Lingonberry Fruit Ethanol Extract Ameliorates DSS-Induced Ulcerative Colitis In Vivo and In Vitro

: Ulcerative colitis (UC) is an inﬂammatory chronic intestinal disease with pathological characteristics, including imbalanced immune function and the overexpression of inﬂammatory cytokines and mediators. Inﬂammatory cytokines (tumor necrosis factor (TNF)- α , interleukin (IL)-1, and IL-6) were oversecreted in UC condition. Cyclooxygenase (COX)-2 and prostaglandin (PG)E 2 were also overexpressed in colon tissue. Lingonberry (LB) ( Vaccinium vitis-idaea L.) possesses pharmacological activities, including anti-oxidant, anti-cancer, and anti-obesity effects. To explore LB’s effects on UC, BALB/c mice were administered with 3% (w/v) dextran sulphate sodium (DSS) and LB extract (70% ethanol) orally for nine days. The severity of UC was measured by the change in body weight and colon length. To evaluate LB’s regulatory effect on inﬂammatory cytokines, the enzyme-linked immunosorbent assay (ELISA) kit was used to measure the inﬂammatory cytokines in mouse serum. Mouse peritoneal microphages were used to detect LB’s anti-inﬂammatory effect. The results showed that LB treatment ameliorated less weight loss and longer colon length compared to the DSS-treated group. LB treatment also ameliorated the secretion of inﬂammatory cytokines. These results indicated that LB has potential as an herbal medicine to treat UC.


Introduction
Inflammatory intestinal diseases, including ulcerative colitis (UC) and Crohn's disease (CD), have a high re-occurrence ratio. These diseases cause chronic abnormal inflammation in intestinal tissues [1]. Dietary habits, genetics, and irregular lifestyles such as stress factors, alcoholism, or drug abuse increase a person's probability of developing UC [2].
UC is an increasing global health problem, yet little is known about its etiology. Currently, researchers recognize that UC is characterized by localized inflammatory symptoms in the submucosa or mucosa and inflammation in the large intestine [3]. Major clinical symptoms include diarrhea, abdominal pain, weight loss, and bloody stool causing inflammatory cell infiltration and colon length shortening. These symptoms can cause the life quality for UC patients to deteriorate [4]. If left untreated, UC can cause further complications, including colorectal cancer or peritonitis [5]. To prevent these secondary complications, treatment is necessary.
Despite there being little identification of the etiological causes of UC, various therapies such as 5-aminosalicylic acid (5-ASA) agents, immunosuppressive agents, and corticosteroids are available, but their side effects include nausea, fever, and a loss of appetite.
Long-term use of these therapies can result in kidney disorder, vascular disease, and hepatotoxicity [6]. The therapeutic agents stop diarrhea and reduce pain, but the relief is only temporary [7]. Finding therapeutic agents, especially ones that are of natural origin with long-lasting relief and no side effects, is crucial for UC patients.
Our UC mice model, induced with dextran sulphate sodium (DSS) (molecular weight: 36,000-50,000), exhibited UC symptoms including bloody stools, epithelial injury, ulceration, and inflammatory cell infiltration [8]. Crypt abscesses, epithelioglandular hyperplasia, and increased inflammatory cell infiltration were also observed [9]. These UC symptoms and colon conditions in the mouse model were similar to those of humans. This indicated that the UC mouse model-induced DSS was appropriate for studying the mechanism of therapeutic agents.
Lingonberry (LB) (Vaccinium vitis-idaea L.) contains pharmacological constituents, including anthocyanin, ellagitannin and phenolic acid [10]. LB is a reddish-colored berry that grows in Eurasia and North America. The phenolic compound in berries, such as LB, blueberry, cranberry and chokeberry, help treat oxidative disease [11]. LB is traditionally used in food not only for its red color, but also for its rich vitamins and polyphenols [12]. Previous studies have evaluated the pharmacological effects of LB on obesity [13], glucose metabolism [14], inflammation [15], oxidative stress [16], antibacterial activity [17], and cancer [18]. Moreover, the phenolic content of LB has anti-oxidant activity and antiinflammatory effects on RAW264.7 cells [19]. To date, however, the effect of LB on UC has not been studied.
Various studies suggest that an inflammatory response is a consequence of the interaction between immune-mediated cells (dendritic cells and macrophages) and antigens [20]. Macrophage activation induces UC via microbacterial factors such as lipopolysaccharides (LPS), and releases inflammatory-mediated factors containing interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α, and nitric oxide (NO). UC patients have been shown to have increased level of inflammation mediators [5]. Mouse macrophages have been used to investigate the effect of LB on UC.
In this study, we demonstrated LB's anti-inflammatory activities in a UC mouse model by DSS administration.

