Gastro-Protective and Anti-Oxidant Potential of Althaea officinalis and Solanum nigrum on Pyloric Ligation/Indomethacin-Induced Ulceration in Rats

Recently, an alternative disease treatment approach is the research of medicaments from traditional medicine. Plants with anti-oxidant capabilities are used as herbal treatments for ulcer diseases. Medicinal/herbal extracts containing phytoconstituents have significant anti-ulcer activities in in vivo experiments on animal models, compared to reference drugs. The current study aims to inspect gastro-protective as well as in vitro and in vivo anti-oxidant potential of Althaea officinalis and Solanum nigrum extracts on pyloric-ligation/indomethacin-induced gastric-ulceration in rats. Rats were divided into six groups: normal control, gastric ulcer control, two standard pretreatment groups receiving omeprazole and misoprostol, and two test pretreatment groups receiving Althaea officinalis and Solanum nigrum. Pretreatments were administrated orally for 14 days. On the 15th day, animals, excluding the normal control group, were exposed to pyloric-ligation followed by indomethacin injection. After four hours, the rat’s stomachs were removed and gastric juice and blood samples were collected. Pyloric-ligation/indomethacin administration caused considerable elevation in ulcer number, ulcer index, acid and pepsin productivity, aggressive factors, and gastric mucosal lipid-peroxide contents. Moreover, reduction in titratable acidity, gastric mucosal nitric-oxide, anti-oxidant contents, and protective factors accompanied gastric-ulceration. Additionally, elevation in pro-inflammatory cytokines content and reduction in cystathionine-β-synthase and heme-oxygenase-1 expression was witnessed. Omeprazole, misoprostol, Althaea officinalis, and Solanum nigrum pretreatments fixed blood and tissue biomarkers, thereby protecting them from pyloric-ligation/indomethacin-induced gastric-ulceration in rats, which is hopeful for clinical examinations.


Introduction
Today, gastric hyperacidity and gastroduodenal ulcers represent a serious global problem [1]. A gastric ulcer is generated by an imbalance between the defense system and a damaging force in the All used chemicals, solvents, and reagents were purchased and obtained from authorized sources and of analytical grade. Omeprazole was purchased as Omepak ® 40 mg capsules (South Egypt Drug Industries Company "SEDICO", 6th of October City, Egypt). Misoprostol was obtained as a gift from Sigma Pharmaceutical Company, Egypt. Assay: 1.04%. Gastrin (catalog number: MBS704511), histamine (catalog number: MBS732202), SOD (catalog number: MBS036924), PG-E 2 (catalog number: MBS705028), and PG-I 2 (catalog number: MBS743424) reagent kits were purchased as the rat ELISA kit from MyBiosource, Inc., Southern California, San Diego, CA, USA. The MDA reagent kit was purchased as the rat ELISA kit (catalog number: LS-F28018) from Life Span BioSciences, Inc., Seattle, Washington, DC, USA. The NO reagent kit (catalog number: 917-010) was purchased as the nitric oxide (NO 2 − /NO 3 − ) assay kit from Assay Designs TM , Inc., Ann Arbor City, MI, USA. The GSH reagent kit was purchased as the rat ELISA kit (catalog number: E02G0367) from ShangHai BlueGene Biotech co., LTD., Shanghai, China. The primary antibodies for western blot analysis of TNF-α, IL-1β, CBS, and HO-1 were purchased from Thermo-Fisher Scientific, Rockford, IL, USA.

Overview of Methodology
To fulfill the purpose of the present investigation, a preliminary phytochemical study of the prepared extracts (aqueous extract of Althaea officinalis and ethanolic extract of Solanum nigrum) used in this study had to be done followed by evaluation of the in vitro anti-oxidant activity of the extracts (DPPH assay). After the dosing period and collection of tissue, blood, and gastric juice samples, macroscopic examination (ulcer number, ulcer index, and preventive index) and acid productivity assessment (gastric volume, titratable acidity, and acid output) were done. This was accompanied by assessment of aggressive factors like peptic activity, histamine and gastrin contents, and measurement of oxidative stress biomarkers including superoxide-dismutase (SOD) activity and reduced glutathione (GSH), nitric oxide (NO), and malondialdehyde (MDA) contents and protective factors including prostaglandin-E 2 (PG-E 2 ) and prostacyclin (PG-I 2 ) contents in accordance with assessment of immunological factors including tumor-necrosis-factor-alpha (TNF-α), interleukin-1-beta (IL-1β), cystathionine-β-synthase (CBS), and heme-oxygenase-1 (HO-1) expressions. All these parameters were assessed via enzyme-linked immunosorbent assay (ELISA) and western blot analysis. Microscopic histopathological examinations were also performed using routine hematoxylin and eosin (H and E) staining and special alcian blue staining for determination of gastric wall mucus (mucin content).

Preliminary Phytochemical Screening of Extracts
The extracts of Althaea officinalis and Solanum nigrum were subjected to preliminary phytochemical screening [38,39]. These extracts were qualitatively analyzed for the presence or absence of phytochemical constituents employing standard analysis methods. These methods are conducted to detect the presence or absence of carbohydrates by the Molisch test, tannins by the FeCl 3 test, flavonoids by the NaOH/HCl test, saponins by the froth test, and for glycosides: anthraquinon glycosides by the Born-trager test, cardiac glycosides by the Keller-Killiani test and cyanogenic glycosides by the sodium picrate paper test, sterols and triterpenoids by the Salkowski test, alkaloids by the Dragendorff test, and volatile oils by the Sudan III test and by checking if the extract had a characteristic odor or not.

Evaluation of the In vitro Anti-Oxidant Activity (DPPH Assay)
The in vitro anti-oxidant potential of test extracts was determined according to the method described by Takao et al. which was modified by Delazar et al. [40,41]. Briefly, a solution of 4 mg DPPH in 50 mL methanol was prepared to get a final concentration of 80 µg/mL. Each extract was diluted in methanol to get different dilutions ranging from 20-1000 µg/mL. A mixture of 1 ml of each diluted extract with DPPH (1 mL) was prepared and stood at room temperature for 30 min and acted as test samples. A mixture of 1 ml of methanol and DPPH (1 mL) was prepared and acted as a control sample. The optical density of all mixtures was determined at 517 nm. Positive control samples using ascorbic acid were prepared by the same method. The DPPH radical scavenging activity of test and positive control samples were calculated according to the following equation: where: A 0 is the optical density of the control sample and A 1 is the optical density of the test or positive control sample after 30 min. The IC 50 value was estimated as the concentration (µg/mL) of the test or positive control samples required to scavenge 50% of DPPH.

Induction of Gastric Ulcer and Sample Preparation
On the 15th-day of pretreatment or vehicle administration and after 36 hours of starvation, rats were anesthetized with ether inhalation, carefully dissected, and exposed to pyloric-ligation [43] for gastric juice collection. After that, indomethacin (25 mg/kg) [44,45] was injected intra-peritoneally, excluding the normal control group which received a single intra-peritoneal injection of 1% saline solution with Tween 80 (vehicle of indomethacin). After 4 h, rats were killed by cervical dislocation under ether anesthesia. Stomachs from all rats in each group were removed and the gastric juice collected. Blood samples were collected from the retino-orbital sinus. The stomach mucosal tissue was then opened and assessed for macroscopic examination. Acid productivity parameters were determined and gastric mucosal homogenates were prepared in normal saline.

Macroscopic Examination (Assessment of Gross Mucosal Damage)
The glandular part of the stomach was inspected and examined for macroscopical mucosal lesions. The number of lesions was counted using an illuminated magnifying lens (10×) [46,47]. The ulcer index (mm) for each group was determined by the earlier method described by Cho and Olge [48] indicating that the ulcer index is the mean of total ulcer length and hemorrhagic spots in the rats of each group. Hano et al. [49] determined the equation of preventive index calculation as follows: Preventive Index (%) = Ulcer Index (Ulcer Control group) − Ulcer Index (Treated group) Ulcer Index (Ulcer Control group) × 100 (2)

Microscopic Histopathological Examination (Routine H and E Staining and Alcian Blue Staining)
All data and micrographs of microscopic examinations were obtained with the aid of a full HD microscopic camera operated by the Leica application module for tissue section analysis (Leica Biosystems, Wetzlar, Germany).

Routine H and E Staining (Histopathological Examination of Stomach)
The method of routine hematoxylin and eosin (H and E) staining was described by Drury and Wallington for estimating histological assessment of the structure of the stomach tissue [50]. The ulcers, necrotic changes, edema, and inflammatory cell infiltration in the gastric mucosa were determined in all experimental groups.

Alcian Blue Staining for Determination of Gastric Wall Mucus (Mucin Content)
The method of the determination of gastric wall mucus was described by Corne et al. [51] using alcian blue (pH = 2.5) for acidic mucosubstances staining. This method was done with some modifications as it is reported that alcian blue can bind to mucus cells in gastric mucosa, indicating the amount of mucus [52]. The % area of alcian blue positive goblet cells and mucin in gastric mucosa were quantified in 5 random non-overlapping fields per tissue section in a blind manner with total sections (number = 5 per group).
2.11. Acid Productivity Parameters (Gastric Volume, Titratable Acidity, and Acid Output Determination) After centrifugation of the collected gastric juice at 3000 rpm for 10 min, and elimination of solid mass, the volume of gastric juice (mL) was measured. When the volume of solid mass was more than 0.6 mL, the sample was rejected [43]. A portion of 0.2 mL of gastric juice was titrated with 0.01 N sodium hydroxide using phenolphthalein as an indicator to determine titratable acidity as described by Shay et al. [53] and Grossman [54]. After that, acid output was determined according to the method described by Brodie and Hooke [55] as microequivalents per 4 h.

