Variation in Mating Dynamics across Five Species of Leiobunine Harvestmen (Arachnida: Opliones)

The study of mating choices often focuses on correlates of traits to the overall outcome of a mating interaction. However, mating interactions can proceed through a series of stages, with opportunities for assessment at each stage. We compared whether male or female size predicted mating interaction outcome across several stages of mating in five species of North American leiobunine harvestmen (commonly known as daddy longlegs). Leiobunine harvestmen have been previously shown to exhibit incredible morphological diversity consistent with a spectrum of male–female antagonism. Across all of the species, we found a general progression of female size predicting the outcome (success and timing) of early stages of interactions, and male size or male size relative to female size predicting the outcome and timing of later stages of interactions. We also found that size was not a strong predictor of outcome in the two species on the lower end of the antagonism spectrum. The variation in how female and male size predicted outcomes across species and stages of mating suggests that multiple mechanisms may operate to shape mating dynamics within and across species. Given the close relatedness of the species studied, the patterns we uncovered suggest a rapid evolution of the traits and processes predicting the outcome of mating interactions.


Introduction
The extravagance and rapid evolution of sexual traits have captured the interest of biologists dating back to Darwin [1]. Behavioral and morphological traits used to coordinate sex are often the most divergent aspects of animal phenotype across populations and species [2][3][4][5][6][7][8]. One factor contributing to this extreme diversity is the range of sources of selection that can shape reproductive traits, including precopulatory choice [9], conflict over mating [10,11], pericopulatory choice, postcopulatory choice [2,12], and sperm competition [13,14]. These various sources of selection can differ across species, but they can also operate simultaneously within a single species [15].
Also contributing to the diversity of behaviors involved in mating is the exchange of information between males and females as they assess each other [4,[16][17][18]. These male-female interactions can occur over a series of mating stages-roughly broken into precopulatory, copulatory, and postcopulatory-with females and/or males assessing different traits at each stage to determine the amount of time or resources that they will continue to invest in the interaction. As a result, the sources of selection shaping sexual traits can vary across the stages of mating interactions and shape different traits [9,[18][19][20]. Across different stages of mating, the selection of behavioral and  Timeline of behaviors during precopulatory, copulatory, and postcopulatory stages of mating interactions in leiobunine harvestmen. All of the behaviors measured are indicated in the order in which they occur on the timeline. The outcomes of each stage include: male attempt, yes or no (y/n); female resistance, yes or no; intromission (mating), yes or no; and postcopulatory guarding, yes or no. . We identified species during collection using external morphological traits, and confirmed proper identification by examining species-specific genitalic traits [23,26]. Each individual was used in only a single mating trial. The mating history of individuals was unknown, but leiobunine harvestmen mate multiply (Fowler-Finn unpubl. [27]), and the individuals of all of the species have similarly unknown mating histories. However, we consider the potential for experience to confound results.

Study organisms-We
We housed the animals in individual containers for one day to three weeks prior to using them in a mating trial. The containers were deli dishes (dimensions: 11-cm diameter × 8-cm depth) with holes in the lids and mosquito netting stretched across the top to allow for air flow and a substrate on which the animals could climb. The exception to this setup was L. politum at Mountain Lake Timeline of behaviors during precopulatory, copulatory, and postcopulatory stages of mating interactions in leiobunine harvestmen. All of the behaviors measured are indicated in the order in which they occur on the timeline. The outcomes of each stage include: male attempt, yes or no (y/n); female resistance, yes or no; intromission (mating), yes or no; and postcopulatory guarding, yes or no.

