Role of naringin in the treatment of atherosclerosis

Atherosclerosis (AS) is a major pathological basis of coronary heart disease. However, the currently available medications are unable to effectively reduce the incidence of cardiovascular events in the majority of patients with AS. Therefore, naringin has been attracting considerable attention owing to its anti-AS effects. Naringin can inhibit the growth, proliferation, invasion, and migration of vascular smooth muscle cells, ameliorate endothelial cell inflammation and apoptosis, lower blood pressure, halt the cell cycle at the G1 phase, and impede growth via its antioxidant and free radical scavenging effects. These activities suggest the potential anti-AS effects of naringin. In this review article, we comprehensively summarized the latest findings on the anti-AS effects of naringin and their underlying mechanisms, providing a crucial reference for future research on the anti-AS potential of this agent.


Introduction
Atherosclerosis (AS) is a chronic inflammatory cardiovascular disease characterized by lipid deposition in the vascular wall and immune cell recruitment.This condition is a major risk factor for chronic heart disease (Liu et al., 2023).According to World Health Organization data, coronary heart disease accounts for seven million deaths worldwide annually (Liu et al., 2023).The main strategies for the prevention and treatment of arteriosclerosis include a balanced diet (Przybylska and Tokarczyk, 2022), body mass management (Hwang et al., 2023), smoking cessation and limited consumption of alcohol, regular physical activity (Xu et al., 2022), control of blood pressure, regulation of serum sugar and blood lipid levels, and anti-platelet therapy (Virani et al., 2023).However, the use of currently available medication can effectively reduce cardiovascular events in only 40% of patients with AS (Parsamanesh et al., 2019).An increasing number of studies focus on traditional Chinese medicine and its active ingredients due to their lipid-lowering and anti-AS effects.
Naringin is a flavonoid abundantly found in fruits and vegetables (particularly grapefruit and tomatoes) (Memariani et al., 2021;Viswanatha et al., 2017), which exists in two forms (i.e., naringenin and naringin).Naringenin is formed through rapid glycosylation of naringin by the liver enzyme naringinase.It has been established that the sour and bitter taste of citrus fruits is attributed to naringin rather than naringenin.Most clinical studies have shown that the main pharmacological effects of grapefruit are produced by naringin (Adetunji et al., 2023;Bailey et al., 2007).Moreover, evidence indicates that, in addition to the liver enzyme naringinase, intestinal bacteria possess the capability to hydrolyze naringin into naringenin.This process has the potential to generate hypoglycemic and hypolipidemic effects (Xulu and Oroma Owira, 2012).Due to the inhibitory effects of naringin on liver enzymes (cytochrome P450 enzymes), the consumption of grapefruit juice can reduce or increase the concentration of drugs that are metabolized in the liver, thereby altering their pharmacokinetics and potentially leading to toxicity (Heidary Moghaddam et al., 2020).Naringin exhibits various pharmacological activities, namely, antioxidant (Balachandran et al., 2023), antiinflammatory (Zhang et al., 2022), anti-apoptotic (Zhao et al., 2020), anti-ulcer (Wang et al., 2023), and anti-osteoporotic (Pi et al., 2023), among others.Thus, increasing attention has been focused on the anti-AS effects of naringin.Naringin inhibits vascular smooth muscle cell (VSMC) proliferation (Lee et al., 2009) and ameliorates endothelial cell inflammation and apoptosis (Zhao et al., 2020) by exerting antioxidant effects and scavenging free radicals (Zhang et al., 2022;Amini et al., 2022).In this review, we summarized the latest available information on the anti-AS effects of naringin and their underlying mechanisms, providing a reference for future research on the anti-atherosclerotic properties of naringin.
Hypertension (Global Cardiovascular Risk et al., 2023), dyslipidemia (Global Cardiovascular Risk et al., 2023;Capra et al., 2023), endothelial cell injury (Li et al., 2014;Li et al., 2017), and smooth muscle proliferation are pivotal factors in the pathogenesis of AS (Feng and Xu, 2023).Naringin exerts antiatherosclerotic effects by intervening in hypertension, ameliorating lipid disorders, safeguarding against endothelial cell damage, inhibiting smooth muscle proliferation, regulating the cell cycle, and modulating other mechanisms.The underlying mechanism may involve antioxidation, anti-inflammation, regulation of autophagy, and modulation of other pathways.