Peptic Activity Determination
Pepsin activity is the major cause of the proteolytic activity of gastric secretion. In brief, this proteolytic activity was measured by the amount of proteases produced after 30 min of incubation of casein substrate with pepsin. This activity of pepsin was measured in gastric juice spectrophotometrically at 280 nm [56].

Biomarkers Estimated Using ELISA Technique
Plasma gastrin as well as gastric mucosal histamine content, oxidative stress biomarkers (gastric mucosal GSH and MDA contents and SOD activity), and protective factors (gastric mucosal PG-E 2 and PG-I 2 contents) were estimated using ELISA test reagent kits in compliance with the manufacturer's instructions at 450 nm.
Assessment of total NO content in gastric mucosal homogenate was estimated using a nitric oxide (NO 2− /NO 3− ) assay kit in compliance with the method described by Kendrich et al. [57] at 540 ± 20 nm.
These biomarkers were measured by the aid of an ELISA Processing System (model: Spectra Max Plus-384 Absorbance Microplate Reader, San Jose, CA, USA).

Immunological Markers (Gastric Mucosal TNF-α and IL-1β Contents and CBS and HO-1 Activities) Estimated by Western Blot Analysis
Tissue levels of TNF-α, IL-1β, CBS, and HO-1 were measured using the western blot technique described earlier [58,59] via a BioRad mini protein electrophoresis separation unit (model: 1658004, Sinorica international patent and trademark, Germantown, MD, USA). Briefly, a portion of the stomach glandular tissue was subjected to RIPA lysis buffer, and then the lysed samples were brought to complete protein extraction. The lysate was kept in ice for 30 min on a shaker and cell debris was removed by centrifugation at 16,000× g using a cooling centrifuge. Supernatants were transferred to new tubes for protein concentration determination. Protein separation by gel electrophoresis was performed using the procedure termed SDS-PAGE (sodium dodecylsulfate-polyacrylamide gel electrophoresis) via the proper antibodies. Briefly, incubation was done overnight in each primary antibody solution, against the blotted target protein, at 4 • C. The blot was rinsed 3-5 times for 5 min with TBST (Tris-buffered saline with Tween 20). Incubation was done in the horseradish peroxidase (HRP)-conjugated secondary antibody solution against the blotted target protein for 1 h at room temperature. The blot was rinsed 3-5 times for 5 min with TBST. The chemiluminescent substrate was applied to the blot according to the manufacturer's recommendation. The chemiluminescent signals were captured using a CCD camera-based imager. Image analysis software was used to read the band intensity of the target proteins against the normal control sample after normalization by beta-actin on the Chemi-Doc MP Imager.

Statistical Analysis
Statistical analysis and the significance of difference between groups' means were performed via one-way ANOVA, and after that a Tukey-Kramer multiple comparisons test was done, by the aid of GraphPad Instat computer software (GraphPad Software, San Diego, CA, USA). In addition, Microsoft Excel 2010 software (Microsoft Corporation, Microsoft Redmond campus in Redmond, WA, USA) was used to create graphs and tables.

Preliminary Phytochemical Screening of Extracts
Preliminary phytochemical analysis for Althaea officinalis extract was positive for carbohydrates, traces of tannins, flavonoids, traces of saponins, and volatile oils. Preliminary phytochemical analysis for Solanum nigrum extract was positive for carbohydrates, tannins, flavonoids, saponins, sterols and triterpenoids and alkaloids ( Figure 1 and Table 1).

Evaluation of the In Vitro Anti-Oxidant Activity (DPPH Assay)
Aqueous extracts of Althaea officinalis and ethanolic extracts of Solanum nigrum showed free radical scavenging activities against DPPH. The ethanolic extract of Solanum nigrum was the more active as it shows the smallest IC50 values. The aqueous extract of Althaea officinalis was the least active as it shows the highest IC50 values. The ethanolic extract of Solanum nigrum (IC50 = 295.84 ± 0.11 µg/mL) showed a significant difference when compared to the standard ascorbic acid (IC50 = 10 ± 0.03 µg/mL). The aqueous extract of Althaea officinalis (IC50 = 443.60 ± 0.39 µg/mL) showed a significant difference when compared to the standard ascorbic acid and ethanolic extract of Solanum nigrum ( Figure 2).

Evaluation of the In Vitro Anti-Oxidant Activity (DPPH Assay)
Aqueous extracts of Althaea officinalis and ethanolic extracts of Solanum nigrum showed free radical scavenging activities against DPPH. The ethanolic extract of Solanum nigrum was the more active as it shows the smallest IC 50 values. The aqueous extract of Althaea officinalis was the least active as it shows the highest IC 50 values. The ethanolic extract of Solanum nigrum (IC 50 = 295.84 ± 0.11 µg/mL) showed a significant difference when compared to the standard ascorbic acid (IC 50 = 10 ± 0.03 µg/mL). The aqueous extract of Althaea officinalis (IC 50 = 443.60 ± 0.39 µg/mL) showed a significant difference when compared to the standard ascorbic acid and ethanolic extract of Solanum nigrum ( Figure 2).

Macroscopic Examination (Assessment of Gross Mucosal Damage) (Ulcer Number, Ulcer Index, and Preventive Index)
Exposing rats to pyloric-ligation/indomethacin administration (gastric ulcer control group) resulted in significant ulceration in the glandular part of their stomachs as it expressed elevations in ulcer number and ulcer index of 13.57 ± 2.89 and 29.71 ± 7.85, respectively, in comparison to the normal control group. Omeprazole pretreatment expressed a significant reduction in ulcer number and ulcer index, to 22.11% and 35.09%, respectively, in comparison to the gastric ulcer control group, providing 64.90% protection against gastric mucosal injury. Similarly, misoprostol pretreatment expressed a significant reduction in ulcer number and ulcer index, to 29.48% and 42.79%, respectively, in comparison to the gastric ulcer control group, providing 57.21% protection. In addition, Althaea officinalis pretreatment expressed a significant reduction in ulcer number and ulcer index, to 44.21% and 44.23%, respectively, in comparison to the gastric ulcer control group, providing 55.77% protection. Solanum nigrum pretreatment expressed a significant reduction in ulcer number and ulcer index, to about 37.89% and 44.71%, respectively, in comparison to the gastric ulcer control group, providing 55.29% protection ( Figure 3). Statistical analysis was performed via one-way ANOVA test, and after that the Tukey-Kramer multiple comparisons test was done. Where: * indicates a significant difference from ascorbic acid (p < 0.05), and @ indicates a significant difference from Althaea officinalis (p < 0.05).

Macroscopic Examination (Assessment of Gross Mucosal Damage) (Ulcer Number, Ulcer Index, and Preventive Index)
Exposing rats to pyloric-ligation/indomethacin administration (gastric ulcer control group) resulted in significant ulceration in the glandular part of their stomachs as it expressed elevations in ulcer number and ulcer index of 13.57 ± 2.89 and 29.71 ± 7.85, respectively, in comparison to the normal control group. Omeprazole pretreatment expressed a significant reduction in ulcer number and ulcer index, to 22.11% and 35.09%, respectively, in comparison to the gastric ulcer control group, providing 64.90% protection against gastric mucosal injury. Similarly, misoprostol pretreatment expressed a significant reduction in ulcer number and ulcer index, to 29.48% and 42.79%, respectively, in comparison to the gastric ulcer control group, providing 57.21% protection. In addition, Althaea officinalis pretreatment expressed a significant reduction in ulcer number and ulcer index, to 44.21% and 44.23%, respectively, in comparison to the gastric ulcer control group, providing 55.77% protection. Solanum nigrum pretreatment expressed a significant reduction in ulcer number and ulcer index, to about 37.89% and 44.71%, respectively, in comparison to the gastric ulcer control group, providing 55.29% protection ( Figure 3). Statistical analysis was performed via one-way ANOVA test, and after that the Tukey-Kramer multiple comparisons test was done. Where: * indicates a significant difference from ascorbic acid (p < 0.05), and @ indicates a significant difference from Althaea officinalis (p < 0.05).

Figure 2.
In vitro anti-oxidant activity of aqueous extracts of Althaea officinalis flower and ethanolic extracts of Solanum nigrum fruit. Each value signifies the mean ± standard error of the mean. Statistical analysis was performed via one-way ANOVA test, and after that the Tukey-Kramer multiple comparisons test was done. Where: * indicates a significant difference from ascorbic acid (p < 0.05), and @ indicates a significant difference from Althaea officinalis (p < 0.05).

Macroscopic Examination (Assessment of Gross Mucosal Damage) (Ulcer Number, Ulcer Index, and Preventive Index)
Exposing rats to pyloric-ligation/indomethacin administration (gastric ulcer control group) resulted in significant ulceration in the glandular part of their stomachs as it expressed elevations in ulcer number and ulcer index of 13.57 ± 2.89 and 29.71 ± 7.85, respectively, in comparison to the normal control group. Omeprazole pretreatment expressed a significant reduction in ulcer number and ulcer index, to 22.11% and 35.09%, respectively, in comparison to the gastric ulcer control group, providing 64.90% protection against gastric mucosal injury. Similarly, misoprostol pretreatment expressed a significant reduction in ulcer number and ulcer index, to 29.48% and 42.79%, respectively, in comparison to the gastric ulcer control group, providing 57.21% protection. In addition, Althaea officinalis pretreatment expressed a significant reduction in ulcer number and ulcer index, to 44.21% and 44.23%, respectively, in comparison to the gastric ulcer control group, providing 55.77% protection. Solanum nigrum pretreatment expressed a significant reduction in ulcer number and ulcer index, to about 37.89% and 44.71%, respectively, in comparison to the gastric ulcer control group, providing 55.29% protection ( Figure 3).

