Synthesis of an Anion Receptor Using 3,6-Diaminophenanthrene as a Scaffold

: The synthesis of phosphate receptors represents an important avenue of research given the ubiquity of phosphate in biological and environmental systems. While many molecular scaffolds suitable for smaller anions are available either commercially or via reported synthetic routes, scaffolds suitable for larger anions such as phosphate are less common. In this work, we present a clear and straightforward synthesis of the basic molecular scaffold 3,6-diaminophenanthrene and of a novel 3,6-bisureidophenanthrene anion receptor prepared from this scaffold. Of the seven synthetic steps using readily available starting materials and reagents, only a single chromatographic purification step was required. The different interactions of the 3,6-bisureidophenanthrene-based anion receptor with phosphate and chloride are demonstrated. We expect that this convenient synthesis of the 3,6-diaminophenanthrene building block will pave the way for applications in many different fields of research, from materials science to supramolecular chemistry.


Introduction
Anions play an instrumental role in a wide range of biological and environmental processes, and, as a result, their recognition by synthetic anion receptors has been an active area of research since the late 1960s [1].The recognition of phosphates is of particular interest due to their high biological relevance, being involved in processes such as energy storage and signal transduction [2,3] and in diseases such as hyperphosphatemia [4].Furthermore, their overabundance in environmental systems has potentially adverse outcomes [5].
Synthetic anion receptors can use different types of interactions, such as hydrogen bonds, halogen bonds, and anion-π interactions.The most effective anion receptors are obtained by the combined action of multiple pre-organized interacting groups [6][7][8].The development of such receptors requires rigid molecular scaffolds, preferably functionalized with amines that can be turned into binding groups such as amides, ureas, thioureas, or squaramides.The distance between these amine groups and their orientation determines the size of the binding site of the receptor.
For smaller anions such as chloride, a wealth of molecular scaffolds has been employed [9][10][11], including 1,8-diaminoanthrancene (1) [12] and ortho-phenylenediamines (2) [13] (Figure 1).For larger anions with a more complex geometry such as phosphate, fewer simple scaffolds are available [14].An example is the diaminocarbazoles reported by Chmielewski et al. (3) [15,16].In our previous work on macrocyclic anion receptors, we have described the synthesis of a 3,6-diaminophenanthrene featuring two hexyloxysubstituents (4) for the synthesis of macrocyclic receptors for phosphate and pyrophosphate [17].Compound 4 was obtained via a Buchwald-Hartwig coupling and its synthesis route featured several difficult and tedious purification steps.Additionally, the two hexyloxy-substituents may not be necessary nor desired in many cases.Examples of reported aromatic diamine scaffolds, for which the anion binding site upon formation of (thio)ureas increases from left to right (1)(2)(3)(4), as well as the aromatic diamine scaffold that is used in this work (11).
In the present work, we present a facile synthetic route to the basic 3,6-diaminophenanthrene building block (11) without additional substituents, using readily available starting materials and reagents.The use of this basic building block for the synthesis of a bis-urea anion receptor is also demonstrated.

