Ectopic Expression of Grapevine Gene VaRGA1 in Arabidopsis Improves Resistance to Downy Mildew and Pseudomonas syringae pv. tomato DC3000 But Increases Susceptibility to Botrytis cinerea

The protein family with nucleotide binding sites and leucine-rich repeat (NBS-LRR) in plants stimulates immune responses caused by effectors and can mediate resistance to hemi-biotrophs and biotrophs. In our previous study, a Toll-interleukin-1(TIR)-NBS-LRR gene cloned from Vitis amurensis “Shuanghong”, VaRGA1, was induced by Plasmopara viticola and could improve the resistance of tobacco to Phytophthora capsici. In this study, VaRGA1 in “Shuanghong” was also induced by salicylic acid (SA), but inhibited by jasmonic acid (JA). To investigate whether VaRGA1 confers broad-spectrum resistance to pathogens, we transferred this gene into Arabidopsis and then treated with Hyaloperonospora arabidopsidis (Hpa), Botrytis cinerea (B. cinerea), and Pseudomonas syringae pv. tomato DC3000 (PstDC3000). Results showed that VaRGA1 improved transgenic Arabidopsis thaliana resistance to the biotrophic Hpa and hemi-biotrophic PstDC3000, but decreased resistance to the necrotrophic B. cinerea. Additionally, qPCR assays showed that VaRGA1 plays an important role in disease resistance by activating SA and inhibiting JA signaling pathways. A 1104 bp promoter fragment of VaRGA1 was cloned and analyzed to further elucidate the mechanism of induction of the gene at the transcriptional level. These results preliminarily confirmed the disease resistance function and signal regulation pathway of VaRGA1, and contributed to the identification of R-genes with broad-spectrum resistance function.


Introduction
Grape is an economically important crop cultivated worldwide. However, its yield and quality are restricted by various pathogens [1,2]. Repeated use of fungicides not only increases production costs and causes environmental pollution, but also leads to the drug resistance of pathogens, which may eventually cause sudden pathogen outbreaks in the future [3,4]. As an alternative to chemical control, planting resistant varieties is cost-effective and environmentally friendly [5]. Utilizing disease-resistant resources of wild grape has become one of the most economical and effective methods to improve the resistance of cultivated varieties [6,7].
Plants generally have two immune system levels. First, transmembrane pattern recognition receptors are used to identify slowly evolving pathogen pattern molecules such as flagellin. Second, proteins of resistance (R)-gene encoding conserved nucleotide binding sites (NBS) and leucine-rich repeats (LRR) can identify pathogens in cells [8,9]. 'Gene-to-gene' resistance resulting from the direct

VaRGA1 Expression Is Affected by SA and JA in Grape
We have previously confirmed that VaRGA1 was induced by P. viticola in V. amurensis "Shuanghong" [35]. In order to explore whether the expression of VaRGA1 was affected by exogenous hormones, VaRGA1 expression levels were detected at different time points after spraying SA and JA. Results showed that VaRGA1 expression was strongly induced by SA at 1, 3, and 6 hours post inoculation (hpi), and it slowly reached its peak 12 h after treatment, then decreased rapidly at 24 and 48 hpi. The VaRGA1 expression level was about 12 times higher than the control mock inoculation. In contrast, VaRGA1 expression was suppressed by JA, decreasing to its lowest point 6 h after treatment before recovering. The expression level was less than half the mock at its lowest point, but after 48 hpi it was still lower than that of the mock (Figure 1).

VaRGA1 Expression Is Affected by SA and JA in Grape
We have previously confirmed that VaRGA1 was induced by P. viticola in V. amurensis "Shuanghong" [35]. In order to explore whether the expression of VaRGA1 was affected by exogenous hormones, VaRGA1 expression levels were detected at different time points after spraying SA and JA. Results showed that VaRGA1 expression was strongly induced by SA at 1, 3, and 6 hours post inoculation (hpi), and it slowly reached its peak 12 h after treatment, then decreased rapidly at 24 and 48 hpi. The VaRGA1 expression level was about 12 times higher than the control mock inoculation. In contrast, VaRGA1 expression was suppressed by JA, decreasing to its lowest point 6 h after treatment before recovering. The expression level was less than half the mock at its lowest point, but after 48 hpi it was still lower than that of the mock (Figure 1).

Figure 1.
Expression levels of VaRGA1 in V. amurensis "Shuanghong" following salicylic acid (SA) and methyl jasmonate (MeJA) treatments. The expression level of VaRGA1 in "Shuanghong" under nonstressed conditions was defined as 1.0. Data represent mean values ± SD from three independent experiments. Asterisks show statistically significant difference (** p < 0.01, Student's t test).