Routine H and E Staining (Histopathological Examination of Stomach)
As shown in Figure 4 and Table 2, normal rats' stomachs showed normal histological structures of gastric mucosa including glandular tissue, submucosa, muscular coat, and covering serosa without abnormal alterations (Figure 4a1,a2,a3). Exposing rats to pyloric-ligation/indomethacin administration (gastric ulcer control rats) demonstrated multiple ulcerations and necrotic foci in gastric mucosa with destructed cellular elements and extensive submucosal edema accompanied with congested and dilated blood vessels with many inflammatory cells infiltrations (Figure 4b1,b2,b3). Pretreatment with omeprazole showed apparent intact glandular mucosa. However, extensive submucosal edema is evident with congested blood vessels. Minimal inflammatory cells infiltrations were recorded (Figure 4c1,c2,c3). Similarly, misoprostol pretreatment is almost like the omeprazole (1st standard pretreatment) group records. However, scattered focal aggregations of inflammatory cells were recorded in deep mucosal and submucosal layers (Figure 4d1,d2,d3). In addition, Althaea officinalis pretreatment showed apparent intact glandular mucosa structures. Mild submucosal edemas with focal perivascular aggregation of inflammatory cells were observed in the subserosal layer ( Figure 4e1,e2,e3). Solanum nigrum pretreatment demonstrated apparent intact mucosa and glandular structures, extensive submucosal edema, congestions of blood vessels, and focal aggregation of mononuclear inflammatory cells (Figure 4f1,f2,f3).
pyloric-ligation/indomethacin-induced gastric-ulceration experimentally in male rats. Each value signifies the mean of 6-8 animals ± standard error of the mean. Statistical analysis was performed via a one-way ANOVA test, and after that a Tukey-Kramer multiple comparisons test was done. Where: (a) indicates a significant difference from normal control group (p < 0.05), and (b) indicates a significant difference from gastric ulcer control group (p < 0.05).

Routine H and E Staining (Histopathological Examination of Stomach)
As shown in Figure 4 and Table 2, normal rats' stomachs showed normal histological structures of gastric mucosa including glandular tissue, submucosa, muscular coat, and covering serosa without abnormal alterations (Figure 4a1,a2,a3). Exposing rats to pyloric-ligation/indomethacin administration (gastric ulcer control rats) demonstrated multiple ulcerations and necrotic foci in gastric mucosa with destructed cellular elements and extensive submucosal edema accompanied with congested and dilated blood vessels with many inflammatory cells infiltrations ( Figure  4b1,b2,b3). Pretreatment with omeprazole showed apparent intact glandular mucosa. However, extensive submucosal edema is evident with congested blood vessels. Minimal inflammatory cells infiltrations were recorded (Figure 4c1,c2,c3). Similarly, misoprostol pretreatment is almost like the omeprazole (1st standard pretreatment) group records. However, scattered focal aggregations of inflammatory cells were recorded in deep mucosal and submucosal layers (Figure 4d1,d2,d3). In addition, Althaea officinalis pretreatment showed apparent intact glandular mucosa structures. Mild submucosal edemas with focal perivascular aggregation of inflammatory cells were observed in the subserosal layer (

Alcian Blue Staining for Determination of Gastric Wall Mucus (Mucin Content)
Exposing rats to pyloric-ligation/indomethacin administration (gastric ulcer control group) expressed a significant reduction in mucin content of 2.9 ± 0.37% of alcian blue +ve mucin, in comparison to the normal control group. Omeprazole pretreatment significantly increased mucin content to 431.03% in comparison to the gastric ulcer control group. Misoprostol pretreatment significantly increased mucin content to 250.59%, in comparison to the gastric ulcer control group. It significantly decreased mucin content to 58.14%, in comparison to the omeprazole (1st standard pretreatment) group. In addition, Althaea officinalis pretreatment significantly increased mucin content to 211.21%, in comparison to the gastric ulcer control group. It significantly decreased mucin content to 49%, in comparison to the omeprazole (1st standard pretreatment) group. Solanum nigrum pretreatment significantly increased mucin content to 245.52%, in comparison to the gastric ulcer control group. It significantly decreased mucin content to 56.96%, in comparison to the omeprazole (1st standard pretreatment) group ( Figure 5). is edema, (red arrow) is congested or dilated blood vessels, and (dashed arrow) is inflammatory cell infiltrations. Exposing rats to pyloric-ligation/indomethacin administration (gastric ulcer control group) expressed a significant reduction in mucin content of 2.9 ± 0.37% of alcian blue +ve mucin, in comparison to the normal control group. Omeprazole pretreatment significantly increased mucin content to 431.03% in comparison to the gastric ulcer control group. Misoprostol pretreatment significantly increased mucin content to 250.59%, in comparison to the gastric ulcer control group. It significantly decreased mucin content to 58.14%, in comparison to the omeprazole (1st standard pretreatment) group. In addition, Althaea officinalis pretreatment significantly increased mucin content to 211.21%, in comparison to the gastric ulcer control group. It significantly decreased mucin content to 49%, in comparison to the omeprazole (1st standard pretreatment) group. Solanum nigrum pretreatment significantly increased mucin content to 245.52%, in comparison to the gastric ulcer control group. It significantly decreased mucin content to 56.96%, in comparison to the omeprazole (1st standard pretreatment) group ( Figure 5). Each value signifies the mean of 6-8 animals ± standard error of the mean. Statistical analysis was performed via one-way ANOVA test, and after that the Tukey-Kramer multiple comparisons test was done. Where: (a) indicates a significant difference from the normal control group (p < 0.05), (b) indicates a significant difference from the gastric ulcer control group (p < 0.05), and (c) indicates a significant difference from the omeprazole (1st standard pretreatment) group (p < 0.05).
3.5. Acid Productivity Parameters (Gastric Volume, Titratable Acidity, and Acid Output Determination) Exposing rats to pyloric-ligation/indomethacin administration (gastric ulcer control group) resulted in a significant increase in gastric volume and acid output of 4.29 ± 0.36 mL/4 h and 112.33 ± 11.73 µEq/h, respectively, in comparison to 0.18 ± 0.04 mL/4 h and 10.5 ± 0 µEq/h, respectively, for the normal control group. Alternatively, induction caused a reduction in titratable acidity, reaching a value of 105 ± 5.40 mEq/L in comparison to 140 ± 0 mEq/L for the normal control group.
The standard and test pretreatment groups showed significantly increased gastric volume and acid output in comparison to the normal control group, but they had a reduction in titratable acidity in comparison to the normal control group.
Omeprazole pretreatment caused change (but not significantly) in gastric volume, titratable acidity, or acid output to 4.17 ± 0.24 mL/4 h, 88.75 ± 6.45 mEq/L, and 90.98 ± 4.50 µEq/h, respectively, in comparison to the gastric ulcer control group. Similarly, misoprostol pretreatment caused change (but not significantly) in gastric volume, titratable acidity, or acid output to 3.91 ± 0.37 mL/4 h, 93.57 ± 10.55 mEq/L, and 88.74 ± 9.05 µEq/h, respectively, in comparison to the gastric ulcer control group. In addition, Althaea officinalis pretreatment caused change (but not significantly) in gastric volume, titratable acidity, or acid output to 5 ± 0.96 mL/4 h, 74.17 ± 14.63 mEq/L, and 90.76 ± 17.19 µEq/h, respectively, in comparison to the gastric ulcer control group. Solanum nigrum pretreatment caused change (but not significantly) in gastric volume, titratable acidity, or acid output to 3.53 ± 0.36 mL/4 h, 121 ± 16.21 mEq/L, and 109.69 ± 13.50 µEq/h, respectively, in comparison to the gastric ulcer control group. Also, the test pretreatment groups did not significantly change gastric volume, titratable acidity, or acid output in comparison to the omeprazole (1st standard pretreatment) group or the misoprostol (2nd standard pretreatment) group ( Figure 6).  Exposing rats to pyloric-ligation/indomethacin administration (gastric ulcer control group) expressed a significant elevation in peptic activity, histamine, and gastrin contents as they were 28.77 ± 0.62 mg/mL, 28.13 ± 0.93 ng/g, and 15.85 ± 0.64 Pg/mL, respectively, in comparison to 20.27 ± 2.49 mg/mL, 2.23 ± 0.14 ng/g, and 1.33 ± 0.09 Pg/mL, respectively, for the normal control group. Omeprazole pretreatment did not significantly affect peptic activity as it was 31.39 ± 1.47 mg/mL in comparison to the gastric ulcer control group. But, it expressed a significant reduction in histamine and gastrin contents to 24% and 23.5%, respectively, in comparison to the gastric ulcer control group. Similarly, misoprostol pretreatment did not significantly affect peptic activity as it was 29.50 ± 1.55 mg/mL in comparison to the the gastric ulcer control group. But, it expressed a significant reduction in histamine and gastrin contents to 50.49% and 46.85%, respectively, in comparison to the gastric ulcer control group. Also, it expressed a significant elevation in histamine and gastrin contents to 210.37% and 199.33%, respectively, in comparison to the omeprazole (1st standard pretreatment) group. In addition, Althaea officinalis pretreatment expressed a significant reduction in peptic activity, histamine, and gastrin contents to 67.08%, 48.53%, and 45.27%, respectively, in comparison to the gastric ulcer control group and it expressed a significant reduction in peptic activity to 61.48%, in comparison to the omeprazole (1st standard pretreatment) group. Also, it expressed a significant reduction in peptic activity to 65.42% in comparison to the misoprostol (2nd standard treatment) group. It expressed a significant elevation in histamine and gastrin contents to 202.22% and 192.62%, respectively, in comparison to the omeprazole (1st standard pretreatment) group. Solanum nigrum pretreatment did not significantly affect peptic activity as it was 27.45 ± 1.82 mg/mL in comparison to the gastric ulcer control group. But, it expressed a significant reduction in histamine and gastrin contents to 44.27% and 41.17%, respectively, in comparison to the gastric ulcer control group. Also, it expressed a significant elevation in histamine and gastrin contents to 184.44% and 175.17%, respectively, in comparison to the omeprazole (1st standard pretreatment) group. Also, it expressed a significant elevation in peptic activity to 142.23% in comparison to the Althaea officinalis (1st test pretreatment) treated group (Figure 7).

