Iodoxybenzoic Acid Supported on Multi Walled Carbon Nanotubes as Biomimetic Environmental Friendly Oxidative Systems for the Oxidation of Alcohols to Aldehydes

Iodoxybenzoic acid (IBX) supported multi walled carbon nanotube (MWCNT) derivatives have been prepared as easily recyclable solid reagents. These compounds have been shown to be able to mimic the alcohol dehydrogenases and monooxygenases promoted oxidation of aromatic alcohols to corresponding aldehydes. Their reactivity was found to be dependent on the degree of functionalization of MWCNTs as well as from the chemical properties of the spacers used to bind IBX on the surface of the support. Au-decorated MWCNTs and the presence of longer spacers resulted in the optimal experimental conditions. A high conversion of the substrates and yield of desired products were obtained.


Introduction
The oxidation of alcohols to corresponding carbonyl compounds is one of the most fundamental and important processes in synthetic organic chemistry. Although a variety of methods and reagents have been developed, they all suffer from the difficulty of selectively oxidizing primary alcohols to aldehydes without the concomitant formation of carboxylic acids and other over-oxidation products [1]. The oxidation of alcohols to aldehydes is usually performed in the presence of stoichiometric reagents [2] including the Dess-Martin oxidation [3], the Swern and Corey-Kim reaction [4], and the Burgess reagent [5]. Heavy metal reagents have been also used in catalytic procedures, for instance, hydrogen-transfer reactions (Ru, Rh, Ir) [6], and Oppenauer oxidations (Al, Zr, lanthanides) [4]. On the other hand, metal-free oxidations are desired processes in the context of green-chemistry due to the known toxicity and high environmental impact of metal species. In this context, biotechnological applications of oxidative enzymes, e.g., alcohol dehydrogenases and monooxygenases with high vacuum PHI 1257 system and an Agilent 7500 ICP-MS instrument under clean room ISO6 (Santa Clara, CA, USA), respectively.

Preparation of oxMWCNTs I
In a round-bottomed flask, equipped with an egg-shaped magnetic stirring bar, MWCNTs and a mixture of concentrated H 2 SO 4 -HNO 3 (3:1) were stirred for 4.0 h at r.t. and an additional 12 h at 40 • C. The reaction mixture was cooled down to r.t. and cold H 2 O (400 mL) was poured into the reactor. The mixture was washed by centrifugation at 4000× g rpm (30 min), and the supernatant was removed. The remaining solid was further washed with deionized H 2 O (200 mL). At each washing step, the mixture was centrifuged (4000 rpm for 30 min), filtered using GH Polypro membrane filters 0.2 µm and the supernatant was removed. The resulting oxidized MWCNTs (oxMWCNTs I) were dried in vacuo and used without further purification.

Preparation of Oxidizing Solid Reagents VIII A-B
MWCNTs (100 mg) were sonicated in 100 mL of ethanol for 2 h. Afterwards, 8.5 mL of 0.1 M HAuCl 4 ethanolic solution was added. In order to obtain Au particles, reduction with 300 mg of NaBH 4 was carried out by stirring for about 30 min. Then, Au-MWCNTs V was isolated by centrifugation and filtered using GH Polypro membrane filters 0.2 µm washed several times with ethanol and dried at 80 • C. 2-amino-1-ethanethiol (for NH 2 -Au-MWCNTs VI A) and 6-amino-1-hexanthiol (for NH 2 -Au-MWCNTs VI B) was dissolved in a mixture of water (20 mL) and 1.0 M HCl (3.0 mL). Au-MWCNTs V (30 mg) and ethanol (3.0 mL) were added and the mixture was left under magnetic stirring for 24 h. After that time, the product was isolated by centrifugation, washed three times with 0.01 M NaOH and ethanol, and filtered using GH Polypro membrane filters 0.2 mm. Resulting NH 2 -Au-MWCNTs VI A-B were dried under argon stream. NH 2 -Au-MWCNTs VI A-B (200 mg) were suspended in DMF (0.8 mg/mL) and treated with DIC (790 mg, 6 mmol) and DIPEA (2.1 mL, 12 mmol) in a 500 mL round-bottomed flask with an egg-shaped magnetic stirring bar. Thereafter, IBA (1.5 g, 6 mmol) was added to the solution, and the mixture was stirred for 8 h at 30 • C. The resulting IBA-Au-MWCNTs VII A-B were washed with DMF and H 2 O by centrifugation (4000× g rpm, 20 min) and filtered using GH Polypro membrane filters 0.2 µm. IBA-Au-MWCNTs VII A-B were suspended in H 2 O (125 mg/250 mL) in a round-bottomed flask, then Oxone ® (950 mg, 1.5 mmol), and methane sulfonic acid (100 µL, 1.5 mmol) were added and stirred for 8 h at r.t. Thereafter, IBX-Au-MWCNTs VIII A-B were washed with DMF (5 × 10 mL) and H 2 O (3 × 10 mL) and filtered using GH Polypro membrane filters 0.2 µm.

