S5), were selected for further experiments.On the other hand, two knockout lines of BnaC09.OGT were identified by high-throughput sequencing and created self-pollination T 1 generation for subsequent experiments (Supplementary Figure S6).

Characterization of BnaC09.OGT Transformants with Osmotic Stress
For osmotic stress, the seedling at four euphylla stage of BnaC09.OGT-OE1/OE10/OE14 and BnaC09.OGT-KO1/KO2 T 1 generation independent lines were transferred into 1/5 strength Hoagland's solution with 15% (w/v) PEG6000 (−0.3 Mpa).Plant phenotypic and related physiological parameters were measured after 7 days osmotic stress treatment.During the early vegetative growth stage, the biomass of shoot was positively correlated with osmotic stress resistance.After treatment, the leaves of the BnaC09.OGT-OE independent lines were larger than those of the WT and BnaC09.OGT-KO independent lines, and the root length also showed the same exhibition in these transformants and WT (Figure 5).The number of euphylla in overexpression seedlings was significantly more than that of BnaC09.OGT-KO and WT lines after 14 days osmotic stress treatment (Table 1).

Physiological Responses to Osmotic Stress
The enzymatic activity of SOD and POD were calculated to further investigate the effect of osmotic stress in transformants.The results showed that BnaC09.OGT-OE independent lines had a significantly higher SOD and POD enzyme activity than BnaC09.OGT-KO independent lines and WT (Figure 6A,B).Meanwhile, more MDA accumulation was found in the BnaC09.OGT-OE independent lines (Figure 6C).In addition, proline levels increased in all lines after 7 days drought treatment, but the BnaC09.OGT-OE lines had lower proline content than WT, while the BnaC09.OGT-KO lines had higher content (Figure 6D).Soluble saccharide content was significantly increased in the BnaC09.OGT-OE lines compared to WT, and significantly decreased in the BnaC09.OGT-KO lines (Figure 6E).The chlorophyll content in all lines decreased after osmotic stress, with the greatest reduction in the BnaC09.OGT-KO lines and the least reduction in the BnaC09.OGT-OE

Physiological Responses to Osmotic Stress
The enzymatic activity of SOD and POD were calculated to further investigate the effect of osmotic stress in transformants.The results showed that BnaC09.OGT-OE independent lines had a significantly higher SOD and POD enzyme activity than BnaC09.OGT-KO independent lines and WT (Figure 6A,B).Meanwhile, more MDA accumulation was found in the BnaC09.OGT-OE independent lines (Figure 6C).In addition, proline levels increased in all lines after 7 days drought treatment, but the BnaC09.OGT-OE lines had lower proline content than WT, while the BnaC09.OGT-KO lines had higher content (Figure 6D).Soluble saccharide content was significantly increased in the BnaC09.OGT-OE lines compared to WT, and significantly decreased in the BnaC09.OGT-KO lines (Figure 6E).The chlorophyll content in all lines decreased after osmotic stress, with the greatest reduction in the BnaC09.OGT-KO lines and the least reduction in the BnaC09.OGT-OE lines (Figure 6F).The anthocyanin content increased in the WT, BnaC09.OGT-OE, and BnaC09.OGT-KO lines, while the rate of increase in the BnaC09.OGT-OE lines was significantly lower than WT and BnaC09.OGT-KO lines (Figure 6G).In addition, the intensity of NBT (Nitro Blue Tetrazolium) staining usually reflects the accumulation of superoxide anions in cells.It was found that the BnaC09.OGT-OE lines showed the lightest staining under 7 days osmotic stress, which indicated the least accumulation of reactive oxygen species, while the BnaC09.OGT-KO lines showed the darkest staining, indicating more accumulation of reactive oxygen species (Figure 7).

Fatty Acid Component of Transformants under Osmotic Stress
There was no significant difference in the ratio of total unsaturated fatty acids to total saturated fatty acids among WT, BnaC09.OGT-OE and BnaC09.OGT-KO under osmotic stress (Table 2).However, the results exhibited significant difference in the ratio after 7 days osmotic stress, the content of total unsaturated fatty acids decreased in BnaC09.OGT-KO, while increased in the BnaC09.OGT-OE lines (Table 2).

Fatty Acid Component of Transformants under Osmotic Stress
There was no significant difference in the ratio of total unsaturated fatty acids to total saturated fatty acids among WT, BnaC09.OGT-OE and BnaC09.OGT-KO under osmotic stress (Table 2).However, the results exhibited significant difference in the ratio after 7 days osmotic stress, the content of total unsaturated fatty acids decreased in BnaC09.OGT-KO, while increased in the BnaC09.OGT-OE lines (Table 2).

