Reduction of Pericarp Browning and Microbial Spoilage on Litchi Fruits in Modiﬁed Atmosphere Packaging

: The pericarp browning and postharvest microbiological decay of litchi fruit ( Litchi chinensis Sonn cv Kwai Mai) signiﬁcantly reduce its commercial potential in the fresh market. In this study, different combinations of modiﬁed atmosphere packaging (MAP) were applied at 5 ± 1 ◦ C based on the use of natural gases that are innocuous to human health and an alternative to commercially adopted sulfur dioxide (SO 2 ) treatment. The results showed that control fruits, after 6 days of storage, begin to show the ﬁrst symptoms of decay, revealed by the appearance of lesions and microbial infections determined by total mesophilic microorganisms and molds. This is not the case in the MAP-treated fruits and the MAP 3-treated (5% O 2 + 20% CO 2 + 75% N 2 ) fruits that show the best results. The control fruits, moreover, turned completely brown by the end of the storage period. The MAP 3 treatment was the most effective in preventing browning and the loss of the red pericarp color and vitamin content and in maintaining acceptable SSC/TA levels and ﬂavor. In addition, a microbiological analysis revealed that all the MAP-treated litchi fruits did not harbor undesirable microorganisms during the entire cold storage period. In conclusion, the MAP 3 conditions delayed pericarp browning and maintained the better organoleptic quality of litchi fruits.


Introduction
The litchi fruit (Litchi chinensis Sonn.) belongs to tropical and subtropical regions, but today, it is grown in many regions worldwide. More recently, thanks to climate change, it has also spread to Italy where it is cultivated in Sicily [1], along with other tropical species, such as mango [2], avocado [3], papaya [4] and loquat [5], following a process that can be defined as the 'tropicalization' of Mediterranean fruit cultivation. Litchi is a nonclimacteric crop that stands out due to its bright red color, shiny epicarp and the juiciness of its flesh [6,7]. As a result of several studies, it has gained increasing commercial interest for its organoleptic and nutraceutical value [8][9][10]. However, its high perishability results in a short shelf life due to a great physiological change after harvest [11]. Peel browning and pathological decay are its main problems, as they damage its brilliance. The main causes of the browning phenomenon are due to peroxidase (POD), polyphenol oxidase (PPO) and laccase (LAC), respectively [12]. Furthermore, it was observed that membrane destruction accelerates enzymatic browning and reactive oxygen species (ROS) overproduction in fruit. Thus, the preservation of the membrane structure is useful for maintaining its quality and extending its shelf life. The key enzyme responsible for the degradation of phospholipids is Phospholipase D (PLD), which occurs as a lightning event in postharvest senescent tissues [13]. This enzyme catalyzes products, including linoleic and phosphatidic acids, that act as signals to activate the oxylipin and signal transduction pathway cellular signal, which

Vegetal Material
Four kg of litchi fruit (Litchi chinensis Sonn. cv Kwai Mai) were taken from the 'COLLURA' farm (38 • 03 30.0" N 14 • 36 40.9" E) in Sant'Agata di Militello (ME), Sicily. In total, 200 fruits were picked at commercial harvest, using skin color as an index of maturity [41,42]. Litchi fruits of regular shape and uniform size without any defects were selected. The fruit were harvested at commercial maturity from a homogenous size and checked for biotic or abiotic changes on the peel. Commercial maturity was assessed based on the ripening index (MI), which, as reported by Underhill and Wong [43], recommends a Brix • : total acid ratio of at least 40 for harvesting. The samples were previously sanified using a 2% sodium hypochlorite solution for 20 min and then allowed to dry.

Daily Pathological Surveys
Surveys were carried out for a period of 9 days and consisted of a visual evaluation of symptoms (lesions) and signs (molds) on each fruit. The contamination level was assessed according to an empiric classification suggested by Saleh and Al-Thani [45]: No

Decay Index (DI)
The decay index was evaluated according to the methodology by Morcia et al. [46], Barrera Bello et al. [47] and Perdones et al. [48]. The physical damage due to the presence of fungi and, therefore, the peel deterioration was visually evaluated and calculated through the following Equation (1): where n is the number of fruits classified for each level of contamination, and N is the total number of fruits analyzed for each treatment in the considered interval.

Peel Color
A Minolta colorimeter (Chroma Meter CR-400, Konica Minolta Sensing Inc., Tokyo, Japan) was used to evaluate the peel color change. The color space is shown as follows: brightness (L* value), redness (a*) and yellowness (b*), using the CIELAB L*a*b* system.
Prior to the analysis of the samples, the instrument was calibrated using a standard white plate. In addition, with the values of L*, a* and b*, the browning of the litchi was calculated using the formulas of Ruangchaket and Sajjaanantakul [49] Equation (2): where x = (a* + 1.75L*)/(5.645L* + a* − 0.3012b*) 2.6. Soluble Solids Content (SSC) and Titratable Acidity (TA) The juice was extracted from the litchi by means of a centrifuge (Ariete, Florence, Italy), and it was used to determine the SSC (expressed as • Brix) using a digital refractometer (Atago Co., Ltd., Tokyo, Japan). The titratable acidity (TA, expressed as g of malic acid per L) was determined using a Crison compact titrator pH meter (Crison Instruments, SA, Barcelona, Spain). Titration was carried out to a pH point of 8.2, using 5 mL of juice diluted with 50 mL of distilled water.

