Reaction-Based Amine and Alcohol Gases Detection with Triazine Ionic Liquid Materials

We demonstrated in this work the use of affinity ionic liquids, AIL 1 and AIL 2, for chemoselective detection of amine and alcohol gases on a quartz crystal microbalance (QCM). These detections of gaseous amines and alcohols were achieved by nucleophilic aromatic substitution reactions with the electrophilic 1,3,5-triazine-based AIL 1 thin-coated on quartz chips. Starting with inexpensive reagents, bicyclic imidazolium ionic liquids AIL 1 and AIL 2 were readily synthesized in six and four synthetic steps with high isolated yields: 51% and 63%, respectively. The QCM platform developed in this work is readily applicable and highly sensitive to low molecular weight amine gases: for isobutylamine gas (a bacterial volatile) at 10 Hz decrease in resonance frequency (i.e., ΔF = −10 Hz), the detectability using AIL 1 was 6.3 ppb. Our preliminary investigation on detection of the much less nucleophilic alcohol gas by AIL 1 was also promising. To our knowledge, no example to date of reports based on nucleophilic aromatic substitution reactions demonstrating sensitive gas detection in these triazine ionic liquids on a QCM has been reported.


Introduction
This work describes an ionic liquid system specifically developed for the detection of amine and alcohol gases by quartz crystal microbalance (QCM) in real-time and at ambient temperature. This label-free and chemoselective QCM detection of amine and alcohol gases was achieved by nucleophilic aromatic substitution (S N Ar) reactions with affinity ionic liquid AIL 1 thin-coated on quartz chips (Figure 1). The structure of AIL 1 is engineered with a super electrophilic 2,4-dichloro-1,3,5-triazine group responsible for capturing amine and alcohol vapor. Upon S N Ar reaction of AIL 1 with a nucleophilic amine or alcohol gas, a resonance frequency drop is readily detected by a mass-sensitive QCM.

Introduction
This work describes an ionic liquid system specifically developed for the detection of amine and alcohol gases by quartz crystal microbalance (QCM) in real-time and at ambient temperature. This label-free and chemoselective QCM detection of amine and alcohol gases was achieved by nucleophilic aromatic substitution (SNAr) reactions with affinity ionic liquid AIL 1 thin-coated on quartz chips (Figure 1). The structure of AIL 1 is engineered with a super electrophilic 2,4-dichloro-1,3,5-triazine group responsible for capturing amine and alcohol vapor. Upon SNAr reaction of AIL 1 with a nucleophilic amine or alcohol gas, a resonance frequency drop is readily detected by a masssensitive QCM. QCM has been in constant focus of research on chemical analysis in both solutions and gas phases over the years [1][2][3]. This is due primarily to its low cost and real-time analysis of adsorption QCM has been in constant focus of research on chemical analysis in both solutions and gas phases over the years [1][2][3]. This is due primarily to its low cost and real-time analysis of adsorption or Molecules 2020, 25, 104 2 of 11 recognition processes onto the surface of affinity materials such as ionic liquids deposited on electrodes when being operated at room temperature.
Ionic liquids are low-melting organic molten salts composed entirely of cations and anions [4,5]. Organic cations often are nitrogen-or phosphorus-containing compounds that are weakly associated with the anions, often resulting in liquidous salts at ambient temperature. Typically, one of the ions has a delocalized charge such that the formation of the stable crystal lattice is prevented and electrostatic interactions enfold both ions together. These liquids are green solvents because of their ability to dissolve a wide range of chemicals and their very low volatilities and thus negligible vapor pressures.
Cyanuric chloride (2,4,6-trichloro-1,3,5-triazine) and its derivatives are well known for their wide use in pesticide industry, organic synthesis [6,7], and peptide chemistry [8]. Although this class of compounds has been well recognized for their usefulness in chemical applications, its potential in chemoselective gas analysis remains unexplored. We envisioned that nonvolatile ionic liquids embedded with this dichlorotriazine group would be excellent for use in analysis of both amine and alcohol gases. Herein we are reporting our preliminary results on the study of QCM detections of both alcohol and amine gases with a 1,3,5-triazine-conjugated AIL 1 by S N Ar reactions.
The S N Ar reaction typically involves a nucleophilic addition to an arene in polar aprotic solvents followed by the elimination of a leaving group. For a smooth S N Ar reaction with stabilization of its anionic reaction intermediate (the Meisenheimer complex), it usually requires the presence of at least one electron-withdrawing substituent on the aromatic ring [9]. It is also reported in literature that S N Ar reactions proceed with faster reaction rates in ionic liquids than those performed in molecular solvents [10][11][12][13]. These rate accelerations were primarily attributed to both the enhanced nucleophilicity of the nucleophiles such as amines in ionic liquids and the favorable π + -π interactions between cations of ionic liquids and aromatic rings present on the substrates, rendering a more positive charge on the reacting ipso carbon [14]. Moreover, with comparison to the electrostatically neutral reactants, ionic liquids stabilize the charged transition states and also the intermediate Meisenheimer complexes formed [15]. Based specifically on S N Ar reactions, this work reports the synthesis of affinity ionic liquids AIL 1-2 and demonstrates their usefulness in amine and alcohol gases detection on QCM.

