Highly Efficient Asymmetric [3+2] Cycloaddition Promoted by Chiral Aziridine-Functionalized Organophosphorus Compounds

The asymmetric [3+2] cycloaddition of azomethine ylides generated from the corresponding imino ester-to-trans-β-nitrostyrene catalysis by chiral aziridine-containing phosphines and phosphine oxides is described. Of the sixteen stereoisomers that could be formed as a result of the title reaction, three were formed, two of which were obtained in an enantiomerically enriched or pure form, and one in a racemic form. One of the products underwent epimerization under basic reaction conditions.


Introduction
Asymmetric synthesis including organocatalysis is still one of the most important and intensively researched methodologies for creating new carbon-carbon bonds [1].Within the above methodology, new trends are emerging, including, for example, photocatalysis [2] and asymmetric synthesis using free radicals [3].The latter can be used in the synthesis of chiral amines and their derivatives [4], as well as in reactions of the enantioselective formation of carbon-carbon bonds, e.g., the alpha-oxyamination of aldehydes [5].Among the very wide variety of asymmetric reactions, stereodifferentiating pericyclic reactions [6], including cycloadditions [7], deserve a special mention.Among the products of asymmetric cycloaddition reactions, chiral systems containing a pyrrolidine ring often play a key role in biological and pharmacological research [8].They may have a wide spectrum of activities-antibacterial, cytotoxic, antifungal, etc. [9].Some examples of substances containing a pyrrolidine motif available on the pharmaceutical market are Telaprevir, which is an antiviral agent used in the treatment of chronic hepatitis C virus infection [10]; Ombitasvir is also used as a strong inhibitor of SARS-CoV-2 [10]; Captopril is commonly used in the treatment of hypertension; and Clindamycin and Anisomycin have antibacterial properties [11] (Figure 1).
Based on our experience in the field of asymmetric synthesis using heterorganic ligands and organocatalysts [12], and taking into account the significant importance of chiral pyrrolidine systems in several areas of life and science [10,11], we decided to carry out the asymmetric [3+2] cycloaddition of azomethine ylides to nitrostyrene [8] using chiral, optically pure organophosphorus derivatives of aziridines as catalysts, namely phosphines [13], phosphine oxides [14], and aziridine-containing imines [15,16].The purpose of this study was also to expand the scope of applicability of the chiral catalysts we had previously obtained.It should also be emphasized that the asymmetric [3+2] cycloaddition reaction catalyzed by copper (II) complexes is a chemical transformation that has been quite significantly exploited in the literature [17,18].reaction catalyzed by copper (II) complexes is a chemical transformation that has been quite significantly exploited in the literature [17,18].

Asymmetric [3+2] Cycloaddition Reactions
All the aziridine derivatives 1-12 were examined for catalytic activity in the asymmetric [3+2] cycloaddition reaction occurring between trans--nitrostyrene and ethyl imino ester 13 (Scheme 1).These reactions were catalyzed using an in situ generated catalytic system consisting of copper triflate, a chiral ligand, and DBU as a basic additive.After the appropriate purification of crude mixtures by column chromatography, three diastereomeric products 16-18 were obtained.Two of them were identified based on the literature data as products exo 17a and endo 18a (Scheme 1) [21,22].However, compound 16a has not been described in the literature.The effectiveness of the ligands was determined based on the analysis of the optical purity of the obtained products using the HPLC method using a column with chiral support.The results are summarized in Table 2.

Asymmetric [3+2] Cycloaddition Reactions
All the aziridine derivatives 1-12 were examined for catalytic activity in the asymmetric [3+2] cycloaddition reaction occurring between trans-β-nitrostyrene and ethyl imino ester 13 (Scheme 1).These reactions were catalyzed using an in situ generated catalytic system consisting of copper triflate, a chiral ligand, and DBU as a basic additive.After the appropriate purification of crude mixtures by column chromatography, three diastereomeric products 16-18 were obtained.Two of them were identified based on the literature data as products exo 17a and endo 18a (Scheme 1) [21,22].However, compound 16a has not been described in the literature.The effectiveness of the ligands was determined based on the analysis of the optical purity of the obtained products using the HPLC method using a column with chiral support.The results are summarized in Table 2. Secondly, the corresponding imino esters 13-15 (Figure 3) being the substrates used for the in situ generation of azomethine ylides were prepared with the appropriate glycine esters and benzaldehyde in the presence of triethylamine according to the literature's general protocol [20].