Results and Discussion
As part of a larger project aimed at the synthesis of receptors capable of binding phosphate and phosphate derivatives, we set out to develop a molecular scaffold wellsuited for the binding of such phosphates.Molecular mechanics modeling (Figure 2) suggested that a phenanthrene appended with binding moieties at the 3-and 6-positions would provide us with receptors that would have a binding site with a size suitable to accommodate phosphate, as well as a rigid backbone to help ensure a good orientation of the binding moieties and avoid intramolecular H-bonding between the two urea groups.The molecular scaffold required to obtain such a receptor is the corresponding 3,6diaminophenanthrene (11), which was reported in 1968 by Staab et.al. [18], but has seen little utilization since.A synthesis of the precursor 3,6-dinitrophenanthrene (10) from 2iodo-4-nitrobenzaldehyde (8) was reported in 1958 by Bacon and Lindsay [19], as outlined in Scheme 1, and was chosen for our route to diamine 11.To obtain 2-iodo-4-nitrobenzaldehyde (8), we started from commercially available 4-nitroanthranillic acid (5).The first step is a Sandmeyer reaction, starting with a diazotization, followed by a reaction with potassium iodide in a one-pot procedure to yield the corresponding 2-iodo-4-nitrobenzoic acid (6) [20].Next, this carboxylic acid was reduced using diborane generated from NaBH4 and I2 to obtain (2-iodo-4-nitrophenyl)methanol ( 7) [21].The alcohol could then be oxidized using manganese dioxide to obtain 2-iodo-4-nitrobenzaldehyde (8) [21].A classical Ullmann coupling of 2-iodo-4-nitrobenzaldehyde (8) using copper in refluxing DMF then afforded the corresponding biphenyl dicarbaldehyde (9).Finally, a reductive cyclization using hydrazine hydrate in refluxing glacial acetic acid was employed to form the phenanthrene ring system and obtain the desired 3,6-dinitrophenanthrene (10).Notably, only Examples of reported aromatic diamine scaffolds, for which the anion binding site upon formation of (thio)ureas increases from left to right (1-4), as well as the aromatic diamine scaffold that is used in this work (11).
In the present work, we present a facile synthetic route to the basic 3,6-diaminophenanthrene building block (11) without additional substituents, using readily available starting materials and reagents.The use of this basic building block for the synthesis of a bis-urea anion receptor is also demonstrated.

Results and Discussion
As part of a larger project aimed at the synthesis of receptors capable of binding phosphate and phosphate derivatives, we set out to develop a molecular scaffold wellsuited for the binding of such phosphates.Molecular mechanics modeling (Figure 2) suggested that a phenanthrene appended with binding moieties at the 3-and 6-positions would provide us with receptors that would have a binding site with a size suitable to accommodate phosphate, as well as a rigid backbone to help ensure a good orientation of the binding moieties and avoid intramolecular H-bonding between the two urea groups.Examples of reported aromatic diamine scaffolds, for which the anion binding site upon formation of (thio)ureas increases from left to right (1-4), as well as the aromatic diamine scaffold that is used in this work (11).
In the present work, we present a facile synthetic route to the basic 3,6-diaminophenanthrene building block (11) without additional substituents, using readily available starting materials and reagents.The use of this basic building block for the synthesis of a bis-urea anion receptor is also demonstrated.

