Astragalin from Thesium chinense: A Novel Anti-Aging and Antioxidant Agent Targeting IGFR/CD38/Sirtuins

Astragalin (AG), a typical flavonoid found in Thesium chinense Turcz (T. chinense), is abundant in various edible plants and possesses high nutritional value, as well as antioxidant and antibacterial effects. In this study, we initially predicted the mechanism of action of AG with two anti-aging and antioxidant-related protein targets (CD38 and IGFR) by molecular docking and molecular dynamics simulation techniques. Subsequently, we examined the anti-aging effects of AG in Caenorhabditis elegans (C. elegans), the antioxidant effects in zebrafish, and verified the related molecular mechanisms. In C. elegans, AG synergistically extended the lifespan of C. elegans by up-regulating the expression of daf-16 through inhibiting the expression of daf-2/IGFR and also activating the AMPK and MAPK pathways to up-regulate the expression of sir-2.1, sir-2.4, and skn-1. In oxidatively damaged zebrafish embryos, AG demonstrated a synergistic effect in augmenting the resistance of zebrafish embryos to oxidative stress by up-regulating the expression levels of SIRT1 and SIRT6 within the zebrafish embryos system via the suppression of CD38 enzymatic activity and then inhibiting the expression of IGFR through high levels of SIRT6. These findings highlight the antioxidant and anti-aging properties of AG and indicate its potential application as a supplementary ingredient in aquaculture for enhancing fish health and growth.

C. elegans has been extensively utilized globally as a preferred model organism for the evaluation of bioactive compounds that exhibit effects on longevity [12,13] and has revealed numerous breakthrough discoveries in the field of antioxidant research [14].
In addition, zebrafish embryos, a convenient, reliable, and inexpensive animal model for statistically dose-dependent toxicological studies [15], have been commonly used to screen plant bioactive constituents for antioxidant activity [16].Based on the high conservation of daf-2/IGFR between C. elegans and mammals [17], researchers found that biologically active compounds with inhibitory effects on IGFR could reduce IIS signaling in C. elegans and thus up-regulate the transcription factor daf-16/FOXO [18].For example, Oleuropein enhances stress resistance and extends lifespan by inhibiting the expression of daf-2/IGFR in C. elegans [19].
The concept of hormesis is of great importance in the application of nutritional antioxidants.Hormesis action refers to a biphasic dose response in which a low dose of a harmful substance or stress can actually induce an adaptive response in an organism that enhances its resilience and self-protection.For nutritional antioxidants, this means that small doses of antioxidant components can activate endogenous cellular defense mechanisms, such as up-regulating the expression of antioxidant enzymes and repair enzymes, thereby enhancing the antioxidant and repair capacity of cells [20].Natural antioxidants in plants are categorized into three main groups: phenolic compounds, carotenoids, and vitamins.Phenolic compounds are structurally diverse, ranging from simple molecules (e.g., gallic, vanillic, ferulic, and caffeic acids) to polyphenols (e.g., flavonoids and tannins) [21].For example, curcumin is a natural phenolic antioxidant extracted from rhizomes such as turmeric in the ginger family, and studies have shown that curcumin increases the expression of antioxidant enzymes against reactive oxygen species (ROS) by up-regulating AR expression through Nrf2 in a PI3K/Akt-dependent manner [22,23].Mammalian Nrf2/CNC protein is recognized as an antioxidant regulator, and its immediate homologue in C. elegans is skn-1 [24].T. chinense is rich in polyphenols, polysaccharides, alkaloids, volatile oils, amino acids, and other active ingredients, of which the main active ingredient AG is a typical polyphenolic compound.It is hypothesized that AG may also exert anti-aging and antioxidant effects through the skn-1 target, and a preliminary study of its hormesis dose effects has been conducted.
Sirtuins, which participate in NAD+-dependent deacetylation processes [25], are widely regarded as defensive anti-aging components due to the established connection between heightened sirtuin function and prolonged lifespan, as well as reduced function and the onset of age-related ailments [26].Researchers produced a panel of isogenic human stem cell lines with SIRT1-SIRT7 knockouts and found that any sirtuin deficiency leads to accelerated cellular senescence [27].Resveratrol enhances the longevity of various model organisms through the modulation of oxidative stress, energy metabolism, nutrient perception, and epigenetic mechanisms [28].This effect is predominantly achieved through the activation of sirtuin 1, a process that may be compromised in cases of NAD+ insufficiency [29].
Researchers have shown that SIRT6 overexpression leads to a reduction in frailty and lifespan extension in both male and female B6 mice [30].For example, celastrol and melatonin modify SIRT1, SIRT6, and SIRT7 gene expression and improve the response of human granulosa-lutein cells to oxidative stress [31].It is noteworthy that the overexpression of SIRT6 increased IGF-1-binding proteins and altered the phosphorylation levels of IGF-1 signaling components, thereby inhibiting the IGF-1 pathway and prolonging the lifespan of model animals [32].Senescent cells promote tissue NAD+ decline during aging via the activation of CD38 macrophages [33].Reproductively young mice lacking CD38 exhibited larger primordial follicle pools, elevated ovarian NAD+ levels, and increased fecundity relative to wild-type controls.Mammalian female reproductive lifespan is typically significantly shorter than life expectancy and is associated with a decrease in ovarian NAD+ levels [34].
However, few studies have investigated the mechanism of action of AG in delaying aging and its role in fish culture.This research aimed to investigate the mechanism of action of AG in delaying aging and its role in fish culture.The study is generally classified into two parts: prediction and validation.Firstly, we predicted the interactions between AG and IGFR/CD38 using blind docking and molecular dynamics simulations.Then, we explored the lifespan extension effect of AG in C. elegans and the antioxidant effect of AG in the embryonic zebrafish, respectively, and verified the predicted targets by quantitative real-time PCR.

Chemicals and Reagents
LC-MS-grade acetonitrile (ACN) was purchased from Fisher Scientific (Fisher Scientific, Loughborough, Leicestershire, UK).Formic acid was obtained from Tokyo Chemical Industry (TCI-SCT Shanghai, China).Ammonium formate was obtained from Sigma-Aldrich (JT Baker, Phillipsburg, NY, USA).Ultrapure water was generated using a Milli-Q system (Millipore, Bedford, MA, USA).AG and DMSO were purchased from Aladdin (Aladdin, Shanghai, China).An ROS kit was bought from Nanjing Jiancheng Bioengineering Institute (Jiancheng, Nanjing, China).TRIzol Reagent and SuperScript ® II reverse transcriptase were obtained from Invitrogen (Invitrogen, Eugene, OR, USA).The quantitative real-time PCR (qRT-PCR) system was obtained from Takara Bio Inc. (Takara Bio Inc., Shiga, Japan).Thirty percent H 2 O 2 was purchased from Nanjing Chemical Reagent Factory (NJ-Reagent, Nanjing, China).Other reagents and solvents were of analytical grade.

