Antihypertensive Effects of Corn Silk Extract and Its Novel Bioactive Constituent in Spontaneously Hypertensive Rats: The Involvement of Angiotensin-Converting Enzyme Inhibition

Corn silk tea has been used in folk medicine for anti-hypertensive healthcare. Angiotensin-converting enzyme (ACE) plays a crucial role on the homeostasis of blood pressure. However, effects of corn silk tea on ACE activity and the presence of ACE inhibitory constituents in corn silk are still unknown. Here we applied proteomics and bioinformatics approaches to identify corn silk bioactive peptides (CSBps) that target ACE from the boiling water extract of corn silk (CSE). CSE significantly reduced systolic blood pressure (SBP) levels in spontaneously hypertensive rats and inhibited the ACE activity. By proteomics coupled with bioinformatics analyses, we identified a novel ACE inhibitory peptide CSBp5 in CSE. CSBp5 significantly inhibited the ACE activity and decreased SBP levels in a dose-dependent manner. Docking analysis showed that CSBp5 occupied the substrate-binding channel of ACE and interacted with ACE via hydrogen bonds. In conclusion, we identified that CSE exhibited anti-hypertensive effects in SHRs via the inhibition of ACE, the target of most anti-hypertensive drugs. In addition, an ACE inhibitory phytopeptide CSBp5 that decreased SBP levels in rats was newly identified. Our findings supported the ethnomedical use of corn silk tea on hypertension. Moreover, the identification of ACE inhibitory phytopeptide in corn silk further strengthened our findings.


Introduction
Hypertension is the most common serious chronic health problem in the world. The World Health Organization reports that increased blood pressure affects 1.13 billion people worldwide and the prevalence in females aged ≥18 years was around 20% and in males around 24% in 2015. Moreover, hypertension is a major risk factor for the development of stroke, heart failure, coronary artery disease, and renal failure [1]. Renin-angiotensin-aldosterone system (RAAS) is a well known mechanism that controls the blood pressure by regulating the volume of fluid in the body. Angiotensin-converting inhibited the ACE activity and exhibited anti-hypertension effects in SHRs. In addition, we newly identify an ACE inhibitory phytopeptide in corn silk that interacted with the substrate-binding channel of ACE and consequently decreased systolic blood pressure (SBP) levels in rats.

CSE Reduced Blood Pressure In Vivo and Inhibited ACE Activity In Vitro
To analyze whether CSE exhibited anti-hypertensive effects, we treated SHRs with 10 mg/kg captopril or various amounts of CSE orally. Tail SBP was measured at 0 and 1 h. As shown in Figure 1a, the SBP level of captopril group was 47.6 ± 4.34 mmHg lower than that of the blank group. Oral administration of CSE significantly reduced the SBP levels in a dose-dependent manner. The SBP level of 10 mg/kg CSE was 26.57 ± 9.03 mmHg lower than that of the blank group.
Molecules 2019, 24, x FOR PEER REVIEW 3 of 14 applied to evaluate the anti-hypertensive effects of peptides. Furthermore, molecular docking was carried out to clarify the interaction between ACE and anti-hypertensive peptides in corn silk. Our data showed that the boiling water extract of corn silk inhibited the ACE activity and exhibited anti-hypertension effects in SHRs. In addition, we newly identify an ACE inhibitory phytopeptide in corn silk that interacted with the substrate-binding channel of ACE and consequently decreased systolic blood pressure (SBP) levels in rats.

