Biological Activities and Chemical Profiles of Kalanchoe fedtschenkoi Extracts

In this study, the leaves of Kalanchoe fedtschenkoi were consecutively macerated with hexane, chloroform, and methanol. These extracts were used to assess the bioactivities of the plant. The antimicrobial activity was tested against a panel of Gram-positive and -negative pathogenic bacterial and fungal strains using the microdilution method. The cytotoxicity of K. fedtschenkoi extracts was investigated using human-derived macrophage THP-1 cells through the MTT assay. Finally, the anti-inflammatory activity of extracts was studied using the same cell line by measuring the secretion of IL-10 and IL-6. The phytoconstituents of hexane and chloroform extracts were evaluated using gas chromatography–mass spectrometry (GC/MS). In addition, high-performance liquid chromatography (HPLC) was used to study the phytochemical content of methanol extract. The total flavonoid content (TFC) of methanol extract is also reported. The chemical composition of K. fedtschenkoi extracts was evaluated using Fourier-transform infrared spectroscopy (FTIR). Results revealed that the chloroform extract inhibited the growth of Pseudomonas aeruginosa at 150 μg/mL. At the same concentration, methanol extract inhibited the growth of methicillin-resistant Staphylococcus aureus (MRSA). Regarding their cytotoxicity, the three extracts were highly cytotoxic against the tested cell line at IC50 < 3 μg/mL. In addition, the chloroform extract significantly stimulated the secretion of IL-10 at 50 μg/mL (p < 0.01). GC/MS analyses revealed that hexane and chloroform extracts contain fatty acids, sterols, vitamin E, and triterpenes. The HPLC analysis demonstrated that methanol extract was constituted by quercetin and kaempferol derivatives. This is the first report in which the bioactivities and chemical profiles of K. fedtschenkoi are assessed for non-polar and polar extracts.


Introduction
Traditional medicine comprises knowledge, practices, and beliefs that, when integrated, are useful to treat or avert multiple diseases. Given its accessibility and affordability, traditional medicine is utilized as a first line of response against medical emergencies in African countries [1]. In traditional medicine, syrups, decoctions, infusions, and extracts from herbs or medicinal plants are used as antimicrobial, anticancer, anti-inflammatory, antidepressant, or antiaggregant agents [2].
Medicinal plants and their parts are widely used to prepare therapeutic extracts [3]. In traditional medicine, medicinal plants are predominantly utilized to treat diseases, as they are a cost-effective alternative that exerts lower side effects than current treatment

HPLC Analysis
HPLC is a proper technique in which analytes dissolved in a mobile phase are pumped through a stationary phase. Depending upon the chemical features of the sample, solvent, and stationary phase, analytes exhibit different retention times (Rt). Here, we used HPLC to study the chemical composition of methanol extract from K. fedtschenkoi. As presented in the Supplementary Materials ( Figure S3), the chromatogram of methanol extract exhibits characteristic peaks corresponding to flavonoids identified in other reports in which K. fedtschenkoi has also been studied [25,26]. In accordance with their results, we show that methanol extract also contains quercetin di-O-hexoside (Rt: 14 On the other hand, the same chromatogram also presents a small peak at 38.54 min and a sharp peak at 47.23 min, which, comparably to HPLC analyses of extracts from K. brasiliensis, might suggest the presence of patuletin-O-deoxy-hexoside-O-acetyl-deoxy-hexoside [34]. To estimate the amount of flavonoids in the methanol extract, we performed the TFC assay.