Naringin prevents AS by lowering blood pressure
Hypertension is associated with up to 13.5% of all deaths annually worldwide, and is the leading risk factor for cardiovascular disease, including AS (Global Cardiovascular Risk et al., 2023).Strict control of blood pressure significantly decreases the rates of cardiovascular events and all-cause mortality (Yntema et al., 2023;Virani et al., 2021).Findings have demonstrated the significant blood pressure-lowering effects of naringin in hypertensive rats with renal artery occlusion, potentially through the inhibition of oxidative stress (Visnagri et al., 2015).Additionally, naringin reduces systolic blood pressure in rats fed a highcarbohydrate and -fat diet, and improves both vascular and ventricular diastolic dysfunction (Alam et al., 2013).Its antihypertensive effects may be associated with the reduction of inflammatory cell infiltration, oxidative stress, plasma lipid concentrations, and improvement of hepatic mitochondrial function in rats (Alam et al., 2013).In another study, naringenin (a metabolite of naringin) significantly decreased cerebral glutathione S-transferase (GST) and superoxide dismutase (SOD) activity as well as the levels of glutathione (GSH) in rats with hypertension induced by oral administration of L-N G -Nitro arginine methyl ester (L-NAME) (Oyagbemi et al., 2020).Moreover, it restored the activity of renal catalase, SOD, GST, glutathione peroxidase (GPX), and glutathione reductase (GSR).Additionally, naringenin significantly decreased the expression of renal angiotensin-converting enzyme (ACE) and mineralocorticoid receptor (MCR) (Oyagbemi et al., 2020).These observations indicate that the hypotensive effect of naringenin is mediated by modulation of the MCR/ACE/kidney injury molecule 1 (MCR/ACE/KIM1) signaling pathway, and by its antioxidative properties (Oyagbemi et al., 2020).Other studies have demonstrated that naringin reduces angiotensin II receptor type 2 (AT2R) mRNA expression in the medulla and the ratio of AT1R/AT2R, while increasing ACE expression and the ACE/ACE2 ratio in the cortex and medulla.These findings propose a potential mechanism underlying the beneficial effects of naringenin on hypertensive nephropathy (Wang et al., 2019), as it restores the ACE/ACE2 imbalance and normalizes the renal AGTR1/AGTR2 protein ratio in hypertensive rats (Wang et al., 2019).However, contrasting evidence from the two-kidney, one-clip (2K1C) hypertension rat model suggests that naringenin does not affect blood pressure.This indicates that its antihypertensive effects might be specific to different models of hypertension (Wang et al., 2019) (Figure 1; Table 1).Naringin has demonstrated efficacy in reducing blood pressure in hypertensive rats with renal artery occlusion-induced renovascular dysfunction (Visnagri et al., 2015) and in rats fed a high-carbohydrate and highfat diet (Alam et al., 2013).However, in two-kidney one-clip (2K1C) (Wang et al., 2019) and NG-nitro-L-arginine methyl ester (L-NAME)-induced hypertensive left ventricular hypertrophy models (Gao et al., 2018), naringin was found to reduce ACE 1 expression; however, it did not significantly affect blood pressure.We had initially attributed the inconsistent impact of naringin on blood pressure in hypertensive animal models to variations in dosage and administration methods.However, a detailed review of the literature showed that all four modes of administration involved intra-intestinal delivery with similar dose ranges.This controlled for the potential influence of drug dosage and administration routes on the antihypertensive efficacy of naringin.We believe that the inconsistent effects of naringin on blood pressure in hypertensive rats may be attributed to the pathogenesis of hypertension and factors related to the animal model.In rats with L-NAME-induced hypertension, a reduction in nitric oxide (NO) is primarily involved; the mechanism underlying hypertension induced by both the 2K1C model and renal artery obstruction appears similar.However, the degree of renal artery occlusion may vary.In both 2K1C and renal artery obstruction rat models, naringin decreased ACE1 expression; this was possibly caused by inconsistent reductions in ACE1 levels within each model.Blood pressure therefore remained unaffected despite an improvement in the kidney lesions.The effects of naringin on blood pressure in hypertensive rat models warrant further investigation.

