Thermal-Recoverable Tough Hydrogels Enhanced by Porphyrin Decorated Graphene Oxide

Artificial tissue materials usually suffer properties and structure loss over time. As a usual strategy, a new substitution is required to replace the worn one to maintain the functions. Although several approaches have been developed to restore the mechanical properties of hydrogels, they require direct heating or touching, which cannot be processed within the body. In this manuscript, a photothermal method was developed to restore the mechanical properties of the tough hydrogels by using near infrared (NIR) laser irradiation. By adding the porphyrin decorated graphene oxide (PGO) as the nanoreinforcer and photothermal agent into carrageenan/polyacrylamide double network hydrogels (PDN), the compressive strength of the PDN was greatly improved by 104%. Under a short time of NIR laser irradiation, the PGO effectively converts light energy to thermal energy to heat the PDN hydrogels. The damaged carrageenan network was rebuilt, and a 90% compressive strength recovery was achieved. The PGO not only significantly improves the mechanical performance of PDN, but also restores the compressive property of PDN via a photothermal method. These tough hydrogels with superior photothermal recovery may work as promising substitutes for load-bearing tissues.


Mechanical Recovery of Hydrogels
The thermal recovery process is implemented as follows: after compression, hydrogels were heated at 60 °C for 5 min and cooled to room temperature. After thermal recovery, the compressive properties of hydrogels were tested again by unconfined compression. In addition, a NIR Laser generator (Dragon Lasers, Changchun, China) was used to provide NIR light. The heating rates of Control, GDN, and PDN were measured for 30 s using a thermometer. The photothermal recovery process was implemented as follows: after compression, hydrogels were heated under an 808 nm laser generator ( for 10 min and cooled down to room temperature. After that, the compressive properties of hydrogels were tested again by unconfined compression. The temperature changes of DN and PDN gels were also measured by a FLIR SC8303 high-speed infrared camera. The camera was placed directly in front of DN and PDN hydrogels, recording two-dimensional transient temperature distribution images. The viewing area of the samples and holder was set at a 640 × 360 pixel window frame size, with an acquisition rate of 2 frames per second.

Hydrogels Fabrication
The PGO reinforced carrageenan/polyacrylamide (carr/PAAm) DN (PDN) hydrogels were fabricated via a two-step polymerization method [42]. After 400 mg of carrageen kappa, 2400 mg of AAm, 6 mg of MBAA (0.0025 of AAm) were dissolved into 10 mL 0.1 mg/mL PGO solution with 90 • C oil bath, and the mixture solution was injected into Teflon molds. After cooling down in the refrigerator overnight, the formed gels were immersed into 30 mL DI water with 7200 mg of AAm, 36 mg of the MBAA, and 216 mg of APS for 24 h. After removing the soft PAAm single network (SN) hydrogels, the PDN hydrogels were achieved for the following characterizations. The carr/PAAm DN hydrogels and GO reinforced carr/PAAm DN (GDN) hydrogels were prepared via following the same procedure with pure DI water and 0.1 mg/mL GO solution.

Mechanical Recovery of Hydrogels
The thermal recovery process is implemented as follows: after compression, hydrogels were heated at 60 • C for 5 min and cooled to room temperature. After thermal recovery, the compressive properties of hydrogels were tested again by unconfined compression. In addition, a NIR Laser generator (Dragon Lasers, Changchun, China) was used to provide NIR light. The heating rates of Control, GDN, and PDN were measured for 30 s using a thermometer. The photothermal recovery process was implemented as follows: after compression, hydrogels were heated under an 808 nm laser generator (for 10 min and cooled down to room temperature. After that, the compressive properties of hydrogels were tested again by unconfined compression. The temperature changes of DN and PDN gels were also measured by a FLIR SC8303 high-speed infrared camera. The camera was placed directly in front of DN and PDN hydrogels, recording two-dimensional transient temperature distribution images. The viewing area of the samples and holder was set at a 640 × 360 pixel window frame size, with an acquisition rate of 2 frames per second.