Materials and Methods
Study organisms-We collected the animals as mature individuals from four locations (year and GPS coordinates in parentheses): . We identified species during collection using external morphological traits, and confirmed proper identification by examining species-specific genitalic traits [23,26]. Each individual was used in only a single mating trial. The mating history of individuals was unknown, but leiobunine harvestmen mate multiply (Fowler-Finn unpubl. [27]), and the individuals of all of the species have similarly unknown mating histories. However, we consider the potential for experience to confound results.
We housed the animals in individual containers for one day to three weeks prior to using them in a mating trial. The containers were deli dishes (dimensions: 11-cm diameter × 8-cm depth) with holes in the lids and mosquito netting stretched across the top to allow for air flow and a substrate on which the animals could climb. The exception to this setup was L. politum at Mountain Lake Biological Station, for which the containers were 10 cm × 10 cm × 5 cm plastic containers, which is the best approximation available at the station. We fed all of the animals upon arrival in the lab, provided water ad libitum, and cleaned cages and freshened food (consisting of fish food and varied leftovers) and water twice weekly.
Mating trials-We conducted all of the mating trials in circular arenas (30-cm diameter) constructed from 22-cm high acetate walls and printer paper flooring [18]. To provide a backdrop for viewing videos, we surrounded the arena with white paper that was propped up~10 cm from the arena walls ( Figure 1). Between trials, we changed the paper on the floor, and wiped down the table and acetate paper with ethanol to remove potential chemical cues.
Prior to a trial, we gently placed individuals into the arena and placed a~five cm diameter acetate barrier around the individuals for a two minute acclimation period (except in 2013, when males were allowed to roam the arena during acclimation, but females were contained). Trials started when we lifted the barriers and allowed individuals to freely interact, and ended when either mating was complete with no further attempts to remate, or when the female rejected the male three times (following Fowler-Finn et al. 2014 [18]). We video recorded all of the trials using handheld digital camcorders for later behavioral analyses.
Following Fowler-Finn et al. (2014) [18], we examined a standard set of behaviors in each trial: if an attempt occurred or not, if the attempt was successful or not (on the first try, as well as overall in the trial), if the female resisted the first attempt by a male, and if copulation occurred or not. Resistance included one of the following behaviors, defined in Fowler-Finn et al. (2014) [18]: the female runs away, vigorously bobs her body, bites the male, or orients her body in a head-down position to block male access to clasping her in the mating embrace. We also recorded if guarding occurred, which was counted when males stayed in contact with the female for five seconds or longer after the end of copulation. Finally, we also quantified the length of an attempt to secure the female in the mating embrace, the length of intromission, and the length of postcopulatory contact. See Fowler-Finn et al. (2014) [18] for detailed descriptions of the behaviors, and Figure 2 for a mating interaction timeline.
Morphological analyses-We weighed individuals to the nearest 0.0001 g using a Mettler Toledo analytical balance at the end of each day. The timing of weighing allowed us to avoid disrupting the mating trials in the cases in which the animals released highly volatile alarm pheromones during weighing. All of the specimens were preserved in 70% ethanol at the conclusion of each experiment for morphological analyses. We measured the cephalothorax width at the widest point on the carapace between legs two and three as a measure of body size ( Figure 3A). To do so, we oriented individuals in a standardized position under a Leica 205 C microscope fitted with a Leica MC170 HD microscope camera at 4× magnification (Saint Louis University, Saint Louis, MO, USA) or Olympus SZX10 microscope (L. ventricosum Macalester College, St Paul, MN, USA). Images were captured and later measured using Leica imaging software. We took two pictures per individual-removing and then reorienting the body in between photographs-and measured both images twice. The final cephalothorax width measurement was the mean of means of these photographs.
We measured male pedipalp femur length because males secure females in a mating embrace by hooking their pedipalps behind the female's coxae of her second legs [18]. Furthermore, pedipalps have been shown to influence mating dynamics in L. vittatum [18], and also vary significantly across the antagonism spectrum, with larger pedipalps on the high antagonism end [23]. To do so, we first removed the right pedipalp (or the left when the right was damaged) and laid it in a stereotyped manner on a slide covered with a cover slip. We used the same microscope, camera, and image-processing software as we did for our body size measurements. Femur length was measured at the longest point-to-point distance diagonally across the pedipalp femur ( Figure 3B-D). Similarly as to body size, we took two pictures per pedipalp, repositioning the pedipalp in between photos, and used the mean of means for the final measurement. All of the researchers who took and processed images were trained in the same way by KD Fowler-Finn. Each researcher took two pictures and two measurements of each body part for 10 animals. Then, the repeatability of these measurements was verified with the variance component for individual identification (ID) in a mixed model analysis (p > 0.05, r > 0.98 for all datasets).