Results and Discussion
Scheme 1A illustrates the synthesis of AIL 1 of which the affinity element could be readily assembled from the commercially inexpensive cyanuric chloride (3), with a freshly prepared amine ionic liquid at ambient temperature. We commenced our synthesis of affinity ionic liquid AIL 1 from an inexpensive 2-(2-aminoethoxy)ethanol (1), which underwent a series of reactions (i.e., amine protection, alcohol mesylation, nucleophilic substitution with a bicyclic 6,7-dihydro-5H-pyrrolo[1,2-a]imidazole (2) previously developed in our laboratory [16], amine deprotection, and, lastly, anion methathesis with lithium bis(trifluoromethane)sulfonimide (LiNTf 2 ) in water) to afford first an amine-functionalized ionic liquid. This ionic liquid then reacted with cyanuric chloride (3) through a S N Ar reaction, as the last step in synthesis, to finally achieve the desired AIL 1. Using a different synthetic approach shown in Scheme 1B, the control ionic liquid AIL 2 was assembled first from the S N Ar reaction of the amine 1 with commercial 2-chloro-4,6-dimethoxy-1,3,5-triazine (4) under heated conditions followed by alcohol mesylation, nucleophilic substitution with 2, and lastly an anion metathesis to give AIL 2. The syntheses were straightforward and, in our hands, the overall isolated yields for these 6-and 4-step syntheses of AIL 1 and AIL 2 were high: 51% and 63%, respectively. Both AILs are viscous pale yellow liquid once obtained and become off-white solid if stored at room temperature for long hours (>1 day), which shows typical properties of supercooled fluids ( Figure S1) [17,18]. H-1 and C-13 NMR, and high-resolution mass spectrometry (HRMS) spectra of AIL 1 and 2 are summarized in the Supporting Information. Scheme 1. Synthesis of affinity ionic liquid AIL 1 (A) and its control ionic liquid AIL 2 (B). Boc2O, ditert-butyl dicarbonate; MsCl, methanesulfonyl chloride; TFA, trifluoroacetic acid.
Analysis and detection of volatile organic compounds (VOCs) are greatly important for a myriad of applications in health, safety, and environment. Moreover, continuous analysis of volatile biogenic amines is important for food spoilage detection (<300 ppb) [19][20][21]. To selectively detect gaseous amines and alcohols, in this work, we incorporated 2,4-dichloro-1,3,5-triazine group in AIL 1 for reasons that it is a super electrophile and has also been studied extensively in chemistry literature and showed its potential value in target gases detection on QCM.
When injected, amine gas is diffused into a flow QCM device, then dissolved and reacts chemoselectively with AIL 1, the frequency shift detected by a QCM is proportional to the mass change of ionic liquid film coated onto a quartz resonator. In principle, the faster the flow rate of the nitrogen carrier gas is, the lesser time an amine gas can react with AIL 1 and, accordingly, the lower the detectability shall result. For an empirical observation that the flow rate of 3 mL/min in our QCM measurements readily established stable baselines and experimentally produced reproducible sensorgrams, we decided to maintain this slow flow rate carrying a compromised long reaction time but without sacrificing detection sensitivity. Figure 2 displays the QCM results. It is clearly demonstrated that, using the same concentration (100 ppb) for all gaseous samples tested, AIL 1 only reacted chemoselectively with amine gas: ΔF = −175 Hz for isobutylamine [22]. This reaction-based AIL-on-QCM system worked well and was totally unreactive to common VOCs gases such as water, acetone, methanol, ethyl acetate, THF, and hexane (ΔF ~ 0 Hz), and, as a result, the frequency drop in this continuous flow measurement was not at all due to any nonspecific physisorption of gas onto ionic liquid. It is worth mentioning that, in our hand, this hydrophobic AIL-on-QCM platform developed was insensitive to moisture, indicating that any residual water present in samples and in the nitrogen gas stream would not interfere with amine gas analysis. As the control affinity ionic liquid, AIL 2 is inert to isobutylamine and all other gases tested (Figure 2), clearly showing the chemoselectivity of AIL 1 toward amine gases. An electrospray ionization-high-resolution mass spectrometry (ESI-HRMS) analysis supported the formation of the product of AIL 1 reaction with Scheme 1. Synthesis of affinity ionic liquid AIL 1 (A) and its control ionic liquid AIL 2 (B). Boc 2 O, di-tert-butyl dicarbonate; MsCl, methanesulfonyl chloride; TFA, trifluoroacetic acid.
Analysis and detection of volatile organic compounds (VOCs) are greatly important for a myriad of applications in health, safety, and environment. Moreover, continuous analysis of volatile biogenic amines is important for food spoilage detection (<300 ppb) [19][20][21]. To selectively detect gaseous amines and alcohols, in this work, we incorporated 2,4-dichloro-1,3,5-triazine group in AIL 1 for reasons that it is a super electrophile and has also been studied extensively in chemistry literature and showed its potential value in target gases detection on QCM.
When injected, amine gas is diffused into a flow QCM device, then dissolved and reacts chemoselectively with AIL 1, the frequency shift detected by a QCM is proportional to the mass change of ionic liquid film coated onto a quartz resonator. In principle, the faster the flow rate of the nitrogen carrier gas is, the lesser time an amine gas can react with AIL 1 and, accordingly, the lower the detectability shall result. For an empirical observation that the flow rate of 3 mL/min in our QCM measurements readily established stable baselines and experimentally produced reproducible sensorgrams, we decided to maintain this slow flow rate carrying a compromised long reaction time but without sacrificing detection sensitivity. Figure 2 displays the QCM results. It is clearly demonstrated that, using the same concentration (100 ppb) for all gaseous samples tested, AIL 1 only reacted chemoselectively with amine gas: ∆F = −175 Hz for isobutylamine [22]. This reaction-based AIL-on-QCM system worked well and was totally unreactive to common VOCs gases such as water, acetone, methanol, ethyl acetate, THF, and hexane (∆F~0 Hz), and, as a result, the frequency drop in this continuous flow measurement was not at all due to any nonspecific physisorption of gas onto ionic liquid. It is worth mentioning that, in our hand, this hydrophobic AIL-on-QCM platform developed was insensitive to moisture, indicating that any residual water present in samples and in the nitrogen gas stream would not interfere with amine gas analysis. As the control affinity ionic liquid, AIL 2 is inert to isobutylamine and all other gases tested (  Figure S2).