Results and Discussion
As part of a larger project aimed at the synthesis of receptors capable of binding phosphate and phosphate derivatives, we set out to develop a molecular scaffold wellsuited for the binding of such phosphates.Molecular mechanics modeling (Figure 2) suggested that a phenanthrene appended with binding moieties at the 3-and 6-positions would provide us with receptors that would have a binding site with a size suitable to accommodate phosphate, as well as a rigid backbone to help ensure a good orientation of the binding moieties and avoid intramolecular H-bonding between the two urea groups.The molecular scaffold required to obtain such a receptor is the corresponding 3,6diaminophenanthrene (11), which was reported in 1968 by Staab et.al. [18], but has seen little utilization since.A synthesis of the precursor 3,6-dinitrophenanthrene (10) from 2iodo-4-nitrobenzaldehyde (8) was reported in 1958 by Bacon and Lindsay [19], as outlined in Scheme 1, and was chosen for our route to diamine 11.To obtain 2-iodo-4-nitrobenzaldehyde (8), we started from commercially available 4-nitroanthranillic acid (5).The first step is a Sandmeyer reaction, starting with a diazotization, followed by a reaction with potassium iodide in a one-pot procedure to yield the corresponding 2-iodo-4-nitrobenzoic acid (6) [20].Next, this carboxylic acid was reduced using diborane generated from NaBH4 and I2 to obtain (2-iodo-4-nitrophenyl)methanol (7) [21].The alcohol could then be oxidized using manganese dioxide to obtain 2-iodo-4-nitrobenzaldehyde (8) [21].A classical Ullmann coupling of 2-iodo-4-nitrobenzaldehyde (8) using copper in refluxing DMF then afforded the corresponding biphenyl dicarbaldehyde (9).Finally, a reductive cyclization using hydrazine hydrate in refluxing glacial acetic acid was employed to form the phenanthrene ring system and obtain the desired 3,6-dinitrophenanthrene (10).Notably, only − showing good interaction between both urea groups and the phosphate guest.
the reduction from the carboxylic acid (6) to the alcohol (7) required a chromatographic separation, with the other steps only requiring either filtration or recrystallization.Scheme 1. Synthetic scheme outlining the synthetic route to 3,6-diaminophenanthrene (10) as originally developed by Bacon and Lindsay [19].
Having obtained 3,6-dinitrophenanthrene (10), attention was now turned to the reduction of the nitro groups (Scheme 2).Due to the expected poor stability of the phenanthrene diamine (11), mild methods of reduction such as NH2NH2▪H2O and Pd/C or NaBH4 and NiCl2 were tested.However, while these reagents led to a reduction, very significant amounts of decomposition products were observed, even with reaction times as low as 30 min.Eventually, we found that using iron powder and NH4Cl in a refluxing mixture of ethanol and water afforded the diamine (11) with minimal decomposition and an easy, fast workup in 70% yield.Upon obtaining the pure amine, its very poor stability in the absence of additional stabilizing agents was confirmed, as the initially light brown solid would turn completely black in a few hours, even when stored under a vacuum in the absence of light.Acetonitrile solutions of 11 used for NMR analysis would also change from very slightly colored transparent solutions to completely black and opaque solutions in a few hours.Therefore, the diamine was used directly after its synthesis to form receptor 13 by reacting it with isocyanate 12.The desired compound precipitated from the reaction mixture, requiring only filtration and washing to obtain the pure compound 13.
The receptor was screened for anion binding with tetrabutylammonium (TBA) salts of Cl − and H2PO4 − in DMSO-d6 with 0.5% H2O.Clear downfield changes in the chemical shifts of the signals corresponding to urea H-atoms in the binding site were observed, indicative that both anions interact with 13 (Figure 3).The full titrations are presented in the supporting information (Figures S20 and S21) and show clear indications that the stoichiometry is more complex than a 1:1 receptor-to-anion interaction.For the titrations with Scheme 1. Synthetic scheme outlining the synthetic route to 3,6-diaminophenanthrene (10) as originally developed by Bacon and Lindsay [19].
Having obtained 3,6-dinitrophenanthrene (10), attention was now turned to the reduction of the nitro groups (Scheme 2).Due to the expected poor stability of the phenanthrene diamine (11), mild methods of reduction such as NH 2 NH 2 ■H 2 O and Pd/C or NaBH 4 and NiCl 2 were tested.However, while these reagents led to a reduction, very significant amounts of decomposition products were observed, even with reaction times as low as 30 min.Eventually, we found that using iron powder and NH 4 Cl in a refluxing mixture of ethanol and water afforded the diamine (11) with minimal decomposition and an easy, fast workup in 70% yield.Upon obtaining the pure amine, its very poor stability in the absence of additional stabilizing agents was confirmed, as the initially light brown solid would turn completely black in a few hours, even when stored under a vacuum in the absence of light.Acetonitrile solutions of 11 used for NMR analysis would also change from very slightly colored transparent solutions to completely black and opaque solutions in a few hours.
