Maternal Folic Acid Supplementation Differently Affects the Small Intestinal Phenotype and Gene Expression of Newborn Lambs from Differing Litter Sizes

Simple Summary Maternal folic acid (FA) level during gestation is a factor affecting fetal development due to the vital role of FA in nucleotide synthesis. As a key organ of lamb, the development of the small intestine during gestation in response to maternal FA is not clear. Therefore, the current study evaluated the intestinal phenotype and development-, apoptosis- and digestion-related genes expression in newborn lambs whose mothers supplemented FA during gestation. FA supplementation improved the ratio of small intestinal weight to live body weight, intestinal muscle layer thickness and the expression of IGF-I, BCL-2 and SGLT1. Meanwhile, triplets indicated poorer morphological development compared to twins, but the expression of several genes was higher in twins compared to triplets. Overall, maternal FA supplementation during gestation increased the development of offspring’s small intestinal. Abstract The purpose of this study was to investigate the effect of maternal dietary folic acid (FA) supplementation during gestation on small intestinal development of newborn lambs of different litter sizes, focusing on the intestinal morphology and development-, apoptosis- and digestion-related genes expression. One hundred and twenty Hu ewes (Ovis aries) were inseminated and randomly allotted to three groups. One group received a control diet [without FA supplementation, control (CON)] and the other two groups received control diets supplemented with different amount of FA [16 or 32 mg FA per kg dry matter (DM), i.e., F16 and F32] during pregnancy. After lambing, according to the dietary FA levels and litter size (twins, TW; triplets, TR), the neonatal lambs were divided into 6 (TW-CON, TW-F16, TW-F32, TR-CON, TR-F16, TR-F32) treatment groups. The results showed that the ratio of small intestinal weight to live body weight and the thickness of the intestinal muscle layer in the offspring was enhanced significantly with increasing maternal FA supplementation (p < 0.05). Meanwhile, the expression levels of insulin-like growth factor I (IGF-I), B-cell lymphoma-2 (BCL-2) and sodium/glucose co-transporter-1 (SGLT1) in the small intestines of the newborn lambs were increased, while the opposite was true for Bcl2-associated × (BAX) in response to FA supplementation (p < 0.05). Moreover, the small intestinal weights of twins were significantly higher than those of triplets (p < 0.01), and the expression levels of IGF-I (p < 0.05), sucrase-isomaltase (SI) (p < 0.05) and solute carrier family 2 member 5 (SLC2A5) (p < 0.01) were significantly lower than those in triplets. These findings suggest that maternal FA supplementation could improve the offspring’s small intestinal phenotype and the expression of development-, apoptosis- and digestion-related genes, so it could promote the small intestinal development of newborn lambs. Furthermore, the small intestine phenotypic development of twins was generally better than that of triplets, while the expression levels of the above genes of twins were lower than those of triplets.


Data and Sample Collection
After the birth of lambs, the litter size of all ewes was recorded, and only twins and triplets were selected for this experiment, of which 66 ewes met the requirements for lambing twins and triplets. The number of ewes that gave birth to twins and triplets were 16 and 5, 13 and 13, and 10 and 9 for the CON, F16 and F32 groups, respectively. The number of twins and triplets used in this experiment were 15 and 6, 11 and 11, and 8 and 14 for the CON, F16 and F32 groups, respectively. DMI was similar throughout the entire gestation period (both early and late stages) in all treatment groups (p > 0.05). There was no significant difference between the initial weight of ewes giving birth to twins (44.00 ± 0.53 kg) and triplets (44.20 ± 0.57 kg; p > 0.05). Maternal FA supplementation had no effect on the average postpartum weight of ewes, but the postpartum weight of ewes giving birth to triplets was significantly lower than that of ewes giving birth to twins [15].
After lambing, sixty-five twins and triplets with near-average birth weights were euthanized after birth by carbon dioxide indrawing and then followed by exsanguination. After the opening of abdominal cavity and isolation of the small intestine, it was rinsed with normal saline and weighed. The proximal portion of the duodenum and jejunum (a piece approximately 2 cm long) were then removed, split gently using a scalpel, rinsed clean with precooled physiological saline and fixed in 4% paraformaldehyde solution, replacing the fixative after 24 h. Subsequently, approximately 2 cm of anterior duodenum and jejunum samples from each animal were collected in RNase-free cryopreservation tubes and stored in liquid nitrogen for qRT-PCR test.