Materials Characterization
The morphology of the PGO and GO was investigated by transmission electron microscopy (TEM, HITACHI 8100, Hitachi Appliances Inc., Tokyo, Japan, and the composition of the PGO, GO, and porphyrin was tested by Fourier transform infrared spectroscopy (FTIR, Nicolet IS10 FTIR spectrometer from Thermo Scientific, Waltham, MA, USA), thermogravimetric analysis (TGA, Q50 from TA Instruments Inc., New Castle, DE, USA) and UV-vis spectrum (InfiniteM1000 Pro plate reader from Tecan, Männedorf, Switzerland). The gel-sol transition temperature of carrageen was obtained by differential scanning calorimetry (DSC, Q20 from TA Instruments Inc., New Castle, DE, USA).

Compressive Measurement
The mechanical properties of the hydrogels were investigated by unconfined compression using a commercial machine (AGS-X from SHIMADZU, Kyoto, Japan). An Instron 5966 (Instron Corporation, Canton, MA, USA) was used to measure cyclic compressive properties of hydrogels, and the compressive velocity was 600 µm/min. The compressive strain Eis defined as: where h is the height during deformation, and h 0 is the initial height of the gel sample. The normal compressive stress σ normal is defined as: where F is the applied load, and A 0 is the original top surface area of samples. The fatigue resistance of the DN and TN gels was characterized by a successive loading-unloading test. Three increasing compressive strains (30%, 60%, and 90%) were used in this experiment. The compressive toughness (U) is defined as: where F is the applied force, and s is the corresponding displacement. The hysteresis energy (U hyst ) is calculated as

Swelling Measurement
The swelling ratio (SR) was defined as: where Ws and Wo represent the weight of hydrogels after swelling in DI water in different time periods and the weight of hydrogels before swelling, respectively.

