Muscular Oxygen Saturation and Hemoglobin Concentration during Freediving: A Case Study

: Lower limb muscle fatigue is the main reason for withdrawal from diving. Therefore, this study aimed to determine the local muscle oxygen saturation and hemoglobin concentration in the vastus lateralis muscle during different freediving disciplines. One freediver participated in this study, and his chronological age was 40 years, body mass 75.0 kg, body height 184.0 cm, and body fat 13.7%. The participant has been practicing freediving for 6 years. The variables in this study included anthropometric indices, heart rate, and muscle oxygen dynamics parameters (SmO 2 (oxygen muscle saturation) and tHb (total hemoglobin)). The variables were measured during five diving disciplines: static apnea, bifin, dynamic no fins (DNF), monofin, and sneaking. Measurements were performed during intensive training/competition during the diving season in August 2023. The results of this study showed that when oxygen starts to decrease during the dive, the tHb increases. Furthermore, the times at which maximal tHb and minimal SmO 2 were achieved are also shown. These parameters occurred at almost the same time across all disciplines: static (SmO 2 , 142; tHb, 150 s), bifin (SmO 2 , 153; tHb, 148 s), DNF (SmO 2 , 162; tHb, 178 s), monofin (SmO 2 , 96; tHb, 94 s), and sneaking (SmO 2 , 212; tHb, 228 s). Also, differences in tHb and SmO 2 were present between diving disciplines. In particular, the highest increase in tHb was present in bifin (0.0028 AU/s), whereas monofin showed a decrease ( − 0.0009 AU/s). On the other hand, the highest desaturation was seen in bifin ( − 0.87%/s) and the lowest in sneaking ( − 0.29%/s) These findings emphasize the physiological characteristics of freedivers engaging in different freediving disciplines that influence muscles during the dive. Such responses could be observed through a concurrent hypoxia/hypercapnia and a transient reduction in the Fahraeus effect.


Introduction
Freediving is an extreme sport that places high physiological demands on athletes during their dives [1,2].All freediving disciplines fit into the category of breath-holding or breath-hold diving (BHD).BHD is defined as resistance to a low oxygen environment, a reduction in sensitivity to hypoxia, and low mitochondrial oxygen consumption [3].Presently, BHD is performed by many different participants (e.g., recreational divers, seafood divers, military divers, and competitive athletes) [4,5].The periods of apnea that participants endure are why freediving is an extreme sport and why freedivers face potential danger [6,7].In competitive BHD, the freedivers are constantly trying to break records in all disciplines, despite the possible dangers.Previously, several performance factors in freediving have been defined: the amount of oxygen that is stored in the body (e.g., lungs, blood, and tissues), tolerance of cerebral ischaemia, metabolic capacity, and the level of activation of the diving response [8].
Oxygen 2024, 4 286 Apart from these factors, all participants in BHD have some physiological responses, such as the diving response (DR), which comprises reduced cardiac output (CO), bradycardia, and skeletal muscle ischaemia [9][10][11][12][13].Specifically, the DR is initiated during apnea or facial submersion in water and includes peripheral vasoconstriction, reduced CO, bradycardia, and low muscle oxygenation, and cerebral perfusion is augmented as hypercapnia develops [9][10][11][12][13][14].Furthermore, the appropriate mechanics and technique regarding movement are also necessary.In addition, muscle ischaemia and acidosis during the dive could also be seen as important parameters of performance [13].These parameters may lead to a loss of muscle strength, which is important for the propulsion of the fins during dynamic dives.
Near-infrared spectroscopy (NIRS) is a non-invasive assessment tool for viewing changes in oxygenation and hemodynamics in skeletal muscle tissue [15,16].NIRS measures blood volume, muscle oxygenation, and muscle oxygen saturation (SmO 2 ), which represents oxygen delivery to the precise muscle area beneath the sensor during exercise [17].Moreover, continuous-wave NIRS does not provide absolute measurements; therefore, only relative NIRS blood volume measures were reported.SmO 2 , which has a value range of 1% to 100%, precisely represents the dynamic equilibrium between the supply and consumption of oxygen in muscle tissue and is unaffected by the path of the near-infrared photons (the algorithm was developed to solve the problem of the unknown differential path length factor) [18].Apart from SmO 2 , NIRS could determine the fluctuation of hemoglobin in the measured region by measuring total hemoglobin (tHb).tHb is a parameter estimated from hemoglobin concentration in the blood and myoglobin in the muscle, and it indirectly shows hemodynamics or changes in local muscle blood volume [19].Previously, NIRS variables were measured in the right vastus lateralis muscle of the freedivers, which is the most common site for the examination of local muscle oxygenation dynamics [20].So far, there is sparse evidence of NIRS usage in freediving, especially in specific conditions (i.e., diving disciplines).Prior research on several bodily sites (particularly cerebral) defined NIRS measures in freediving [21].The primary goals of these investigations were to ascertain and comprehend human physiology in low-oxygen settings [11,22].Also, some NIRS studies were performed using laboratory tests that included non-specific procedures [20,23].Following that, there is a need for an examination of NIRS parameters during specific freediving disciplines.This is mainly because of fatigue in the lower limb muscles after prolonged dives.Therefore, this study aimed to determine the local muscle oxygen saturation and hemoglobin concentration in the right vastus lateralis muscle of freedivers during different freediving disciplines.Also, the secondary aim was to observe SmO 2 and tHb parameters in relation to heart rate.