Oxidative Stress Biomarkers (Gastric Mucosal NO, GSH, and MDA Contents and SOD Activity)
Exposing rats to pyloric-ligation/indomethacin administration (gastric ulcer control group) expressed a significant reduction in GSH and NO contents and SOD activity as it was 2.83 ± 0.34 Pg/g, 2.40 ± 0.26 µmol/g and 1.58 ± 0.17 U/g, respectively, in comparison to 51.98 ± 2.45 Pg/g, 41.63 ± 1.95 µmol/g, and 25.38 ± 1.04 U/g, respectively, for the normal control group and it expressed a significant elevation in MDA content to 30.50 ± 1.22 ng/g, in comparison to 2.75 ± 0.17 ng/g for the normal control group. Omeprazole pretreatment expressed a significant elevation in GSH and NO contents and SOD activity to 1014.16%, 962.50%, and 892.06%, respectively, in comparison to the gastric ulcer control group. It expressed a significant reduction in MDA content to 24.51% in comparison to the gastric ulcer control group. Misoprostol pretreatment expressed a significant elevation in GSH and NO contents and SOD activity to 624.78%, 589.58%, and 580.95%, respectively, in comparison to the gastric ulcer control group. It expressed a significant reduction in GSH and NO contents and SOD activity to 61.61%, 61.26%, and 65.13%, respectively, in comparison to the omeprazole (1st standard pretreatment) group. It expressed a significant reduction in MDA content to 48.85% in comparison to the gastric ulcer control group. It expressed a significant elevation in MDA content to 199.33% in comparison to the omeprazole (1st standard pretreatment) group. In addition, Althaea officinalis pretreatment expressed a significant elevation in GSH and NO contents and SOD activity to 617.70%, 602.08%, and 561.91%, respectively, in comparison to the gastric ulcer control group. It expressed a significant reduction in GSH and NO contents and SOD activity to 60.91%, 62.55%, and 62.99%, respectively, in comparison to the omeprazole (1st standard pretreatment) group. It expressed a significant reduction in MDA content to 47.29% in comparison to the gastric ulcer control group. It expressed a significant elevation in MDA content to 192.98% in comparison to the omeprazole (1st standard pretreatment) group. Solanum nigrum pretreatment expressed a significant elevation in GSH and NO contents and SOD activity to 617.70%, 589.58%, and 579.37%, respectively, in comparison to the gastric ulcer control group. It expressed a significant reduction in GSH and NO contents and SOD activity to 60.91%, 61.26%, and 64.95%, respectively, in comparison to the omeprazole (1st standard pretreatment) group. It expressed a significant reduction in MDA content to 42.79% in comparison to the gastric ulcer control group. It expressed a significant elevation in MDA content to 174.58% in comparison to the omeprazole (1st standard pretreatment) group (Figure 8). , and gastrin (C) against pyloric-ligation/indomethacin-induced gastric-ulceration experimentally in male rats. Each value signifies the mean of 6-8 animals ± standard error of the mean. Statistical analysis was performed via one-way ANOVA test, and after that the Tukey-Kramer multiple comparisons test was done. Where: (a) indicates a significant difference from the normal control group (p < 0.05), (b) indicates a significant difference from the gastric ulcer control group (p < 0.05), (c) indicates a significant difference from the omeprazole (1st standard pretreatment) group (p < 0.05), and (d) indicates a significant difference from the misoprostol (2nd standard pretreatment) group (p < 0.05).

Oxidative Stress Biomarkers (Gastric Mucosal NO, GSH, and MDA Contents and SOD Activity)
Exposing rats to pyloric-ligation/indomethacin administration (gastric ulcer control group) expressed a significant reduction in GSH and NO contents and SOD activity as it was 2.83 ± 0.34 Pg/g, 2.40 ± 0.26 µmol/g and 1.58 ± 0.17 U/g, respectively, in comparison to 51.98 ± 2.45 Pg/g, 41.63 ± 1.95 µmol/g, and 25.38 ± 1.04 U/g, respectively, for the normal control group and it expressed a significant elevation in MDA content to 30.50 ± 1.22 ng/g, in comparison to 2.75 ± 0.17 ng/g for the normal control group. Omeprazole pretreatment expressed a significant elevation in GSH and NO contents and SOD activity to 1014.16%, 962.50%, and 892.06%, respectively, in comparison to the gastric ulcer control group. It expressed a significant reduction in MDA content to 24.51% in comparison to the gastric ulcer control group. Misoprostol pretreatment expressed a significant elevation in GSH and NO contents and SOD activity to 624.78%, 589.58%, and 580.95%, respectively, in comparison to the gastric ulcer control group. It expressed a significant reduction in GSH and NO contents and SOD activity to 61.61%, 61.26%, and 65.13%, respectively, in comparison to the reduction in GSH and NO contents and SOD activity to 60.91%, 61.26%, and 64.95%, respectively, in comparison to the omeprazole (1st standard pretreatment) group. It expressed a significant reduction in MDA content to 42.79% in comparison to the gastric ulcer control group. It expressed a significant elevation in MDA content to 174.58% in comparison to the omeprazole (1st standard pretreatment) group (Figure 8).

Protective Factors (Gastric Mucosal PG-E2 and PG-I2 Contents)
Exposing rats to pyloric-ligation/indomethacin administration (gastric ulcer control group) expressed a significant reduction in PG-E2 content as it was 1.98 ± 0.16 Pg/g in comparison to 38.65 ± 1.62 Pg/g for the normal control group. Omeprazole pretreatment expressed a significant elevation in PG-E2 content to 1022.78% in comparison to the gastric ulcer control group. Misoprostol pretreatment expressed a significant elevation in PG-E2 content to 564.56% in comparison to the gastric ulcer control group. It expressed a significant reduction in PG-E2 content to 55.2% in comparison to the omeprazole (1st standard pretreatment) group. In addition, Althaea officinalis pretreatment expressed a significant elevation in PG-E2 content to 578.48% in comparison to the gastric ulcer control group. It expressed a significant reduction in PG-E2 content to 56.56% in comparison to the omeprazole (1st standard pretreatment) group. Solanum nigrum pretreatment Figure 8. Protective effects of Althaea officinalis and Solanum nigrum with standards misoprostol and omeprazole on nitric oxide "NO" (A), glutathione "GSH" (B), superoxide-dismutase "SOD" (C), and malondialdehyde "MDA" (D) against pyloric-ligation/indomethacin-induced gastric-ulceration experimentally in male rats. Each value signifies the mean of 6-8 animals ± standard error of the mean. Statistical analysis was performed via one-way ANOVA test, and after that the Tukey-Kramer multiple comparisons test was done. Where: (a) indicates a significant difference from the normal control group (p < 0.05), (b) indicates a significant difference from the gastric ulcer control group (p < 0.05), and (c) indicates a significant difference from the omeprazole (1st standard pretreatment) group (p < 0.05).

Protective Factors (Gastric Mucosal PG-E 2 and PG-I 2 Contents)
Exposing rats to pyloric-ligation/indomethacin administration (gastric ulcer control group) expressed a significant reduction in PG-E 2 content as it was 1.98 ± 0.16 Pg/g in comparison to 38.65 ± 1.62 Pg/g for the normal control group. Omeprazole pretreatment expressed a significant elevation in PG-E 2 content to 1022.78% in comparison to the gastric ulcer control group. Misoprostol pretreatment expressed a significant elevation in PG-E 2 content to 564.56% in comparison to the gastric ulcer control group. It expressed a significant reduction in PG-E 2 content to 55.2% in comparison to the omeprazole (1st standard pretreatment) group. In addition, Althaea officinalis pretreatment expressed a significant elevation in PG-E 2 content to 578.48% in comparison to the gastric ulcer control group. It expressed a significant reduction in PG-E 2 content to 56.56% in comparison to the omeprazole (1st standard pretreatment) group. Solanum nigrum pretreatment expressed a significant elevation in PG-E 2 content to 573.42% in comparison to the gastric ulcer control group. It expressed a significant reduction in PG-E 2 content to 56.06% in comparison to the omeprazole (1st standard pretreatment) group.
The standard and test pretreatment groups increased PG-I 2 content to different values in comparison to the gastric ulcer control group Exposing rats to pyloric-ligation/indomethacin administration (gastric ulcer control group) showed a significant decrease in PG-I 2 content as it was 79.68 ± 2.59 Pg/g in comparison to 120.53 ± 5.48 Pg/g for the normal control group. Pretreatment with omeprazole, misoprostol, Althaea officinalis, and Solanum nigrum increased (but not significantly) PG-I 2 content as it was 97.93 ± 4.28 Pg/g, 89.85 ± 2.32 Pg/g, 99.23 ± 5.66 Pg/g, and 102.98 ± 8.31 Pg/g, respectively, in comparison to the gastric ulcer control group (Figure 9). showed a significant decrease in PG-I2 content as it was 79.68 ± 2.59 Pg/g in comparison to 120.53 ± 5.48 Pg/g for the normal control group. Pretreatment with omeprazole, misoprostol, Althaea officinalis, and Solanum nigrum increased (but not significantly) PG-I2 content as it was 97.93 ± 4.28 Pg/g, 89.85 ± 2.32 Pg/g, 99.23 ± 5.66 Pg/g, and 102.98 ± 8.31 Pg/g, respectively, in comparison to the gastric ulcer control group (Figure 9).
(A) (B) Figure 9. Protective effects of Althaea officinalis and Solanum nigrum with standards misoprostol and omeprazole on prostaglandin-E2 "PG-E2" (A) and prostacyclin "PG-I2" (B) against pyloric-ligation/indomethacin-induced gastric-ulceration experimentally in male rats. Each value signifies the mean of 6-8 animals ± standard error of the mean. Statistical analysis was performed via one-way ANOVA test, and after that the Tukey-Kramer multiple comparisons test was done. Where: (a) indicates a significant difference from the normal control group (p < 0.05), (b) indicates a significant difference from the gastric ulcer control group (p < 0.05), and (c) indicates a significant difference from the omeprazole (1st standard pretreatment) group (p < 0.05).

