Genome-Wide Identiﬁcation and Analysis of OsSPXs Revealed Its Genetic Inﬂuence on Cold Tolerance of Dongxiang Wild Rice (DXWR)

: SPX-domain proteins (small proteins with only the SPX domain) have been proven to be involved in phosphate-related signal transduction and regulation pathways. Except for OsSPX1 research showing that it plays a role in the process of rice adaptation to cold stress, the potential functions of other SPX genes in cold stress are unknown. Therefore, in this study, we identiﬁed six OsSPXs from the whole genome of DXWR. The phylogeny of OsSPXs has a strong correlation with its motif. Transcriptome data analysis showed that OsSPXs were highly sensitive to cold stress, and real-time PCR veriﬁed that the levels of OsSPX1 , OsSPX2 , OsSPX4 , and OsSPX6 in cold-tolerant materials (DXWR) during cold treatment were higher than that of cold-sensitive rice (GZX49). The promoter region of DXWR OsSPXs contains a large number of cis-acting elements related to abiotic stress tolerance and plant hormone response. At the same time, these genes have expression patterns that are highly similar to cold-tolerance genes. This study provides useful information about OsSPXs , which is helpful for the gene-function research of DXWR and genetic improvements during breeding.


Introduction
Phosphorus (P) is essential for plant growth and development, and inorganic phosphate (Pi) is a form of phosphorus available to plants [1][2][3]. SPX-domain proteins are widely found in eukaryotes, including animals, plants, and fungi. This domain is named according to the first letter of the three proteins Syg1, Pho81, and XPRI [4,5]. SPX-domain proteins have been proven to participate in phosphate-related signal transduction and regulation pathways. In plants, SPX has four subfamilies, namely, SPX-EXS (named after Saccharomyces cerevisiae, Erd1; mammalian, Xpr1; and Saccharomyces cerevisiae, Syg1), SPX-MFS (a major promoter superfamily), SPX ring (a really interesting new gene), and SPXs (small proteins of the SPX domain) [6][7][8]. In Arabidopsis, SPX-EXS proteins (such as AtPHO1) are involved in transferring Pi from roots to buds [9]. The pho1 mutant is characterized by a serious lack of Pi in stems, but the content of Pi in roots is normal [9,10]. In addition, three members of the AtPHO1 family showed possible interactions with signaling pathways involved in Pi deficiency and responses to auxin, cytokinin, and abscisic acid (ABA) [11][12][13]. AtVPT1 and OsSPX-MFS1, belonging to SPX-MFS, can transfer Pi from the cytoplasm to vacuoles [14,15]. For proteins with only the SPX domain, four members were identified in Arabidopsis thaliana (AtSPX1-AtSPX4) and six members

Phylogenetic Tree Analysis and Motif Analysis of OsSPXs in DXWR
The protein data of DXWR OsSPXs (named OsSPX1~6 according to each gene's corresponding gene in Nipponbare) were obtained through the ORYZA GENOME database (online: http://www.ricegermplasmgenome.com/, accessed on 26 March 2022). The OsSPXs development evolution tree was constructed through MEGA, and the online MEME server was used for motif analysis. OsSPX1 has the highest homology with OsSPX2; OsSPX3 has a close genetic relationship with OsSPX5, followed by OsSPX6; and OsSPX4 has a distance genetic relationship with other OsSPXs (Figure 1a). In combination with the motif analysis, motifs 1~5 exist in all OsSPXs (Figure 1b). Motifs 6 and 10 exist in OsSPX3 and OsSPX6, motif 7 is unique to OsSPX1 and OsSPX2, motif 8 only exists in OsSPX3 and OsSPX5, and motif 9 exists in all OsSPXs except OsSPX4 and OsSPX6 (Figure 1c).

Gene Collinearity Analysis of OsSPXs
Gene replication events are crucial to the evolution of gene families and often play an important role in gene amplification and the generation of new functional genes. Therefore, we have studied the repeat events of OsSPXs in the whole genome of DXWR and found that OsSPXs have no tandem repeat events. However, using BLASTP and MCScanX methods, three pairs of fragment-duplication events were found in OsSPXs (Figure 2a), and these fragment-duplication genes were distributed on chromosomes 2, 3, 6, 7, and 10. These results suggest that fragment-replication events play an important role in the amplification of OsSPXs.
We constructed a collinearity map of three gramineous plants (maize, DXWR, and wheat) (Figure 2b). Among the homologous OsSPXs of DXWR and other species, there were five pairs of genes homologous with maize and four pairs with wheat. Interestingly, OsSPX1, 4, and 5 all have homologous genes in the three species. Due to polyploidization, some genes differentiate during evolution and generate new gene functions to adapt to the environment. The number of genes with collinear relationships in wheat is three times that of rice (OsSPX1, 4, 5, and 6), indicating that hexaploid wheat with these genes existed before differentiation and played an important role in these wheat after differentiation.

