Functional Characterization of the Ryanodine Receptor Gene in Diaphorina citri
Abstract
:1. Introduction
2. Materials and Methods
2.1. Insects
2.2. Full-Length Cloning of DcRyR cDNA
2.3. Sequencing and Phylogenetic Analysis
2.4. Detection of Alternative Splice (AS) Sites
2.5. Detection of RyR Mutations in D. citri Populations
2.6. Data Analysis of DcRyR Expression
3. Results
3.1. Isolation of DcRyR
3.2. Conserved Domain and Phylogenetic Analysis of DcRyR
3.3. Detection of AS Sites of DcRyR
3.4. DcRyR Expression Profiles
3.5. Detection of DcRyR Mutations
4. Discussion
4.1. Isolated and Characterization of DcRyR
4.2. AS Site of DcRyR
4.3. DcRyR Expression Profiles
4.4. Detection of DcRyR Mutations
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Liu, T.-S.; Sun, X.-L.; Bin, M.-L.; Yi, G.-J.; Zhang, X.-X. Functional Characterization of the Ryanodine Receptor Gene in Diaphorina citri. Life 2022, 12, 2005. https://doi.org/10.3390/life12122005
Liu T-S, Sun X-L, Bin M-L, Yi G-J, Zhang X-X. Functional Characterization of the Ryanodine Receptor Gene in Diaphorina citri. Life. 2022; 12(12):2005. https://doi.org/10.3390/life12122005
Chicago/Turabian StyleLiu, Tian-Sheng, Xue-Li Sun, Min-Liang Bin, Gan-Jun Yi, and Xin-Xin Zhang. 2022. "Functional Characterization of the Ryanodine Receptor Gene in Diaphorina citri" Life 12, no. 12: 2005. https://doi.org/10.3390/life12122005