the reduction from the carboxylic acid (6) to the alcohol (7) required a chromatographic separation, with the other steps only requiring either filtration or recrystallization.Scheme 1. Synthetic scheme outlining the synthetic route to 3,6-diaminophenanthrene (10) as originally developed by Bacon and Lindsay [19].
Having obtained 3,6-dinitrophenanthrene (10), attention was now turned to the reduction of the nitro groups (Scheme 2).Due to the expected poor stability of the phenanthrene diamine (11), mild methods of reduction such as NH2NH2▪H2O and Pd/C or NaBH4 and NiCl2 were tested.However, while these reagents led to a reduction, very significant amounts of decomposition products were observed, even with reaction times as low as 30 min.Eventually, we found that using iron powder and NH4Cl in a refluxing mixture of ethanol and water afforded the diamine (11) with minimal decomposition and an easy, fast workup in 70% yield.Upon obtaining the pure amine, its very poor stability in the absence of additional stabilizing agents was confirmed, as the initially light brown solid would turn completely black in a few hours, even when stored under a vacuum in the absence of light.Acetonitrile solutions of 11 used for NMR analysis would also change from very slightly colored transparent solutions to completely black and opaque solutions in a few hours.Therefore, the diamine was used directly after its synthesis to form receptor 13 by reacting it with isocyanate 12.The desired compound precipitated from the reaction mixture, requiring only filtration and washing to obtain the pure compound 13.
The receptor was screened for anion binding with tetrabutylammonium (TBA) salts of Cl − and H2PO4 − in DMSO-d6 with 0.5% H2O.Clear downfield changes in the chemical shifts of the signals corresponding to urea H-atoms in the binding site were observed, indicative that both anions interact with 13 (Figure 3).The full titrations are presented in the supporting information (Figures S20 and S21) and show clear indications that the stoichiometry is more complex than a 1:1 receptor-to-anion interaction.For the titrations with Therefore, the diamine was used directly after its synthesis to form receptor 13 by reacting it with isocyanate 12.The desired compound precipitated from the reaction mixture, requiring only filtration and washing to obtain the pure compound 13.
The receptor was screened for anion binding with tetrabutylammonium (TBA) salts of Cl − and H 2 PO 4 − in DMSO-d 6 with 0.5% H 2 O. Clear downfield changes in the chemical shifts of the signals corresponding to urea H-atoms in the binding site were observed, indicative that both anions interact with 13 (Figure 3).The full titrations are presented in the supporting information (Figures S20 and S21) and show clear indications that the stoichiometry is more complex than a 1:1 receptor-to-anion interaction.For the titrations with Cl − , the changes in chemical shifts could be fitted to a 1:2 binding model (Figure S20), which gave affinity constants K a(1:1) = 87 M −1 and a K a(1:2) = 17 M −1 .This K a(1:1) value is similar to the one obtained for a simple urea with bis(CF 3 )phenyl groups [22], which is not surprising, as Cl − is too small to interact with both urea groups at the same time (Figures S22 and S23).For H 2 PO 4 − , the broadening of both NH signals over the course of the titration and the complex behavior of other signals prevented quantification of the interaction of 13 with H 2 PO 4 − (Figure S21).However, the sharpening of the signals with around 1.8 equivalents of H 2 PO 4 − could indicate that 13 can bind two H 2 PO 4 − anions.Molecular modeling confirmed that a H 2 PO 4 − dimer [23] would fit in the binding site of 13 (Figure S24).
Cl − , the changes in chemical shifts could be fitted to a 1:2 binding model (Figure S20), which gave affinity constants Ka(1:1)= 87 M −1 and a Ka(1:2) = 17 M −1 .This Ka(1:1) value is similar to the one obtained for a simple urea with bis(CF3)phenyl groups [22], which is not surprising, as Cl − is too small to interact with both urea groups at the same time (Figures S22  and S23).For H2PO4 − , the broadening of both NH signals over the course of the titration and the complex behavior of other signals prevented quantification of the interaction of 13 with H2PO4 − (Figure S21).However, the sharpening of the signals with around 1.8 equivalents of H2PO4 − could indicate that 13 can bind two H2PO4 − anions.Molecular modeling confirmed that a H2PO4 − dimer [23] would fit in the binding site of 13 (Figure S24).