Firmness (F)
Fruit firmness was assessed using a texture analyzer TA.XTplus (Stable Microsystems, Ltd., Surrey, UK) with a load cell of 25 kg and test speed of 0.5 mm/s. Texture was evaluated on 5 fruit × 3 replicates.

Maturity Index (M.I.)
It was determined through the following equation described by Peralta-Ruiz et al. [50]:

Proximate Analysis
Chemical composition of the flesh was determined through the AOAC (Association of Official Analytical Chemists) methods [51] in terms of water content and total sugar content. Potassium (K), sodium (Na), calcium (Ca) and iron (Fe) were determined using atomic absorption spectroscopy following wet mineralization, while phosphorus (P) was determined using a colorimetric method [52,53].

Vitamin Analysis
Thiamine (Vit. B1), Riboflavin (Vit. B2), Niacin (Vit. B3) and Ascorbic Acid (Vit. C) were extracted and determined according to previously reported methods. Vit. B1, B2 and B3 were extracted using 0.1 N HCl [54] or a solution of 1% (v/v) H 2 SO 4 [55]. Quantification was performed via HPLC equipped with a fluorometric detector [54,55]. Finally, Vit. C was extracted with 10 mL of 1% (v/v) HPO 3 for 45 min from dried extract previously prepared. After filtration, 1 mL was mixed with 9 mL of C 12 H 7 NCl 2 O 2 , and the absorbance was measured at 515 nm against a blank after 30 min. Vitamin C was quantified using a calibration curve of authentic L-ascorbic acid (0.02-0.12 mg/100 g). All analyses were performed in triplicate, and data were expressed as mg per 100 g [53].

Organic Acid Analysis
Organic acids were extracted according to the procedure of Ding et al. [56]. One gram of frozen tissue was ground in 3 mL of 95% (v/v) ice-cold methanol, and the mixture was shaken for 10 min and filtered. The pellet was twice more extracted and with 3 mL of 80% (v/v) ice-cold methanol each time. The filtrate collected was combined and evaporated under vacuum at <35 • C until the methanol was removed. The residue was dissolved in 25 mL water; the solution was then centrifuged to remove undissolved materials. One milliliter of supernatant was passed through a 0.45 µm membrane filter, and the filtrate was then analyzed using high-performance ion-exchange chromatography (HPIC, Dionex Ultimate 3000, Dionex Corp., Sunnyvale, CA, USA) according to the method of Chen et al. [57].

Microbiological Analysis
The hygiene and safety aspects of CTR and MAP-treated litchi fruits were evaluated using plate counts. Each fruit (15 g) was firstly homogenized by a stomacher [58] and then serially diluted to a ratio of 1:10 [59]. Cell suspensions were plated on selective agar media to allow the development of total mesophilic microorganisms (TMMs) on plate count agar (PCA) ( All plate counts were performed using the spread plate method, except those for the members of Enterobacteriaceae family, which were plated using pour plate [44]. Microbiological analyses were carried out in triplicate at each sampling time.

Sensory Analysis
Ten semitrained judges with extensive experience in the sensory evaluation of food performed a hedonic liking test on litchi fruit previously prepared for sensory analysis. The method used a 9-point hedonic scale (1 = 'extremely disliked', 5 = 'neither liked nor disliked', 9 = 'extremely liked') [22,60]. The panel test consisted of a total of 15 parameters: flesh color (FC); firmness (C), fruity odor (FO), exotic fruit odor (EXO), off-odor (OFO), sweet (S), acid (A), juicy (J), astringent (AS), pungent (PU), fruit flavor (FRF), exotic fruit flavor (EXF), alcohol flavor (ALF), off-flavor (OFF) and overall evaluation (OVE). These parameters were chosen for the sensory analysis of litchi fruit at day 0 (as fresh fruit) and after 3, 6 and 9 days of cold storage and were performed both for treated and untreated samples. Furthermore, at the end of each tasting, a glass of water was provided to the consumers to rinse their mouths.

Statistical Analysis
All data collected, presented as mean ± standard deviation (SD), were subjected to statistical analysis according to an experimental factorial scheme with 5 repetitions (n = 5), using the statistical package Minitab 17.1 (Minitab Inc., State College, PA, USA, 2013). For each experimental variable, the analysis of variance (ANOVA) was performed, and significant differences among mean values were appreciated using Tukey's test at p ≤ 0.05. For each treatment, different letters indicate significant differences between sampling dates.

