Pingwei San Ameliorates Spleen Deficiency-Induced Diarrhea through Intestinal Barrier Protection and Gut Microbiota Modulation

Pingwei San (PWS) has been used for more than a thousand years as a traditional Chinese medicine prescription for treating spleen-deficiency diarrhea (SDD). Nevertheless, the exact mechanism by which it exerts its antidiarrheal effects remains unclear. The objective of this investigation was to explore the antidiarrheal efficacy of PWS and its mechanism of action in SDD induced by Rhubarb. To this end, UHPLC-MS/MS was used to identify the chemical composition of PWS, while the body weight, fecal moisture content, and colon pathological alterations were used to evaluate the effects of PWS on the Rhubarb-induced rat model of SDD. Additionally, quantitative polymerase chain reaction (qPCR) and immunohistochemistry were employed to assess the expression of inflammatory factors, aquaporins (AQPs), and tight junction markers in the colon tissues. Furthermore, 16S rRNA was utilized to determine the impact of PWS on the intestinal flora of SDD rats. The findings revealed that PWS increased body weight, reduced fecal water content, and decreased inflammatory cell infiltration in the colon. It also promoted the expression of AQPs and tight junction markers and prevented the loss of colonic cup cells in SDD rats. In addition, PWS significantly increased the abundance of Prevotellaceae, Eubacterium_ruminantium_group, and Tuzzerella, while decreasing the abundance of Ruminococcus and Frisingicoccus in the feces of SDD rats. The LEfSe analysis revealed that Prevotella, Eubacterium_ruminantium_group, and Pantoea were relatively enriched in the PWS group. Overall, the findings of this study indicate that PWS exerted a therapeutic effect on Rhubarb-induced SDD in rats by both protecting the intestinal barrier and modulating the imbalanced intestinal microbiota.


Introduction
Spleen-deficiency diarrhea (SDD) is a prevalent gastrointestinal condition in traditional Chinese medicine (TCM) characterized by diarrhea as the primary symptom. In TCM, Qi is believed to be a vital force that drives various biological activities in the human body, including the functioning of organs and the transport of nutrients. For many gastrointestinal disorders, it is believed that Qi may become diminished or depleted [1]. The primary causes of SDD are believed to be the consumption of Qi, spleen insufficiency, stomach disharmony, and endophytic dampness, which cause intestinal conductive dysfunction [2]. Due to the weakened spleen's inability to transport and the weakened stomach's failure to digest and process food, the water and food are retained, affecting the ascent of the spleen yang, which eventually collapses, resulting in diarrhea. SDD is characterized by diarrhea, which is the

Animal Models and Treatment
Male Sprague Dawley (SD) rats that were 8 weeks old and of SPF grade were procured from SPF Biotechnology Co., Ltd (Beijing, China). (SYXK 2019-0010). The rats were acclimated for one week at 25 • C, 45-50% humidity, and a 12 h light/dark cycle. The animal trials were executed in accordance with the regulations specified by the Institutional Animal Care and Use Committee of China Agricultural University (AW010203202-2-3).
A total of 35 rats were randomly divided into five groups: control, SDD, PWS-L (1.5 g/kg), PWS-H (3 g/kg) [22], and SLG (dose settings refer to previous studies) [3]. All groups except the control group were administered rhubarb-derived liquid at a dose of 20 mL/kg twice per day (morning and evening) at an 8 h interval for a total of 7 days. Then, the corresponding drugs were administered to each group for 5 days. The control group was administered an equivalent amount of physiological saline. At the end of the trial, the colons were extracted, washed with phosphate-buffered saline (PBS), and assessed. Blood samples were collected from the rats and preserved at −80 • C. The colons and feces were cryopreserved at −80 • C until further analysis.