Materials and Methods
All chemicals used were bought through either Sigma Aldrich, VWR, or Fluorochem and were used as received unless otherwise stated.Dry solvents were either bought as anhydrous solvents through commercial suppliers or dried over molecular sieves for at least 48 h before use.Column chromatography was carried out using silica gel with a 60 Å pore size and a particle size of 40-63 µm for flash column chromatography (FCC).TLC analysis was carried out on aluminum-backed silica gel plates visualized by a UV lamp or, when appropriate, permanganate or ninhydrin stains.Melting points were measured on a Cole-Parmer Stuart SMP-200.IR spectroscopy was carried out on a Shimadzu IR-Spirit-X FTIR spectrometer.NMR analysis was carried out on either a 400 MHz Varian spectrometer, a 400 MHz Jeol spectrometer, or a 600 MHz Jeol spectrometer.Chemical shifts are reported in ppm relative to the residual solvent signal of the deuterated solvent and the coupling constants are reported in Hz.ESI-MS Mass spectrometry was carried out on a Thermo Finnigan LCQ-DecaXP and HRMS was carried out on an Agilent QTOF 6520 by electron spray ionization.

Synthesis of 2-Iodo-4-nitrobenzoic Acid (6)
Following previously reported large-scale synthesis of this compound [20].To a stirred solution of 2-amino-4-nitrobenzoic acid (5.0 g, 27.45 mmol) in water (80 mL) cooled on an ice bath was added concentrated sulfuric acid (5.55 mL) and a solution of sodium nitrite (2.0 g, 29.65 mmol) in water (14 mL), added dropwise while keeping the temperature less than 5 °C.The mixture was stirred at 0-5 °C for 2 h, and then a solution of potassium iodide (6.290 g, 37.88 mmol in water (14 mL) was added over 10 min maintaining the temperature below 5 °C.The mixture was heated to 100 °C for 2 h and then cooled in an ice bath.The formed precipitate was collected by filtration, taken up in ether (55 mL), and washed with 20% aqueous thiosulfate solution (3 × 20 mL).The organic layer was dried with MgSO4 and evaporated to dryness under reduced pressure.The solid residue was

Materials and Methods
All chemicals used were bought through either Sigma Aldrich, VWR, or Fluorochem and were used as received unless otherwise stated.Dry solvents were either bought as anhydrous solvents through commercial suppliers or dried over molecular sieves for at least 48 h before use.Column chromatography was carried out using silica gel with a 60 Å pore size and a particle size of 40-63 µm for flash column chromatography (FCC).TLC analysis was carried out on aluminum-backed silica gel plates visualized by a UV lamp or, when appropriate, permanganate or ninhydrin stains.Melting points were measured on a Cole-Parmer Stuart SMP-200.IR spectroscopy was carried out on a Shimadzu IRSpirit-X FTIR spectrometer.NMR analysis was carried out on either a 400 MHz Varian spectrometer, a 400 MHz Jeol spectrometer, or a 600 MHz Jeol spectrometer.Chemical shifts are reported in ppm relative to the residual solvent signal of the deuterated solvent and the coupling constants are reported in Hz.ESI-MS Mass spectrometry was carried out on a Thermo Finnigan LCQ-DecaXP and HRMS was carried out on an Agilent QTOF 6520 by electron spray ionization.