Participant
One freediver participated in this study, and his chronological age was 40 years, body mass 75.0 kg, body height 184.0 cm, and body fat 13.7%.The participant has been practicing freediving for 6 years, and he could be considered as a moderate athlete.Measurements were performed during intensive training/competition during the diving season in August 2023.He was informed about the procedures and purpose of the study and provided informed consent before the investigation began.Before the beginning of the procedure, the participant was informed about all the risks and possible dangers of the procedure and provided signed written consent.All experimental procedures were completed according to the Declaration of Helsinki, and they were approved by the corresponding author's institutional research ethics board (ethics board approval no.2181-205-02-05-22-035).

Variables
The variables in this study included anthropometric indices, heart rate, blood lactate levels, and muscle oxygen dynamics parameters (SmO 2 and tHb).
Anthropometric indices included body height, body mass, and body fat percentage.Body mass and body fat percentage were assessed with the bioimpedance scale (Tanita BC 418 scale; Tokyo, Japan).Body height was determined with a Tanita HR-001 anthropometer (Tanita; Tokyo, Japan).Heart rate was assessed with a Garmin Descent Mk1 watch-sized dive computer (Garmin Ltd., Olathe, KS, USA).Lactate levels were analyzed after every discipline of freediving.The analysis was performed by professional personnel using the lactate Accutrend analyzer (Roche, Boehringer Mannheim, Indianapolis, IN, USA).
Muscle oxygenation and blood volume changes in the lower limb muscles were measured by the commercially available continuous wave NIRS sensor (Moxy Monitor; Fortiori Design LLC, Hutchinson, MN, USA).The functioning, usage, validity, and reliability of the NIRS sensor have been previously described [18,24].The main parameter generated by the NIRS sensor is SmO 2 , which is calculated as a ratio of relative oxyhemoglobin to oxymyoglobin and relative deoxyhemoglobin to deoxymyoglobin [18].Apart from SmO 2 , tHb (total blood hemoglobin) is also a parameter derived from the NIRS sensor readings and indicates changes in blood volume in a particular muscle under the sensor [15].Specifically, tHb is a parameter estimated from hemoglobin and myoglobin concentration in the blood, and it indirectly shows hemodynamics or changes in local muscle blood volume [19].
Additionally, the following muscle oxygen parameters were calculated: baseline SmO 2 (average muscle oxygen saturation before the test), initial desaturation SmO 2 rate (desat slope) (inclination of the slope at the beginning of the test to first breakpoint in the curve), minimal SmO 2 (minimal oxygen saturation during the dive), and maximal SmO 2 (maximal oxygen saturation during recovery).tHb measurements included baseline tHb (average muscle oxygen saturation before the test), maximal tHb (maximal hemoglobin during the test), minimal tHb (minimal hemoglobin before the test), and tHb slope (inclination of the slope at the beginning of the test to first breakpoint in the curve).Furthermore, breakpoints were determined via least squares piecewise regression analysis at the minimally attainable SmO 2 [24] (see Figure 1).
Anthropometric indices included body height, body mass, and body fat percentage.Body mass and body fat percentage were assessed with the bioimpedance scale (Tanita BC 418 scale; Tokyo, Japan).Body height was determined with a Tanita HR-001 anthropometer (Tanita; Tokyo, Japan).Heart rate was assessed with a Garmin Descent Mk1 watch-sized dive computer (Garmin Ltd., Olathe, KS, USA).Lactate levels were analyzed after every discipline of freediving.The analysis was performed by professional personnel using the lactate Accutrend analyzer (Roche, Boehringer Mannheim, Indianapolis, IN, USA).