Record of Body Weights and Fecal Moisture Content
The weight of the rats and the moisture content of their feces were recorded. On Day 3 and Day 8, fecal samples were collected and weighed. Afterward, the feces were dried for 24 h at 60 • C and weighed again.
Fecal moisture content (%) = weight be f ore drying − weight a f ter drying weight be f ore drying × 100%

D-Xylose Absorption and Excretion Assay
The method used for the assay was adapted from previous studies [23,24]. The rats were fasted for 4 h and given 2 mL of 3% d-xylose (Macklin, Shanghai, China) orally. One hour after the intervention, blood samples were collected from the post-ocular vein, and the serum was isolated through centrifugation. The concentration of d-xylose in the serum was determined using the d-xylose Assay Kit (Jiancheng, Nanjing, China) following the manufacturer's instructions.

Histopathological Observation and Alcian Blue-Periodic Acid Schiff (AB-PAS) Staining
The colon tissue was fixed in a solution of 4% paraformaldehyde, embedded in paraffin, sliced into 4-µm sections, and stained with hematoxylin and eosin (H & E) stain (Solarbio, Beijing, China). The stained tissues were examined using a microscope (Olympus, Tokyo, Japan) and the pathological changes were observed through a light microscope (Nikon Eclipse CI, Tokyo, Japan).
Samples were stained with Alcian Blue solution (Solarbio, Beijing, China) and treated with periodic acid and Schiff's reagent for AB-PAS staining. Hematoxylin was used to stain the nuclei lightly. Acid alcohol was used to differentiate the samples, which were then washed with Scott's tap water. Finally, the samples were dehydrated, cleared, and mounted on glass slides with coverslips. The number of goblet cells was counted under a light microscope.

Immunohistochemistry (IHC)
The endogenous peroxidase activity was removed from the paraffin sections by treating them with 3% hydrogen peroxide. Afterward, the sections were blocked with 5% BSA and incubated with primary antibodies, including AQP3 (1:100, Bioss, Beijing, China), AQP4 (1:100, Bioss, Beijing, China), and AQP8 (1:100, Bioss, Beijing, China), at 4 • C for 10 h. After that, the sections were rinsed thrice with TBS and treated with the secondary antibody at 37 • C. The results were captured using a light microscope (DS-Ri2, Nikon, Japan), and the findings were quantified by utilizing Image-Pro Plus 6.0 software.

Enzyme-Linked Immunosorbent Assay (ELISA)
In order to obtain the 10% colon homogenate, 100 milligrams of colon tissue were combined with 900 microliters of normal saline, and subsequently subjected to centrifugation at 3500× g for a duration of 15 min. Inflammatory markers such as TNF-α, IL-1β, and IL-6 proteins were measured using ELISA kits for each corresponding protein.
2.9. Measurement for T-AOC, GSH-Px, and Superoxide Dismutase (SOD) Activities in the Colon, Serum of Rats About 50 mg of colon tissue samples were taken and mixed with 0.5 mL of chilled Tris buffer. The mixture was then homogenized and centrifuged at 3000× g for 15 min at 4 • C, and the resulting supernatant was collected. To determine the levels of T-AOC (A015-2-1), GSH-Px (A005-1-2), and SOD (A001-3) activities in both the colon and serum, commercially available kits (Jiancheng, Nanjing, China) were used as per the manufacturer's instructions.

The Permeability Test of Intestinal Mucosal Barrier
To evaluate the permeability of the intestinal epithelial barrier, rats were subjected to a fluorescein isothiocyanate-labeled dextran permeability test after being anesthetized for 30 min. A section of the ileum, measuring 10 cm in length, was tied off at both ends of the abdominal wall's midline. Subsequently, a solution of 1 mL 4 KD dextran (10 mg/mL, Sigma-Aldrich, St. Louis, MO, USA) labeled with fluorescein isothiocyanate was injected into the intestine. After 30 min, the arterial blood was collected. The upper plasma was analyzed using a fluorescence enzyme labeling instrument (PerkinElmer, Waltham, MA, USA) at 480 nm emission light to assess changes in the intestinal mucosal barrier's permeability.
Concurrently, colon tissue was isolated and quickly frozen on dry ice after embedding in Tissue-Tek O.C.T compound (Sakura, Tokyo, Japan). A frozen section with a thickness of 10 µm was prepared using the CM1950 cryostat (Leica Biosystem, Wetzlar, Germany) and then observed under a fluorescence microscope (Olympus CKX53, Tokyo, Japan) for image acquisition.