CSE Reduced Blood Pressure In Vivo and Inhibited ACE Activity In Vitro
To analyze whether CSE exhibited anti-hypertensive effects, we treated SHRs with 10 mg/kg captopril or various amounts of CSE orally. Tail SBP was measured at 0 and 1 h. As shown in Figure  1a, the SBP level of captopril group was 47.6 ± 4.34 mmHg lower than that of the blank group. Oral administration of CSE significantly reduced the SBP levels in a dose-dependent manner. The SBP level of 10 mg/kg CSE was 26.57 ± 9.03 mmHg lower than that of the blank group. Figure 1. Effects of CSE on blood pressure and ACE activity. (a) Anti-hypertensive effects of CSE in rats. SHRs were orally given with 10 mg/mL captopril or various dosages of CSE. Tail SBP was measured at 0 and 1 h. Data are expressed as changes in SBP (mmHg). Values are mean ± standard error (n = 6). ** p < 0.01, *** p < 0.001, compared with blank. (b) ACE activity assay. Various amounts of captopril or CSE were mixed with serum ACE and substrates (H-HL or Z-FHL). The hydrolyzed substrates were then labeled with fluorescence and measured using a fluorometer. Data are expressed as relative ACE activity (%), which is presented as the comparison with the fluorescence relative to blank. Values are mean ± standard error (n = 6). SHRs were orally given with 10 mg/mL captopril or various dosages of CSE. Tail SBP was measured at 0 and 1 h. Data are expressed as changes in SBP (mmHg). Values are mean ± standard error (n = 6). ** p < 0.01, *** p < 0.001, compared with blank. (b) ACE activity assay. Various amounts of captopril or CSE were mixed with serum ACE and substrates (H-HL or Z-FHL). The hydrolyzed substrates were then labeled with fluorescence and measured using a fluorometer. Data are expressed as relative ACE activity (%), which is presented as the comparison with the fluorescence relative to blank. Values are mean ± standard error (n = 6).
We further analyzed the effects of CSE on the ACE activity using H-HL and Z-FHL substrates. As shown in Figure 1b, captopril inhibited the hydrolysis of H-HL and Z-FHL substrates by ACE in a dose-dependent manner. CSE also suppressed the ACE activity in a dose-dependent manner. The hydrolysis of H-HL and Z-FHL substrates by ACE was inhibited by CSE, with IC 50 values of 53.95±0.58 µg/mL and 87.26±2.68 µg/mL, respectively. These findings suggested that CSE inhibited the ACE activity and sequentially reduced SBP levels in rats.

Identification of Bioactive Peptides with Blood Pressure Lowering-Potentials in CSE
The well-known ACE inhibitors, such as captopril, lisinopril and enalapril, are amino-acid or dipeptide drugs. Moreover, CSE was a protein-rich extract and the protein content of CSE was 10.86%. Therefore, we analyzed protein profiles of CSE by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis . As shown in Figure 2a, CSE was composed of various proteins, with the molecular weight ranging from 10 to 100 kDa. 2-DE analysis showed that there were several visible protein spots and some smears in the gels. Eleven protein spots with neutral pH were further excised from stained gels, digested by gastrointestinal protease (trypsin), and identified using LC-MS/MS analysis. The identified protein spots of CSE are summarized in Table 1. We further analyzed the effects of CSE on the ACE activity using H-HL and Z-FHL substrates. As shown in Figure 1b, captopril inhibited the hydrolysis of H-HL and Z-FHL substrates by ACE in a dose-dependent manner. CSE also suppressed the ACE activity in a dose-dependent manner. The hydrolysis of H-HL and Z-FHL substrates by ACE was inhibited by CSE, with IC50 values of 53.95±0.58 μg/mL and 87.26±2.68 μg/mL, respectively. These findings suggested that CSE inhibited the ACE activity and sequentially reduced SBP levels in rats.