Muscle oxygenation and blood volume changes in the lower limb muscles were measured by the commercially available continuous wave NIRS sensor (Moxy Monitor; Fortiori Design LLC, Hutchinson, MN, USA).The functioning, usage, validity, and reliability of the NIRS sensor have been previously described [18,24].The main parameter generated by the NIRS sensor is SmO2, which is calculated as a ratio of relative oxyhemoglobin to oxymyoglobin and relative deoxyhemoglobin to deoxymyoglobin [18].Apart from SmO2, tHb (total blood hemoglobin) is also a parameter derived from the NIRS sensor readings and indicates changes in blood volume in a particular muscle under the sensor [15].Specifically, tHb is a parameter estimated from hemoglobin and myoglobin concentration in the blood, and it indirectly shows hemodynamics or changes in local muscle blood volume [19].
Additionally, the following muscle oxygen parameters were calculated: baseline SmO2 (average muscle oxygen saturation before the test), initial desaturation SmO2 rate (desat slope) (inclination of the slope at the beginning of the test to first breakpoint in the curve), minimal SmO2 (minimal oxygen saturation during the dive), and maximal SmO2 (maximal oxygen saturation during recovery).tHb measurements included baseline tHb (average muscle oxygen saturation before the test), maximal tHb (maximal hemoglobin during the test), minimal tHb (minimal hemoglobin before the test), and tHb slope (inclination of the slope at the beginning of the test to first breakpoint in the curve).Furthermore, breakpoints were determined via least squares piecewise regression analysis at the minimally attainable SmO2 [24] (see Figure 1).

Procedure
A NIRS sensor was placed over the right vastus lateralis muscle, consistent with previous studies [25,26].This muscle was used mainly because it is at the highest contraction during the tests.The oxygenation of the muscle was measured, and the appropriate light shield was placed over the NIRS sensor to prevent ambient light intrusion and interference with the sensor reading.Furthermore, the Moxy probe is waterproof and does not need any additional protection.The sensor was fixed in place with 3M medical tape.The device uses four wavelengths (680, 720, 760, and 800 nm) to assess the ratio of oxyhemoglobin to oxymyoglobin and deoxyhemoglobin to deoxymyoglobin via a modified Beer-Lambert equation, resulting in a percent concentration of SmO 2 .The device detectors were spaced 12.5 mm and 25 mm from the emitter.The sampling rate was set using the default mode, which samples the four wavelengths over 80 cycles to generate an average output every two seconds (0.5 Hz).The data were gathered using the Moxy Portal app, which provides a data export feature in xlsx format.The participant's body fat percentage was low (13.7%), and therefore, adipose tissue thickness was considered negligible for the NIRS results.
The procedure was conducted in a swimming pool during the participant's anaerobic apnea training period.He engaged in 5 disciplines of freediving training (static apnea, bifin, dynamic no fins (DNF), monofin, and sneaking), one after another.Static apnea was performed on dry land while the athlete was holding his breath in a supine position.The bifin and monofin disciplines were performed in the swimming pool with the use of two fins and one fin.DNF and sneaking were performed without additional diving equipment.Furthermore, during sneaking, the athlete slowly crawled on the floor of the swimming pool.All disciplines were submaximal for the participant.
During the intense diving training/competition season in August 2023, local muscle oxygenation and hemodynamics were measured together with heart rate.After each discipline, the diver rested for at least 10 min to fully recover so that the measured parameters could return to their baseline values.The NIRS data acquisition was performed for 2 min before the start of the dive and 2 min after the dive was performed, and heart rate was measured in the same manner (see Figure 2).