Daily Pathological Surveys and Decay Index (DI)
The visual observation of both the CTR and treated litchi fruits was carried out throughout the course of the experiment (d0; d3; d6; and d9) to assess the possible presence of pathogenic microorganisms on their epicarp. As shown in Figure 1, the different treatments best preserved the fruit during the experiment, acting as an inhibitory medium against pathogens. We can see an increase in the appearance of pathological lesions from day 6 of storage onwards (d6). This was more visible in the CTR fruits than in the MAP-treated fruits. The MAP 3 treatment (5% O 2 + 20% CO 2 + 75% N 2 ) provided the best results in controlling decay; this treatment showed no lesions or signs for the entire duration of storage. Currently, synthetic fungicides, chemical preservatives and fumigation with sulphur dioxide (SO 2 ) are the most popular methods of delaying fruit decay and prolonging shelf life [61]. However, the use of chemicals has generated increasing public concern over the potential risks to human health and environmental pollution. Furthermore, as reported by Sivakumar [62], the pericarp of litchi consists of numerous segments, resulting in a rough surface structure. During storage, spores germinate, penetrate the pericarp of the fruit through microfractures that form during fruit development, packaging operations or transport and subsequently, colonize the surface of the fruit [62]. According to Wang et al. [63], commercial fumigation with SO 2 also intensified microfracturing in litchi fruit. Microcracks provide a gateway for microorganisms to colonize the fruit surface [64]. The MAP treatments, therefore, can probably be inferred to have reduced the incidence of microfractures in the fruit and, furthermore, as reported by Hess-Pierce and Kader [65], the incidence of lesions may have been reduced by CO 2 exposure. Finally, Palou et al. [66] also found a clear inhibition of fungal growth on naturally infected 'Wonderful' pomegranates exposed to 5 kPa O 2 and 15 kPa CO 2 at 8.9 • C. . CTR: control fruit; MAP 1: (10% O2 + 20% CO2 + 70% N2); MAP 2: (20% O2 + 20% CO2 + 60% N2); MAP 3: (5% O2 + 20% CO2 + 75% N2). Lowercase letters indicate differences for the same treatment during the storage period. Where there are no letters, differences are not statistically significant.

Peel Color Decay and Browning Index
Chromaticity parameters L* and a* in the control and treated fruit underwent changes over the 9-day storage period ( Figure 2). As can be seen in Figure 2a,b, compared to the control fruit, slower decreases in L* and a* values were observed in the MAP-treated fruit, suggesting that brightness and redness persisted in the MAP-treated fruit. As we can see from Figure 2b, the parameter a* increased more in the CTR fruits than in the MAP-treated fruits. Moreover, the increase is particularly evident from the sixth day of storage (d6) and was confirmed by analyses of epicarp browning. The typical bright red skin color of these fruits is a commercially important parameter for consumer purchase [67], and postharvest decay and pericarp browning were identified as major problems restricting the expansion of the industry in litchi-exporting countries [11]. These problems are mainly attributed to the degradation of red pigments, oxidation of peel phenols and explosion of reactive oxygen species (ROS) [68]. According to some studies, their red color (a* value) tends to increase, moving into brown tones. Usually, the variation of red is due to the rapid degradation of flavones, carotenoids and chlorophyll [17]. Similar results were obtained in red raspberry and mulberry by studying the degradation kinetics of the anthocyanins of their juice [69,70]. On the other hand, the lowest O2 concentration in the MAP 3 package (5% O2 + 20% CO2 + 75% N2) would seem to relate back to a positive effect on the visual quality of the fruit. The presence of high N2 concentrations, on the other hand, seems to have maintained the initial characteristics of the package. In fact, the CTR bags were gas-free at the end of the storage period, while the bags with MAP 2 (20% O2 + 20% CO2 + 60% N2) were slightly more deflated than the MAP 1 (10% O2 + 20% CO2 + 70% N2) and MAP 3 (5% O2 + 20% CO2 + 75% N2) ones, which were characterized by a higher percentage of N2 in the gas mixture. Browning of the peel in the CTR fruits showed a continuous increasing trend until the end of the storage period ( Figure 3). In the MAPtreated fruit, the BI remained at values around 40% until the sixth day of storage (d6). This value then increased slightly in the treated fruit compared to the CTR fruit, which showed a BI value of 60% on the ninth day of storage.

