2-Alkyl-4-hydroxyquinolines from a Marine-Derived Streptomyces sp. Inhibit Hyphal Growth Induction in Candida albicans

Four 2-alkyl-4-hydroxyquinoline derivatives (1–4) were isolated from a semisolid rice culture of the marine-derived actinomycete Streptomyces sp. MBTG13. The structures of these compounds were elucidated by a combination of spectroscopic methods, and their data were in good agreement with previous reports. Compounds 1 and 2 exhibited weak to moderate antibacterial activity against pathogenic bacteria. Unexpectedly, we found that compound 1 acted as a potent inhibitor of hyphal growth induction in the dimorphic fungus Candida albicans, with an IC50 value of 11.4 μg/mL. Growth experiments showed that this compound did not inhibit yeast cell growth, but inhibited hyphal growth induction. Semi-quantitative reverse transcription (RT)-PCR analysis of hyphal-inducing signaling pathway components indicated that compound 1 inhibited the expression of mRNAs related to the cAMP-Efg1 pathway. The expression of HWP1 and ALS3 mRNAs (hypha-specific genes positively regulated by Efg1, an important regulator of cell wall dynamics) was significantly inhibited by the addition of compound 1. These results indicate that compound 1 acts on the Efg1-mediated cAMP pathway and regulates hyphal growth in Candida albicans.


Introduction
Candida albicans is an important human fungal pathogen that can reversibly transition between two distinct morphological forms: yeast and filamentous hypha [1,2]. Furthermore, the morphological transition ability of the organism contributes to its virulence [3], and hyphal development is closely associated with the dissemination of, and tissue invasion by, C. albicans. Hyphal development in C. albicans is triggered by various in vitro environmental signals such as neutral pH, nutrient-poor media, high temperatures, a high ratio of CO 2 , and serum exposure [4]. In addition to environmental signals, the morphological transition of C. albicans is controlled by a complex network of signaling pathways, including the Cph1-mediated MAPK pathway and the Efg1-mediated cAMP pathway. Ras1 likely acts upstream of both pathways as an important regulator of hyphal development [2].
In our continuing search for bioactive secondary metabolites from marine-derived actinomycetes, we characterized a strain, MBTG13, collected from marine sediment from Jeju Island, Republic of Korea, identified as Streptomyces sp. by its 16S rDNA. An organic extract of a semisolid rice culture of this strain exhibited weak antibacterial activity (minimum inhibitory concentration 64 µg/mL) against two pathogenic bacteria (Staphylococcus aureus and Salmonella enterica). Activity-guided separation of the extract employing diverse chromatographic methods led to the isolation of 2-alkyl-4-hydroxyquinoline derivatives 1-4. In this study, we discovered that these compounds, especially compound 1, were inhibitors of filamentous growth induction in the dimorphic fungus C. albicans. This is the first study to identify the mechanistic effect of this compound on C. albicans morphogenesis.

Taxonomy and Phylogenetic Analysis of MBTG13
The 16S rDNA of strain MBTG13 was amplified by polymerase chain reaction (PCR) and sequenced. After a basic logic alignment search tool (BLAST) sequence comparison, strain MBTG13 showed 99% identity to Streptomyces luridiscabiei (GenBank accession number: NR_025155). Thus, this strain was designated as Streptomyces sp. MBTG13 (GenBank accession number: MK408429). The phylogenetic tree that was generated by the neighbour-joining and maximum likelihood methods based on the 16S rDNA sequence revealed the evolutionary relationships of strain MBTG13 with a group of known Streptomyces species (Figure 1).
In our continuing search for bioactive secondary metabolites from marine-derived actinomycetes, we characterized a strain, MBTG13, collected from marine sediment from Jeju Island, Republic of Korea, identified as Streptomyces sp. by its 16S rDNA. An organic extract of a semisolid rice culture of this strain exhibited weak antibacterial activity (minimum inhibitory concentration 64 μg/mL) against two pathogenic bacteria (Staphylococcus aureus and Salmonella enterica). Activity-guided separation of the extract employing diverse chromatographic methods led to the isolation of 2-alkyl-4-hydroxyquinoline derivatives 1-4. In this study, we discovered that these compounds, especially compound 1, were inhibitors of filamentous growth induction in the dimorphic fungus C. albicans. This is the first study to identify the mechanistic effect of this compound on C. albicans morphogenesis.