Gene Collinearity Analysis of OsSPXs
Gene replication events are crucial to the evolution of gene families and often play an important role in gene amplification and the generation of new functional genes. Therefore, we have studied the repeat events of OsSPXs in the whole genome of DXWR and found that OsSPXs have no tandem repeat events. However, using BLASTP and MCScanX methods, three pairs of fragment-duplication events were found in OsSPXs (Figure 2a), and these fragment-duplication genes were distributed on chromosomes 2, 3, 6, 7, and 10. These results suggest that fragment-replication events play an important role in the amplification of OsSPXs.
We constructed a collinearity map of three gramineous plants (maize, DXWR, and wheat) (Figure 2b). Among the homologous OsSPXs of DXWR and other species, there were five pairs of genes homologous with maize and four pairs with wheat. Interestingly, OsSPX1, 4, and 5 all have homologous genes in the three species. Due to polyploidization, some genes differentiate during evolution and generate new gene functions to adapt to the environment. The number of genes with collinear relationships in wheat is three times that of rice (OsSPX1, 4, 5, and 6), indicating that hexaploid wheat with these genes existed before differentiation and played an important role in these wheat after differentiation.

Expression Patterns of OsSPXs in Different Rice Tissues and Their Responses to Cold Stress
The analysis of gene expression patterns can provide important information for identifying the biological functions of genes of interest. In order to study the expression pattern of OsSPXs, the expression data of OsSPXs stored in the public database were used to carry out such analyses on twelve tissues/organs of rice, including seeding root, young leaf, SAM, young inflorescence, inflorescence P2-P6, and seed S1-S5. As shown in Figure 3a, the expression spectrum thermogram shows that OsSPX1, 2, and 4 are highly expressed at different growth stages. Other OsSPXs have tissue-specific expression patterns. For example, OsSPX5 is only highly expressed in the seeding root. In addition, OsSPX3 showed specific high expression at the late stage of seed development. However, OsSPX6 only showed higher expression levels in mature leaves. These expression analyses showed that OsSPX1, 2, and 4 genes may play a constitutive function in the process of plant growth and development, and that some genes, such as OsSPX3, 5, and 6, may play different roles in different tissues/organs and different seed-development stages. We used the transcriptome data of Dongxiang wild rice before and after treatment at 4 • C to further analyze the expression levels of OsSPXs under low-temperature stress. The results showed that, with the exception of OsSPX3 and OsSPX5, the expression levels of OsSPXs did not change significantly after 4 h of treatment compared to 0 h of treatment; however, they increased more than two times after 12 h of treatment ( Figure 3b). These OsSPXs may be involved in the cold-tolerance process of DXWR.

