Effect of Lipopolysaccharide-Induced Inflammatory Challenge on β-Glucuronidase Activity and the Concentration of Quercetin and Its Metabolites in the Choroid Plexus, Blood Plasma and Cerebrospinal Fluid
Abstract
:1. Introduction
2. Results
2.1. Effect of LPS-Induced Acute Inflammation on Gene Expression and Activity of β-Glucuronidase in the ChP (Exp. 1)
2.2. Effect of LPS-Induced Acute Inflammation on β-Glucuronidase Activity and the Concentration of Q and Its Phase II Metabolites in Blood Plasma and Cerebrospinal Fluid (Exp. 2)
2.3. Effect of LPS on Gene Expression and Activity of β-Glucuronidase (Exp. 3) and Q3GA Accumulation in the Epithelial Cells of the Choroid Plexus In Vitro (Exp. 4)
3. Discussion
4. Materials and Methods
4.1. Animals and Experimental Design
4.2. Analytical Methods
4.2.1. Cortisol, Protein Level and β-Glucuronidase Activity
4.2.2. Cell Viability Assay (MTT) and Cytotoxicity Assay (LDH)
4.2.3. Western Blotting and CSF/Blood Plasma Quotient
4.2.4. Relative Gene Expression Assays
4.2.5. Analysis of Q and Its Metabolites with HPLC-MS/MS
4.3. Calculations and Statistical Analysis
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Domżalska, M.; Wiczkowski, W.; Szczepkowska, A.; Chojnowska, S.; Misztal, T.; Walter, F.R.; Deli, M.A.; Ishikawa, H.; Schroten, H.; Schwerk, C.; et al. Effect of Lipopolysaccharide-Induced Inflammatory Challenge on β-Glucuronidase Activity and the Concentration of Quercetin and Its Metabolites in the Choroid Plexus, Blood Plasma and Cerebrospinal Fluid. Int. J. Mol. Sci. 2021, 22, 7122. https://doi.org/10.3390/ijms22137122
Domżalska M, Wiczkowski W, Szczepkowska A, Chojnowska S, Misztal T, Walter FR, Deli MA, Ishikawa H, Schroten H, Schwerk C, et al. Effect of Lipopolysaccharide-Induced Inflammatory Challenge on β-Glucuronidase Activity and the Concentration of Quercetin and Its Metabolites in the Choroid Plexus, Blood Plasma and Cerebrospinal Fluid. International Journal of Molecular Sciences. 2021; 22(13):7122. https://doi.org/10.3390/ijms22137122
Chicago/Turabian StyleDomżalska, Małgorzata, Wiesław Wiczkowski, Aleksandra Szczepkowska, Sylwia Chojnowska, Tomasz Misztal, Fruzsina R. Walter, Maria A. Deli, Hiroshi Ishikawa, Horst Schroten, Christian Schwerk, and et al. 2021. "Effect of Lipopolysaccharide-Induced Inflammatory Challenge on β-Glucuronidase Activity and the Concentration of Quercetin and Its Metabolites in the Choroid Plexus, Blood Plasma and Cerebrospinal Fluid" International Journal of Molecular Sciences 22, no. 13: 7122. https://doi.org/10.3390/ijms22137122