Asymmetric [3+2] Cycloaddition Reactions
All the aziridine derivatives 1-12 were examined for catalytic activity in the asymmetric [3+2] cycloaddition reaction occurring between trans--nitrostyrene and ethyl imino ester 13 (Scheme 1).These reactions were catalyzed using an in situ generated catalytic system consisting of copper triflate, a chiral ligand, and DBU as a basic additive.After the appropriate purification of crude mixtures by column chromatography, three diastereomeric products 16-18 were obtained.Two of them were identified based on the literature data as products exo 17a and endo 18a (Scheme 1) [21,22].However, compound 16a has not been described in the literature.The effectiveness of the ligands was determined based on the analysis of the optical purity of the obtained products using the HPLC method using a column with chiral support.The results are summarized in Table 2.The analysis of the results showed that the use of structurally similar aziridine ands led to similar outcomes.The highest chemical yield of asymmetric [3+2] cycloa tion, up to 71%, was achieved using ligands with an imine group; however, the prod were formed without significant diastereoselectivity.The aziridine phosphine ligand sulted in the formation of diastereomeric products in a similar ratio, with only aziri phosphine ligand 2 shifting the equilibrium towards the formation of product exo with additional excellent enantioselectivity (up to 98% ee).In all the cases, products and 17a were formed in enantiomerically enriched forms, while compound 18a alw formed racemic mixtures.Unexpectedly, the use of ligand 9 containing an NH-aziri group led to the formation of racemic product endo 18a predominating over products and 17a.
In the next stage, it was decided to conduct an asymmetric [3+2] cycloaddition r tion, but instead of ethyl imino ester 13, methyl imino ester 14 was used.The second strate and the other reaction conditions remained unchanged (Scheme 2) (Table 3).Isopropyl aziridine phosphine oxide 6 was used as the ligand.In this reaction, two stereomeric products were acquired and also identified based on the literature-descr 1 H-NMR spectra of the exo 17b product [21], which was formed with a 70% enantiom excess.Based on the 1 H-NMR spectrum, the second of the formed diastereomers was identified as the 4-epi-endo 16b product [8].However, the third endo product formed in previous reaction with ethyl imino ester 13 was not obtained this time.The analysis of the results showed that the use of structurally similar aziridine ands led to similar outcomes.The highest chemical yield of asymmetric [3+2] cycloa tion, up to 71%, was achieved using ligands with an imine group; however, the prod were formed without significant diastereoselectivity.The aziridine phosphine ligand sulted in the formation of diastereomeric products in a similar ratio, with only aziri phosphine ligand 2 shifting the equilibrium towards the formation of product exo with additional excellent enantioselectivity (up to 98% ee).In all the cases, products and 17a were formed in enantiomerically enriched forms, while compound 18a alw formed racemic mixtures.Unexpectedly, the use of ligand 9 containing an NH-aziri group led to the formation of racemic product endo 18a predominating over products and 17a.
In the next stage, it was decided to conduct an asymmetric [3+2] cycloaddition r tion, but instead of ethyl imino ester 13, methyl imino ester 14 was used.The second strate and the other reaction conditions remained unchanged (Scheme 2) (Table 3).Isopropyl aziridine phosphine oxide 6 was used as the ligand.In this reaction, two stereomeric products were acquired and also identified based on the literature-descr 1 H-NMR spectra of the exo 17b product [21], which was formed with a 70% enantiom excess.Based on the 1 H-NMR spectrum, the second of the formed diastereomers was identified as the 4-epi-endo 16b product [8].However, the third endo product formed in The analysis of the results showed that the use of structurally similar aziridine ands led to similar outcomes.The highest chemical yield of asymmetric [3+2] cycloa tion, up to 71%, was achieved using ligands with an imine group; however, the prod were formed without significant diastereoselectivity.The aziridine phosphine ligand sulted in the formation of diastereomeric products in a similar ratio, with only aziri phosphine ligand 2 shifting the equilibrium towards the formation of product exo with additional excellent enantioselectivity (up to 98% ee).In all the cases, products and 17a were formed in enantiomerically enriched forms, while compound 18a alw formed racemic mixtures.Unexpectedly, the use of ligand 9 containing an NH-aziri group led to the formation of racemic product endo 18a predominating over products and 17a.
In the next stage, it was decided to conduct an asymmetric [3+2] cycloaddition r tion, but instead of ethyl imino ester 13, methyl imino ester 14 was used.The second strate and the other reaction conditions remained unchanged (Scheme 2) (Table 3).Isopropyl aziridine phosphine oxide 6 was used as the ligand.In this reaction, two stereomeric products were acquired and also identified based on the literature-descr 1 H-NMR spectra of the exo 17b product [21], which was formed with a 70% enantiom excess.Based on the 1 H-NMR spectrum, the second of the formed diastereomers was identified as the 4-epi-endo 16b product [8].However, the third endo product formed in The analysis of the results showed that the use of structurally similar aziridine ands led to similar outcomes.The highest chemical yield of asymmetric [3+2] cycloa tion, up to 71%, was achieved using ligands with an imine group; however, the prod were formed without significant diastereoselectivity.The aziridine phosphine ligand sulted in the formation of diastereomeric products in a similar ratio, with only aziri phosphine ligand 2 shifting the equilibrium towards the formation of product exo with additional excellent enantioselectivity (up to 98% ee).In all the cases, products and 17a were formed in enantiomerically enriched forms, while compound 18a alw formed racemic mixtures.Unexpectedly, the use of ligand 9 containing an NH-aziri group led to the formation of racemic product endo 18a predominating over products and 17a.
In the next stage, it was decided to conduct an asymmetric [3+2] cycloaddition tion, but instead of ethyl imino ester 13, methyl imino ester 14 was used.The second strate and the other reaction conditions remained unchanged (Scheme 2) (Table 3) Isopropyl aziridine phosphine oxide 6 was used as the ligand.In this reaction, two stereomeric products were acquired and also identified based on the literature-descr 1 H-NMR spectra of the exo 17b product [21], which was formed with a 70% enantiom excess.Based on the 1 H-NMR spectrum, the second of the formed diastereomers was The analysis of the results showed that the use of structurally similar aziridine ligands led to similar outcomes.The highest chemical yield of asymmetric [3+2] cycloaddition, up to 71%, was achieved using ligands with an imine group; however, the products were formed without significant diastereoselectivity.The aziridine phosphine ligands resulted in the formation of diastereomeric products in a similar ratio, with only aziridine phosphine ligand 2 shifting the equilibrium towards the formation of product exo 17a, with additional excellent enantioselectivity (up to 98% ee).In all the cases, products 16a and 17a were formed in enantiomerically enriched forms, while compound 18a always formed racemic mixtures.Unexpectedly, the use of ligand 9 containing an NH-aziridine group led to the formation of racemic product endo 18a predominating over products 16a and 17a.