T. chinense Extract
T. chinense was cultivated on the campus of Nanjing Agricultural University (NJAU), harvested on 30 May 2023, and dried in an oven at 60 • C, No. BRC20230530, and stored at room temperature in the College of Life Sciences, Nanjing Agricultural University, B1022.The herbs harvested were identified by Prof. Zengxu Xiang of the College of Horticulture, Nanjing Agricultural University, as the dried whole herb of T. chinense in the Sandalwood family.T. chinense was soaked in ethanol (85%, 1:10 w/v) at room temperature for 24 h and then ultrasonically extracted at 60 • C for 2 h.The filtrate was combined, filtered, and evaporated with a rotary evaporator.The extract was named W.A portion of W (100 g) was suspended in 100 mL water and then extracted (1:1 v/v, 30 min) with ethyl acetate.The organic phases were evaporated and freeze-dried to obtain the extract named EA.

LC-MS/MS Analysis of Extract
LC-MS analysis was performed using a Vanquish UHPLC System (Thermo Fisher Scientific, Waltham, MA, USA) and Q Exctive focus (Thermo Fisher Scientific, Waltham, MA, USA) with ESI ion source.LC-MS method referred to Zelena and Want for specific conditions, placed in the Supplementary Materials [35,36].

Molecular Docking
The X-ray crystal structures of CD38 (PDB: 3DZK) and IGFR (PDB: 5FXS) were obtained from the Protein Data Bank.The protonation state of AG was set at pH = 7.4, and AG was expanded to a 3D structure using Open Babel [37].AutoDock Tools (ADT3) were applied to prepare and parametrize the receptor protein and ligands.The docking grid documents were generated by AutoGrid of sitemap, and AutoDock Vina (1.2.0) was used for the docking simulation [38,39].The optimal pose was selected to analysis interaction.Finally, the protein-ligand interaction figure was generated by PyMOL (2.5.0,Schrödinger, New York, NY, USA).

Molecular Docking Simulations
The molecular dynamics simulations were carried out with Desmond/Maestro noncommercial version 2022.1 as a molecular dynamic's software [40].TIP3P water molecules were added to the systems, which were then neutralized by 0.15 M NaCl solution.After minimization and relaxation of the system, the production simulation was performed for 100 ns in an isothermal-isobaric ensemble at 300 K and 1 bar.Trajectory coordinates were recorded every 100 ps.The molecular dynamics analysis was performed using Simulation Interaction Diagram from Desmond.

C. elegans Strains and Maintenance
Wild-type Bristol N2, transgenic nematode strains: CF1553 sod-3 p:: GFP (muls84), and Escherichia coli OP50 (E. coli OP50) were obtained from Caenorhabditis Genetics Center (CGC).All nematodes were maintained on a nematode growth medium (NGM) plate with a layer of E. coli OP50 as food at 20 • C. To attain synchronization, C. elegans in the oviposition phase were relocated onto NGM plates devoid of E. coli OP50 bacteria for egg deposition overnight, following which they were subsequently extracted.L1-stage C. elegans hatched from the eggs were then transferred to new NGM plates until they reached the adult stage.AG was dissolved in DMSO and poured onto an NGM plate when the medium was 50 • C during the liquid stage (the final concentration of DMSO was less than 0.1%).

C. elegans Lifespan Assay
Synchronized L1 larvae were cultured on NGM containing 0, 50, 250, and 500 µg/mL of AG in E. coli OP50.C. elegans was transferred to the fresh NGM plates every day, and then all the alive, escaped, and dead data were recorded.C. elegans were scored as dead when they did not respond to repeated touching with a platinum wire.

ROS Level Assay in C. elegans
Synchronized L1 C. elegans were put on an NGM plate containing AG or not at 20 • C for 48 h.Then, the C. elegans were collected and washed 5 times to remove E. coli OP50.Stain ROS in C. elegans using the ROS kit according to the manufacturer's instructions.The fluorescence intensity of ROS was read at an excitation wavelength of 488 nm and an emission wavelength of 525 nm.In addition, the C. elegans were placed on glass slides, and the levels of ROS were observed under a fluorescence microscope (OLYMPUS BX53, Beijing, China).

C. elegans Gene Expression Assay
The synchronized L4 larvae were incubated with or without AG at 20 • C, as described in the lifespan assay.After a 2-day incubation, adult C. elegans were collected for extracting total RNA according to the manufacturer's protocol.Afterwards, cDNA was synthesized using reverse transcriptase (SuperScript ® II).Following this, real-time PCR was completed using the iQ TM SYBR R Green Supermix kit (Bio-Rad, Shanghai, China), and anti-proliferative factor expression was detected by the BIO-RADCFX48 TM real-time system (Bio-Rad, Shanghai, China).Lastly, the relative expression of the anti-proliferative genes was calculated based on the 2 _∆∆Ct .Real-time PCR primer sequences can be found in the Supplementary Materials (Table S1).

Waterborne Exposure of Embryos from Zebrafish
Embryos from zebrafish (AB wild-type) were provided by YSY Biotech (Nanjing, China).After 7-9 h post-fertilization (hpf), embryos were pre-exposed to a medium containing AG (0, 20, 50, and 100 µg/mL) for 2 h.In order to prevent the reaction of AG with H 2 O 2 , the fish culture water containing AG was therefore removed after 2 h of treatment and replaced with fish culture water containing 0.22 mmol/L H 2 O 2 to induce oxidative stress injury.The mounded embryos were incubated in a constant temperature incubator at 28 • C for 96 h.Embryonic mortality and malformation rates were counted.

Assay of ROS Level and Cell Death in Zebrafish Embryos
Samples treated by method Section 2.10.were stained with DCFH-DA and acridine orange and incubated for the appropriate reaction time for each reagent.Embryos were then washed, anesthetized, and observed under a fluorescence microscope.Quantification of fluorescence staining intensity was performed and visualized by utilizing ImageJ 1.54 h software (NIH, Bethesda, MD, USA).