Identification of Bioactive Peptides with Blood Pressure Lowering-Potentials in CSE
The well-known ACE inhibitors, such as captopril, lisinopril and enalapril, are amino-acid or dipeptide drugs. Moreover, CSE was a protein-rich extract and the protein content of CSE was 10.86%. Therefore, we analyzed protein profiles of CSE by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and two-dimensional electrophoresis . As shown in Figure  2a, CSE was composed of various proteins, with the molecular weight ranging from 10 to 100 kDa. 2-DE analysis showed that there were several visible protein spots and some smears in the gels. Eleven protein spots with neutral pH were further excised from stained gels, digested by gastrointestinal protease (trypsin), and identified using LC-MS/MS analysis. The identified protein spots of CSE are summarized in Table 1.   A total of 133 peptide spectra matched to aforementioned proteins was screened using PeptideRanker. Top 10% of peptides, which had cut-off scores of approximately 0.8, were considered as potential corn silk bioactive peptides (CSBps) (Figure 2b). The MS data of these peptides were shown in Table 2. These peptides were further subjected to BIOPEP database to calculate the potential ACE inhibitor activity. Top 5 peptides with potential ACE inhibitor activity were CSBp9 (GLIYPPFSNIR), CSBp10 (EPFIRPPR), CSBp11 (MNVPPGPFMAR), CSBp5 (SKFDNLYGCR), and CSBp7 (AMPTFFLIK). Docking analysis was further performed to evaluate the interaction between ACE and CSBps. As shown in Table 2, CSBp5 had a highest docking score, followed by CSBp1, CSBp7, and CSBp11. CSBp9 displayed a largest interface area of peptide-ACE complex, followed by CSBp5, CSBp1, and CSBp10. Because CSBp5 displayed a potential ACE inhibitor activity and has a highest docking score with ACE interaction, CSBp5 was synthesized for in vitro ACE activity assay and in vivo hypertensive assay.

CSBp5 Inhibited ACE Activity In Vitro and Reduced Blood Pressure In Vivo
Various amounts of CSBp5 were mixed with mouse sera in the presence of H-HL or Z-FHL substrate, and the ACE activity was determined by measuring the fluorescence-labeled hydrolyzed substrates.
As shown in Figure 3a, captopril inhibited the hydrolysis of both H-HL and Z-FHL substrates by ACE in a dose-dependent manner. The IC 50 values of captopril were 0.68 ± 0.09 µM (H-HL substrate) and 1.08 ± 0.09 µM (Z-FHL substrate). CSBp5 also inhibited the ACE activity and the inhibition displayed a dose-dependent manner. The IC 50 value of CSBp5 for the hydrolysis of H-HL by ACE was 44.11 ± 1.04 µM, while IC 50 value for the hydrolysis of Z-FHL was 81.71 ± 1.06 µM. Moreover, our findings suggested that CSBp5 exhibited a more effective inhibition on the C-domain of ACE. Anti-hypertensive effects of CSBp5 were further evaluated by administering (intraperitoneally) various amounts of CSBp5 in SHR. Tail SBP was measured 1 h after injection. As shown in Figure 3b, a slight decrease of SBP was observed 1 h later in blank group, while a significant decrease (52.83±19.06 mmHg) was observed in captopril group. Intraperitoneal injection of CSBp5 decreased SBP levels in a dose-dependent manner. A significant decrease (28.33 ± 12.5 mmHg injection) was observed in 10 µmol/kg CSBp5 group. Oral administration of CSBp5 also decreased the SBP levels in a dose-dependent manner and a significant decrease (36.78 ± 13.25 mmHg) was observed in 10 µmol/kg CSBp5 group (Figure 3c). Time-course effect of anti-hypertensive ability of CSBp5 was further evaluated in SHR. SHRs were orally given with 10 µmol/kg captopril or CSBp5, and the tail SBP was measured at 0, 1, 2, 4, and 6 h. As shown in Figure 3c, oral administration of water (blank) did not affect SBP levels. However, administration of captopril and CSBp5 caused a significant decrease in SBP, with SBP falling by approximately 60 mmHg and 30 mmHg, respectively, at 2 h. Moreover, the decrease SBP level by CSBp5 was persistent during a 6-h measurement. The changes in diastolic blood pressures were also consistent with the changes in SBP (Supplementary Materials Figure S1). Therefore, these data suggested that CSBp5 inhibited ACE activity and decreased SBP levels in rats.