Immunological Markers (Gastric Mucosal TNF-α and IL-1β Contents and CBS and HO-1 Activities) by Western Blot Analysis
Gastric mucosal TNF-α and IL-1β contents in the normal control group was 1 ± 0 and 1.01 ± 0.01, respectively, and gastric mucosal CBS and HO-1 activities in the normal control group was 1.01 ± 0.01 and 1.01 ± 0.01, respectively. Exposing rats to pyloric-ligation/indomethacin administration showed a significant increase in TNF-α and IL-1β contents to 847.5% and 763.89%, respectively, in comparison to normal control rats. On other hand, it showed a significant decrease in CBS and HO-1 activities to 22.12% and 32.14%, respectively, in comparison to the normal control group. Omeprazole pretreatment significantly decreased TNF-α and IL-1β contents to 59.88% and 48.38%, respectively, in comparison to the gastric ulcer control. It did not significantly affect CBS activity as it was 0.39 ± 0.08. But it significantly increased HO-1 activity to 185.76% in comparison to the gastric ulcer control group. Misoprostol pretreatment significantly decreased TNF-α and IL-1β contents to 46.90% and 40.26%, respectively, in comparison to the gastric ulcer control group. On other hand, it did not significantly affect CBS activity as it was 0.46 ± 0.06. But it significantly increased HO-1 activity to 198.142% in comparison to the gastric ulcer control group. Additionally, Althaea officinalis pretreatment significantly decreased TNF-α and IL-1β contents to 30.38% and 34.42%, respectively, in comparison to the gastric ulcer control group. But, it significantly increased CBS and HO-1 activities to 266.82% and 187.31%, respectively, in comparison to the gastric ulcer control group. Solanum nigrum pretreatment significantly decreased TNF-α and IL-1β contents to 37.76% and 42.53%, respectively, in comparison to the gastric ulcer control group. It significantly increased CBS and HO-1 activities to 271.3% and 230.65%, respectively, in comparison to the gastric ulcer control Figure 9. Protective effects of Althaea officinalis and Solanum nigrum with standards misoprostol and omeprazole on prostaglandin-E 2 "PG-E 2 " (A) and prostacyclin "PG-I 2 " (B) against pyloric-ligation/indomethacin-induced gastric-ulceration experimentally in male rats. Each value signifies the mean of 6-8 animals ± standard error of the mean. Statistical analysis was performed via one-way ANOVA test, and after that the Tukey-Kramer multiple comparisons test was done. Where: (a) indicates a significant difference from the normal control group (p < 0.05), (b) indicates a significant difference from the gastric ulcer control group (p < 0.05), and (c) indicates a significant difference from the omeprazole (1st standard pretreatment) group (p < 0.05).

Immunological Markers (Gastric Mucosal TNF-α and IL-1β Contents and CBS and HO-1 Activities) Estimated by Western Blot Analysis
Gastric mucosal TNF-α and IL-1β contents in the normal control group was 1 ± 0 and 1.01 ± 0.01, respectively, and gastric mucosal CBS and HO-1 activities in the normal control group was 1.01 ± 0.01 and 1.01 ± 0.01, respectively. Exposing rats to pyloric-ligation/indomethacin administration showed a significant increase in TNF-α and IL-1β contents to 847.5% and 763.89%, respectively, in comparison to normal control rats. On other hand, it showed a significant decrease in CBS and HO-1 activities to 22.12% and 32.14%, respectively, in comparison to the normal control group. Omeprazole pretreatment significantly decreased TNF-α and IL-1β contents to 59.88% and 48.38%, respectively, in comparison to the gastric ulcer control. It did not significantly affect CBS activity as it was 0.39 ± 0.08. But it significantly increased HO-1 activity to 185.76% in comparison to the gastric ulcer control group. Misoprostol pretreatment significantly decreased TNF-α and IL-1β contents to 46.90% and 40.26%, respectively, in comparison to the gastric ulcer control group. On other hand, it did not significantly affect CBS activity as it was 0.46 ± 0.06. But it significantly increased HO-1 activity to 198.142% in comparison to the gastric ulcer control group. Additionally, Althaea officinalis pretreatment significantly decreased TNF-α and IL-1β contents to 30.38% and 34.42%, respectively, in comparison to the gastric ulcer control group. But, it significantly increased CBS and HO-1 activities to 266.82% and 187.31%, respectively, in comparison to the gastric ulcer control group. Solanum nigrum pretreatment significantly decreased TNF-α and IL-1β contents to 37.76% and 42.53%, respectively, in comparison to the gastric ulcer control group. It significantly increased CBS and HO-1 activities to 271.3% and 230.65%, respectively, in comparison to the gastric ulcer control group. It significantly increased HO-1 activity to 124.17%, in comparison to the omeprazole (1st standard pretreatment) group ( Figure 10). Solanum nigrum extracts compared to standards misoprostol and omeprazole on tumor-necrosis-factor-alpha "TNF-α" (B), interleukin-1-beta "IL-1β" (C), heme-oxygenase-1 "HO-1" (D), and cystathionine-β-synthase "CBS" (E) against pyloric-ligation/indomethacin-induced gastric-ulceration experimentally in male rats. Each value signifies the mean of 6-8 animals ± standard error of the mean. Statistical analysis was performed via one-way ANOVA test, and after that the Tukey-Kramer multiple comparisons test was done. Where: (a) indicates a significant difference from the normal control group (p < 0.05), (b) indicates a significant difference from the gastric ulcer control group (p < 0.05), and (c) indicates a significant difference from the omeprazole (1st standard pretreatment) group (p < 0.05).

Discussion
In the present study, the gastro-protective activity and the anti-oxidant effect of Althaea officinalis and Solanum nigrum extracts was investigated in pyloric-ligation/indomethacin-induced gastric-ulceration in male rats and their possible underlying mechanisms included in their actions were investigated.
Preliminary phytochemical analysis of test extracts (aqueous extracts of Althaea officinalis and ethanolic extracts of Solanum nigrum) was accomplished prior to the pharmacological study. The Althaea officinalis flower extract was positive for carbohydrates, traces of tannins, flavonoids, traces of saponins, and volatile oils. In agreement, Al-Snafi [60] has documented similar results. Solanum Figure 10.
Western blot analysis (A) and figure showing protective effects of Althaea officinalis and Solanum nigrum extracts compared to standards misoprostol and omeprazole on tumor-necrosis-factor-alpha "TNF-α" (B), interleukin-1-beta "IL-1β" (C), heme-oxygenase-1 "HO-1" (D), and cystathionine-β-synthase "CBS" (E) against pyloric-ligation/indomethacin-induced gastric-ulceration experimentally in male rats. Each value signifies the mean of 6-8 animals ± standard error of the mean. Statistical analysis was performed via one-way ANOVA test, and after that the Tukey-Kramer multiple comparisons test was done. Where: (a) indicates a significant difference from the normal control group (p < 0.05), (b) indicates a significant difference from the gastric ulcer control group (p < 0.05), and (c) indicates a significant difference from the omeprazole (1st standard pretreatment) group (p < 0.05).