Porphyrin-Graphene Oxide (PGO)
The attachment of the porphyrin on the GO was confirmed by the TEM images, TGA results, FTIR spectra, zeta potential, and UV-vis spectra. TEM images showed the few-layer structures of GO ( Figure 1a) and PGO (Figure 1b). Compared to clean planar GO nanosheets, a certain amount of porphyrin particle aggregations were presented on the PGO nanosheets, which clearly indicates that the GO is conjugated with porphyrin via π-π interaction. These results are similar to our previous results [40]. the GO is conjugated with porphyrin via π-π interaction. These results are similar to our previous results [40].
(a) (b) Other results, including FTIR spectra, TGA curve, and UV-vis absorption, further validate the π-π interaction between porphyrin and GO in PGO, which is consistent with the TEM results. The FTIR spectra (Figure 2a-c) indicates the existence of the porphyrin on the GO by the peaks around 750-1500 nm. Figure 2a presents that the epoxy groups (1390 cm −1 , 1060 cm −1 , and 946 cm −1 ), carboxyl group (1720 cm −1 ), and hydroxyl group (3400 cm −1 ) existed in the as-prepared GO. However, the peaks (1720 cm −1 , 1390 cm −1 , 1060 cm −1 , and 946 cm −1 ) were covered by peaks of porphyrin, and the peak of the hydroxyl group (3400 cm −1 ) did exist (Figure 2c), which demonstrates that the porphyrin was successfully combined with GO. Figure 2d-f present the TGA results of the GO, porphyrin, and PGO. Clear weight reductions around 180 °C and 400 °C were presented in GO and porphyrin, respectively, which were corresponding to the decomposition temperature of GO and porphyrin. As depicted in Figure 2f, two obvious weight cutbacks were found around 180 °C and 400 °C, which indicates that the porphyrin was attached to GO. A similar conclusion was presented in the UV-vis spectra (Figure 2g-i). No obvious peaks were shown on the spectrum of GO; however, a weak peak, called the Soret band, appeared around 420 nm on the spectrum of porphyrin, which was a result of poor solubility of porphyrin in DI water. A strong peak at the same position (420 nm) was also observed in the spectrum of PGO, which demonstrates that the porphyrin was profitably connected onto GO.
In addition, the average zeta potential of GO and PGO was around −55 mV and −25 mV, respectively ( Figure S1). A slight decrease was found after GO conjugated with porphyrin, indicating that the stability of PGO resembles GO, which is also consistent with our previous results [40]. Other results, including FTIR spectra, TGA curve, and UV-vis absorption, further validate the π-π interaction between porphyrin and GO in PGO, which is consistent with the TEM results. The FTIR spectra (Figure 2a-c) indicates the existence of the porphyrin on the GO by the peaks around 750-1500 nm. Figure 2a presents that the epoxy groups (1390 cm −1 , 1060 cm −1 , and 946 cm −1 ), carboxyl group (1720 cm −1 ), and hydroxyl group (3400 cm −1 ) existed in the as-prepared GO. However, the peaks (1720 cm −1 , 1390 cm −1 , 1060 cm −1 , and 946 cm −1 ) were covered by peaks of porphyrin, and the peak of the hydroxyl group (3400 cm −1 ) did exist (Figure 2c), which demonstrates that the porphyrin was successfully combined with GO. Figure 2d-f present the TGA results of the GO, porphyrin, and PGO. Clear weight reductions around 180 • C and 400 • C were presented in GO and porphyrin, respectively, which were corresponding to the decomposition temperature of GO and porphyrin. As depicted in Figure 2f, two obvious weight cutbacks were found around 180 • C and 400 • C, which indicates that the porphyrin was attached to GO. A similar conclusion was presented in the UV-vis spectra (Figure 2g-i). No obvious peaks were shown on the spectrum of GO; however, a weak peak, called the Soret band, appeared around 420 nm on the spectrum of porphyrin, which was a result of poor solubility of porphyrin in DI water. A strong peak at the same position (420 nm) was also observed in the spectrum of PGO, which demonstrates that the porphyrin was profitably connected onto GO.