Preparation of LB Extract
The powder of dried LB fruit was purchased from HAENA Food (Seoul, Korea). The powder of LB was extracted with 70% ethanol under reflux at 70 • C for 3 h (50 g/L). The ethanol solvent was filtered with filter paper (ADVANTEC, Tokyo, Japan). The filtered solvent was evaporated using a rotary evaporator at 45 • C. Then the solvent was freezedried at −45 • C, 5mTorr. The yield of the dried extract was 11.26%. The LB extract was diluted in distilled water and filtered through a 0.22 µm syringe filter (HYUNDAI Micro, Seoul, Korea). A voucher specimen (JUHES-1665) was deposited at the Department of Oriental Medicine Resources, Jeonbuk National University (Iksan, Korea).

Animals
BALB/c mice (male, 6 weeks, 22 ± 1 g) and C57BL/6 mice (male, 6 weeks, 20 ± 1 g) were supplied by SAMTACO (Osan, Korea). All mice were provided with tap water and group housing under standard conditions (20-24 • C; 40-60% humidity) throughout the study. The mice were acclimated for seven days and divided randomly into five experimental groups, each containing six mice per cage. The mice were sacrificed on day 9, and their colons were collected. After the length of the colons was measured, the colons were cut into small pieces (approximately 2 to 3 cm) and kept in 15 mL tubes. The tips of the colons (approximately 8 mm) were fixed in formalin for histological analysis. The experimental schedule and procedure (2017-0086) were allowed by the Committee on the Care of Laboratory Animal Resources, Jeonbuk National University.

Induction of Colitis
To induce UC, the drinking water was contaminated with 3% (w/vol) DSS for 9 days naturally in the male BALB/c mice. The mice were bred into five randomly divided groups (n = 6): a Blank group (distilled water orally once per a day), DSS group (3% DSS with distilled water orally once per a day), LB 10 group (3% DSS with 10 mg/kg LB orally once per a day), LB 100 group (3% DSS with 100 mg/kg LB orally once a day), and 5-ASA group (3% DSS with 50 mg/kg 5-ASA orally once per a day). Gross bleeding in the stool or at the anus, stool condition, and bodyweight were recorded daily to estimate UC activity index. At the end of each experimental day, a blood sample was drawn from the orbital vein under diethyl ether anesthesia.

Disease Activity Index (DAI)
To assess the severity of UC, the DAI score was used in this experiment. Body weight, gross bleeding, and stool condition were recorded daily ( Table 1). The DAI score was evaluated as the score average for the criteria mentioned. DAI = {(weight loss) + (diarrhea score) + (rectal bleeding score)}/3 Mouse serum samples were prepared and subjected at the end of each experimental day to measurement of serum TNF-α, IL-1β, and IL-6 using the ELISA kit. In brief, 100 µL of capture antibodies were added to each well of the 96-well plates and then incubated at 4 • C overnight. After each well was washed with 0.05% phosphate buffered saline containing Tween 20 (PBST), 100 µL of each serum sample was added into each well and incubated at room temperature (RT) for 3 h. The 96-well plates were washed three times, then 100 µL of detection antibodies were added to each well and incubated at RT for 1 h. Next, each well was coated with 50 µL of avidin-HRP for 30 min. A substrate mixture 100 µL was added and reacted into each well, and the plates were incubated at RT for 35 min in a light-blocked place. Absorbance was determined with a VersaMax microplate reader (Molecular Devices, Sunnyvale, CA, USA) at 450 nm wavelength.

