Characterization of Food-Additive Titanium Dioxide and Dietary Exposure to Titanium Dioxide Nanoparticles among the Chinese Population

Titanium dioxide (TiO2) is a prevalent food additive, yet comprehensive data on particle size and dietary exposure are lacking in China. Transmission electron microscopy results revealed that the quantitative proportion of nanoparticles (NPs) in food-additive TiO2 was 37.7%, with a mass fraction of 9.89%. Laboratory test results showed that among the domestic products surveyed, candies excluding gum-based candies contained the highest content of TiO2. Using consumption data from the China Health and Nutrition Survey in 2018, the average dietary exposure for TiO2 and TiO2 NPs in the Chinese population were calculated at 34.84 and 3.44 μg/kg bw/day, respectively. The primary dietary sources were puffed food and powdered drinks. Exposure varied significantly across age and region, with children and Inner Mongolia residents having the highest intake. TiO2 NP exposure showed a negative correlation with age. Despite this, the dietary exposure risk of TiO2 NPs for the Chinese population remains deemed acceptable.


Introduction
Titanium dioxide (TiO 2 ) is widely used in various applications, including food, cosmetics, pharmaceuticals, and paints, primarily due to its coloring, photocatalytic, and biocidal properties [1,2].The European Union refers to food-additive TiO 2 as E171.E171 is found in higher concentrations in foods such as chewing gum, candies, and puddings, as it is commonly used as a coating for confectionery products [3][4][5].Nevertheless, recent years have seen a notable decline in its utilization, partly due to growing concerns regarding its safety [6].
Studies have suggested that nanoparticle (NPs) within TiO 2 particles may pose potential risks to human health.The nature and magnitude of the damage caused by TiO 2 NPs are influenced by their physical and chemical properties.Additionally, the reactivity and bioavailability of TiO 2 NPs are determined by these specific characteristics [7,8].Oral exposure to E171 can alter the gut microbiota of experimental animals, leading to changes in colon pH, significantly increasing oxidative stress and inflammatory responses [9,10].Multiple studies have demonstrated that following oral or inhalation exposure to TiO 2 NPs, accumulation can occur in vital organs such as the gastrointestinal system, lungs, heart, and liver [11,12].Furthermore, TiO 2 NPs have the potential to induce genotoxic effects including DNA damage or chromosomal instability [13][14][15], as well as cytotoxicity [16].This is mainly due to the production of reactive oxygen species, which leads to cellular damage, inflammation, genotoxicity, and adverse immune responses [17][18][19][20].
Consequently, regulatory agencies and industries in various nations have actively engaged in assessing and addressing the concerns surrounding the use and safety of TiO 2 .In 2021, the European Food Safety Authority (EFSA) released a report concluding that TiO 2 particles may induce DNA strand breaks and chromosomal damage, although they did not induce gene mutations.Consequently, the use of E171 as a food additive is no longer considered safe, and the sale of foods containing TiO 2 is banned in Europe [21].However, entities such as the Health Canada [22] and the UK Food Standards Agency [23] reviewed related research and found no evidence to suggest that dietary intake of food-grade TiO 2 was harmful to human health.Existing domestic research offers limited and outdated data on the systematic assessment of dietary intake of TiO 2 .
In this study, electron microscopy was utilized to characterize and examine foodadditive TiO 2 to obtain the percentage of NPs in both quantity and mass.The TiO 2 content in various food categories was determined using detection methods compliant with national food safety standards.The study aims to provide the most recent dietary exposure assessment results for TiO 2 and TiO 2 NPs in the Chinese population with updated consumption survey data.The differences in population characteristics and primary dietary sources were further identified.These findings are crucial for improving the regulatory framework for food-additive TiO 2 and prioritizing the protection of high-risk populations.