In addition, the average zeta potential of GO and PGO was around −55 mV and −25 mV, respectively ( Figure S1). A slight decrease was found after GO conjugated with porphyrin, indicating that the stability of PGO resembles GO, which is also consistent with our previous results [40].
Due to the presence of porphyrin on GO layers, it enhances the NIR adsorption and its photo-thermal conversion efficiency under NIR irradiation. As shown in Figure 3a, the temperature of DI water nearly changed. On the other hand, the 0.1 mg/mL PGO solution showed a higher temperature increase (∆T = 41.6 • C) than the 0.1 mg/mL GO solution (∆T = 21.7 • C), which suggests that 91.7% more heat was converted from NIR irradiation (808 nm, 2.5 W cm 2 , 10 min) by PGO compared to the GO solution. In addition, the temperature of the PGO solution was above 50 • C after 3 min of NIR laser irradiation; however, that of the GO solution was only 36 • C. These results demonstrate that PGO can efficiently adsorb NIR light and transfer it into heat energy. Figure 3b showed that the PGO solution owned higher adsorption in the NIR range, while the GO solution presented small adsorption, indicating that porphyrin conjugation enhances NIR absorbance of GO and porphyrin particle aggregations on GO nanosheets act as antennas to harvest thermal energy from NIR light. The insert in Figure 3b presented that a negligible change of temperature enhancement (∆T ≈ 43 • C) Nanomaterials 2019, 9, 1487 6 of 14 was found after four NIR laser ON/OFF cycles, which demonstrates the stability of PGO in aqueous solution after irradiation. Due to the presence of porphyrin on GO layers, it enhances the NIR adsorption and its photothermal conversion efficiency under NIR irradiation. As shown in Figure 3a, the temperature of DI water nearly changed. On the other hand, the 0.1 mg/mL PGO solution showed a higher temperature increase (ΔT = 41.6 °C) than the 0.1 mg/mL GO solution (ΔT = 21.7 °C), which suggests that 91.7% more heat was converted from NIR irradiation (808 nm, 2.5 W cm 2 , 10 min) by PGO compared to the GO solution. In addition, the temperature of the PGO solution was above 50 °C after 3 min of NIR laser irradiation; however, that of the GO solution was only 36 °C. These results demonstrate that PGO can efficiently adsorb NIR light and transfer it into heat energy. Figure 3b showed that the PGO solution owned higher adsorption in the NIR range, while the GO solution presented small adsorption, indicating that porphyrin conjugation enhances NIR absorbance of GO and porphyrin particle aggregations on GO nanosheets act as antennas to harvest thermal energy from NIR light. The insert in Figure 3b presented that a negligible change of temperature enhancement (ΔT ≈ 43 °C) was found after four NIR laser ON/OFF cycles, which demonstrates the stability of PGO in aqueous solution after irradiation.  Due to the presence of porphyrin on GO layers, it enhances the NIR adsorption and its photothermal conversion efficiency under NIR irradiation. As shown in Figure 3a, the temperature of DI water nearly changed. On the other hand, the 0.1 mg/mL PGO solution showed a higher temperature increase (ΔT = 41.6 °C) than the 0.1 mg/mL GO solution (ΔT = 21.7 °C), which suggests that 91.7% more heat was converted from NIR irradiation (808 nm, 2.5 W cm 2 , 10 min) by PGO compared to the GO solution. In addition, the temperature of the PGO solution was above 50 °C after 3 min of NIR laser irradiation; however, that of the GO solution was only 36 °C. These results demonstrate that PGO can efficiently adsorb NIR light and transfer it into heat energy. Figure 3b showed that the PGO solution owned higher adsorption in the NIR range, while the GO solution presented small adsorption, indicating that porphyrin conjugation enhances NIR absorbance of GO and porphyrin particle aggregations on GO nanosheets act as antennas to harvest thermal energy from NIR light. The insert in Figure 3b presented that a negligible change of temperature enhancement (ΔT ≈ 43 °C) was found after four NIR laser ON/OFF cycles, which demonstrates the stability of PGO in aqueous solution after irradiation.