Synthesis of (2-Iodo-4-nitrophenyl)methanol (7)
Following the general procedure reported in [21].A flame-dried round bottom flask under an argon atmosphere was charged with 2-iodo-3-methylbenzoic acid (1 g, 3.41 mmol), THF (22.0 mL), and NaBH 4 (0.516 g, 13.65 mmol) and the resulting solution cooled to 0 • C. A solution of I 2 (0.866 g, 3.41 mmol) in THF (11.0 mL) was added dropwise to the reaction mixture at 0 • C and the reaction mixture was stirred at room temperature for 17 h.Aqueous HCl (1 M, 72.5 mL) was added to the reaction mixture and the aqueous phase was extracted with Et 2 O three times.The combined organic layer was washed with brine, dried over Na 2 SO 4 , filtrated, and concentrated.The obtained residue was purified by FCC (1:1 ethyl acetate/heptane) to yield the desired compound as an orange solid (828 mg, 87%).Mp: 94-96 • C (lit.96.4-97.5 • C [21]). 1  Following the general procedure reported in [21].A mixture of (2-iodo-4-nitrophenyl)methanol 7 (200 mg, 716 µmol) and MnO 2 (1.93 g, 22.15 mmol) in CH 2 Cl 2 (10.0 mL) was refluxed for 14 h.After cooling to rt, the mixture was filtered over a celite pad, the pad washed with additional CH 2 Cl 2 (3 × 10.0 mL), and the solvent removed under reduced pressure to give the desired aldehyde (140 mg, 70%).Mp: 82-85 • C (lit.84.5 • C [19]). 1 H NMR (400 MHz, chloroform-d) δ 10.14 (d, J = 0.78, 1H), 8.79 (d, J = 2.1, 1H), 8.3 (ddd, J = 8.36, 2.2, 0.78 1H), 8.01 (d, J = 8.5, 1H). 13  Based on the procedure published by Bacon and Lindsay [19], 2-iodo-4-nitrobenzaldehyde 8 (130 mg, 469 µmol) was mixed together with copper powder (89 mg, 1.41 mmol) and DMF (10 mL) in a flame-dried three-necked round bottom flask fitted with a reflux condenser, a nitrogen balloon, and a vacuum outlet.Three vac/nitrogen cycles were performed, and the mixture heated to reflux for 2 h.After cooling to room temperature, the mixture was filtered through a pad of celite, and the pad washed with CH 2 Cl 2 (2 × 15 mL).The combined organic phase was evaporated in vacuo to yield the biphenyl compound as a light-brown solid, which was used for the next step without further purification (66 mg, 95%).Mp: 201-203 Based on the procedure published by Bacon and Lindsay [19], 5,5 ′ -dinitro-[1,1 ′biphenyl]-2,2 ′ -dicarbaldehyde 9 (351 mg, 1.17 mmol) was dissolved in glacial acetic acid (3 mL) and heated to reflux.Hydrazine hydrate (116 µL, 2.34 mmol) in glacial acetic acid (1 mL) was added dropwise, and the mixture heated for a further 4 h.The mixture was cooled to room temperature and left in a fridge overnight to precipitate.The following morning, the formed precipitate was filtered and the mother liquor concentrated to roughly 1 mL and left in the fridge for one additional day to precipitate further.The resulting precipitate was filtered off, and the combined filtrides dried in vacuo to yield the desired compound as brown-colored needle-like crystals (226 mg, 72%).Mp: Decomposition above 260 • C (lit.282-282.5 [19]).FT-IR (cm −1 ): 1340, 1502, 1508. 1  3,6-dinitrophenanthrene 10 (60 mg, 223 µmol) was suspended in ethanol (3 mL) and water (0.8 mL).Iron powder (86 mg, 1.54 mmol) and ammonium chloride (60 mg, 1.12 mmol) were added and the mixture was kept at reflux for 12 h.The reaction was then filtered through a pad of celite and the pad washed with H 2 O/EtOH (20%, 5 mL) and ethyl acetate (5 mL).The filtrate was then diluted with additional ethyl acetate (10 mL) and brine (10 mL).The two phases were separated and the aqueous phase was extracted twice more with ethyl acetate (10 mL).The combined organic phase was washed with saturated Na 2 CO 3 , water, and brine and dried with MgSO 4 to yield the crude diamine as a brown solid (33 mg, 71%).FT-IR (cm −1 ): 1621, 1655, 3337. 1 A flame-dried 5 mL round bottom flask fitted with a septum with an argon balloon was vented to the atmosphere to replace the air with argon.3,6-diaminophenanthrene 11 (26 mg, 124 µmol) was transferred to the flask as a CH 2 Cl 2 (4 mL) solution using dry CH 2 Cl 2 .Next, the 3,5-bis(trifluoromethyl)phenyl isocyanate (43 µL, 249 µmol) was added dropwise over 2 min, and the resulting mixture left to stir for two hours.The resulting precipitate was filtered and washed with cold CH 2 Cl 2 (3 × 3 mL) and dried in vacuo to yield the expected bis-urea as a tan solid (53 mg, 59%) Mp: above 300 3.8.General Procedure for 1 H-NMR Titrations 1 H-NMR titrations: All titrations were carried out at a 1 mM host concentration in DMSO-d6 with 0.5% H 2 O. Stock solutions of guests were prepared using the host solution to ensure that no dilution of the host took place over the course of the titration.TBA salts were dried under a high vacuum prior to the preparation of stock solutions with concentrations in the range of 20-200 mM depending on the titration.Aliquots of guest stock solution were added sequentially to an NMR tube with the free host and a proton spectrum acquired for each addition on a 400 MHz Varian spectrometer.For the chloride titration, the chemical shift of NH protons and other relevant signals were fitted to a 1:2 binding model using Bindfit [24-26].