Study Design
This study chose food-additive TiO 2 samples available in domestic markets and analyzed particle size using electron microscopy.Statistical software and calculation formulas were employed to determine the numerical percentage and mass fractions of NPs.The TiO 2 content in food products sold in various provinces in China was detected using standard laboratory methods.In conjunction with consumption data from the Chinese Resident Food Consumption Survey in 2018, the daily exposure levels of TiO 2 in the Chinese population were assessed.The exposure risk of TiO 2 NPs was determined by multiplying the mass fraction of NPs.Furthermore, population characteristic differences were used to identify high-risk age groups, genders, and regions.Contribution rates of different food categories were analyzed to confirm the primary dietary sources.

Particle Size Distributions of Food-Additive TiO 2 by Transmission Electron Microscopy (TEM)
TiO 2 samples were from two leading companies in China that produce food-additive TiO 2 : Jiangsu Hushen Titanium White Technology Co., Ltd.(Jiangsu, China) and Tianjin Duofuyuan Industrial Co., Ltd.(Tianjin, China).A total of 10 samples from different production batches of each company were randomly selected.Additionally, a sample was provided by the China National Center for Food Safety Risk Assessment.The sources and batch numbers of the samples are specified in Table S1.
The TiO 2 powder was weighed to a precise mass of 10.00 ± 0.25 mg for each sample, followed by its transformation into a TiO 2 suspension through ultrasonic treatment.The size and morphology of the particles were characterized using TEM (model HT7700, Hitachi, Japan).TEM images of TiO 2 were obtained at parameters of an accelerating voltage of 10.0 kV, a working distance of 500 nm, and a magnification of 20,000.The procedure for the preparation of samples and the detection of particle size for food-additive TiO 2 is shown in Supplementary Material S1.The TEM images of maximum and minimum Feret diameters measured in the TiO 2 samples are shown in Figure S1.
To determine the quantitative proportions of NPs, the percentage of TiO 2 particles with a minimum Feret diameter smaller than 100 nm was calculated from the TEM image for each sample, using the ImageJ 1.53t software.Following the analysis methods for the identification and characterization of NPs in food additives developed by EFSA [24], we further determined the mass fraction of NPs.The short (a-axis) and long axes (c-axis) of the ellipse fitted to the 2D projection of each constituent particle were measured as proxies of their minimum and maximum Feret dimensions, respectively.Assuming that the particles are prolate ellipsoids (a-axis = b-axis < c-axis), the volume (V) of each particle was estimated as: The mass (M) of each particle was calculated as: ρ = the calculated bulk density of anatase TiO 2 = 3.89 g cm −3 (the crystal type of the TiO 2 samples supplied was anatase).
The mass fraction (M%) was given by the following formula: M NPs is the mass of NPs and M TiO2 is the mass of all TiO 2 particles.

Determination of TiO 2 Content by ICP-AES and DMC
Based on National Food Safety Standard-Standard for the Use of Food Additives (GB 2760-2014), we identified the food categories in which TiO 2 is used as a food additive.Specific categories surveyed included jams, preserved surface-drying fruit, preserved plum, fried nuts and seeds (TiO 2 is used in Chinese and European nut products primarily to enhance visual appeal by providing a bright white color, ensuring uniformity, and protecting against discoloration from light exposure.In China, common fried nuts and seeds include fried peanuts, charcoal roasted cashews, chia-chia melon seeds, etc.), cocoa products, chocolate and chocolate products, gum-based candy, other candies excluding gum-based candies, mayonnaise, salad dressing, powdered drinks (powdered drinks include soy milk powder, protein powder, coffee powder, etc.), jelly, and puffed food (puffed food includes potato chips, French fries, potpourri, shrimp chips, etc.).Corresponding products were chosen at random from markets in various provinces across the country.
The local Centers for Disease Control and Prevention were responsible for testing the levels of TiO 2 in food and beverages.The testing methods followed the National Food Safety Standard-Determination of Titanium Dioxide in Food (GB 5009.246-2016), which includes the Inductively Coupled Plasma-Atomic Emission Spectrometry (ICP-AES) method and the Diantipyryl Methane Colorimetry (DMC) method (the natural Ti content in food is extremely low, often below the detection limits of standard laboratory testing instruments.The detectable Ti levels in our tests are primarily due to the addition of food-additive TiO 2 .).The detection results under both testing methods showed no statistically significant difference.Specific testing methods and details can be found in Supplementary Material S2.