PGO Reinforced Hydrogel (PDN)
The PGO reinforced DN (PDN) gels were fabricated by introducing the PGO into carr/PAAm DN hydrogels. The compressive stress-strain curves of the hydrogels were presented in Figure 4. As shown in Figure 4a and Table S1, the compressive strength was remarkably improved from 9.00 ± 0.30 MPa (DN) to 19.17 ± 0.36 MPa (GO reinforced carr/PAAm DN (GDN)) and 18.32 ± 0.3 MPa (PDN) at 99% strain, respectively. These results indicate that the introduction of GO and PGO efficiently dissipates the mechanical energy during loading, leading to a 113% and 104% improvement in compressive strength. The mechanical improvement mostly derives from the physical entanglements between soft polymeric chains and rigid two-dimensional GO nanosheets, enhancing energy adsorption during deformation. The compressive strength and toughness of GDN gels were slightly higher than that of PDN gels, which may result from hydrogen bonds between functional groups on the GO nanosheets and amine groups on the PAAm network, improving the cross-linking bonds in the GDN hydrogels.

PGO Reinforced Hydrogel (PDN)
The PGO reinforced DN (PDN) gels were fabricated by introducing the PGO into carr/PAAm DN hydrogels. The compressive stress-strain curves of the hydrogels were presented in Figure 4. As shown in Figure 4a and Table S1, the compressive strength was remarkably improved from 9.00 ± 0.30 MPa (DN) to 19.17 ± 0.36 MPa (GO reinforced carr/PAAm DN (GDN)) and 18.32 ± 0.3 MPa (PDN) at 99% strain, respectively. These results indicate that the introduction of GO and PGO efficiently dissipates the mechanical energy during loading, leading to a 113% and 104% improvement in compressive strength. The mechanical improvement mostly derives from the physical entanglements between soft polymeric chains and rigid two-dimensional GO nanosheets, enhancing energy adsorption during deformation. The compressive strength and toughness of GDN gels were slightly higher than that of PDN gels, which may result from hydrogen bonds between functional groups on the GO nanosheets and amine groups on the PAAm network, improving the cross-linking bonds in the GDN hydrogels.  The fatigue resistance of PDN gels has been systematically investigated by performing cyclic loading-unloading tests at constant strain. Figure 4b,c present the consecutive loading-unloading curves with gradient increases in the maximum strain (30%, 60%, and 90%) of DN and PDN gels, respectively. The compressive strength and hysteresis energy of PDN gels were both largely higher than that of DN gels (Table S2), which also indicates that PGO can efficiently improve the mechanical The fatigue resistance of PDN gels has been systematically investigated by performing cyclic loading-unloading tests at constant strain. Figure 4b,c present the consecutive loading-unloading curves with gradient increases in the maximum strain (30%, 60%, and 90%) of DN and PDN gels, respectively. The compressive strength and hysteresis energy of PDN gels were both largely higher than that of DN gels (Table S2), which also indicates that PGO can efficiently improve the mechanical properties of DN gels. Both DN and PDN hydrogels underwent two sequential loading-unloading cycles (30% and 60% strain), and these loading curves were mostly overlapped, which demonstrates that the carrageenan network is intact under low strain. However, the loading curves of 90% strain deviate below the previous loading curves, which indicates the carrageenan network is damaged. Figure 4d presents five immediate consecutive loading-unloading curves of PDN at 90% compression strain, while DN gels crashed into pieces after the first 90% strain compression. It demonstrates that PDN hydrogels own stronger mechanical properties than DN gels. The PDN gels showed that the hysteresis loops become smaller in subsequent loading. This phenomenon demonstrates that the carrageenan network was damaged under large deformation. The compressive strength at 90% strain of the PDN gel largely decayed from 6.08 MPa for the first loading to 5.48, 5.21, 4.95, and 4.72 for the second, third, fourth, and fifth runs. To further investigate the internal fracture and fluid pressurization behavior of PDN hydrogels, the swelling measurements of compression-tested hydrogels were presented in Figure 4e. These PDN hydrogels showed an increasing swelling ratio over time until equilibrium. In PDN hydrogels, the damaged carrageenan fragments linked with PAAm chains afford a robust osmotic pressure to swell the compression-tested PDN hydrogels, leading to an increased swelling ratio from 179.4 ± 17.9% to 253.9 ± 2.5% after one compression cycle and 284.1 ± 12.3% after two compression cycles at equilibrium state. This result indicates that the brittle carrageenan network is damaged under compression, which is consistent with the mechanical results. Cyclic loading-unloading measurements were also performed at predefined strains (30% and 60%) of DN and PDN hydrogels ( Figure S2 and Table S3). With increasing cycles, the compressive strength and hysteresis energy at these predefined maximum strains all depicted a slight decay. Figure 4f presents the representative fifth cycle loop with different maximum strains (30%, 60%, and 90%) of PDN gels. The loading curves with increasing strain overlapped with the previous unloading curves. This observation indicates that PDN hydrogels possess poor fatigue resistance that is similar to other DN hydrogels [43,44]. Therefore, developing an efficient method to fully or partially recover the mechanical property of tough DN hydrogels after compression, is urgent.

Thermal Recovery
To determine the optimized temperature of the gel-sol transition for the DN gels, DSC was used. Figure S3 shows the DSC curve of carrageenan, indicating that the sol-gel transition temperature of carrageenan was around 60 • C. Both compressed GDN and PDN hydrogels were heated around 60 • C to investigate their thermal recovery ability ( Figure 5). After the first compression, the compressive strength and toughness of PDN hydrogels largely decreased due to the breakage of the carrageenan network. The PDN hydrogels could only present 79.00% and 60.38% of the compressive strength and toughness on the second compression, respectively. After thermal treatment, most of the compressive strength (94.88%) and toughness (80.85%) were recovered. Figure 5b shows a similar trend of GDN hydrogels after thermal treatment. These results indicate that the thermal treatment can effectively recover the mechanical properties of GDN and PDN hydrogels by rebuilding a brittle carrageenan network. Although both GDN and PDN presented excellent recovered mechanical properties after thermal treatment, the thermal treatment involved direct heating, making it difficult to be implemented within the human body. In this case, developing a photothermal method to recover the mechanical properties of DN hydrogels is necessarily required. hydrogels after thermal treatment. These results indicate that the thermal treatment can effectively recover the mechanical properties of GDN and PDN hydrogels by rebuilding a brittle carrageenan network. Although both GDN and PDN presented excellent recovered mechanical properties after thermal treatment, the thermal treatment involved direct heating, making it difficult to be implemented within the human body. In this case, developing a photothermal method to recover the mechanical properties of DN hydrogels is necessarily required.