Taxonomy and Phylogenetic Analysis of MBTG13
The 16S rDNA of strain MBTG13 was amplified by polymerase chain reaction (PCR) and sequenced. After a basic logic alignment search tool (BLAST) sequence comparison, strain MBTG13 showed 99% identity to Streptomyces luridiscabiei (GenBank accession number: NR_025155). Thus, this strain was designated as Streptomyces sp. MBTG13 (GenBank accession number: MK408429). The phylogenetic tree that was generated by the neighbour-joining and maximum likelihood methods based on the 16S rDNA sequence revealed the evolutionary relationships of strain MBTG13 with a group of known Streptomyces species (Figure 1). Bootstrap was performed with 1000 replicates. The Kimura two-parameter model was used for measuring distance. Bar indicates 0.5% sequence divergence.

Effects of Compounds 1-4 on C. albicans Morphogenesis
The effects of isolated compounds 1-4 on C. albicans SC5314 growth and morphogenesis were evaluated. First, to evaluate the effects of these compounds on C. albicans yeast growth, the cells were

Effects of Compounds 1-4 on C. albicans Morphogenesis
The effects of isolated compounds 1-4 on C. albicans SC5314 growth and morphogenesis were evaluated. First, to evaluate the effects of these compounds on C. albicans yeast growth, the cells were grown in glucose salt (GS) medium supplemented with 100 µg/mL of test compound at 28 • C, and the optical density at 660 nm (OD 660 ) of each sample was measured at each specific time interval. Compounds 1-4 at 100 µg/mL did not inhibit yeast cell growth in C. albicans (Figure 3a). To evaluate the effects of compounds 1-4 on the hyphal growth of C. albicans, approximately 5 × 10 6 cells/mL were added to GS medium supplemented with the test compounds at different concentrations and incubated at 37 • C. At each time point, the morphology of approximately 200 cells was determined by light microscopy (Figure 3b). Under these conditions, >90% of C. albicans cells converted to the hyphal form after 4 h of incubation. Cultures treated with compounds 1-4 exhibited concentration-dependent inhibition of the hyphal form of C. albicans. Among these compounds, compound 1 exhibited potent inhibitory activity, with an IC 50 of 11.4 µg/mL. Oxidation of 4-hydroxyquinoline moiety (2) (Figure 2) led to a loss of inhibitory activity compared to compound 1. With regard to the effect of alkyl chain length, for a given compound 1, extension of the chain by one methyl unit (3) resulted in a dramatic decrease in inhibitory effect upon hyphal formation at the highest concentration tested (50% at 100 µg/mL). From these results, it is apparent that the inhibitory potency and selectivity of 2-alkyl-4-hydroxyquinoline derivatives (1)(2)(3)(4) are sensitively dependent upon the chain length, as well as to substitutions on the 4-hydroxyquinoline template. Taken together, these data suggest that 2-alkyl-4-hydroxyquinoline derivatives regulate the hyphal formation process of C. albicans without interfering with its yeast form proliferation.
Mar. Drugs 2019, 17, x FOR PEER REVIEW 4 of 11 grown in glucose salt (GS) medium supplemented with 100 μg/mL of test compound at 28 °C, and the optical density at 660 nm (OD660) of each sample was measured at each specific time interval. Compounds 1-4 at 100 μg/mL did not inhibit yeast cell growth in C. albicans ( Figure 3A). To evaluate the effects of compounds 1-4 on the hyphal growth of C. albicans, approximately 5 × 10 6 cells/mL were added to GS medium supplemented with the test compounds at different concentrations and incubated at 37 °C. At each time point, the morphology of approximately 200 cells was determined by light microscopy ( Figure 3B). Under these conditions, >90% of C. albicans cells converted to the hyphal form after 4 h of incubation. Cultures treated with compounds 1-4 exhibited concentration-dependent inhibition of the hyphal form of C. albicans. Among these compounds, compound 1 exhibited potent inhibitory activity, with an IC50 of 11.4 μg/mL. Oxidation of 4-hydroxyquinoline moiety (2) (Figure 2) led to a loss of inhibitory activity compared to compound 1. With regard to the effect of alkyl chain length, for a given compound 1, extension of the chain by one methyl unit (3) resulted in a dramatic decrease in inhibitory effect upon hyphal formation at the highest concentration tested (50% at 100 μg/mL). From these results, it is apparent that the inhibitory potency and selectivity of 2-alkyl-4-hydroxyquinoline derivatives (1-4) are sensitively dependent upon the chain length, as well as to substitutions on the 4-hydroxyquinoline template. Taken together, these data suggest that 2-alkyl-4-hydroxyquinoline derivatives regulate the hyphal formation process of C. albicans without interfering with its yeast form proliferation.