Western Blotting Analysis
The mice were sacrificed on day 9 and the colons were dissected (8-10 mm) and flushed with PBS to perform Western blot analysis. The colon segments from each group were homogenized with PRO-PREP lysis buffer (iNtRON Biotech, Seongnam, Korea) for 1 h on ice. The lysates were centrifuged at 15,000 g for 15 min at 4 • C. The supernatants were moved to a 1.5 mL distilled tube. Concentrations of protein were quantified by a detergent compatible (DC) protein assay kit (Bio-Rad Laboratories, Inc., Hercules, CA, USA) according to the producer's protocol. The protein extract samples (30 µg protein) were separated by 10% sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. The separated protein was transferred to polyvinylidenedifluoride (PVDF) membrane (Merck, Temecula, CA, USA). The membranes were blocked with 5% non-fat milk in 0.05% PBS-Tween 20 (PBST) for 1 h at RT. Next, the primary antibodies of iNOS and GAPDH (dilution, 1:1000) were incubated with the membranes overnight at 4 • C. The membranes were washed three times with 0.05% PBST then incubated with horseradish peroxide (HRP)-conjugated anti-rabbit and anti-mouse 2 nd antibodies (dilution, 1:10,000) for 1 h at RT. Antibody-specific proteins were identified using Western blot luminol reagent (Santa Cruz Biotechnology, Dallas, TX, USA) and visualized with the LAS-4000 image reader (Fujifilm Life Science, Tokyo, Japan). The concentrations of the protein bands were quantified using the Image J software (NIH).

Hematoxylin and Eosin Staining (H&E Staining)
The entire colon of each mouse was dissected and washed with cold PBS. About 5-8 mm of rectum sections were fixed in 10% neutral buffered formalin (Sigma-Aldrich) for 24 h at RT. Then sections were embedded in paraffin (0.4 µm) to provide slices for histological estimation. These colon slices were stained with hematoxylin and eosin. The score of histological injury was determined based on the previously described criteria [21] (Table 2). Loss of one-third of the crypts 2 Loss of two-thirds of the crypts 3 Lamina propria covered with single layer of epithelial cells with mild inflammation cell infiltration 4 Erosions and marked inflammatory cell infiltration 2.9. Immunohistochemistry (IHC) IHC was carried out as described previously [22]. All paraffin sections were deparaffinized using xylene and rehydrated using ethyl alcohol and distilled water. The retrieval of antigens was performed in citrate buffer (pH 6.0, 10 mM citrate buffer with Tween 20) for 10 min in the microwave. Experimental slides were blocked with 3% H 2 O 2 for 10 min for endogenous peroxidase activity and pre-blocked with a normal blocking serum for 10 min. Then the slices were incubated with a primary antibody in block buffer (1:50) at 4 • C overnight. After that, all slides were washed three times with tris-buffered saline (TBS) with 0.1% Tween-20 (TBST), and the following processes were performed with the Universal Quick kit (Vectastain, Vector Laboratories, Inc., Burlingame, CA, USA) according to the producer's procedure. All the slides were stained with peroxide substrate solution (Vectastain) and counter-stained with hematoxylin for 10 min. All experimental slides were secured with a mounting solution and cover slips. Experimental images were observed through optical microscopy (Primo Star, Zeiss, Berlin, Germany).

Prostaglandin (PG)E 2 Assay
The mouse colons were washed with cold PBS and stored at −75 • C immediately until the experiment. The colon tissues were homogenized with 1 mL ice-cold lysis solution (10 mM Tris-HCI (pH 7.4), 1% protease and phosphatase inhibitor cocktail, and 1 mM EDTA, 10 µM indomethacin (Sigma, Saint Louis, MO, USA)). The tissue homogenates were moved to microcentrifuge tubes and centrifuged at 4 • C at 13,000× g for 10 min. The protein concentrations in the supernatants (5 µL) were analyzed by a DC Protein Assay Kit (Bio-rad) according to the producer's procedure. The measurement of PGE 2 levels was carried out using a PGE 2 ELISA Kit (Enzo bioscience, Farmingdale, NY, USA). All samples were diluted in assay buffer in a ratio of 1:100. The experiments were performed in triplicates and a standard curve was created at the same time. Absorbance was measured using the Versamax microplate reader at 405 nm.

Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR)
The primer sequences are shown in Table 3. Briefly, the total RNA of cells was extracted with the easy-BLUE total RNA extraction kit (iNtRON) according to the manufacturer's protocol. The extracted total RNA was reverse transcribed into cDNA using the AMPIGENE ® cDNA Synthesis Kit (Enzo Bioscience, Farmingdale, NY, USA) and qRT-PCR amplification was conducted with the SYBR Green reagent to measure the relative mRNA expressions of IL-1β, TNF-α, and IL-6.