Interaction between CSBp5 and ACE by Docking Analysis
The possible ACE inhibitory mechanism of CSBp5 was investigated by molecular docking in silico. ACE comprises two similar protein domains (N-and C-domains) and C-domain of ACE is mainly responsible for angiotensin II formation [22]. In addition, our data showed that the

Interaction between CSBp5 and ACE by Docking Analysis
The possible ACE inhibitory mechanism of CSBp5 was investigated by molecular docking in silico. ACE comprises two similar protein domains (N-and C-domains) and C-domain of ACE is mainly responsible for angiotensin II formation [22]. In addition, our data showed that the selectivity of CSBp5 for C-domain versus N-domain was approximately 2-fold. The crystal structure of C-domain of human ACE (PDB ID: 4APH) was therefore applied as a target for docking analysis.
As shown in Figure 4, CSBp5 occupied the substrate-binding channel of ACE. Moreover, CSBp5 formed potential interactions with residues Asn277, Gln281, Thr282, Thr302, His353, Asn374, His513, Ser516, Ser517, and Tyr523 of ACE through hydrogen bonds. Four residues, including Gln281, His353, His513 and Tyr523, were commonly observed among CSBp5-ACE and angiotensin II-ACE interaction [23]. The distance between Gln281 of ACE and Asp4 of CSBp5 was 2.99 Å, while the distances between His353, His513, and Tyr523 of ACE and Gly8 CSBp5 were 2.85, 3.29, and 3.06 Å, respectively. These findings suggested that CSBp5 might interact with the substrate-binding channel of ACE and prevent angiotensin I from entering the catalytic pocket of ACE. As shown in Figure 4, CSBp5 occupied the substrate-binding channel of ACE. Moreover, CSBp5 formed potential interactions with residues Asn277, Gln281, Thr282, Thr302, His353, Asn374, His513, Ser516, Ser517, and Tyr523 of ACE through hydrogen bonds. Four residues, including Gln281, His353, His513 and Tyr523, were commonly observed among CSBp5-ACE and angiotensin II-ACE interaction [23]. The distance between Gln281 of ACE and Asp4 of CSBp5 was 2.99 Å, while the distances between His353, His513, and Tyr523 of ACE and Gly8 CSBp5 were 2.85, 3.29, and 3.06 Å, respectively. These findings suggested that CSBp5 might interact with the substrate-binding channel of ACE and prevent angiotensin I from entering the catalytic pocket of ACE.

Discussion
Although corn silk has been used as an aqueous decoction for anti-hypertensive healthcare in folk medicine, there are few reported studies on the certification of anti-hypertensive effects and mechanisms of corn silk in human subjects or animal models. Martín et al. [24] reported that intravenous injection of 1.342 mg/kg boiling dialysate of corn silk decreases diastolic blood pressure by 63.8% ± 33.6% in normotensive anaesthetized dogs. George and Idu [20] reported that oral administration of 260 mg/kg corn silk aqueous extract reduces the intraocular pressure in eyes with ocular hypertension and lowers the blood pressure in systemic and non-systemic hypertensive subjects. Because the high potassium content is observed in the corn silk extract, and diuretic and uricosuric properties have been discovered in corn silk, they proposed that the fall in blood pressure by corn silk may result from the diuretic activity or direct vasodilatation [25]. Anti-hypertensive activities of CSE were analyzed in SHRs by tail-cuff method in this study. Telemetric blood pressure monitoring system is a direct and effective method for measuring blood pressure of laboratory animals. However, implantation of a radio transmitter is required for this method. Moreover, the