Photothermal Recovery
The PGO was added as reinforcing nanofiller and photothermal agent to improve the mechanical properties of hydrogels and gift the function of precise, specific, and human-friendly heating. As the heating only occurred on the hydrogels, limited damage could happen to the surrounding tissue, which is very suitable for clinic application. The NIR photothermal effect of PGO in PDN hydrogels was systematically investigated. Figure 6a shows the temperature change of DN, GDN, and PDN hydrogels with the 808 nm laser irradiation (2.5 W cm −2 ) for 450 s. No big temperature change was found in DN gels because of a lack of photothermal materials. The temperature of GDN increased from room temperature to ~45 °C within 200 s. However, after the temperature reached 45 °C, no temperature increase was found even by extending irradiation time. The low temperature could not afford enough heat energy for the gel-sol transition of carrageenan to rebuild the damaged network. However, the temperature of PDN could increase to around 60 °C after 360 s under laser exposure, which demonstrates that PGO can efficiently absorb and convert NIR light into thermal energy. These results also indicate that the PDN hydrogels may have the ability to recover its mechanical properties by NIR laser irradiation.

Photothermal Recovery
The PGO was added as reinforcing nanofiller and photothermal agent to improve the mechanical properties of hydrogels and gift the function of precise, specific, and human-friendly heating. As the heating only occurred on the hydrogels, limited damage could happen to the surrounding tissue, which is very suitable for clinic application. The NIR photothermal effect of PGO in PDN hydrogels was systematically investigated. Figure 6a shows the temperature change of DN, GDN, and PDN hydrogels with the 808 nm laser irradiation (2.5 W cm −2 ) for 450 s. No big temperature change was found in DN gels because of a lack of photothermal materials. The temperature of GDN increased from room temperature to~45 • C within 200 s. However, after the temperature reached 45 • C, no temperature increase was found even by extending irradiation time. The low temperature could not afford enough heat energy for the gel-sol transition of carrageenan to rebuild the damaged network. However, the temperature of PDN could increase to around 60 • C after 360 s under laser exposure, which demonstrates that PGO can efficiently absorb and convert NIR light into thermal energy. These results also indicate that the PDN hydrogels may have the ability to recover its mechanical properties by NIR laser irradiation.
The penetration depth was also studied in DN and PDN gels. Figure 6b presents the average temperature changes of the cylindrical DN and PDN gels' top surface under an 808 nm laser with a power density of 2.5 W cm −2 for 10 min. The temperature of DN gels was barely changed under the NIR laser, which implies that no materials in DN gels have the ability to absorb and convert the NIR light to heat. Whereas, the temperature of PDN gels was remarkably enhanced in the first 180 s, which indicates that the PGO possesses the ability to absorb the NIR light and transfer light energy into thermal energy to increase the temperature of PDN gels. In the following 180 s, the temperature of PDN gels was almost steady, which illustrates that the gel-sol transition in PDN gels absorbs a large amount of thermal energy. After that, the temperature of PDN gels was continuously increased in the following 240 s, which showed that the gel-sol transition of PDN gels was completed. Finally, the temperature flattened again. This is because large amounts of heat energy are transferred to the surroundings due to thermal convection, conduction, and irradiation. As shown in Figure 6c, the temperature distribution on the top surface of cylindrical PDN gels was presented, and the temperature on the edge of PDN gels was lower than the temperature in the center of PDN gels. Therefore, the PGO effectively converts the NIR light into thermal energy to rebuild the carrageenan network, which may partially or fully recover the mechanical property of PDN gels. The penetration depth was also studied in DN and PDN gels. Figure 6b presents the average temperature changes of the cylindrical DN and PDN gels' top surface under an 808 nm laser with a power density of 2.5 W cm −2 for 10 min. The temperature of DN gels was barely changed under the NIR