Presence of Heavy Metals in Irrigation Water, Soils, Fruits, and Vegetables: Health Risk Assessment in Peri-Urban Boumerdes City, Algeria

This study investigates heavy metal contamination in soils, irrigation water, and agricultural produce (fruits: Vitis vinifera (grape), Cucumis melo var. saccharimus (melon), and Citrullus vulgaris. Schrade (watermelon); vegetables: Lycopersicum esculentum L. (tomato), Cucurbita pepo (zucchini), Daucus carota (carrot), Lactuca sativa (lettuce), Convolvulus Batatas (potato), and Capsicum annuum L. (green pepper)) in the Boumerdes region of Algeria. The concentrations of seven heavy metals (cadmium (Cd), chromium (Cr), copper (Cu), iron (Fe), nickel (Ni), lead (Pb), and zinc (Zn)) in soil and food samples were analyzed using atomic absorption spectrometry. Health risks associated with these metals were evaluated through the estimated daily intake (EDI), non-carcinogenic risks (using target hazard quotient (THQ), total target hazard quotient (TTHQ), and hazard index (HI)), and carcinogenic risks (cancer risk factor (CR)). Statistical analyses, including cluster analysis (CA) and Pearson correlation, were conducted to interpret the data. The results revealed the highest metal transfer as follows: Cd was most significantly transferred to tomatoes and watermelons; Cr to carrots; Cu to tomatoes; and Fe, Ni, Pb, and Zn to lettuce. Among fruits, the highest EDI values were for Zn (2.54·10−3 mg/day) and Cu (1.17·10−3 mg/day), with melons showing the highest Zn levels. For vegetables, the highest EDI values were for Fe (1.68·10−2 mg/day) and Zn (8.37·10−3 mg/day), with potatoes showing the highest Fe levels. Although all heavy metal concentrations were within the World Health Organization’s permissible limits, the HI and TTHQ values indicated potential health risks, particularly from vegetable consumption. These findings suggest the need for ongoing monitoring to ensure food safety and mitigate health risks associated with heavy metal contamination.


Introduction
The contamination of the food chain by heavy metals (Cd, Cu, Ni, Pb, Zn, etc.) is considered one of the main environmental routes of human exposure leading to a potential health risk.The translocation of these metals in the food chain is one of the consequences of their presence in soils and environmental water [1][2][3][4].Since heavy metals are nonbiodegradable, persistent in a natural environment, and mobile, they can easily enter the human body with food and accumulate in different parts of the body, causing, even at low concentrations, adverse health effects [5][6][7][8].Heavy metals such as cadmium and lead are one of the most toxic metals with carcinogenic and mutagenic activities [9,10].Cadmium exposure can result in serious systemic diseases and damage to the skeletal and cardiovascular systems, the kidneys, and the liver, as well as problems with seeing and hearing [10,11].Lead is a highly poisonous metal affecting many organ systems, especially the nervous, respiratory, digestive, and reproductive systems, and prevents enzymes from performing their normal activities.Continuous exposure of the body (e.g., ingestion and skin contact with highly contaminated soil and/or dust) leads to a decline in neurocognitive functions, nephrotoxicity, hypertension, decreased reproductivity, cataract formation, hearing defects, dental problems, and mucocutaneous signs [7,[12][13][14].In addition, the ingestion of heavy metals can seriously cause a depletion of certain essential nutrients in the body, which in turn causes a decrease in immunological defenses, intrauterine growth retardation, psychosocial dysfunctions, disabilities associated with malnutrition, and a high prevalence of upper gastrointestinal cancer [9].It was found that kidney stone incidence increases with a high Pb exposure in males and with Pb and Cd co-exposure in males and females [15].
The presence of heavy metals in the environment may result from natural processes (e.g., weathering of metal-containing rocks, volcanic eruptions, forest fires) or human activities [8].The main anthropogenic sources of heavy metals are mining, the metallurgical industry, fertilizers and pesticides applied in soil cultivation, medical waste, factory and car engine emissions, incinerators and ashes from waste incineration, municipal landfills, wastewater effluents, and sewage sludge [16,17].When the metals are released from their sources, they enter the air, water, and soil and are transported into the environment together with aerosols, surface water and groundwater, suspended solids, or bottom sediments.Both the concentration of individual metals and their total concentration in each part of the environment are crucial to determine their impact on crops and the human organism.The availability of the metal to living organisms is mainly controlled by the dissolution of the carrier minerals and then by the adsorption and/or precipitation of this metal as a result of the geochemical reactions taking place in aerosol, water, or sediment [18,19].The metals released in this way accumulate in plants grown in polluted areas, which enter the body of the consumers as food.Usually, fruits and vegetables are considered the healthiest for the consumer's body; however, their cultivation place and method determine whether they provide the body with the proper ingredients [3,20].
This study aimed to determine the potential health risks of consuming popular fruits (grape, melon, and watermelon) and vegetables (tomato, zucchini, carrot, lettuce, potato, and green pepper) grown in four Algerian peri-urban regions (Boudouaou, Corso, Dellys, and Naceria) localized near Boumerdes city.This area is one of the most important areas in the production of agricultural products in Algeria.In order to compensate for the hydric shortages, the crops are irrigated with groundwater, river water, or treated sewage and wastewater from the municipal sewage treatment plant [21].Both ground-and surface water are exposed to leachates from these landfills, and wastewater can be a potential source of hazardous elements in the crops.To achieve this goal, the concentrations of seven heavy metals (copper, zinc, iron, chromium, cadmium, nickel, and lead) in the river and groundwaters, soils, and fruits and vegetables were determined, and the transfer factor of each heavy metal from different agricultural activities was established.To date, few papers have described studies of the environmental quality in the Boumerdes region [21][22][23]; however, none of them focused on assessing the risk of heavy metals in the crops grown there.The knowledge of the heavy metal content in nationally representative samples of food produced in the Boumerdes region is extremely important in terms of the environmental effect on agriculture, the quality of crops, and consumers' health.