Procedure
A NIRS sensor was placed over the right vastus lateralis muscle, consistent with previous studies [25,26].This muscle was used mainly because it is at the highest contraction during the tests.The oxygenation of the muscle was measured, and the appropriate light shield was placed over the NIRS sensor to prevent ambient light intrusion and interference with the sensor reading.Furthermore, the Moxy probe is waterproof and does not need any additional protection.The sensor was fixed in place with 3M medical tape.The device uses four wavelengths (680, 720, 760, and 800 nm) to assess the ratio of oxyhemoglobin to oxymyoglobin and deoxyhemoglobin to deoxymyoglobin via a modified Beer-Lambert equation, resulting in a percent concentration of SmO2.The device detectors were spaced 12.5 mm and 25 mm from the emitter.The sampling rate was set using the default mode, which samples the four wavelengths over 80 cycles to generate an average output every two seconds (0.5 Hz).The data were gathered using the Moxy Portal app, which provides a data export feature in xlsx format.The participant's body fat percentage was low (13.7%), and therefore, adipose tissue thickness was considered negligible for the NIRS results.
The procedure was conducted in a swimming pool during the participant's anaerobic apnea training period.He engaged in 5 disciplines of freediving training (static apnea, bifin, dynamic no fins (DNF), monofin, and sneaking), one after another.Static apnea was performed on dry land while the athlete was holding his breath in a supine position.The bifin and monofin disciplines were performed in the swimming pool with the use of two fins and one fin.DNF and sneaking were performed without additional diving equipment.Furthermore, during sneaking, the athlete slowly crawled on the floor of the swimming pool.All disciplines were submaximal for the participant.
During the intense diving training/competition season in August 2023, local muscle oxygenation and hemodynamics were measured together with heart rate.After each discipline, the diver rested for at least 10 min to fully recover so that the measured parameters could return to their baseline values.The NIRS data acquisition was performed for 2 min before the start of the dive and 2 min after the dive was performed, and heart rate was measured in the same manner (see Figure 2).

Statistical Analysis
Descriptive statistics were measured to assess the arithmetic means and standard deviations of all measured variables.The K-S (Kolmogorov-Smirnov) test for normality was used to determine the normal distribution of the data.The data in the figures are presented as normalized values with a range from 0% to 100%.The normalization was performed according to the formula below:

Statistical Analysis
Descriptive statistics were measured to assess the arithmetic means and standard deviations of all measured variables.The K-S (Kolmogorov-Smirnov) test for normality was used to determine the normal distribution of the data.The data in the figures are presented as normalized values with a range from 0% to 100%.The normalization was performed according to the formula below: Statistica version 13.0 (Dell Inc., Austin, TX, USA) was used for the analyses, and a level of 95% (p < 0.05) was applied.