Molecular Modeling
Modeling was performed by Monte Carlo multiple minimum (MCMM) conformational searches (100 steps per torsion angle, maximum 1000 steps in total), with an energy window of 21 kJ/mol performed in Schrödinger Release 2019-1 using MacroModel in Maestro (version 11.9.011).The force field used is OPLS3e, OPLS_2005, or MMFF, and no implicit solvation.The structures shown are the lowest energy conformations obtained from the calculations.

Conclusions
In this work, we have presented the facile synthesis of the novel anion receptor 1,1 ′ -(phenanthrene-3,6-diyl)bis(3-(3,5-bis(trifluoromethyl)phenyl)urea) 13 and shown that 13 interacts with anions of different sizes.The synthetic route presented consists of seven synthetic steps using readily available starting materials and reagents, with only a single step requiring chromatographic separation.Full characterization by 1 H and 13 C NMR, IR spectroscopy, and mass spectrometry of the intermediates are reported here, which were absent in the previous reports of 3,6-dinitrophenanthrene [19] and 3,6-diaminophenanthrene [18].In addition, the facile synthetic route to the 3,6-diaminophenanthrene (11) scaffold reported here will lower the barrier for its use in new supramolecular receptors, optoelectronic materials, or other applications.In these applications, the fluorescence of phenanthrene building block 11 and its derivatives could be exploited as well.

Figure 1 .
Figure 1.Examples of reported aromatic diamine scaffolds, for which the anion binding site upon formation of (thio)ureas increases from left to right (1-4), as well as the aromatic diamine scaffold that is used in this work(11).

Figure 2 .
Figure 2. Molecular model of a phenanthrene bis-urea in the presence of H2PO4 − showing good interaction between both urea groups and the phosphate guest.

Figure 1 .
Figure 1.Examples of reported aromatic diamine scaffolds, for which the anion binding site upon formation of (thio)ureas increases from left to right (1-4), as well as the aromatic diamine scaffold that is used in this work(11).

Figure 1 .
Figure 1.Examples of reported aromatic diamine scaffolds, for which the anion binding site upon formation of (thio)ureas increases from left to right (1-4), as well as the aromatic diamine scaffold that is used in this work(11).

Figure 2 .
Figure 2. Molecular model of a phenanthrene bis-urea in the presence of H2PO4 − showing good interaction between both urea groups and the phosphate guest.

Figure 2 .
Figure 2. Molecular model of a phenanthrene bis-urea in the presence of H 2 PO 4 − showing good

Scheme 2 .
Scheme 2. Synthetic scheme of the reduction of 3,6-dinitrophenanthrene 10 and the subsequent reaction of the resulting 3,6-diaminophenanthrene 11 with isocyanate 12 to yield the final receptor 13.

Scheme 2 .
Scheme 2. Synthetic scheme of the reduction of 3,6-dinitrophenanthrene 10 and the subsequent reaction of the resulting 3,6-diaminophenanthrene 11 with isocyanate 12 to yield the final receptor 13.

Scheme 2 .
Scheme 2. Synthetic scheme of the reduction of 3,6-dinitrophenanthrene 10 and the subsequent reaction of the resulting 3,6-diaminophenanthrene 11 with isocyanate 12 to yield the final receptor 13.