Expression Patterns of OsSPXs in Different Rice Tissues and Their Responses to Cold Stress
The analysis of gene expression patterns can provide important information for identifying the biological functions of genes of interest. In order to study the expression pattern of OsSPXs, the expression data of OsSPXs stored in the public database were used to carry out such analyses on twelve tissues/organs of rice, including seeding root, young leaf, SAM, young inflorescence, inflorescence P2-P6, and seed S1-S5. As shown in Figure  3a, the expression spectrum thermogram shows that OsSPX1, 2, and 4 are highly expressed at different growth stages. Other OsSPXs have tissue-specific expression patterns. For example, OsSPX5 is only highly expressed in the seeding root. In addition, OsSPX3 showed specific high expression at the late stage of seed development. However, OsSPX6 only showed higher expression levels in mature leaves. These expression analyses showed that OsSPX1, 2, and 4 genes may play a constitutive function in the process of plant growth and development, and that some genes, such as OsSPX3, 5, and 6, may play different roles in different tissues/organs and different seed-development stages. We used the transcriptome data of Dongxiang wild rice before and after treatment at 4 °C to further analyze the expression levels of OsSPXs under low-temperature stress. The results showed that, with the exception of OsSPX3 and OsSPX5, the expression levels of OsSPXs did not change significantly after 4 h of treatment compared to 0 h of treatment; however, they increased more than two times after 12 h of treatment ( Figure 3b). These OsSPXs may be involved in the cold-tolerance process of DXWR.
DXWR and GZX49 were treated for 14 days at 4 °C and then recovered at room temperature for 7 d. We counted the living plants (containing about 40% green leaves) and dead plants of the two materials to determine the survival rates. The survival rate of DXWR was 90%, whereas that of GZX49 was only 10% (Figure 3c). We believe that DXWR is a cold-resistant material and that GZX49 is cold-sensitive to cold stress. Then, real-time PCR (qPCR) was used to check their expressions after cold-stress (4 °C) treatment in seed- DXWR and GZX49 were treated for 14 days at 4 • C and then recovered at room temperature for 7 d. We counted the living plants (containing about 40% green leaves) and dead plants of the two materials to determine the survival rates. The survival rate of DXWR was 90%, whereas that of GZX49 was only 10% (Figure 3c). We believe that DXWR is a cold-resistant material and that GZX49 is cold-sensitive to cold stress. Then, real-time PCR (qPCR) was used to check their expressions after cold-stress (4 • C) treatment in seedlings of cold-tolerant wild rice (DXWR) and cold-sensitive indica rice (GZX49). Time expression profiles of four OsSPXs were obtained in DXWR and GZX49 under low-temperature stress ( Figure 3d). In general, the four OsSPXs in both materials were induced due to cold stress. The relative expressions of the other three genes (OsSPX1, 2, and 6) reached their peaks at 24 h after treatment at 4 • C, with the exception of OsSPX4, which reached its maximum at 12 h after treatment at 4 • C. In addition, the expression levels of OsSPX1 and 2 in DXWR were significantly higher than in GZX49 at 24 h after treatment at 4 • C, and the relative expression levels of OsSPX4 and 6 in DXWR were significantly higher than in GZX49 after treatment at 4 • C at 4, 12, and 24 h. These results suggest that these four OsSPXs may play important roles in the rice response to cold stress. and the relative expression levels of OsSPX4 and 6 in DXWR were significantly higher than in GZX49 after treatment at 4 °C at 4, 12, and 24 h. These results suggest that these four OsSPXs may play important roles in the rice response to cold stress. The phenotypes of cold-tolerant rice and cold-sensitive rice after treatment at 4 °C and their survival rates. (d) The expression levels of OsSPX1, 2, 4, and 6 in GZX49 and DXWR after cold stress, detected using qPCR. The results were statistically analyzed using Student's t-test (* p < 0.05, , *** p < 0.005). Transcription levels relative to 0 h, which was set to 1, are presented as the mean and SE of triplicates. LOC_Os03g13170 (Ubiquitin) is the control gene.

Analysis of the Regulatory Elements of the OsSPX Promoter in DXWR and Their Correlations with Cold-Tolerance-Gene Expression
Cis-acting elements can be recognized from transcription factors and participate in the expression of tissue-specific and stress-response genes. In order to further study the roles and regulation mechanisms of OsSPXs in various biological processes, especially the response to cold stress, PLANTCARE and PlantTFDB were used to identify 2000 bp upstream sequences of four OsSPXs of DXWR. A total of 42 cis-acting elements were detected in PLANTCARE, of which 8 stress-response elements were the most, followed by lightresponse elements, growth-and development-related elements, and MYB transcriptionfactor-binding elements (Figure 4a). In general, OsSPXs are more likely to regulate stress responses, and the original elements of stress response are ARE, ABRE, STRE, WUN-motif, GC-motif, ABRE3a, LTR, and box-S. Hormone reaction elements include abscisic acid The expression levels of OsSPX1, 2, 4, and 6 in GZX49 and DXWR after cold stress, detected using qPCR. The results were statistically analyzed using Student's t-test (* p < 0.05, *** p < 0.005). Transcription levels relative to 0 h, which was set to 1, are presented as the mean and SE of triplicates. LOC_Os03g13170 (Ubiquitin) is the control gene.