Discussion
In the present study, the gastro-protective activity and the anti-oxidant effect of Althaea officinalis and Solanum nigrum extracts was investigated in pyloric-ligation/indomethacin-induced gastric-ulceration in male rats and their possible underlying mechanisms included in their actions were investigated.
Preliminary phytochemical analysis of test extracts (aqueous extracts of Althaea officinalis and ethanolic extracts of Solanum nigrum) was accomplished prior to the pharmacological study. The Althaea officinalis flower extract was positive for carbohydrates, traces of tannins, flavonoids, traces of saponins, and volatile oils. In agreement, Al-Snafi [60] has documented similar results. Solanum nigrum fruit extract was positive for carbohydrates, tannins, flavonoids, saponins, sterols and triterpenoids and alkaloids, in agreement with the results obtained by El-Hawary et al. and Rani et al. [37,61].
The inhibition of gastric ulcers induced by indomethacin is believed to be mediated by anti-oxidant effects [8,62]. Free radical scavenging drugs, either from synthetic or herbal sources, have an important role in decreasing, de-activating, and eliminating reactive oxygen species (ROS). Accordingly, these drugs exert a significant protection for tissues from oxidative damage as well as enhancement of wound healing [63]. Many previous investigations documented the major role of oxidative stress in the pathogenesis of many diseases, including gastric ulcers [64]. Oxidative stress is considered one of the major causative factors of gastric ulcers. In the normal state, reactive oxygen species (ROS) are always produced, but eliminated by anti-oxidant defense mechanisms of the body. Imbalance in the anti-oxidant defense system resulted in the generation of gastric damage [65]. Oxidative damage, cell death, and epithelial damage resulted from increased ROS. Additionally, it was documented that ROS expressed an elevation in gastric acid secretion by releasing histamine and reducing mucus content by diminishing prostaglandin synthesis [33].
Aqueous extracts of Althaea officinalis and ethanolic extracts of Solanum nigrum showed free radical scavenging activities against DPPH. The results of the in vitro anti-oxidant activity of test extracts were in agreement with previous studies [32,60,[66][67][68]. Ethanolic extracts of Solanum nigrum showed the highest anti-oxidant effect, exhibiting the smallest IC 50 values. Alternatively, the aqueous extract of Althaea officinalis was the least active by showing the highest IC 50 values.
Non-steroidal anti-inflammatory drugs (NSAIDs) are considered one of the most frequently used medication classes worldwide. NSAIDs administration represents the main reason for gastric-ulceration [18]. Indomethacin, in particular, is commonly used and considered a clinically appropriate experimental model for gastric ulcer induction. Mechanistically, indomethacin generates gastric ulcers by inhibiting prostaglandin synthesis, generating reactive oxygen species (ROS) production, initiating lipid peroxidation formation [8,69], prompting apoptosis and necrosis of stomach cells [70,71], reducing bicarbonate and mucus secretion, increasing gastric motility [72], elevating pro-inflammatory cytokines production [73], and finally, disrupting nitric oxide production in gastric tissues [74,75]. The ulcerogenic mechanism of indomethacin is complex and remains unclear [76]. Briefly, indomethacin-induced gastric-ulceration was suggested to be caused by inhibiting the release of protective factors, accumulating aggressive factors, and aggravating oxidant parameters while diminishing anti-oxidant parameters [8,77]. Accordingly, the use of drugs with anti-oxidant activity, and the capability to modify nitric oxide content, can protect against indomethacin-induced gastric-ulceration [75].
Pyloric-ligation induced gastric-ulceration resulted from auto-digestion and break down of the stomach wall [78] by the digestive effect of accumulated gastric juice (gastric acid and pepsin), interference of gastric blood circulation [33,79], and increased production of free radicals [12]. Drugs which express a reduction in gastric juice secretion and elevation in mucus production can prevent the pyloric-ligation induced gastric-ulcerations [80].
Proton-pump inhibitors (PPIs) are often co-administered with NSAIDs to reduce NSAID-induced gastrointestinal (GI) adverse events. This co-administration is generally regarded as safe, and is included in many of the guidelines on NSAID prescription [81].
Misoprostol had been used in many investigations as a positive control drug [82]. Previously, Adinortey et al. reported that it is advisable to compare the potential drug or test material with cytoprotectant reference drugs such as misoprostol that are known to prevent peptic ulcers [22]. Misoprostol is effective but its side effects limit its use [21]. Misoprostol protects the GI mucosa by stimulating mucus/bicarbonate secretion [81].
Some herbs have shown inhibition of gastric mucosal damage induced experimentally by necrotizing agents through their anti-secretory and anti-oxidant properties [21].
Althaea officinalis is traditionally used as a treatment for the irritation of mucous membranes, including use as a gargle for mouth and throat ulcers and gastric ulcers [60,83]. Althaea officinalis flower is commonly used in folk medicine in Middle East countries. This flower contains a variety of bioflavonoids, vitamins, and anti-oxidant compounds [35]. The extract of Althaea officinalis exhibited strong anti-oxidant activity in different anti-oxidant tests [32]. The aqueous extract of Althaea officinalis was demonstrated to be potentially helpful in treating gastric ulcers with no visible adverse effects [25,34,35,84]. The observed gastro-protective effect of Althaea officinalis could be attributed to active phytoconstituents found in the extract such as flavonoids and mucilage polysaccharides [25]. Althaea officinalis has a mucus protection (cytoprotection effect) and an anti-oxidant effect [85]. These results are also supported by and continuous to our previous research on aqueous extracts of Althaea officinalis flowers [86].
Solanum nigrum is a herbal plant that has been traditionally used in oriental medicines and is believed to have various biological activities [5,66,67,87]. Solanum nigrum is currently used in Egyptian folk medicine for some of gastro-intestinal tract (GIT) disorders [17]. Although Solanum nigrum is a rich source of one of the plants' most dreaded toxins [88], the fruit of Solanum nigrum is reported to have anti-oxidant, cytoprotective, anti-ulcerogenic, ulcer healing, anti-inflammatory, and anti-tumor effects in rats [1,67,87]. Additionally, it is a potential herbal alternative that acts as an anti-cancer agent [61]. Many researchers have proven that anti-oxidants may play an important role not only by protecting against gastric mucosal injury, but also by inhibiting progression of gastric ulcers. The observed cytoprotective and anti-oxidative activity of Solanum nigrum extract is attributed to the presence of biologically active phytoconstituents having anti-oxidative nature [5].
Results of the current investigation revealed that pyloric ligation of rat stomachs followed by administration of indomethacin (25 mg/kg, i.p.) caused significant ulceration in the glandular area and degenerative changes in gastric tissues of the rat stomach as seen in histopathological examination as it showed epithelial loss, congested blood vessels with inflammatory cell infiltration, associated with considerable elevation in ulcer number and ulcer index, which was in agreement with previous studies that indicated that NSAIDs like indomethacin can generate observable gastric ulcers in experimental animals [1,6,18,39,75,89,90]. Pyloric-ligation/indomethacin-induced mucosal injury is attributed to various processes, including infiltration of leukocytes, inhibition of PG-E 2 , initiation of lipid peroxidation, decreasing levels of anti-oxidants, and induction of apoptosis [91,92].
Pretreatment with omeprazole protected animals from pyloric-ligation/indomethacin-induced gastric-ulceration as manifested by significantly reduced ulcer number and ulcer index. Similar results were obtained by Bhalke et al., who demonstrated that omeprazole in a dose of 20 mg/kg produced a significant reduction in the ulcer index and has high protection against aspirin-induced gastric ulcers and produced protection against the pylorus-ligation induced gastric ulcers model [7]. In another study, omeprazole for three days reduced the number of inflammatory cells and mucosal congestion, and increased the number of healthy normal cells in the gastric mucosa, submucosa, serosa, and muscle layers [93]. The reference drug omeprazole showed protection against aspirin and pyloric-ligation induced ulcer models [94].
Additionally, misoprostol protected animals from pyloric-ligation/indomethacin-induced gastric-ulceration as manifested by significantly reduced ulcer number and ulcer index. Similar results were obtained by Bhalke et al., who demonstrated that misoprostol has shown a significant effect on the indomethacin-induced ulcer model with a high protection index [7]. Moreover, misoprostol administered before indomethacin significantly reduced the mean ulcer index in comparison to the mean ulcer index value of indomethacin intoxicated rats [95]. In agreement with our results, the histopathological examination of the stomach in the rats treated by misoprostol before indomethacin showed normal mucosal membrane adjacent to areas of gastritis [95].
Additionally, Althaea officinalis extract pretreatment showed anti-ulcer activity as observed by a significant reduction of the ulcer number and ulcer index after pyloric-ligation/indomethacin administration. In a previous study, it was shown that mucilage and flavonoids have the property of covering and protecting the gastric mucosa, thereby reducing the incidence of gastric ulcers [25]. Animals receiving Althaea officinalis extract showed a dose-dependent protection against ethanol-induced ulcers [96].
According to our study, Solanum nigrum extract significantly reduced the ulcer number and ulcer index after pyloric-ligation/indomethacin administration. Earlier, it was demonstrated that the aerial parts of Solanum nigrum were reported to decrease the ulcer index significantly [17]. In addition, Awaad et al. reported that oral treatment with the methanol extract of Solanum nigrum significantly inhibited the gastric lesions [24]. Earlier, it has been reported earlier that Solanum nigrum could offer an anti-ulcer action as it significantly inhibited the gastric lesions induced by cold restraint, indomethacin, pyloric-ligation, and ethanol. The reduction of ulcer size by Solanum nigrum was evident by histological findings [1]. Moreover, Solanum nigrum administered orally caused a dose-dependent decrease in the ulcer index and could inhibit the increase in area of gastric mucosal lesions in aspirin-induced ulceration in rats [5]. The Solanum nigrum extract decreased the mean ulcer index and proved its protective effect against ulcer formation due to pylorus-ligation [97].
Gastric mucus is the first guard against gastric acid and other ulcerogens. Mucus and bicarbonate together adhere to the epithelium working as a barrier against auto-digestion. The gastric mucus barrier has a crucial role in gastric ulcer protection. The ulcerogenic substances induce dissipation of the mucus gel layer and thus cause ulceration [26]. Previously, it was documented that gastric mucus has anti-oxidant activity, thereby reducing gastric-ulceration mediated by oxygen free radicals [19].