Results
Tables 1-3 show results of the measured variables (duration, heart rate, tHb, and SmO 2 ) in the five freediving disciplines.The static and sneaking disciplines were the longest in duration at 218.0 s and 165.0 s, respectively.Furthermore, the participant experienced a decrease in heart rate during all disciplines.On the other hand, the highest heart rate decrease was observed in the static (−0.33 bpm/s) and DNF (−0.31 bpm/s) disciplines (see Table 1).The tHb results showed an increased trend in all disciplines, except monofin (−0.0009AU/s).The largest increase was seen in bifin (0.0028 AU/s) (see Table 2).Table 3 shows the SmO 2 variables (baseline SmO 2 %, desaturation slope, minimal SmO 2 %, and maximal SmO 2 %).The results showed that the hemoglobin in the vastus lateralis muscle desaturated the fastest in bifin (−0.87%/s) and the slowest in sneaking (−0.29%/s).
Figure 3 presents tHb and SmO 2 trends in all diving disciplines.The analysis showed that when oxygen started to decrease during the dive, the tHb increased.By contrast, after the dive, the SmO 2 % increased and the tHb decreased.Furthermore, the times at which the maximal tHb and the minimal SmO 2 were reached are also shown.These parameters occurred at almost the same time across all disciplines: static (SmO 2 , 142 s; tHb, 150 s), bifin (SmO 2 , 153 s; tHb, 148 s), DNF (SmO 2 , 162 s; tHb, 178 s), monofin (SmO 2 , 96 s; tHb, 94 s), and sneaking (SmO 2 , 212 s; tHb, 228 s).

Discussion
Different freediving disciplines place different demands on athletes.The fatigue and acidosis of lower limb muscles are the number one reason for stopping the apnea during dives.Such extreme demands placed on athletes require an examination of local muscle hemodynamics during diving to enhance athletes' performance.Therefore, this study After the diver performed the dive, his lactate levels ranged from 1.6 to 7.7 mmol/L (static, 1.6 mmol/L; bifin, 7.6 mmol/L; DNF, 7.7 mmol/L; monofin, 6.4 mmol/L; and sneaking, 6.7 mmol/L).