Peel Color Decay and Browning Index
Chromaticity parameters L* and a* in the control and treated fruit underwent changes over the 9-day storage period ( Figure 2). As can be seen in Figure 2a,b, compared to the control fruit, slower decreases in L* and a* values were observed in the MAP-treated fruit, suggesting that brightness and redness persisted in the MAP-treated fruit. As we can see from Figure 2b, the parameter a* increased more in the CTR fruits than in the MAP-treated fruits. Moreover, the increase is particularly evident from the sixth day of storage (d6) and was confirmed by analyses of epicarp browning. The typical bright red skin color of these fruits is a commercially important parameter for consumer purchase [67], and postharvest decay and pericarp browning were identified as major problems restricting the expansion of the industry in litchi-exporting countries [11]. These problems are mainly attributed to the degradation of red pigments, oxidation of peel phenols and explosion of reactive oxygen species (ROS) [68]. According to some studies, their red color (a* value) tends to increase, moving into brown tones. Usually, the variation of red is due to the rapid degradation of flavones, carotenoids and chlorophyll [17]. Similar results were obtained in red raspberry and mulberry by studying the degradation kinetics of the anthocyanins of their juice [69,70]. On the other hand, the lowest O 2 concentration in the MAP 3 package (5% O 2 + 20% CO 2 + 75% N 2 ) would seem to relate back to a positive effect on the visual quality of the fruit. The presence of high N 2 concentrations, on the other hand, seems to have maintained the initial characteristics of the package. In fact, the CTR bags were gas-free at the end of the storage period, while the bags with MAP 2 (20% O 2 + 20% CO 2 + 60% N 2 ) were slightly more deflated than the MAP 1 (10% O 2 + 20% CO 2 + 70% N 2 ) and MAP 3 (5% O 2 + 20% CO 2 + 75% N 2 ) ones, which were characterized by a higher percentage of N 2 in the gas mixture. Browning of the peel in the CTR fruits showed a continuous increasing trend until the end of the storage period ( Figure 3). In the MAP-treated fruit, the BI remained at values around 40% until the sixth day of storage (d6). This value then increased slightly in the treated fruit compared to the CTR fruit, which showed a BI value of 60% on the ninth day of storage. The columns indicate the means, and the bars indicate the standard deviation (n = 5). Lowercase letters indicate significant differences (p < 0.05) between treatments on the same storage day; uppercase letters indicate significant differences (p < 0.05) for the same treatment throughout the storage period (d0 to d9). Color decay in terms of (a) L* (brightness) and (b) a* (redness) during 9 days of storage. The columns indicate the means, and the bars indicate the standard deviation (n = 5). Lowercase letters indicate significant differences (p < 0.05) between treatments on the same storage day; uppercase letters indicate significant differences (p < 0.05) for the same treatment throughout the storage period (d0 to d9).
Browning of the pericarp, as a marker of senescence, is a primary factor limiting the quality and shelf life of litchi fruits [9]. In the present study, the lowest O 2 concentration in the MAP 3 package (5% O 2 + 20% CO 2 + 75% N 2 ) would seem to relate back to a positive effect on the visual quality of the fruit. The presence of high N 2 concentrations in the MAP, on the other hand, seems to have maintained the initial characteristics of the package by avoiding mechanical damage to the fruit and thus avoiding an acceleration of physiological senescence. The presence of N 2 in the bags makes it possible to reduce gas losses and keep the fruits qualitatively better [32]. Furthermore, as oxygen is the receptor for polyphenol oxidase, it appears that the treatment in MAP 3 inhibited its activation because the O 2 concentration within the bag was minimal (5%) initially compared to the other treatments and decreased during storage. However, no pulp decay phenomena were activated, as confirmed by the evaluation of the firmness descriptor in the sensory analysis. This phenomenon is probably because the pH remained above 4.5 and, thus, no acetaldehyde, the main contributor toward fermentation processes, was formed [22,71].
A study conducted by Jiang et al. [72] demonstrated that a brief exposure of litchi fruits to high concentrations of N 2 inhibits the production of ethanol in a nonsignificant way, while if the same treatment is carried out for 2, 4 or 6 days of storage, the significance of the application of the treatment increases. Similar results were obtained by storing apples and pears at low O 2 concentrations [73]. According to Saquet et al. [14], the browning of some fruits could be associated with the elevated concentration of ATP present in the tissues, with a consequent loss of the compartmentalization between the enzymes and their substrates, which causes enzymatic browning. Concerning the flesh quality, the more the surface color darkness, the more it will be characterized by a loss of weight and texture, with an associated loss of organoleptic and sensory quality [74]. Lowercase letters indicate significant differences (p < 0.05) between treatments on the same storage day; uppercase letters indicate significant differences (p < 0.05) for the same treatment throughout the storage period (d0 to d9).
Browning of the pericarp, as a marker of senescence, is a primary factor limiting the quality and shelf life of litchi fruits [9]. In the present study, the lowest O2 concentration in the MAP 3 package (5% O2 + 20% CO2 + 75% N2) would seem to relate back to a positive effect on the visual quality of the fruit. The presence of high N2 concentrations in the MAP, on the other hand, seems to have maintained the initial characteristics of the package by avoiding mechanical damage to the fruit and thus avoiding an acceleration of physiological senescence. The presence of N2 in the bags makes it possible to reduce gas losses and keep the fruits qualitatively better [32]. Furthermore, as oxygen is the receptor for polyphenol oxidase, it appears that the treatment in MAP 3 inhibited its activation because the O2 concentration within the bag was minimal (5%) initially compared to the other treatments and decreased during storage. However, no pulp decay phenomena were activated, as confirmed by the evaluation of the firmness descriptor in the sensory analysis. This phenomenon is probably because the pH remained above 4.5 and, thus, no acetaldehyde, the main contributor toward fermentation processes, was formed [22,71]. A study conducted by Jiang et al. [72] demonstrated that a brief exposure of litchi fruits to high concentrations of N2 inhibits the production of ethanol in a nonsignificant way, while if the same treatment is carried out for 2, 4 or 6 days of storage, the significance of the application of the treatment increases. Similar results were obtained by storing apples and pears at low O2 concentrations [73]. According to Saquet et al. [14], the browning of some fruits could be associated with the elevated concentration of ATP present in the tissues, with a consequent loss of the compartmentalization between the enzymes and their substrates, which causes enzymatic browning. Concerning the flesh quality, the more the surface color darkness, the more it will be characterized by a loss of weight and texture, with an associated loss of organoleptic and sensory quality [74]. Lowercase letters indicate significant differences (p < 0.05) between treatments on the same storage day; uppercase letters indicate significant differences (p < 0.05) for the same treatment throughout the storage period (d0 to d9).