Our results showed that the induction of gastric ulceration by pyloric-ligation/indomethacin administration resulted in a significant decrease in mucin content in comparison to normal control rats. This result is in accordance with that obtained by previous researches [18,98].
Pretreatment with omeprazole significantly increased mucin content in comparison to the gastric ulcer control group. Similar results were obtained by previous studies [94,99]. Interestingly, animals pretreated with omeprazole showed preserved gastric mucus levels [100]. Omeprazole, a classic anti-ulcer drug, is known as not only a proton pump inhibitor, but also as a stimulator of gastric mucus secretion [8].
Misoprostol pretreatment significantly increased mucin content in comparison to the gastric ulcer control group, which was in harmony with investigations reporting that misoprostol administration before indomethacin-induced gastric-ulcerations produced an increase in mucin production [95].
In addition, Althaea officinalis pretreatment significantly increased mucin content in comparison to the gastric ulcer control group. Previously, the clinically proven effects were related to the presence of bioadhesive and mucilaginous polysaccharides, leading to the physical formation of a mucin-like substance on top of the irritated tissues [84].
Meanwhile, our results revealed that Solanum nigrum pretreatment significantly increased mucin content.
Acidity has a considerable role in the genesis of indomethacin-induced gastric-ulceration. Indomethacin-induced gastric-ulceration is magnified by high acid concentration in the stomach [101]. Histamine release results in the elevation of gastric juice secretion [18]. Gastrin is a hormone that can regulate gastric acid secretion, releases histamine, and regulates gastric endocrine cell proliferation. Over-secretion of gastrin hormone may induce hyper-secretion of acid by the parietal cells, causing gastric ulcers [1].
Significant increases in the gastric volume and acid output were noticed in ulcerated rats. Similar results have been reported by Inas et al. [18]. In addition, it was concluded that pyloric-ligation of rats for 4 h resulted in accumulation of gastric secretory volume [7]. A similar result was obtained by Adewoya and Salami who stated that indomethacin blocks synthesis of prostaglandins leading to an increased gastric acid secretion [99]. But, our results demonstrate that titratable acidity was not affected by gastric-ulceration induction.
Omeprazole did not significantly affect gastric volume, titratable acidity, or acid output in comparison to the gastric ulcer control group after pyloric-ligation/indomethacin-induced gastric-ulceration. In contrast, previous studies demonstrated that omeprazole showed a significant reduction in concentration and output of acid and pepsin secretion in pylorus-ligated rats [1]. Additionally, PPIs are known to suppress not only acid secretion but also the volume of gastric juice [102]. Moreover, the standard omeprazole treated group showed titratable acidity which is less than that of the negative (ulcer) control group [7].
Misoprostol pretreatment did not significantly change gastric volume, titratable acidity, or acid output in comparison to the gastric ulcer control group. These results were in contrast to the research which reported that misoprostol produced an increase in bicarbonate and decreased acid secretion [95,103].
Meanwhile, Althaea officinalis pretreatment did not significantly change gastric volume, titratable acidity, or acid output in comparison to the gastric ulcer control group.
Additionally, Solanum nigrum pretreatment did not significantly change gastric volume, titratable acidity, acid output, or peptic activity in comparison to the gastric ulcer control group. In contrast, previous investigations demonstrated that Solanum nigrum were reported to inhibit acid and pepsin secretions [17]. In another study, Solanum nigrum extract showed concomitant attenuation of gastric secretory volume, acidity, and pepsin secretion in ulcerated rats as in the 4 hrs pylorus-ligated rats, it decreased the gastric juice volume and reversed the increased output of acid and pepsin secretion [1].
In shay rats, administration of indomethacin (25 mg/kg, i.p.) showed an increase in peptic activity in agreement with results reported by Inas et al. and Malash et al. [18,98]. Our results showed that pyloric-ligation/indomethacin administration significantly increased gastric tissue histamine content. These results are in harmony with previous investigations concluding that indomethacin blocks synthesis of prostaglandins leading to an increased parietal cell number [99]. Additionally, gastric ulcer control rats showed a significant increase in gastrin content in comparison to normal control rats. This result is in accordance with that obtained by Jainu and Devi who demonstrated that ethanol treatment (ulcerated rats) induced a significant increase in the concentrations of plasma gastrin hormone [1].
Pretreatment with omeprazole did not significantly affect peptic activity in comparison to the gastric ulcer control group. In contrast, Kumar et al. and Jainu and Devi demonstrated that omeprazole caused significant reduction in concentration and output of pepsin secretion in pylorus-ligated rats [1,104]. Pretreatment with omeprazole was found to reduce pepsin concentration to near normal levels [105]. Anti-ulcer drugs, like ranitidine and omeprazole, act by an anti-secretory mechanism via inhibition of gastric secretion and pepsin activity [80]. Additionally, omeprazole significantly decreased histamine content in comparison to the gastric ulcer control group. Lansoprazole showed significant inhibition of H + /K + -ATPase activity when compared to rats that received ethanol [1]. A significant decrease in the parietal cell count of omeprazole pretreated rats was observed when compared with ulcerated untreated rats [99]. In addition, omeprazole significantly decreased gastrin content in comparison to the gastric ulcer control group. Pretreatment with lansoprazole caused a reduction in gastrin secretion in animals as compared with ethanol-ulcerated animals [1].
In addition, misoprostol pretreatment did not significantly affect peptic activity in comparison to the gastric ulcer control group. But it significantly decreased histamine and gastrin contents in comparison to the gastric ulcer control group.
Also, Althaea officinalis pretreatment significantly decreased peptic activity, histamine, and gastrin contents after gastric-ulceration induced by pyloric-ligation/indomethacin administration.
Data of the current work demonstrated that Solanum nigrum extract significantly decreased histamine content. Similar results were obtained demonstrating that the extract offers anti-ulcer activity by blocking acid secretion through inhibition of H + /K + -ATPase [1,24]. Furthermore, Solanum nigrum significantly decreased gastrin content. Similarly, results of previous studies have reported that the gastro-protective effect of Solanum nigrum caused suppression of gastrin release thereby decreasing the acidity of the gastric juice [1,24].
Although the inhibition of cyclooxygenase and deficiency of endogenous prostaglandin is accepted as a main mechanism implicated in indomethacin-induced gastric-ulceration, there is much evidence suggesting the involvement of oxidative stress in this pathology [69,106]. Indomethacin is recognized as an inducer of ROS in animal models, leading to mucosal injury [69]. The role of toxic oxygen free radicals in the genesis of indomethacin-induced gastric ulcers has been shown [106]. Organisms have enzymatic and non-enzymatic defense mechanisms against the toxicity and tissue damage of ROS [69]. Preventive anti-oxidants, such as SOD and GSH, can decrease the gastric mucosal damaging effect of NSAIDs [5,6,107,108]. Lipid peroxidation is the result of ROS interaction with the cell membrane, subsequently generating MDA to cause oxidative gastric damage [19]. Agents such as indomethacin initiate lipid peroxidation by functioning as oxidants and cause damage by producing ROS [109]. Indomethacin significantly increased the lipid peroxidation (MDA) and decreased the level of non-enzymatic (GSH) and the activity of enzymatic (SOD) anti-oxidants in the gastric mucosa of an indomethacin-administered rat [8,91].
Indomethacin was found to produce ulcers via increasing gastric acid secretion and decreasing NO synthesis [75,110]. NO is an endogenous defensive factor for gastric cells and exhibits gastro-protective properties against different types of aggressive agents [18]. NO is involved in the maintenance of mucosal integrity through the regulation of mucus and alkaline secretions, gastric motility and microcirculation [111], modulate acid levels and blood flow in gastric tissues, modulation of mast cell activity [112], prevent membrane lipid peroxidation [8], and promotion of PG-E 2 synthesis [18,20,89]. Evidence exists that escalating the levels of both PG-E 2 and NO boosts gastric mucus formation and secretion [98].
Previous studies demonstrated an increase in MDA with a decrease in anti-oxidants such as GSH and SOD in gastric mucosa of indomethacin-treated rats [6,18]. Consistent with these findings, this work revealed that administration of indomethacin significantly increased the lipid peroxidation (MDA) and decreased the level of non-enzymatic (GSH) and the activity of enzymatic (SOD) anti-oxidants in the gastric mucosa of an indomethacin-administered rat [91]. The decreased concentration of GSH is typically associated with increased lipid peroxidation, evident in the present study as increased MDA level. This was in agreement with a previous study [8,18] that showed that indomethacin-induced increases in mucosal MDA levels. According to our results, pyloric-ligation/indomethacin administration significantly decreased NO content. Similarly, previous studies obtained the same result [18,98].
Pretreatment with omeprazole significantly increased gastric GSH and NO contents after ulceration, in harmony with previous investigations, whereas omeprazole appears to replenish the GSH levels [100]. Meanwhile, in the present study, omeprazole significantly decreased MDA content and significantly increased gastric SOD activity in comparison to the gastric ulcer control group. This was in agreement with a previous study that showed that indomethacin-induced increases in mucosal MDA levels have been improved by classic anti-ulcer drugs like omeprazole [8]. Additionally, omeprazole significantly attenuated the increase of MDA, and protected the stomach from depletion of GSH in comparison to the ulcer control group [20]. The levels of SOD and CAT were significantly increased at the same time that levels of MDA were significantly decreased in pre-treated groups with omeprazole [113]. As a member of PPIs, lansoprazole has shown an anti-oxidative activity by preventing NSAID-induced increases of gastric mucosal concentrations of MPO (myeloperoxidase) and MDA and depletion of GSH concentration in animals [21].