In the next stage, it was decided to conduct an asymmetric [3+2] cycloaddition reaction, but instead of ethyl imino ester 13, methyl imino ester 14 was used.The second substrate and the other reaction conditions remained unchanged (Scheme 2) (Table 3).(S)-Isopropyl aziridine phosphine oxide 6 was used as the ligand.In this reaction, two diastereomeric products were acquired and also identified based on the literature-described 1 H-NMR spectra of the exo 17b product [21], which was formed with a 70% enantiomeric excess.Based on the 1 H-NMR spectrum, the second of the formed diastereomers was also identified as the 4-epi-endo 16b product [8].However, the third endo product formed in the previous reaction with ethyl imino ester 13 was not obtained this time.In the next approach, the reaction was carried out using tert-butyl ester 15 as the substrate (Scheme 3).In this reaction, three diastereomeric products 16-18 were obtained again, and their configuration was determined based on the literature data as 4-epi-endo 16c, exo 17c, and endo 18c [8].Interestingly, in this reaction, product 16c was formed in a small racemic amount (Table 4).In the next approach, the reaction was carried out using tert-butyl ester 15 as the substrate (Scheme 3).In this reaction, three diastereomeric products 16-18 were obtained again, and their configuration was determined based on the literature data as 4-epi-endo 16c, exo 17c, and endo 18c [8].Interestingly, in this reaction, product 16c was formed in a small racemic amount (Table 4).In the next approach, the reaction was carried out using tert-butyl ester 15 as the substrate (Scheme 3).In this reaction, three diastereomeric products 16-18 were obtained again, and their configuration was determined based on the literature data as 4-epi-endo 16c, exo 17c, and endo 18c [8].Interestingly, in this reaction, product 16c was formed in a small racemic amount (Table 4).In summary, after identifying all three products, it was concluded that the r proceeded according to a concerted mechanism, resulting initially in the formation products with exo 17 and endo 18 configurations.The formation of an additional d omeric product 16 should be impossible when trans-β-nitrostyrene is used as a su Therefore, based on the literature reports, it is believed that product 18 undergoes e ization under basic conditions (the aziridine ring exhibits a basic character).Epimer involves a change in the configuration of a substituent at a single stereogenic cen is a process described in the literature [8] for both methyl and tert-butyl imino este involving triethylamine).It is assumed that the aziridine ligands act as chiral base ing the selective epimerization of product 18 to product 16.Methyl derivatives In the next approach, the reaction was carried out using tert-butyl ester 15 as the substrate (Scheme 3).In this reaction, three diastereomeric products 16-18 were obtained again, and their configuration was determined based on the literature data as 4-epi-endo 16c, exo 17c, and endo 18c [8].Interestingly, in this reaction, product 16c was formed in a small racemic amount (Table 4).In summary, after identifying all three products, it was concluded that the reaction proceeded according to a concerted mechanism, resulting initially in the formation of two products with exo 17 and endo 18 configurations.The formation of an additional diastereomeric product 16 should be impossible when trans-β-nitrostyrene is used as a substrate.Therefore, based on the literature reports, it is believed that product 18 undergoes epimerization under basic conditions (the aziridine ring exhibits a basic character).Epimerization involves a change in the configuration of a substituent at a single stereogenic center and is a process described in the literature [8] for both methyl and tert-butyl imino esters (e.g., involving triethylamine).It is assumed that the aziridine ligands act as chiral bases, causing the selective epimerization of product 18 to product 16.Methyl derivatives are the most susceptible to this change; no endo product was observed because the entirety underwent epimerization.However, tert-butyl derivatives are the most resistant to epimerization; only trace amounts of product 16c were observed.The differences in the quantity of product formed during the epimerization process may result from the steric hindrance present in the individual compounds.
To confirm the epimerization process, an additional experiment was conducted involving the reaction of pure product 18a with the in situ generated catalytic system consisting of aziridine chiral ligand 11a, a copper triflate, and DBU (Scheme 4).The reaction was conducted under analogous conditions to the cycloaddition reaction.This test confirmed that the mixture contained the 4-epi-endo product 16a along with the initial endo compound 18a in a ratio of 0.6:1.0,demonstrating that the formation of product 16a occurred under the influence of the utilized catalytic system and confirming the previously assumed theory of epimerization.
Finally, we attempted to explain the stereochemical course of the titular reaction by proposing a transition state model (Figure 4).It follows that coordination through the oxygen and nitrogen atoms of the imino ester and the phosphorus and nitrogen atoms of the chiral ligand makes β-nitrostyrene approachable from both the endo and exo sides.The (S)-isopropyl moiety on aziridine causes there to be less space around the Cu atom, which makes the system more strained, and thus causes higher enantioselectivity in comparison with that of the (R)-isopropyl substituent.Moreover, the absolute configuration of the resulting products does not change due to changes in the ligand configuration because the resulting intermediate complex is quite rigid and cannot change the configuration during the approach of nitrostyrene.During the formation of the intermediate complex, it is possible to arrange the substituents in such a way that the ethoxy group of the imino ester is very closely oriented to the isopropyl group, which may cause additional interactions in such an energetically favorable conformation, and therefore the enantioselectivity is higher for ethyl esters.Additionally, when it comes to the phenomenon of epimerization, in the space, the NO 2 group is positioned further away from the ester group due to repulsive electrostatic interactions; therefore, the exo and 4-epi-endo products are preferred.derwent epimerization.However, tert-butyl derivatives are the most resistant to epimerization; only trace amounts of product 16c were observed.The differences in the quantity of product formed during the epimerization process may result from the steric hindrance present in the individual compounds.
To confirm the epimerization process, an additional experiment was conducted involving the reaction of pure product 18a with the in situ generated catalytic system consisting of aziridine chiral ligand 11a, a copper triflate, and DBU (Scheme 4).The reaction was conducted under analogous conditions to the cycloaddition reaction.This test confirmed that the mixture contained the 4-epi-endo product 16a along with the initial endo compound 18a in a ratio of 0.6:1.0,demonstrating that the formation of product 16a occurred under the influence of the utilized catalytic system and confirming the previously assumed theory of epimerization.Finally, we attempted to explain the stereochemical course of the titular reaction by proposing a transition state model (Figure 4).It follows that coordination through the oxygen and nitrogen atoms of the imino ester and the phosphorus and nitrogen atoms of the chiral ligand makes β-nitrostyrene approachable from both the endo and exo sides.The (S)isopropyl moiety on aziridine causes there to be less space around the Cu atom, which makes the system more strained, and thus causes higher enantioselectivity in comparison with that of the (R)-isopropyl substituent.Moreover, the absolute configuration of the resulting products does not change due to changes in the ligand configuration because the resulting intermediate complex is quite rigid and cannot change the configuration during the approach of nitrostyrene.During the formation of the intermediate complex, it is possible to arrange the substituents in such a way that the ethoxy group of the imino ester is very closely oriented to the isopropyl group, which may cause additional interactions in such an energetically favorable conformation, and therefore the enantioselectivity is higher for ethyl esters.Additionally, when it comes to the phenomenon of epimerization, in the space, the NO2 group is positioned further away from the ester group due to repulsive electrostatic interactions; therefore, the exo and 4-epi-endo products are preferred.