Discussion
Although corn silk has been used as an aqueous decoction for anti-hypertensive healthcare in folk medicine, there are few reported studies on the certification of anti-hypertensive effects and mechanisms of corn silk in human subjects or animal models. Martín et al. [24] reported that intravenous injection of 1.342 mg/kg boiling dialysate of corn silk decreases diastolic blood pressure by 63.8% ± 33.6% in normotensive anaesthetized dogs. George and Idu [20] reported that oral administration of 260 mg/kg corn silk aqueous extract reduces the intraocular pressure in eyes with ocular hypertension and lowers the blood pressure in systemic and non-systemic hypertensive subjects. Because the high potassium content is observed in the corn silk extract, and diuretic and uricosuric properties have been discovered in corn silk, they proposed that the fall in blood pressure by corn silk may result from the diuretic activity or direct vasodilatation [25]. Anti-hypertensive activities of CSE were analyzed in SHRs by tail-cuff method in this study. Telemetric blood pressure monitoring system is a direct and effective method for measuring blood pressure of laboratory animals. However, implantation of a radio transmitter is required for this method. Moreover, the cost of a telemetry apparatus is quite high. Tail-cuff sphygmomanometer has been used as an indirect method to monitor blood pressure in rats for many years. Although multiple factors, such as stress and temperature, may affect the measurement, previous report indicated that tail-cuff method is as good as telemetry for measuring blood pressure in the conscious rats [26]. We found that corn silk extract displayed anti-hypertensive effects in SHRs. Moreover, in vitro serum ACE activity was inhibited by corn silk in a dose-dependent manner. These findings suggested that corn silk exhibited anti-hypertensive abilities via the inhibition of ACE, the target of anti-hypertensive pharmaceutical drugs.
CSE was a protein-rich extract and the protein content of CSE was 10.86%. Moreover, the well-known ACE inhibitors are amino acid or dipeptide drugs. Therefore, we proposed that there were bioactive peptides responsible for the anti-hypertensive activities of CSE. Proteomics and bioinformatics analyses were further applied to identify the anti-hypertensive bioactive peptides in CSE. Peptides with anti-hypertensive potentials have been determined from corn. For example, dipeptide Ala-Tyr (AY) has been identified from corn gluten meal, a major byproduct of corn wet milling. AY affects the ACE activity with an IC 50 of 14.2 µM and a maximal reduction (9.5 mmHg) of SBP is observed 2 h after oral administration of 50 mg/kg AY in SHRs [27]. Several corn peptides have been identified from zein, an alcohol soluble protein present in corn gluten meal. Tripeptide (Leu-Arg-Pro, Leu-Ser-Pro, and Leu-Gln-Pro), identified from thermolysin-hydrolyzed zein, inhibited the ACE activity in vitro with IC 50 values of 0.25-1.9 µM. Moreover, thermolysin hydrolysate (5 g/kg) of zein reduces SBP (14 mmHg) at 6 h after oral administration in SHRs [28]. Oral administration of corn peptides (10 mg/kg) from hydrolyzed zein decreased SBP (42.5 mmHg) in SHRs at 8 h. Further HPLC-MS/MS analysis identified that a tetrapeptide Met-Ile/Leu-Pro-Pro exhibited effective ACE inhibitory activities with an IC 50 of 70.32 µg/mL (155 µM) in vitro [29]. Interestingly, we did not find the presence of these corn peptides in the aqueous extract of corn silk by LC-MS/MS analysis. This might be because the previously reported peptides were from protein digests or the previously reported extract was not like the sample used in this work. Moreover, our data showed that CSBp5 inhibited the ACE activity with an IC 50 value of 44.11 ± 1.04 µM and decreased SBP levels (28.33 ± 12.5 mmHg injection; 36.78 ± 13.25 mmHg oral) at 1 h after administration of 10 µmol/kg (12 mg/kg) CSBp5. Therefore, our study first identified the presence of anti-hypertensive peptide (CSBp5) in corn silk. Furthermore, in comparison of ACE inhibitory peptides in corn, our findings suggested that CSBp5 showed more effective ACE inhibitory activity and anti-hypertensive abilities in SHRs. Moreover, our study showed that CSBp5, like captopril, was an ACE inhibitory peptide. ACE inhibition has been demonstrated in both healthy human subjects and animals by showing that the elevation of blood pressure caused by exogenously administered angiotensin I was attenuated or abolished by captopril. Therefore, we expect that CSBp5 may reduce angiotensin I-induced SBP in normal animal.
CSBp5 was an ACE inhibitory peptide that exhibited a more effective inhibition on the C-domain of ACE. Somatic ACE is composed of two similar catalytic domains (N-and C-domains). Both domains are efficient for the cleavage of angiotensin I. However, RXP407, an ACE N-domain selective inhibitor, displays no effect on blood pressure, suggesting that C-domain is the dominant site for angiotensin II formation [22,23]. Moreover, previous study indicated that C-domain selective ACE inhibitors are likely to reduce blood pressure by lowering angiotensin II and have improved side effect profiles [30]. Docking analysis was therefore performed to elucidate the interaction between CSBp5 and C-domain of ACE. The crystal structure of human C-domain somatic ACE in complex with angiotensin II has been elucidated [23]. The co-crystal structure showed that angiotensin II (Asp-Arg-Val-Tyr-Ile-His-Pro-Phe) can be observed in the substrate-binding channel of ACE. The main interacting residues at the ACE active site are divided into three substrate binding pockets S1 (Ala354, Glu384 and Tyr523), S2' (Gln281, Tyr520, Lys511, His513 and His353), and S1' (Glu162) [31]. Ile5 and His6 residues of angiotensin II interact with Ala354 residue of S1 pocket and all the active site residues of S2' pocket [23]. In this study, we found that CSBp5 (Ser-Lys-Phe-Asp-Asn-Leu-Tyr-Gly-Cys-Arg), like angiotensin II, was located in the substrate-binding channel of ACE. CSBp5 exhibited hydrogen bonds with 10 amino acid residues of ACE. Asp4 and Tyr7 residues of CSBp5 interacted with Tyr523 residue of S1 pocket, and Gln281, His513, and His353 active site residues of S2' pocket. Therefore, we propose that CSBp5 might be a competitive inhibitor by preventing substrates from binding to the active sites of ACE. Nevertheless, proteins are usually digested to amino acid residues or small peptides by pepsin in the stomach and pancreatin in the small intestine after ingestion. Endocytosis, phagocytosis, transcytosis, direct penetration, and paracellular transport are usually responsible for the transport of peptides in the intestinal epithelial [32]. The detailed gastrointestinal transport mechanism of CSBp5 after ingestion remained to be further elucidated. In addition, short half-life is one of the key challenges of therapeutic peptides. Peptide database covering 1,193 unique peptides shows that the half-life of these peptides ranges from 60 sec to 86,400 sec [33]. The exact half-life of CSBp5 needs to be verified by further experiments.