Zebrafish Embryos Gene Expression Assay
Zebrafish embryos were prepared as described in Section 2.10.The expression of antioxidant-related genes in zebrafish embryos was assayed according to Section 2.9.Realtime PCR primer sequences can be found in Supplementary Materials (Table S1).

Statistical Analyses
Origin 2021 software was used for visualization of the data, presented as mean ± standard deviation (SD).The one-way analysis of variance (ANOVA) was performed using SPSS 20.0 software (IBM, Armonk, NY, USA) to analyze differences between groups.Different letters within the same group of treatments indicate a significant difference (p < 0.05).Graphical abstract was conducted by Figdraw (https://www.figdraw.com/,accessed on 15 July 2024).time PCR primer sequences can be found in Supplementary Materials (Table S1).

Statistical Analyses
Origin 2021 software was used for visualization of the data, presented as mean ± standard deviation (SD).The one-way analysis of variance (ANOVA) was performed using SPSS 20.0 software (IBM, Armonk, NY, USA) to analyze differences between groups.Different letters within the same group of treatments indicate a significant difference (p < 0.05).Graphical abstract was conducted by Figdraw (https://www.figdraw.com/,accessed on 15 July 2024).

Molecular Docking of IGFR/CD38 with AG
The IGFR and CD38 proteins are represented as a slate cartoon model, the ligand is shown as a cyan stick, and their binding sites are shown as magenta stick structures.The hydrogen bond, ionic interactions, and hydrophobic interactions are depicted as yellow, magenta, and green dashed lines, respectively.
We analyzed the interactions between the protein and ligand, and all functional residues were identified and classified based on their interactions.There are multiple groups of residues used to form interactions between receptor protein and ligand, such as the hydrogen bond formed by (GLU1080, MET1082, THR1083, SER1089, ASP1153, MET1156) of IGFR-AG and (TRP125, ASP156, SER186, PHE222, GLU226) of CD38-AG (Figure 2).With these interaction forces, the binding energy of IGFR and CD38-AG

Molecular Docking of IGFR/CD38 with AG
The IGFR and CD38 proteins are represented as a slate cartoon model, the ligand is shown as a cyan stick, and their binding sites are shown as magenta stick structures.The hydrogen bond, ionic interactions, and hydrophobic interactions are depicted as yellow, magenta, and green dashed lines, respectively.
We analyzed the interactions between the protein and ligand, and all functional residues were identified and classified based on their interactions.There are multiple groups of residues used to form interactions between receptor protein and ligand, such as the hydrogen bond formed by (GLU1080, MET1082, THR1083, SER1089, ASP1153, MET1156) of IGFR-AG and (TRP125, ASP156, SER186, PHE222, GLU226) of CD38-AG (Figure 2).With these interaction forces, the binding energy of IGFR and CD38-AG complex was all −8.5 kcal/mol, which demonstrates excellent performance.However, the above results are all theoretical calculations and for reference only, everything is subject to experiment.complex was all −8.5 kcal/mol, which demonstrates excellent performance.However, the above results are all theoretical calculations and for reference only, everything is subject to experiment.

Molecular Dynamics Simulations of IGFR/CD38 with AG
The Root Mean Square Deviation (RMSD) value serves as an indicator of alterations in the stability of complex conformation.During the simulation, the RMSD of AG-IGFR and free IGFR has been in a relatively stable state and eventually stabilized between 3.0 and 4.5 Å.The RMSD of AG-CD38 and free CD38 has also been in a relatively stable state and finally stabilized between 2.5 and 4.5 Å.The results showed that AG binding to protein did not cause large fluctuations (Figures 3a and 4a).Moreover, the RMSF value of flexibility of expressed amino acid residues indicated that complexes and free IGFR and CD38 amino acid fluctuations were relatively stable (Figures 3c and 4c).

Molecular Dynamics Simulations of IGFR/CD38 with AG
The Root Mean Square Deviation (RMSD) value serves as an indicator of alterations in the stability of complex conformation.During the simulation, the RMSD of AG-IGFR and free IGFR has been in a relatively stable state and eventually stabilized between 3.0 and 4.5 Å.The RMSD of AG-CD38 and free CD38 has also been in a relatively stable state and finally stabilized between 2.5 and 4.5 Å.The results showed that AG binding to protein did not cause large fluctuations (Figures 3a and 4a).Moreover, the RMSF value of flexibility of expressed amino acid residues indicated that complexes and free IGFR and CD38 amino acid fluctuations were relatively stable (Figures 3c and 4c).
The main interactions of the IGFR along the trajectory were as follows: ionic interactions-GLU1080, ASP1153, ASP1086; hydrophobic interactions-MET1082, Val1013, Ile1160.It is worth mentioning that the hydrogen bond formation frequency of ASP1153 is 99%, suggesting that the ASP1153 amino acid plays a crucial role in the binding process.In addition, the ligand also formed two intramolecular hydrogen bonds for stabilizing the binding conformation of the small molecule (Figure 3b).
The main interactions of CD38 along the trajectory were as follows: hydrophobic interactions-TRP159, Val185, Pro174, TRP189; polar interactions-Asn183, SER186.It is noteworthy that the frequency of hydrogen bond formation for Pro174 is 81%, suggesting that the Pro174 amino acid plays a crucial role in the binding process.Additionally, the ligand also forms an intramolecular hydrogen bond to stabilize the binding conformation of the small molecule (Figure 4b) These results demonstrate the high stability of the IGFR and CD38 and AG complexes, and we could infer that AG could be depressants of the enzyme.

AG Extended the Lifespan of C. elegans
In C. elegans, inhibiting the insulin/IGF-1 pathway through daf-2/IGFR inactivation proved to be a successful method of increasing lifespan.Our investigation focused on the longevity extension effect of caffeoylquinic acids in C. elegans, considering their high binding ability to the inhibitory regions of IGFR.The survival curves of all the nematodes were almost identical until day 11 (Figure 5c,d).
noteworthy that the frequency of hydrogen bond formation for Pro174 is 81%, suggesting that the Pro174 amino acid plays a crucial role in the binding process.Additionally, the ligand also forms an intramolecular hydrogen bond to stabilize the binding conformation of the small molecule (Figure 4b) These results demonstrate the high stability of the IGFR and CD38 and AG complexes, and we could infer that AG could be depressants of the enzyme.