The Response of VaRGA1 in Transgenic Arabidopsis Lines to Different Pathogens
In order to study the role of VaRGA1 in the process of disease resistance, VaRGA1 expression patterns in transgenic plants inoculated with Hpa, PstDC3000, and B. cinerea were determined by quantitative real-time PCR (qRT-PCR). Results showed that gene expression was induced by Hpa ( Figure 2A) and PstDC3000 ( Figure 2B), reached a peak 48 h post-inoculation (hpi), and then decreased gradually. Overall, the expression of VaRGA1 was affected more by PstDC3000, and the expression level of VaRGA1 was higher than that of the Hpa; furthermore, the amount of expression was about thrice that of the Hpa at 24 hpi. However, the gene expression level was inhibited by B. cinerea ( Figure 2C), the expression level decreased gradually after infection, reached the lowest level of 48 hpi, and then increased gradually. At 96 hpi, the expression levels of this gene in L2 and L3 were both significantly lower than that before infection, and the difference was not significant for L1. These results suggest that VaRGA1 may play different roles in the process of plant resistance to different pathogens. Expression levels of VaRGA1 in V. amurensis "Shuanghong" following salicylic acid (SA) and methyl jasmonate (MeJA) treatments. The expression level of VaRGA1 in "Shuanghong" under non-stressed conditions was defined as 1.0. Data represent mean values ± SD from three independent experiments. Asterisks show statistically significant difference (** p < 0.01, Student's t test).

The Response of VaRGA1 in Transgenic Arabidopsis Lines to Different Pathogens
In order to study the role of VaRGA1 in the process of disease resistance, VaRGA1 expression patterns in transgenic plants inoculated with Hpa, PstDC3000, and B. cinerea were determined by quantitative real-time PCR (qRT-PCR). Results showed that gene expression was induced by Hpa ( Figure 2A) and PstDC3000 ( Figure 2B), reached a peak 48 h post-inoculation (hpi), and then decreased gradually. Overall, the expression of VaRGA1 was affected more by PstDC3000, and the expression level of VaRGA1 was higher than that of the Hpa; furthermore, the amount of expression was about thrice that of the Hpa at 24 hpi. However, the gene expression level was inhibited by B. cinerea ( Figure 2C), the expression level decreased gradually after infection, reached the lowest level of 48 hpi, and then increased gradually. At 96 hpi, the expression levels of this gene in L2 and L3 were both significantly lower than that before infection, and the difference was not significant for L1. These results suggest that VaRGA1 may play different roles in the process of plant resistance to different pathogens.

Expression of VaRGA1 in Arabidopsis Improves Resistance to Hpa
To investigate the role of VaRGA1 in the process of resistance to Hpa, we counted the phenotype, spore number per gram, and histochemical staining of different lines. The transgenic plants showed fewer signs of necrotic leaves and spore growth seven days post-inoculation (dpi) ( Figure 3A) and had lower spore numbers per gram of 5 dpi ( Figure 3B) than the control (Col-0). The mutant plants showed more signs of necrotic leaves and spore growth at 7 dpi, and had significantly higher spore numbers per gram at 5 dpi than the control. Diaminobenzidine (DAB), trypan blue, and Nitro blue tetrazolium (NBT) staining were effective methods to evaluate the degree of disease resistance of the plants, and were used to detect H2O2 accumulation, cell death, and superoxide anion (O2 -) accumulation, respectively. The depth of staining was positively correlated with the disease resistance of plants. Staining results showed that transgenic plants had more H2O2 and O2accumulation than the control, while the mutants had less H2O2 and O2 -accumulation than the control ( Figure 3C). Mock treatments of histochemical staining following inoculation with Hpa are shown in Supplementary Figure S3A. These results suggest that expression of VaRGA1 can enhance the resistance of A. thaliana to Hpa.

Expression of VaRGA1 in Arabidopsis Improves Resistance to Hpa
To investigate the role of VaRGA1 in the process of resistance to Hpa, we counted the phenotype, spore number per gram, and histochemical staining of different lines. The transgenic plants showed fewer signs of necrotic leaves and spore growth seven days post-inoculation (dpi) ( Figure 3A) and had lower spore numbers per gram of 5 dpi ( Figure 3B) than the control (Col-0). The mutant plants showed more signs of necrotic leaves and spore growth at 7 dpi, and had significantly higher spore numbers per gram at 5 dpi than the control. Diaminobenzidine (DAB), trypan blue, and Nitro blue tetrazolium (NBT) staining were effective methods to evaluate the degree of disease resistance of the plants, and were used to detect H 2 O 2 accumulation, cell death, and superoxide anion (O 2 − ) accumulation, respectively. The depth of staining was positively correlated with the disease resistance of plants.
Staining results showed that transgenic plants had more H 2 O 2 and O 2 − accumulation than the control, while the mutants had less H 2 O 2 and O 2 accumulation than the control ( Figure 3C). Mock treatments of histochemical staining following inoculation with Hpa are shown in Supplementary Figure S3A. These results suggest that expression of VaRGA1 can enhance the resistance of A. thaliana to Hpa.