Quantitative Reverse-Transcription (qRT)-PCR for the Gene Expression
RNA was isolated from colon tissues using RNA extraction (Promega, Shanghai, China) and subjected to reverse transcription using the GoScript™ Reverse Transcription System (Promega, Shanghai, China). To quantitatively analyze the genes, (qRT)-PCR was performed with the primer sequences for the related genes specified in Table 2. The 2 −∆∆CT method was used to determine the relative expression of the target genes.

Determination of Fecal Flora Diversity
The methodology utilized in this study was based on our previous work [25]. In brief, fecal samples were subjected to DNA extraction and assessed for quality using spectrophotometry. PCR was then conducted (KAPA HiFi HotStart ReadyMix PCR Kit) with DNA polymerase and a diluted genomic DNA template. Specific primers (341F: 5 -CCTAYGGGRBGCASCAG-3 and 806R: 5 -GGACTACNNGGGTATCTAAT-3 ) were used to amplify the V3-V4 region. The Sibiocore platform was utilized to evaluate operational taxonomic unit clusters, and species classification analyses, as well as αand β-diversity. The sequencing data was analyzed using QIIME 1.80, and the VSEARCH (v2.3.4) software was used to cluster sequences with 97% similarity into operational taxonomic units (OTUs). The GreenGene database was used for annotation purposes. For alpha diversity analysis, the mothur (v1.31.2) software was used. To assess the similarity of microbial relative abundance, PCA and PCOA analyses were conducted.

Statistical Analysis
The data was expressed using the mean and standard deviation (SD) and analyzed with the SPSS (Version 26; IBM, Armonk, NY, USA). Statistical significance was determined by conducting one-way analysis of variance (ANOVA), followed by SNK post-test where p < 0.05 was considered statistically significant. The graphics were created using GraphPad Prism (Version 9; La Jolla, CA, USA).

Chemical Profile of PWS
To determine the primary chemical constituents of PWS, UHPLC-MS/MS was utilized to analyze the relevant signals. A total of 287 chemical compounds were detected in PWS and were categorized into five major groups: flavonoids, terpenoids, coumarins, alkaloids, and glycosides (Table S1). The total ion chromatogram of PWS was displayed in Figure 1A,B. Through comparison with the reference sample's retention time, as well as ultraviolet and mass spectrometry, Atractylenolide III, Atractylenolide II, Atractylenolide I, Magnolol, Honokiol, Hesperidin, Liquiritin, and Glycyrrhizic acid were identified (Table 3).
PWS and were categorized into five major groups: flavonoids, terpenoids, coumarins, alkaloids, and glycosides (Table S1). The total ion chromatogram of PWS was displayed in Figure 1A,B. Through comparison with the reference sample's retention time, as well as ultraviolet and mass spectrometry, Atractylenolide III, Atractylenolide II, Atractylenolide I, Magnolol, Honokiol, Hesperidin, Liquiritin, and Glycyrrhizic acid were identified (Table 3).

PWS Ameliorates SDD Symptoms in Rats
The animal experiment scheme and design are illustrated in Figure 2A. Compared to the control group, the SDD group showed a slower rate of weight gain; however, all rats gained weight faster after PWS administration ( Figure 2B). The administration of rhubarb caused diarrhea in rats and increased their fecal water content. In contrast, rats treated with PWS had significantly lower fecal water content than those in the SDD group ( Figure 2C,D). Rhubarb treatment resulted in reduced colon length, which was markedly improved by different doses of PWS ( Figure 2E).
The animal experiment scheme and design are illustrated in Figure 2A. Compar the control group, the SDD group showed a slower rate of weight gain; however, a gained weight faster after PWS administration ( Figure 2B). The administration of rhu caused diarrhea in rats and increased their fecal water content. In contrast, rats tr with PWS had significantly lower fecal water content than those in the SDD group (F 2C,D). Rhubarb treatment resulted in reduced colon length, which was markedl proved by different doses of PWS ( Figure 2E).