Analysis of Gene Expression Related to Hypha-Inducing Signaling Pathways
In C. albicans, hyphal development is mainly regulated by the mitogen-activated protein kinase (MAPK) and cyclic AMP-protein kinase A (cAMP-PKA) pathways, and active Ras1 is required for the regulation of both pathways [2]. The transcription factors Efg1 and Cph1 are activated by distinct upstream signaling pathways. In the case of Efg1, the pathway is based on cAMP, while in the case of Cph1 the pathway depends on a MAPK signaling pathway, with Ras1 stimulating both pathways. To investigate the effect of compound 1 on hypha-inducing signaling pathways in C. albicans, the mRNA expression levels of CPH1, EFG1, GAP1, and HWP1 were determined using gene-specific primers ( Table 2).

Analysis of Gene Expression Related to Hypha-Inducing Signaling Pathways
In C. albicans, hyphal development is mainly regulated by the mitogen-activated protein kinase (MAPK) and cyclic AMP-protein kinase A (cAMP-PKA) pathways, and active Ras1 is required for the regulation of both pathways [2]. The transcription factors Efg1 and Cph1 are activated by distinct upstream signaling pathways. In the case of Efg1, the pathway is based on cAMP, while in the case of Cph1 the pathway depends on a MAPK signaling pathway, with Ras1 stimulating both pathways. To investigate the effect of compound 1 on hypha-inducing signaling pathways in C. albicans, the mRNA expression levels of CPH1, EFG1, GAP1, and HWP1 were determined using gene-specific primers ( Table 2).

CPH1-For
Mar. Drugs 2019, 17, x FOR PEER REVIEW 5 of 11  Semi-quantitative reverse transcription (RT)-PCR showed that the mRNA expression of CPH1 and EFG1 was not repressed in compound 1-treated cells ( Figure 4A). The transcript level of GAP1, which encodes a general amino acid permease and is positively regulated by the transcription factor Cph1 [4] was also unaffected. Importantly, a complete loss of HWP1 mRNA expression occurred with 100 μg/mL of compound 1. HWP1, which encodes a glycosylphosphatidylinositol-anchored cell wall protein [19], is a downstream component of the cAMP-dependent PKA pathway and is Semi-quantitative reverse transcription (RT)-PCR showed that the mRNA expression of CPH1 and EFG1 was not repressed in compound 1-treated cells (Figure 4a). The transcript level of GAP1, which encodes a general amino acid permease and is positively regulated by the transcription factor Cph1 [4] was also unaffected. Importantly, a complete loss of HWP1 mRNA expression occurred with 100 µg/mL of compound 1. HWP1, which encodes a glycosylphosphatidylinositol-anchored cell wall protein [19], is a downstream component of the cAMP-dependent PKA pathway and is positively regulated by the transcription factor Efg1, which is an important regulator of cell wall dynamics [20]. We next investigated the relationship between HWP1 transcript levels and hypha formation in C. albicans cultures grown in GS medium treated with increasing concentrations of compound 1 at 37 • C for 2 h. Figure 4b shows that the level of HWP1 transcript was undetectable in yeast cells grown in GS medium at 28 • C for 2 h. In contrast, under hyphal growth-inducing conditions, the HWP1 transcript level was increased with a decrease in concentration of compound 1, mainly due to the predominant growth of the hyphal population. These results suggest that compound 1 functions on the cAMP-Efg1 pathway, but not the MAPK-Cph1 pathway.