Chemicals
All chemicals, except indicated, were purchased from Sigma-Aldrich (

Animal Experiments
Male SHRs (200-250 g, 10 week-old) were purchased from National Laboratory Animal Center (Taipei, Taiwan). Rats were maintained under a 12 h day/12 h night cycle and had free access to water and food. Rat experiments were conducted under ethics approval from China Medical University Animal Care and Use Committee (Permit No. 104-75-N).
SHRs with tail SBP ≥ 180 mmHg were randomly divided into five groups with six rats per group. Rats were given intraperitoneally or orally with 400 µL of distilled water (blank), captopril (Catalog No. C4042), or various dosages of CSE or peptide (dissolved in water). SBP was measured by a tail-cuff apparatus (Non-Invasive Blood Pressure System, Panlab, Barcelona, Spain). Rats were placed in a warm holder kept at 37 ± 1 • C for 10 min before the measurement. Changes in SBP were calculated by subtracting SBP at indicated time point from SBP at 0 h.

ACE Activity Assay
ACE activity assay was performed as described previously with a slight modification [34]. Mouse sera collected from normal BALB/c mice were used as the source of soluble somatic ACE. ACE activity in mouse serum was determined as described previously [35]. One unit of ACE converts 1 µmol HL/min at 37 • C and the ACE unit in mouse serum was 2 U/µL. A 25-µL reaction mixture, containing 2 µL mouse serum, indicated concentrations of inhibitors (captopril, CSE, or peptide), 400 mM boric acid (pH 8.3) and 300 mM sodium chloride, were incubated at 37 • C for 20 min. Ten microliters of 7 mM H-HL or 3.5 mM Z-FHL were added and the mixture was incubated at 37 • C for 2 h. The ACE activity was then stopped by the addition of 150 µl of 0.34 M NaOH. The free N-terminus of product HL was fluorogenically labeled with o-phthaldialdehyde (OPA, Catalog No. P1378) by adding 20 µl of 20 mg/mL OPA and incubating at room temperature for 10 min. The reaction was then stopped by the addition of 50 µL of 3 M HCl and the fluorescent product was measured using a fluorometer (Fluoroskan Ascent FL, Thermo Fisher, Waltham, MA, USA) with an excitation wavelength at 355 nm and an emission wavelength at 535 nm. To correct the intrinsic fluorescence of mouse serum, blank was performed according to the aforementioned procedures without inhibitors. Relative ACE activity (%) was calculated as: (fluorescence intensity of inhibitor/fluorescence intensity of blank) × 100. (1) IC 50 represents the concentration of the drug that is required for 50% inhibition in vitro.