Metal Concentration in Irrigation Water
The pH of the water used for the crop irrigation decreased in the order of Boudouaou (pH = 7.2) > Corso (pH = 7.0) > Naceria (pH = 6.7) > Dellys (pH = 5.5).The mean concentration values of heavy metals determined in the groundwater of the Boudouaou region and the river water of the Corso, Naceria, and Dellys regions and the maximum levels (MLs) recommended for irrigation water by The Food and Agriculture Organization of the United Nations and International Water Management Institute (FAO/IWMI) [24] and the United States Environmental Protection Agency (USEPA) [25] are presented in Table 1.No carcinogenic cadmium was found in any tested waters in concentrations above the detection limit (LOD) of 0.5 µg/L.Chromium concentrations below the LOD of 3 µg/L were found only in river water applied in Corso and Naceria farmlands and one groundwater applied for zucchini irrigation in Boudouaou farmland.Other waters contained from 0.09 to 0.26 mg Cr/L, with values exceeding the ML for irrigation waters for carrot and green pepper cultivations.The concentration of copper in all tested waters was above the recommended value and ranged from 0.56 to 1.17 mg/L.The ML value was also exceeded for nickel concentration in the waters for the irrigation of zucchini (0.87 mg/L), potato (0.91 mg/L), and tomato (1.98 mg/L).The concentrations of Pb, Fe, and Zn were below the recommended value.The Pb content ranged from 0.03 to 0.82 mg/L, with noticeably higher values recorded in the water for the irrigation of lettuce and potato in the Dellys region (0.82 and 0.42 mg/L, respectively).The highest values were for water used to irrigate green peppers in the Dellys region (0.26 mg/L) and carrots in the Boudouaou region (0.19 mg/L).In turn, the concentrations of Fe and Ni changed from 0.04 (Naceria, watermelon irrigation) to 1.10 mg/L (Dellys, lettuce irrigation) and from 0.05 (Corso, grape irrigation) to 1.02 mg/L (Boudouaou, zucchini irrigation).Taking into account the total content of heavy metals analyzed (in mg/L), the purity of the water used to irrigate crops was the highest in the Naceria region (0.86) and decreased in the following series: Corso (2.35); Boudouaou (9.28); and Dellys (9.80).
From the comparison of the obtained results with the literature data (Figure 1), it can be seen that the tested waters of the Boumerdes region used in crop irrigation definitely contain more copper, lead, and nickel than the waters of other regions of Algeria [26][27][28][29][30][31][32].In turn, the iron content was comparable to the range of this element in the waters of Reghaia Lak [26] and several times lower than that in the waters in other regions [27,28,31].Zinc concentrations were like those in the waters of Oued Es-Souk [28] and El Harrach River [29], except for Djendjen River [32], with several times higher concentrations of this element, and Ain Azel [33], with a zinc content of 30 mg/kg.The tested waters have a significantly lower chromium content (ca.0.26 mg/L) compared to other Algerian waters but a higher iron content with 0.04 to 1.10 mg Fe per 1 L of water.irrigation) to 1.02 mg/L (Boudouaou, zucchini irrigation).Taking into account the total content of heavy metals analyzed (in mg/L), the purity of the water used to irrigate crops was the highest in the Naceria region (0.86) and decreased in the following series: Corso (2.35); Boudouaou (9.28); and Dellys (9.80).
From the comparison of the obtained results with the literature data (Figure 1), it can be seen that the tested waters of the Boumerdes region used in crop irrigation definitely contain more copper, lead, and nickel than the waters of other regions of Algeria [26][27][28][29][30][31][32].In turn, the iron content was comparable to the range of this element in the waters of Reghaia Lak [26] and several times lower than that in the waters in other regions [27,28,31].Zinc concentrations were like those in the waters of Oued Es-Souk [28] and El Harrach River [29], except for Djendjen River [32], with several times higher concentrations of this element, and Ain Azel [33], with a zinc content of 30 mg/kg.The tested waters have a significantly lower chromium content (ca.0.26 mg/L) compared to other Algerian waters but a higher iron content with 0.04 to 1.10 mg Fe per 1 L of water.

Metal Concentration in Soils
The pH and mean values of the metal concentrations in the studied soils are presented in Table 2. Soil pH is the main variable influencing biological, chemical, and physical properties and plant growth.It was found that even the smallest amount of acid causes a change in the pH, resulting in the increased mobility of metals in the soil environment [34].The studied soils are weakly alkaline, from neutral to weakly acidic.The pH of 6.32 and 6.57 for the soils of the Dellys (lettuce cultivation) and Boudouaou regions (zucchini cultivation), respectively, may affect the mobility of heavy metals present in these soils.

