Characterization of a Galactose Oxidase from Fusarium odoratissimum and Its Application in the Modification of Agarose

A galactose oxidase gene, gao-5f, was cloned from Fusarium odoratissimum and successfully expressed in E. coli. The galactose oxidase GAO-5F belongs to the AA5 family and consists of 681 amino acids, with an estimated molecular weight of 72 kDa. GAO-5F exhibited maximum activity at 40 °C and pH 7.0 and showed no change in activity after 24 h incubation at 30 °C. Moreover, GAO-5F exhibited 40% of its maximum activity after 24 h incubation at 50 °C and 60% after 40 h incubation at pH 7.0. The measured thermostability of GAO-5F is superior to galactose oxidase’s reported thermostability. The enzyme exhibited strict substrate specificity toward D-galactose and oligosaccharides/polysaccharides containing D-galactose. Further analysis demonstrated that GAO-5F specifically oxidized agarose to a polyaldehyde-based polymer, which could be used as a polyaldehyde to crosslink with gelatin to form edible packaging films. To our knowledge, this is the first report about the modification of agarose by galactose oxidase, and this result has laid a foundation for the further development of edible membranes using agarose.


Introduction
Renewable resources are economical, green, abundant, and degradable, and are essential in developing the food industry, and packaging, biomedicine, and other fields [1]. The development of green and environmentally friendly packaging materials using renewable resources is of great significance to solve the environmental problems caused by petroleumbased plastics and to improve the quality of food [2]. Edible packaging film prepared from natural edible materials comes into being and has promising application prospects in the food industry [3,4]. Among them, edible film made from gelatin as the main raw material has the advantages of being green, non-toxic, non-hazardous, and biodegradable. Therefore, gelatin edible film is a new packaging material with great potential to replace petroleum-based plastic packaging materials [5]. However, the mechanical strength, flexibility, and hydrophobicity of single gelatin edible film have certain defects, which represent a bottleneck restricting its industrial production and commercial application, so there is an urgent need to improve the performance of gelatin edible film. One of the effective ways to improve the performance of gelatin edible films is to use an aldehyde-containing crosslinking agent for crosslinking modification, which can enhance the stability of the film structure and improve its overall performance [6,7]. By introducing crosslinking agents, a new three-dimensional grid structure can be formed within/between gelatin molecules, chemically characterized. The most commonly used method for galactose oxidase d mination is to monitor the production of H2O2, using 2,2'-Azino-bis (3-ethylbenz zoline-6-sulfonic acid) (ABTS) or o-dianisidine [25,34]. Considering the toxicity of t ABTS was employed in characterizing galactose oxidase. In addition, for the first tim galactose oxidase GAO-5F was used for oxidation of agarose to yield a polyaldeh based polymer, which can be further used to crosslink with gelatin to produce e packaging films.

Schematic Overview of the Experimental Program
In this study, novel galactose oxidases were mined from NCBI GeneBank. The t gene was expressed in E. coli successfully. The purified enzyme was characterized f biochemical properties. Following up on its oxidation properties, the polyaldehyde-b polymers were prepared using galactose oxidase-catalyzed oxidation with agarose a substrate. The schematic overview of the experimental program is shown in Figure 1

Materials, Strains and Culture Conditions
D-galactose was purchased from Sinopharm Chemical Reagent Co., Ltd. (Be China). ABTS and horseradish peroxidase were purchased from MACKLIN (Shan China). All other chemicals and reagents used were of analytical grade and were chased from local markets. SDS-PAGE protein standard was from BioRad (Herts, U coli BL21 Star™ (DE3) strains (Tiangen Biotech, Beijing, China) were used as the pro otic expression host.

Materials, Strains and Culture Conditions
D-galactose was purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). ABTS and horseradish peroxidase were purchased from MACKLIN (Shanghai, China). All other chemicals and reagents used were of analytical grade and were purchased from local markets. SDS-PAGE protein standard was from BioRad (Herts, UK). E. coli BL21 Star™ (DE3) strains (Tiangen Biotech, Beijing, China) were used as the prokaryotic expression host.