Dietary Exposure of TiO 2 and TiO 2 NPs
To assess dietary exposure to TiO 2 , we utilized the most recent consumption data available, which came from the Chinese Resident Food Consumption Survey in 2018 by the China National Center for Food Safety Risk Assessment.The survey was conducted in 32 urban survey sites in 18 provinces (autonomous regions and municipalities) in China.The target population was the Chinese population aged 3 years and above.A non-consecutive 3-day, 24 h retrospective method was utilized, aiming to collect comprehensive data on the consumption of various food items for each respondent.After excluding participants with missing or anomalous basic information, a total of 13,585 participants were included in the study, with the consumer group comprising 14.1% of the total.Powdered drinks had the highest average consumption, followed by jelly and puffed foods.The distribution of participant characteristics and consumption levels is provided in Tables S2 and S3.
The following equation is used to calculate daily intake of TiO 2 : EDI i (mg/kg bw/day) is the estimated daily intake of TiO 2 of individual i.F i,k is the amount of the kth food consumed by individual i which was averaged to grams per day over the 3 recording days (g/day).C k is the average content of TiO 2 in the kth food (mg/g).BW i is the body weight of individual i (kg), and n denotes the number of food categories.Due to the safety concerns surrounding TiO 2 NPs, we estimated the dietary exposure to TiO 2 NPs by multiplying the daily intake of TiO 2 by the mass fraction of NPs.
At present, there are no established recommended intake levels for food-additive TiO 2 in both domestic and international regulations.Therefore, the Margin of Safety (MoS) approach was used to assess the exposure risk of TiO 2 and TiO 2 NPs [25].The formula for calculating MoS values is as follows [25]: MoS is the Margin of Safety, with values ≥ 100 indicating that the dietary risk of foodadditive TiO 2 is within the acceptable range, while values < 100 indicate that TiO 2 poses a dietary risk to human health [26].NOAEL is the no observed adverse effect level for TiO 2 , and it was determined to be 1000 mg/kg BW/day based on the results of a reproductive developmental toxicity study [27].EDI is the dietary exposure to TiO 2 , and, in this study, the mean and P95 of exposure were used for the calculation.

Statistical Analysis
The Kruskal-Wallis rank-sum test was employed to analyze differences in daily intake of TiO 2 and TiO 2 NPs between groups using the mean.Linear regression analysis was used to assess the association between exposure levels and age.The statistical analyses were conducted by R 4.1.3.All statistical tests were 2-tailed, and differences were considered statistically significant when p < 0.05.

Size Distribution of Food-Additive TiO 2 and Proportion of TiO 2 NPs
As depicted in Figure 1, the TEM images revealed an irregular shape for food-additive TiO 2 .The TiO 2 particles within the size range of 100-125 nm exhibited the highest frequency, comprising 26.0% of the total particle count.Additionally, NPs with a minimum Feret diameter smaller than 100 nm accounted for 37.7%.
The statistical results in Table 1 showed that a total of 7647 particles were counted from 21 food-additive TiO 2 samples.The minimum Feret diameters of the particles contained in the 21 samples ranged from 26.8-291.7 nm, with mean values between 103.3-129.1 nm, and an average of 116.4 ± 38.5 nm.The maximum Feret diameters ranged from 33.6-413.6nm, with mean values between 124.6-159.2nm, and an average of 143.0 ± 49.8 nm.Based on the mass calculation formula for particles, the estimated mass fraction of nanoparticles was 9.89%.