Firmness
Generally, the loss of fruit firmness during the storage period occurs mainly due to the transformation of water-insoluble protopectins to water-soluble pectins [75], regardless of how they are processed. Furthermore, as reported by Sivakumar et al. [11], the loss of firmness in litchi fruits is often associated with water loss. However, during the cold-storage period, firmness decreased. This phenomenon, caused by normal physiological decay processes, showed significant differences (p < 0.05) between the treated and CTR fruits. As highlighted in Figure 4, the firmness of litchi fruits stored in MAP showed no statistically significant differences from the CTR up to 3 days of storage, whereas, starting from the 6th day of storage, substantial decay of this parameter was observed in the CTR fruits and in MAP 1 (10% O 2 + 20% CO 2 + 70% N 2 ) and MAP 3 (5% O 2 + 20% CO 2 + 75% N 2 ). In contrast, the treatment in MAP 2 (20% O 2 + 20% CO 2 + 60% N 2 ) maintained higher firmness values up to the 9th day of storage. In fact, the loss of firmness in litchi in MAP 2 was much slower than in the other treatments and the CTR fruits. The initial firmness (fresh fruit at day 0) was 49.05 N, and it decreased in the CTR by 57.6%, in MAP 1 by 51.6%, in MAP 2 by 32% and in MAP 3 by 52% after 9 days of storage. According to the data obtained, we can confirm that MAP has the advantage of reducing and preventing the loss of firmness and, therefore, weight loss by maintaining a higher RH around the fruit inside the bag that prevents water loss due to transpiration. Similar results were reported by Lemmer and Kruger [76]. Other authors reported that maintaining firmness at low temperatures slowed the respiration rate and organic acid losses during the storage period [61]. In the fruits considered, probably the presence of nitrogen inside the package of MAP-treated fruits inhibited the loss of liquid, maintaining a high firmness and making the fruit appreciable to the panelists (see sensory evaluation).
(fresh fruit at day 0) was 49.05 N, and it decreased in the CTR by 57.6%, in MAP 1 by 51.6%, in MAP 2 by 32% and in MAP 3 by 52% after 9 days of storage. According to the data obtained, we can confirm that MAP has the advantage of reducing and preventing the loss of firmness and, therefore, weight loss by maintaining a higher RH around the fruit inside the bag that prevents water loss due to transpiration. Similar results were reported by Lemmer and Kruger [76]. Other authors reported that maintaining firmness at low temperatures slowed the respiration rate and organic acid losses during the storage period [61]. In the fruits considered, probably the presence of nitrogen inside the package of MAP-treated fruits inhibited the loss of liquid, maintaining a high firmness and making the fruit appreciable to the panelists (see sensory evaluation). Lowercase letters indicate significant differences (p < 0.05) between treatments on the same storage day; uppercase letters indicate significant differences (p < 0.05) for the same treatment throughout the storage period (d0 to d9).

Chemical Composition
As shown in Table 1, the content of soluble solids (SSC) and the water content of litchi fruits decreased during storage according to other studies [74]. However, concerning the water content, just the CTR treatment shows significant differences (p < 0.05) between the treated fruit; from d0 to d9, it shows a water content loss from 83.87 ± 0.57 g/100 FW to 80.14 ± 0.07 g/100 FW with a percentage loss in the water content of 4.44%, while the other treatments show no significant differences. These results agree with what was found in the analysis of the evolution of firmness in litchi fruits. In samples with MAP, water loss was minor as the film acted as a barrier between the environment and the fruits. Moreover, fruits rich in MAP show a reduced loss of water, probably, also thanks to the concentration of CO2 inside the bags, which limits the evapotranspiration of the fruits, prolonging their shelf life. Similar results were found in other fruit packaged in a modified atmosphere, such as pomegranate [32,77], mango and pineapple [78], mulberry [33] and others. Moreover, litchi browning was well correlated with desiccation due to the lower RH around the fruit, which affects color retention, results in the loss of membrane permeability and increases browning [79]. Therefore, from the data obtained relating to the Lowercase letters indicate significant differences (p < 0.05) between treatments on the same storage day; uppercase letters indicate significant differences (p < 0.05) for the same treatment throughout the storage period (d0 to d9).