AG Extended the Lifespan of C. elegans
In C. elegans, inhibiting the insulin/IGF-1 pathway through daf-2/IGFR inactivation proved to be a successful method of increasing lifespan.Our investigation focused on the longevity extension effect of caffeoylquinic acids in C. elegans, considering their high binding ability to the inhibitory regions of IGFR.The survival curves of all the nematodes were almost identical until day 11 (Figure 5c,d).However, after day 12, the survival curves of the AG treatment groups shifted to the right relative to the control group, with the 500 µg/mL group showing the most noticeable shift.The average lifespan of the control nematodes was 15 days.After treatment with AG However, after day 12, the survival curves of the AG treatment groups shifted to the right relative to the control group, with the 500 µg/mL group showing the most noticeable shift.The average lifespan of the control nematodes was 15 days.After treatment with AG (50, 250, and 500 µg/mL), the mean longevity of the nematodes was recorded as 18, 17, and 19 days, respectively, demonstrating an increment of 23.13%, 13.13%, and 26.94% compared to the control cohort (Figure 5a,b).

AG Reduces ROS Levels of C. elegans
The control group had the highest levels of ROS, while treatment of AG (50, 250, and 500 µg/mL) decreased the relative ROS levels by 35.68%, 39.97%, and 55.38%, respectively.It is therefore hypothesized that AG may extend the lifespan of C. elegans by reducing the levels of ROS (Figure 6e).

AG Reduces ROS Levels of C. elegans
The control group had the highest levels of ROS, while treatment of AG (50, 250, and 500 µg/mL) decreased the relative ROS levels by 35.68%, 39.97%, and 55.38%, respectively.It is therefore hypothesized that AG may extend the lifespan of C. elegans by reducing the levels of ROS (Figure 6e).

AG Increases SOD Levels of C. elegans
The C. elegans treated with 500 µg/mL AG had the highest SOD levels, which were significantly higher than those of the control and other treatment groups (p < 0.05).However, 50 µg/mL AG had almost no effect on the levels of SOD in C. elegans (Figure 7).Therefore, we speculate that AG can prolong the lifespan by increasing the SOD activity in C. elegans when it reaches a certain dose.

AG Increases SOD Levels of C. elegans
The C. elegans treated with 500 µg/mL AG had the highest SOD levels, which were significantly higher than those of the control and other treatment groups (p < 0.05).However, 50 µg/mL AG had almost no effect on the levels of SOD in C. elegans (Figure 7).Therefore, we speculate that AG can prolong the lifespan by increasing the SOD activity in C. elegans when it reaches a certain dose.

AG Reduces ROS Levels of C. elegans
The control group had the highest levels of ROS, while treatment of AG (50, 250, and 500 µg/mL) decreased the relative ROS levels by 35.68%, 39.97%, and 55.38%, respectively.It is therefore hypothesized that AG may extend the lifespan of C. elegans by reducing the levels of ROS (Figure 6e).

AG Increases SOD Levels of C. elegans
The C. elegans treated with 500 µg/mL AG had the highest SOD levels, which were significantly higher than those of the control and other treatment groups (p < 0.05).However, 50 µg/mL AG had almost no effect on the levels of SOD in C. elegans (Figure 7).Therefore, we speculate that AG can prolong the lifespan by increasing the SOD activity in C. elegans when it reaches a certain dose.

AG Regulates the AMPK Pathway and Sirtuins-Associated mRNA Expression in C. elegans
Sirtuins are known to be protective anti-aging proteins because an increase in sirtuin activity is associated with increased longevity and a decrease in activity is linked to the development of aging-related diseases.Also, Sirtuins are mediators of calorie restriction and play an important role in ameliorating obesity and age-related metabolic diseases.The AMPK pathway is the upstream signaling that regulates the activity of Sirtuins, to determine whether AG has the potential to be a "Sirtfoods", we chose homologous genes in C. elegans (aak-2, sir-2.1, and sir-2.4) and measured their expression.These results indicated that the expression of aak-2, sir-2.1, and sir-2.4 was significantly increased when the concentration of AG was higher than or equal to 250 µg/mL, but there was no significant effect on the expression of aak-2 and sir-2.1 when the concentration of AG was 50 µg/mL (Figure 8).

AG Regulates the AMPK Pathway and Sirtuins-Associated mRNA Expression in C. elegans
Sirtuins are known to be protective anti-aging proteins because an increase in sirtuin activity is associated with increased longevity and a decrease in activity is linked to the development of aging-related diseases.Also, Sirtuins are mediators of calorie restriction and play an important role in ameliorating obesity and age-related metabolic diseases.The AMPK pathway is the upstream signaling that regulates the activity of Sirtuins, to determine whether AG has the potential to be a "Sirtfoods", we chose homologous genes in C. elegans (aak-2, sir-2.1, and sir-2.4) and measured their expression.These results indicated that the expression of aak-2, sir-2.1, and sir-2.4 was significantly increased when the concentration of AG was higher than or equal to 250 µg/mL, but there was no significant effect on the expression of aak-2 and sir-2.1 when the concentration of AG was 50 µg/mL (Figure 8).

AG Regulates the IIS Pathway and MAPK-Associated mRNA Expression in C. elegans
Numerous studies have confirmed the mechanism of action by which IIS regulates daf-16 to extend lifespan.To investigate that AG mediates lifespan in an IIS pathwaydependent manner, we assessed mRNA expression of transcription factors associated with the IIS pathway.AG down-regulated the expression of daf-2 (p < 0.05), which was consistent with the results of the preliminary molecular docking and molecular dynamics simulations, proving that AG could bind to IGFR/daf-2 intensively and exert an inhibitory effect (Figure 9).
In addition, 500 µg/mL of AG significantly up-regulated the expression of daf-16, skn-1, and sod-3 (p < 0.05), which is consistent with the results of the SOD levels assay.However, AG had almost no effect on sod-5 expression.Notably, the regulation of skn-1 is usually dependent on the MAPK pathway.The examination was conducted on the expression of two crucial transcription factors, sek-1 and tir-1, within the MAPK pathway (Figure 9).The results revealed a significant increase in the expression levels of both factors when the concentration of AG reached or exceeded 250 µg/mL (p < 0.05).

AG Regulates the IIS Pathway and MAPK-Associated mRNA Expression in C. elegans
Numerous studies have confirmed the mechanism of action by which IIS regulates daf-16 to extend lifespan.To investigate that AG mediates lifespan in an IIS pathwaydependent manner, we assessed mRNA expression of transcription factors associated with the IIS pathway.AG down-regulated the expression of daf-2 (p < 0.05), which was consistent with the results of the preliminary molecular docking and molecular dynamics simulations, proving that AG could bind to IGFR/daf-2 intensively and exert an inhibitory effect (Figure 9).