Expression of the Three Galactose Oxidase Genes in E. coli
To explore their potential catalytic performance, the above three optimized codon genes, gao-1f, gao-5f, and gao-13f, encoding the galactose oxidases GAO-1F, GAO-5F, and GAO-13F without signal peptides were synthesized by Sangon Biotech (Shanghai, China). The genes were ligated to the pET-28a(+) vector, respectively. The resulting plasmids pET-28a-GAO-1F, pET-28a-GAO-5F, and pET-28a-GAO-13F were then transformed into E. coli BL21 (DE3) competent cells for expression of the galactose oxidases. The constructed plasmids and their corresponding recombinant strains were stored at −80 • C. Recombinant E. coli BL21 (DE3) cells harboring galactose oxidase genes in the pET-28a(+) vector, including GAO-1F, GAO-5F, and GAO-13F, were cultivated in LB medium containing kanamycin (50 µg/mL) at 37 • C for~3 h with orbital shaking at 200 rpm. When the absorbance at 600 nm of the culture broth reached 0.6, overexpression of protein was induced by adding IPTG at a final concentration of 0.1 mM. The induced cultures were further incubated for 18 h at 25 • C (200 rpm). Then, cells were collected by centrifugation at 4 • C (8000× g) for 10 min, washed twice with 0.85% NaCl solution, and stored at −20 • C.

Purification of the Three Galactose Oxidases
The recombinant proteins were purified by nickel affinity chromatography. Firstly, the harvested cells were resuspended in Tris-HCl buffer (50 mM, pH 8.0), then disrupted by ultrasonication. The cell debris was removed by centrifugation (10,000× g, 4 • C) for 30 min. The supernatants containing the crude enzymes were filtered through a 0.45 µm filter and then loaded onto a Ni-NTA column (1 mL; Qiagen, Hilden, Germany) at a flow rate of 1 mL/min. The column was preequilibrated with buffer A (50 mM NaH 2 PO 4 , 300 mM NaCl, and 10 mM imidazole, pH 8.0). Then, the column was washed with 5 column volumes of buffer B (50 mM NaH 2 PO 4 , 300 mM NaCl, and 20 mM imidazole, pH 8.0), followed by 5 column volumes of buffer C (50 mM NaH 2 PO 4 , 300 mM NaCl, and 50 mM imidazole, pH 8.0), 10 column volumes of buffer D (50 mM NaH 2 PO 4 , 300 mM NaCl, and 150 mM imidazole, pH 8.0) and 5 column volumes of buffer E (50 mM NaH 2 PO 4 , 300 mM NaCl, and 200 mM imidazole, pH 8.0) at a flow rate of 1 mL/min. The eluted proteins were collected respectively, then desalted with Tris-HCl buffer (50 mM, pH 8.0) and concentrated by ultrafiltration using a 50 mL Amicon Ultra Centrifugal Filter Device with a molecular weight cutoff of 30 kDa (Millipore, Burlington, MA, USA) for subsequent experiments. The supernatants of the cell lysates and purified proteins were analyzed by 12.5% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Protein concentration was determined using the Bradford Protein Assay Kit purchased from Tiangen Biotech (Beijing, China), using bovine serum albumin as the standard. All purification steps were carried out at 4 • C.

Enzyme Assay
The enzymatic activity of galactose oxidase was determined by the ABTS indirect detection method using a peroxidase coupling assay [37]. Galactose oxidase catalyzes the oxidation of primary alcohols to corresponding aldehydes and reduces oxygen to hydrogen peroxide in the catalytic reaction. ABTS, as a color developing agent, can be oxidized by hydrogen peroxide to produce blue-green cationic free radicals with maximum absorption at 420 nm. The standard assays were performed in a reaction mixture (2.7 mL) containing ABTS (4.41 mg) in phosphate buffer (100 mM, pH 7.0), horseradish peroxidase (45 U), D-galactose (90 mM), NaCl (150 mM), and 10 µL purified galactose oxidase. The mixture was incubated at 40 • C for 3 min and then the absorbance at 420 nm was measured. All the reactions were performed in triplicate and their mean and standard deviation values were used as the final results. One unit (U) of galactose oxidase activity was defined as the amount of enzyme required to oxidize 2 µmol ABTS or 1 µmol D-galactose per minute under the above conditions, which is equal to 1 µmol O 2 consumed per min.