TFC of Methanol Extract from K. fedtschenkoi
The TFC assay is a widely performed colorimetric method required to assess the presence of flavonoids in plant extracts. This technique is based on aluminum chloride (AlCl 3 )'s capacity to form complexes with hydroxyl and carbonyl groups from various flavonoids.
Flavonoids constitute a broad category of secondary metabolites. Structurally, flavonoids are formed by two benzene rings (A and B) joined by a three-carbon-based pyran ring (C). According to their substitution pattern and the number of functional groups, flavonoids are categorized into anthocyanidins, flavonols, flavones, and isoflavones. It is known that Kalanchoe species can contain multiple flavonoids such as quercetin (Qu), kaempferol, myricetin, luteolin, eupafolin, or their derivatives [35]. These compounds have been reported among several species, for example, K. pinnata, K. gracilis, K. blossfeldiana, K. tomentosa, and K. pathulate [19,20,36].
A calibration curve was constructed considering various concentrations of Qu to estimate the TFC of methanol extract from K. fedtschenkoi. To perform the TFC assay, bioactive nature products such as catechin and rutin are commonly used. However, Qu is also preferred, as it is a flavonol that reacts with AlCl 3 due to its keto group at C4 and hydroxyl groups at C3 or C5. Therefore, using the regression equation (y = 0.0007x + 0.3518, R 2 = 0.9995) presented in the Supplementary Materials ( Figure S4), the TFC of this extract was estimated and represented in milligrams of quercetin equivalents per gram of the plant extract (mg Qu/g). In this regard, y was considered as the absorbance of the test sample, whereas x was appraised as the concentration from the calibration curve. Following our calculations, the TFC of methanol extract is 384.54 ± 2.25 mg Qu/g. This result can be comparable to the TFC of methanol extracts prepared from other Kalanchoe species, such as K. pinnata (106 mg Qu/g) and K. integra (178 mg Qu/g) [37].

Antimicrobial Activity
In this study, the chloroform and methanol extract from K. fedtschenkoi exhibited weak antimicrobial activity against the panel tested (see Table 2). For instance, treatment with 150 µg/mL of chloroform extract only inhibited the growth of P. aeruginosa. At the same concentration, methanol extract inhibited the growth of MRSA. No inhibition of yeast strains was recorded.
Medicinal plants can exhibit antimicrobial properties against pathogenic bacteria, fungi, protozoa, and viruses through bioactive secondary metabolites such as alkaloids, flavonoids, terpenes, and polysaccharides. Generally, extracts from medicinal plants or herbs can inhibit the growth of pathogenic bacteria and fungi by damaging cell membranes or walls, interfering with protein synthesis, and increasing intracellular osmotic pressure [38]. These mechanisms are due to the phytochemical content of plant extracts; for example, given the existence of hydroxyl groups and delocalized electrons among polyphenols' architecture, they can increase the permeability of the bacterial membrane, alter its potential, and cause structural changes [39]. In contrast, the acyl chains, numerous hydroxyl groups, and glycosylated moieties of flavonoids enable their capacity to reduce nucleic acid synthesis, disrupt energy metabolisms, and suppress cytoplasmic bacterial membrane functionality [40]. Table 2. Antimicrobial activity of K. fedtschenkoi extracts expressed as the minimal inhibitory concentration (µg/mL).

Extract
Bacteria Fungi Among Kalanchoe species, many antibacterial and antifungal compounds have been identified over the last decades. For example, K. pinnata and K. daigremontiana are wellknown medicinal plants that contain flavonols (e.g., quercetin and kaempferol), flavones (e.g., luteolin), and bufadienolides [41] that can reduce the formation of biofilms, inhibit the growth of pathogenic bacteria strains, decrease protein synthesis, or inhibit the expression of genes related to antimicrobial resistance [20,[42][43][44]. According to published reports [45], these mechanisms of action might be related to the glycosyl derivatives of kaempferol identified on the methanol extract of K. fedtschenkoi. In the same regard, extracts from other species, such as K. blossfeldiana, contain palmitic acid, gallic acid, methyl gallate, and carbohydrates that can be correlated to their antimicrobial properties [46].
Among multidrug-resistant bacteria, P. aeruginosa is a challenging pathogen in human health care. It can cause acute or chronic infections in patients diagnosed with cystic fibrosis, cancer, and coronavirus disease-19 (COVID-19) [47]. Only one study has reported the inhibition of P. aeruginosa, using ethanol extracts from K. fedtschenkoi at concentrations ranging from IC 50 128 to 256 µg/mL; similar concentrations were reported against A. baumannii and S. aureus [25]. These findings can be attributed to differences in harvesting places, climate, soil characteristics, extract polarity, implemented methodology, and strain culture conditions. MRSA is a global human health threat characterized by its prevalence in the community, ease of spread, and capacity to cause endocarditis, bacteremia, osteomyelitis, pneumonia, and purulent infections [48]. These results can be compared with the activity of hydroethanolic extracts from K. brasiliensis that have inhibited the growth of MRSA strains at MIC > 5000 µg/mL. The antibacterials eupafolin and patuletin or their glycosylated derivatives can explain this activity [49].