In addition, misoprostol pretreatment significantly increased gastric NO content after pyloric-ligation/indomethacin administration, in harmony with previous investigations reporting that for the misoprostol-treated group after indomethacin-induced gastric-ulceration, NO synthesis was significantly increased [82]. According to our work results, misoprostol significantly increased gastric GSH content and gastric SOD activity in comparison to the gastric ulcer control group, while it significantly decreased MDA content in comparison to the gastric ulcer control group. In a previous investigation, it was reported that as the cytoprotective effect of misoprostol is well documented, it is not yet known if this effect involves anti-oxidant potential [103]. However, treatments by misoprostol before indomethacin significantly reduced blood and tissue MDA levels. Whether misoprostol has a direct anti-oxidant activity or the reduction in MDA results indirectly from minimizing the stressful conditions imposed by indomethacin, resulting from inhibition of indomethacin-induced gastric ulcers, needs to be clarified [95].
Althaea officinalis pretreatment significantly increased NO, similar results that revealed that flavonoids present in Althaea officinalis are anti-inflammatory and useful in maintaining healthy circulation and are protective against gastric ulcers [96]. Additionally, Althaea officinalis pretreatment significantly increased GSH content and SOD activity after pyloric-ligation/indomethacin administration. Althaea officinalis was reported to promote anti-oxidant activity in a neotetrazolium model of injury [60]. Furthermore, Althaea officinalis pretreatment significantly reduced ulcers associated with oxidative stress as manifested by a significant decrease in MDA in comparison to the gastric ulcer control group. Tannin content in Althaea officinalis (scopoletin) mimics hepatic lipid peroxidation and promotes anti-oxidants activity of superoxide-dismutase and catalase [68]. Also, it was demonstrated that flavonoids present in Althaea officinalis can scavenge free radicals and can inhibit lipid peroxidation [32].
Data of the current work also demonstrated that Solanum nigrum extract significantly increased gastric GSH and NO contents and SOD activity but significantly decreased MDA content after pyloric-ligation/indomethacin administration. These results are in harmony with those reporting that the decreased levels of anti-oxidant enzymes and increased mucosal injury were altered to near normal status upon pretreatment with Solanum nigrum when compared to the ulcer induced rats. So, Solanum nigrum may exert its gastro-protective (cytoprotective) effect by a free radical scavenging activity and inhibition of lipid peroxidation, thus suggesting its probable mechanism of action. Activities of GSH, lipid peroxides (LPO), and SOD were determined to show that Solanum nigrum pretreated rats maintained the protein level, anti-oxidant enzyme status, and LPO levels at near normalcy when compared to ulcerated animals. The extract increases the level of reduced GSH and decreases lipid peroxidation [5,61].
Prostaglandins (PG-E 2 and PG-I 2 ) are believed to expose gastro-protective effects by decreasing stomach acid secretion, stimulating the synthesis and secretion of mucus and bicarbonate, increasing the thickness of mucus layers, and improving the blood flow of mucosa [8,114] and the maintenance of cellular integrity in the gastric mucosa [115]. PG-E 2 and PG-I 2 are potent vasodilators and are involved in the maintenance of homeostasis and gastric ulcer healing [12]. Previous studies reported that PG-E 2 acts as a potential inhibitor of TNF-α [116]. PG-I 2 is known to increase the mucus production in superficial epithelial cells, which get inhibited with the use of NSAIDs like indomethacin [117].
Indomethacin administration to shay rats showed a significant decrease in PG-E 2 content in comparison to normal control rats. Suleyman et al. and Inas et al. reported that indomethacin causes gastric damage by reducing PG-E 2 levels in stomach tissue [8,18]. In addition, our results showed a significant decrease in PG-I 2 content in ulcerated rats in comparison to normal control rats. This result is in harmony with the results of previous studies [12,117].
Pretreatment with omeprazole significantly increased PG-E 2 content in comparison to the gastric ulcer control group. This result is in harmony with that obtained in previous investigations [94]. Pretreatment with omeprazole prevented its reduction in the gastric mucosa in comparison to the ulcer control group [20]. Treatment with omeprazole, orally, can upregulate and increase the mucosal PG-E 2 level [116]. In contrast, pretreatment with omeprazole did not significantly affect PG-I 2 in comparison to the gastric ulcer control group.
Misoprostol pretreatment significantly increased PG-E 2 content in comparison to the gastric ulcer control group. Previous investigations demonstrated that misoprostol indicates a gastro-protective effect probably by a pathway that involves the synthesis of prostaglandins [115]. Additionally, treatment by misoprostol caused a significant reduction in indomethacin-induced gastric-ulceration; such a cytoprotective effect is expected from a prostaglandin pathway [95]. In addition, pretreatment with misoprostol did not significantly change PG-I 2 in comparison to the gastric ulcer control group.
Althaea officinalis pretreatment significantly increased PG-E 2 but did not significantly change PG-I 2 in comparison to the gastric ulcer control group. In contrast, another study demonstrated that the flavonoids present in Althaea officinalis can improve blood circulation [32].
In addition, Solanum nigrum extract pretreatment increased PG-E 2 significantly and PG-I 2 (but not significantly) contents in comparison to gastric ulcer control group. Later, Solanum nigrum was able to produce a significant reduction of the gastric mucosal damage induced by indomethacin. So Jainu and Devi concluded the probable local increase in prostaglandin synthesis [1].
NSAIDs (like indomethacin) might induce the synthesis of pro-inflammatory cytokines (like TNF-α and leukotrienes as IL-1β) [91,107,116]. TNF-α and IL-1β are the key immunoregulatory cytokines that amplify the inflammatory response by activating a cascade of immune cells [1,26]. TNF-α overproduction increases the risk of gastric ulcer and cancer [18]. TNF-α also provokes its pro-inflammatory activity via increased IL-1β production, initiation of cytotoxic, apoptotic responses, leukocyte activation, and tissue infiltration [26,118].
According to our results, pyloric-ligation/indomethacin administration (25 mg/kg, i.p.) showed significantly elevated gastric TNF-α and IL-1β contents. These results are in accordance with the work of other investigators [91] and also in harmony with the results obtained by Inas et al. who demonstrated a significant increase in serum TNF-α content in indomethacin administrated shay rats [18].
Pretreatment with omeprazole significantly decreased TNF-α and IL-1β contents in comparison to the gastric ulcer control group, in accordance with studies which concluded that pretreatment with omeprazole significantly inhibited the increase in pro-inflammatory cytokine in comparison to the ulcer control group [20]. Also, in groups pre-treated with omeprazole, it was observed that there was significant reduction in the contents of TNF-α and IL-6 and at the same time significant elevation in the content of IL-10 in comparison to the indomethacin-administered rat group [113].
Misoprostol pretreatment significantly decreased TNF-α and IL-1β contents in comparison to the gastric ulcer control group.
Althaea officinalis pretreatment significantly decreased TNF-α and IL-1β contents in comparison to the gastric ulcer control group. Significant anti-inflammatory (acute and chronic inflammation) and anti-ulcerogenic activities were observed after administration of aqueous extract of Althaea officinalis flower [25]. Also, the flavonoids present in Althaea officinalis have anti-inflammatory and antitumor agents, moreover, some of them have antiproliferative and apoptotic effects on cancer cell lines [32].
Solanum nigrum pretreatment significantly decreased TNF-α and IL-1β contents in comparison to the gastric ulcer control group. Solanum nigrum treatment decreased the level of blood serum TNF-α, and this corresponds to triggering of apoptosis in tumor cells [88].
Heme-oxygenase-1 (HO-1) is considered to be a cytoprotective enzyme [91]. HO-1 and its metabolites have the potential to counteract the NSAIDs-induced gastric injury and show its gastro-protective effects by different mechanisms, including anti-oxidant and anti-inflammatory properties and their ability to restore mucosal blood flow [91,119].
Gaseous vasoactive mediators, such as hydrogen sulfide (H 2 S), were shown to play an important role in the mechanism of mucosal defense and gastro-protection [120,121]. H 2 S is biosynthesized by the activity of two enzymes: cystathionine-γ-lyase (CSE) and cystathionine-β-synthase (CBS) [120][121][122][123][124]. Gastric mucosa expresses both CSE and CBS, which have the ability to mediate H 2 S synthesis [121,124,125]. It has been demonstrated that H 2 S protects the GI tract against gastric damage induced by various factors [120]. H 2 S exerts a gastro-protection effect through decreased production of pro-inflammatory cytokines (IL-1β and TNF-α), reduction of gastric acid secretion (decrease acid output) along with pepsin activity, increase of endogenous prostaglandins production, regulated gastric mucosal blood flow, stimulation of bicarbonate secretion, increase of gastric juice pH and mucin concentration, decrease of reactive oxygen metabolite production, and enhancement of tissue repair by increase of GSH, CAT, and SOD enzymes activities and decrease of lipid peroxidation products [120,121,123]. In the gastrointestinal tract (GI), recent studies suggest that H 2 S may contribute to mucosal defense against injury caused by non-steroidal anti-inflammatory drugs [125]. Inhibition of H 2 S generation is a new, COX independent, mechanism of action of NSAIDs [124].
In the present work, indomethacin administration to shay rats significantly reduced the gastric mucosal HO-1 in spite of considering indomethacin as one of the cellular stress inducers. These results are in harmony with the results obtained by previous works of Allam and El-Gohary who observed that indomethacin administration reduced gastric HO-1 level significantly in comparison to the normal control group [91]. In addition, exposing rats to pyloric-ligation/indomethacin administration showed a significant decrease in CBS activity in comparison to the normal control group, in harmony with other investigations that highlighted the contribution of H 2 S to gastric mucosal defense, as well as the ability of NSAIDs to suppress endogenous H 2 S synthesis through reducing the activity and expression of CSE [122,123].
Pretreatment with omeprazole and misoprostol did not significantly affect CBS activity. But it significantly increased HO-1 activity in comparison to the gastric ulcer control group.
On other hand, our results revealed that Althaea officinalis and Solanum nigrum pretreatments significantly increased CBS and HO-1 activities in comparison to the gastric ulcer control group.

Conclusions
The present study demonstrates the in vitro and in vivo anti-oxidant potential of Althaea officinalis and Solanum nigrum extracts. In addition, it was demonstrated that oral administration of Althaea officinalis and Solanum nigrum extracts, once daily for 14 days, can protect against pyloric-ligation/indomethacin-induced gastric-ulceration in rats, probably via their anti-oxidant properties, inhibition of histamine and gastrin release, suppression of pro-inflammatory cytokines formation like TNF-α and IL-1β, and promotion of mucin, NO, PG-E 2 , and PG-I 2 contents. Also, both extracts can increase the expressions of the protective HO-1 and CBS enzymes in gastric mucosal tissue, which is promising for further clinical trials.