Identification and Cloning of a CC-NBS-NBS-LRR Gene as a Candidate of Pm40 by Integrated Analysis of Both the Available Transcriptional Data and Published Linkage Mapping
Abstract
:1. Introduction
2. Results
2.1. Identification of Pm40 Effectiveness against Bgt Infection
2.2. Functional Annotation of the Genes within the Region Flanked by BF478514 and BF291338
2.3. Polymorphic EST Sequences in the Pm40 Region
2.4. Differential Expression Level of Genes within Pm40 Candidate Region
2.5. Screening and Cloning of TraesCS7B01G164000 and TraesCS7B01G164600
2.6. Validation of the Candidate Gene by Cosegregation with Powdery Mildew Resistance
2.7. Expression Analysis of TraesCS7B01G164000 after Bgt Inoculation by qRT-PCR
3. Discussion
3.1. The Broad Spectrum and Putatively Durable Resistance of Pm40
3.2. The Possible Chromosomal Regions Containing the Candidate Pm40 Gene
3.3. TraesCS7B01G164000 as the Putative Candidate Gene of Pm40
3.4. Validation of TraesCS7B01G164000 as the Candidate Gene of Pm40
4. Materials and Methods
4.1. Plant Materials, the Resistance Evaluations, and the Cytological Observations of Cell Death
4.2. Annotation of the Genes within the Physical Region of Pm40
4.3. Acquisition of RNA Sequencing Data and Detection of Deferentially Expressed Gene
4.4. Variation Extraction, Filtering and Annotation
4.5. Gene Cloning and Sequence Analysis
4.6. SNP Genotyping by AS-PCR
4.7. Gene Expression Analysis by qRT-PCR
Supplementary Materials
Author Contributions
Funding
Informed Consent Statement
Acknowledgments
Conflicts of Interest
References
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Gene ID | Pfam Annotation | Swissprot Annotation | Eggnog Annotation | NR Annotation |
---|---|---|---|---|
TraesCS7B01G146500 | Protein tyrosine kinase | Serine/threonine-protein kinase | Signal transduction mechanisms | Serine/threonine-protein kinase |
TraesCS7B01G148500 | Protein tyrosine kinase | Wall-associated receptor kinase | Signal transduction mechanisms | Wall-associated receptor kinase |
TraesCS7B01G148600 | Protein tyrosine kinase | Wall-associated receptor kinase | Signal transduction mechanisms | Wall-associated receptor kinase |
TraesCS7B01G148700 | Protein tyrosine kinase | Wall-associated receptor kinase | Signal transduction mechanisms | Wall-associated receptor kinase |
TraesCS7B01G148800 | Protein tyrosine kinase | Wall-associated receptor kinase | Signal transduction mechanisms | Wall-associated receptor kinase |
TraesCS7B01G162500 | Protein tyrosine kinase | Chitin elicitor receptor kinase | Signal transduction mechanisms | predicted protein |
TraesCS7B01G164000 | NB-ARC domain | Putative disease resistance protein | Signal transduction mechanisms | resistance protein RGA2 |
TraesCS7B01G164600 | Protein tyrosine kinase | PTI1-like tyrosine-protein kinase | Signal transduction mechanisms | PTI1-like tyrosine-protein kinase |