Preparation of Oxidizing Solid Reagent VIII-C
11-mercapto-1-undecanol was dissolved in a mixture of water (20 mL) and 1.0 M HCl (3.0 mL). Au-MWCNTs V (30 mg) and ethanol (3.0 mL) were added and the mixture was left under magnetic stirring for 24 h. After that time, the product was isolated by centrifugation, washed three times with 0.01 M NaOH and ethanol, and filtered using GH Polypro membrane filters 0.2 mm. The resulting OH-Au-MWCNTs VI C was dried under argon stream. OH-Au-MWCNTs VI C (200 mg) was suspended in DMF (0.8 mg/mL) and treated with DIC (790 mg, 6 mmol), and DIPEA (2.1 mL, 12 mmol) in a 500 mL round-bottomed flask with an egg-shaped magnetic stirring bar. Thereafter, IBA (1.5 g, 6.0 mmol) was added to the solution and the mixture stirred for 8 h at 30 • C. The resulting IBA-Au-MWCNTs VII C was washed with DMF and H 2 O by centrifugation (4000× g rpm, 20 min) and filtered using GH Polypro membrane filters 0.2 µm. IBA-Au-MWCNTs VII C was suspended in H 2 O (125 mg/250 mL) in a round-bottomed flask, then Oxone ® (950 mg, 1.5 mmol) and methane sulfonic acid (100 µL, 1.5 mmol) were added and stirred for 8 h at r.t. Thereafter, IBX-Au-MWCNTs VIII C was washed with DMF (5 × 10 mL) and H 2 O (3 × 10 mL) and filtered using GH Polypro membrane filters 0.2 µm.

Transmission Electron Microscopy (TEM), Scanning Electron Microscopy (SEM), and X-Ray Photoelectron Spectroscopy (XPS) Analyses
For transmission electron microscopy (TEM), samples were suspended in bi-distilled water. Droplets of sample suspensions (10 µL) were placed on formvar-carbon coated grids and allowed to adsorb for 60 s. Excess liquid was removed gently by touching the filter paper. Samples were observed with a JEOL 1200 EX II electron microscope (Waltham, MA, USA). Micrographs were acquired by the Olympus SIS VELETA CCD camera equipped with iTEM software (Waltham, MA, USA). For scanning electron microscopy (SEM), the sample suspensions (50 µL) were let to adsorb onto carbon tape attached to aluminum stubs and air dried at 25 • C. The observation was made by a JEOL JSM 6010LA electron microscope (Waltham, MA, USA) using Scanning Electron (SE) and Back Scattered Electrons (BSE) detectors. Energy Dispersive Spectroscopy (EDS) analysis was carried out to reveal the chemical elements. X-ray photoelectron spectroscopy (XPS) analysis was done in an ultrahigh vacuum PHI 1257 system equipped with a hemispherical analyzer, operating in the constant pass energy mode (with the total energy resolution of 0.8 eV) and using a non-monochromatized Mg Kα radiation source. The distance between the sample and the anode was about 40 mm, the illumination area was about 1 × 1 cm 2 , and the analyzed area was 0.8 × 2.0 mm 2 with a take-off angle between the sample surface and the photoelectron energy analyzer of 45 • . The energy scale was calibrated with reference to the binding energy of the C 1s at 284.8 eV with respect to the Fermi level. Survey scans of the III-B, IV-B, VII-A, and VIII-A compounds acquired in the range of 0-1100 eV (not shown here) displayed the contribution coming from the main elements involved in the reaction process for all of the samples: carbon, nitrogen, oxygen, sulfur, gold, and iodine. No contaminant species were observed within the sensitivity of the technique.