General Information
All reagents were used as obtained from commercial suppliers unless otherwise noted.The corresponding chiral catalysts 1-12, exactly, aziridine phosphines [13], aziridine phosphine oxides [14], phosphine oxide containing NH-aziridine subunit [19], and aziridine-containing imines [15,16], were prepared according to a literature report.Also, imino esters 13-15, being substrates for in situ generation of azomethine ylides, were obtained using a general protocol [20].NMR spectra for the solutions in deuterated chloroform (CDCl3) were recorded at 600 MHz ( 1 H NMR) and 150 MHz ( 13 C NMR) with a Bruker Avance III spectrometer using the solvent as an internal standard.The following abbreviations were used to describe the NMR spectra: δ, chemical shift (ppm); J, coupling constants (Hz); s, singlet; br.s, broad singlet; d, doublet; dd, double-doublet; t, triplet; q, quartet; and m, multiplet.Column chromatography was performed with silica gel using a solvent mixture of hexane/ethyl acetate as eluents (9:1).The enantiomeric excess (ee) values were determined by high-performance liquid chromatography (HPLC) with a chiral packed column (Chiralcel OD-H) using hexane and isopropanol as the mobile phase.

Asymmetric [3+2] Cycloaddition Reaction Catalyzed by Aziridine Derivatives 1-12-General Procedure
A copper triflate (CuOTf)2•C6H6 (0.1 mmol) and ligand (0.1 mmol) were placed in a flask, the whole mixture was cooled to 0 °C, and then DBU (12 μL) and anhydrous THF (4 mL) were added.The catalytic system was used for 4 hours at 0 °C.The mixture was cooled to −15 °C, and imino ester (0.5 mmol) was added and stirred for 10 min, after which trans-β-nitrostyrene (0.5 mmol) was added.The resulting mixture was stirred for 48 h at