Metal Concentration in Soils
The pH and mean values of the metal concentrations in the studied soils are presented in Table 2. Soil pH is the main variable influencing biological, chemical, and physical properties and plant growth.It was found that even the smallest amount of acid causes a change in the pH, resulting in the increased mobility of metals in the soil environment [34].The studied soils are weakly alkaline, from neutral to weakly acidic.The pH of 6.32 and 6.57 for the soils of the Dellys (lettuce cultivation) and Boudouaou regions (zucchini cultivation), respectively, may affect the mobility of heavy metals present in these soils.
The concentrations of heavy metals analyzed in the agricultural soils are below the maximum limits (MLs) authorized by the European Commission [35] and the State Administration of Market Regulation (for Fe) [36] (Table 2).Among the tested metals, significantly higher concentrations were recorded for Cu, Fe, Pb, and Zn than for Cd, Cr, and Ni.Cadmium was not found in amounts above the LOD in Dellys soils and Boudouaou soils with carrot cultivation.In the other soils, the concentration of this element ranged from 20 to 90 µg/kg.Chromium has been detected in amounts from 2.24 mg/kg in Boudouaou soil (carrot cultivation) to 12.17 mg/kg in Corso soil (melon cultivation); nickel-from 3.25 mg/kg in Boudouaou (carrot cultivation) to 21.25 mg/kg in Corso (grape cultivation); and lead-from 49.22 mg/kg in Naceria soil to 110.25 mg/kg in Dellys soil (potato cultivation).The Cu and Fe contents were the lowest in Dellys soil (potato cultivation; 38.25 and 42.74 mg/kg, respectively) and the highest in Boudouaou (zucchini cultivation; 78.00 mg/kg) and Dellys (potato cultivation; 120.42 mg/kg), respectively.The Zn concentration ranged from 70.12 mg/kg for Naceria soil to 108.21 mg/kg for Dellys soil (lettuce cultivation).Ghemmit-Doulache [23] determined the concentrations of these metals in 1 kg of soil irrigated by treated wastewater from the Treatment Station Treated Wastewater of Boumerdes at the levels of 0.0275 mg of Cd; 50.53 mg of Cr; 18.03 mg of Cu; 12.92 mg of Ni; 23.28 mg of Pb; and 69.65 mg of Zn.For the determination of the correlation between the soil pH and heavy metal concentrations in the soils, Pearson coefficients were calculated and are listed in Table 3.The soil pH does not correlate significantly with the studied metal concentration.In turn, Fe has a significant negative correlation with Cd, Cr, and Ni, indicating that an increase in the Fe concentration in the soil leads to a decrease in the Cd, Cr, and Ni concentrations.Additionally, a significant positive correlation between Cd, Cr, and Ni and Fe, Pb, and Zn has been observed, which suggests that these metal groups originated from similar anthropogenic sources.It is difficult to clearly indicate the significant impact of the industrial activities indicated in Section 3.1 on the quality of the tested soils; therefore, the reasons for the presence of heavy metals in these soils should be sought in municipal wastes and the pesticides and/or mineral fertilizers used.The bioavailability of Cd, Cu, Zn, and Pb present in the soil from the peri-urban region of New Delhi also showed a lack of relationship with the soil pH [37].
As shown in Figure 2, the dendrogram resulting from the hierarchical cluster analysis (CA) indicates the presence of two main clusters with subclusters.The first cluster containing Pb, Zn, and Fe confirms a good correlation between these metals, with a closer relation between Zn and Fe.The second cluster containing Cd, Cr, Ni, Cu, and soil pH indicates a good correlation between Ni and Cr, a weaker correlation between this metal pair and Cd, and no correlation with Cu.In turn, the Cu concentration correlated well only with the pH of the soil.The soil pH does not correlate significantly with the studied metal concentration.In turn, Fe has a significant negative correlation with Cd, Cr, and Ni, indicating that an increase in the Fe concentration in the soil leads to a decrease in the Cd, Cr, and Ni concentrations.Additionally, a significant positive correlation between Cd, Cr, and Ni and Fe, Pb, and Zn has been observed, which suggests that these metal groups originated from similar anthropogenic sources.It is difficult to clearly indicate the significant impact of the industrial activities indicated in Section 3.1 on the quality of the tested soils; therefore, the reasons for the presence of heavy metals in these soils should be sought in municipal wastes and the pesticides and/or mineral fertilizers used.The bioavailability of Cd, Cu, Zn, and Pb present in the soil from the peri-urban region of New Delhi also showed a lack of relationship with the soil pH [37].
As shown in Figure 2, the dendrogram resulting from the hierarchical cluster analysis (CA) indicates the presence of two main clusters with subclusters.The first cluster containing Pb, Zn, and Fe confirms a good correlation between these metals, with a closer relation between Zn and Fe.The second cluster containing Cd, Cr, Ni, Cu, and soil pH indicates a good correlation between Ni and Cr, a weaker correlation between this metal pair and Cd, and no correlation with Cu.In turn, the Cu concentration correlated well only with the pH of the soil.When comparing the obtained results with the literature data (Figure 3), it can be concluded that the tested soils of all regions of Boumerdes contain less cadmium, chromium, and nickel but more lead than the soils of other regions of Algeria [33,[38][39][40][41][42][43].In turn, the copper content was comparable to the range of this element in the soils of the Mitidja plain [38], Annaba [39], and Setif city [41] and several times higher than in soils in other regions.The zinc concentrations were like those in soils in other regions, except for Tlemcen province [43], with four times higher concentrations of this element, and Ain Azel [33], with a zinc content of 30 mg/kg.The tested soils have a significantly lower iron When comparing the obtained results with the literature data (Figure 3), it can be concluded that the tested soils of all regions of Boumerdes contain less cadmium, chromium, and nickel but more lead than the soils of other regions of Algeria [33,[38][39][40][41][42][43].In turn, the copper content was comparable to the range of this element in the soils of the Mitidja plain [38], Annaba [39], and Setif city [41] and several times higher than in soils in other regions.The zinc concentrations were like those in soils in other regions, except for Tlemcen province [43], with four times higher concentrations of this element, and Ain Azel [33], with a zinc content of 30 mg/kg.The tested soils have a significantly lower iron content (ca.80 mg/kg) compared to other Algerian soils, with 16.7 to 37 g Fe per 1 kg soil in the Tlemcen province [43] and Mitidja plain [38], respectively.content (ca.80 mg/kg) compared to other Algerian soils, with 16.7 to 37 g Fe per 1 kg soil in the Tlemcen province [43] and Mitidja plain [38], respectively.