Inductively Coupled Plasma Mass-Spectrometry (ICP-MS) Analysis
The samples were weighed (from 1.6 to 6.9 mg) and transferred in Fluorinated ethylene propylene (FEP) vials, previously washed to avoid any kind of external contamination. Regia solution was chosen for the mineralization as it combines the oxidizing capacities of HNO 3 with the complexing capacities of chlorides against I 2 produced during digestion. In particular, 750 µL of HCl and 150 µL HNO 3 were added and the solution was heated to 80 • C for 3 hours. The volume was adjusted to 5.0 mL and then diluted another 10 times before the ICP-MS analysis. The analysis was performed with an Agilent 7500 ICP-MS instrument (Palo Alto, CA, USA). Four standards at 10, 20, 50, and 100 ppb of iodine and gold were used for calibrating the instrument.

Oxidation of Aromatic Alcohols
The oxidation of alcohols 1-8 (1.0 mmol) in EtOAc (10 mL) was performed by adding the appropriate solid reagent (IV A-B or VIII A-C, 1.2 eq) to a single neck round-bottomed flask equipped with a water condenser under magnetic stirring at reflux conditions (c.a. 80 • C) for 24 h. At the end of the oxidation, IV A-B and VIII A-C were filtered off using GH Polypro membrane filters 0.2 µm and washed with EtOAc (5 × 10 mL). The yield of aldehydes 9-16 was determined by GC-MS analysis using n-dodecane (0.1 mmol) as an internal standard. The reactions were performed in triplicate. GC-MS was performed using a VF-5ms column (30 m, 0.25 mm, 0.25 µm) through the following program: injection temperature 280 • C, detector temperature 280 • C, gradient 50 • C for 2 min, and 10 • C/min for 60 min, flow velocity of the carrier (helium), 1.0 mL min −1 . In order to identify the structures of the products, two strategies were followed. First, the spectra of identifiable peaks were compared with commercially available electron mass spectrum libraries such as that of National Institute of Standards and Technology (NIST-Fison, Manchester, UK). In this latter case, spectra with at least 98% similarity were chosen. Secondly, GC-MS analysis was repeated using commercially available standard compounds. The original mass spectra of compounds 9-16 are reported in Figure  S1 (Supporting Information).

Preparation of IBX Supported MWCNTs and MWCNTs-Au Oxidizing Solid Reagents
The immobilization of IBX on MWCNTs was first based on the formation of an amide-type linkage between the spacer functionalized MWCNTs and 2-iodobenzoic acid (IBA), followed by activation of IBA to IBX (Scheme 1). In particular, commercially available MWCNTs were oxidized with HNO 3 /H 2 SO 4 to oxMWCNTs I with the aim of increasing the amount of polar moieties (alcoholic and acidic groups) on the surface [23]. Next, ox-MWCNTs I was functionalized with selected alkyl diamino spacers (1,2-di-aminoethane and 1,6-diaminoethane) by coupling with N,N-diisopropyl carbodiimide (DIC) and 1-hydroxy benzotriazole (HOBt) in DMF at room temperature for 24 hours to yield the intermediates II A-B. The effectiveness of the coupling procedure was confirmed by Fourier Transform Infrared Spectroscopy (FTIR) analysis for II-A as a selected example. In particular, the peak at 1649 cm −1 , corresponding to the stretching vibration of the carboxylic groups in oxMWCNTs I ( Figure S2), was shifted to 1633 cm −1 in II-A as a consequence of the amide formation, in accordance with data previously reported for the functionalization of MWCNTs ( Figure S3) [24]. The intermediates II A-B were successively suspended in DMF and treated with IBA at room temperature for 24 hours in the presence of DIC and HOBt to afford IBA-MWCNTs III A-B. The formation of the novel amide linkage was again confirmed by the shift of the amide peak from 1633 cm −1 to 1627 cm −1 ( Figure S4). Finally, III A-B were activated to IBX-MWCNTs IV A-B by reaction with Oxone ® and methansulfonic acid. In this latter case, only a slight shift of the amide peak toward 1606 cm −1 was observed ( Figure S5) [20].