Assessment of Defense-Related Gene Expression After Inoculating Hpa
SA-dependent defenses play an active role in plant resistance to Hpa, an Arabidopsis biotroph. The expression of AtNPR1 ( Figure 4A) and AtEDS1 ( Figure 4B), the key genes of SA signaling pathway in different lines, was measured at different time points after inoculation. The relative expression level of both genes was significantly higher in transgenic lines than in the control (Col-0) before inoculation, but was significantly lower in varga1. The expression of AtNPR1 ( Figure 4C) and AtEDS1 ( Figure 4D) in the transgenic lines reached peaks at 48 and 24 hpi, respectively, and then decreased gradually. The expression of the two genes decreased significantly at 96 hpi, close to the level before the inoculation. Col-0 and varga1 also showed similar trends, but had significantly lower expression levels than transgenic lines. Therefore, the order of expression of both genes was L1 > L2 >L3 > Col-0 > varga1 (mutants). JA-dependent defense is another signaling pathway involved in plant disease resistance. Results showed that the key genes in this pathway, AtPR3 and LOX3, were slightly induced by Hpa, their expression reaching a peak at 48 hpi, but the degree of induction of transgenic lines was significantly lower than that of the control (Col-0), while that of the mutants was significantly higher than that of the control. The expression of AtNPR1 and AtEDS1 of all lines reached the highest level at 48 hpi and decreased close to the level before the inoculation at 48 hpi.

Assessment of Defense-Related Gene Expression after Inoculating Hpa
SA-dependent defenses play an active role in plant resistance to Hpa, an Arabidopsis biotroph. The expression of AtNPR1 ( Figure 4A) and AtEDS1 ( Figure 4B), the key genes of SA signaling pathway in different lines, was measured at different time points after inoculation. The relative expression level of both genes was significantly higher in transgenic lines than in the control (Col-0) >L3 > Col-0 > varga1 (mutants). JA-dependent defense is another signaling pathway involved in plant disease resistance. Results showed that the key genes in this pathway, AtPR3 and LOX3, were slightly induced by Hpa, their expression reaching a peak at 48 hpi, but the degree of induction of transgenic lines was significantly lower than that of the control (Col-0), while that of the mutants was significantly higher than that of the control. The expression of AtNPR1 and AtEDS1 of all lines reached the highest level at 48 hpi and decreased close to the level before the inoculation at 48 hpi.

Expression of VaRGA1 in Arabidopsis Improves Resistance to PstDC3000
To explore whether VaRGA1 plays an important role in the anti-PstDC3000 mechanism of A. thaliana, the leaf phenotype, spore number, and histochemical staining of 5-week-old seedlings after inoculation were assessed. Only a small amount of mottled yellowing was observed in the leaves of transgenic lines, compared with the large area of yellowing in the leaves of Col-0 and the yellowing and even wilting in the leaves of atrga1 at 5 dpi ( Figure 5A). On the second day after inoculation, the spore number of the transgenic lines was significantly lower than that of Col-0 and atrga1 ( Figure 5B). The results of trypan blue staining at 3 dpi showed that the leaves of the transgenic lines were darker blue when compared to those of Col-0 and atrga1, indicating that the local leaf necrosis area of the transgenic lines in the early stage of infection was larger than that of Col-0 and atrga1. The results of DAB staining showed that the yellowing area of the leaves was larger and the accumulation of reactive oxygen species (ROS) was higher in the transgenic lines than in Col-0 and atrga1. Aniline blue staining showed that the transgenic lines had more callose accumulation than did Col-0 and atrga1 ( Figure 5C). Mock treatments of histochemical staining following inoculation with PstDC3000 were shown in Supplementary Figure S3B. These results showed that the transgenic lines were more

Expression of VaRGA1 in Arabidopsis Improves Resistance to PstDC3000
To explore whether VaRGA1 plays an important role in the anti-PstDC3000 mechanism of A. thaliana, the leaf phenotype, spore number, and histochemical staining of 5-week-old seedlings after inoculation were assessed. Only a small amount of mottled yellowing was observed in the leaves of transgenic lines, compared with the large area of yellowing in the leaves of Col-0 and the yellowing and even wilting in the leaves of atrga1 at 5 dpi ( Figure 5A). On the second day after inoculation, the spore number of the transgenic lines was significantly lower than that of Col-0 and atrga1 ( Figure 5B). The results of trypan blue staining at 3 dpi showed that the leaves of the transgenic lines were darker blue when compared to those of Col-0 and atrga1, indicating that the local leaf necrosis area of the transgenic lines in the early stage of infection was larger than that of Col-0 and atrga1. The results of DAB staining showed that the yellowing area of the leaves was larger and the accumulation of reactive oxygen species (ROS) was higher in the transgenic lines than in Col-0 and atrga1. Aniline blue staining showed that the transgenic lines had more callose accumulation than did Col-0 and atrga1 ( Figure 5C). Mock treatments of histochemical staining following inoculation with PstDC3000 were shown in Supplementary Figure S3B. These results showed that the transgenic lines were more resistant to PstDC3000 than Col-0 and atrga1, with the following order of disease resistance: L1 > L2 >L3> Col-0 > atrga1. resistant to PstDC3000 than Col-0 and atrga1, with the following order of disease resistance: L1 > L2 >L3> Col-0 > atrga1.