A single measure of 'body size' was calculated from the combination of weight and cephalothorax width. To do so, we ran a principal components analysis with weight and cephalothorax width, and retained the principal components with eigenvectors greater than 1.0. For each dataset, we identified only a single eigenvector explaining variation in body size for both males and females. We then used Pearson product moment correlations to determine if pedipalp length correlated with our measure of body size. Finally, we calculated sexual size dimorphism for each species for weight and cephalothorax width by dividing the male mean weight by female mean weight, and the male mean cephalothorax width by female mean cephalothorax width.
Statistical analyses-To determine the morphological predictors of each stage of mating for each dataset, we used nominal logistic regressions. Dependent variables included whether or not: the trial ended in mating (mate y/n), the male attempted (attempt y/n), the female resisted (resist y/n), the male secured the female on his first attempt (first attempt successful y/n), and the male eventually secured the female within three attempts (success y/n) ( Figure 2). The dependent variable was whether the pair successfully moved to the next stage of the mating sequence or not. The independent variables were female size, male size, the interaction between female size and male size, and male pedipalpal femur length. To determine whether the morphological traits predicted the length of copulation and the length of postcopulatory contact, we used linear regressions with the same independent variables.
Finally, following Fowler-Finn et al. (2014) [18], we looked at whether the relationship between the male-female size difference and attempt length differed depending on whether the attempt was successful or not. For all but L. calcar (which had a normal distribution), we log-transformed attempt length as the dependent variable. Independent variables were whether or not the attempt was All of the researchers who took and processed images were trained in the same way by KD Fowler-Finn. Each researcher took two pictures and two measurements of each body part for 10 animals. Then, the repeatability of these measurements was verified with the variance component for individual identification (ID) in a mixed model analysis (p > 0.05, r > 0.98 for all datasets).
A single measure of 'body size' was calculated from the combination of weight and cephalothorax width. To do so, we ran a principal components analysis with weight and cephalothorax width, and retained the principal components with eigenvectors greater than 1.0. For each dataset, we identified only a single eigenvector explaining variation in body size for both males and females. We then used Pearson product moment correlations to determine if pedipalp length correlated with our measure of body size. Finally, we calculated sexual size dimorphism for each species for weight and cephalothorax width by dividing the male mean weight by female mean weight, and the male mean cephalothorax width by female mean cephalothorax width.
Statistical analyses-To determine the morphological predictors of each stage of mating for each dataset, we used nominal logistic regressions. Dependent variables included whether or not: the trial ended in mating (mate y/n), the male attempted (attempt y/n), the female resisted (resist y/n), the male secured the female on his first attempt (first attempt successful y/n), and the male eventually secured the female within three attempts (success y/n) ( Figure 2). The dependent variable was whether the pair successfully moved to the next stage of the mating sequence or not. The independent variables were female size, male size, the interaction between female size and male size, and male pedipalpal femur length. To determine whether the morphological traits predicted the length of copulation and the length of postcopulatory contact, we used linear regressions with the same independent variables.
Finally, following Fowler-Finn et al. (2014) [18], we looked at whether the relationship between the male-female size difference and attempt length differed depending on whether the attempt was successful or not. For all but L. calcar (which had a normal distribution), we log-transformed attempt length as the dependent variable. Independent variables were whether or not the attempt was successful, the size difference between females and males (female size-male size), and the interaction term between the size difference and whether the attempt was successful.