Analysis of the Regulatory Elements of the OsSPX Promoter in DXWR and Their Correlations with Cold-Tolerance-Gene Expression
Cis-acting elements can be recognized from transcription factors and participate in the expression of tissue-specific and stress-response genes. In order to further study the roles and regulation mechanisms of OsSPXs in various biological processes, especially the response to cold stress, PLANTCARE and PlantTFDB were used to identify 2000 bp upstream sequences of four OsSPXs of DXWR. A total of 42 cis-acting elements were detected in PLANTCARE, of which 8 stress-response elements were the most, followed by lightresponse elements, growth-and development-related elements, and MYB transcriptionfactor-binding elements (Figure 4a). In general, OsSPXs are more likely to regulate stress responses, and the original elements of stress response are ARE, ABRE, STRE, WUN-motif, GC-motif, ABRE3a, LTR, and box-S. Hormone reaction elements include abscisic acid (AAGAA motif, AT-rich sequence, and ABRE4), gibberellin (GARE motif, GA motif, P-box, and TATC box), salicylic acid (TCA element), ethylene (ERE), and jasmonic acid (TGTCA motif and TGACG motif). In addition, the original elements of MYB, b-ZIP, and WRKY transcription-factor binding were also found in SPX. At the same time, 90 transcriptionfactor binding sites were identified with PlantTFDB, including ERF (22/90), WRKY (19/90), G2-like (10/90), Dof (9/90), NAC (4/90), MYB (4/90), and C2H2 (4/90) (Figure 4b). The diversity and function of these cis-acting elements and transcription-factor binding sites provide a deeper understanding of the biological functions of OsSPXs.

Discussion
The SPX subfamily only contains the SPX domain. It has been widely studied because of its role in plant Pi signal transduction. Rice is one of the most important food crops in the world. Studying the genes in the process of stress resistance is of great significance for stabilizing rice yield. However, at present, members of the SPX subfamily, as regulatory proteins in the process of adversity, have received little attention. This study identified six OsSPXs from DXWR and tried to explore their roles in cold stress.
Similar protein structures may indicate that genes have similar functions [39,40]. By comparing the homology of proteins, we found that OsSPX1 and OsSPX2 are closely related, and OsSPX3 has high homology with OsSPX5 and OsSPX6, whereas OsSPX4 is distantly related to other OsSPXs. These findings were further confirmed by analyzing the motif composition of OsSPXs. OsSPX1 and OsSPX2 contain specific motif 7, whereas