Discussion
Different freediving disciplines place different demands on athletes.The fatigue and acidosis of lower limb muscles are the number one reason for stopping the apnea during dives.Such extreme demands placed on athletes require an examination of local muscle hemodynamics during diving to enhance athletes' performance.Therefore, this study aimed to examine both tHb and SmO 2 fluctuations and relations during freediving in the right vastus lateralis muscle during the intense training/competition season in August 2023.The main findings of this study are that SmO 2 and tHb have opposing trends during different diving disciplines.
As expected, the results showed that tHb and SmO 2 demonstrated opposing trends during all disciplines since extreme skeletal muscle ischemia occurs during a dive in which divers swim after taking a single breath; at the same time, muscle hyperemia (increased muscle blood flow) compensates for the local lack of oxygen during dynamic exercise.In particular, the decrease in SmO 2 corresponded with an increase in tHb.Additionally, tHb is derived as the sum of oxygenated (O 2 Hb) and deoxygenated hemoglobin (HHb) in the measured region (tHb = O 2 Hb + HHb), and it indicates a change in the blood flow to the muscle [27].On the other hand, SmO 2 is the fraction of O 2 Hb compared to tHb (SmO 2 = O 2 Hb/tHb) and is defined as the percentage of oxygenated hemoglobin and myoglobin relative to the total quantity of chromophores present in the muscle tissue at a certain moment [18].However, current technology does not allow us to determine if there are increases in O 2 Hb or HHb (deoxyHb).Therefore, these muscle oxygenation trends should be explained with a comprehensive physiological evaluation.
The trend observed regarding the increase in blood flow could be seen as hyperemic reactivity of a muscle due to extreme local hypoxia/hypercapnia.According to Alvares, et al. [28], tHb has a strong correlation with Doppler ultrasound.Additionally, the authors demonstrated that there was an increase in tHb and blood flow post exercise due to hyperemia of the muscle tissue.These findings were explained by (i) increased cardiac output (CO) (with concomitant redirection of blood flow to the working muscle) and vasodilation of downstream resistance arterioles brought about by concurrent hypoxia/hypercapnia, which typically occurs during muscle contraction and (ii) a transient reduction in the Fahraeus effect (i.e., a decrease in the hematocrit level as blood flows from larger to smaller vessels) [29].It was previously established that during freediving, divers experience involuntary breathing movements (IBM) that increase SV to maintain CO and increase blood flow [11].Therefore, the increase in tHb in our study could be connected with the renormalization of CO and the need for blood flow maintenance of working muscles.
However, the increase also occured during static apnea (SA).Therefore, other factors may have influenced the opposing trend of SmO 2 and tHb.During diving, oxygen delivery is limited to one breath that is taken before the dive.The oxygen consumption during this single breath limits the quantity of O 2 in the tissue, causing extreme local hypoxia and accumulation of CO 2 .Molchanova, et al. [30] reported that the partial pressure of CO 2 increases during prolonged dives.As a result, the increase in blood flow during both static and dynamic disciplines may be connected to the need for the removal of accumulated CO 2 .
Further data analysis showed that minimal SmO 2 and maximal tHb occurred at almost the same time, with differences ranging from 2 s to 16 s.Previously, it was demonstrated that tHb usually drops with SmO 2 , after which it increases rapidly [28].However, our study showed the opposite results, with minimal and maximal values occurring at similar times.Furthermore, this correspondence of SmO 2 and tHb values happened during different periods of the dives (i.e., beginning, middle, or end).Such results most likely occurred because of the demands that freediving places on athletes.Also, the variability in the timing most likely happened because of differences in the execution of the different dives.Specifically, different diving disciplines require different techniques concerning the use of lower limb muscles.Furthermore, differences between active and passive dives play a key role in the explanation of the deoxygenation trend.It should be noted that stronger deoxygenation trends and lower SmO 2 values were observed during passive dives (i.e., sneaking and static apnea).
There are a limited number of studies that assess the cardiovascular responses to SA and DA in pools by measuring underwater hemodynamic physiological parameters such as ECG (electrocardiogram), arterial oxygen saturation (SaO 2 ), SV, HR, and CO [9,31,32].Kiviniemi, Breskovic, Uglesic, Kuch, Maslov, Sieber, Seppänen, Tulppo and Dujic [31] investigated heart rate variability changes in the settings of SA and DA.They concluded that apnea blunts the effects of exercise on cardiac vagal activity at the end of DA; however, a higher HR during DA compared with SA indicates higher cardiac sympathetic activity during DA.Breskovic, Uglesic, Zubin, Kuch, Kraljevic, Zanchi, Ljubkovic, Sieber and Dujic [9] found moderate increases in blood pressure (BP) during SA and DA as well as a faster desaturation during DA vs. SA.These cardiovascular changes during immersed SA and DA are in agreement with those reported for dry SA and DA.Tocco, Marongiu, Pinna, Roberto, Pusceddu, Angius, Migliaccio, Milia, Concu and Crisafulli [32] also found a decrement in SV (−8%) and CO (−25%), but mean blood pressure (MBP) was maintained because of an increase in stroke volume rate (SVR) in SA.Sympathetic activation was induced by exercise during DA, which partially obscured the effects of the diving response.
Additionally, different disciplines place different demands on freedivers, which is evident from observations of SmO 2 and tHb values.Based on these results, there is an implication that bifin is the most oxygen demanding since desaturation occurs more rapidly during this discipline compared to the other disciplines.Also, the rate of tHb increase was highest during bifin, indicating a large blood flow increase in the measured muscle.Such a conclusion is logical since divers use both legs simultaneously.Furthermore, there was a similar yet smaller increasing/decreasing trend during the static disciplines (static and sneaking).The slower desaturation rate during sneaking is mostly due to the diving response that occurs in water, whereas dry-land apnea results in faster desaturation.Moreover, it should be noted that both static and dynamic apneas influence the SmO 2 decrease differently.Such an indication was seen with the relation between apnea duration and minimal SmO 2 .The results indicate that more prolonged breath holds will induce lower values of SmO 2 .Such trends and differences between disciplines could potentially implicate diving limiting factors.Specifically, more static disciplines may have less of an impact on skeletal muscle deoxygenation, and other factors may influence the duration of the dive.On the other hand, active dives lead to a higher work rate of skeletal muscle, and skeletal muscle deoxygenation may influence the cessation of the dive.Hence, different disciplines place different demands on athletes in terms of the usage of skeletal muscles, which are important to the duration of dives.Also, these deoxygenation trends and apnea breakpoints may be detrimental to performance in dynamic diving disciplines.