Cytotoxicity Activity
The evaluation of the toxicity of plant extracts or bioactive nature products is necessary to determine their possible application in the development of pharmaceutical formulations or use against other diseases, such as cancer [50]. In this study, we assessed the cytotoxicity using the MTT ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay [51]. Results demonstrated that the three extracts exhibited a significant cytotoxic effect (p < 0.0001) at the tested concentrations ( Figure 1). In this regard, it should be noted that even though treatment with hexane and chloroform extracts was cytotoxic to THP-1 cells, treatment with the methanol extract exhibited the highest cytotoxicity towards the tested cell line (p < 0.0001). formulations or use against other diseases, such as cancer [50]. In this study, we assessed the cytotoxicity using the MTT ((3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay [51]. Results demonstrated that the three extracts exhibited a significant cytotoxic effect (p < 0.0001) at the tested concentrations ( Figure 1). In this regard, it should be noted that even though treatment with hexane and chloroform extracts was cytotoxic to THP-1 cells, treatment with the methanol extract exhibited the highest cytotoxicity towards the tested cell line (p < 0.0001). It is known that medicinal plants have represented an exceptional source of cytotoxic molecules with potential use against various cancers such as lung, breast, and prostate cancer. This is of broad importance in developing countries such as Mexico, since approximately 30% of Mexican patients utilize preparations from medicinal plants as a preventive, complementary, and cost-effective approach during cancer therapy [52]. Furthermore, the structural diversity of isolated molecules from medicinal plants is exploited in developed countries to develop drugs against infectious and neoplastic diseases [53,54].
According to the National Cancer Institute (NCI) of the U.S., the cytotoxicity of molecules can be considered high (IC50 ≤ 20 µg/mL), moderate (IC50 21-200 µg/mL), or weak (IC50 201-500 µg/mL) [55,56]. We found that the hexane, chloroform, and methanol extracts were highly cytotoxic against the tested cell line, as they presented IC50 values of It is known that medicinal plants have represented an exceptional source of cytotoxic molecules with potential use against various cancers such as lung, breast, and prostate cancer. This is of broad importance in developing countries such as Mexico, since approximately 30% of Mexican patients utilize preparations from medicinal plants as a preventive, complementary, and cost-effective approach during cancer therapy [52]. Furthermore, the structural diversity of isolated molecules from medicinal plants is exploited in developed countries to develop drugs against infectious and neoplastic diseases [53,54].
According to the National Cancer Institute (NCI) of the U.S., the cytotoxicity of molecules can be considered high (IC 50 ≤ 20 µg/mL), moderate (IC 50   µg/mL), or weak (IC 50 201-500 µg/mL) [55,56]. We found that the hexane, chloroform, and methanol extracts were highly cytotoxic against the tested cell line, as they presented IC 50 values of 2.090, 1.918, and 1.722 µg/mL, respectively. This result can be attributed to the extracts' polarity and their phytoconstituents.
For instance, the cytotoxicity of the hexane extract might be due to the presence of friedelin, which has been reported to inhibit the proliferation of breast cancer cells [57]. In the same regard, the cytotoxicity of the chloroform extract can be attributed to the presence of stigmasterol and tocopherol. Currently, the former is recognized as a promising cytotoxic agent for cancer therapy [58], whereas the latter can exert distinct cytotoxic effects on cell lines such as bovine endothelial cells, mouse macrophages, and human hepatocytes [59]. On the other hand, the cytotoxicity of the methanol extract might be due to synergism between the derivatives of quercetin and kaempferol, which have been reported to induce the death of different cell lines such as MDA-MB-231 and MCF-7 [60].
Non-polar solvents such as hexane and chloroform are frequently used to extract bioactive compounds such as terpenoids, fats, and oils. In contrast, polar solvents, such as methanol, are preferably used, as they are accessible, nontoxic at low concentrations, and can extract high polar compounds with strong therapeutic properties (e.g., anthocyanins, polyphenols, and flavonoids) such as antimicrobial and anticancer qualities [4,61]. Interestingly, the cytotoxicity of Kalanchoe species has been widely reported for polar extracts in the scientific literature. For instance, treatment with less than <40 µg/mL of K. crenata methanol extract was cytotoxic towards breast adenocarcinoma (MCF-7), hepatocarcinoma (HepG2), colorectal adenocarcinoma (DLD-1), and human non-small-cell lung cancer (A549) cell lines [62].
In another study, comparable effects have been documented for ethanol extracts prepared from the leaves of K. millotii and K. nyikae, which were cytotoxic against human acute lymphoblastic leukemia T (J45) and human T (H9) cell lines at IC 50 values of 503.5 and 560.5, and 846.1 and 507.6 µg/mL, respectively [63]. Against other types of cancer, such as the human ovarian cancer SKOV-3 cells, a water extract from the leaves of K. daigremontiana arrested the cell cycle and induced mitochondrial membrane depolarization. It was cytotoxic against the cells at 5 to 200 µg/mL [32]. Comparably to these reports, our work demonstrated that the K. fedtschenkoi extracts' cytotoxicity increases according to the polarity of the solvents. For instance, treatment with the methanol extract was highly cytotoxic against THP-1 cells at 1.722 µg/mL, whereas treatment with hexane extract decreased cell viability at 2.090 µg/mL. In another study, the cytotoxicity of the ethanol extract from K. fedtschenkoi was evaluated towards human keratinocytes cells (HaCAts); this study revealed that ethanol extract decreased cell viability at LD 50 > 250 µg/mL [25]. However, other differences between our results and other reports can be attributed to the studied species, tested concentrations, extracts' polarity, and evaluated cell lines.