Discussion
The SPX subfamily only contains the SPX domain. It has been widely studied because of its role in plant Pi signal transduction. Rice is one of the most important food crops in the world. Studying the genes in the process of stress resistance is of great significance for stabilizing rice yield. However, at present, members of the SPX subfamily, as regulatory proteins in the process of adversity, have received little attention. This study identified six OsSPXs from DXWR and tried to explore their roles in cold stress.
Similar protein structures may indicate that genes have similar functions [39,40]. By comparing the homology of proteins, we found that OsSPX1 and OsSPX2 are closely related, and OsSPX3 has high homology with OsSPX5 and OsSPX6, whereas OsSPX4 is distantly related to other OsSPXs. These findings were further confirmed by analyzing the motif composition of OsSPXs. OsSPX1 and OsSPX2 contain specific motif 7, whereas OsSPX3 and OsSPX5 have specific motif 8. OsSPX3 and OsSPX6 have conservative motifs 7 and 10. OsSPX4 does not contain motifs 6-10. The subsequent intragenomic collinearity analysis showed that OsSPX1, OsSPX2, OsSPX3, OsSPX5, and OsSPX6 were derived from the replication of genomic fragments, which may come from the amplification of the whole genome. Homologous genes are highly conserved among species. Because they have similar important functions during evolution, they may be preserved, which has been found to be the case in gramineous crops [41]. Analysis of the collinearity between genomes revealed that OsSPX1, 4, and 5 contained direct homologous genes in maize, DXWR, and wheat. Except for OsSPX6, other OsSPXs have homologous genes in the maize genome. Three maize SPX proteins (ZmSPX3, 5, and 6) can interact with ZmPHR1, with the exception that ZmSPX5 and 6 can be induced by low phosphorus. The homologous genes of ZmSPX6, 5, and 3 in DXWR are OsSPX1, 2, and 4, respectively. Interestingly, OsSPX1, 2, and 4 can also adjust the phosphate balance by combining their SPX domain with OsPHR2 [42,43]. This shows that SPX has a highly conservative function in maize and rice. Common wheat (AABBDD) underwent two crosses and genome doubling from diploid ancestors to form heterohexaploid crops. In theory, one rice gene should correspond to three homologous wheat genes [44,45]. For example, the homologous genes of OsSPX1 in wheat, Traescs7a02g376200, Traescs7b02g277700, and Traescs7d02g372600, were all induced due to Pi starvation. It is also interesting that Traescs7a02g376200 and Traescs7d02g372600, as candidate genes for saline-alkali tolerance, were significantly upregulated after salinealkali treatment [46]. However, we know that OsSPX1 responds to Pi starvation and plays a positive regulatory role in cold tolerance. It can be seen that OsSPX1 is highly conserved in rice and wheat and has similar functions in the process of evolution.
As a signal transduction protein, SPX plays a role in Pi signal transport. OsSPX1, 2, and 4 are highly expressed throughout the rice growth period, indicating that they are important for rice growth. OsSPX1 has been proven to be related to the cold tolerance of rice. Overexpression of OsSPX1 can enhance the cold tolerance of transgenic rice and Arabidopsis. Through qPCR verification, four OsSPXs were significantly induced in the cold-tolerant material DXWR and the cold-sensitive material GZX49, which showed key roles in cold-tolerance processes. Importantly, the induction degrees in the cold-tolerant material DXWR of OsSPX1 and 2 at 4 • C for 24 h and of OsSPX4 and 6 at 4 • C for 4, 12, and 24 h, were significantly higher than that of the cold-sensitive material GZX49, and the expression levels increased with an increase in low-temperature treatment time. DXWR promoter analysis showed that OsSPX1, 2, 4, and 6 promoters contained cis-regulatory elements related to environmental stress and hormone induction, which may endow this gene with potential functions of responding to stress and regulating endogenous hormones. When plants are under external pressures, such as droughts, low or high temperatures, salt, and other stresses, these pressure signals activate corresponding transcription factors through a series of signals and combine with corresponding homeopathic regulatory elements, thus activating the expression of related genes to respond to the external signal stress. OsSPX1, 2, 4, and 6 all contain ARE, ABRE, MYB, jasmonic acid (TGTCA-motif and TGACG-motif), and AAGAA-motif, indicating that these genes may play a key role in improving the resistance of DXWR. In addition, we also identified a large number of stress-related transcription-factor binding sites: ERF, WRKY, G2-like, Dof, NAC, MYB, and C2H2. The existence of these transcription-factor binding sites confirmed the response of the OsSPX genes to stress. For example, OsSPX1 has 16 ERF binding sites and 5 WRKY.
Four OsSPXs and cold-tolerant genes have highly similar expression patterns under cold stress. For example, OsMYB3R-2 is highly correlated with OsSPX1 and OsSPX4 expressions. Previous studies have shown that OsMYB3R-2 is the main switch of tolerance to stress, which can improve the tolerance to stress by activating the expression of coldresponse genes such as OsDREB2A and O S DREB1 [47,48]. Interestingly, we also found MYB transcription-factor binding sites in the OsSPX1 and OsSPX4 promoter regions. To summarize, our study revealed the expression patterns of OsSPXs in different tissues and under cold stress, which highlighted the potential role of DXWR OsSPXs in coldstress tolerance.

Plant Materials and Evaluation of Cold Tolerance at the Seedling Stage
In this study, Dongxiang wild rice (DXWR) and indica rice (GZX49) were used as materials. These rice materials were planted in the rice base of the Rice Institute of the Jiangxi Academy of Agricultural Sciences. The harvested seeds were incubated at 40 • C for about 36 h to break dormancy, and then soaked in deionized water at 30 • C for about 60 h to germinate. A total of 30 germinated seeds were selected from each strain and planted in the soil in the pot for 12 h light (28 • C) and 12 h dark (26 • C) cycles, with a humidity of 80%. When most seedlings had grown to trefoil shape, the weak seedlings were removed, and the rice materials used for low-temperature stress were treated at 4 • C for 0, 4, 12, and 24 h. The collected samples were stored at −80 • C. The rest were treated in the incubator at a low temperature of 4 • C for 84 h, and then resumed growth at 28 • C for 7 days. After 14 days of treatment, the survival rate was determined by calculating the living plants (leaves containing about 40% of green parts) and dead plants. This process was repeated three times independently for the two materials.