Figure 2.
Chemoselective detection of water, acetone, methanol, ethyl acetate, tetrahydrofuran (THF), hexane, and isobutylamine gases (100 ppb each), all by a 9 MHz quartz crystal microbalance (QCM) thin-coated with AIL 1 and AIL 2 (33 nmol each, 20.6 μg for AIL 1 and 20.3 μg for AIL 2, respectively; 300 nm thickness). The QCM sensorgrams for water, acetone, methanol, ethyl acetate, THF, and hexane gases were vertically shifted (15 Hz in between) for clarity. Sets of sensorgrams from gas reactions with AIL 1 and AIL 2 were grouped, vertically shifted, and separated (30 Hz in between AIL 1 and AIL 2). Nitrogen was used as the carrier gas with a flow rate of 3 mL/min, and gaseous samples were injected at 500 s. The resonance frequency drop, ΔF, in Hz is the QCM response on the quartz chip surface.
As the 2,4-dichloro-1,3,5-triazine-conjugated AIL 1 was successfully developed for sensitive analysis of amine gases, we next turned our attention to a detailed quantitative study of AIL 1 and AIL 2 reactions with isobutylamine gas based on SNAr reactions by QCM. Figure 4 provides the results. The AIL 1 showed an essentially linear QCM frequency response within the range of concentrations tested (0-300 ppb) (blue sensorgrams in Figure 4A). In our hand, AIL 1 is highly sensitive to amine gas: for isobutylamine gas at 10 Hz decrease in resonance frequency (i.e., ΔF = −10 Hz), the detectability using AIL 1 was 6.3 ppb (10/1.5874) (blue fitting line in Figure 4B). The QCM responses (ΔF) of the control AIL 2 reactions with isobutylamine gas (0-300 ppb) were completely negligible (red sensorgrams in Figure 4A and red fitting line in Figure 4B).