AG Inhibits H2O2-Induced Malformation Rate and Cell Death in Zebrafish Embryos
Oxidative stress leads to malformations such as damage to the egg membrane, abnormal curvature of the spine, and edema of the pericardium in zebrafish embryos.However, after AG treatment, AG (50, 250, and 500 µg/mL) significantly reduced the malformation rate of zebrafish embryos relative to the model group (p < 0.05) (Figure 10a,b).In addition, the rate of cellular mortality in zebrafish embryos exhibited a notable In addition, 500 µg/mL of AG significantly up-regulated the expression of daf-16, skn-1, and sod-3 (p < 0.05), which is consistent with the results of the SOD levels assay.However, AG had almost no effect on sod-5 expression.Notably, the regulation of skn-1 is usually dependent on the MAPK pathway.The examination was conducted on the expression of two crucial transcription factors, sek-1 and tir-1, within the MAPK pathway (Figure 9).The results revealed a significant increase in the expression levels of both factors when the concentration of AG reached or exceeded 250 µg/mL (p < 0.05).

AG Inhibits H 2 O 2 -Induced Malformation Rate and Cell Death in Zebrafish Embryos
Oxidative stress leads to malformations such as damage to the egg membrane, abnormal curvature of the spine, and edema of the pericardium in zebrafish embryos.However, after AG treatment, AG (50, 250, and 500 µg/mL) significantly reduced the malformation rate of zebrafish embryos relative to the model group (p < 0.05) (Figure 10a,b).In addition, the rate of cellular mortality in zebrafish embryos exhibited a notable decrease subsequent to the administration of AG in comparison to the control group (p < 0.05) (Figure 10c-h).

AG Inhibits H2O2-Induced Malformation Rate and Cell Death in Zebrafish Embryos
Oxidative stress leads to malformations such as damage to the egg membrane, abnormal curvature of the spine, and edema of the pericardium in zebrafish embryos.However, after AG treatment, AG (50, 250, and 500 µg/mL) significantly reduced the malformation rate of zebrafish embryos relative to the model group (p < 0.05) (Figure 10a,b).In addition, the rate of cellular mortality in zebrafish embryos exhibited a notable decrease subsequent to the administration of AG in comparison to the control group (p < 0.05) (Figure 10c-h).

AG Reduces ROS Levels of Zebrafish Embryos
Similar to the trend of AG on H 2 O 2 -induced malformation rate in zebrafish embryos, after treatment with AG, the level of ROS within zebrafish embryos was significantly reduced compared to the model group (p < 0.05) (Figure 11).It is hypothesized that AG may exert antioxidant effects by reducing ROS levels in oxidatively damaged zebrafish embryos.

AG Reduces ROS Levels of Zebrafish Embryos
Similar to the trend of AG on H2O2-induced malformation rate in zebrafish embryos, after treatment with AG, the level of ROS within zebrafish embryos was significantly reduced compared to the model group (p < 0.05) (Figure 11).It is hypothesized that AG may exert antioxidant effects by reducing ROS levels in oxidatively damaged zebrafish embryos.

AG Regulates Sirtuins-Associated mRNA Expression in Zebrafish Embryos
CD38 is an NAD+-depleting enzyme that regulates the expression of Sirtuins by affecting changes in NAD+ levels.Since there is no homologous gene for CD38 in C. elegans but there is in zebrafish embryos, we used zebrafish embryos as a model animal to

AG Regulates Sirtuins-Associated mRNA Expression in Zebrafish Embryos
CD38 is an NAD+-depleting enzyme that regulates the expression of Sirtuins by affecting changes in NAD+ levels.Since there is no homologous gene for CD38 in C. elegans but there is in zebrafish embryos, we used zebrafish embryos as a model animal to investigate the role of AG in the regulation of CD38 during oxidative stress (Figure 12a).The outcomes of molecular docking and molecular dynamics simulations revealed a significant binding energy demonstrated by AG towards CD38 and IGFR.This observation aligns with the notable down-regulation of both CD38 and IGFR expressions in the treated cohort compared to the oxidative damage cohort post AG intervention, as evidenced by statistical significance (p < 0.05).Interestingly, after AG treatment, two important members of the Sirtuins gene family (SIRT1 and SIRT6) were significantly up-regulated compared to the oxidative damage group (p < 0.05) (Figure 12b-d).
significant binding energy demonstrated by AG towards CD38 and IGFR.Thi observation aligns with the notable down-regulation of both CD38 and IGFR expression in the treated cohort compared to the oxidative damage cohort post AG intervention, a evidenced by statistical significance (p < 0.05).Interestingly, after AG treatment, two important members of the Sirtuins gene family (SIRT1 and SIRT6) were significantly up regulated compared to the oxidative damage group (p < 0.05) (Figure 12b-d).

Discussion
Increased oxidative stress has been associated with the aging process [41].Oxidativ injury could potentially exacerbate the oxidative load associated with typical aerobi cellular processes, a phenomenon that inherently produces oxidizing agents, leads to th accumulation of oxidative damage within mitochondria, and participates in the natura aging process.The increase in oxidative damage may, in part, contribute to stress associated acceleration of aging [42].Many types of natural compounds, such a flavonoids and vitamins, have been shown to exert antioxidant and anti-aging effects in vivo and in vitro [43].For example, the cellular levels of ROS and antioxidant enzyme were regulated by the main flavonoids of propolis extract, demonstrating both beneficia antioxidant and pro-oxidant effects [44].Inspired by the effects of reversing oxidativ stress through pretreatment with tomato and rosemary extracts [45], we found that AG had a similar effect.AG is a common flavonoid derived from T. chinense, which is also found in a wide range of edible plants (Paeonia lactiflora [46], Artemisia absinthium L. [47] and Rhodomyrtus tomentosa [48]).T. chinense has been receiving increasing attention for it applications and research recently.Therefore, in addition to using molecular docking and