Small Intestinal Morphology
The anterior segment specimens stored in 4% paraformaldehyde solution were paraffin-embedded and sectionalized, and intestinal morphological analysis was performed with eosin and hematoxylin staining (n = 6 for each group). Villous heights, thickness of intestinal muscle layer and crypt depths of intestine for each lamb were measured at intact and well-oriented villi using scanned images of six separate areas obtained from the front part of the duodenum and jejunum were examined at 400× magnification. The measurements were done by means of an imaging software (Panoramic Viewer, 3DHISTECH Ltd., Budapest, Hungary).

qRT-PCR
Total RNA was extracted from the duodenal and jejunal section of the newborn lambs (n = 6 for each treatment) using an RNA extraction kit (Tiangen, Biochemical Technology Co., Ltd, Beijing, China).
The extracted RNA was dissolved in RNase-Free ddH 2 O. A Nanodrop spectrophotometer (Implen, Inc., Westlake Village, CA, USA) was used to measure the purity of RNA (OD260/280 ratio). First, strand cDNA was synthesized from the RNA through reverse transcription using FastQuant DNA first-chain synthesis kit (Tiangen, Biochemical Technology Co., Ltd, Beijing, China). The reaction was carried out in a total volume of 20 µL containing 2 µL of 10× Fast RT Buffer, 1µL of RT Enzyme Mix, 2 µL of FQ-RT Primer Mix, 2 µL 5× gDNA Buffer, 8 µL of total RNA and 5 mL of RNase-free ddH 2 O. The qRT-PCR was performed on an iQ5 system (Bio-Rad, Hercules, CA, USA) by using the 2× SYBR Green Qpcr Mix kit (Tiangen, Biochemical Technology Co., Ltd, Beijing, China), which was aimed to analyze the expression of development-(IGF-I, GLP2R and EGF), apoptosis-(BCL-2 and BAX) and digestion-related (MGAM, PEPT1, SLC2A2, LCT, SGLT1, SI and SLC2A5) genes in the front duodenal and jejunal segment of newborn lambs [18][19][20][21][22][23][24]. The 25 µL Real-Time PCR reaction mix contained 12.5 µL 2× SuperReal Premix Plus, 0.5 µL Forward Primer (10 µM), 0.5 µL, Reverse Primer (10 µM), 1 µL cDNA and 10.5 µL RNase-free ddH 2 O. The design and synthesis of primers were made by Shanghai Generay Biotech Co., Ltd (Shanghai, China). The sequences of the forward and reverse primers are shown in Table 2, and the housekeeping gene GAPDH was used to calculate the relative expression levels of each target gene in the two tissues. The qRT-PCR used the value of the threshold cycle (Ct) for the relative quantification of gene amplification. The expression level of the target gene relative to those of GAPDH gene was quantified using the Ct value comparison method of the 2 −∆∆Ct method.

Statistical Analysis
The data of weight and morphology of the small intestine and genes expression were analyzed using the generalized linear model (GLM) of statistical package SPSS version 23.0 (SPSS, IBM, Inc., Chicago, IL, USA) to assess the effects of litter size (TW and TR) and dietary FA supplementation levels (CON, F16 and F32). Treatment means were compared using Duncan's new multiple range test with a significance level of p < 0.05. Polynomial analysis was conducted to test the linear or quadratic response to the levels of FA supplementation in the diet at the 0.05 level. The variables in different litter sizes were compared using the independent samples T-test at the 0.05 level. Results are shown as mean ± SEM. The relationships between genes and phenotypes for the various treatments were determined using correlation analysis.