Content Determination of TiO 2 in Food Categories
Table 2 presents the content of TiO 2 in 11 kinds of food categories detected by ICP-AES and DMC.The highest detection rate of TiO 2 was found in fried nuts and seeds (92.37%), while the lowest was in jam (21.74%).Three categories of food exceeded the permissible limits; namely, cocoa products, chocolate and chocolate products (1.87%), mayonnaise, salad dressing (1.85%), and puffed food (0.34%).The highest content was found in other candies excluding gum-based candies.On average, the content of TiO 2 measured 186.46 mg/kg, with a median of 0.75 mg/kg.Puffed food emerged as the second highest category, with the average and median values of TiO 2 content being 146.01 mg/kg and 0.86 mg/kg, respectively.Following closely were cocoa products, chocolate, and chocolate products, with an average content of 140.52 mg/kg for TiO 2 . 1 Considering the left-censored analysis data (i.e., results below the limit of detection (LOD)), an alternative approach was used based on recommendations from the World Health Organization [28] and the European Food Safety Authority [29].When the non-detection rate of TiO 2 in food samples was 60% or lower, non-detected values were replaced with 1/2 of the LOD.If the non-detection rate exceeded 60%, the LOD was used to replace the non-detected values. 2 The maximum use level for food-additive TiO 2 can be referenced in the National Food Safety Standard for Food Additive Use (GB2760-2014). 3 GMP means use in appropriate amount according to production needs.Through investigation of large enterprises in the powdered drink sector, it was found that only two enterprises in China currently use food-additive TiO 2 in their powdered drink products, and the maximum use level is 5 g/kg.Therefore, the maximum use level for powdered drinks in this assessment is calculated as 5000 mg/kg. 4The exceedance rate refers to the proportion of the number of food items in which the TiO 2 content in the surveyed samples exceeded the maximum use level specified in GB2760-2014. 5SD: Standard Deviation; P95: the 95th percentile; Min: minimum; Max: maximum.

Dietary Exposure of TiO 2 and TiO 2 NPs and Differences by Demographic Characteristics
Table 3 shows that the average exposure levels of TiO 2 and TiO 2 NPs in the total population were 34.84 µg/kg bw/day and 3.44 µg/kg bw/day, respectively.The P95 values were 161.24 µg/kg bw/day for TiO 2 and 15.94 µg/kg bw/day for TiO 2 NPs.Statistically significant differences were observed among distinct age groups (p < 0.001), with the average exposure levels ranking from highest to lowest for children, adolescents, adults, and the elderly.Notably, children displayed the highest intake, with average values of 90.27 µg/kg bw/day for TiO 2 and 8.93 µg/kg bw/day for TiO 2 NPs.Gender-based exposure comparisons revealed no significant variations (p = 0.846).The daily average intake of males was 33.90 µg/kg bw/day for TiO 2 and 3.35 µg/kg bw/day for TiO 2 NPs, while for females, the corresponding figures were 35.68 µg/kg bw/day for TiO 2 and 3.53 µg/kg bw/day for TiO 2 NPs.Furthermore, there were significant variations in intake among different provinces (p < 0.001), with Inner Mongolia exhibiting the highest exposure levels.The average exposure to TiO 2 for the Inner Mongolia population was 61.86 µg/kg bw/day, and for TiO 2 NPs, it was 6.12 µg/kg bw/day.The relationship between age and TiO 2 NP exposure was examined using linear regression analysis.The results indicated that the regression model was statistically significant (p < 0.001).The regression coefficient, which was less than 0, implied a negative correlation between age and TiO 2 NP exposure.This suggested that, as age increased, the exposure to TiO 2 NPs tended to decrease (Figure 2A).Based on Figure 2B, it was evident that puffed food and powdered drinks were the primary dietary sources of TiO 2 NP exposure across various age groups, with contributions ranging from 26.08% to 45.89% for puffed food and 23.81% to 50.82% for powdered drinks.Specifically, for children aged 3-9 years, their main dietary sources included puffed food (45.89%), powdered drinks (23.81%), and jams (14.74%).On the other hand, for adolescents aged 10-17 years, their primary dietary sources were powdered drinks (50.82%), puffed food (26.08%), and fried nuts and seeds (13.55%).Figure 3 displays a heat map of TiO 2 NP exposure in China.Participants residing in Inner Mongolia, Jiangsu, and Hebei regions had higher intakes of TiO 2 NPs compared to other areas.Among these regions, puffed food contributed the most to TiO 2 NP exposure for residents of Inner Mongolia, accounting for 91.16% of the exposure.This was followed by jams (5.68%) and mayonnaise, salad dressing (1.75%).