Oxidation of Aromatic Alcohols
The oxidation of alcohols 1-8 (1.0 mmol) in EtOAc (10 mL) was performed by adding the appropriate solid reagent (IV A-B or VIII A-C, 1.2 eq) to a single neck round-bottomed flask equipped with a water condenser under magnetic stirring at reflux conditions (c.a. 80° C) for 24 h. At the end of the oxidation, IV A-B and VIII A-C were filtered off using GH Polypro membrane filters 0.2 μm and washed with EtOAc (5 × 10 mL). The yield of aldehydes 9-16 was determined by GC-MS analysis using n-dodecane (0.1 mmol) as an internal standard. The reactions were performed in triplicate. GC-MS was performed using a VF-5ms column (30 m, 0.25 mm, 0.25 µ m) through the following program: injection temperature 280 °C, detector temperature 280 °C, gradient 50 °C for 2 min, and 10 °C/min for 60 min, flow velocity of the carrier (helium), 1.0 mL min −1 . In order to identify the structures of the products, two strategies were followed. First, the spectra of identifiable peaks were compared with commercially available electron mass spectrum libraries such as that of National Institute of Standards and Technology (NIST-Fison, Manchester, UK). In this latter case, spectra with at least 98% similarity were chosen. Secondly, GC-MS analysis was repeated using commercially available standard compounds. The original mass spectra of compounds 9-16 are reported in Figure S1 (Supporting Information).

Preparation of IBX Supported MWCNTs and MWCNTs-Au Oxidizing Solid Reagents
The immobilization of IBX on MWCNTs was first based on the formation of an amide-type linkage between the spacer functionalized MWCNTs and 2-iodobenzoic acid (IBA), followed by activation of IBA to IBX (Scheme 1). In particular, commercially available MWCNTs were oxidized with HNO3/H2SO4 to oxMWCNTs I with the aim of increasing the amount of polar moieties (alcoholic and acidic groups) on the surface [23]. Next, ox-MWCNTs I was functionalized with selected alkyl diamino spacers (1,2-di-aminoethane and 1,6-diaminoethane) by coupling with N,N-diisopropyl carbodiimide (DIC) and 1-hydroxy benzotriazole (HOBt) in DMF at room temperature for 24 hours to yield the intermediates II A-B. The effectiveness of the coupling procedure was confirmed by Fourier Transform Infrared Spectroscopy (FTIR) analysis for II-A as a selected example. In particular, the peak at 1649 cm −1 , corresponding to the stretching vibration of the carboxylic groups in oxMWCNTs I ( Figure S2), was shifted to 1633 cm −1 in II-A as a consequence of the amide formation, in accordance with data previously reported for the functionalization of MWCNTs ( Figure S3) [24]. The intermediates II A-B were successively suspended in DMF and treated with IBA at room temperature for 24 hours in the presence of DIC and HOBt to afford IBA-MWCNTs III A-B. The formation of the novel amide linkage was again confirmed by the shift of the amide peak from 1633 cm −1 to 1627 cm −1 ( Figure S4). Finally, III A-B were activated to IBX-MWCNTs IV A-B by reaction with Oxone ® and methansulfonic acid. In this latter case, only a slight shift of the amide peak toward 1606 cm −1 was observed ( Figure S5) [20].