Chemical Composition
As shown in Table 1, the content of soluble solids (SSC) and the water content of litchi fruits decreased during storage according to other studies [74]. However, concerning the water content, just the CTR treatment shows significant differences (p < 0.05) between the treated fruit; from d0 to d9, it shows a water content loss from 83.87 ± 0.57 g/100 FW to 80.14 ± 0.07 g/100 FW with a percentage loss in the water content of 4.44%, while the other treatments show no significant differences. These results agree with what was found in the analysis of the evolution of firmness in litchi fruits. In samples with MAP, water loss was minor as the film acted as a barrier between the environment and the fruits. Moreover, fruits rich in MAP show a reduced loss of water, probably, also thanks to the concentration of CO 2 inside the bags, which limits the evapotranspiration of the fruits, prolonging their shelf life. Similar results were found in other fruit packaged in a modified atmosphere, such as pomegranate [32,77], mango and pineapple [78], mulberry [33] and others. Moreover, litchi browning was well correlated with desiccation due to the lower RH around the fruit, which affects color retention, results in the loss of membrane permeability and increases browning [79]. Therefore, from the data obtained relating to the evolution of browning and water content, we can state that the MAP technology was able to maintain a high RH by slowing down the senescence of the treated fruits in comparison with the CTR fruits. Concerning the soluble solids content, only MAP 2 (20% O 2 + 20% CO 2 + 60% N 2 ) remains unchanged during the storage period. Specifically, the SSC increases from day 6 of storage for the CTR, MAP 1 (10% O 2 + 20% CO 2 + 70% N 2 ) and MAP 3 (5% O 2 + 20% CO 2 + 75% N 2 ), while in MAP 2, it increases at day 3 of storage and then stabilizes. At day 9 of storage, there is an increase in soluble solids of 16.17% for the CTR, 19.24% for MAP 1, 14.70% for MAP 2 and 23.77% for MAP 3. The MAP 2 conditions were, therefore, more advantageous for the maintenance of the SSC, although an increase in the SSC concentration is physiological in litchi as it corresponds to the progression of ripening [80]. Finally, regarding the maturity index (MI), the CTR fruits experienced an increase (p < 0.05) in this index at as early as 3 days of storage (a 32.20% increase by the ninth day of storage), while for fruits stored in MAP, there seems to have been a slowdown in the rate of ripening, resulting in lower MI values. Specifically, the MAP treatments resulted in an increase in the maturity index of 14.61%, 18.42% and 20% in MAP 1, MAP 2 and MAP 3, respectively. Table 1. Change in water, soluble solids content and maturity index. The values indicate the means ± standard deviation (n = 5), and the * indicates the significant differences (p < 0.05) for the same treatment throughout the storage period (d0 to d9).

Organic Acids
As may be observed in Table 2, the fruits of all the treatments exhibit a reduction in organic acid content during the 9-day storage period, with no significant differences between treatments. Fumaric and malic acids are particularly present in litchi fruits during the entire observation period. The reduction in the concentration of organic acids as ripening progresses reflects the conversion of sugars into acids [81]. Fruit acidification was also observed in litchi stored at low temperatures for 3 weeks [82]. Further support comes from a study on litchi stored at 22 • C in paper or plastic bags [83]. It appears that as the litchi ripens, the sharp drop in sugars and the increase in acidity indicate an altered Krebs cycle function [81]. As reported by Somboonkaew and Terry [84], this could be because organic acids are an important source for general metabolism, including the respiratory cycle of tricarboxylic acid in harvested fruits. Finally, the maintenance of citric acids in the pulp of litchi fruits may be due to the protective O 2 barrier or the reduced oxygen supply on the fruit surface that inhibited respiration, both in the CTR fruits, stored in passive packaging, and in MAP-stored fruits [85]. Table 2. Concentration of organic acid (malic, fumaric and citric acids) during storage period (d0 to d9). The value indicates the means ± standard deviation (n = 5).

Proximate Composition
The analysis of the results regarding the proximate composition of litchi fruits, as can be seen from Table 3, shows a concentration of minerals in agreement with that reported by the USDA Nutrient [86]. In general, a decrease in the composition of K, Na, Ca, P and Fe is observed in all treatments and during the entire observation period (9 days). In particular, K undergoes a greater decrease in its concentration in the CTR-treated fruits than in the MAP-treated fruits. In contrast, the composition of the other minerals, Na, Ca, P and Fe, undergoes a decrease during the first 3 days of storage in all treatments and then stabilizes until the end of the observation period. These results confirm what has been reported in the literature, namely that litchi pulp is recommended as a good source of nutrients, such as vitamins and minerals [87]. Table 3. Concentration of mineral compounds (K, Na, Ca, P, Fe) during storage period. The values indicate the means ± standard deviation (n = 5). Lowercase letters indicate significant differences (p < 0.05) between treatments on the same storage day; uppercase letters indicate significant differences (p < 0.05) for the same treatment throughout the storage period (d0 to d9).

Vitamin Composition
Vitamin C content, as can be seen from Table 4, decreased more rapidly in the CTR fruits than in the other treatments (32%). Furthermore, no significant difference was found between the three MAP treatments (a 26%, 28% and 30% loss, respectively). Therefore, a significant difference was only found between the MAP and CTR treatments. This could be because the MAP treatment reduces cell degradation, maintains the fluids inside the fruit and delays all the senescence phenomena of the pulp, keeping inside the pericarp the organic acids, sugars, vitamins and proteins present in the fresh fruit [88]. As can be seen in Table 4, the CTR fruit underwent a much faster decrease in all the vitamin components analyzed, showing a significant difference between the MAP-treated and CTR fruit. The MAP-treated fruits, although they had a decreasing trend during the storage period, remained with higher values and, in the case of thiamine, even stabilized concentrations, especially in MAP 3 (5% O 2 + 20% CO 2 + 75% N 2 ), until the last day of storage (d9). The most unstable vitamin component, on the other hand, proved to be riboflavin, which suffered a significant peak loss in MAP 3 on the third day of storage, while the CTR did not differ from the MAP treatments throughout the storage period. Table 4. Concentration of vitaminic components (niacin, riboflavin, thiamine, ascorbic acid) during storage period. The values indicate the means ± standard deviation (n = 5). Lowercase letters indicate significant differences (p < 0.05) between treatments on the same storage day; uppercase letters indicate significant differences (p < 0.05) for the same treatment throughout the storage period (d0 to d9).