Discussion
The research findings indicated that in food-additive TiO2, the quantitative proportion and the mass fraction of NPs were 37.7% and 9.89%, respectively.Among products available in the Chinese market, other candies, excluding gum-based candies, had the highest content of TiO2.The average dietary exposure of the Chinese population to TiO2 and TiO2 NPs was 34.84 μg/kg bw/day and 3.44 μg/kg bw/day, respectively.Significant differences in dietary exposure were observed among different age groups and

Discussion
The research findings indicated that in food-additive TiO 2 , the quantitative proportion and the mass fraction of NPs were 37.7% and 9.89%, respectively.Among products available in the Chinese market, other candies, excluding gum-based candies, had the highest content of TiO 2 .The average dietary exposure of the Chinese population to TiO 2 and TiO 2 NPs was 34.84 µg/kg bw/day and 3.44 µg/kg bw/day, respectively.Significant differences in dietary exposure were observed among different age groups and regions (p < 0.001).Specifically, children aged 3-9 years and residents of Inner Mongolia had the highest average dietary intake.Linear regression analysis revealed a significant negative correlation between TiO 2 NP exposure and age, with children being at higher dietary exposure risk.The main dietary sources of TiO 2 NPs were puffed food and powdered drinks.MoS values indicated the dietary exposure risk of TiO 2 NPs for the Chinese population is acceptable.
The content of TiO 2 in food or beverages varies among different countries.Domestic research has found the highest concentration of TiO 2 in dried fig preserves (3409.3mg/kg), followed by chewing gum and chocolate beans.The content data were significantly higher than the detection data in this study, possibly due to a bias of the limited number of food items and sample size in the study by He et al. [30].A survey in Turkey revealed high concentrations of TiO 2 in candy products such as almond nougat (2400 mg/kg), cake decorations (2373 mg/kg), and syrups (2008 mg/kg) [26].In South Korea, ICP testing of titanium-containing candies found higher levels in chocolate, candies, and chewing gum, with the highest levels being 814 mg/kg, 355 mg/kg, and 42 mg/kg, respectively [31].TiO 2 is often used as a whitening agent added to candy coatings, so the TiO 2 content in candy products typically remains at relatively high levels [32].
Food-additive TiO 2 produced domestically and internationally was observed by TEM for the particle size distribution and the percentage of NPs.The findings of He et al. [30], which detected NPs in food-additive TiO 2 in China with quantitative proportion and mass fractions of 34.7% and 10.87%, respectively, were close to the results of our study.The EFSA report in 2016 on the particle size analysis of commercially available TiO 2 samples under TEM found that the particle diameter ranged from 80-180 nm, with a median diameter of 113 nm.The mass percentage range of nanoparticles was from 0.0% to 3.2%.Approximately 36% of the particles had a diameter smaller than 100 nm [33].In the 2022 Australia and New Zealand report, it was mentioned that for E171-E, which had the highest nanoparticle content, the median particle size was 104 nm.Approximately 36-45% of the particles had a diameter less than 100 nm [34].It can be observed that the quantitative proportion of TiO 2 NPs ranges from 34% to 45%, while the mass fraction may be influenced by the particle crystalline structure and its corresponding density.
TiO 2 NPs have the potential to accumulate in the body, but their oral bioavailability is low.Following oral ingestion, TiO 2 NPs are primarily distributed in the liver, spleen, and lungs [35,36].Studies conducted by Kreyling et al. [36] involved administering TiO 2 NP suspensions to rats via gavage and observed a slow process of TiO 2 NP absorption and elimination.Individuals exposed to TiO 2 NPs over the long term may experience particle accumulation in specific cells and organs.In the study of TiO 2 NPs given by gavage to rats the highest Ti concentration was detected in the liver, followed by the spleen and small intestine.The oral bioavailability was found to be approximately 0.00021% [37].Furthermore, research by Chaudhry et al. indicated that the accumulation of TiO 2 NPs in organs following oral exposure depends on the age of the exposed individuals.Younger individuals have higher intestinal permeability, leading to increased absorption of TiO 2 NPs [38].