Scheme 1. Preparation of IBX supported MWCNTs oxidizing solid reagents IV A-B.
As an alternative, Au decorated Au-MWCNTs V were used instead of oxMWCNTs I as anchorage supports. Briefly, Au-MWCNTs V [25] were treated with selected alkyl mercapto-amino spacers (2-amino-1-ethanethiol and 6-amino-1-hexanthiol, respectively) in an acidic water/ethanol mixture (pH 2, HCl 1.0 M) to afford the intermediates NH2-Au-MWCNTs VI A-B by formation of As an alternative, Au decorated Au-MWCNTs V were used instead of oxMWCNTs I as anchorage supports. Briefly, Au-MWCNTs V [25] were treated with selected alkyl mercapto-amino spacers (2-amino-1-ethanethiol and 6-amino-1-hexanthiol, respectively) in an acidic water/ethanol mixture (pH 2, HCl 1.0 M) to afford the intermediates NH 2 -Au-MWCNTs VI A-B by formation of covalent Au-sulfur bonds (Scheme 2). These intermediates were successively suspended in DMF and treated with IBA at room temperature for 24 h in the presence of DIC and HOBt to yield IBA-Au-MWCNTs VII A-B. Finally, IBX-Au-MWCNTs VIII A-B were obtained through the reaction of VII A-B with Oxone ® and methansulfonic acid [20]. The TEM images of IV B and VIII B, as the selected samples, are reported in Figure 1 (Panel A and C). In VIII B, the black-spots represent the Au particles, whose presence was unambiguously confirmed by SEM associated to BSE analysis ( Figure S6). Note that the structural integrity of the MWCNTs was retained after the loading of IBX. covalent Au-sulfur bonds (Scheme 2). These intermediates were successively suspended in DMF and treated with IBA at room temperature for 24 h in the presence of DIC and HOBt to yield IBA-Au-MWCNTs VII A-B. Finally, IBX-Au-MWCNTs VIII A-B were obtained through the reaction of VII A-B with Oxone ® and methansulfonic acid [20]. The TEM images of IV B and VIII B, as the selected samples, are reported in Figure 1 (Panel A and C). In VIII B, the black-spots represent the Au particles, whose presence was unambiguously confirmed by SEM associated to BSE analysis ( Figure  S6). Note that the structural integrity of the MWCNTs was retained after the loading of IBX.  Moreover, VIII C was prepared using a longer thio-alcohol spacer (11-mercapto-1-undecanol), with the aim to bind IBA through the formation of an ester bond instead of an amide bond (Scheme 3). Briefly, Au-MWCNTs V was treated with 11-mercapto-1-undecanol in HCl 1.0 M and EtOH to afford the intermediates VI C by formation of covalent Au-sulfur bonds (Scheme 3). This intermediate was successively treated with DIC, DIPEA, and IBA to yield VII C. Finally, VII C was suspended in H2O and treated with Oxone ® and methansulfonic acid to afford VIII C.  [20]. The TEM images of IV B and VIII B, as the selected samples, are reported in Figure 1 (Panel A and C). In VIII B, the black-spots represent the Au particles, whose presence was unambiguously confirmed by SEM associated to BSE analysis ( Figure  S6). Note that the structural integrity of the MWCNTs was retained after the loading of IBX.  Moreover, VIII C was prepared using a longer thio-alcohol spacer (11-mercapto-1-undecanol), with the aim to bind IBA through the formation of an ester bond instead of an amide bond (Scheme 3). Briefly, Au-MWCNTs V was treated with 11-mercapto-1-undecanol in HCl 1.0 M and EtOH to afford the intermediates VI C by formation of covalent Au-sulfur bonds (Scheme 3). This intermediate was successively treated with DIC, DIPEA, and IBA to yield VII C. Finally, VII C was suspended in H2O and treated with Oxone ® and methansulfonic acid to afford VIII C. Moreover, VIII C was prepared using a longer thio-alcohol spacer (11-mercapto-1-undecanol), with the aim to bind IBA through the formation of an ester bond instead of an amide bond (Scheme 3). Briefly, Au-MWCNTs V was treated with 11-mercapto-1-undecanol in HCl 1.0 M and EtOH to afford the intermediates VI C by formation of covalent Au-sulfur bonds (Scheme 3). This intermediate was successively treated with DIC, DIPEA, and IBA to yield VII C. Finally, VII C was suspended in H 2 O and treated with Oxone ® and methansulfonic acid to afford VIII C.  