Assessment of the Expression of Defense-Related Genes after Inoculating PstDC3000
The expression levels of key genes AtNPR1 ( Figure 6A) and AtEDS1 ( Figure 6B) in the SA signaling pathway were detected at different time points after the inoculation of PstDC3000 to explore the signaling pathway involved in the resistance of transgenic A. thaliana. The expression of AtNPR1 and AtEDS1 in the transgenic lines increased rapidly after infection, and was significantly higher than that in the control (Col-0) and mutant (atrga1), reached a peak at 48 and 24 hpi, respectively, and then decreased gradually. The expression of AtNPR1 decreased slowly, and its expression level was 5-10 times higher than that before infection at 96 hpi. The expression of AtEDS1 was at a lower level at 72 hpi and was close to the level before the inoculation at 96 hpi. Although the expression of the key genes AtPR3 ( Figure 6C) and LOX3 ( Figure 6D) in the JA signaling pathway of all lines increased after inoculation, the expression level in the transgenic lines was significantly lower than that in Col-0, especially at 48 and 72 hpi, but the expression level was significantly higher in atrga1 than in Col-0. These results showed that the SA and JA signaling pathways both acted in the anti-PstDC3000 response of A. thaliana, but the intensity of the former was greater.
was carried out 2 dpi. Data represent mean values ± SD from three independent experiments. Asterisks show statistically significant difference (** p < 0.01, Student's t test). (C) Histochemical staining was carried out for the detection of cell death, H2O2 accumulation, and superoxide anions (O2 -) accumulation 3 dpi for (a) trypan blue, (b) diaminobenzidine (DAB), and (c) nitro blue tetrazolium (NBT) staining, respectively. Three independent experiments were conducted with 10 leaves per experiment. The black scale bars = 10 mm. The white scale bars = 0.5 mm.

Assessment of the Expression of Defense-Related Genes after Inoculating PstDC3000
The expression levels of key genes AtNPR1 ( Figure 6A) and AtEDS1 ( Figure 6B) in the SA signaling pathway were detected at different time points after the inoculation of PstDC3000 to explore the signaling pathway involved in the resistance of transgenic A. thaliana. The expression of AtNPR1 and AtEDS1 in the transgenic lines increased rapidly after infection, and was significantly higher than that in the control (Col-0) and mutant (atrga1), reached a peak at 48 and 24 hpi, respectively, and then decreased gradually. The expression of AtNPR1 decreased slowly, and its expression level was 5-10 times higher than that before infection at 96 hpi. The expression of AtEDS1 was at a lower level at 72 hpi and was close to the level before the inoculation at 96 hpi. Although the expression of the key genes AtPR3 ( Figure 6C) and LOX3 ( Figure 6D) in the JA signaling pathway of all lines increased after inoculation, the expression level in the transgenic lines was significantly lower than that in Col-0, especially at 48 and 72 hpi, but the expression level was significantly higher in atrga1 than in Col-0. These results showed that the SA and JA signaling pathways both acted in the anti-PstDC3000 response of A. thaliana, but the intensity of the former was greater.

Expression of VaRGA1 in Arabidopsis Decreases Resistance to B. cinerea
The resistance of transgenic lines of VaRGA1 to B. cinerea was judged by assessing the area of leaf necrosis ( Figure 7A) and diameter of lesions ( Figure 7B) at 3 dpi. Not only was the disease spot diameter of the transgenic lines significantly larger than that of Col-0, but the degree of leaf infection was also more obvious, resulting in the attachment of large areas of B. cinerea. However, mutants were not as sensitive as Col-0. B. cinerea is a necrotrophic pathogen, and the accumulation of dead cells and ROS promote the infection. Histochemical staining ( Figure 7C The resistance of transgenic lines of VaRGA1 to B. cinerea was judged by assessing the area of leaf necrosis ( Figure 7A) and diameter of lesions ( Figure 7B) at 3 dpi. Not only was the disease spot diameter of the transgenic lines significantly larger than that of Col-0, but the degree of leaf infection was also more obvious, resulting in the attachment of large areas of B. cinerea. However, mutants were not as sensitive as Col-0. B. cinerea is a necrotrophic pathogen, and the accumulation of dead cells and ROS promote the infection. Histochemical staining ( Figure 7C) showed that cell death and the accumulation of H2O2 and O2 -in transgenic lines was significantly higher than in Col-0. Mock treatments of histochemical staining following inoculation with B. cinerea are shown in Supplementary Figure S3C. All experimental results showed that overexpression of VaRGA1 could decrease the resistance of A. thaliana to B. cinerea.