Discussion
The O-glycosyltransferase SECRET AGENT (SEC) showed a high alignment of catalytic active sites in plants, which indicated it was a conserved evolution of the OGT gene [25].BnaC09.OGT was localized in the nucleus and cytoplasm, with potential involvement in the modification of nuclear-localized proteins and soluble proteins [25,26].The AtUGT71C5 could glycosylate ABA to deactivate it.The mutant of AtUGT71C5 geneshowed that glycosylation levels of ABA were reduced, resulting in increased levels of active ABA, which enhances the tolerance of osmotic stress [27].In addition, JA was involved in regulating stomatal closure under drought conditions [28].Studies have shown that auxins are related to plant drought tolerance.Overexpression of DgIAA21 reduced the drought tolerance of transgenic Arabidopsis [29].The expression of the BnaC09.OGT gene was significantly induced by osmotic stress, and its expression was also significantly increased under exogenous treatments with hormones, such as ABA, MeJA, and IAA.These results suggested that the involvement of osmotic stress tolerance by BnaC09.OGT may be related to the regulation of plant hormone levels.It is speculated that these hormones could be glycosylated to involving the osmotic signal pathway.
Plants 2024, 13, 1964 9 of 13 it was found that the enhanced osmotic stress tolerance of the BnaC09.OGT overexpression lines may be due to an increased capacity for ROS scavenging and a higher proportion of unsaturated fatty acids in the plants.The antioxidant defense system is one of the abiotic stress response mechanisms.There is a positive correlation between antioxidant enzyme activity and abiotic stress resistance in plant.Under osmotic stress, reactive oxygen species accumulated in the cell membranes could oxidize the unsaturated fatty acids on the glycerol backbone of the membranes, leading to increased membrane permeability, with the level of stress increased, lipid peroxidation intensifies, damaging membrane proteins and lipids, ultimately impairing the normal physiological function of the cell membranes [30,31].After osmotic stress, enzymatic activity of SOD, POD significantly increased in the BnaC09.OGT-OE lines.Nitro Blue Tetrazolium (NBT) staining results also showed that the BnaC09.OGT-OE lines exhibited reduced reactive oxygen species levels compared to the control under osmotic stress.The BnaC09.OGT overexpression lines may enhance plant osmotic stress tolerance by increasing antioxidant enzyme activity and reducing ROS levels.The results of decreased MDA, proline, anthocyanin content, and less reduction in chlorophyll content indicated that the oxidative stress level of BnaC09.OGT overexpression lines is lower after osmotic stress, thereby enhancing the resistance of plants.After osmotic stress treatment, the content of unsaturated fatty acids in the BnaC09.OGT-OE lines was significantly higher than that of the wild type and BnaC09.OGT-KO lines.In addition, phosphatidic acid and phosphatidyl choline, which can affect fatty acid composition, were also presumed to be the substrates of glycosyltransferase for involving the phosphatidic acid signaling pathway and glycolipid synthesis through glycosylation.Thereby, phosphatidic acid and phosphatidyl choline may be an important role in maintaining the integrity of cell membrane structures in plants and ultimately affecting plant osmotic stress tolerance.

Plant Materials
The B. napus variety 'Zhongshuang 11' was used as the plantlet material in the experiments.The hypocotyl was used to genetic transformation as the explant.

BnaC09.OGT Clone and Phylogenetic Analysis
The coding sequences of BnaC09.OGT were amplified using the High-Fidelity Enzyme PrimeSTAR Kit (Takara, Japan) with the specific primers listed in Supplementary Table S1.BnaC09.OGT sequences of different species were obtained by BLAST (https://blast.ncbi.nlm.nih.gov/Blast.cgi, 5 November 2023), multiple sequence alignment was performed using DNAMAN.Sequence alignment was performed using Clustal W, followed by the construction of a phylogenetic tree using the neighbor-joining (NJ) method with 1000 bootstraps.

Subcellular Localization
Homologous recombination primers were designed using the vector pCAMBIA1305.1-35S-GFP(modified from pCAMBIA1305.1 with a GFP reporter gene by our laboratory) and the BnaC09.OGT coding sequence (Supplementary Table S1).The leaf epidermal cells of N. tabacum were transiently infected via A. tumefaciens-mediated transformation, with GFP fluorescence signals observed after 36 h of cultivation by confocal microscope [32].