Metal Concentration in Fruits and Vegetables
The average concentrations of heavy metals found in fresh fruits and vegetables sampled in the studied area are summarized in Table 4.
Cadmium and chromium were not detected in the studied food in concentrations over the LOD value of 0.013 mg/kg, except in carrot, in which 0.090 mg of Cr per 1 kg was found.The contents of other metals varied as follows: from 0.94 to 13.01 mg/kg (in grape and tomato, respectively) for Cu; from 0.94 to 54.20 mg/kg (in grape and lettuce, respectively) for Fe; from <0.68 to 1.01 mg/kg (in grape, melon, zucchini, and green pepper and in lettuce, respectively) for Ni; from <0.42 to 3.45 mg/kg (in melon and watermelon and in lettuce, respectively) for Pb; and from 1.33 to 19.52 mg/kg (in grape and lettuce, respectively) for Zn.In most cases, the metal concentrations in the tested foods were below the maximum level (ML) recommended by the Joint World Health Organization and Food and Agriculture Organization of the United Nations/WHO Food [44], except lead, which was present in all tested fruits and vegetables in amounts exceeding the recommended safety limit of 0.1 mg/kg.

Metal Concentration in Fruits and Vegetables
The average concentrations of heavy metals found in fresh fruits and vegetables sampled in the studied area are summarized in Table 4. Cadmium and chromium were not detected in the studied food in concentrations over the LOD value of 0.013 mg/kg, except in carrot, in which 0.090 mg of Cr per 1 kg was found.The contents of other metals varied as follows: from 0.94 to 13.01 mg/kg (in grape and tomato, respectively) for Cu; from 0.94 to 54.20 mg/kg (in grape and lettuce, respectively) for Fe; from <0.68 to 1.01 mg/kg (in grape, melon, zucchini, and green pepper and in lettuce, respectively) for Ni; from <0.42 to 3.45 mg/kg (in melon and watermelon and in lettuce, respectively) for Pb; and from 1.33 to 19.52 mg/kg (in grape and lettuce, respectively) for Zn.In most cases, the metal concentrations in the tested foods were below the maximum level (ML) recommended by the Joint World Health Organization and Food and Agriculture Organization of the United Nations/WHO Food [44], except lead, which was present in all tested fruits and vegetables in amounts exceeding the recommended safety limit of 0.1 mg/kg.
The content of the tested metals in individual crops differed depending on the plant and was lower in the case of fruits.The total content (in mg/kg) of all tested heavy metals in fruits and vegetables increased in the following order: watermelon ∼ = grape < melon < zucchini < tomato < green pepper < carrot < potato < lettuce.The high content of heavy metals in lettuce is attributed to its physiology.The large leaf area and high rate of transpiration and plant growth may have a key impact on the ability to absorb heavy metals from water and soil and to accumulate them in the plant [45].Rutigliano et al. [46] identified higher amounts of Cd, Cr, and Ni in lettuce Lactuca sativa than in pumpkin Cucurbita pep.grown in Southern Italy.The Pb content of the tested tomatoes (1.11 mg/kg) was slightly higher than that of tomatoes grown in North-East Algeria (Jijel, 0.82 mg/kg) [47] or Pakistan (0.98 mg/kg) [48] but higher than in Polish tomatoes (<0.004-0.022mg/kg fresh mass) [49].However, in the tested tomatoes and Polish tomatoes [49], no cadmium was detected in amounts above 0.047 mg/kg, while Bounar et al. [47] and De Sousa et al. [48] found the presence of this element in amounts above the permissible level (0.45 and 0.66 mg/kg, respectively).
The Pearson coefficient values listed in Table 5 (Cd and Cr were not considered) were calculated to identify the correlation between the elements in the studied fruit and vegetables.A significant positive correlation was observed in the following metal pairs: Fe-Ni (r = 0.832), Fe-Zn (r = 0.896), and Ni-Zn (r = 0.841) at p < 0.01, Fe-Pb (r = 0.767) and Pb-Zn (r = 0.791) at p < 0.02, and Ni-Pb (r = 0.693) at p < 0.05.This means that Ni, Zn, and Pb concentrations may increase with increasing Fe content, Zn and Pb with increasing Ni content, and Zn with increasing Pb content, and vice versa, and indicates that these metals are mutually associated, commonly interact, and have a common source of pollution [50].
The CA results presented as the dendrogram in Figure 4 show one main cluster with Pb, Ni, Zn, and Fe, indicating a good correlation between these metals, with a closer relation between Zn and Fe.Cu is outside this cluster and correlates poorly with the other metals.In turn, comparing the amounts of metals in food and in the respective soils where it was cultivated, a significant correlation (p < 0.01) was found only in the case of iron (r = 0.833) and zinc (r = 0.823).For the other metal pairs, non-significant correlations were found, meaning that the content of one metal does not influence other metals' contents in the studied soils and food.It suggests that the presence of these metals in food results from human activities carried out directly on the researched agricultural fields or in their surroundings.