Primer Name
Gene-specific studies and genome-wide analyses have revealed only a small number of hypha-specific genes in C. albicans [21]. These include ALS3, ECE1, HGC1, HWP1, HYR1, RBT1, and RBT4. The most highly expressed gene encodes HWP1, which is one of a small set of hypha-specific genes in C. albicans that include ALS3 (encoding agglutinin-like protein 3) and ECE1 (encoding cell elongation protein 1). Among them, HWP1 and ALS3 encode adhesins and are activated by the transcription regulator Efg1 during hypha formation [22]. Thus, we investigated whether compound 1 also affected HWP1 and ALS3 mRNA levels (Figure 4c). In the absence of compound 1, HWP1 and ALS3 transcripts were undetectable at time zero, but they were strongly expressed from 20 to 80 min. Importantly, compound 1 (100 µg/mL) dramatically reduced the magnitude of HWP1 and ALS3 mRNA expression in compound 1-treated cells, when compared with untreated cells.

Discussion
A chemical investigation of a semisolid rice culture extract of the marine-derived actinomycete Streptomyces sp. MBLG13 led to the isolation of 2-alkyl-4-hydroxyquinoline derivatives (1)(2)(3)(4). Their structures were elucidated based on spectroscopic data and were in good agreement with previous reports. Compounds 1 and 2 exhibited weak to moderate antibacterial activity against pathogenic bacteria. Compound 1 was earlier isolated from Pseudomonas aeruginosa and had antibacterial activity against Gram-positive bacteria [14]. In addition, compounds 1, 2, and 4 were isolated from Pseudomonas methanica KY4634 as 5-lipoxygenase inhibitors [11]. Recently, it was reported that compounds 1-3 exhibited antimalarial activity against Plasmodium falciparum [17]. In this study, we found that compounds 1 and 3 inhibited an essential C. albicans virulence factor, especially against hyphal growth, without inhibiting yeast cell growth. Our initial assumption was that these compounds were inhibitors of hyphal growth induction in C. albicans due to their structural analogies to known quorum sensing signal molecules, including farnesoic acid and farnesol [23,24]. These molecules are secreted into the medium as the cells proliferate and are involved in morphogenesis. Thus, we examined whether these compounds acted as inhibitors of the morphological transition in C. albicans under hypha-inducing conditions. Our results reveal that 2-alkyl-4-hydroxyquinoline derivatives regulate the hyphal formation process of C. albicans without interfering with its yeast form proliferation. The inhibitory potency and selectivity of these compounds are sensitively dependent upon the chain length and to substitutions on the 4-hydroxyquinoline template.
In C. albicans, the positive regulation of hypha-specific gene expression is mainly mediated through the MAPK and cAMP-PKA signaling pathways [1,2]. Transcription factors Efg1 and Cph1 are activated by distinct upstream signaling pathways. In the case of Efg1, the pathway is based on cAMP, while in the case of Cph1 the pathway depends on MAPK signaling. To investigate the networks by which the morphogenesis of C. albicans is controlled by compound 1, we compared the expression levels of these major hypha-inducing signaling pathway components in compound 1-treated cells with untreated control cells. When cells were exposed to compound 1, the mRNA expression of HWP1 and ALS3, hypha-specific genes that are positively regulated by an important regulator of cell wall dynamics (Efg1), were significantly inhibited by the addition of compound 1. In contrast, the transcription of CPH1, EFG1, and GAP1 mRNA was unchanged.
Efg1 and Cph1 are transcription factors whose activity is regulated on a post-transcriptional level. Threonine-206, a phosphorylation site for protein kinase A (PKA) within an Efg1p domain, is essential to promote hyphal induction by environmental factors [25]. Previous studies have examined the role of Efg1 and Cph1 in the repression of hyphal growth by farnesol, a related compound. It has been found that even though these proteins are clearly involved in mediating the farnesol effect, there is no significant change in EFG1 or CPH1 mRNA [26,27]. In our results, EFG1 mRNA levels are regulated during hyphal development, but they were not affected by compound 1, since the magnitude of the changes were similar in the presence and absence of compound 1 (Figure 4a). These results are consistent with those of Kebaara et al. [26]. Together with our results, this suggests that compound 1 does not regulate EFG1 mRNA levels, but at this time, we cannot exclude the possibility that post-translational regulation of Efg1 is affected by compound 1. This study is the first report demonstrating inhibitory activity of C. albicans filamentation by compounds 1-4, and suggests that the 2-alkyl-4-hydroxyquinoline class of compounds may be valuable as antifungal agents to suppress virulence in C. albicans.