Naringin prevents AS by ameliorating dyslipidemia
Dyslipidemia is widely recognized as an important risk factor associated with the morbidity and mortality caused by AS (Yu et al., 2022;Hong et al., 2022;Capra et al., 2023).Dyslipidemia is a metabolic disorder characterized by elevated levels of serum cholesterol, triglycerides, low-density lipoprotein, and very-lowdensity lipoprotein, as well as decreased levels of high-density lipoprotein cholesterol (Hong et al., 2022).Increasing evidence suggests that naringin exerts inhibitory effects on the progression of AS by ameliorating dyslipidemia (Pu et al., 2012).Naringin attenuates foam cell infiltration into plaques and reduces lesion areas in the aortic sinus (Li et al., 2014).Intragastric administration of naringin significantly reduced total cholesterol and triglyceride levels, while increasing high-density lipoprotein cholesterol levels in mice fed a high-fat diet (Yu et al., 2022).In type I diabetic rats, naringin did not significantly alter hyperglycemia; however, it effectively improved atherogenic dyslipidemia by increasing the levels of high-density lipoprotein cholesterol (Xulu and Oroma Owira, 2012).Moreover, in rabbits fed a 0.25% cholesterol diet, naringin reduced serum cholesterol levels and demonstrated remarkable efficacy in reducing the area of fatty streaks in the thoracic aorta and subintimal foam cell infiltration (Choe et al., 2001).Additionally, naringin inhibited the hypercholesterolemiainduced expression of intercellular adhesion molecule 1 (ICAM1) on endothelial cells, suggesting that the suppression of ICAM1 contributes to its anti-atherogenic effect (Choe et al., 2001).
Non-target metabolomics analysis showed that naringin modulates the hepatic levels of cholesterol derivatives and bile acids (Wang F. et al., 2020) by inhibiting 3-hydroxy-3methylglutaryl-CoA reductase (HMGCR) and acyl-coenzyme cholesterol acyltransferase (ACCA), respectively.Further investigation suggested that the gut microbiota-liver-cholesterol axis may represent the primary potential pathway through which naringin exerts its anti-atherosclerotic effects.Naringin alters the abundance of bacteria producing bile salt hydrolase and 7αdehydroxylase, thereby promoting bile acid synthesis from cholesterol.This is achieved by upregulating cholesterol 7αhydroxylase (cytochrome P450 family 7 subfamily A member 1 [CYP7A1]) expression via suppression of the farnesoid X receptor/fibroblast growth factor 15 (FXR/FGF15) pathway (Wang F. et al., 2020).Through oral administration, naringin predominantly localizes in the intestine due to the high solubility of 7-O-nohesperidoside.It exerts its anti-atherosclerotic effects primarily by augmenting bile acid synthesis through modulation of the gut microbiota-FXR/FGF15-CYP7A1 pathway (Wang F. et al., 2020).Naringin modulates the composition of gut microbiota by reducing the relative abundance of Bacteroides, Bifidobacterium, and Clostridium genera, which are associated with bile acid metabolism, while increasing that of Eubacterium genus that possesses bile acidhydrolyzing activity and reduces the levels of bile acids (Wang F. et al., 2020).The administration of naringin facilitates the accumulation of conjugated bile acids, including tauro-α/βmuricholic acid, while concurrently inhibiting the expression of FGF15 through the inactivation of FXR.The expression of CYP7A1, which encodes the rate-limiting enzyme in the biosynthesis of bile acids from cholesterol, was enhanced by downregulation of the FXR/ FGF pathway (Wang F. et al., 2020).Modulation of gut microbiota remodeling by naringin (a natural polyphenolic compound) has a profound impact on cholesterol metabolism and AS.In addition, recent findings have shown that naringin supplementation increases the abundance of Bifidobacterium and Lachnospiraceae bacterium 28-4, while reducing that of Lachnospiraceae bacterium DW59 and Dubosiella newyorkensis.Naringin supplementation has also been found to alter the fecal metabolite profile by significantly promoting the production of taurine, tyrosol, and thymol, which offers benefits in atherosclerosis by reducing oxidative stress, inflammation, hyperlipidemia, and lipid lesions in the aortic intima (Zoubdane et al., 2024;Yu et al., 2016).