Enzyme Characterization
D-galactose (90 mM) was used as the substrate in the enzymatic characterization of GAO-5F. The optimal temperature for GAO-5F activity was determined in 20 mM phosphate buffer (pH 7.0) over the temperature range of 20-60 • C. Different buffer solutions, including sodium citrate buffer (20 mM, pH 3.0 to 6.0), phosphate buffer (20 mM, pH 6.0 to 8.0), Tris-HCl buffer (20 mM, pH 8.0 to 9.0), and glycine-NaOH buffer (20 mM, pH 9.0 to 10.0) were used to determine the optimum reaction pH of GAO-5F. The relative activities under optimal temperature and pH conditions were defined as 100%.
The thermal stability of purified GAO-5F was determined to test the residual enzyme activity by incubating the enzyme at 30, 40, and 50 • C for different times. GAO-5F was incubated in phosphate buffer (pH 6.0 to 8.0) at 40 • C for several hours, and then the pH stability was measured in 20 mM phosphate buffer (pH 7.0).
The kinetic parameters of GAO-5F toward D-galactose with different concentrations (30 to 300 mM) were determined. The reaction mixture was incubated at 40 • C for 3 min, and the change in absorbance was detected by a spectrophotometer. K m and V max of the purified GAO-5F were calculated by nonlinear regression analysis and the Michaelis-Menten equation using Origin 9.0 (OriginLab, Northampton, MA, USA).
The effects of different metal ions and chemicals on recombinant GAO-5F activities were determined by pre-incubating the enzyme in 20 mM phosphate buffer (pH 7.0) with a series of metal ions, including Na + , K + , Mg 2+ and Cu 2+ , and chemicals, including EDTA, hexadecyl trimethyl ammonium bromide (CTAB), SDS, and Tween 80. Since Cu 2+ precipitates in phosphate buffer (pH 7.0) to form insoluble copper phosphate, the phosphate buffer was replaced with ddH 2 O (pH 7.0) in the determination of the effect of Cu 2+ on the activity of GAO-5F. All compounds except Tween 80 (2.5%) were added to a final concentration of 5 mM. The catalytic activity without added metal ions was used as the control, and the activity was defined as 100%.

Oxidation of Agarose by GAO-5F
The reaction mixture was composed of phosphate buffer (20 mM, pH 7.0), 15 mg agarose (boiled for 10 min to dissolve), and 50 U purified enzyme of GAO-5F. The mixture was incubated at 40 • C for 30 min and then kept at 4 • C for 1 h. The unreacted substrate agarose gel was removed by centrifugation at 8000× g for 10 min. The reaction product and the substrate were analyzed by Fourier Transform Infrared (FTIR) spectrometry (Ther-moScientific Nicolet IS5, Waltham, MA, USA) against the background of a blank KBr pellet. The spectra were produced with a wave number range from 4000 to 400 cm -1 and at a resolution of 4 cm -1 over 32 cumulative scans.

Sequences Analysis
Based on the GenBank database mining, three galactose oxidase genes, gao-1f, gao-5f , and gao-13f from F. oxysporum, F. odoratissimum, and F. flagelliforme, respectively, were selected from the NCBI database. The full-length GAO-1F, GAO-5F, and GAO-13F genes had open reading frames of 2040, 2046, and 2043 bp encoding 680, 682, and 681 amino acids, including predicted signal peptides of 20, 26, and 16 amino acids, respectively. All of the three galactose oxidases without signal peptides were synthesized with codon optimization. Multiple sequence alignment with other reported galactose oxidases from Fusarium species was conducted, and the results showed that their catalytic domains contained four key active sites (Y308, Y535, H536, and H621), which are conserved in all selected galactose oxidases (Figure 2A) [38]. To gain a better understanding of the relationships involved in the molecular evolution of these galactose oxidases, a phylogenetic tree comprising GAO-1F (ENH75387.1), GAO-5F (XP_031061741.1), and GAO-13F (RFN47484.1) with other reported Fusarium-derived galactose oxidases was constructed ( Figure 2B). The results showed that most of them exhibited high homology except GAO-1F, DAA34004.1, and ADG08187.1. The catalytic activity of DAA34004.1 and ADG08187.1 has not been reported. The galactose oxidase activities of GaoA (GenBank AJE27923.1) from F. subglutinans and GalOx (GenBank AHA90705.1) from F. oxysporum, which showed the highest homologous with GAO-5F, were 1255 U/mg (with a production of 1.38 mg/L of culture liquid medium) and 65.4 U/mg (with a production of 5.7 mg/L of soluble protein), respectively [33,34]. GAO-13F showed the highest identities with galactose oxidases GaoA from F. sambucinum (AIR07394.1) [32], and GAOX from F. oxysporum (AAA16228.1) [39]. The catalytic activity of GaoA was 159 U/mg [32], and the quality and yield of GAOX reached 500 mg/L with optimization of culture conditions [39].