General Experimental Procedures
1 H, 13 C, and 2D NMR spectra were measured using a Bruker Avance 600 MHz spectrometer at the National Center for Interuniversity Research Facilities (NCIRF) located in Seoul National University. Mass spectrometric data were obtained at the Korea Basic Science Institute (Daegu, Republic of Korea) and were acquired using a JMS 700 mass spectrometer (JEOL, Tokyo, Japan) using meta-nitrobenzyl alcohol as a matrix for fast atom bombardment mass spectrometry. HPLC was performed using a Spectrasystem p2000 (Thermo Scientific, Waltham, MA, USA) equipped with a Spectrasystem RI-150 refractive index detector. All organic solvents used in the experiment purchased from Fisher Scientific (FairLawn, NJ, USA).

Taxonomic Identification of the Producing Microorganism
The bacterial strain, Streptomyces sp. (strain number MBTG13), was isolated from a marine sediment sample from the shoreline of Jeju Island, Republic of Korea. The strain was identified using standard molecular biological protocols by DNA amplification and sequencing of the internal transcribed spacer region. The genomic DNA extraction was performed using an i-Genomic BYF DNA Extraction Mini Kit (Intron Biotechnology, Seoul, Republic of Korea) according to the manufacturer's protocol. The nucleotide sequence of MBTG13 has been assigned as accession number MK408429 in the GenBank database. The 16S rDNA sequence of this strain showed 99% identity with S. luridiscabiei (GenBank accession number: NR_025155) based on comparison in basic local alignment search tool (BLAST).

Fermentation and Isolation
Strain MBTG13 was cultivated on a yeast extract-peptone-glycerol (YPG) agar plate (5 g of yeast extract, 5 g of peptone, 10 g of glucose, and 16.0 g of agar in 1 L of artificial seawater) for 5 days at 28 • C. It formed brown-yellow colonies with white spore on plates. Agar plugs (1 cm × 1 cm, five pieces each) were inoculated into 100 mL of YPG media for 5 days to obtain seed broth. For the purpose of large-scale fermentation, seed cultures separately were transferred to 24 flasks, each containing 2 g of peptone, 2 g of yeast extract, and 200 g of rice with 200 mL of artificial seawater.
The fermentation in rice media was conducted under static conditions for 6 weeks at 28 • C. A total of 510 g of rice fermentation culture was repeatedly extracted by MeOH (1 L × 3) and CH 2 Cl 2 (1 L × 3). The organic solvents were evaporated to dryness under reduced pressure to gain 8.5 g of total extracts. A total extract (8.5 g) was successively partitioned between n-BuOH (1.6 g) and H 2 O (6.2 g). The n-BuOH fraction was repartitioned using H 2

Antibacterial Activity Assay
The antibacterial activity assay was carried out according to the Clinical and Laboratory Standards Institute (CLSI) method [28]. Gram-positive bacteria (S. aureus ATCC25923, E. faecalis ATCC19433, E. faecium ATCC19434) and Gram-negative bacteria (K. pneumoniae ATCC10031, S. enterica ATCC14028, E. coli ATCC25922) were cultured overnight in Mueller Hinton broth (MHB) at 37 • C. The cells were collected by centrifugation, and washed two times with sterile distilled water. Each test compound was dissolved in dimethyl sulfoxide (DMSO) and diluted with MBH to prepare serial twofold dilutions in the range of 0.06-128 µg/mL. The final DMSO concentration was maintained at 1% by adding DMSO to the medium as guided by the CLSI [28]. In each well of a 96-well plate, 190 µL of MBH containing the test compound was mixed with 10 µL of the broth containing approximately 10 6 colony-forming units (cfu)/mL of test bacterium (final concentration: 5 × 10 4 cfu/mL) adjusted to match the turbidity of a 0.5 MacFarland standard. The plates were incubated for 24 h at 37 • C. The MIC was defined as the lowest concentration of test compound that prevented cell growth. Ampicillin and tetracycline were used as reference compounds.