Anti-Inflammatory Activity
Inflammation occurs when hazardous stimuli, such as microorganisms, toxic compounds, or damaged cells, activate immune cells [64]. Multidrug-resistant bacteria can promote inflammation processes through different mechanisms. Inflammatory responses caused by pathogenic bacteria arise from the interaction between pathogen-associated molecular patterns (PAMPs) and pattern-recognition receptors (PRRs), which are structures expressed among immune and non-immune cells [64]. PRRs recognize distinct ligands from bacterial architecture, such as flagellin, peptidoglycans, genetic material, and lipoteichoic acid [65]. The interaction between PAMPs and PRRs results in complex intracellular signaling cascades devoted to recruiting inflammatory molecules necessary to retain the progression of infection and inflammation and to initiate tissue repair [66,67].
In this study, we show that treatment with the K. fedtschenkoi chloroform extract significantly stimulated the secretion of the anti-inflammatory cytokine IL-10 at 50 µg/mL (p < 0.01). Still, it did not reduce the secretion of the pro-inflammatory cytokine IL-6 at the tested concentration ( Figure 2). The anti-inflammatory activity of the chloroform extract can be attributed to the presence of stigmasterol. This widely recognized phytosterol can suppress the production of IL-6 and other pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α) [68,69]. On the other hand, treatment with hexane and methanol extract enhanced the secretion of IL-6, which can be attributed to the cytotoxicity of these extracts. Several models have been proposed to evaluate the anti-inflammatory properties of Kalanchoe species. For example, it has been reported that treatment with K. pinnata hydroethanolic extract reduced pro-inflammatory responses among rodent models by downregulating the expression of mediators involved in inflammatory processes such as Toll-like receptors and nuclear factor kappa B (NFκB) [70]. Comparably, topical formulations prepared from K. pinnata and K. brasiliensis aqueous extracts decreased the production of TNF-α and IL-1β while enhancing the levels of IL-10 among edematogenic Swiss mice models [71].