Enzyme Expression, Purification, and Molecular Properties
The recombinant plasmids pET-28a-GAO-1F, pET-28a-GAO-5F, and pET-28a-GAO-13F with a 6×His tag at the N-terminus were constructed by gene synthesis, and these plasmids were successfully transformed into E. coli BL21(DE3) cells for expression. The three proteins were successfully expressed and purified to electrophoretic homogeneity using a Ni-NTA column. Three single clear bands with a molecular mass of approximately 72 kDa were visible on SDS-PAGE (Figure 3), and were seemingly consistent with those predicted by the deduced amino acid sequences. Most reported galactose oxidases from Fusarium species have molecular weights about 70 kDa, such as GalOx from F. sambucinum (68.5 kDa) [32], and GalOx from F. oxysporum (70 kDa) [33]. The enzyme activities of the purified enzymes were detected, and the specific activities of GAO-5F and GAO-13F were 62.837 U/mg and 38.636 U/mg, respectively, whereas GAO-1F was inactive (Table 1). Thus, GAO-5F was selected for further characterization.

Enzyme Expression, Purification, and Molecular Properties
The recombinant plasmids pET-28a-GAO-1F, pET-28a-GAO-5F, and pET-28a-GAO-13F with a 6×His tag at the N-terminus were constructed by gene synthesis, and these plasmids were successfully transformed into E. coli BL21(DE3) cells for expression. The three proteins were successfully expressed and purified to electrophoretic homogeneity using a Ni-NTA column. Three single clear bands with a molecular mass of approximately 72 kDa were visible on SDS-PAGE (Figure 3), and were seemingly consistent with those predicted by the deduced amino acid sequences. Most reported galactose oxidases from Fusarium species have molecular weights about 70 kDa, such as GalOx from F. sambucinum (68.5 kDa) [32], and GalOx from F. oxysporum (70 kDa) [33]. The enzyme activities of the purified enzymes were detected, and the specific activities of GAO-5F and GAO-13F were 62.837 U/mg and 38.636 U/mg, respectively, whereas GAO-1F was inactive (Table 1). Thus, GAO-5F was selected for further characterization.

Characterization of GAO-5F
The oxidative activity of purified GAO-5F was characterized using D-galactose as the substrate. The optimum reaction temperature of purified GAO-5F was at 40 • C, and it exhibited about 90% of the maximal activity at 60 • C ( Figure 4A). The optimal galactose oxidase activity of GAO-5F occurred at pH 7.0 in a 20 mM phosphate buffer, and it showed more than 80% of the maximal activity at pH 6.0-9.0 ( Figure 4B). This result is similar to other galactose oxidases. For example, the optimal activities of galactose oxidases from F. subglutinans, F. sambucinum, F. oxysporum, and F. acuminatum were observed at 30, 35, 40, and 30 • C, and pH values of 7.0, 6.0-7.5, 7.0, and 7.0, respectively [32][33][34]40]. The thermal stability of GAO-5F decreased with increasing temperature. No significant decrease in enzyme activity was observed when GAO-5F was stored at 30 • C for 25 h. Up to 40% of the enzyme activity was retained when GAO-5F was stored at 50 • C for 25 h ( Figure 4C). The measured thermostability is superior to those galactose oxidases reported from other Fusarium strains. For example, the galactose oxidase from F. oxysporum lost activity at less than 5 h of incubation at 50 • C [33]. The relative activity of galactose oxidase from F. subglutinans decreased to about 20% after 10 min incubation at 50 • C [34]. The properties of high specific activity at high temperatures and appropriate thermostability may be beneficial to the modification of agarose, the gelling temperature of which was usually at 35-40 • C. GAO-5F showed the greatest stability at pH 7.0, with more than 60% activity  Figure 4D). However, there was a dramatic decrease in activity after incubation at pH 6.0 and 8.0, and they retained less than 50% activity after 40 h. This result was similar to the galactose oxidase from F. subglutinans, which retained 60% activity after 48 h of incubation [34].