Weight and Morphology of the Small Intestine
The effect of litter size and FA supplementation on intestinal morphology is shown in Table 3. The small intestinal weight of twin lambs was significantly higher than that of the triplet lambs (p = 0.001). In addition, the ratio of small intestine weight to live body weight increased linearly with the FA supply in maternal diet (p = 0.005). CON, F16 and F32 mean newborn lambs from ewes fed 0, 16 or 32 mg (kg DM) −1 folic acid in the basal diet, respectively. I×, interaction effects of litter size and folic acid addition. (1) The ratio of small intestine weight to live body weight linearly increased response to folic acid supplementation (p = 0.005).
The results and image of the morphology of duodenal segments are presented in Figures 1-3, respectively. There was a significant (p = 0.011) effect of the interaction between litter size and FA supplementation on duodenal muscle layer thickness of the lambs. The thickness of duodenal muscle layer increased linearly with maternal dietary FA supplementation in the twin-born lambs (p < 0.001), while FA supplementation had no influence on the duodenal muscle layer thickness among the triplet-born lambs (p = 0.134). The FA concentration and litter size had significant effects on jejunal muscle layer thickness. The jejunal muscle layer thickness increased linearly with the increase of FA level (p = 0.019). Meanwhile, jejunal muscle layer thickness of triplets was significantly higher than twins (p = 0.019). The villus height and crypt depth of duodenum and jejunum tissue were similar among the treatment groups (p > 0.05).
Animals 2020, 10, x FOR PEER REVIEW 7 of 18 CON, F16 and F32 mean newborn lambs from ewes fed 0, 16 or 32 mg (kg DM) −1 folic acid in the basal diet, respectively. I×, interaction effects of litter size and folic acid addition. 1) The ratio of small intestine weight to live body weight linearly increased response to folic acid supplementation (p = 0.005).

Gene Expression
The effect of litter size and FA supplementation on intestinal development-related genes expression is shown in Table 4. The interaction between FA supplementation and litter size had a significant effect on the expression of IGF-I in the duodenum (p = 0.005). The duodenal expression of IGF-I increased linearly with dietary FA supplementation in triplet-born lambs (p = 0.039), but it was not affected by FA supplementation in twin-born lambs (p = 0.310). Furthermore, the expression of IGF-I in the jejuna of twins was remarkably lower than triplets with no significant interaction effect between litter size and FA supplementation (p = 0.041). However, expression of EGF and GLP2R genes in the small intestine showed no statistical differences among the treatment groups (p > 0.05).
Results of apoptosis-related genes expression are shown in Figure 4. In the duodenum, maternal diet FA supplementation significantly linearly increased the expression of BCL-2 (p = 0.027). For the jejunum, the expression of BAX in newborn lambs was influenced by the interaction effect between litter size and FA supplementation (p = 0.046), which showed a quadratic response to FA supplementation in twin lambs (p = 0.034). 10 5 shows the results of small intestinal digestion-related genes expression of newborn lambs. The interaction between litter siz tation had a significant (p < 0.05) effect on the expression of SGLT1 in the duodenal tissue and on the expression of LCT and SLC2A5 in expression level of SGLT1 increased linearly with the supply of FA in triplet lambs (p = 0.005), while the expression level of SLC2 lly with the FA supplementation in triplets (p = 0.021). Meanwhile, the addition of FA had no effect on the expression of SGLT1 in the SLC2A5 in the jejunal tissue in twin-born lambs (p ＞ 0.05). The expression level of LCT was not affected by FA supplementation in t  Figure 5 shows the results of small intestinal digestion-related genes expression of newborn lambs. The interaction between litter size and FA supplementation had a significant (p < 0.05) effect on the expression of SGLT1 in the duodenal tissue and on the expression of LCT and SLC2A5 in the jejunal tissue. The expression level of SGLT1 increased linearly with the supply of FA in triplet lambs (p = 0.005), while the expression level of SLC2A5 varied quadratically with the FA supplementation in triplets (p = 0.021). Meanwhile, the addition of FA had no effect on the expression of SGLT1 in the duodenal tissue and SLC2A5 in the jejunal tissue in twin-born lambs (p > 0.05). The expression level of LCT was not affected by FA supplementation in triplets (p = 0.511), but it showed a quadratic response to FA supplementation in twins (p = 0.005). Moreover, the effect of litter size on the expression of SI and SLC2A5 was also significant in the duodenal tissue. The expression of SI and SLC2A5 were elevated in the triplets compared to those in the twins (p < 0.05). Results of other genes (MGAM, PEPT1, SLC2A2) with no significant differences in duodenum and jejunum were not shown.  0.511), but it showed a quadratic response to FA supplementation in twins (p = 0.005). Moreover, the effect of litter size on the expression of SI and SLC2A5 was also significant in the duodenal tissue. The expression of SI and SLC2A5 were elevated in the triplets compared to those in the twins (p < 0.05). Results of other genes (MGAM, PEPT1, SLC2A2) with no significant differences in duodenum and jejunum were not shown.