Discussion
Increased oxidative stress has been associated with the aging process [41].Oxidative injury could potentially exacerbate the oxidative load associated with typical aerobic cellular processes, a phenomenon that inherently produces oxidizing agents, leads to the accumulation of oxidative damage within mitochondria, and participates in the natural aging process.The increase in oxidative damage may, in part, contribute to stress-associated acceleration of aging [42].Many types of natural compounds, such as flavonoids and vitamins, have been shown to exert antioxidant and anti-aging effects in vivo and in vitro [43].For example, the cellular levels of ROS and antioxidant enzymes were regulated by the main flavonoids of propolis extract, demonstrating both beneficial antioxidant and pro-oxidant effects [44].Inspired by the effects of reversing oxidative stress through pretreatment with tomato and rosemary extracts [45], we found that AG had a similar effect.AG is a common flavonoid derived from T. chinense, which is also found in a wide range of edible plants (Paeonia lactiflora [46], Artemisia absinthium L. [47], and Rhodomyrtus tomentosa [48]).T. chinense has been receiving increasing attention for its applications and research recently.Therefore, in addition to using molecular docking and molecular dynamics simulation techniques, we also innovatively conducted a study on the anti-aging and antioxidant activities of AG using C. elegans and zebrafish embryos.
ROS plays a role in the aging process of cells and contributes to various physiological signaling pathways [49].According to the free radical theory of aging, aging is a result of the accumulated damage caused by ROS [50].Oxygen-free radicals are believed to be involved in the aging process.Superoxide dismutase (SOD) is crucial for antioxidative defense [51] and is one of the most effective mechanisms in physiology for neutralizing reactive oxygen species [52].The current study revealed that the lifespan and SOD levels were enhanced, and ROS levels were reduced, in C. elegans treated with 500 µg/mL AG.
Molecular docking and molecular dynamics simulation techniques are commonly used to uncover drug-protein interactions [53] and elucidate the mechanism of drug action [54].For instance, two natural depressants of xanthine oxidase were identified through molecular docking and molecular dynamics simulations [55].In this study, molecular docking and molecular dynamics simulations were employed to investigate AG, which binds to IGFR and CD38.IGFR and CD38 are key proteins of oxidative stress.The binding energies of the complexes were all −8.5 kcal/mol, and the RMSD and RMSF values of the complexes remained relatively stable.These results indicate that AG binds securely to IGFR and CD38 proteins during MD simulation.The results were validated through experiments on C. elegans and zebrafish embryos.Consequently, it is suggested that AG may exhibit antioxidant and anti-aging effects by targeting IGFR and modulating CD38 activity.
A study found the insulin/IGF receptor homolog daf-2 regulates aging in C. elegans.Decreasing daf-2 activity causes fertile adults to remain active much longer than normal and to live more than twice as long [56].This could be because reduced daf-2 signaling leads to changes in downstream targets via the daf-16 gene, a fork-head transcription factor, which is regulated by daf-2, resulting in an extended lifespan Another study discovered that Acrolein promotes aging and oxidative stress via the stress response factor daf-16/FOXO in C. elegans [58].In the present study, we observed a reduction in the expression of daf-2 and the expression of daf-16 in C. elegans treated with 500 µg/mL AG.Therefore, we hypothesized that AG might have an anti-aging effect by inhibiting the activity of daf-2/IGFR through binding to daf-2/IGFR, thereby activating daf-16.
Decreased NAD+ levels have been shown to contribute to metabolic dysfunction during aging.NAD+ decline can be partially prevented by knockout of the enzyme CD38 [59].In CD38 knockout mice, tissue levels of NAD+ are significantly increased [60].In d-gal-induced acute aging mice, CD38 and SIRT6 exhibited increased and decreased expression, respectively, in myocardial tissues.This is because CD38 down-regulates SIRT6 expression to promote cell senescence [61].SIRT6, a nuclear histone deacetylase, functions at the chromatin level to directly attenuate IGF signaling.SIRT6-deficient mouse hearts exhibited hyperactivation of IGF signaling-related genes and their downstream targets.Mechanistically, SIRT6 binds to and suppresses the promoter of IGF signaling-related genes by interacting with c-Jun and deacetylating histone 3 at Lys9 (H3K9) [62].Similar to dietary restriction, mice overexpressing the NAD+-dependent protein deacylase SIRT6 (MOSES) live longer and have reduced IGF-1 levels [63].
AMPK activates histone deacetylases (HDACs) and Sirtuins by increasing the cellular concentration of NAD+, a cofactor of Sirtuins [64].The AMPK signaling pathway regulates a comprehensive signaling network that plays a role in governing both healthspan and lifespan, for instance, through the modulation of SIRT1 signaling cascades [65].Moreover, caloric restriction is believed to slow down aging by boosting the activity of some Sirtuins through activating adenosine monophosphate-activated protein kinase (AMPK), thus raising the level of intracellular nicotinamide adenine dinucleotide NAD+ by stimulating NAD+ biosynthesis [66].In the present study, we found that the expression of aak-2, sir-2.1, and sir-2.4 was increased in C. elegans treated with 500 µg/mL AG.Therefore, we hypothesized that AG may play an anti-aging role by activating the AMPK pathway to increase the level of NAD+, consequently activating the expression of the Sirtuins genes.
In C. elegans, the skn-1 gene encodes a transcription factor that resembles mammalian Nrf2 and activates a detoxification response.Skn-1 promotes resistance to oxidative stress (Oxr) and also increases lifespan.It has been suggested that the former causes the latter, consistent with the theory that oxidative damage causes aging [67].Furthermore, the skn-1 transcription factor is considered by some to be an evolutionarily conserved regulator of exogenous stress and longevity [68].Importantly, skn-1 plays a central role in diverse genetic and pharmacologic interventions that promote C. elegans longevity, suggesting that mechanisms regulated by skn-1 may be of conserved importance in aging.These C. elegans studies predict that mammalian Nrf/CNC protein functions and regulation may be similarly complex and that the proteins and processes that they regulate are likely to have a major influence on mammalian life and healthspan [24].Skn-1 is mainly regulated by the upstream signaling pathway MAPK [69].In this study, we found that the expressions of sek-1, tir-1, skn-2, and sod-3 were all elevated in C. elegans treated with 500 µg/mL AG; so, we hypothesized that AG may play an anti-aging role by activating sek-1 and tir-1 in the MAPK pathway, which increases the expression of skn-1 and thus activates its downstream signal, sod-3.
Similarly, in zebrafish embryos, after AG treatment, the levels of SIRT1 and SIRT6 in oxidatively damaged zebrafish embryos were increased and the levels of ROS, IGFR, and CD38 were reduced.Combining the results of molecular docking and molecular dynamics simulation, we speculate that AG can not only directly bind to IGFR to inhibit its activity but also bind to CD38 to inhibit the activity of CD38, increase the level of NAD+, activate the expression of SIRT1 and SIRT6, and inhibit the activity of IGFR by up-regulation of SIRT6, thus synergistically exerting the antioxidant effect (Figure 13).
genetic and pharmacologic interventions that promote C. elegans longevity, suggesting that mechanisms regulated by skn-1 may be of conserved importance in aging.These C. elegans studies predict that mammalian Nrf/CNC protein functions and regulation may be similarly complex and that the proteins and processes that they regulate are likely to have a major influence on mammalian life and healthspan [24].Skn-1 is mainly regulated by the upstream signaling pathway MAPK [69].In this study, we found that the expressions of sek-1, tir-1, skn-2, and sod-3 were all elevated in C. elegans treated with 500 µg/mL AG; so, we hypothesized that AG may play an anti-aging role by activating sek-1 and tir-1 in the MAPK pathway, which increases the expression of skn-1 and thus activates its downstream signal, sod-3.
Similarly, in zebrafish embryos, after AG treatment, the levels of SIRT1 and SIRT6 in oxidatively damaged zebrafish embryos were increased and the levels of ROS, IGFR, and CD38 were reduced.Combining the results of molecular docking and molecular dynamics simulation, we speculate that AG can not only directly bind to IGFR to inhibit its activity but also bind to CD38 to inhibit the activity of CD38, increase the level of NAD+, activate the expression of SIRT1 and SIRT6, and inhibit the activity of IGFR by up-regulation of SIRT6, thus synergistically exerting the antioxidant effect (Figure 13).

