Influence of Cucurbiturils on the Production of Reactive Oxygen Species by T- and B-Lymphocytes, Platelets and Red Blood Cells
Abstract
:1. Introduction
2. Results
3. Discussion
4. Materials and Methods
4.1. Materials, Reagents and Preparation
4.2. Isolation and Cultivation of Cells
4.2.1. Isolation and Cultivation of PBMC
4.2.2. Isolation and Cultivation of RBC
4.2.3. Isolation and Cultivation of Platelets
4.2.4. Cultivation of Tumor Lines
4.3. Treatment of Cells by Cucurbit[n]urils (n = 6, 7, 8)
4.4. Measurement of Intracellular Reactive Oxygen
4.5. Phenotype of Cells
4.6. Statistical Data Analysis
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Aktanova, A.A.; Boeva, O.S.; Barkovskaya, M.S.; Kovalenko, E.A.; Pashkina, E.A. Influence of Cucurbiturils on the Production of Reactive Oxygen Species by T- and B-Lymphocytes, Platelets and Red Blood Cells. Int. J. Mol. Sci. 2023, 24, 1441. https://doi.org/10.3390/ijms24021441
Aktanova AA, Boeva OS, Barkovskaya MS, Kovalenko EA, Pashkina EA. Influence of Cucurbiturils on the Production of Reactive Oxygen Species by T- and B-Lymphocytes, Platelets and Red Blood Cells. International Journal of Molecular Sciences. 2023; 24(2):1441. https://doi.org/10.3390/ijms24021441
Chicago/Turabian StyleAktanova, Alina A., Olga S. Boeva, Margarita Sh. Barkovskaya, Ekaterina A. Kovalenko, and Ekaterina A. Pashkina. 2023. "Influence of Cucurbiturils on the Production of Reactive Oxygen Species by T- and B-Lymphocytes, Platelets and Red Blood Cells" International Journal of Molecular Sciences 24, no. 2: 1441. https://doi.org/10.3390/ijms24021441