Small Bowel—Key Player in Health and Disease

Over the last two decades, remarkable progress has been made in understanding the etiology and pathophysiology of diseases [...].

pathobionts in the process of intestinal barrier alterations, which were followed by their translocation to lymph nodes and the hepatic portal system, triggering systemic lupus erythematosus (SLE) [5]. Thaiss et al. reported that hyperglycemia leads to disruption of the intestinal barrier followed by intestinal inflammation and systemic infection [6]. Spadoni et al. recently documented that the presence of the gut-vascular barrier (GVB) in the small intestine controls the dissemination of bacteria into the bloodstream [7]. The authors reported a decrease of the wnt/beta catenin-inducible gene Axin2 (a marker of stem cell renewal) in gut endothelium under the presence of Salmonella typhimirum in the SB. The GVB was modified in patients with coeliac disease (CD) with altered serum transaminases, which suggests that GVB deterioration may be responsible for liver damage in CD patients [7]. It has been shown that disruption of epithelial and vascular barriers in the intestine were early events in non-alcoholic steatohepatitis (NASH), and GVB leakage marker could be identified in colonic biopsies in patients with NASH [8]. Of importance, SB epithelial and vascular barriers are FXR-controlled, which opens avenues to clinical trials aimed at investigation of novel FXR-agonists as future therapeutics [9]. Of interest, intestinal barriers could be monitored in vivo with the aid of confocal laser endomicroscopy (CLE) [10,11].
Therefore, the SB is considered a key player in metabolic disease development [12], including diabetes mellitus and NASH, and other diet-related disorders such as coeliac and non-coeliac enteropathies. Another major field is drug metabolism and its interaction with small bowel microbiome [13]. Moreover, the emergence of gut-brain, gut-liver, and gut-blood barriers point towards the important role of the SB in the pathogenesis of previously unthought and GI-unrelated conditions such as neurodegenerative and cardiovascular disease [14,15]. The SB remains an organ that is difficult to fully access and assess and accurate diagnosis often poses a clinical challenge. Undoubtedly, the therapeutic potential remains untapped. Therefore, it is now time to direct more of our interest towards the SB and unravel the interplay between the SB and other GI and non-GI related diseases.
In this Journal of Clinical Medicine Special Issue, "Diagnosis and Treatment of Small Bowel Disorders", several groups of investigators contributed their knowledge to the field of SB research by presenting original papers and reviews.
Enaud et al. [16] describe original observations by utilizing next-generation sequencing (NGS), that intestinal inflammation in children with Cystic Fibrosis (CF) was associated with alterations of microbiota similar to those observed in Crohn's disease (CrDs). Authors for the first time applied novel CrDs Microbial-Dysbiosis index in CF patients and pointed towards the importance of gut-lung axis in CF prognosis [16]. Of interest, intestinal inflammation was associated with previous intravenous antibiotic courses for CF [13]. This observation is important as global awareness of antibiotic resistance rises. Nakamura et al. [17] sought to evaluate the validity of using capsule endoscopy (CE) to monitor the effect of medical treatment on SB mucosal healing in post-operative CrDs patients, regardless of the presence of clinical symptoms. Although the significance of endoscopic monitoring has been widely accepted in CrDs, to date, only a few studies looked at the validity and effectiveness of escalating treatment for patients in clinical remission but with endoscopically visible active mucosal lesion. The authors demonstrated that CrDs patients in clinical remission with ongoing intestinal inflammation at the time of the CE could benefit from additional treatment. This study motivates physicians to optimize treatment plans for asymptomatic CD patients.