PWS Suppresses Inflammation and Inflammation-Induced Oxidation in SDD Rats
The SDD group exhibited inflammation infiltration (black arrows) and disordered cell arrangement (red arrows) in colon tissues compared to the control group. However, treatment with PWS had a protective effect on SDD-induced diarrhea in rats, resulting in normal mucosal shape and intact epithelium in the intestine and colon tissues ( Figure 3A). The impact of PWS on the expression of colonic cytokines (TNF-α, IL-6, and IL-1β) in SDD rats was analyzed to examine its effects on pathological inflammation. As indicated in Figure 3B, the expression levels of TNF-α, IL-6, and IL-1β were significantly increased in the model group, while PWS administration significantly decreased the expression levels (p < 0.01). To determine the oxidative stress biomarkers, TAOC levels and the activities of GSH-Px and SOD in the colon and serum were assessed. The results showed that SDD diarrhea significantly reduced the levels of TAOC and the activities of GSH-Px and SOD. Nevertheless, PWS notably alleviated the oxidative stress damage caused by SDD in the colon tissues (p < 0.01) ( Figure 3C,D). The levels of TAOC, GSH-Px, and SOD activities were significantly increased in the PWS-treated group compared to the control group, indicating that PWS inhibited inflammation and inflammation-induced oxidation.
(p < 0.01). To determine the oxidative stress biomarkers, TAOC levels and the activities of GSH-Px and SOD in the colon and serum were assessed. The results showed that SDD diarrhea significantly reduced the levels of TAOC and the activities of GSH-Px and SOD. Nevertheless, PWS notably alleviated the oxidative stress damage caused by SDD in the colon tissues (p < 0.01) ( Figure 3C,D). The levels of TAOC, GSH-Px, and SOD activities were significantly increased in the PWS-treated group compared to the control group, indicating that PWS inhibited inflammation and inflammation-induced oxidation.

PWS Restored Flora Diversity to Curb SDD
The use of natural active products can influence changes in the intestinal flora, which may contribute to the development of SDD. SDD and PWS were found to alter the alpha diversity (Shannon, Simpson, Chao, and ace) of the fecal flora ( Figure 4A-D). The Venn diagram was used to identify common or unique taxa among the different groups, and a total of 379 OTUs in the control group, 287 ZOTUs in the SDD group, 397 OTUs in the PWS-H group, 252 OTUs in the PWS-L group, and 289 OTUs in the SLG group were detected ( Figure 4E). The species bar chart was used to show the changes in species composition and proportion among groups. SDD reduced the relative abundance of Firmicutes and increased that of Bacteroidetes, while PWS reversed the changes ( Figure 4F). The NMDS analysis and plots of PCA indicated that the control, SDD, and PWS groups had distinct community compositions of fecal flora, and PWS was able to normalize the bacterial community after SDD ( Figure 4G). Figure 4H displays the genus-level relative abundance of intestinal flora in rats. In comparison to the control group, the SDD group exhibited an increase in the relative abundance of Ruminococcus and Frisingicoccus and a decrease in the relative abundance of Prevotellaceae, Eubacterium_ruminantium_group, and Tuzzerella (p < 0.01). Treatment with PWS restored the relative abundance of related bacterial genera in SDD rats to normal levels. We identified a significant difference in the dominance of bacterial communities among the five groups by using lLEfSe. According to the analysis results, Facklamia and Oscillospira were the key bacterial types that caused an imbalance in intestinal microflora in the SDD group. However, Prevotella, Eubacterium_ruminantium_group, and Pantoea were relatively enriched in the PWS group ( Figure 4I).

PWS Increases the Expression of Aquaporin3 (AQP3), Aquaporin4 (AQP4), and Aquaporin8 (AQP8)
Diarrhea is commonly associated with abnormal water metabolism in the intestine, which is regulated by AQP3, AQP4, and AQP8 proteins. In this study, we analyzed the expression of these proteins in colon tissue and found that their expression was significantly reduced in the SDD group (p < 0.05) but significantly increased in the PWS and SLG groups (p < 0.01) ( Figure 5A-C). These findings were confirmed by qPCR results (Figure

PWS Increases the Expression of Aquaporin3 (AQP3), Aquaporin4 (AQP4), and Aquaporin8 (AQP8)
Diarrhea is commonly associated with abnormal water metabolism in the intestine, which is regulated by AQP3, AQP4, and AQP8 proteins. In this study, we analyzed the expression of these proteins in colon tissue and found that their expression was significantly reduced in the SDD group (p < 0.05) but significantly increased in the PWS and SLG groups (p < 0.01) ( Figure 5A-C). These findings were confirmed by qPCR results (Figure 5D-F).