FTIR Analysis
FTIR spectroscopy is based on the absorption of infrared light by organic and inorganic analytes. Each sample exhibits a distinctive spectrum fingerprint in this technique that can be recognized and differentiated from other molecules [72]. For plant extracts, FTIR can be used to study their chemical composition in solid and liquid samples. Figure 3 depicts K. fedtschenkoi extracts exhibiting similar bands within 2916 and 2849 cm −1 , corresponding to the asymmetrical and symmetrical stretching of the C-H bonds from hydrocarbon chains. Comparably, hexane and methanol extracts present a broad band at 3298 cm −1 , related to O-H bond stretching, usually associated with phenolic compounds. This finding might suggest the presence of compounds reported among K. fedtschenkoi extracts, such as quercetin. Among bioactive nature products, quercetin is an abundant flavonoid in plants, fruits, and vegetables, exhibiting different peaks in the infrared region. It has been reported that flavonoids such as quercetin exhibit distinctive bands from 1610 to 1510 cm −1 , attributed to C=C bonds from its aromatic ring. In addition, it is documented that quercetin presents a series of bands from 1260 to 1160 cm −1 , which is related to the C-O bond stretch aryl ether ring of its structure [73]. Interestingly, FTIR spectra of K. fedtschenkoi extracts present related peaks from 1600 to 1500 cm −1 , which can respond to bending vibrations of C=O bonds, possibly from esters, ketones, and carboxylic acids [74]. These findings are in accord with FTIR analysis of the chemical composition of extracts from other plants, including K. fedtschenkoi [75,76]. acids [74]. These findings are in accord with FTIR analysis of the chemical composition of extracts from other plants, including K. fedtschenkoi [75,76].

Plant Material and Extract Preparation
Aerial parts of K. fedtschenkoi were collected in Texcoco, Mexico (19.491100418879938, −98.88525021669616). Specimens were identified by the biologist Lilián López-Chávez at the herbarium of Universidad Autónoma de Chapingo (Carr. Federal México-Texcoco, 56,230, Texcoco, Estado de México) and deposited with the voucher number 36205. The collected fresh plants were dried in a ventilated and dark place at room temperature for one week. For extract preparation, 500 g of dried leaves were finely powdered utilizing a mechanical blender before being initially macerated with hexane, then with chloroform, and finally, with methanol for three days (1.5 L each). After that, the mixture was filtrated,  collected fresh plants were dried in a ventilated and dark place at room temperature for one week. For extract preparation, 500 g of dried leaves were finely powdered utilizing a mechanical blender before being initially macerated with hexane, then with chloroform, and finally, with methanol for three days (1.5 L each). After that, the mixture was filtrated, and the solvent was evaporated to dryness under reduced pressure using a Heidolph Laborota 4000 efficient rotary evaporator (Schwabach, Germany). The extracts were preserved under refrigeration until further use.

GC/MS Analysis
The phytoconstituents of K. fedtschenkoi extracts were identified using a Varian CP-3800 gas chromatograph coupled to a Varian 1200 quadrupole mass spectrometer [77]. Samples were injected into a Factor Four (VF-5 ms, 30 m × 0.25 mm, 0.25 µm thickness) capillary column. Helium was used as the carrier gas at a 1 mL/min flow rate. The separation was carried out by injecting 1 µL of the sample (1%) into the column at the following gradient temperature: 60 • C for 2 min, 120 • C for 16 min,

HPLC Analysis
The phytochemical content of methanol extract was studied considering reported protocols with minor modifications [26]. Briefly, HPLC analysis was performed on an Agilent Technologies 1200 series equipped with a diode array detector (DAD) utilizing reagents of HPLC grade. An RP-18 Zorbax 150 mm × 4.6 mm, 3.5 µm column was employed as a stationary phase. On the other hand, water acidified with 0.1% formic acid (A) and acetonitrile (B) were used as the mobile phase. To prepare the sample, 2 mg of extract was diluted in water/acetonitrile (5:1). To carry out analysis, 10 µL of sample was run at the following gradient: 0-20 min