Limitations and Strengths
The main limitation of this study is the sample of participants, which is one athlete.Also, the intensity of the study procedure could influence the observed results.In particular, performing multiple disciplines one after the other could be seen as intensive and could have longer-lasting residual effects on the subsequent disciplines.Furthermore, the inclusion of measurements of other physiological parameters could more precisely explain SmO 2 and tHb trends in future studies.On the other hand, this study is the first one that explains these results in specific freediving disciplines.Also, the usage of NIRS underwater is lacking in the literature, apart from studies including cerebral measurements [21].Therefore, these are the main strengths of the study.

Conclusions
Conclusively, this study aimed to determine the local muscle oxygen saturation and hemoglobin concentration during freediving disciplines.According to the aims, the results of this study demonstrated opposing SmO 2 and tHb trends.Furthermore, the minimal SmO 2 and maximal tHb occurred almost simultaneously during the dive.Additionally, the trends in the rates of increase/decrease in SmO 2 and tHb were different during the different disciplines.
These findings outline the physiological characteristics of freediving that influence muscles during the dive.Such responses may have been observed because of a concurrent extreme local hypoxia/hypercapnia and a transient reduction in the Fahraeus effect.Furthermore, there were different trends in the timing of low oxygen and high blood flow during the different disciplines.These results could be used in freediving training to monitor the safety of the divers and to influence the resistance to hypoxia/hypercapnia, resulting in an increase in the duration of dives.

Figure 1 .
Figure 1.A graphical example of the time course of SmO 2 (black line) and tHb (grey line), with variables (baseline, slope, minimal, and maximal) gathered from the right vastus lateralis muscle.

Figure 1 .
Figure 1.A graphical example of the time course of SmO2 (black line) and tHb (grey line), with variables (baseline, slope, minimal, and maximal) gathered from the right vastus lateralis muscle.

Figure 2 .
Figure 2. Data collection timeline with the order of the tested disciplines.

Figure 2 .
Figure 2. Data collection timeline with the order of the tested disciplines.

Figure 3 .
Figure 3.A time course of normalized SmO2 (black line) and tHb (grey line) variables gathered from the right vastus lateralis muscle during rest, test, and rest in (A) static, (B) bifin, (C) DNF, (D) monofin, and (E) sneaking disciplines; times of minimal SmO2 (dotted line) and maximal tHb (dashed line) are shown.

Figure 3 .
Figure 3.A time course of normalized SmO 2 (black line) and tHb (grey line) variables gathered from the right vastus lateralis muscle during rest, test, and rest in (A) static, (B) bifin, (C) DNF, (D) monofin, and (E) sneaking disciplines; times of minimal SmO 2 (dotted line) and maximal tHb (dashed line) are shown.

Table 1 .
Results of apnea duration and heart rate parameters in all measured disciplines.

Table 2 .
Results of total hemoglobin parameters (tHb) in all measured disciplines.

Table 3 .
Results of oxygen saturation parameters (SmO 2 ) in all measured disciplines.