Morphology
We found significant positive correlations between male body size and pedipalp length for L. vittatum and L. aldrichi, with marginally non-significant positive correlation in L. politum (Table 1). We found a range of sexual size dimorphisms across species, with L. calcar exhibiting the lowest dimorphism, and L. ventricosum exhibiting the largest dimorphism (Table 2). Table 1. Correlations between the principal component describing male body size and pedipalp length generated using Pearson product-moment correlations. Bolded values indicate p < 0.05, and 'n' indicates the number of individuals used in the analysis. Species are arranged from low antagonism at the top of the table to high antagonism at the bottom, and categorized by the possession of penile sacs (sacculate) or the absence of penile sacs (non-sacculate).

Species
Correlation

Predictors of Outcome
Predictors of overall outcome: In three of the five species studied, we identified morphological predictors of whether or not mating occurred. For these species-L. vittatum, L. calcar, and L. politum-female size was the primary predictor (Tables 3 and 4). For L. aldrichi, there was a marginally non-significant effect of female size on whether or not mating occurred. While larger females tended to mate more in L. vittatum, L. politum, and L. aldrichi, smaller females were more likely to mate in L. calcar (Tables 3 and 4). Table 3. Analyses testing for morphological predictors of various stages of mating in five species of leiobunum harvestmen. All of the models were either nominal logistic regressions or linear regressions. Bolded values indicated p < 0.05, and 'n' indicates the number of trials included in each analysis. The gray text in L. aldrichi indicates there was only one male that did not attempt. Sacculate species are on the left, and non-sacculate species are on the right, with the continuum of antagonism going from low antagonism to high antagonism, from left to right.

Sacculate Species
Non  Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right. t the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of ger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a e size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the e "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and tinuum of antagonism going from low antagonism to high antagonism from left to right.

Sacculate
Non-Sacculate L. aldrichi L. politum L. ventricosum L. calcar L. vittatum  Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right.   Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right.   Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right.   Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right.  Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right.  Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right.   Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right.   Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right.   s and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of ndicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a ge. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the ation of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and e right with the continuum of antagonism going from low antagonism to high antagonism from left to right.   Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right.   Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right.   Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right.   Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right.   Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right.   Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right.  Table 4. How the size of males and females predict the success and timing of each stage of mating in the five species of leiobunine harvestmen studied. The size of the male and female symbol indicate whether larger or smaller males/females were more likely to either be successful at a given stage, or more likely to have a longer duration of a given stage. When the relative size of males versus females predicted outcome, the '<' and '>' symbols indicate the relationship dictating the most successful or longest duration of a stage. The "-" indicates that neither male nor female body size predicted outcome. Sacculate species are on the left, and non-sacculate species are on the right with the continuum of antagonism going from low antagonism to high antagonism from left to right. Predictors of precopulatory interactions: There were no clear patterns of morphological predictors for whether a male attempted to secure the female. In L. calcar, males were more likely to attempt to mate with smaller females, and in L. aldrichi, males were more likely to attempt to mate with larger females (Tables 3 and 4). However, for L. aldrichi, these results are driven by a single male that did not attempt to mate with the smallest female in the experiment.

Sacculate
Whether or not the female resisted also had no clear patterns. In L. vittatum, resistance was more likely when interacting with males with shorter pedipalps; in L. ventricosum, resistance was more likely when the female was comparatively smaller than the male (Tables 3 and 4). We did find that female body size was a primary predictor of whether a male was successful in securing a female, either in his first attempt or by three attempts (Tables 3 and 4). Again, L. calcar showed the opposite pattern of the other species, with success being more likely with smaller females (Tables 3 and 4).
The relationship between attempt length and the male-female size difference did not depend on whether the attempt was successful for any of the species tested (whole model: p > 0.05 for all of the species).
Predictors of pericopulatory and postcopulatory outcomes: The outcomes of pericopulatory and postcopulatory interactions were driven primarily by either male size, or an interaction between male and female size (Tables 3 and 4). For the length of copulation: L. vittatum copulation lasted longer when the male was small, and in both L. vittatum and L. ventricosum, copulation was longer when the female was relatively larger than the male (Tables 3 and 4).
We found a mix of morphological predictors for the presence of guarding and the length of postcopulatory contact. In L. vittatum, males that were relatively smaller than the females were more likely to guard. In L. politum, larger males were more likely to guard, and also sustained postcopulatory contact for longer. Finally, in L. ventricosum, postcopulatory contact lasted longer when the female was larger.