Determination of Myeloperoxidase (MPO) Activity
Myeloperoxidase (MPO) activities were measured, and, in the process, neutrophil infiltration of the colon was assessed indirectly. Mid-colon sections were homogenized with EDTA/NaCl buffer (pH 4.7) and then centrifuged at 12,000× g for 15 min. The homogenized colons were re-suspended in 0.5% hexadecyltrimethyl ammonium bromide buffer (pH 5.4). The colon samples were deep-frozen in liquid nitrogen and defrosted three times repeatedly. Samples were centrifuged again and then the 25 µL supernatant was used for the MPO activity measurement. The enzyme reaction was performed with the addition of 25 µL of NaPO 4 , 1.6 mM tetramethylbenzidine (TMB) in 80 mM, and 0.3 mM H 2 O 2 100 µL. The MPO activity of each sample was measured with a Versamax microplate reader at 560 nm wavelength and the results were represented as density per milligram of colon tissue.

Isolation and Culture of Peritoneal Macrophages
C57BL/6 male mice were administered with an intraperitoneal (IP) injection of 4% thioglycolate (TG) solution (3 mL) and sacrificed on the third day after injection. Peritoneal cavities were washed with Dulbecco's modified Eagle's medium (DMEM) (5 mL). The peritoneal lavage fluid was centrifuged at 6000× g for 5 min. The cells were re-suspended in DMEM and seeded into 24-well microplates. These cells were cultured at 37 • C for 24 or 48 h in DMEM plus 10% fetal bovine serum (FBS).

Cell Viability Assay
To investigate cell viability, thiazolyl blue tetrazolium bromide (MTT) was used. Mice peritoneal macrophages were pre-treated with LB (1, 10, 100, or 1000 µg/mL) for 24 h. MTT solution (0.5 mg/mL) was then added and incubated over 4 h. After incubation, the supernatant was eliminated, and the formazan crystals were liquefied in 650 µL of dimethyl sulfoxide (DMSO). The absorbance of each well at 540 nm wavelength was determined with the Versamax microplate reader.

Measurement of Nitric Oxide Level
Mouse peritoneal macrophages were pre-treated with LB (1-1000 µg/mL) for 4 h. Next, they were treated with 1 µg/mL of LPS and incubated for 48 h. After incubation, the supernatants were collected for NO determination. To measure the nitrite oxide level, a same volume of Griess reagent A and B (1% sulfanilamide in distilled water/0.1% naphtylethyenediamine dihydrochloride in 5% H 3 PO 4 ) was mixed with the supernatant at RT for 10 min. The Versamax microplate reader measured the absorbance at a wavelength of 540 nm to determine nitrite concentration. NaNO 2 was used to obtain the standard curve.

Statistical Analysis
The Graph Pad Prism software 5.0 analyzed the results, which were presented as the mean ± S.D. (standard deviation) of at least three experiments separately. Statistical evaluation was identified by using a Bonferroni's post-hoc analysis and one-way analysis of variance (ANOVA). A p-value of <0.05 was considered a statistically significant difference.

Effect of LB on Clinical Signs in DSS-Induced UC Mice
BALB/c mice were orally administered 3% DSS and LB 10 mg/kg, LB 100 mg/kg, and 5-ASA 50 mg/kg daily for nine days to regulate UC condition. 5-ASA was used as the positive control. The DSS group had significant body weight loss compared to the Blank group. In both LB groups, loss of body weight was attenuated compared to the DSS group (Figure 1a) (Blank group, 23.5 ± 0.80 g; DSS group, 20.3 ± 1.03 g; LB 10 group, 23.0 ± 0.26 g; LB 100 group, 22.6 ± 0.95 g; 5-ASA group, 22.0 ± 0.92 g). The DAI score was measured during the experimental period. The DAI score was significantly elevated in the DSS group, but the administration of LB 100 mg/kg decreased the DAI score (Figure 1b). LB 100 mg/kg was more effective than LB 10 mg/kg on the suppression of the DAI score (3% DSS group, 3.7 ± 0.5; LB 100 mg/kg group, 2.3 ± 0.5; LB 10 mg/kg group, 3.2 ± 0.8; 5-ASA group, 2.3 ± 0.6).