Identification of SPX Gene in DXWR
The sequence information of DXWR comes from a gene-annotation website (http: //www.ricegermplasmgenome.com/, accessed on 26 March 2023), and a hidden Markov model containing the SPX-domain gene (PF03105) was used to search and screen the candidate gene in hmmer3 [49,50]. Then, it passed the NCBI conservative domain database (CDD; https://www.ncbi.nlm.nih.gov/cdd, accessed on 26 March 2023) and SMART database (http://smart.embl-heidelberg.de/, accessed on 12 March 2023) to confirm the integrity of the protein domain and that it only contained the SPX domain [51,52].

Analysis of Main Characteristics of SPX Gene in DXWR
Using MEGA11, the phylogenetic tree of DXWR was repeatedly constructed through neighbor connection (NJ), with guidance set to 1000 [53]. The distribution of all DUF26domain genes on the DXWR chromosome was analyzed and visualized using TBtools [54]. The characteristic motif of the SPX gene was determined with MEME (http://meme-suite. org/tools/meme, accessed on 12 March 2023). The base sequence number was 10, and the site distribution order of each sequence was either 0 or 1 [55].

Repetitive Events and Collinearity Analysis of OsSPXs
The multilinear analysis tool MCscanX analyzes the replication events of genes in the genome and uses it for visualization through Circos [56]. Genome data of wheat (Triticum_aesivum. IWGSC.52) and maize (Zea_mys. Zm-B73-REFERENCE-NAM-5.0.52) were acquired from the Ensembl plant genome website (https://plants.ensembl.org/index. html, accessed on 20 December 2022). The collinear relationships between DXWR and maize and wheat were analyzed and visualized using JCVI [57].

Promoter Analysis
According to the position of the gene on the chromosome, we extracted the DNA sequence 2 kb upstream of the promoter of the OsSPX gene coding region of Dongxiang wild rice. Using PlantCARE (http://bioinformatics.psb.ugent.be/webtools/plantcare/html/, accessed on 26 March 2022), the cis-acting element was predicted [58]. At the same time, the cis-acting element was submitted to the PlantTFDB database (http://planttfdb.gao-lab. org/, accessed on 26 March 2022). Transcription factor binding sites at 2 kb upstream of the candidate OsSPX promoter sequence were also predicted [59].

Correlation Analysis of the OsSPX Gene and Cold-Tolerance Genes
Correlation network analysis between the OsSPX gene and cold-tolerance genes was performed using OmicStudio tools (https://www.omicstudio.cn/tool accessed on 26 March 2023) (R version 3.6.3 igraph1.2.6).

Quantitative PCR
Total RNAs were isolated with a TRIzol kit (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions. The RNA was treated with DNase I (Invitrogen), and approximately 3 µg of total RNA was used to synthesize first-strand cDNA using oligo(dT) 18 as a primer (Promega, Shanghai, China). The trans-intron ACTIN primer (ACTIN-M) was used to detect whether the reverse-transcribed cDNA still had genomic DNA, and the cDNA without genomic DNA was used for subsequent qPCR. Quantitative PCR was performed using gene-specific primers and the FastStart Universal SYBR Green Master (Roche) on a qPCR ViiA7 system (Applied Biosystems). Genes ubiquitin (LOC_Os03g13170) and actin1 (LOC_Os03g50885), which were not differentially expressed in RNAseq, were used as the internal controls. The relative quantification method (2 −∆∆CT ) was used to evaluate gene expression levels [60]. As similar expression results were observed regardless of which control genes were used, the ubiquitin of expressions was applied for the relative expression analyses for every assayed sample. At least three biological replicates, each containing four that were technical, were performed for each experiment.

Conclusions
In this study, six OsSPXs were identified in the reference genome of DXWR, and the conserved domain, collinearity, expression pattern, and cis-elements were analyzed. Expression analysis showed that OsSPX1-6 had different expression levels in all tissues, indicating that these OsSPXs played an important role in rice growth and development. In addition, we also analyzed the expression of OsSPXs under low-temperature stress and found that OsSPX1, 2, 4, and 6 were strongly induced by low temperatures, and the expression level in DXWR was significantly higher than that in low-temperature-sensitive materials. Finally, the coexpression network of OsSPXs and cold-signal-related genes was constructed, indicating that OsSPXs may participate in the cold-tolerance process by participating in the known cold-stress-response gene pathway. In future research, these candidate genes should be knocked-out or over-expressed in rice for further functional verification. The excellent alleles of DXWR were polymerized into conventional rice through gene polymerization to enhance its cold tolerance under the condition of ensuring yield. In conclusion, our research results are valuable and will be useful for further explaining the biological function of OsSPXs and for genetic improvement.

Patents
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