TFC Analysis
The TFC of K. fedtschenkoi methanol extract was determined as published [78]. Shortly, 100 µL of methanol extract (1 mg/mL) mixed with 100 µL of 2% aluminum chloride (AlCl 3 ) was incubated for 10 min. Then, using a Cary 60 UV-Vis spectrophotometer (Agilent Technologies, Santa Clara, CA, USA), absorbance was measured at 420 nm in 1 cm quartz cuvettes. A standard curve was obtained using distinct concentrations (100 to 500 µg/mL) of a standard solution of quercetin (Qu). The TFC of methanol extract was estimated as a percentage of total quercetin equivalents per gram of extract (mg Qu/g). The experiment was performed in triplicate.

Strains and Culture Media
The antimicrobial activity of K. fedtschenkoi extracts was studied against a panel of pathogenic Gram-positive and -negative bacteria and yeast strains. Gram-positive bacteria included Listeria monocytogenes (ATCC BAA-679), methicillin-resistant Staphylococcus aureus (MRSA) (ATCC 700698), and Staphylococcus aureus (ATCC 25923). On the other hand, Gramnegative bacteria included Acinetobacter baumannii (ATCC BAA-747), Escherichia coli (ATCC 25922), and Pseudomonas aeruginosa (ATCC 14210) strains. Clinical isolates of A. baumannii and P. aeruginosa were also tested in this study [79]. In contrast, fungal strains included Candida albicans (ATCC 10231) and Cryptococcus neoformans (kindly provided by Dr. Karen Bartlet, University of British Columbia, Vancouver, BC, Canada). Bacteria were cultured in Mueller-Hinton broth (B&D) at 37 • C, and fungal strains were cultured in Sabouraud broth (B&D) at 28 • C. In both cases, a shaker was used.

Microdilution Assay
The antimicrobial assay was conducted as in previously published protocols [80]. Shortly, in a 96-well plate, a microdilution assay was performed to determine the minimum inhibitory concentration (MIC). In 100 µL/well of Mueller-Hinton or Sabouraud broth, the following concentrations of K. fedtschenkoi extracts were tested against different microbial inocula: 50, 100, 150, and 200 µg/mL. The inoculum was prepared to have a final optical density of 0.05 at 600 nm. Bacteria treated with amikacin and gentamicin were used as positive controls, whereas untreated cells and DMSO were used as negative controls. For fungi, amphotericin and terbinafine were used as positive controls. All experiments were executed in triplicate.

Cytotoxicity Assay
The cytotoxicity of 50, 100, 150, and 200 µg/mL K. fedtschenkoi extracts was evaluated using human-derived THP-1 monocytic cells (ATCC TIB-202), following published protocols [81]. Using RPMI 1640 (Hyclone, GE Healthcare, Logan, UT, USA) supplemented with 5% fetal bovine serum (FCS) (Hyclone) and 2 mmol L −1 L-glutamine (Stem Cell Technologies, Vancouver, BC, Canada), THP-1 cells were cultured. To differentiate these cells, 20 ng/mL phorbol 12-myristate 13-acetate (PMA) was used. Then, cells were dispensed in a 96-well plate with a final volume of 100 µL at a final concentration of 1 × 10 5 cells per well. The plate was incubated at 37 • C with 5% CO 2 for 24 h. The next day, the medium was removed and replaced with fresh medium, and K. fedtschenkoi extracts at concentrations mentioned above. Again, the plate was incubated at 37 • C supplemented with 5% CO 2 for 24 h. Untreated cells and DMSO served as negative controls. Cells treated with 2% Tween-20 were used as a positive control. According to the same protocol [81], the cytotoxicity of K. fedtschenkoi extracts was known by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The next day, 2 h prior to the end of the incubation period, 25 µL of a working solution of MTT (5 mg mL −1 ) was added to the cells, and they were incubated for a further 4 h. To dissolve formazan, 100 µL of extraction buffer was added per well; the plate was incubated overnight at 37 • C. In a plate reader, readings were performed at 570 nm. The half-maximal inhibitory concentration (IC 50 ) was estimated by plotting the log concentrations of the extracts against the percentage of damaged cells. All experiments were performed in triplicate.