Figure 2.
Chemoselective detection of water, acetone, methanol, ethyl acetate, tetrahydrofuran (THF), hexane, and isobutylamine gases (100 ppb each), all by a 9 MHz quartz crystal microbalance (QCM) thin-coated with AIL 1 and AIL 2 (33 nmol each, 20.6 µg for AIL 1 and 20.3 µg for AIL 2, respectively; 300 nm thickness). The QCM sensorgrams for water, acetone, methanol, ethyl acetate, THF, and hexane gases were vertically shifted (15 Hz in between) for clarity. Sets of sensorgrams from gas reactions with AIL 1 and AIL 2 were grouped, vertically shifted, and separated (30 Hz in between AIL 1 and AIL 2). Nitrogen was used as the carrier gas with a flow rate of 3 mL/min, and gaseous samples were injected at 500 s. The resonance frequency drop, ∆F, in Hz is the QCM response on the quartz chip surface.
As the 2,4-dichloro-1,3,5-triazine-conjugated AIL 1 was successfully developed for sensitive analysis of amine gases, we next turned our attention to a detailed quantitative study of AIL 1 and AIL 2 reactions with isobutylamine gas based on S N Ar reactions by QCM. Figure 4 provides the results. The AIL 1 showed an essentially linear QCM frequency response within the range of concentrations tested (0-300 ppb) (blue sensorgrams in Figure 4A). In our hand, AIL 1 is highly sensitive to amine gas: for isobutylamine gas at 10 Hz decrease in resonance frequency (i.e., ∆F = −10 Hz), the detectability using AIL 1 was 6.3 ppb (10/1.5874) (blue fitting line in Figure 4B). The QCM responses (∆F) of the control AIL 2 reactions with isobutylamine gas (0-300 ppb) were completely negligible (red sensorgrams in Figure 4A and red fitting line in Figure 4B). MHz QCM thin-coated with AIL 1 and AIL 2 (33 nmol each, 300 nm thickness). Sets of sensorgrams from individual gas reactions with AIL 1 and AIL 2 were grouped and vertically shifted (15 Hz in between AIL 1 and AIL 2). All QCM sensorgrams for AIL 2 were vertically shifted (15 Hz in between) and separated for clarity. Nitrogen was used as the carrier gas with a flow rate of 3 mL/min, and gaseous samples were injected at 500 s. MHz QCM thin-coated with AIL 1 and AIL 2 (33 nmol each, 300 nm thickness). Sets of sensorgrams from individual gas reactions with AIL 1 and AIL 2 were grouped and vertically shifted (15 Hz in between AIL 1 and AIL 2). All QCM sensorgrams for AIL 2 were vertically shifted (15 Hz in between) and separated for clarity. Nitrogen was used as the carrier gas with a flow rate of 3 mL/min, and gaseous samples were injected at 500 s.  The aforementioned QCM results further prompted us to initiate a preliminary investigation of the detection of alcohol gases by AIL 1. Since alcohols are much less reactive than amines, we envisioned that Lewis acids should make AIL 1 become more electrophilic and facilitate their reactions. Pleasingly, encouraging results were obtained when Sc(OTf) 3 was used with AIL 1 in alcohol gas detection. Our preliminary result is given in Figure 5. Albeit AIL 1 detects low concentrations of ethanol gas (250 and 500 ppb), we found that 10 mol% Sc(OTf) 3 Lewis acid further promoted the reaction of AIL 1 with ethanol gas: at 250 ppb, ∆F = −52 Hz with Sc(OTf) 3 and −14 Hz with no Lewis acid; at 500 ppb, ∆F = −83 Hz with Sc(OTf) 3 and −20 Hz with no Lewis acid, respectively. Results in Figure 5 also unambiguously show that AIL 2 was inert to ethanol gas (∆F = 0 Hz at 250 and 500 ppb), further proving that the frequency drop in the continuous flow QCM measurement was not at all due to the nonspecific dissolution of ethanol gas in AIL 1. This preliminary result revealed that AIL 1 is chemoselective and AIL 2 is inert to ethanol gas (  Figure S4).
(33 nmol each, 300 nm thickness). Each data point was taken at t = 10,000 s from the resonance frequency drop value of various concentrations of isobutylamine gas. Linear curve fitting equation (determined by KaleidaGraph) for AIL 1: ΔF = −1.5874[isobutylamine] (R = 0.99825). Nitrogen was used as the carrier gas with a flow rate of 3 mL/min, and gaseous samples were injected at 500 s. The QCM sensorgrams for AIL 2 were vertically shifted (20 Hz in between) for clarity.
The aforementioned QCM results further prompted us to initiate a preliminary investigation of the detection of alcohol gases by AIL 1. Since alcohols are much less reactive than amines, we envisioned that Lewis acids should make AIL 1 become more electrophilic and facilitate their reactions. Pleasingly, encouraging results were obtained when Sc(OTf)3 was used with AIL 1 in alcohol gas detection. Our preliminary result is given in Figure 5. Albeit AIL 1 detects low concentrations of ethanol gas (250 and 500 ppb), we found that 10 mol% Sc(OTf)3 Lewis acid further promoted the reaction of AIL 1 with ethanol gas: at 250 ppb, ΔF = −52 Hz with Sc(OTf)3 and −14 Hz with no Lewis acid; at 500 ppb, ΔF = −83 Hz with Sc(OTf)3 and −20 Hz with no Lewis acid, respectively. Results in Figure 5 also unambiguously show that AIL 2 was inert to ethanol gas (ΔF = 0 Hz at 250 and 500 ppb), further proving that the frequency drop in the continuous flow QCM measurement was not at all due to the nonspecific dissolution of ethanol gas in AIL 1. This preliminary result revealed that AIL 1 is chemoselective and AIL 2 is inert to ethanol gas (