Transfer Factor
The heavy metal transfer from soil to plants is one of the main routes of human exposure to metals through the food chain.This phenomenon can be expressed numerically by calculating the transfer factor (TF) value from Equation (1).The TF is defined as the migration of metals from the soil to the edible part of the plants, making them available for consumption [51,52].In this study, the TF was determined for the economically valuable parts of the plants.Several physicochemical factors of the soil and plant species govern this function.As can be seen from Figure 5, the TF values for fruits and vegetables grown at four studied sites varied in a wide range (0 and 0.040 for Cr; 0.018 and 0.196 for Cu; 0.013 and 0.515 for Fe; 0.009 to 0.193 for Ni; 0.005 to 0.050 for Pb; and 0.016 and 0.180 for Zn), depending on the type of plant and its place of growth.Cadmium was not transferred from the soil to the studied food.In turn, comparing the amounts of metals in food and in the respective soils where it was cultivated, a significant correlation (p < 0.01) was found only in the case of iron (r = 0.833) and zinc (r = 0.823).For the other metal pairs, non-significant correlations were found, meaning that the content of one metal does not influence other metals' contents in the studied soils and food.It suggests that the presence of these metals in food results from human activities carried out directly on the researched agricultural fields or in their surroundings.

Transfer Factor
The heavy metal transfer from soil to plants is one of the main routes of human exposure to metals through the food chain.This phenomenon can be expressed numerically by calculating the transfer factor (TF) value from Equation (1).The TF is defined as the migration of metals from the soil to the edible part of the plants, making them available for consumption [51,52].In this study, the TF was determined for the economically valuable parts of the plants.Several physicochemical factors of the soil and plant species govern this function.As can be seen from Figure 5, the TF values for fruits and vegetables grown at four studied sites varied in a wide range (0 and 0.040 for Cr; 0.018 and 0.196 for Cu; 0.013 and 0.515 for Fe; 0.009 to 0.193 for Ni; 0.005 to 0.050 for Pb; and 0.016 and 0.180 for Zn), depending on the type of plant and its place of growth.Cadmium was not transferred from the soil to the studied food.Among the studied metals, the highest transfer of Cr to carrot, Cu to tomato, and Fe, Ni, Pb, and Zn to lettuce was observed.This variation can be explained by the differences in the concentrations of metals in the soils, the selective absorption of the elements by the plants [53], and the transmission in the individual plant tissues.The tested fruits absorbed significantly fewer metals than vegetables.The mean TF values for the studied vegetables decrease in the order Fe > Zn = Cu > Ni > Pb > Cr > Cd, similar to that obtained by Wang et al. [54] (except Cd).The relatively lower transfer factor values for Pb and Cr can be attributed to the stronger binding of these metals to the soil [47].The results indicate that lettuce is the biggest accumulator of heavy metals, especially Fe, Ni, and Zn.On the other hand, the transfer factor values show that certain vegetables (e.g., zucchini) reduce the risk of human exposure to metal contamination in the soil [55].

Estimated Daily Intake of Heavy Metals
In being aware of the presence of heavy metals in the tested food and their adverse effects, it is necessary to determine the level of exposure of the body to these pollutants.The estimated daily intake (EDI) of the analyzed metals was calculated from Equation ( 2), according to the mean concentration of each metal in each food and the respective consumption rates.The EDI for individual food, total EDI for groups of the studied fruits and vegetables and all food, and maximum tolerable daily intake (MTDI) of the studied metals from the consumption of fruits and vegetables [56] are shown in Table 6.The highest total daily intake for all fruits was found for Zn (2.54•10 −3 mg/day) and Cu (1.17•10 −3 mg/day), with the highest EDI for melon (8.37•10 −4 and 6.92•10 −4 mg/day, respectively), whilst among the vegetables, the total daily intake was the highest for Fe (1.68•10 −2 mg/day) and Zn (8.37•10 −3 mg/day), with the highest EDI for potatoes (8.21•10 −3 and 3.51•10 −3 mg/day, respectively).In turn, the exposure to Cd and Cr was characterized by the lowest EDI values considering both the fruit alone, the vegetables alone, and their total.The total daily intakes of individual metals from all studied food were much lower than the MTDI values and constituted below 0.1% of this value for Cd, Cr, Cu, and Zn, 0.2% for Ni, and 0.6% for Pb.Among the studied metals, the highest transfer of Cr to carrot, Cu to tomato, and Fe, Ni, Pb, and Zn to lettuce was observed.This variation can be explained by the differences in the concentrations of metals in the soils, the selective absorption of the elements by the plants [53], and the transmission in the individual plant tissues.The tested fruits absorbed significantly fewer metals than vegetables.The mean TF values for the studied vegetables decrease in the order Fe > Zn = Cu > Ni > Pb > Cr > Cd, similar to that obtained by Wang et al. [54] (except Cd).The relatively lower transfer factor values for Pb and Cr can be attributed to the stronger binding of these metals to the soil [47].The results indicate that lettuce is the biggest accumulator of heavy metals, especially Fe, Ni, and Zn.On the other hand, the transfer factor values show that certain vegetables (e.g., zucchini) reduce the risk of human exposure to metal contamination in the soil [55].

Estimated Daily Intake of Heavy Metals
In being aware of the presence of heavy metals in the tested food and their adverse effects, it is necessary to determine the level of exposure of the body to these pollutants.The estimated daily intake (EDI) of the analyzed metals was calculated from Equation (2), according to the mean concentration of each metal in each food and the respective consumption rates.The EDI for individual food, total EDI for groups of the studied fruits and vegetables and all food, and maximum tolerable daily intake (MTDI) of the studied metals from the consumption of fruits and vegetables [56] are shown in Table 6.The highest total daily intake for all fruits was found for Zn (2.54•10 −3 mg/day) and Cu (1.17•10 −3 mg/day), with the highest EDI for melon (8.37•10 −4 and 6.92•10 −4 mg/day, respectively), whilst among the vegetables, the total daily intake was the highest for Fe (1.68•10 −2 mg/day) and Zn (8.37•10 −3 mg/day), with the highest EDI for potatoes (8.21•10 −3 and 3.51•10 −3 mg/day, respectively).In turn, the exposure to Cd and Cr was characterized by the lowest EDI values considering both the fruit alone, the vegetables alone, and their total.The total daily intakes of individual metals from all studied food were much lower than the MTDI values and constituted below 0.1% of this value for Cd, Cr, Cu, and Zn, 0.2% for Ni, and 0.6% for Pb.