Characterization of GAO-5F
The oxidative activity of purified GAO-5F was characterized using D-galactose as the substrate. The optimum reaction temperature of purified GAO-5F was at 40 °C, and it exhibited about 90% of the maximal activity at 60 °C ( Figure 4A). The optimal galactose oxidase activity of GAO-5F occurred at pH 7.0 in a 20 mM phosphate buffer, and it showed more than 80% of the maximal activity at pH 6.0-9.0 ( Figure 4B). This result is similar to other galactose oxidases. For example, the optimal activities of galactose oxidases from F. subglutinans, F. sambucinum, F. oxysporum, and F. acuminatum were observed at 30, 35, 40, and 30 °C, and pH values of 7.0, 6.0-7.5, 7.0, and 7.0, respectively [32][33][34]40]. The thermal stability of GAO-5F decreased with increasing temperature. No significant decrease in enzyme activity was observed when GAO-5F was stored at 30 °C for 25 h. Up to 40% of the enzyme activity was retained when GAO-5F was stored at 50 °C for 25 h ( Figure 4C). The measured thermostability is superior to those galactose oxidases reported from other Fusarium strains. For example, the galactose oxidase from F. oxysporum lost activity at less than 5 h of incubation at 50 °C [33]. The relative activity of galactose oxidase from F. subglutinans decreased to about 20% after 10 min incubation at 50 °C [34]. The properties of high specific activity at high temperatures and appropriate thermostability may be beneficial to the modification of agarose, the gelling temperature of which was usually at 35-40 °C. GAO-5F showed the greatest stability at pH 7.0, with more than 60% activity retained after 40 h incubation ( Figure 4D). However, there was a dramatic decrease in activity after incubation at pH 6.0 and 8.0, and they retained less than 50% activity after 40 h. This result was similar to the galactose oxidase from F. subglutinans, which retained 60% activity after 48 h of incubation [34].   Kinetic measurements were determined under standard reaction conditions using Dgalactose as substrate. The Km, Vmax, kcat, and kcat/Km of purified GAO-5F were determined to be 31.9 mM, 0.1636 μmol/min/mg, 129.1366 s -1 , and 4.04 mM -1 s -1 , respectively. The af- Kinetic measurements were determined under standard reaction conditions using D-galactose as substrate. The K m , V max , k cat , and k cat /K m of purified GAO-5F were determined to be 31.9 mM, 0.1636 µmol/min/mg, 129.1366 s -1 , and 4.04 mM -1 s -1 , respectively. The affinity and catalytic efficiency of GAO-5F were moderate when compared with those values for reported galactose oxidases. The K m of GAO-5F was higher than the galactose oxidase from F. acuminatum (16.2 mM) [40], whereas it was lower than those from F. oxysporum (47 mM) [32] and F. sambucinum (61 mM) [33]. The k cat /K m of GAO-5F was higher than galactose oxidases from F. sambucinum (0.89 mM -1 s -1 ) [33] and F. oxysporum (2 mM -1 s -1 ) [32], but lower than that from F. subglutinans (92 mM -1 s -1 ) [34].
The effects of different metal ions and compounds on the activity of GAO-5F were analyzed, and the results are shown in Table 2. Monovalent and divalent cations, such as Na + , K + , and Mg 2+ , inhibited enzyme activity to a minor extent, whereas Cu 2+ enhanced enzyme activity by 1.42-fold. This observation is not unexpected because galactose oxidase is a copper-dependent enzyme [41]. As a chelator of metal ions, EDTA caused a clear decrease in the activity of GAO-5F, which indicated that the enzyme was a metalloenzyme and required Cu 2+ as a cofactor to perform its oxidative function. This result is similar to published data for galactose oxidases from F. sambucinum [32]. CTAB had a significant inhibitory effect on the enzyme, which showed only 14% relative activity. Tween 80 was shown previously to improve the absorbance readings of the galactose oxidase reaction, and the results herein showed that the activity of GAO-5F increased in the presence of Tween 80 [40]. Galactose oxidase has broad substrate specificity along with strict regioselectivity, which is one of the most interesting characteristics of this enzyme. Galactose oxidase accepts a variety of primary alcohols, such as benzyl alcohol, glycerol, and oligosaccharides or polysaccharides (non-reducing end containing D-galactose). The activity of purified GAO-5F toward various substrates was determined and the results were shown in Table 3. It showed the highest activity with D-galactose and more than 95% relative activity with methyl-α-D-galactoside and D-(+)-raffinose. In addition, it also displayed approximately 60% relative activity toward D-(+)-melibiose and relatively low activities (about 4%) with lactose monohydrate. The results were similar to those of other Fusarium sources of galactose oxidases [33,40]. GAO-5F exhibited catalytic activity toward D-galactose but D-glucose, sucrose, and maltose were not its substrates, which verified the strict regioselectivity of the galactose oxidase. GAO-5F also showed activity against marine polysaccharides containing D-galactose, such as agarose and κ-carrageenan, which offers significant potential in modifying marine polysaccharides. However, it showed no activity toward other tested polysaccharides, including soluble starch, fructooligosaccharides, guar gum, and gum arabic. In addition, the activity toward agarose was relatively low and there is huge distance for improvement by protein engineering in the future.