General Information
The 1 H-NMR and 13 C-NMR spectra were recorded at 400 MHz and 100 MHz, respectively, on a Bruker AVANCEIII HD 400 NMR spectrometer (Bruker BioSpin GmbH, Rheinstetten, Germany) in deuterated solvents (CDCl3, DMSO-d6 or CD3CN) using tetramethylsilane (TMS) as the internal standard. The chemical shift (δ) for 1 H and 13 C are given in ppm relative to the residual signal of the solvent. Coupling constants are given in Hz. The following abbreviations are used to indicate the multiplicity: s, singlet; d, doublet; t, triplet; q, quartet; quin, quintet; m, multiplet; dd, doublet of doublets; td, triplet of doublets; dt, doublet of triplets; ddd, doublet of doublet of doublets; bs, broad signal. The reactions were monitored using TLC (thin-layer chromatography) silica gel 60 F254 (Merck KGaA, Darmstadt, Germany). Evaporation of solvents was performed under reduced pressure. Melting points were measured and recorded by the OptiMelt MPA-100 apparatus (Standford Research Systems, Sunnyvale, CA, USA) and uncorrected. Figure 5. Detection of ethanol gas (250 and 500 ppb). All QCM sensorgrams were vertically shifted (30 Hz in between) and separated for clarity. Nitrogen was used as the carrier gas with a flow rate of 3 mL/min, and gaseous samples were injected at 1000 s.