Figure 2 presents the detailed spectra of the C 1s, O 1s, N 1s, S 2p, Au 4f, and I 3d peaks of III B, IV B, VII A, and VIII A. All spectra were normalized to C 1s, which corresponded to the signal due to the MWCNTs support. In this way, we have the possibility of comparing the different peaks. XPS analysis clearly confirmed the presence of iodine and gold in the analyzed samples. Therefore, from the intensity of the XPS peaks ( Figure 2) after the last step of the sample preparation (III B → IV B and VII A → VIII A), a slight leaching of Au and I was observed. The C 1s spectra were fitted by the sum of five components assigned to C atoms belonging to: aromatic rings carbon (C=C/C-C, 284.8 eV), hydroxyl groups (C-OH, 285.9 eV), epoxy groups (C-O-C, 286.9 eV), carbonyl groups (C=O, 288.2 eV), and carboxyl groups (C=O(OH), 289.3 eV) (the hump at 290.6 eV was assigned to a π-π* shake-up satellite (in line with [20]). The O 1s spectra were fitted by the sum of three components: OH-C (533.4 eV), C-O-C (532 eV), and O=C (530.4 eV) [26]. Electron binding energies of the peak positions of N 1s, S 2p3/2, Au 4f7/2, and I 3d5/2 for all samples are listed in Table 1.  Figure 2 presents the detailed spectra of the C 1s, O 1s, N 1s, S 2p, Au 4f, and I 3d peaks of III B, IV B, VII A, and VIII A. All spectra were normalized to C 1s, which corresponded to the signal due to the MWCNTs support. In this way, we have the possibility of comparing the different peaks. XPS analysis clearly confirmed the presence of iodine and gold in the analyzed samples. Therefore, from the intensity of the XPS peaks ( Figure 2) after the last step of the sample preparation (III B → IV B and VII A → VIII A), a slight leaching of Au and I was observed.  Figure 2 presents the detailed spectra of the C 1s, O 1s, N 1s, S 2p, Au 4f, and I 3d peaks of III B, IV B, VII A, and VIII A. All spectra were normalized to C 1s, which corresponded to the signal due to the MWCNTs support. In this way, we have the possibility of comparing the different peaks. XPS analysis clearly confirmed the presence of iodine and gold in the analyzed samples. Therefore, from the intensity of the XPS peaks ( Figure 2) after the last step of the sample preparation (III B → IV B and VII A → VIII A), a slight leaching of Au and I was observed. The C 1s spectra were fitted by the sum of five components assigned to C atoms belonging to: aromatic rings carbon (C=C/C-C, 284.8 eV), hydroxyl groups (C-OH, 285.9 eV), epoxy groups (C-O-C, 286.9 eV), carbonyl groups (C=O, 288.2 eV), and carboxyl groups (C=O(OH), 289.3 eV) (the hump at 290.6 eV was assigned to a π-π* shake-up satellite (in line with [20]). The O 1s spectra were fitted by the sum of three components: OH-C (533.4 eV), C-O-C (532 eV), and O=C (530.4 eV) [26]. Electron binding energies of the peak positions of N 1s, S 2p3/2, Au 4f7/2, and I 3d5/2 for all samples are listed in The C 1s spectra were fitted by the sum of five components assigned to C atoms belonging to: aromatic rings carbon (C=C/C-C, 284.8 eV), hydroxyl groups (C-OH, 285.9 eV), epoxy groups (C-O-C, 286.9 eV), carbonyl groups (C=O, 288.2 eV), and carboxyl groups (C=O(OH), 289.3 eV) (the hump at 290.6 eV was assigned to a π-π* shake-up satellite (in line with [20]). The O 1s spectra were fitted by the sum of three components: OH-C (533.4 eV), C-O-C (532 eV), and O=C (530.4 eV) [26]. Electron binding energies of the peak positions of N 1s, S 2p 3/2 , Au 4f 7/2 , and I 3d 5/2 for all samples are listed in Table 1.