LB Administration Recovered Colon Length Shortening
Colon length was assessed at the end of the experiment to evaluate the regulatory effect of LB. The average colon length was shorter in the DSS group compared to the Blank group (Blank group, 8.47 ± 0.22 cm; DSS group, 5.42 ± 0.11 cm). The colon length shortening slowed and improved in the LB group (DSS group, 5.42 ± 0.11 cm; LB 10 mg/kg group, 6.57 ± 0.42 cm; LB 100 mg/kg group, 7.02 ± 0.62 cm; 5-ASA group, 7.43 ± 0.10 cm) (Figure 1c).

LB Decreases the Secretion of Inflammatory Cytokines in DSS-Induced UC Mouse Serum
To determine LB's influence on inflammatory cytokine release, serum was gathered at the end of the experiment. ELISA calculated the secretion levels of inflammatory cytokines (TNF-α, IL-1β and IL-6). In the serum, the secretion of cytokines was significantly increased in the DSS group compared to the Blank group. In the LB 100 group, the secretion of cytokines was noticeably lower than in the DSS group (Figure 3). In addition, PGE 2 expression had the highest concentration in the DSS group (Blank group, 253.39 ± 13.08 pg/mL; DSS group, 1589.92 ± 87.60 pg/mL). The LB treatment, however, considerably suppressed PGE 2 expression in the colon tissue (DSS group, 1589.92 ± 87.60 pg/mL; LB 10 group, 695.79 ± 145.75 pg/mL; LB 100 group, 397.24 ± 61.54 pg/mL) (Figure 3d). These results indicate that LB (100 mg/kg) was similar to 50 mg/kg of 5-ASA, which was used as the positive control.

LB Attenuates the Expression of Cyclooxigenase-2(COX-2), NF-κB, and iNOS in DSS-Induced UC Mice
IHC staining was carried out to measure the expression of COX-2, NF-κB, and iNOS in the mice's colonic tissue. NF-κB expression in the DSS group was highest among the experimental group, but NF-κB expression was significantly lower in the LB 100 group than in the DSS group (Figure 4). The expression of COX-2 and iNOS in the DSS group was higher than in the Blank group. The iNOS and COX-2 expressions were, however, remarkably low in the LB 100 group.

LB Regulates the mRNA Expression of Inflammatory Cytokines in LPS-Stimulated Peritoneal Mouse Macrophages
To evaluate LB's anti-inflammatory effect, a peritoneal macrophage was extracted from C57BL/6 mice. The peritoneal mouse macrophages were pre-treated with various concentrations of LB (0.1 to 1000 µg/mL) for 24 h, then cell viability was estimated with the MTT assay. The LB did not show cytotoxicity in the peritoneal mouse macrophages (Figure 5a) that were stimulated with 1 µg/mL of LPS to secrete inflammatory cytokines. The IL-1β mRNA level increased with LPS stimulation only in the LPS-treated group and not the non-LPS group. The LB treatments (10, 100, and 1000 µg/mL) reduced the mRNA levels of IL-1β and IL-6 ( Figure 5b,c). LPS (1 µg/mL) stimulation increased the mRNA expression of TNF-α in peritoneal mouse macrophages, but the LB treatment decreased the TNF-α mRNA level (Figure 5d), as well as the IFN-γ mRNA (Figure 5e). These results show that LB effectively regulated LPS-stimulated inflammation in peritoneal mouse macrophages.

LB Regulates the NO Production in LPS-Stimulated Peritoneal Mouse Macrophages
NO production increased when peritoneal mouse macrophages were stimulated with 1 µg/mL of LPS compared to the non-treatment group (non-treatment group, 54.99 ± 1.05 µM; 1 µg/mL LPS treatment group, 252.04 ± 2.51 µM). LB treatment decreased NO production, especially 1000 µg/mL of LB, which significantly reduced it (1 µg/mL LPS treatment group, 252.04 ± 2.51 µM; 1000 µg/mL LB treatment, 172.89 ± 2.34 µM) (Figure 6a). The LB treatment regulated the mRNA level of iNOS in LPS-stimulated peritoneal mouse macrophages, and attenuated the protein expression of iNOS (Figure 6c). This result suggests that LB has an anti-inflammatory effect on macrophages.