Non-Carcinogenic Risk
The health risks caused by consuming contaminated fruits and vegetables were assessed based on the target hazard quotient (THQ) and total target hazard quotient (TTHQ) values, calculated from Equations ( 3) and (4).It is assumed that if the THQ is greater than 1, the exposed population is likely to suffer from the harmful effects of consuming contaminated food and appropriate interventions should be taken to eliminate the risk, and if the THQ < 1, the exposed population is unlikely to experience obvious adverse effects [57].None of the tested fruits and vegetables had a THQ for single metals above the critical value of 1 and decreased (considering the highest values) from 0.834 to 0 in the following order: Pb > Cu > Zn = Fe > Ni > Cr > Cd (Figure 6A).The studied food can be considered potentially safe for health; however, attention should be paid to lettuce with a THQ value for Pb close to 1.
Regarding the TTHQ values for all the metals in individual food (Figure 6A), the following decreasing order was observed: potato (1.392) > lettuce (1.114) > tomato (1.013) > green pepper (0.985) > carrot (0.631) > melon (0.511) > watermelon (0.280) > grape (0.242).TTHQ values greater or close to 1 in potato, lettuce, tomato, and green pepper indicate a potential health risk when consumed continuously.If the consumption of only fruits is assumed (Figure 6B), the TTHQs of all studied metals are low (below 0.5), proving that the consumption of grapes, melons, and watermelons is relatively safe for human health.In turn, if the consumption of only vegetables is assumed, the TTHQ values of Pb and Cu exceed 1 (2.458 and 1.221, respectively), suggesting health risks to consumers from the contaminated vegetables.The overall potential non-carcinogenic effect of all elements and food simultaneously, according to Equation (5), is expressed by a hazard index (HI).Regarding the consumption of all studied fruits, the HI was 1.068, while for all studied vegetables, it was 4.471, which gave a combined HI of 6.524 for the consumption of all considered food.An HI in the range between 1 and 10 indicates that consumers may experience adverse health effects following the ingestion of the food [58], less from fruits than from vegetables.Regarding the TTHQ values for all the metals in individual food (Figure 6A), the lowing decreasing order was observed: potato (1.392) > lettuce (1.114) > tomato (1.01 green pepper (0.985) > carrot (0.631) > melon (0.511) > watermelon (0.280) > grape (0.2 TTHQ values greater or close to 1 in potato, lettuce, tomato, and green pepper indica potential health risk when consumed continuously.If the consumption of only frui assumed (Figure 6B), the TTHQs of all studied metals are low (below 0.5), proving the consumption of grapes, melons, and watermelons is relatively safe for human he In turn, if the consumption of only vegetables is assumed, the TTHQ values of Pb and exceed 1 (2.458 and 1.221, respectively), suggesting health risks to consumers from contaminated vegetables.The overall potential non-carcinogenic effect of all elements food simultaneously, according to Equation (5), is expressed by a hazard index (HI) garding the consumption of all studied fruits, the HI was 1.068, while for all studied etables, it was 4.471, which gave a combined HI of 6.524 for the consumption of all sidered food.An HI in the range between 1 and 10 indicates that consumers may exp ence adverse health effects following the ingestion of the food [58], less from fruits from vegetables.

Carcinogenic Risk
Among the studied heavy metals, cadmium and lead can cause both non-carc genic and carcinogenic effects, depending on the exposure dose.Since cadmium was i tified at a concentration below the detection limit, the carcinogenic risk (CR) for the s ied food was mainly derived from Pb intake.The CR for Pb was the highest in the ca carrot (7. ).These values are below the acceptable limit value es lished by the USEPA to be 10 −4 , indicating no carcinogenic risk from consuming this m in the studied fruits and vegetables.

Carcinogenic Risk
Among the studied heavy metals, cadmium and lead can cause both non-carcinogenic and carcinogenic effects, depending on the exposure dose.Since cadmium was identified at a concentration below the detection limit, the carcinogenic risk (CR) for the studied food was mainly derived from Pb intake.The CR for Pb was the highest in the case of carrot ).These values are below the acceptable limit value established by the USEPA to be 10 −4 , indicating no carcinogenic risk from consuming this metal in the studied fruits and vegetables.