The daily intake of TiO 2 NPs is influenced by food categories and eating habits in different countries, with higher exposure risks for children.A study investigated the intake of TiO 2 and its NPs in the Dutch population aged 2 to over 70 years from food, food supplements, and toothpaste.The estimated average intake of TiO 2 NPs was 0.19 µg/kg bw/day for the elderly, 0.55 µg/kg bw/day for those aged 7-69, and 2.16 µg/kg bw/day for toddlers.Products contributing most to TiO 2 dietary intake included toothpaste (limited to toddlers) and candies [39].An Italian study found that the daily intake of TiO 2 NPs through chewing gum among the European population ranged from 0.28 to 112.40 mg/kg bw/day, with children ingesting more nano-TiO 2 than adolescents and adults [40].In 2016, EFSA estimated the dietary intake of NPs at a mass fraction of 3.2%.In a non-brand-loyal exposure scenario, the average daily exposure for infants, adolescents, adults, and the elderly was 0.01 mg/kg bw/day, while children had a daily exposure of 0.18 mg/kg bw/day.Among these groups, 3-9-year-old children had the highest average exposure, with their main dietary sources being candies, sauces, fine bakery wares, and salads [33].The reasons for the differences in exposure levels between children and adults may be attributed to two factors.First, products containing high levels of food-additive TiO 2 , such as candies, cakes, and other sweets, are highly favored by children.Second, children consume more food than adults when measured in kilograms of body weight [41].
However, in this study, the primary dietary sources of TiO 2 NP exposure for the Chinese population were found to be puffed food and powdered drinks rather than candies.This difference can be attributed to the food consumption data used in this study.We compared the results of dietary exposure to TiO 2 for Chinese residents based on the consumption data of the China Health and Nutrition Survey in 2011.Their results indicated significantly higher exposure levels compared to the results of this study.Moreover, sweet foods (such as sugar, chocolate, and cakes) were identified as the largest contributors to TiO 2 NP intake, accounting for at least 65% of the total intake [30].A study examining snack consumption patterns in the central and western regions of China observed that approximately 40% of primary and middle school students consume puffed food, with nuts, candies, and chocolates following closely [42].We reasonably speculate that the consumption of sweet foods in China may have decreased in recent years, while the consumption of puffed food and nut-based products has gradually increased.
Although this study tested a wide range of food categories where the addition of TiO 2 is permitted in China and conducted the latest dietary exposure assessment, there are still some limitations to consider.First, the study did not take into account TiO 2 in food supplements or food packaging materials.However, the impact of such sources is likely to be minimal due to low concentrations and low consumption populations.Secondly, other exposure routes such as dermal exposure and inhalation were not included in the analysis, despite the widespread use of TiO 2 in cosmetics, personal care products, and other applications.Once the toxic effects and safety limits of TiO 2 NPs are determined, a multiroute risk assessment will be necessary.Lastly, for the assessment of TiO 2 intake in the Chinese population, it is important to incorporate updated consumption survey data.This will enable real-time monitoring of exposure dynamics and facilitate timely implementation of population intervention measures and regulation of TiO 2 as a food additive.