Discussion
UC is a relapsing inflammatory chronic intestinal malady [23] that is worsened by an imbalanced inflammatory response and oxidative stress [24]. In addition, genetic factors, changes in environment, and an imbalanced immunological function can lead to difficulty in treating the intestinal inflammatory disease [25]. However, various drug treatments, including corticosteroids, immune modulators, and sulfasalazine, have serious side effects, such as poverty of blood, vomiting, and abdominal pain [26]. Therefore, the development of natural original products with few side effects is needed to treat chronic intestinal inflammatory disease.
Compared to various UC in vivo models, the DSS-induced UC model is appropriate as it mimics the characteristics of the disorder. Our DSS-induced mouse model exhibited UC symptoms including bloody stool, bodyweight loss, and diarrhea [27]. In this study, the administration of DSS worsened the clinical sign in vivo, but the LB treatment regulated the clinical sign of the DSS-induced mice. The administration of LB helped slow the bodyweight loss and decrease the DAI (diarrhea, occult/gross bleeding). Additionally, LB significantly slowed or stopped the colon length shortening caused by DSS (Figure 1). These results indicated that LB attenuated DSS-induced UC symptoms.
In inflammatory intestinal conditions, COX-2 leads to production of PG [28]. PGE 2 is over-expressed in UC colon tissue. Both PGE 2 and COX-2 increase in the mucosa [29]. 5-ASA used in UC treatment inhibits COX-2 activation [30]. In our experiment, 5-ASA was also used as the positive control. However, LB 100 mg/kg showed more significant effects on the regulation of PGE 2 ( Figure 3) and COX-2 ( Figure 4) activities than 5-ASA. These results suggest that LB extract can be a therapeutic agent for regulating intestinal inflammation. The transcription factor NF-κB modulates COX-2 synthesis and inflammatory cytokine secretion [31]. In IHC assay, intestinal NF-κB and iNOS expressions were increased by DSS drinking. However, those were particularly decreased due to LB 100 mg/kg administration. These experimental results suggest that the anti-UC effect of LB might be via COX-2 and NF-κB pathway.
MPO, an enzyme found in neutrophil, is an indicator of neutrophil infiltration and the severity of inflammation [32]. LDH, a ubiquitous enzyme, is released into the injured tissue or bloodstream [33]. The results showed that 100 mg/kg LB regulated MPO and LDH activity in DSS-induced colon tissue injury (Figure 2). The effect of LB administration on MPO and LDH activity also indicates the efficacy of ameliorating acute intestinal inflammation. Under DSS-induced UC condition, crypt damage and inflammatory cell infiltration occurred in inflamed areas and were relieved by LB administration (Figure 2). These results indicate that LB treatment suppressed DSS-induced colon damage.
Neutrophils, macrophages, and T cells release inflammatory cytokines (IL-1β, IL-6, IFN-γ, and TNF-α) that cause colon inflammation [34,35]. NO is also recognized as a regulator or mediator that induces inflammation [36]. Regulating inflammatory cytokines and NO is the strategy to remedy UC. In this study, LB suppressed the secretion of IL-1β, IL-6, TNF-α, and IFN-γ ( Figure 5) and NO production ( Figure 6) in LPS-stimulated peritoneal mouse macrophages. The interaction between the intestinal immune system and inflammatory cytokines could cause intestinal homeostasis and a disruption of tight junction factors [37]. Increased serum inflammatory cytokines (IL-1β, IL-6, and TNFα) by DSS administration were suppressed by LB administration, especially 100 mg/kg of LB extract. The anti-inflammatory and anti-UC efficacy were demonstrated through inflammatory cytokine inhibition in vivo and in vitro.

Conclusions
In conclusion, the LB treatment ameliorated the development and progression of UC in vivo and in vitro. LB, especially 100 mg/kg, regulated bodyweight loss, colon length shortening, and DAI in the DSS-induced UC mouse model. It also suppressed MPO activity and COX-2 and PGE 2 expression in colon tissues, as well as reduced inflammatory cytokines (IL-1β, IL-6, TNF-α, and IFN-γ) and NO production in LPS-stimulated peritoneal mouse macrophages. LB treatment also suppressed the histological injury and inflammatory mediators (NF-κB, iNOS) caused by DSS. This study suggests that LB is a potential therapeutic agent for UC.  Data Availability Statement: Datasets used and/or analyzed in the current study are available from the corresponding author on reasonable request.

Conflicts of Interest:
The authors declare no conflict of interest.