PWS Relieved SDD by Maintaining Goblet Cell Function and Restoring the Intestinal Mucus Barrier
Regarding mucosal permeability, we evaluated the FD4 index, which reflects gut barrier function. Results showed that PWS reduced mucosal permeability, as evidenced by decreased fluorescence permeability of FITC-dextran and lower FD4 levels in serum (p < 0.01) ( Figure 6A). The mucosal barrier of the intestine serves as the primary defense mechanism for safeguarding the intestinal epithelium. SDD reduced the number of goblet cells, but PWS and SLG a enuated this depletion ( Figure 6B). Additionally, SDD rats had lower levels of D-xylose in their plasma, while rats given PWS had higher levels (p < 0.01) ( Figure  6C). Furthermore, PWS upregulated the expression of Ocln and Zo-1, which were downregulated in the SDD group ( Figure 6D,E). These results indicate that PWS protects goblet

PWS Relieved SDD by Maintaining Goblet Cell Function and Restoring the Intestinal Mucus Barrier
Regarding mucosal permeability, we evaluated the FD4 index, which reflects gut barrier function. Results showed that PWS reduced mucosal permeability, as evidenced by decreased fluorescence permeability of FITC-dextran and lower FD4 levels in serum (p < 0.01) ( Figure 6A). The mucosal barrier of the intestine serves as the primary defense mechanism for safeguarding the intestinal epithelium. SDD reduced the number of goblet cells, but PWS and SLG attenuated this depletion ( Figure 6B). Additionally, SDD rats had lower levels of D-xylose in their plasma, while rats given PWS had higher levels (p < 0.01) ( Figure 6C). Furthermore, PWS upregulated the expression of Ocln and Zo-1, which were downregulated in the SDD group ( Figure 6D,E). These results indicate that PWS protects goblet cell function and restores the mucus barrier in chronic colitis.

Discussion
PWS is a traditional Chinese medicine formula that has been used for gastrointe ailments for many years. Our results demonstrate that PWS mostly improved SD inhibiting the generation of inflammatory substances (TNF-α, IL-1β, and IL-6), enha the body's antioxidant potential (TAOC, GSH-Px, and SOD), increasing the express aquaporins (AQP3, AQP4, and AQP8) and tight junction proteins (ZO-1 and Occl and stimulating mucin secretion from goblet cells in the colon. In addition, PWS s cantly increased the abundance of Prevotellaceae, Eubacterium_ruminantium_group Tuzzerella, while reducing the abundance of Ruminococcus and Frisingicoccus in the fe SDD rats. Through the LEfSe analysis, Prevotella, Eubacterium_ruminantium_group Pantoea were relatively enriched in the PWS group. Overall, the findings of this stu dicate that PWS exerted a therapeutic effect on Rhubarb-induced SDD in rats by bot tecting the intestinal barrier and modulating the imbalanced intestinal microbiota. It is common to use rhubarb to induce a model of SDD [3,26]. Rhubarb anth nones (RA) are considered primary laxative elements, which fall under the categ anthranoid glycosides and encompass different types of anthraquinone derivatives as sennoside A, B, C, and D [27]. The primary laxative component is believed to be s side A (SA) [28]. In this study, the symptoms observed in the experimental group consistent with those of SDD, indicating the successful establishment of the model research has demonstrated that diarrhea is typically accompanied by intestinal infla tion, severe dehydration, and intestinal barrier damage [29,30]. Decreased express