Microbiological Evolution during Refrigerated Storage
Litchi is a highly perishable fruit due to its susceptibility to different microbial infections determined by bacteria and unicellular and filamentous fungi [42,89]. Therefore, as with all horticultural products, the evaluation of the microbial composition of litchi fruits during cold storage deserves particular attention [90]. Pseudomonas and yeasts, responsible for the physicochemical modification and generation of off-odors and flavors of fruits and vegetables [91,92], were below the detection limit at each collection time. No colonies of Enterobacteriaceae, a family that includes inflammogenic enteric pathogens [93], were detected in any sample object of investigation. The results of the specific search for Escherichia coli, Listeria monocytogenes and Salmonella spp., responsible for food-borne diseases outbreaks [94], did not reveal any colony in either of the samples analyzed. Figure 5a shows the viable counts of TMMs and molds harbored on the CTR and MAP-treated litchi fruits during refrigerated storage. Generally, litchi fruits show an initial amount of TMMs and molds between 10 2 and 10 3 CFU/g [95], while in this study, none of the samples analyzed soon after production and after 3 d of refrigerate storage scored positively for the presence of these microbial groups. These results clearly indicated the respect of the food safety and hygiene criteria during the sanification and manipulation of litchi fruits. Significant differences (p < 0.05) were found from the third sampling time (6 d) for the levels of TMMs ( Figure 5a) and molds (Figure 5b) among the CTR and MAP-treated litchi fruits.
fruits during refrigerated storage. Generally, litchi fruits show an initial amount of TMMs and molds between 10 2 and 10 3 CFU/g [95], while in this study, none of the samples analyzed soon after production and after 3 d of refrigerate storage scored positively for the presence of these microbial groups. These results clearly indicated the respect of the food safety and hygiene criteria during the sanification and manipulation of litchi fruits. Significant differences (p < 0.05) were found from the third sampling time (6 d)  These microorganisms were found at about 10 5 CFU/g in the CTR production and reached levels of 10 6 CFU/g after 9 d of refrigerated storage, exceeding the threshold recommended for human consumption [96,97]. Interestingly, the development of TMMs and These microorganisms were found at about 10 5 CFU/g in the CTR production and reached levels of 10 6 CFU/g after 9 d of refrigerated storage, exceeding the threshold recommended for human consumption [96,97]. Interestingly, the development of TMMs and molds were inhibited in all MAP-treated litchi fruits, confirming the ability of this treatment to extend the shelf life of fresh food [98]. To our knowledge, no previous work has evaluated the microbiological aspects of MAP-treated litchi fruits, and for this purpose, any comparison with the literature data is not possible. However, the absence of TMMs and molds in all MAP samples can be explained by the presence in these packaging systems of 20% of CO 2 , which is able to inhibit microbial growth [99].

Sensory Analysis
A sensory evaluation showed that fresh litchi fruits (Figure 6a) were highly valued for FC, C, FO, EXO, FRF and EXF, reaching almost the maximum score (8). These values maintained a high score (7) even after 3 days of storage (Figure 6b) in the CTR and MAP 1 fruits (10% O 2 + 20% CO 2 + 70% N 2 ), while they decreased slightly in the MAP 2-(20% O 2 + 20% CO 2 + 60% N 2 ) and MAP 3-treated fruits (5% O 2 + 20% CO 2 + 75% N 2 ). No negative descriptors appear, however, that could be associated with the production of unpalatable compounds, such as acetaldehyde. Substantial differences begin to be seen from the sixth day of storage (Figure 6c) between the MAP-treated and CTR fruits. In fact, as we can see from Figure 6c, the MAP2-treated fruits maintain high values of positive descriptors of FC, FO, EXO, S and J. In contrast, the MAP 1-and MAP 3-treated fruits show slightly lower values of positive descriptors. Interestingly, starting from day 6, negative descriptors (OFF and PU) begin to appear in the CTR fruits compared to the MAP fruits in which these descriptors maintain values of 1 (absence of the descriptor). Finally, on the last day of analysis, the fruits (Figure 6d) with the best quality characteristics are those treated with MAP 3. In fact, these fruits maintain values of 7 in the descriptors C, FO, EXO, S and J. Similar values are also maintained by the MAP 1-and MAP 2-treated fruits, while the CTR fruits are evaluated negatively for the occurrence and persistence of the descriptors OFF, PU and A. As reported in other studies, the taste scale of the pulp and/or the color scale may reflect the quality of the litchi fruit, and, in addition, the decrease in the taste scale is well associated with the decrease in the color scale [100]. Therefore, from the data obtained, we can say that the MAP treatment maintained the quality attributes and prolonged shelf life. These beneficial effects should be mainly attributed to the protective effects of gases around the fruits that prevent browning, slow respiration and juice escape and slow down senescence. molds were inhibited in all MAP-treated litchi fruits, confirming the ability of this treatment to extend the shelf life of fresh food [98]. To our knowledge, no previous work has evaluated the microbiological aspects of MAP-treated litchi fruits, and for this purpose, any comparison with the literature data is not possible. However, the absence of TMMs and molds in all MAP samples can be explained by the presence in these packaging systems of 20% of CO2, which is able to inhibit microbial growth [99].