General Information
All reagents were used as obtained from commercial suppliers unless otherwise noted.The corresponding chiral catalysts 1-12, exactly, aziridine phosphines [13], aziridine phosphine oxides [14], phosphine oxide containing NH-aziridine subunit [19], and aziridinecontaining imines [15,16], were prepared according to a literature report.Also, imino esters 13-15, being substrates for in situ generation of azomethine ylides, were obtained using a general protocol [20].NMR spectra for the solutions in deuterated chloroform (CDCl 3 ) were recorded at 600 MHz ( 1 H NMR) and 150 MHz ( 13 C NMR) with a Bruker Avance III spectrometer (Bruker, Billerica, MA, USA) using the solvent as an internal standard.The following abbreviations were used to describe the NMR spectra: δ, chemical shift (ppm); J, coupling constants (Hz); s, singlet; br.s, broad singlet; d, doublet; dd, double-doublet; t, triplet; q, quartet; and m, multiplet.Column chromatography was performed with silica gel using a solvent mixture of hexane/ethyl acetate as eluents (9:1).The enantiomeric excess (ee) values were determined by high-performance liquid chromatography (HPLC) with a chiral packed column (Chiralcel OD-H) using hexane and isopropanol as the mobile phase.

Asymmetric [3+2] Cycloaddition Reaction Catalyzed by Aziridine Derivatives 1-12-General Procedure
A copper triflate (CuOTf) 2 •C 6 H 6 (0.1 mmol) and ligand (0.1 mmol) were placed in a flask, the whole mixture was cooled to 0 • C, and then DBU (12 µL) and anhydrous THF (4 mL) were added.The catalytic system was used for 4 h at 0 • C. The mixture was cooled to −15 • C, and imino ester (0.5 mmol) was added and stirred for 10 min, after which trans-β-nitrostyrene (0.5 mmol) was added.The resulting mixture was stirred for 48 h at a low temperature, and then the solvent was evaporated in vacuo.The crude products were separated via column chromatography with silica gel (hexane-ethyl acetate 9:1).All the aziridine derivatives 1-12 were examined for catalytic activity in the asymmetric [3+2] cycloaddition of ethyl imino ester 13.In the asymmetric [3+2] cycloaddition of methyl imino ester 14 and tert-butyl imino ester 15, only catalyst 6 was examined.Copies of all the NMR spectra are included in Supplementary Materials.

Figure 1 .
Figure 1.Examples of substances containing a pyrrolidine motif.
Figure 1.Examples of substances containing a pyrrolidine motif.