General Information
The 1 H-NMR and 13 C-NMR spectra were recorded at 400 MHz and 100 MHz, respectively, on a Bruker AVANCEIII HD 400 NMR spectrometer (Bruker BioSpin GmbH, Rheinstetten, Germany) in deuterated solvents (CDCl 3 , DMSO-d 6 or CD 3 CN) using tetramethylsilane (TMS) as the internal standard. The chemical shift (δ) for 1 H and 13 C are given in ppm relative to the residual signal of the solvent. Coupling constants are given in Hz. The following abbreviations are used to indicate the multiplicity: s, singlet; d, doublet; t, triplet; q, quartet; quin, quintet; m, multiplet; dd, doublet of doublets; td, triplet of doublets; dt, doublet of triplets; ddd, doublet of doublet of doublets; bs, broad signal. The reactions were monitored using TLC (thin-layer chromatography) silica gel 60 F254 (Merck KGaA, Darmstadt, Germany). Evaporation of solvents was performed under reduced pressure. Melting points were measured and recorded by the OptiMelt MPA-100 apparatus (Standford Research Systems, Sunnyvale, CA, USA) and uncorrected.
In addition to cyanuric chloride, all starting materials, reagents, and solvents were purchased from commercial sources and used as such without further purification. Unless otherwise stated, all reactions were carried out under an inert atmosphere using anhydrous solvents.
The desired product in reaction mixture was purified by silica gel column chromatography, first, using eluents containing ethyl acetate/hexane = 1:1 to 1:0 (v/v), and then changing mobile phase to methanol to eventually afford the Boc-protected amine ionic liquid (0.903 g, 93% yield) as a colorless liquid.

Deprotection of Boc-Protected Amine Ionic Liquid
To a 20-mL sample bottle containing Boc-protected amine ionic liquid (824 mg, 2.11 mmol) in dichloromethane (9.7 mL), trifluoroacetic acid (0.8 mL, 137.52 mmol) was added for Boc-deprotection. The reaction progress was monitored by TLC and its reaction was carried out for 13.5 h at ambient temperature. The solvent and excess TFA was removed under reduced pressure and concentrated in vacuo for 5 h to afford a yellow waxy product without further purification.

Synthesis of Amine Ionic Liquid
To an ice chilled container containing the yellow waxy compound was first added 10 wt% NaHCO 3 (2.2 mL), followed by LiNTf 2 (1.222 g, 4.26 mmol). The solution mixture became two phases within 5 min in which the desired hydrophobic amine ionic liquid was in the bottom phase. The solution was stirred for a total of 14 h at ambient temperature. The bottom ionic liquid was collected, washed with water and dichloromethane, and concentrated in vacuo to finally afford the amine ionic liquid as To a round-bottomed flask containing cyanuric chloride 3 (178 mg, 0.97 mmol) and NaHCO 3 (69 mg, 0.82 mmol) in acetonitrile (3.0 mL) at 0 • C, a acetonitrile solution (5 mL) containing the amine ionic liquid (256 mg, 0.54 mmol) was added dropwise for a period of 30 min. The nucleophilic aromatic substitution reaction was carried out at ambient temperature for 4 h, determined by TLC.
Salts formed in the reaction mixture were filtered off. The solvent was removed under reduced pressure and concentrated in vacuo for 30 min. The crude product was mixed with dichloromethane (30 mL) and washed with 10 wt% citric acid (15 mL × 3). The organic layer collected was dried over anhydrous Na 2 SO 4 , filtered and concentrated in vacuo for 3 h. The desired product was thoroughly washed with n-hexane, followed by chloroform to remove excessive cyanuric chloride 3 and its hydrolysis impurities to finally afford the pure AIL 1 (219 mg, 65% yield) as ivory white solid.
The resulting reaction solution was further diluted with dichloromethane (14 mL), then sequentially extracted with 10 wt% citric acid (7 mL × 3) and 10 wt% NaHCO 3 (7 mL × 3). The organic layer collected was dried over anhydrous Na 2 SO 4 , filtered, concentrated under reduced pressure and in vacuo to afford a light yellow liquid product (563 mg, 97% yield).
The desired product in reaction mixture was purified by silica gel column chromatography, first, using eluents containing ethyl acetate/hexane = 1:1 to 1:0 (v/v), and then changing mobile phase to methanol to eventually afford the triazine ionic liquid (116 mg, 83% yield) as colorless liquid.