Several characteristics of SB lesions (e.g., mucosal disruption, bleeding, irregular surface, polypoid appearance, color, delayed passage, white villi, and invagination) have been described to better predict SB lesions, such as intestinal bulges, masses, and tumors. The lack of precise features in characterizing these lesions places a limitation on the accuracy of CE diagnosis. Therefore, Min et al. [18] in their retrospective study, evaluated the utility of an additional morphologic criterion, the mucosal protrusion angle (MPA), which was defined as the angle between a SB protruding lesion and its surrounding mucosa. The authors documented that MPA was a simple and useful tool for differentiating between intestinal true masses and non-significant bulges [18]. Their observation creates a useful extra tool for those who are faced with the question of 'mass or bulge?' SB microbiota alterations have also been implicated in the pathogenesis of surgical site infections (SSIs) and surgery-related complications (SRCs). Skonieczna-Żydecka et al. [19] conducted a systematic review with meta-analysis and meta-regression of randomized clinical trials investigating the efficacy of probiotics and synbiotics to counteract SSIs and SRCs in patients under various surgical treatments. The authors aimed to determine the mechanisms behind probiotic/synbiotic action. Their meta-analysis revealed that probiotics/synbiotics administration prior and at a time of major abdominal surgery, leads to a reduction in the incidence of SSIs and SRCs (e.g., abdominal distension, diarrhea, pneumonia, sepsis, urinary tract infection, postoperative pyrexia). Furthermore, probiotics/synbiotics were associated with shortening of the duration of antibiotic therapy and hospital stay. Based on current evidence, the action of probiotics/synbiotics in surgical patients seems to be exerted via modulation of gut-immune response and production of short-chain fatty acids (SCFAs) [19].
Included also in this special issue, Singh et al. [20] comprehensively reviewed the pros and cons of biomarkers in coeliac disease and summarized the current status of coeliac disease screening, diagnosis, and monitoring. The review could guide clinicians in diagnosis and monitoring of patients with coeliac disease. As biomarkers allow for smart targeted-screening, similarly imaging spectroscopy (a combination of digital imaging and spectroscopy, also known as hyperspectral/multispectral (HS/MS) imaging (HSI/MSI) technology) allows for smart tissue visualization beyond the limitations of the human eye. HSI has been utilized for various research purposes including: (i) food quality inspection, (ii) optimization of the recycling process, (iii) art painting renovations, (iv) geology and minerals inspection, (v) soil evaluation and (vi) plant response to stress. HSI has also been evolving in the field of medical research in gastroenterology, pathology, and surgery. This modality was used to generate alternative visualization of tissues, abdominal organ differentiation, identification of surgical site resection, and abdominal ischemia to name a few. Ortega et al. [21] in their thorough review provided a detailed summary of the most relevant research work in the field of gastroenterology using HSI.
Last but not least, Skonieczna-Żydecka et al. [22] in a narrative review published in this issue of Journal of Clinical Medicine, discussed involvements of GBA deregulation in the origin of brain-gut disorders. The authors hypothesized that stem cell-host microbiome cross-talk was potentially involved in GBA disorders. Interestingly, patients with inflammatory bowel disease (IBD) have an elevated risk of mental illness, and depression increases the risk of IBD. Of key interest, are observations that multiple drugs are known to induce metabolic malfunctions, possibly through alterations of SB milieu. These alterations result in body weight gain, metabolic disturbances, and suppression of metabolic resting rate. The authors in their comprehensive review presented the current state of the art knowledge of the role of GBA in GI and psychiatric comorbidities. The current evidence supports the notion that an injury to the intestinal mucosa can result in significant, though delayed, metabolic consequences that may seriously affect the health of an individual. Future investigations [23] into the pathophysiology of host-microbe interactions should focus on the small bowel [24,25], which is still relatively inaccessible. Therefore, the research is challenging but necessary to pave the way to new findings and solutions in the clinical area of the small bowel [26] and beyond [27].
Dr Marlicz and Dr Koulaouzidis are Guest Editors of this Special Issue of the Journal of Clinical Medicine (ISSN 2077-03830), which belongs to the journal's section Gastroenterology and Hepato-Pancreato-Biliary Medicine.

Conflicts of Interest:
The authors declare that there is no conflict of interest.