Elevation of Fatty Acid Biosynthesis Metabolism Contributes to Zhongshengmycin Resistance in Xanthomonas oryzae
Abstract
:1. Introduction
2. Results
2.1. Phenotypes of Xoo-Rzs
2.2. Difference in Metabolic Spectrum between Xoo-Rzs and Xoo-S
2.3. Enrichment of Metabolic Pathways in Xanthomonas Oryzae
2.4. Biomarkers of ZS Resistance
2.5. Increased Fatty Acid Biosynthesis in Xoo-Rzs
2.6. Exogenous Palmitic Acid Promotes the Resistance of Xanthomonas Oryzae to ZS
3. Discussion
4. Materials and Methods
4.1. Strain Characteristics and MIC Measurement
4.2. Sample Preparation and Parameter Setting for the GC–MS Analysis
4.3. Technical Tools and Software Used for Data Management
4.4. Determination of the Activity of ACC
4.5. Assay of the Gene Expression Levels In Vivo
4.6. Antibiotic Bactericidal Assay
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Wang, Q.; Lin, M.; Shen, P.; Guan, Y. Elevation of Fatty Acid Biosynthesis Metabolism Contributes to Zhongshengmycin Resistance in Xanthomonas oryzae. Antibiotics 2021, 10, 1166. https://doi.org/10.3390/antibiotics10101166
Wang Q, Lin M, Shen P, Guan Y. Elevation of Fatty Acid Biosynthesis Metabolism Contributes to Zhongshengmycin Resistance in Xanthomonas oryzae. Antibiotics. 2021; 10(10):1166. https://doi.org/10.3390/antibiotics10101166
Chicago/Turabian StyleWang, Qiaoxing, Meiyun Lin, Peihua Shen, and Yi Guan. 2021. "Elevation of Fatty Acid Biosynthesis Metabolism Contributes to Zhongshengmycin Resistance in Xanthomonas oryzae" Antibiotics 10, no. 10: 1166. https://doi.org/10.3390/antibiotics10101166