Synthesis of AIL 2
To a ice chilled solution of the triazine ionic liquid (112 mg, 0.26 mmol), LiNTf 2 (90 mg, 0.31 mmol) in water (0.4 mL) and dichloromethane (0.4 mL) was added The ion-exchange reaction was vigorously stirred for 13 h at ambient temperature. The upper aqueous layer was removed, and the bottom dichloromethane solution was extracted with water (1 mL × 4). The organic layer collected was dried over anhydrous Na 2 SO 4 , filtered, and concentrated under reduced pressure to eventually afford the desired AIL2 (158 mg, 98% yield) as white solid.

QCM Measurements
A flow PSS (Piezo Sensor System) QCM system (9 MHz) available from the ANT Technology Co. (Taipei, Taiwan) was operated at room temperature and nitrogen was used as carrier gas. The 9-MHz AT-cut quartz chips deposited with gold electrodes (area 11 mm 2 ) on both sides were also available from ANT Technology. Before use, the gold electrodes on chips were cleaned sequentially with 5 N NaOH (30 min), H 2 O (5 min), 1 N HCl (5 min), and H 2 O (5 min). This sequence of chip cleaning was repeated two times, followed by washing with MeOH (30 min), H 2 O (10 min), and H 2 O (10 min) to remove any organic absorbents, and finally rinsed with water thoroughly and dried under nitrogen.
Ionic liquid solutions were prepared by dissolving individual AIL (10 µmol) in acetonitrile (HPLC grade, 300 µL). The freshly prepared solutions (1 µL) were carefully pipetted onto the cleaned bare gold electrode situated at the center of quartz chips. The ionic liquid-coated chips were placed in an oven (120 • C) for 1 min to remove residual solvent. The quartz sensor chips were then mounted in the gas flow chamber (100 cm 3 ) and nitrogen was used as carrier gas at flow rate of 3 mL/min. Until a stable baseline was obtained, target gas samples were injected into the chamber. The resonance frequency drops versus time curves were measured and recorded. Typically, the used quartz chips could be readily regenerated by a simple wash with acetonitrile or methanol solvent.
Target sample vapors were obtained by gasifying the chemicals in the sealed glass container (1.25 L). Using the PSS QCM apparatus for QCM measurements, a rapid initial nonspecific frequency decrease (approximately 0.1 to 0.4 Hz) was typically detected within 5 s after sample injection, which was totally insignificant with reference to reaction-based frequency drops owing to nucleophilic aromatic substitution reactions of AILs with target gases.

Conclusions
We demonstrated in this work the use of affinity ionic liquids, AIL 1 and AIL 2, for chemoselective detection of amine [23] and alcohol [24] gases on a quartz crystal microbalance. These detections of gaseous amines and alcohols were achieved by S N Ar reactions with the super electrophilic 1,3,5-triazine-based AIL 1 thin-coated on quartz chips. Our work holds three important conclusions: (i) affinity ionic liquid AIL 1 developed in this work is highly sensitive to the chemoselective detection of amine gases, (ii) our QCM analysis of S N Ar reactions by AIL 1 is straightforward and label-free, and (iii) our ionic liquid platform is chemoselective, readily applicable to analysis of small molecular weight amine and alcohol gases. To the best of our knowledge, we know of no examples to date of reports based on S N Ar reactions demonstrating sensitive amine as well as alcohol gas detection with 1,3,5-triazine ionic liquids on a QCM.