Determination of the Iodine Loading Factor by ICP-MS Analysis
The iodine Loading Factor (LF) for IV A-B and VIII A-C, defined as mmol of iodine per gram of support, was measured by Inductively Coupled Plasma Mass-Spectrometry (ICP-MS) analysis ( Table 2). As reported in Table 2, IV B showed a Loading Factor (LF) significantly higher than IV A (entry 2 versus entry 1), highlighting the easier immobilization of IBA in the presence of the longer spacer (that is 1,6-diaminoethane versus 1,2-diaminoethane) [27]. VIII A and VIII B showed LF values of 0.4 and 0.7, respectively, while for VIII C, the iodine LF was found to be 0.3 (Table 1, entries 3-5).
The LF values found for IV A-B and VIII A-C were of the same order of magnitude, and higher than those previously reported for solid reagents based on the immobilization of IBX on both polymer resins and GO [14,20,25]. Moreover, the higher amount of Au with respect to iodine measured for VIII A-C proved that the initial linkage of mercapto containing spacers was not quantitative with respect to the Au binding sites available on the support ( Table 2, entries 3-5).

Oxidation of Aromatic Alcohols with IV A-B and VIII A-C
The mechanism of the oxidation of aromatic alcohols with IBX is reported in Scheme 4. The oxygen atom transfer from IBX to the substrate requires the initial addition of the alcohol on activated iodine followed by water elimination and disproportionation with the displacement of the aldehyde [14]. The mechanism of the oxidation of aromatic alcohols with IBX is reported in Scheme 4. The oxygen atom transfer from IBX to the substrate requires the initial addition of the alcohol on activated iodine followed by water elimination and disproportionation with the displacement of the aldehyde [14]. IV A-B and VIII A-C were applied for the oxidation of a large panel of aromatic alcohols, including benzyl alcohols 1-6 and phenethyl alcohols 7,8 (Scheme 5, Tables 4 and 5).

Scheme 5. Oxidation of alcohols 1-8 with IV A-B and VIII A-B.
Homogeneous IBX showed a reactivity higher than the supported reagents in the oxidation of benzyl alcohol 1, probably as a consequence of the diffusional barriers for the access of substrate to active iodine atom, with the only exception of VIII-B, which showed a comparable efficacy (Table 4, entry 1 versus entry 11). On one hand, IV A-B and VIII A-B oxidized benzyl alcohol 1 to aldehyde 9 in a higher yield with respect to sIBX, suggesting the beneficial role of MWCNTs as support with respect to the organic resin (Tables 4 and 5). Irrespective of the experimental conditions, VIII-C was totally ineffective in the oxidation of 1, and was not further investigated (Table 5, entry 19). Probably,