Sensory Analysis
A sensory evaluation showed that fresh litchi fruits (Figure 6a) were highly valued for FC, C, FO, EXO, FRF and EXF, reaching almost the maximum score (8). These values maintained a high score (7) even after 3 days of storage (Figure 6b) in the CTR and MAP 1 fruits (10% O2 + 20% CO2 + 70% N2), while they decreased slightly in the MAP 2-(20% O2 + 20% CO2 + 60% N2) and MAP 3-treated fruits (5% O2 + 20% CO2 + 75% N2). No negative descriptors appear, however, that could be associated with the production of unpalatable compounds, such as acetaldehyde. Substantial differences begin to be seen from the sixth day of storage (Figure 6c) between the MAP-treated and CTR fruits. In fact, as we can see from Figure 6c, the MAP2-treated fruits maintain high values of positive descriptors of FC, FO, EXO, S and J. In contrast, the MAP 1-and MAP 3-treated fruits show slightly lower values of positive descriptors. Interestingly, starting from day 6, negative descriptors (OFF and PU) begin to appear in the CTR fruits compared to the MAP fruits in which these descriptors maintain values of 1 (absence of the descriptor). Finally, on the last day of analysis, the fruits (Figure 6d) with the best quality characteristics are those treated with MAP 3. In fact, these fruits maintain values of 7 in the descriptors C, FO, EXO, S and J. Similar values are also maintained by the MAP 1-and MAP 2-treated fruits, while the CTR fruits are evaluated negatively for the occurrence and persistence of the descriptors OFF, PU and A. As reported in other studies, the taste scale of the pulp and/or the color scale may reflect the quality of the litchi fruit, and, in addition, the decrease in the taste scale is well associated with the decrease in the color scale [100]. Therefore, from the data obtained, we can say that the MAP treatment maintained the quality attributes and prolonged shelf life. These beneficial effects should be mainly attributed to the protective effects of gases around the fruits that prevent browning, slow respiration and juice escape and slow down senescence.

Conclusions
MAP has a positive effect on the containment of epicarp burnishing and loss of consistency. The MAP 1 and MAP 2 treatments extended the shelf life of the fruit up to nine days. The MAP 3 treatments (5% O2 + 20% CO2 + 75% N2) provided the best results in the control of decay and in the maintenance of the external and internal quality of the fruit. In fact, from a microbiological point of view, packaging litchi fruits in modified atmospheres containing 20% of CO2 is an optimal strategy to prevent microbial deterioration. As for quality traits, the skin color, brightness and redness were slowed down in the MAPtreated fruit for browning and loss of firmness. This could be due to the solubility in the water of N2, which is 0.016 g L −1 . The increased ability of MAP 3 to delay physiological decay may be due to the ability of nitrogen to dissolve in the aqueous layer of the fruit and, thus, inactivate some chemically active sites on the enzymes. Finally, the reduced level of oxygen in the MAP 3 mixture has certainly avoided the activation of anaerobic metabolism, maintained the organoleptic characteristics of litchi and prolonged its shelf life. Fruits treated with MAP show a reduced loss of water and maintained their SSC, with a lower ripening index compared to the CTR ones. Furthermore, the vitamin content generally decreased more rapidly in the CTR fruits than in the other treatments. Moreover, the MAP-treated fruits maintained the best values of sensory positive descriptors during storage. Finally, MAP treatment maintained the quality attributes and prolonged fruit shelf life. These beneficial effects should be mainly attributed to the protective effects of gases around the fruits that prevent browning, slow respiration and juice escape and slow down senescence. In particular, MAP 3 seems to maintain the better organoleptic quality of litchi fruits.

Conclusions
MAP has a positive effect on the containment of epicarp burnishing and loss of consistency. The MAP 1 and MAP 2 treatments extended the shelf life of the fruit up to nine days. The MAP 3 treatments (5% O 2 + 20% CO 2 + 75% N 2 ) provided the best results in the control of decay and in the maintenance of the external and internal quality of the fruit. In fact, from a microbiological point of view, packaging litchi fruits in modified atmospheres containing 20% of CO 2 is an optimal strategy to prevent microbial deterioration. As for quality traits, the skin color, brightness and redness were slowed down in the MAP-treated fruit for browning and loss of firmness. This could be due to the solubility in the water of N 2 , which is 0.016 g L −1 . The increased ability of MAP 3 to delay physiological decay may be due to the ability of nitrogen to dissolve in the aqueous layer of the fruit and, thus, inactivate some chemically active sites on the enzymes. Finally, the reduced level of oxygen in the MAP 3 mixture has certainly avoided the activation of anaerobic metabolism, maintained the organoleptic characteristics of litchi and prolonged its shelf life. Fruits treated with MAP show a reduced loss of water and maintained their SSC, with a lower ripening index compared to the CTR ones. Furthermore, the vitamin content generally decreased more rapidly in the CTR fruits than in the other treatments. Moreover, the MAP-treated fruits maintained the best values of sensory positive descriptors during storage. Finally, MAP treatment maintained the quality attributes and prolonged fruit shelf life. These beneficial effects should be mainly attributed to the protective effects of gases around the fruits that prevent browning, slow respiration and juice escape and slow down senescence. In particular, MAP 3 seems to maintain the better organoleptic quality of litchi fruits.

Data Availability Statement:
The data presented in this study are available on request from the corresponding author.