laser, which implies that no materials in DN gels have the ability to absorb and convert the NIR light to heat. Whereas, the temperature of PDN gels was remarkably enhanced in the first 180 s, which indicates that the PGO possesses the ability to absorb the NIR light and transfer light energy into thermal energy to increase the temperature of PDN gels. In the following 180 s, the temperature of PDN gels was almost steady, which illustrates that the gel-sol transition in PDN gels absorbs a large amount of thermal energy. After that, the temperature of PDN gels was continuously increased in the following 240 s, which showed that the gel-sol transition of PDN gels was completed. Finally, the temperature flattened again. This is because large amounts of heat energy are transferred to the surroundings due to thermal convection, conduction, and irradiation. As shown in Figure 6c, the temperature distribution on the top surface of cylindrical PDN gels was presented, and the temperature on the edge of PDN gels was lower than the temperature in the center of PDN gels. Therefore, the PGO effectively converts the NIR light into thermal energy to rebuild the carrageenan network, which may partially or fully recover the mechanical property of PDN gels. Figure 7a presents the compressive stress-strain curves of PDN gels at the first compression, second compression, and second compression with NIR laser irradiation. After NIR laser irradiation, the compressive strength and toughness were clearly increased (the insert in Figure 7a) from 73.40% to 89.90%, and from 53.91% to 66.50%, respectively. The recovery efficiency of NIR laser treatment is a little lower than that of the thermal treatment. The reason may be that the edge of the PDN Figure 6. (a) Temperature change of DN, GDN, and PDN hydrogels with NIR laser irradiation (808 nm, 2.5 W cm −2 ) detected by a thermometer, (b) fitted curve of average temperature of DN and PDN hydrogels with NIR laser irradiation (808 nm, 2.5 W cm −2 ) for 10 min detected by a high-precision digital thermometer (Omega), and (c) infrared images of temperature distribution in DN and PDN gels with NIR laser irradiation (808 nm, 2.5 W cm −2 ) for 600 s. Figure 7a presents the compressive stress-strain curves of PDN gels at the first compression, second compression, and second compression with NIR laser irradiation. After NIR laser irradiation, the compressive strength and toughness were clearly increased (the insert in Figure 7a) from 73.40% to 89.90%, and from 53.91% to 66.50%, respectively. The recovery efficiency of NIR laser treatment is a little lower than that of the thermal treatment. The reason may be that the edge of the PDN hydrogels had a temperature below 60 • C due to thermal release, leading to unfinished thermal recovery. These results demonstrate that the PDN gels have the ability to partially recover their mechanical properties by NIR laser irradiation. Figure 7b presents a schematic diagram of the NIR photothermal recovery process. The PGO nanosheets work as heating sources by absorbing and converting light energy into thermal energy. The broken carrageenan network is rebuilt by absorbing thermal energy deriving from PGO. After cooling, the mechanical properties of PDN are recovered. hydrogels had a temperature below 60 °C due to thermal release, leading to unfinished thermal recovery. These results demonstrate that the PDN gels have the ability to partially recover their mechanical properties by NIR laser irradiation. Figure 7b presents a schematic diagram of the NIR photothermal recovery process. The PGO nanosheets work as heating sources by absorbing and converting light energy into thermal energy. The broken carrageenan network is rebuilt by absorbing thermal energy deriving from PGO. After cooling, the mechanical properties of PDN are recovered. Figure 8a-c present microscopic images of the healed surface of carrageenan gels after thermal treatment, which demonstrate that carrageenan gels can be reformed by thermal-melting. Figure 8e depicts the surface of the PDN hydrogels with a crack by a knife. After 808 nm laser irradiation, the crack was healed with a small scar on the PDN gels' surface. These results demonstrate that the PDN hydrogels can be healed by NIR laser irradiation due to the presence of PGO. Therefore, these photothermal reversible and tough hydrogels were successfully developed, which may be employed as promising substitutes in load-bearing tissues.