Conclusions
This study demonstrated that a typical flavonoid from T. chinense, AG, could increase the expression of daf-16 by inhibiting the expression of IGFR/daf-2, thereby prolonging the lifespan of C. elegans.It is worth mentioning that after the preventive treatment with AG, both IGFR and CD38 were inhibited in oxidatively damaged zebrafish embryos, and ROS content was significantly decreased, which improved the resistance to oxidative stress in zebrafish embryos.In addition, in C. elegans, AG activated transcription factors related to the AMPK/ MAPK/IIS pathway despite the inhibition of IGFR/daf-2, thereby regulating the lifespan of C. elegans.However, the interactions among transcription factors need further in-depth study.In summary, the previous studies further demonstrated the safety of AG and its anti-aging and antioxidant efficacy.These results help to explain the anti-aging effects of AG on C. elegans.The results also support the use of AG as a feed additive to enhance the antioxidant capacity of aquatic products.However, there are some limitations of this study.For instance, C. elegans are deficient in several mammalian metabolic pathways, such as cardiovascular and hepatic first-pass metabolism.Furthermore, we did not utilize C. elegans mutant (e.g., EU1 and CL2166, LG348, LG357) knockout mice to further validate the antioxidant and anti-aging mechanisms of AG; therefore, potential directions for our future research may include the application of animal models (e.g., mice, monkeys) as well as the C. elegans mutant or implementing clinical trials to explore the antioxidant and anti-aging properties of AG.

Figure 1 .
Figure 1.LC-MS/MS analysis of extract: (a) base peak chromatograms (BPCs) of EA of T. chinense and (b) chemical structures of AG.

Figure 1 .
Figure 1.LC-MS/MS analysis of extract: (a) base peak chromatograms (BPCs) of EA of T. chinense and (b) chemical structures of AG.

Figure 5 .
Figure 5. AG extended the lifespan of C. elegans: (a) effect of AG on the mean lifespan of N2 wildtype C. elegans; (b) effect of AG on the life extension rate of N2 wild-type C. elegans; (c) effect of AG on the survival curve of N2 wild-type C. elegans; (c) effect of AG on the max lifespan of N2 wild-type C. elegans; and (d) effect of AG on the survival curve of N2 wild-type C. elegans.The experiment was repeated at least three times.Notes: No common letter in the same treatment group indicates a significant difference (p < 0.05).

Figure 5 .
Figure 5. AG extended the lifespan of C. elegans: (a) effect of AG on the mean lifespan of N2 wild-type C. elegans; (b) effect of AG on the life extension rate of N2 wild-type C. elegans; (c) effect of AG on the survival curve of N2 wild-type C. elegans; (c) effect of AG on the max lifespan of N2 wild-type C. elegans; and (d) effect of AG on the survival curve of N2 wild-type C. elegans.The experiment was repeated at least three times.Notes: No common letter in the same treatment group indicates a significant difference (p < 0.05).

Figure 6 .
Figure 6.AG reduces ROS levels of C. elegans: (a) ROS levels of C. elegans treated without AG; (b) ROS levels of C. elegans treated with 50 µg/mL AG; (c) ROS levels of C. elegans treated with 250 µg/mL AG; (d) ROS levels of C. elegans treated with 500 µg/mL AG; and (e) effect of AG on the ROS of C. elegans.The experiment was repeated at least three times.In the absence of a common letter within the same treatment group, this indicates a significant difference (p < 0.05).

Figure 6 .
Figure 6.AG reduces ROS levels of C. elegans: (a) ROS levels of C. elegans treated without AG; (b) ROS levels of C. elegans treated with 50 µg/mL AG; (c) ROS levels of C. elegans treated with 250 µg/mL AG; (d) ROS levels of C. elegans treated with 500 µg/mL AG; and (e) effect of AG on the ROS of C. elegans.The experiment was repeated at least three times.In the absence of a common letter within the same treatment group, this indicates a significant difference (p < 0.05).

Figure 6 .
Figure 6.AG reduces ROS levels of C. elegans: (a) ROS levels of C. elegans treated without AG; (b) ROS levels of C. elegans treated with 50 µg/mL AG; (c) ROS levels of C. elegans treated with 250 µg/mL AG; (d) ROS levels of C. elegans treated with 500 µg/mL AG; and (e) effect of AG on the ROS of C. elegans.The experiment was repeated at least three times.In the absence of a common letter within the same treatment group, this indicates a significant difference (p < 0.05).

Figure 8 .
Figure 8. AG regulates the AMPK pathway and Sirtuins-associated mRNA expression in C. elegans: (a) effect of AG on aak-2 expression in C. elegans; (b) effect of AG on sir-2.1 expression in C. elegans; and (c) effect of AG on sir-2.4expression in C. elegans.The experiment was repeated at least three times.Note: No common letter in the same treatment group indicates a significant difference (p < 0.05).