Assessment of the Expression of Defense-Related Genes After Inoculating B. cinerea
To fight against B. cinerea, the JA signaling pathway of plants is activated to initiate a series of downstream defense reactions. The role of VaRGA1 in the anti-B. cinerea response of A. thaliana was judged by detecting the expression of the key genes of the SA and JA signaling pathways after inoculation. The expression levels of AtNPR1 ( Figure 8A), a key gene in the SA signaling pathway, was reduced to its lowest level at 48 hpi, followed by a rapid increase at 72 hpi and a decrease at 96 hpi. The expression of the key genes AtEDS1 ( Figure 8B) in the SA signaling pathway of all lines peaked at 24 hpi and then gradually decreased. The expression of AtNPR1 and AtEDS1 was significantly higher in the transgenic lines than in Col-0 and atrga1 during the whole process. The expression levels of the two key genes, AtPR3 ( Figure 8C) and LOX3 ( Figure 8D), in the JA signaling pathway significantly increased after inoculation in the transgenic lines, but remained significantly lower in Col-0 and atrga1. The expression level of AtPR3 and LOX3 continued to increase in non-transgenic lines and reached maximums at 96 hpi. For the transgenic lines, the expression levels of AtPR3 and AtLOX3 in different lines were different, but on the whole, expression levels of AtPR3 and AtLOX3 had increased slowly and reached the maximum at 72 hpi, and then a certain degree of decrease was observed at 96 hpi.

Assessment of the Expression of Defense-Related Genes after Inoculating B. cinerea
To fight against B. cinerea, the JA signaling pathway of plants is activated to initiate a series of downstream defense reactions. The role of VaRGA1 in the anti-B. cinerea response of A. thaliana was judged by detecting the expression of the key genes of the SA and JA signaling pathways after inoculation. The expression levels of AtNPR1 ( Figure 8A), a key gene in the SA signaling pathway, was reduced to its lowest level at 48 hpi, followed by a rapid increase at 72 hpi and a decrease at 96 hpi. The expression of the key genes AtEDS1 ( Figure 8B) in the SA signaling pathway of all lines peaked at 24 hpi and then gradually decreased. The expression of AtNPR1 and AtEDS1 was significantly higher in the transgenic lines than in Col-0 and atrga1 during the whole process. The expression levels of the two key genes, AtPR3 ( Figure 8C) and LOX3 (Figure 8D), in the JA signaling pathway significantly increased after inoculation in the transgenic lines, but remained significantly lower in Col-0 and atrga1. The expression level of AtPR3 and LOX3 continued to increase in nontransgenic lines and reached maximums at 96 hpi. For the transgenic lines, the expression levels of AtPR3 and AtLOX3 in different lines were different, but on the whole, expression levels of AtPR3 and AtLOX3 had increased slowly and reached the maximum at 72 hpi, and then a certain degree of decrease was observed at 96 hpi.