Pearson Correlation Analysis
We also determined the correlations between gene expression and phenotype parameters (villus height, thickness of muscle layer and crypt depth) of the duodenal and jejunal tissues; the results from the correlation analyses are listed in Tables 5 and 6. In the duodena, the expression levels of MGAM, LCT, SGLT1, SI and SLC2A5 were significantly positively correlated with crypt depth (p < 0.05). Similarly, the expression of IGF1 had a positive correlation with jejunal crypt depth (p = 0.027). In addition, positive correlations were found between MGAM and villus height (p = 0.029), as well as between PEPT1 and muscle layer thickness in jejunum (p = 0.008). In general, although there were no significant correlations between thickness of muscle layer and gene expression in the duodenum tissue, there was a general trend of negative correlation. In addition, villus height seems to be a nonsignificant positive correlation with gene expression in the duodenum and jejunum tissue.

Discussion
As a vital methyl donor, FA plays a role in the maintenance and development of the fetus during gestation [25]. Maternal supplementation of methyl donors such as FA has been reported to decrease incidence of pregnancy failure and increase litter size in early parity sows [26]. Unlike the monogastric animals, FA can be synthesized in the rumen of ruminants, so according to the classic ruminant nutrition theory, there is no need for dietary FA given in ruminants [27]. However, the supply of FA at a particular physiological stage is of great importance to the assurance of production performance due to the variation of nutrient requirements at different physiological stages of ruminants. For example, a previous study reported a high demand for FA in the tissues of lactating and gestating cows [28]. The functional maturity of the gastrointestinal system is critical to the survival of the neonate [29]. Insufficient maternal nutrition has a deleterious effect on the small intestine of the offspring. These effects generally include a reduction in the weight and length of the small intestine, resulting in a decrease in the functional area and a perturbed development of the small intestine [30,31]. The weight of small intestine and its proportion of body weight are important indicators of its development. In the present study, it was shown that the increase of the small intestine/live body weight ratio in FA-supplemented groups was the most direct indicator of improved intestinal development because of maternal dietary FA supplementation.
During the morphological assessment of the intestinal tract in livestock, the villus height, crypt depth and muscle layer thickness of the small intestine are important indicators that reflect the ability of gastrointestinal tract to digest and absorb nutrients [32]. The intestinal phenotype of the offspring could be affected by FA concentration in the mother's diet. A previous study has shown that the deficiency of methyl donors at the time of gestation and lactation resulted in increased apoptosis in the crypts and decreased differentiation of villus epithelial cells in 26-day-old rats [10]. Sufficient FA is vital for maintaining the normal structure of the human small intestine [33]. In our experiment, similar results were obtained; supplementation of the ewe's diet with FA during pregnancy markedly improved muscle layer thickness in offspring, suggesting an improvement in intestinal function. Furthermore, it is generally accepted that litter size is also an important factor affecting the development of the offspring [34]. Depending on the number of fetuses present in the uterus, the same maternal nutritional level provides different amount of nutrients for tissue growth, which can lead to obvious discrepancy in fetal development of different litter sizes [31]. Animals with nutritional limitations in the fetus tend to have poorer morphological development of small intestine after birth than the normal-nutrition-supplied animals [35]. In this study, the weight of the small intestine of twins was significantly higher than that of triplets, with the development of the small intestine of twin lambs being superior based on phenotypic evaluation-that is to say, under the same nutrition level of the ewes, the triplets will receive less nutrition from the dam than the twins, so deficiency of nutrition leads to apparent morphological defects in small intestinal development. Finally, since most of the effects of F16 and F32 on small intestine morphological development were not significantly different, the optimal level of FA supplementation on intestine development deserves further study.
The results of this trial showed that not only the morphological aspects of development were affected but also that the supply of FA can influence the genes expression level in the small intestine. Interestingly, the expression of IGF-I in twin lambs was significantly lower than that in triplet lambs, but the result of the small intestinal phenotype was that twins developed better than triplets. Insulin-like growth factors (IGFs) are important growth factors that regulate the development of the small intestine [36]. Due to the role of IGF-I in promoting division and differentiation of cells, it has been found in pigs [37] and sheep [38] that IGF-I supplementation could improve the morphology of small intestine and promote the development of small intestinal epithelium. Comparing with twins, triplets compete more for maternal nutrition during the fetal stage and are more likely to suffer from developmental defects caused by inadequate nutrition. The disturbance of environmental stress caused by undernutrition on ontogenetic development will reduce the adaptability of animals. However, the fetal programming determined by epigenetic modification can be adjusted by influencing the gene expression, so this genetic change caused by gene plasticity that tends to restore the normal phenotype of the body is called "genetic compensation" [39,40]. Therefore, a possible explanation for the higher expression of IGF-I in the jejunal tissue of triplets than twins is that the body compensates for the lower small intestine weight of triplet-born lambs by increasing expression of IGF-I. A similar experiment found that the intestinal morphology of offspring piglets with nutritional restriction during gestation was impaired, while FA supplementation increased the expression of antiapoptotic genes in jejunum tissues [41].