Conclusions
In summary, biocompatible porphyrin decorated graphene oxide (PGO) reinforced carrageenan/polyacrylamide double network hydrogels (PDN) were first developed. With the introduction of PGO, the compressive strength of PDN was improved at 104% compared to that of carrageenan/polyacrylamide double network hydrogels (DN). In addition, the fatigue resistance of Figure 8a-c present microscopic images of the healed surface of carrageenan gels after thermal treatment, which demonstrate that carrageenan gels can be reformed by thermal-melting. Figure 8e depicts the surface of the PDN hydrogels with a crack by a knife. After 808 nm laser irradiation, the crack was healed with a small scar on the PDN gels' surface. These results demonstrate that the PDN hydrogels can be healed by NIR laser irradiation due to the presence of PGO. Therefore, these photothermal reversible and tough hydrogels were successfully developed, which may be employed as promising substitutes in load-bearing tissues. hydrogels had a temperature below 60 °C due to thermal release, leading to unfinished thermal recovery. These results demonstrate that the PDN gels have the ability to partially recover their mechanical properties by NIR laser irradiation. Figure 7b presents a schematic diagram of the NIR photothermal recovery process. The PGO nanosheets work as heating sources by absorbing and converting light energy into thermal energy. The broken carrageenan network is rebuilt by absorbing thermal energy deriving from PGO. After cooling, the mechanical properties of PDN are recovered. Figure 8a-c present microscopic images of the healed surface of carrageenan gels after thermal treatment, which demonstrate that carrageenan gels can be reformed by thermal-melting. Figure 8e depicts the surface of the PDN hydrogels with a crack by a knife. After 808 nm laser irradiation, the crack was healed with a small scar on the PDN gels' surface. These results demonstrate that the PDN hydrogels can be healed by NIR laser irradiation due to the presence of PGO. Therefore, these photothermal reversible and tough hydrogels were successfully developed, which may be employed as promising substitutes in load-bearing tissues.

Conclusions
In summary, biocompatible porphyrin decorated graphene oxide (PGO) reinforced carrageenan/polyacrylamide double network hydrogels (PDN) were first developed. With the introduction of PGO, the compressive strength of PDN was improved at 104% compared to that of carrageenan/polyacrylamide double network hydrogels (DN). In addition, the fatigue resistance of

Conclusions
In summary, biocompatible porphyrin decorated graphene oxide (PGO) reinforced carrageenan/polyacrylamide double network hydrogels (PDN) were first developed. With the introduction of PGO, the compressive strength of PDN was improved at 104% compared to that of carrageenan/polyacrylamide double network hydrogels (DN). In addition, the fatigue resistance of PDN was systematically investigated, which indicated that the PDN possesses poor fatigue resistance similar to other previous double network hydrogels. After near-infrared (NIR) laser irradiation, around 90% compressive strength was recovered, which demonstrates that the PGO efficiently absorb and convert NIR light energy into thermal energy and the carrageenan network is rebuilt during the photothermal and cooling period. Therefore, by introducing PGO into DN hydrogels, the PDN hydrogels possess photothermal recovery and superior mechanical properties, which may find applications as promising substitutes for load-bearing tissues.
Supplementary Materials: The following are available online at http://www.mdpi.com/2079-4991/9/10/1487/s1, Figure S1: Zeta potential of (a) GO and (b) PGO, Figure S2: Representative cyclic loading−unloading curves of (a) DN and (b) PDN hydrogels for up to five cycles at 30% and 60% strain compression, Figure S3: DSC curves of carrageen, Table S1: Compressive properties of tough hydrogels with a maximum strain at 99%, Table S2: Compressive Properties of tough hydrogels with different strain, Table S3: Cyclic compressive properties of tough hydrogel with different strain.