Discussion
The results of our study showed that VaRGA1 expression in "Shuanghong" induced the SA pathway, inhibited the JA pathway, and additionally, VaRGA1 expression in the transgenic A. thaliana was induced by Hpa and PstDC3000 and inhibited by B. cinerea. These results suggest that VaRGA1 can enhance disease resistance and, in particular, resistance to biotrophic pathogens through the SA signaling pathway. The largest R-protein type, the NBS-LRR protein family in plants, has been recognized to stimulate immune responses caused by effectors [8,36,37]. The NBS-LRR-type R-genes are expressed at low levels before the pathogen attacks, but once the pathogen invades plant tissues or hormone levels change, they can be rapidly induced to an appropriate level to play a role in disease resistance [38][39][40]. For example, the low expression level of one coiled-coil (CC)-NBD-LRR-like R-gene cloned from riparian grape (V. riparia) under normal growth was significantly improved after inoculation with P. viticola [41]. The expression of Arabidopsis RPP8 was rapidly increased following infection by Hyaloperonospora arabidopsidis or spraying with SA hormone to respond to such biological or non-biological stress factors [42]. Our previous study showed that the expression of VaRGA1 in V. amurensis "Shuanghong" was first rapidly increased and then decreased slowly after inoculation with Hpa [35].
The regulation of transcription level is the most important mode of gene expression, but the regulation of post-transcriptional level also plays an important role in the process of gene expression. Some nuclear proteins such as STA1 are crucial for pre-mRNA splicing and the turnover of unstable transcripts in plant responses to different stresses [43]. Whether eukaryotes can use mature mRNA molecules to translate proteins for growth and development for a long time is closely related to the stability of mRNA and the release of shielding state. Under biotic and abiotic stresses, specific smRNAs were induced to regulate the mRNA stability by intersecting with most of the pathways [44]. The stability of SOS1 mRNA was greatly improved under the treatment of H 2 O 2 , salt and other ionic and dehydration [45]. The level of mRNA in VaRGA1 in this study was affected by different pathogenic bacteria, possibly because of the change in mRNA stability under stress conditions. Since many of the mRNA in the eukaryotes are extremely stable, the transcription of the strong promoter and the regulation of the specific smRNAs may also increase the level of mRNA of this gene under certain stress conditions.
B. cinerea, a common necrotrophic pathogen, can cause diseases in many plants by secreting virulence factors and cell wall degradation enzymes [40,46]. Few R-genes with resistance to B. cinerea have been reported because effector-triggered immunity induced by R-gene can activate the plant HR reaction in the early stage of pathogen infection, which is helpful to the infection and growth of necrotrophic pathogens [47,48]. This study further supported the above conclusions, showing that transgenic A. thaliana overexpressing VaRGA1 had lower resistance to B. cinerea, larger lesion area, and more spores than the other lines. However, the atrga1 mutant could improve the resistance of A. thaliana to B. cinerea. B. cinerea is tolerant to oxidative burst, and can produce ROS to promote its infection. ROS, signaling molecules that promote cell death, are an indicator of successful infection by B. cinerea [49]. Histochemical staining showed that the transgenic lines had more cell death and reactive oxygen accumulation than other lines, which helped the spread of B. cinerea. Moreover, the R-gene RPW8, which regulates resistance to powdery mildew in A. thaliana, can decrease the resistance of A. thaliana to B. cinerea [50], which is consistent with our results.
NBS-LRR genes can recognize the effectors secreted by biotrophic pathogens to mediate resistance [36]. They usually cause hypersensitivity in the place of infection, followed by ROS production, accumulation of plant hormones related to disease resistance, expression of defense-related genes, and phytophane synthesis [51,52]. The production of ROS is a necessary condition for cell death, which can restrict biotrophic pathogens from obtaining nutrients from plants [53,54]. Therefore, continuous expression of CC-NBS-LRR gene ADR1 in A. thaliana enhanced the resistance to biotrophic pathogens [55]. In our study, overexpression of VaRGA1 could enhance the resistance of A. thaliana to Hpa and PstDC3000, and significantly reduce the number of spores of these two bacteria. The results of histochemical staining showed that transgenic lines had more cell death and ROS accumulation than other lines, which helped limit the growth of biotrophic pathogens. The mutant atrga1 was more susceptible than Col-0 to Hpa and PstDC3000, which further proved the resistance of VaRGA1 to these two bacteria. However, as previously proposed, the degree of cell death and accumulation of ROS in VaRGA1 transgenic lines were significantly higher than those in Col-0, which led to the decrease of resistance to B. cinerea.
Mock is one of the most basic principles of experimental design, which is essential for obtaining reliable experimental results. The leaves of the mock in the Hpa inoculation experiment were close to transparency after staining. However, as the leaves were too small, they needed to be observed under the microscope. It was difficult to take the actual color because of the influence of microscope light. The color of stained mock leaves was uniform and lighter under the microscope, while that treated with Hpa was darker, with mottled spots. Therefore, mock treatment was effective and could reduce the influence of various uncertain factors in the experiment. The leaves treated with PstDC3000 and B. cinerea were larger and could be photographed in normal light after staining, so the mock could reduce the experimental error more intuitively. For the mock in the B. cinerea inoculation experiment, clear callose was observed by NBT staining, which might be due to the fact that the leaves to be treated need to be placed in vitro, causing their own defense and response. Even so, the callose content of the mock was significantly lower than that with other treatments, so the experimental results were reliable.
As a result of the lack of mobile defense cells and acquired immune systems, plants can only rely on the innate immunity of each cell and the systemic signals sent from the place of infection to resist pathogens [56][57][58]. The accumulation of plant hormones and signal transduction related to disease resistance are important links in the mechanism of plant disease resistance. At present, the known plant disease resistance signaling pathways mainly include the SA and JA signaling pathways, and different signaling pathways intersect into a complex signal network [59]. These two signaling pathways are usually suppressed by each other, but there have also been reports of synergy [60,61]. In this study, the expression of AtNPR1 and AtEDS1 (the key genes of the SA signaling pathway) significantly increased after inoculation with Hpa, PstDC3000, and B. cinerea. The expression of AtPR3 and AtLOX3, the key genes of the JA pathway, increased slightly, but was significantly lower than that of Col-0. In other words, the JA signaling pathway was suppressed to a certain extent. It can be inferred that VaRGA1 can activate the SA signaling pathway during pathogen infection, which can increase the resistance of A. thaliana to Hpa and PstDC3000, but decrease the resistance to B. cinerea. Previous studies have also shown that SA and its signaling pathway genes are induced by some NBS-LRR genes, which are an important component of the plant disease resistance system [62,63].
A promoter is an important element in the regulation of the transcription process, which largely determines the expression level of downstream genes [64,65]. HSC70 is a highly conserved molecular chaperone, and its promoter activity increased at high temperature, which explains the rapid increase of the expression of the HSC70 gene at high temperature [66]. The STS promoter in V. pseudoreticulata could respond to various biological and non-biological stress [64]. In our study, a 1104 bp promoter fragment of VaRGA1 was cloned and analyzed. In addition to common elements such as CAAT-box and TATA-box, some hormone-induced elements and stress elements were also predicted (Supplementary Figure S4). TCA-element was involved in SA responsiveness, and MBS was involved in drought-inducibility. These elements are likely to promote the expression of VaRGA1 under certain conditions. Currently, only a few R-genes with broad-spectrum resistance function have been reported, which greatly limits the development of molecular breeding. Fortunately, some studies have found that overexpression of NBS-LRR genes such as NLS1 and WRR4 can activate the SA signaling pathway and increase the expression of defense-related genes allowing broad-spectrum resistance [67,68]. Similarly, we have demonstrated that VaRGA1 could improve the resistance of transgenic A. thaliana to Hpa and PstDC3000 by activating the SA signaling pathway, but decrease the resistance to B. cinerea. In summary, our study preliminarily confirmed the disease resistance function of VaRGA1 and its signal regulation pathway, and made some contributions to the identification of R-genes with broad-spectrum resistance function. However, its detailed mechanism needs to be further studied. Future research will focus on finding the core region of the upstream promoter of VaRGA1 and the protein that interacts with the gene.