Gene ID | Variants_Impact_HIGH | Variants_Impact_MODERATE | |||
---|---|---|---|---|---|
Frameshift | Splice Acceptor | Splice Donor | Stop Gained | Missense Variant | |
TraesCS7B01G146300 | 0 | 0 | 0 | 0 | 3 |
TraesCS7B01G149500 | 0 | 3 | 0 | 0 | 2 |
TraesCS7B01G150600 | 0 | 0 | 6 | 0 | 2 |
TraesCS7B01G150900 | 2 | 0 | 0 | 0 | 0 |
TraesCS7B01G151800 | 0 | 0 | 0 | 0 | 3 |
TraesCS7B01G152800 | 0 | 0 | 0 | 2 | 0 |
TraesCS7B01G153200 | 0 | 0 | 0 | 0 | 1 |
TraesCS7B01G155400 | 0 | 0 | 0 | 0 | 3 |
TraesCS7B01G155600 | 0 | 0 | 0 | 0 | 1 |
TraesCS7B01G157800 | 0 | 0 | 0 | 0 | 2 |
TraesCS7B01G159900 | 0 | 0 | 0 | 0 | 1 |
TraesCS7B01G160100 | 0 | 0 | 0 | 0 | 2 |
TraesCS7B01G160200 | 0 | 0 | 0 | 0 | 2 |
TraesCS7B01G160700 | 0 | 0 | 0 | 0 | 1 |
TraesCS7B01G160800 | 1 | 0 | 0 | 0 | 3 |
TraesCS7B01G160900 | 0 | 0 | 0 | 0 | 1 |
TraesCS7B01G161200 | 0 | 0 | 0 | 0 | 5 |
TraesCS7B01G161300 | 0 | 0 | 0 | 0 | 4 |
TraesCS7B01G161400 | 1 | 0 | 0 | 0 | 2 |
TraesCS7B01G161500 | 0 | 0 | 0 | 0 | 2 |
TraesCS7B01G161600 | 0 | 0 | 0 | 0 | 2 |
TraesCS7B01G162300 | 2 | 0 | 0 | 0 | 1 |
TraesCS7B01G162500 | 0 | 0 | 0 | 0 | 3 |
TraesCS7B01G162600 | 0 | 0 | 0 | 0 | 1 |
TraesCS7B01G162700 | 0 | 0 | 0 | 0 | 5 |
TraesCS7B01G162900 | 0 | 0 | 0 | 0 | 1 |
TraesCS7B01G163000 | 0 | 0 | 0 | 0 | 1 |
TraesCS7B01G163400 | 0 | 0 | 0 | 1 | 0 |
TraesCS7B01G163500 | 0 | 0 | 0 | 0 | 1 |
TraesCS7B01G163800 | 0 | 0 | 0 | 0 | 2 |
TraesCS7B01G164000 | 0 | 0 | 0 | 0 | 2 |
TraesCS7B01G164100 | 0 | 1 | 0 | 0 | 0 |
TraesCS7B01G164200 | 0 | 0 | 0 | 0 | 1 |
TraesCS7B01G164500 | 0 | 0 | 0 | 0 | 7 |
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Yang, H.; Zhong, S.; Chen, C.; Yang, H.; Chen, W.; Tan, F.; Zhang, M.; Chen, W.; Ren, T.; Li, Z.; et al. Identification and Cloning of a CC-NBS-NBS-LRR Gene as a Candidate of Pm40 by Integrated Analysis of Both the Available Transcriptional Data and Published Linkage Mapping. Int. J. Mol. Sci. 2021, 22, 10239. https://doi.org/10.3390/ijms221910239
Yang H, Zhong S, Chen C, Yang H, Chen W, Tan F, Zhang M, Chen W, Ren T, Li Z, et al. Identification and Cloning of a CC-NBS-NBS-LRR Gene as a Candidate of Pm40 by Integrated Analysis of Both the Available Transcriptional Data and Published Linkage Mapping. International Journal of Molecular Sciences. 2021; 22(19):10239. https://doi.org/10.3390/ijms221910239
Chicago/Turabian StyleYang, Huai, Shengfu Zhong, Chen Chen, Hao Yang, Wei Chen, Feiquan Tan, Min Zhang, Wanquan Chen, Tianheng Ren, Zhi Li, and et al. 2021. "Identification and Cloning of a CC-NBS-NBS-LRR Gene as a Candidate of Pm40 by Integrated Analysis of Both the Available Transcriptional Data and Published Linkage Mapping" International Journal of Molecular Sciences 22, no. 19: 10239. https://doi.org/10.3390/ijms221910239