Analysis of the Expression Pattern of the BnaC09.OGT Gene under Osmotic Stress and Various Exogenous Hormone Treatments
To determine the expression pattern of the BnaC09.OGT gene under osmotic stress and various plant hormone treatments, the seeds of 'Zhongshuang 11' was grown in 1/5 Hoagland solution until the three-leaf stage.The plants were cultured at a temperature of 24 • C with a photoperiod of 16 h/8 h (light/dark), the light intensity is 300-500 µmol m −2 s −1 , and the relative humidity is 60%.The seedlings were subjected to drought treatment using 15% PEG6000 (−0.3 Mpa) replacing the hydroponic solution.Exogenous hormones were applied by spraying solutions of 100 µmol/L IAA, 50 µmol/L ABA, and 100 µmol/L MeJA on the surface of the seedling leaves.Samples were taken from the leaves at 0, 12 and 24 h after treatment, and the relative expression levels of the BnaC09.OGT were measured using RT-qPCR, with 12 seedlings sampled per treatment and repeated three times.

Construction and Identification of Transformants
Restriction enzymes NcoI and MluI were used to identified the pCAMBIA1305.1-35S-BnaC09.OGT-NOS plasmid, which was constructed through homologous recombination with the sequences listed in Supplementary Table S1.The recombinant plasmid was then transferred into GV3101.Transgenic seedlings were obtained using B. napus variety 'Zhongshuang 11' as the recipient through hypocotyl transformation [35].The T 0 generation transgenic seedlings were screened by PCR, and those positive seedlings were transplanted to the field until T 1 generation seeds were harvested.The harvested T 1 seeds were sterilized, sown on 1/2MS solid medium containing 30 µg/mL Hyg, and cultured for 7 days.A chisquare test was used to verify the ratio of resistant to non-resistant seedlings.The single insertion site of the transformant was calculated to 3:1 ratio.Two CRISPR target sites were designed using the website http://crispr.hzau.edu.cn/cgi-bin/CRISPR2/CRISPR(accessed on 30 June 2024).Primers for amplifying the target sites were designed and synthesized.Using the PGTR plasmid as a template, amplification was performed, and the PCR products were incorporated into the final CRISPR expression vector PKSE401-TRNA through homologous recombination.The recombinant plasmid was then transferred into GV3101, with B. napus variety 'Zhongshuang 11' served as the recipient, and gene-edited seedlings were obtained through hypocotyl transformation.

Characterization and Physiological Responses to Osmotic Stress of BnaC09.OGT Transformants
Select T 1 generation of transgenic lines seeds for the experiment, with the B. napus variety 'Zhongshuang 11' served as the WT (wild type).The transgenic lines include BnaC09.OGT-OE1/10/14: the independent lines of pCAMBIA1305.1-35S-BnaC09.OGT-NOS transgenic lines in 'Zhongshuang 11' genetic background; and BnaC09.OGT-KO1/KO2: the independent lines of transformant with BnaC09.OGT knockout in 'Zhongshuang 11' genetic background.After vernalization, seeds were sown in germination boxes and grown until the cotyledons unfolded and the hypocotyl elongated, then transferred to 1/5 Hoagland solution for cultivation.When seedlings reached the stage of having four fully unfolded true leaves, similarly grown seedlings were transferred into 15% (w/v) PEG6000 solution prepared with 1/5 Hoagland solution to simulate a −0.3 MPa osmotic stress condition.After 7 days treatment, plant morphology was observed and related physiological parameters were measured.

Figure 2 .
Figure 2. Subcellular localization of BnaC09.OGT.GFP: GFP channel; mCherry: red fluorescence in the nucleus; Bright field: light microscopy image; Merged: merged image of the GFP and Bright channels.

Figure 2 .
Figure 2. Subcellular localization of BnaC09.OGT.GFP: GFP channel; mCherry: red fluorescence in the nucleus; Bright field: light microscopy image; Merged: merged image of the GFP and Bright channels.

Figure 2 .
Figure 2. Subcellular localization of BnaC09.OGT.GFP: GFP channel; mCherry: red fluorescence in the nucleus; Bright field: light microscopy image; Merged: merged image of the GFP and Bright channels.

Table 1 .
Statistics on the number of euphylla in wild type, independent BnaC09.OGT-OE and BnaC09.OGT-KO lines under osmotic stress.

Table 2 .
The proportion of unsaturated fatty acids in leaves of wild type, independent BnaC09.OGT-OE and BnaC09.OGT-KO lines under osmotic stress with 15% PEG6000.