Scheme 5. Oxidation of alcohols 1-8 with IV A-B and VIII A-B.
The reactions were performed treating the appropriate alcohol (1.0 mmol) with a slight excess of IV A-B and VIII A-C (1.2 IBX equivalent calculated on the basis of the specific LF value) in EtOAc (10 mL) at 80 • C for 24 h. Tentatively performing the oxidation in other reaction solvents usually applied for IBX transformations (e.g., Dimethyl Sulfoxide (DMSO) and water) were unsuccessful.
Temperatures lower than c.a. 80 • C were not effective, while at temperatures higher than 80 • C, the reagents showed low stability affording only complex mixtures of reaction products. The reactions were analyzed by gas chromatography mass spectrometry (GC-MS) through a comparison with the original standards. Mass-to-charge ratio (m/z) values of aldehydes 9-16 are reported in Table 3 (the original MS fragmentation spectra are in Figure S1). Under optimal conditions, aromatic aldehydes 9-16 were detected as the only recovered products aside from unreacted substrates (Tables 3 and 4). In the case of the oxidation of benzyl alcohol 1, the reaction with commercially available IBX and with IBX supported on polystyrene (sIBX) were performed as references (Table 3, entries 1 and 2). Table 3. Mass-to-charge ratio (m/z) value and the abundance of mass spectra peaks of compounds 9-16.  Homogeneous IBX showed a reactivity higher than the supported reagents in the oxidation of benzyl alcohol 1, probably as a consequence of the diffusional barriers for the access of substrate to active iodine atom, with the only exception of VIII-B, which showed a comparable efficacy (Table 4, entry 1 versus entry 11). On one hand, IV A-B and VIII A-B oxidized benzyl alcohol 1 to aldehyde 9 in a higher yield with respect to sIBX, suggesting the beneficial role of MWCNTs as support with respect to the organic resin (Tables 4 and 5). Irrespective of the experimental conditions, VIII-C was totally ineffective in the oxidation of 1, and was not further investigated (Table 5, entry 19). Probably, the low reactivity of VIII-C was ascribable to the detrimental effect of the ester linkage with respect to the amide counterpart on the stability of the Iodine (V) active species [28]. As a general trend, benzyl alcohol derivatives 1-6 were more reactive than phenethyl alcohols 7,8. Moreover, benzyl alcohol bearing electron donating substituents 2-5 were more reactive than 1 (Tables 4 and 5), in accordance with previously reported data focusing on the role of the electron density on the benzylic position in the rate-determining step of IBX-mediated oxidations [14].  The dimension of the spacer also played a significant role, where IV-B and VIII-B bearing the longer spacer chains were the most reactive systems. The effect of the spacer on the reactivity of the supported reagents has been previously investigated, the increase of the length of the chains always being related to the increase of the low energy conformational changes attained by the reagent and to the reduction of the diffusional barrier for the substrates [29]. Finally, Au-MWCNTs based reagents VIII A-B were generally more reactive than the MWCNTs counterparts IV A-B, most likely due to the increased electron-transfer properties of the support as a consequence of the increased conductance of nanotubes in the Au carbon junctions [30]. The recyclability of supported IBX was evaluated for the more reactive VIII B reagent in the oxidation of benzylic alcohol 1. After the first run, the reagent was recovered by filtration, washed with EtOAc, dried and restored in the active form by treatment with Oxone ® and methansulfonic acid. VIII B retained the same reactivity to afford aldehyde 9 in a quantitative yield for at least five successive runs. The absence of leaching of IBX from VIII B was confirmed by testing the oxidative capacity of the organic solution recovered after filtration of its EtOAc solution once maintained at reflux under the same experimental conditions applied for the oxidation. Any oxidation capacity was observed.
Compounds IV B and VIII B retained their morphological structural integrity after the oxidation of alcohol 1, as highlighted by the TEM analysis of the recovered samples ( Figure 1, panels B and D, respectively).

Conclusions
The preparation of a series of IBX based reagents supported on MWCNTs, as heterogeneous biomimetic systems for the selective oxidation of primary alcohols to the corresponding aldehydes under mild conditions, has been described. Two different types of carbon structures have been investigated, namely oxidized MWCNTs or an alternative, Au-decorated MWCNTs. The immobilization of the iodine active reagent was realized by exploiting the direct formation of an amide linkage with IBX, mediated by different spacer lengths, or through the high binding affinity of sulfur containing linkers in the case of the Au-decorated MWCNTs. In general, the benzyl alcohol derivatives were shown to be more active than the corresponding phenethyl alcohols, thus confirming the prominent role exerted by the electron density on the benzylic carbon in the rate-determining step of the IBX-mediated oxidative process [29]. In accordance with this hypothesis, benzyl alcohol bearing electron donating substituents showed the highest reactivity. The dimension of the spacer incorporated between the IBX fragment and the carbon nanotube surface also played a significant role. Indeed, the reagents bearing the longer spacer chains showed higher LF values and better oxidation performances. Regarding the LF values, the longer spacer may reduce steric hindrances for the IBA-functionalization of MWCNTs, increasing the number of groups involved in the multipoint covalent attachment [31]. Similarly, the better oxidation performance measured in the presence of the longer spacer was in accordance with previously reported data on the role that the spacer length can play for a certain mobility of the active species [32]. Interestingly, Au-MWCNTs based systems behaved as the more reactive reagents, thus justifying their increased electron-transfer properties ascribable to the presence of electroactive Au-carbon joints [33] in comparison with simple MWCNTs counterparts. The novel IBX supported reagents were easily recoverable from the reaction mixture, being successfully used for more runs after a simple reaction with the primary oxidant. These novel reagents can be applied in large scale processes, overcoming drawbacks associated with the use of oxidizing enzymes. Moreover, their metal-free structure, associated with the biocompatibility of MWCNTs, ensures novel reagents high eco-compatibility and low environmental impact.