2-DE and LC-MS/MS Analysis
Protein components of CSE were identified by 2-DE and LC-MS/MS as described previously [36]. Briefly, CSE (200 µg protein) was applied to IPG strips (7 cm, pH 3-10) and isoelectric focusing was performed using a Protean IEF Cell (Bio-Rad, Hercules, CA, USA). Focused IPG strips were separated by SDS-PAGE and protein spots on the gels were visualized by Coomassie Brilliant Blue R-250. Protein spots were then excised from stained gels, digested by trypsin, and identified using an Ultimate capillary LC system (LC Packings, Amsterdam, The Netherlands) coupled to a QSTAR ® XL quadrupole-time-of-flight mass spectrometer (Applied Biosystem/MDS Sciex, Foster City, CA, USA), which was served by Biotechnology Center, National Chung Hsing University (Taichung, Taiwan) [37]. MS/MS data were matched against green plant (Viridiplantae) taxonomy using the MASCOT search program [38]. Protein score (Table 1) reflects the combined scores of all observed mass spectra that can be matched to amino acid sequences within that protein. In general, a higher score indicates a more confident match. Peptide score (Table 2) is a measure of how well the observed MS/MS spectrum matches to the stated peptide. In this study, ion scores >38 indicate homology and ion scores >50 indicate identity (p < 0.05) [38].

Bioinformatics Analysis
Peptides identified by LC-MS/MS were screened by PeptideRanker, a bioinformatics tool for the prediction of bioactive peptides based on a novel N-to-1 neural network [39]. Peptides with the score ≥ 0.8 were selected as potential bioactive peptides and further subjected to BIOPEP database to calculate the potential ACE inhibitor activity of peptide [40].

Preparation of Synthetic Peptides
Peptides were synthesized in solid phase using Fmoc and Noc chemistry (LifeTein, Somerset, NJ, USA). The purities and the amino acid sequences of peptides were identified by high-performance liquid chromatography and MS, respectively. The peptides with purities ≥ 95% were used in this study.

Docking Analysis
Docking analysis was performed by PatchDock [41]. The crystal structure of C-domain of human ACE (PDB ID: 4APH) was obtained from protein data bank. Chain A of 4APH structure was chosen as a target using the target selection tab in PatchDock. The structures of peptides were generated using PEPstrMOD [42] and saved as a pdf format. The pdb files were uploaded using the ligand molecular selection tab in PatchDock. Enzyme-inhibitor complex type was selected to restrict the search space in the cavities of ACE structure. The binding mode and binding affinity were evaluated by the geometric shape complementarity score. All docking structures were generated by UCSF Chimera [43]. The interaction of residues between peptide and ACE was analyzed by LigPlot [44].

Statistical analysis
Data were presented as mean ± standard error. Data were analyzed by one-way ANOVA and post hoc Bonferroni test using SPSS Statistics v20 (IBM, Armonk, NY, USA). A p-value < 0.05 was considered as statistically significant

Conclusions
Corn silk has no significant adverse effects in rats orally given corn silk extract for 90 consecutive days [45]. Subchronic oral administration of corn silk extract shows the no-observed-adverse-effect level is 10 g/kg/day for both male and female mice. Moreover, micronucleus assay and sperm malformation assay showed corn silk is not a genotoxic substance [46]. These studies suggested that corn silk tea is an herbal remedy with low toxicity. In this study, we identified that the boiling water extract of corn silk exhibited anti-hypertension effects in SHRs via the inhibition of ACE, the target of anti-hypertensive drugs. In addition, an ACE inhibitory phytopeptide CSBp5 that decreased SBP levels in rats was identified in corn silk. Therefore, our findings provided a reasonable explanation on why corn silk tea displays anti-hypertensive effects in folk medicine. Moreover, the identification of ACE inhibitory phytopeptide in corn silk extract further strengthened our findings.