Discussion
PWS is a traditional Chinese medicine formula that has been used for gastrointestinal ailments for many years. Our results demonstrate that PWS mostly improved SDD by inhibiting the generation of inflammatory substances (TNF-α, IL-1β, and IL-6), enhancing the body's antioxidant potential (TAOC, GSH-Px, and SOD), increasing the expression of aquaporins (AQP3, AQP4, and AQP8) and tight junction proteins (ZO-1 and Occludin), and stimulating mucin secretion from goblet cells in the colon. In addition, PWS significantly increased the abundance of Prevotellaceae, Eubacterium_ruminantium_group, and Tuzzerella, while reducing the abundance of Ruminococcus and Frisingicoccus in the feces of SDD rats. Through the LEfSe analysis, Prevotella, Eubacterium_ruminantium_group, and Pantoea were relatively enriched in the PWS group. Overall, the findings of this study indicate that PWS exerted a therapeutic effect on Rhubarb-induced SDD in rats by both protecting the intestinal barrier and modulating the imbalanced intestinal microbiota.
It is common to use rhubarb to induce a model of SDD [3,26]. Rhubarb anthraquinones (RA) are considered primary laxative elements, which fall under the category of anthranoid glycosides and encompass different types of anthraquinone derivatives, such as sennoside A, B, C, and D [27]. The primary laxative component is believed to be sennoside A (SA) [28].
In this study, the symptoms observed in the experimental group were consistent with those of SDD, indicating the successful establishment of the model. Prior research has demonstrated that diarrhea is typically accompanied by intestinal inflammation, severe dehydration, and intestinal barrier damage [29,30]. Decreased expression of tight junction (TJ) proteins such as ZO-1 and Occludin can weaken the intestinal epithelium integrity and cause colitis [31][32][33]. Our investigation demonstrated that the expression levels of ZO-1 and Occludin were diminished in the colonic tissue of rats with SDD. However, the administration of PWS notably ameliorated the expression of these proteins, thus enhancing the integrity of the intestinal epithelium and improving intestinal permeability. AQPs are transporters present in membranes that facilitate water absorption, secretion, and metabolism in the intestine. They may also regulate the tight junctions of the intestinal mucosa [34].
Our results indicate that rats with SDD exhibited a diminished mRNA expression of AQP3, AQP4, and AQP8 in the colon compared to the control group. Nevertheless, the use of PWS treatment heightened the expression of AQPs, resulting in water reabsorption within the colon and a decrease in fecal moisture.
Intestinal inflammation and oxidative stress are widely recognized as key factors contributing to the development of mucosal barrier dysfunction, which can result in a range of gastrointestinal disorders [35,36]. Inflammation triggers the release of proinflammatory cytokines such as TNF-α, IL-1β, and IL-6 by immune cells, which can harm the intestinal epithelium and compromise the mucosal barrier [37]. Our investigation demonstrated that rats with SDD exhibited extensive inflammation infiltration in their colon tissue, coupled with tissue structure loss and reduced goblet cell count. However, PWS intervention mitigated the damage. Additionally, the results revealed a noteworthy elevation in the expression of proinflammatory cytokines (TNF-α, IL-1β, and IL-6) in rats with SDD. Nonetheless, the administration of PWS reduced the expression of these inflammatory cytokines. In addition to inflammation, oxidative stress is another important contributor to mucosal barrier dysfunction. In the gut, oxidative stress can lead to increased intestinal permeability, inflammation, and damage to the mucosal barrier [38]. GSH-Px, SOD, and TAOC are antioxidant enzymes that play important roles in protecting the body from oxidative stress. Previous studies have shown that animals with diarrhea often experience increased oxidative stress and decreased levels of GSH-Px, SOD, and TAOC in their bodies [39,40]. This suggests that oxidative stress may contribute to the development of diarrhea and that these antioxidant enzymes may be potential targets for the treatment of diarrhea. Our current data presented that PWS could significantly upregulate the levels of GSH-Px and TAOC and the activities of SOD in the colons of rats. Atractylenolide III, Atractylenolide II, Atractylenolide I, Magnolol, Honokiol, Hesperidin, Liquiritin, and Glycyrrhizic acid were identified as the key components responsible for the observed benefits of PWS through the UHPLC-MS/MS analysis. Several previous studies have highlighted the anti-inflammatory and antioxidant properties of these components [41][42][43][44]. A study has shown that Atractylenolide-1 was able to improve mucoprotein MUC2 and enhance the expression of tight junction proteins ZO-1 and Occludin in mice with colitis. This treatment also resulted in a significant decrease in pro-inflammatory cytokines TNF-α, IL-6, and IL-1β [45]. Magnolol and honokiol are bioactive compounds found in Magnolia officinalis Rehd. et Wils. that have been demonstrated to alleviate diarrhea and intestinal damage by regulating the calcium-activated potassium channels signaling pathway [46]. Taken together, PWS exerted a therapeutic effect on Rhubarb-induced SDD by repairing the intestinal barrier through anti-inflammation and anti-oxidation.
The gut's microbial community can positively impact host health by regulating nutrient metabolism, immune system modulation, and prevention of pathogenic colonization [47]. This investigation examined fecal bacterial changes in rats with diarrhea and their relationship. In rats with diarrhea, richness, evenness, and diversity were lower than in healthy rats, and the structure and membership of the bacterial community differed. Treatment with PWS restored the proportion of related bacterial genera in SDD rats to normal levels. Moreover, PWS significantly increased the abundance of Prevotellaceae, Eubac-terium_ruminantium_group, and Tuzzerella, while reducing the abundance of Ruminococcus and Frisingicoccus in the feces of SDD rats. Through the LEfSe analysis, Prevotella, Eu-bacterium_ruminantium_group, and Pantoea were relatively enriched in the PWS group. Prevotellaceae is a crucial intestinal cornerstone bacterial genus that is closely associated with intestinal health [48]. The presence of Prevotellaceae in the gut can prevent the M1like polarization of colonic macrophages and the production of proinflammatory factors, such as IL-1β and TNF-α [49]. Eubacterium can increase the bioavailability of butyrate to colonic epithelial cells [50]. Additionally, some studies have suggested that short-chain fatty acids (SCFAs) can exhibit anti-inflammatory effects by inhibiting pro-inflammatory cytokines, such as IFN-γ, IL-1β, IL-6, IL-8, and TNF-α, while increasing the expression of anti-inflammatory cytokines, such as IL-10 and TGF-β [51]. The bioactive compounds Liquiritin and Glycyrrhizic acid found in Shaoyao-Gancao-Tang has been demonstrated to decrease the abundance of bacteria of the genera Ruminococcus [52]. This finding is consistent with our study. Taken together, PWS exerted a therapeutic effect on Rhubarb-induced SDD by balancing the intestinal flora.