The Study Area
The present study was carried out from March to April 2020 in peri-urban areas of Boumerdes, located in the north of Algeria (Latitude: 36.7667,Longitude: 3.46667 36 • 46 ′ 0 ′′ N, 3 • 28 ′ 0 ′′ E and alt 2 m).As shown in Figure 7, the sampling sites were located along different rivers adjacent to road traffic in the regions of Boudouaou, Corso, Naceria, and Dellys.
The climate in Boumerdes is semi-arid, and rain in this area is much more important in the winter than in the summer.Boumerdes has an average annual temperature of 17.5 • C, with the highest temperature (about 27 • C) in August and the lowest (about 10 • C) in January.Over the year, the average rainfall is 672 mm, with about 97 mm in November and only 2 mm in July.In turn, the relative humidity of the year is the lowest in July (54.13%) and the highest in January (75.13%).The geological substrate of this region is regarded as a bilayer consisting of a Quaternary cover of mostly a Pliocene clayey sand layer and compact marl layer, with the exception of areas along rivers banks where additional Quaternary alluvium is present [59].On the farms where the samples were taken, groundwater (in Boudouaou) or river water (in Corso, Naceria, and Dellys) is used to irrigate the crops.There are several industrial activities located in the region under study (Figure 7), e.g., paper production (a), pharmaceuticals (b), plastic packaging (c), food (e), engineering and procurement (d), and chemical wholesale (f).
and only 2 mm in July.In turn, the relative humidity of the year is the lowest in July (54.13%) and the highest in January (75.13%).The geological substrate of this region is regarded as a bilayer consisting of a Quaternary cover of mostly a Pliocene clayey sand layer and compact marl layer, with the exception of areas along rivers banks where additional Quaternary alluvium is present [59].On the farms where the samples were taken, groundwater (in Boudouaou) or river water (in Corso, Naceria, and Dellys) is used to irrigate the crops.There are several industrial activities located in the region under study (Figure 7), e.g., paper production (a), pharmaceuticals (b), plastic packaging (c), food (e), engineering and procurement (d), and chemical wholesale (f).

Sample Preparation
Samples of water, soil, fruits, and vegetables collected from the selected farms were transported to the laboratory and properly prepared for the heavy metal analysis by the spectrometric method.

Water Samples
Water samples (9 from each sampling site) were collected from the canals and river in 250 mL plastic bottles, as a mixture of two independent subsamples.After transporting to the laboratory, they were centrifuged to remove the suspended particles, filtered through a 0.45 µm PTFE membrane filter (Omnipore, Merck KGaA, Darmstadt, Germany), preserved with 1.0 mL of 70% HNO 3 , and then stored in a refrigerator to reduce volatilization and biodegradation between sampling and analysis.

Soil Samples
Soil samples (9 from each sampling site) were collected randomly from agricultural plots using a stainless-steel auger at 0-30 cm depths and stored in plastic bags.From each plot, a soil sample of about 1 kg in weight consisting of a mixture of five subsamples collected from different sites of the studied area was taken.All samples were well mixed and riffled, and one-fourth of each sample was dried in the oven (Binder, Tuttlingen, Germany, model E028-230V-T) at 105 • C for 12 h.The dried samples were then ground and sieved with a 75 µm mesh size sieve and digested according to the method adopted by FAO/SIDA [60].The soil pH was measured by a pH meter (Hanna Instruments HI 2211, Woonsocket, RI, USA) using a soil-water ratio of 1:1 (USEPA method 9045D, Washington, DC, USA).
A sample of 20 g of pulverized (75 µm) soil was weighed in a 400 mL tall-form beaker, and a mixture of 50 mL HCl and 20 mL HNO 3 was slowly added to the sample.The contents of the beaker were continuously stirred with a glass rod to ensure that the soil sample was properly wetted and heated on a hot plate at 160 • C for a minimum of 45 min (until almost completely dry).Then, the sample was cooled, diluted in a 200 mL volumetric flask with deionized water, shaken and poured back into the beaker, and settled for 30 min.Finally, the sample was filtered and analyzed in 16 × 150 mm test tubes.

Fruit and Vegetable Samples
Samples of fruits (30 from each of the 3 types), including Vitis vinifera (grape), Cucumis melo var.saccharimus (melon), and Citrullus vulgaris.Schrade (watermelon), and vegetables (30 from each of the 6 types), including Lycopersicum esculentum L. (tomato), Cucurbita pepo (zucchini), Daucus carota (carrot), Lactuca sativa (lettuce), Convolvulus Batatas (potato), and Capsicum annuum L. (green pepper), were collected from the study agricultural plots at different times depending on their availability.The samples were transported to the laboratory in paper bags, cleaned with deionized water to remove dust and extraneous matter, and dried in the oven at 105 • C for 12 h.In the case of plants with typically inedible peels, such as melon, watermelon, potato, and carrot, they were peeled before drying.The dried samples were then ground, homogenized, and stored in tightly closed, clean sample bottles until digestion.A sample of 2 g of fruit/vegetable (see Table S1) was weighed in a test tube and digested on a hot plate in three 30 min steps with (1) 10.0 mL of 30% H 2 O 2 , (2) 10.0 mL of 70% HNO 3 , and (3) 10.0 mL of HClO 4 .Then, the sample was cooled, carefully transferred into a 50 mL volumetric flask, rinsed, and diluted with deionized water.The prepared samples were placed in 16 × 150 mm test tubes and analyzed.

Chemicals
All chemical reagents used for the sample preparation were of analytical grade and purchased from MERCK Chemicals (Darmstadt, Germany).Quality control of the analytical step was carried out using reagent blanks (HNO 3 /H 2 SO 4 /HClO 4 ratio 5:1:1) and NIST Standard Reference Materials (SRMs) 1643E (for water), 2709 (for soil), and 1570A (for vegetables).The relative standard deviation (RSD < 5%) was inspected by a tuning solution purchased from Thermo Fisher Scientific (Waltham, MA, USA).All samples and standards were diluted with deionized water.

Spectrometric Analysis
A flame atomic absorption spectrometer, Thermo Fisher Scientific ICE 3000 FAA (Waltham, MA, USA), fully automated and PC-controlled using SOLAAR Software 11.2 versions and equipped with a fast sequential operation for multi-element flame determinations, was used for Cd, Cr, Cu, Fe, Ni, Pb, and Zn analysis.The selected validation parameters of the applied method are shown in Table 7.

