Comparative Physiological Analysis of Methyl Jasmonate in the Delay of Postharvest Physiological Deterioration and Cell Oxidative Damage in Cassava
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Materials and Treatments
2.2. Visual PPD Evaluation
2.3. Assays of ROS Accumulation and Antioxidant Enzyme Activities
2.4. RNA Isolation and Quantitative Real-Time PCR (qRT-PCR)
2.5. Determination of Endogenous Melatonin and GA
2.6. Quantification of Starch, Soluble Sugar, Ascorbic Acid, and Carotenoid
2.7. Statistical Analysis
3. Results
3.1. The Effect of MeJA Treatment on PPD
3.2. MeJA Alleviates Cell Oxidative Damage through Modulation of ROS and Underlying Antioxidant Enzymes
3.3. MeJA Positively Modulates the Quality of Cassava Root Slices
3.4. MeJA Treatment Affects the Endogenous GA Content
3.5. The Effect of MeJA on the Expression of Melatonin Biosynthesis Genes and Melatonin Level
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Liu, G.; Li, B.; Li, X.; Wei, Y.; Liu, D.; Shi, H. Comparative Physiological Analysis of Methyl Jasmonate in the Delay of Postharvest Physiological Deterioration and Cell Oxidative Damage in Cassava. Biomolecules 2019, 9, 451. https://doi.org/10.3390/biom9090451
Liu G, Li B, Li X, Wei Y, Liu D, Shi H. Comparative Physiological Analysis of Methyl Jasmonate in the Delay of Postharvest Physiological Deterioration and Cell Oxidative Damage in Cassava. Biomolecules. 2019; 9(9):451. https://doi.org/10.3390/biom9090451
Chicago/Turabian StyleLiu, Guoyin, Bing Li, Xiuqiong Li, Yunxie Wei, Debing Liu, and Haitao Shi. 2019. "Comparative Physiological Analysis of Methyl Jasmonate in the Delay of Postharvest Physiological Deterioration and Cell Oxidative Damage in Cassava" Biomolecules 9, no. 9: 451. https://doi.org/10.3390/biom9090451