Plant Materials and Pathogens
The annual V. amurensis "Shuanghong", wildtype A. thaliana (Col-0), and pad4 mutant were kindly provided by our laboratory. The mutants of A. thaliana gene AT5G36930. (atrga1), which are homologous to VaRGA1, were purchased from NASC [69]. V. amurensis "Shuanghong" was planted in a flowerpot containing a 1:1 mixture of nutritious soil and vermiculite, then grown in a greenhouse at a temperature of 25 • C and a 16-h light/8-h dark cycle. The plants were watered every week and fertilizer was applied every other month. Arabidopsis were grown in a chamber at 25 • C and a 16-h light/8-h dark photoperiod. Hpa (At) was cultured on 10-day-old pad4 mutants. PstDC3000 was maintained on lysogeny broth (LB) medium containing 50 mg·L −1 rifampicin at 30 • C. B. cinerea was maintained on a potato dextrose agar (PDA) medium containing 100 mg·L −1 kanamycin at 23 • C in the dark.

Hormone Treatments in Grape
Solutions of 100 mM SA (Tokyo, Japan) and 50 mM methyl jasmonate (MeJA, Haarlem, The Netherlands) were sprayed on grape leaves with the same growth conditions, and aseptic ultra-pure water was sprayed as the mock [70]. The expression level of VaRGA1 was determined at 0, 1, 3, 6, 12, 24, and 48 h post-treatment.

Generation of Transgenic Plants
The coding sequence of VaRGA1 was amplified by PCR using the vector named pBI121-VaRGA1-GFP from our previous work [35]. The specific homologous recombination primers were pHB-VaRGA1-GFP-F (5 -agcttggatccagaactagtATGTCAATCGGCATGGATC-3 ) and pHB-VaRGA1-GFP-R (5 -cccttgctcaccatactagtTGCAAAAGAGAGCAAAGTTC-3 ). The lowercase letters in the above primers represent the nucleotides with restriction sites, and the uppercase letters represent the nucleotides complementary to the VaRGA1 gene. The PCR product was then cloned downstream of the cauliflower mosaic virus (CaMV) 35S promoter of pHB-35S:EGFP, a plant over-expression vector. Subsequently, the recombinant plasmid named pHB-VaRGA1-GFP was introduced into Agrobacterium (strain GV3101) that were used to transform A. thaliana (Col-0), according to an existing protocol [71]. Seventy transgenic lines were obtained, and three lines (L1, L2, and L3) with the highest expression induced by Hpa were chosen for selecting T4 generation homozygous plants for subsequent experiments. RT-PCR detection and subcellular localization of the transgenic lines are shown in Supplementary Figures S1 and S2.