Antifungal Activity Assay
The antifungal activity assay was performed in accordance with the guidelines in CLSI document M38 [29]. C. albicans SC5314 was cultured on potato dextrose agar (PDA). After incubation for 48 h at 28 • C, yeast cells were harvested by centrifugation and washed twice with sterile distilled water. Aspergillus fumigatus HIC6094, T. rubrum NBRC9185, and T. mentagrophytes IFM40996 were plated on PDA and incubated for 2 weeks at 28 • C. Spores were harvested and washed two times with sterile distilled water. Stock solutions of the compound were prepared in DMSO. Each stock solution was diluted in RPMI 1640 broth (Difco, Livonia, MI, USA) with the concentration range of 0.06-128 µg/mL. The final DMSO concentration was maintained at 1% by adding DMSO to the broth. Harvested cells were adjusted to 0.5 MacFarland standard in RPMI 1640 broth and the diluted cells were added to each well of a 96-well plate with test compound solution. The final inoculum concentration was 10 4 cells/mL in each well. The plates were incubated for 24 h (for C. albicans), 48 h (for A. fumigatus), and 96 h (for T. rubrum and T. mentagrophytes) at 37 • C. The MIC value was determined as the lowest concentration of test compound that fully inhibited cell growth. A culture with DMSO (1%) was used as a solvent control, and a culture supplemented with amphotericin B was used as a positive control.

Candida albicans Strain and Growth Medium
C. albicans strain SC5314 was used for most experiments. To prepare cells, C. albicans cultures were grown at 28 • C with shaking in YPD (1% yeast extract, 2% peptone, and 2% dextrose) medium to the exponential phase, harvested by centrifugation, washed, resuspended in distilled water, and stored for 48 h at 4 • C before use. The hypha-inducing media used in this study was composed of GS medium (5 g of glucose, 0.26 g of Na 2 HPO 4 ·12H 2 O, 0.66 g of KH 2 PO 4 , 0.88 g of MgSO 4 ·7H 2 O, 0.33 g of NH 4 Cl, and 16 µg of biotin per L) [23].

Candida albicans Growth and Morphological Transition Assay
C. albicans SC5314 cells were pregrown in YPD medium at 28 • C up to the exponential phase, washed, and starved in water for 48 h at 4 • C. Stock solutions of compounds 1-4 (4 mg/mL) were prepared in DMSO and added to test media at the prescribed concentrations (final DMSO concentration: 1%). For the yeast cell growth assay, 1 × 10 6 cells/mL were inoculated in GS medium containing test compounds at different concentrations, and the cultures were incubated at 28 • C for 24 h. The number of cells was calculated by measuring the OD 660 of vigorously vortexed cultures at designated time points (every 4 h until 24 h). For the hyphal formation assay, approximately 5 × 10 6 cells/mL were added to GS medium containing test compounds at different concentrations and incubated at 37 • C for 4 h. GS medium with 1% DMSO was used as a negative control. At each time point, the morphology of the cells was determined by light microscopy. A minimum of 200 cells were counted for each sample. Assays were carried out three times, each with three replicates.

Gene Expression Analysis
C. albicans strain SC5314 was cultured in YPD medium at 28 • C on rotary shakers for 24 h, harvested by centrifugation, washed, resuspended in distilled water, and incubated at 4 • C for 48 h before use. GS medium containing compound 1 (25,50, and 100 µg/mL) was added to C. albicans cells, and each sample was incubated at 37 • C for 2 h. Extraction of total RNA was carried out using an RNeasy Mini Kit (Qiagen, San Diego, CA, USA) and 1 µg of extracted total RNA was reverse transcribed into cDNA using the Superscript III First-Strand Synthesis System (Invitrogen, Carlsbad, CA, USA) with oligo (dT) 20 (50 mM) primer. Semi-quantitative RT-PCR was conducted with gene-specific primers for major components of the signaling pathways (Table 2). Signaling pathway components are coded as follows: CPH1 (transcription factor), MAPK pathway; EFG1 (transcription factor), cAMP-protein kinase A complex (PKA) pathway; GAP1, which encodes a general amino acid permease and is positively regulated by Cph1; HWP1 and ALS3, which encode hyphal wall protein 1 and agglutinin-like protein 3, respectively, and are activated by Efg1. The housekeeping gene GPD1 (encoding glyceraldehyde-3-phosphate dehydrogenase) was used as a loading control [30]. PCR conditions were programmed according to the manufacturer's instructions: initial denaturation at 98 • C for 5 min, followed by 30 cycles of denaturation at 98 • C for 10 s, annealing at 55 • C for 30 s, and extension at 72 • C for 1 min, with a final extension at 72 • C for 5 min. ImageJ software (National Institutes of Health, Bethesda, MD, USA) was used for densitometric analyses of the mRNA expression levels.