Calculations
For all calculations, the mean values of heavy metal concentrations in the water, soils, and food determined based on triplicate analysis of three independent samples were applied.
The transfer factor (TF) expressing the bioavailability of a metal at a particular position on a species of plant [61] was calculated based on the total metal content of the whole plant (edible part) without considering the various parts of the plant, using Equation (1) [51,52]: where C food -the mean metal concentration in fruit/vegetable (mg/kg); C soil -the mean metal concentration in soil (mg/kg).The estimated daily intakes (EDIs) of the studied metals were calculated using Equation ( 2), taking into account the mean concentration of individual metals in food samples, the weight of fruits and vegetables consumed by an individual, and the average weight of an adult in Boumerdes equal to 63.6 kg: where FIR-the food ingestion rate (g/day/person); CF-the conversion factor equal to 0.085 (for fruits and vegetables); and BW-the body weight [62].
For the determination of the TTHQ, indicating the risk of exposing a person to all metals simultaneously, the sum of the THQ obtained for individual metals was applied [62]: TTHQ(individual food) = THQ(metal 1) + THQ(metal 2) + . . .+ THQ(metal n) (4) In order to assess the overall potential for non-carcinogenic effects from more than one food, a hazard index (HI) was formulated based on the USEPA guidelines for health risk assessment of chemical mixtures [64], as follows: HI = n ∑ i=1 TTHQ i = TTHQ(food 1) + TTHQ(food 2) + . . .+ TTHQ(food n) (5) The carcinogenic risk can be expressed by the target cancer risk factor (TCR) determining the lifetime cancer risk, which can be calculated as follows [65]: where CSF o -the cancer slope factor attributed to the carcinogenic metal equal to 6.3 and 8.5•10 −3 (mg/kg/day) −1 for Cd and Pb [66,67], respectively; ATc-the average time for carcinogens.

Statistical Analysis
Statistica ver.13.3 software (Tibco Software Inc., Palo Alto, CA, USA) was used for the statistical treatment of the data.The results of the metal concentration analysis and pH measurements are expressed as mean values ± standard deviation (SD).Specific relationships between the individual metal concentrations and pH values were determined by the Pearson correlation.The cluster analysis (CA) was applied to visualize the relationship between the measured parameters, the sampling site or the type of food cultivated.Analyses were performed on standardized data.

Conclusions
This study demonstrates that the concentrations of cadmium (Cd), copper (Cu), chromium (Cr), iron (Fe), nickel (Ni), lead (Pb), and zinc (Zn) in both the soils and irrigation waters of Boumerdes city are below the maximum permissible levels set by the FAO/WHO.The highest transfer factors (TFs) were observed for Cr in carrots, Cu in tomatoes, and Fe, Ni, Pb, and Zn in lettuce.The potential non-carcinogenic health risks, as assessed through the estimated daily intake (EDI) and target hazard quotient (THQ) values for individual heavy metals, are considered low.Similarly, the carcinogenic risk (CR) from consuming fruits and vegetables containing Pb is minimal.However, the total noncarcinogenic risk (TTHQ) associated with the consumption of potatoes, lettuce, tomatoes, and green peppers is near or exceeds a value of 1, indicating potential health impacts.
In conclusion, while heavy metal levels are within safety limits, this study underscores the necessity for the ongoing monitoring of heavy metal concentrations in both agricultural soils and crops.Ensuring crop safety requires the effective management of agricultural practices, such as avoiding the use of untreated wastewater or excessive artificial fertilizers and protecting the surrounding environment from industrial pollution.

Figure 1 .
Figure 1.Comparison of the metal content in the waters of different regions of Algeria [26-32].

Figure 1 .
Figure 1.Comparison of the metal content in the waters of different regions of Algeria [26-32].

Figure 2 .
Figure 2. Dendrogram resulting from the hierarchical cluster analysis of the heavy metal concentrations in the studied soils.

Figure 2 .
Figure 2. Dendrogram resulting from the hierarchical cluster analysis of the heavy metal concentrations in the studied soils.

Figure 4 .
Figure 4. Dendrogram resulting from the hierarchical cluster analysis of the heavy metal concentration in the studied food.

Figure 4 .
Figure 4. Dendrogram resulting from the hierarchical cluster analysis of the heavy metal concentration in the studied food.

Figure 5 .
Figure 5. Transfer factors of the fruits and vegetables grown in the studied region of Boumerdes.

Figure 5 .
Figure 5. Transfer factors of the fruits and vegetables grown in the studied region of Boumerdes.

Figure 6 .
Figure 6.Target hazard quotient (THQ) (A) and total target hazard quotient (TTHQ) (B) for sumers of the food from the studied area.

Figure 6 .
Figure 6.Target hazard quotient (THQ) (A) and total target hazard quotient (TTHQ) (B) for consumers of the food from the studied area.

Table 1 .
The mean concentration of heavy metals in the irrigation water for crop production (SD: standard deviation; <LOD: below detection limit).

Table 2 .
pH and mean concentration of heavy metals in the studied soils (SD: standard deviation; <LOD: below detection limit).

Table 3 .
Pearson correlation coefficients of the heavy metal pollutants in the studied soils (significant values are bolded; * p < 0.05; ** p < 0.01).

Table 4 .
The mean concentration of heavy metals in the studied fruits and vegetables (SD: standard deviation; <LOD: below detection limit; n.a.: not available).

Table 4 .
The mean concentration of heavy metals in the studied fruits and vegetables (SD: standard deviation; <LOD: below detection limit; n.a.: not available).

Table 7 .
Selected validation parameters of the method used.