Figure 8 .
Figure 8. AG regulates the AMPK pathway and Sirtuins-associated mRNA expression in C. elegans: (a) effect of AG on aak-2 expression in C. elegans; (b) effect of AG on sir-2.1 expression in C. elegans; and (c) effect of AG on sir-2.4expression in C. elegans.The experiment was repeated at least three times.Note: No common letter in the same treatment group indicates a significant difference (p < 0.05).

Figure 9 .
Figure 9. AG regulates the IIS pathway and AMPK-associated mRNA expression in C. elegans: (a) effect of AG on skn-1 expressions in C. elegans; (b) effect of AG on daf-2 expressions in C. elegans; (c) effect of AG on daf-16 expressions in C. elegans; (d) effect of AG on sek-1 expressions in C. elegans; (e) effect of AG on sod-3 expressions in C. elegans; (f) effect of AG on sod-5 expressions in C. elegans; and (g) effect of AG on tir-1 expressions in C. elegans.The experiment was repeated at least three times.Note: No common letter in the same treatment group indicates a significant difference (p < 0.05).

Figure 9 .
Figure 9. AG regulates the IIS pathway and AMPK-associated mRNA expression in C. elegans: (a) effect of AG on skn-1 expressions in C. elegans; (b) effect of AG on daf-2 expressions in C. elegans; (c) effect of AG on daf-16 expressions in C. elegans; (d) effect of AG on sek-1 expressions in C. elegans; (e) effect of AG on sod-3 expressions in C. elegans; (f) effect of AG on sod-5 expressions in C. elegans; and (g) effect of AG on tir-1 expressions in C. elegans.The experiment was repeated at least three times.Note: No common letter in the same treatment group indicates a significant difference (p < 0.05).

Figure 9 .
Figure 9. AG regulates the IIS pathway and AMPK-associated mRNA expression in C. elegans: (a) effect of AG on skn-1 expressions in C. elegans; (b) effect of AG on daf-2 expressions in C. elegans; (c) effect of AG on daf-16 expressions in C. elegans; (d) effect of AG on sek-1 expressions in C. elegans; (e) effect of AG on sod-3 expressions in C. elegans; (f) effect of AG on sod-5 expressions in C. elegans; and (g) effect of AG on tir-1 expressions in C. elegans.The experiment was repeated at least three times.Note: No common letter in the same treatment group indicates a significant difference (p < 0.05).

Figure 10 .
Figure 10.AG inhibits H 2 O 2 -induced malformation rate and cell death in zebrafish embryos: (a) H 2 O 2 -induced damage in zebrafish embryos; (b) effect of AG on the malformation rate of zebrafish embryos; (c) effect of AG on the rate of cellular mortality in zebrafish embryos; (d) cell death of zebrafish embryos without AG and H 2 O 2 ; (e) cell death of zebrafish embryos with H 2 O 2 ; (f) cell death of zebrafish embryos with 50 µg/mL AG; (g) cell death of zebrafish embryos with 250 µg/mL AG; and (h) cell death of zebrafish embryos with 500 µg/mL AG.The experiment was repeated at least three times.Note: No common letter in the same treatment group indicates a significant difference (p < 0.05).

Figure 11 .
Figure 11.AG reduces ROS levels of zebrafish embryos: (a) ROS of zebrafish embryos without AG and H2O2; (b) ROS of zebrafish embryos with H2O2; (c) ROS of zebrafish embryos with 50 µg/mL AG; (d) ROS of zebrafish embryos with 250 µg/mL AG; (e) ROS of zebrafish embryos with 500 µg/mL AG; and (f) effect of AG on the ROS of zebrafish embryos.The experiment was repeated at least three times.Note: No common letter in the same treatment group indicates a significant difference (p < 0.05).

Figure 11 .
Figure 11.AG reduces ROS levels of zebrafish embryos: (a) ROS of zebrafish embryos without AG and H 2 O 2 ; (b) ROS of zebrafish embryos with H 2 O 2 ; (c) ROS of zebrafish embryos with 50 µg/mL AG; (d) ROS of zebrafish embryos with 250 µg/mL AG; (e) ROS of zebrafish embryos with 500 µg/mL AG; and (f) effect of AG on the ROS of zebrafish embryos.The experiment was repeated at least three times.Note: No common letter in the same treatment group indicates a significant difference (p < 0.05).

Figure 12 .
Figure 12.AG regulates Sirtuins-associated mRNA expression in zebrafish embryos: (a) effect of AG on SIRT1 expressions in zebrafish embryos; (b) effect of AG on SIRT6 expressions in zebrafish embryos; (c) effect of AG on IGFR expressions in zebrafish embryos; and (d) effect of AG on CD3 expressions in zebrafish embryos.The experiment was repeated at least three times.Note: N common letter in the same treatment group indicates a significant difference (p < 0.05).

Figure 12 .
Figure 12.AG regulates Sirtuins-associated mRNA expression in zebrafish embryos: (a) effect of AG on SIRT1 expressions in zebrafish embryos; (b) effect of AG on SIRT6 expressions in zebrafish embryos; (c) effect of AG on IGFR expressions in zebrafish embryos; and (d) effect of AG on CD38 expressions in zebrafish embryos.The experiment was repeated at least three times.Note: No common letter in the same treatment group indicates a significant difference (p < 0.05).

Figure 13 .
Figure 13.The schematic mechanism of action of AG as hormesis nutrition in anti-aging and antioxidant process: The figure represents hormesis and nutrients (AG) and the involvement of skn-1/Nrf2, daf-16, sod-3, SIRT1, and SIRT6 genes to enhance antioxidant activity and stresses resistance and the inhibition of IGFR/daf-2 and CD38 genes via the activation of AMPK/ MAPK/IIS pathway in order to regulate the lifespan of C. elegans and the antioxidant capacity of zebrafish embryos by inducing anti-aging and antioxidant effects dose dependently [70].

Figure 13 .
Figure 13.The schematic mechanism of action of AG as hormesis nutrition in anti-aging and antioxidant process: The figure represents hormesis and nutrients (AG) and the involvement of skn-1/Nrf2, daf-16, sod-3, SIRT1, and SIRT6 genes to enhance antioxidant activity and stresses resistance and the inhibition of IGFR/daf-2 and CD38 genes via the activation of AMPK/ MAPK/IIS pathway in order to regulate the lifespan of C. elegans and the antioxidant capacity of zebrafish embryos by inducing anti-aging and antioxidant effects dose dependently [70].