Oxidation of Agarose by GAO-5F
FTIR spectroscopy is used to examine molecular structures and chemical bonds, which enables the characterization and identification of compounds according to their unique structural features [42,43]. Infrared spectroscopy has been applied successfully to the structural analysis of agarose [44]. Figure 5 shows the FTIR spectra of the substrate agarose (S1) and the polyaldehyde-based polymer (D1) oxidized by GAO-5F. The FTIR spectrum of S1 is similar to the reported data [45], with the absorption peak at 3385.75 cm -1 representing the -OH bond stretching vibration, which is the characteristic peak of polysaccharides. The absorption peak at 2952 cm -1 represents the stretching vibration of the intra-ring C-H, whereas the peak at 1385 cm -1 represents the methyl group. The absorption peaks observed at 1248 cm -1 , 1154 cm -1 , and 1049 cm -1 indicate the presence of C-OH and C-O-C. The FTIR spectra of S1 and D1 were compared. In addition to the absorption peaks described above, D1 displayed a peak at 1735 cm -1 , which corresponds to the characteristic absorption peak of an aldehyde group through the oxidation process and was not observed in the spectra of S1 [46]. Therefore, the absorption peak at 1735 cm -1 indicated the generation of polyaldehyde-based polymers in the D1 sample. This result proved that the galactose oxidase GAO-5F catalyzes the generation of a polyaldehyde-based polymer from agarose, which may be suitable for use as a crosslinking agent to form edible packaging films.

Oxidation of Agarose by GAO-5F
FTIR spectroscopy is used to examine molecular structures and chemical bonds, which enables the characterization and identification of compounds according to their unique structural features [42,43]. Infrared spectroscopy has been applied successfully to the structural analysis of agarose [44]. Figure 5 shows the FTIR spectra of the substrate agarose (S1) and the polyaldehyde-based polymer (D1) oxidized by GAO-5F. The FTIR spectrum of S1 is similar to the reported data [45], with the absorption peak at 3385.75 cm -1 representing the -OH bond stretching vibration, which is the characteristic peak of polysaccharides. The absorption peak at 2952 cm -1 represents the stretching vibration of the intra-ring C-H, whereas the peak at 1385 cm -1 represents the methyl group. The absorption peaks observed at 1248 cm -1 , 1154 cm -1 , and 1049 cm -1 indicate the presence of C-OH and C-O-C. The FTIR spectra of S1 and D1 were compared. In addition to the absorption peaks described above, D1 displayed a peak at 1735 cm -1 , which corresponds to the characteristic absorption peak of an aldehyde group through the oxidation process and was not observed in the spectra of S1 [46]. Therefore, the absorption peak at 1735 cm -1 indicated the generation of polyaldehyde-based polymers in the D1 sample. This result proved that the galactose oxidase GAO-5F catalyzes the generation of a polyaldehydebased polymer from agarose, which may be suitable for use as a crosslinking agent to form edible packaging films. Figure 5. FTIR spectra of the substrate agarose (S1) and the polyaldehyde-based polymer (D1) oxidized by GAO-5F.

Conclusions
In this study, a galactose oxidase gene, gao-5f, from F. odoratissimum was cloned and heterologously expressed in E. coli. The biochemical properties of the purified recombinant GAO-5F were investigated in detail, revealing the highest activity toward D-galac- Figure 5. FTIR spectra of the substrate agarose (S1) and the polyaldehyde-based polymer (D1) oxidized by GAO-5F.

Conclusions
In this study, a galactose oxidase gene, gao-5f, from F. odoratissimum was cloned and heterologously expressed in E. coli. The biochemical properties of the purified recombinant GAO-5F were investigated in detail, revealing the highest activity toward D-galactose at pH 7.0 and 40 • C. In addition, GAO-5F displayed excellent thermal stability. Results from FTIR data showed that GAO-5F successfully catalyzed the oxidation of agarose to produce a polyaldehyde-based polymer. This result indicates the potential applicability of galactose oxidase in the field of edible packaging films. Combined with the strict regioselectivity, galactose oxidase has significant development prospects for potential industrial applications.