Discussion
Male and female size predicted the outcomes and timing of various stages of mating in the five species of leiobunine harvestmen studied. However, these patterns varied across the mating stages as well as across species. We found that the success of precopulatory stages of mating was primarily predicted by female traits, whereas the success and length of copulatory and postcopulatory stages were primarily predicted by either male traits alone or the interaction between male and female traits. Despite this overall pattern, we found that the polarity of the relationship between size and outcome varied across species, and that this variation did not appear to correlate with the antagonism spectrum predicted by morphological characters in the clade [24].
The overall shift from female size predicting the outcome of early stages to male size predicting the outcome of later stages suggests some basic rules for mating interactions regardless of placement along the antagonism spectrum. The overall shift in predictors suggests that distinct sources of selection favoring different sets of traits may predominate during each stage of the mating process [18]. As male and female interests converge after they have assessed one another and approach fertilization [20], the sources of selection could shift [9,19], shaping different sets of traits [28]. For example, in earlier stages of mating interactions before males and females have had an opportunity to assess one another, tests of strength or size may dictate successful interactions, and later, more detailed assessments may occur [20], including an assessment of the traits favored by cryptic female choice such as nuptial gifts and sperm quantity [12]. Thus, it is not surprising that we observed a change in which traits predicted the outcome and timing of each stage of mating.
During precopulatory stages of mating, larger females were more likely to mate and be successfully secured (except in L. calcar). Larger females could be more likely to mate because of higher receptivity due to being in better condition [29] or to being more gravid (i.e., containing more mature eggs); alternatively, males could show a preference for larger females [30][31][32][33]. We suggest an increased receptivity due to gravidity because we found no correspondence between female size and of sacculate species contain a nearly 20% larger proportion of essential amino acids compared to non-sacculate species (Kahn et al.,in press [53]), and this could be a significant trait that females assess in making mating decisions.
We found significant differences in our dataset for L. vittatum compared with the 2014 study. Fowler-Finn et al. (2014) [18] found that successful attempts were shorter if females were larger, and unsuccessful attempts be longer when females are larger; this was interpreted as females making a decision and then resisting, which was a pattern we did not find in the current study. We also found other differences, notably including the higher success of males with shorter pedipalps in Fowler-Finn (2014). The current dataset encompasses a much wider range of collection dates, and we suggest the difference in results may reflect changes across a single mating season in mating dynamics, which we have found in multiple species in the clade (Fowler-Finn and Boyer, unpubl. data [54]). However, we also cannot fully rule out the potential for variation in experience in the field to shape some of the patterns that we describe for L. vittatum and the other species examined in the this study. The final interesting pattern that emerged from our study that is worth noting is that L. calcar differed from other species in that males were more successful across multiple stages of mating when females were smaller, and this species exhibited the lowest sexual dimorphism in weight among the species studied.

Conclusions
We found that size predicted mating interaction outcomes in varied ways across species and stages of mating in leiobunine harvestmen, suggesting a rapid and complex evolution of mating behavior and assessment in this diverse clade. Overall patterns progress from primarily female size being the primary predictorof success in earlier stages of mating, to male size and male size relative to female size predicting success and duration of later stages. However, the polarity of size and its influence on mating outcomes varies dramatically across species, suggesting different mechanisms dictating the dynamics of various mating stages in different species. We also find contrasting patterns in size predictors across stages of mating, which suggest the action of multiple sources of selection, and suggest that mating success does not necessarily equate fertilization success. This study paints a picture of a clade that is rich for studying the evolution of mating behavior and decisions, and future work tackling relevant mechanisms is likely to reveal interesting results.