Anti-Inflammatory Assay
To study the anti-inflammatory activity of K. fedtschenkoi extracts, published protocols were followed [81]. In brief, THP-1 cells differentiated with PMA were used in a 96-well plate at a final concentration of 1 × 10 5 cells per well. Given the results obtained during the cytotoxicity assay, K. fedtschenkoi extracts were assayed at a final concentration of 50 µg/mL. Cells treated with 100 ng/mL of lipopolysaccharide (LPS) from E. coli (Sigma-Aldrich, St. Louis, MO, USA) were used as a positive control. Contrarily, cells treated with DMSO were used as a negative control. The final concentration of DMSO per well was always ≤1%. The measurement of the pro-inflammatory cytokine IL-6 and the anti-inflammatory cytokine IL-10 was executed with commercial kits (B&D) following instructions from the manufacturer. Readings were recorded using a plate reader at 450 nm. All experiments were carried out in triplicate.

FTIR Analysis
The chemical composition of K. fedtschenkoi extracts was studied using a Cary 630 Fourier-transform infrared (FTIR) spectrometer (Agilent Technologies, Santa Clara, CA, USA). Briefly, the detection diamond was cleaned with 10 µL of ethanol solution (100% v/v) and allowed to air dry before analyses. Background spectra were recorded without samples at room temperature. For sample analysis, 20 mg of each extract was used per reading, and the crystal was cleaned after each measurement. Measurements were recorded within the 4000 to 400 cm −1 wavenumber region. All readings were recorded in triplicate.

Statistical Analysis
Data from the quantitative viability analysis were subjected to two-way analysis of variance (ANOVA), followed by a Tukey's mean separation test, to determine the relationship between each treatment using OriginPro 2023 data processing software (OriginLab, Northampton, MA, USA).

Conclusions
This study demonstrated that K. fedtschenkoi chloroform and methanol extract exhibit antibacterial activity against multidrug-resistant bacteria such as P. aeruginosa and MRSA.
During the cytotoxicity assay, the three extracts were highly cytotoxic against THP-1 cells with IC 50 < 3 µg/mL, which suggests their potential use against cancer in in vitro or in vivo models. Furthermore, statistical analysis revealed that methanol extract exhibited higher cytotoxicity (p < 0.0001) against the tested cell line than hexane and chloroform extract. On the other hand, treatment with K. fedtschenkoi chloroform extract promoted the secretion of the anti-inflammatory cytokine IL-10.
Regarding their phytochemical content, this work demonstrated that hexane and chloroform extracts are mainly comprised of fatty acids, sterols, and triterpenes. In contrast, the derivatives of flavonoids such as quercetin and kaempferol are mainly in the methanol extract. In addition, we determined that methanol extract is abundant in flavonoids, as it presented a TFC of 384.54 ± 2.25 mg Qu/g. Using FTIR spectroscopy, we also demonstrated that extracts from the leaves of K. fedtschenkoi contain several functional groups such as C-H, C-O, C=O, C=C, and OH groups that might be related to compounds identified during the GC/MS or HPLC analyses performed in this work.
To the best of our knowledge, this is the first report that details the antimicrobial, cytotoxic, and anti-inflammatory properties of non-polar and polar extracts from K. fedtschenkoi. In addition, this study expands upon the knowledge on the genus Kalanchoe and assesses the importance of continuing to explore its therapeutic potential.
Supplementary Materials: The following supporting information can be downloaded at: https://www. mdpi.com/article/10.3390/plants12101943/s1, Figure S1: GC/MS chromatogram of hexane extract from K. fedtschenkoi. Figure S2: GC/MS chromatogram of chloroform extract from K. fedtschenkoi. Figure S3: HPLC chromatogram of methanol extract from K. fedtschenkoi. Figure   Data Availability Statement: The information generated in this study can be consulted with authors for correspondence from this work.