Inoculation of Pathogens
The downy mildew (causal agent Hpa) treatment was conducted following a previously described method [42]. The seeds of transgenic, mutant, and wild type A. thaliana were grown evenly in fertile soil at 25 seeds per pot. Arabidopsis downy mildew solution was evenly sprayed on the leaves and cultured at 90% humidity for seven days. Histochemical detection and spore number calculation were carried out at five days post inoculation (dpi). Leaf necrosis area and spore growth were observed at 7 dpi.
PstDC3000 was activated by fresh LB liquid medium (28 • C, 200 rpm) [72]. The optical density at 600 nm (OD600) of the secondary activation was 0.6. After bacterial cell collection (5000 rpm, 5 min), cells were suspended in aseptic 10 mm MgCl 2 solution (0.05% Silwet-77) and OD600 adjusted to 0.004. Four-week-old A. thaliana plants were selected and the backs of leaves were injected with PstDC3000 using a 1-mL aseptic syringe. After inoculating all leaves, the plants were placed in a black tray, covered with white transparent plastic, and sprayed with water to moisturize properly [72]. Spore number statistics were assessed at 2 dpi, and histochemical detection was carried out 3 dpi. Area of necrosis and yellowing of the leaves were assessed at 5 dpi.
The B. cinerea inoculation method followed a previously described protocol [73]. First, B. cinerea was cultured on PDA culture medium at 23 • C. After about 21 days, the mycelium and the spore were suspended in the inoculation liquid of 4% maltose and 1% peptone. The residual body of the hypha was removed by filtration. Inoculant of the mycotic spore suspension was prepared by counting and adjusting the concentration of the mycotic spores to 2−106 mL −1 with a blood cell counting plate. Four-week-old leaves of A. thaliana were cut to inoculate 10 µL spore suspension on the near-axis surface. Water mist was sprayed in the tray and was moisture-maintained by a preservative film. Leaves were cultured at 23 • C. Histochemical detection, spore number statistics, and the disease state of the leaf blade were conducted 3 dpi.
To detect H 2 O 2 accumulation, plant materials were put in 1 mg·mL −1 DAB for overnight dyeing in the dark. Then, 70% ethanol was used for decolorizing. Cell death was assessed by trypan blue staining [74]. The materials were placed in boiled trypan blue solution (a 1:1:1:1:1 ratio of ddH 2 O, trypan blue, phenol, glycerol, and lactic acid) for 5 min and decolorized in 2.5 g·mL −1 chloral hydrate for 1-2 days. Superoxide anion (O 2 − ) accumulation can be detected by NBT staining [75]. Additionally, 6 mM NBT was dissolved in Hepes buffer solution (pH 7.5) and the leaves of Arabidopsis were dyed in NBT dyeing solution for 2 h. Then, leaves were decolorized in a 95% ethanol water bath at 50 • C for 1 hour.

Expression Analysis of Related Genes by Quantitative Real-Time PCR
The total RNA of plant materials was extracted using a Plant RNA Kit (R6827-02; Omega Bio-tek, GA, USA). RNA purity and concentration were detected by 1% agarose gel electrophoresis and nanodrop2000, respectively. Reverse transcription of the extracted RNA was completed with TransScript (AT311-03; TaKaRa Biotechnology, Dalian, China), and the obtained cDNA was diluted six times. Quantitative real-time PCR (qRT-PCR) was conducted using a previously described method [35]. The primers used for qRT-PCR are listed in Supplementary Table S1. Leaf samples were collected at different time points for subsequent gene expression analyses. For the detection of VaRGA1 expression induced by SA and JA in grape, leaf samples were collected at 0, 1, 3, 6, 12, 24, and 48 hpi. To detect the expression of VaRGA1 and defense-related genes in transgenic Arabidopsis lines after inoculating different pathogens, leaf samples were collected at 0, 24, 48, 72, and 96 hpi.

Plant DNA Extraction and VaRGA1 Promoter Cloning
Extraction of the genomic DNA of V. amurensis "Shuanghong" was conducted using the hexadecyl trimethyl ammonium Bromide (CTAB) method. Primers (PVa-F and PVa-R; listed in Supplementary Table S1) were designed according to the previously cloned VaRGA1 to obtain 1242 bp PCR products including specific upstream sequences and coding sequences (CDS). PCR amplification and vector construction were conducted following a previously described method [76]. The obtained sequences were analyzed by PlantCARE [77].

Statistical Analysis
Three biological replicates and three technical replicates were conducted for each experiment. Data analysis and plotting were conducted in Microsoft Excel (Microsoft Corporation, Redmond, WA, USA) and SigmaPlot 14.0 (Systat, Inc., Point Richmond, CA, USA). Significant differences were detected by SPSS Statistics 17.0 (IBM China Company Ltd, Beijing, China). (Student's t test, p < 0.01).