Conclusions
PWS significantly increased body weight, decreased fecal water content, and reduced inflammatory cell infiltration in the colon. It also alleviated colitis and promoted the expression of AQPs and tight junction markers, inhibiting the loss of colonic cup cells in rats with SDD induced by Rhubarb. In addition, PWS significantly increased the abundance of Prevotellaceae, Eubacterium_ruminantium_group, and Tuzzerella, while reducing the abundance of Ruminococcus and Frisingicoccus in the feces of SDD rats. Through the LEfSe analysis, Prevotella, Eubacterium_ruminantium_group, and Pantoea were relatively enriched in the PWS group. Overall, the findings of this study indicate that PWS exerted a therapeutic effect on Rhubarb-induced SDD in rats by both protecting the intestinal barrier and modulating the imbalanced intestinal microbiota. The exact mechanisms underlying the beneficial effects of PWS on SDD and its influence on the composition of gut microbiota remain unclear. Further research is needed to gain a better understanding of the underlying mechanisms that account for the therapeutic benefits of PWS treatment.

Author Contributions:
The study design was developed by Z.H. and Y.F., and the initial draft of the manuscript was written by Y.F., Q.Z. and H.W. The manuscript was later revised by Z.H. and Y.W. Y.F. conducted most of the experiments and analyzed the data, with support from Y.Z. and Y.G. in data interpretation and manuscript editing. All authors have read and agreed to the published version of the manuscript. Informed Consent Statement: Not applicable.

Data Availability Statement:
The data presented in this study are available upon request from the corresponding author.