Chronic FA deficiency can lead to an imbalance in the defense system against cell antioxidants and increased apoptosis, indicating that FA is crucial in cell cycle regulation [42]. When we examined the expression of intestinal apoptosis-related genes, we found that in the duodenum, FA addition during gestation could prominently improve the expression of antiapoptotic gene BCL-2 in newborn lambs. There was also a linear decline in the expression of proapoptotic gene BAX with the addition of FA. These results were in consistent with previous reports about the influence of maternal FA supplementation on morphology and apoptosis-related gene expression in the jejuna of newborn intrauterine-growth-retarded piglets [41]. Furthermore, BAX and BCL are important genes that regulate apoptosis, which is crucial for maintenance of normal intestinal morphology [43]. Therefore, combined with the results of intestinal morphology, we suggest that FA supplementation during pregnancy might affect the degree of intestinal development by changing the apoptosis in the intestinal epithelial cells.
The small intestine is an important organ involved in the digestion of nutrients and its function can be used to evaluate its developmental quality [44]. D-glucose mainly relies on two kinds of transporters in intestinal mucosal epithelial cells the sodium/glucose cotransporter (SGLT) family and the glucose transporter (GLUT) family-to enter the tissues of the body, which is the main energy substance of the body. Glucose absorption in the intestinal tract mainly occurs in the anterior segment of the small intestine, so the expression of SGLT family genes in the small intestine is extremely important for the ability to absorb glucose [45]. Moreover, the maternal nutrition during gestation could affect the concentration of intestinal enzymes in the offspring [46]. Lactase (LCT), also known as β-galactosidase, is an enzyme, which under normal circumstances catalyzes the hydrolysis of lactose into galactose and glucose. In most mammals, the expression and catalytic activity of LCT reached the highest level at birth, decreased significantly after weaning, and the expression in the jejunum was the highest [47]. In this study, dietary FA supplementation in gestation generally increased the expression of SGLT1 and LCT, suggesting that FA may be conducive to lactose hydrolysis and glucose absorption, further supplying energy for the body. In addition, the small intestinal disaccharidase (SI) plays an important role in the utilization of carbohydrates in newborn animals, as disaccharides cannot be directly absorbed and utilized by the small intestine. The SLC2A5 gene, also known as GLUT5, is involved in encoding a fructose transporter [48]. We have found that the genes expression of SI and SLC2A5 significantly increased in triplets compared with that in twins, which is consistent with the result of IGF-I expression in jejunum tissue, and consistent with what we found in terms of small-intestine-development-related gene expression. In this case, the increased expression of digestion-related genes in triplets could actually be compensation for the smaller absorptive surface area in the small intestine.
The relationship between small intestinal morphology and gene expression was investigated using a Pearson correlation analysis. Multiple development-and digestion-related genes were positively correlated with crypt depth in the small intestine, which represents the degree of intestinal development because it provides a continuous flow of epithelial cells to the intestinal villi. Taken together, these findings suggested that FA may improve intestinal growth by influencing the expression of some genes that regulate crypt development. We also found that in duodenum and jejunum tissue, most of the digestion-related genes had a nonsignificant negative correlation with the muscle layer thickness. The decrease of the thickness of the muscle layer of the intestine could improve the digestion and absorption of nutrients [49]. In addition, the duodenal villi height was insignificantly positively correlated with the expression of most genes, but not in the jejunum tissue; a possible explanation for this may be due to the tissue specificity of gene expression.

Conclusions
In conclusion, this study found that FA supplementation during gestation could improve the morphological development of duodena and jejuna, the expression of development-, antiapoptotic-and digestion-related genes, as well as reduce the expression of proapoptotic-related genes in newborn lambs. Meanwhile, litter size affected the growth of the small intestine in offspring as the phenotypic development in twins was better, while the expression level of genes in triplets was higher. The results indicate that FA, to a certain extent (at the level of gene expression), could make up the perturbed development caused by maternal nutritional deficiency in triplet lambs, but not phenotypically. As for which F16 or F32 has the better effect, it cannot be judged based on the existing data and deserve future study.