Conclusions
The study represents the first comprehensive assessment of various food categories permitted to be added with TiO 2 in the Chinese domestic market, providing the latest exposure assessment for TiO 2 and its NPs in the Chinese population.The highest content of TiO 2 was found in other candies excluding gum-based candies.Based on the mass fraction of NPs in food-additive TiO 2 (9.89%), the estimated average dietary exposure for TiO 2 and TiO 2 NPs in the Chinese population was 34.84 µg/kg bw/day and 3.44 µg/kg bw/day, respectively.Children aged 3-9 years and residents of Inner Mongolia face the highest exposure risks.However, the estimated intake of TiO 2 NPs in this study was far below the levels that could potentially cause harm.

Supplementary Materials:
The following supporting information can be downloaded at: https: //www.mdpi.com/article/10.3390/nano14171427/s1,Table S1.Sources and weighing of food additive TiO 2 samples.Figure S1.Maximum and minimum Feret diameters measured on TiO 2 samples under TEM.The particle size of each particle was determined by ImageJ 1.53t software.Table S2.Number and percentage of Chinese consumers by demographic characteristics in 2018.Table S3.Consumption of different food categories in Chinese consumers in 2018 (g/day) 1 .

Figure 1 .
Figure 1.The frequency histogram of particle size distribution of food-additive TiO2 samples.The quantitative proportion of TiO2 NPs was 37.7%.The TEM images of food-additive TiO2 particles showed the primary particle distribution by TEM (A) and particle size analysis by ImageJ (B) of the TiO2 sample.

Figure 1 .
Figure 1.The frequency histogram of particle size distribution of food-additive TiO 2 samples.The quantitative proportion of TiO 2 NPs was 37.7%.The TEM images of food-additive TiO 2 particles showed the primary particle distribution by TEM (A) and particle size analysis by ImageJ (B) of the TiO 2 sample.

Nanomaterials 2024 , 14 Figure 2 .
Figure 2. (A) The scatter plot of age and TiO2 NP exposure in the Chinese population.The linear regression curve equation was given as y = −0.1006x+ 7.3345.(B)The contribution of different food categories to TiO2 NP exposure in various age groups.Puffed food and powdered drinks were identified as the primary dietary sources.

Figure 2 .
Figure 2. (A) The scatter plot of age and TiO 2 NP exposure in the Chinese population.The linear regression curve equation was given as y = −0.1006x+ 7.3345.(B) The contribution of different food categories to TiO 2 NP exposure in various age groups.Puffed food and powdered drinks were identified as the primary dietary sources.

Nanomaterials 2024 , 14 Figure 3 .
Figure 3.The heat map of TiO2 NP exposure in China.Deeper shades of blue on the map indicated higher dietary exposure to TiO2 NPs.The pie chart represented the contribution of different food categories to TiO2 NP exposure in the Inner Mongolia region.

Figure 3 .
Figure 3.The heat map of TiO 2 NP exposure in China.Deeper shades of blue on the map indicated higher dietary exposure to TiO 2 NPs.The pie chart represented the contribution of different food categories to TiO 2 NP exposure in the Inner Mongolia region.

Table 1 .
Statistical results of particle size of food-additive TiO 2 samples 1 .

Table 1 .
Statistical results of particle size of food-additive TiO2 samples 1 .

Table 2 .
Content of TiO 2 in different food categories1.

Table 3 .
Dietary exposure of TiO 2 and TiO 2 NPs by demographic characteristics in the Chinese population (µg/kg bw/day) 1 .

Table 4
presents the MoS values for different age groups.Under the NOAEL of 1000 mg/kg bw/day, the calculated lowest MoS was higher than 100, indicating a low safety risk.

Table 4 .
MoS values calculated based on the exposure estimated in six population groups1.Age and Regional Distribution and Food Contribution of TiO 2 NP Exposure