In addition, naringin suppressed the expression of proprotein convertase subtilisin/kexin type 9 (PCSK9) and an inducible degrader of low-density lipoprotein receptor (IDOL), thereby promoting reverse cholesterol transport to the liver from peripheral tissues.The excretion of cholesterol from the liver to the gallbladder was also enhanced, as was that via the enterohepatic circulation.These mechanisms collectively decrease the cholesterol levels, thus alleviating AS.Notably, the expression of genes in the FXR/FGF15 pathway, particularly CYP7A1, was significantly inhibited by gut microbiota remodeling.Therefore, modulation of cholesterol metabolism by gut microbiota remodeling may be implicated in the effect of naringin on AS (Figure 2; Table 1).
Furthermore, naringin promotes reverse cholesterol transport by downregulating the expression of PCSK9/IDOL (Wang F. et al., 2020).Clinical investigations revealed that naringin significantly reduces the body mass index, total cholesterol, and low-density lipoprotein cholesterol, whereas it concurrently elevates the levels of adiponectin in adult patients diagnosed with dyslipidemia.These findings suggest that naringin holds potential as a therapeutic agent Naringin decreased sheer stress injury by lowering blood pressure; this effect was mediated by the attenuation of MCR/KIM1, and normalization of AT1R/AT2R and ACE/ACE2 ratios.Naringin is rapidly metabolized by the liver enzyme naringinase to produce naringenin.Naringenin decreased blood pressure and reduced vascular shear stress injury.Consequently, it decreased the occurrence of tissue atherosclerosis by adjusting the MCR/KIM1, AT1R/ AT2R, and ACE/ACE2 ratios, and increasing the levels of GST, SOD, and GSH.Abbreviations: AT1R, angiotensin II type 1 receptor; AT2R, angiotensin II receptor type 2; ACE: angiotensin converting enzyme; GSH, reduced glutathione; GST, glutathione S-transferase; KIM, kidney injury molecule; MCR, mineralocorticoid receptor; SOD, superoxide dismutase.in the management of metabolic disorders (Barajas-Vega et al., 2022).The findings were further validated by the inclusion of bergamot juice extract, which contains naringin as one of its primary bioactive constituents.Supplementation with this extract has been shown to effectively lower blood lipid levels, and thereby offer potential benefits for individuals with dyslipidemia (Toth et al., 2015).

Naringin attenuates progression of AS by safeguarding endothelial dysfunction
Naringin enhances the survival rate of human umbilical vein endothelial cells (HUVECs) and preserves the functional and structural integrity of the endothelium, which plays a crucial role in inhibiting the advancement of AS.Thus, the preventive effect of naringin on endothelial dysfunction could potentially delay the progression of AS (Li et al., 2014).Pretreatment with naringin attenuates endothelial inflammation, reduces oxidative stress, enhances NO release, and inhibits the degradation of zona occludens 2 (ZO-2), occludin (OCLN), and vascular endothelialcadherin.These effects preserve the functional and structural integrity of the endothelium (Zhao and Zhao, 2022).It has been demonstrated that the therapeutic effects of naringin are mediated through the inhibition of trimethylamine-N-oxide (TMAO)-stimulated mitogen-activated protein kinase (MAPK) signaling in HUVECs (Zhao and Zhao, 2022).In another investigation, naringin augmented the survival rate of HUVECs and mitigated the elevations in the levels of reactive oxygen species (ROS) and intracellular Ca 2+ induced by lipopolysaccharide in comparison to the control group (Bi et al., 2016).Moreover, naringin impedes cytochrome C release from mitochondria to cytosol, notably represses the protein or mRNA expression of interleukin 1 (IL1), IL6, tumor necrosis factor-alpha (TNF-α), vascular cell adhesion molecule 1 (VCAM1), ICAM1, nuclear factor-kappa B (NF-κB), activator protein-1 (AP-1), cleaved caspase 3/7/9 (CASP3/7/9), p53, and BCL2 associated X (BAX), and enhances the expression of BCL-extra large (BCL-xl) and BCL2 to curtail inflammation and apoptosis (Zhao and Zhao, 2022).Additionally, naringin robustly inhibits the phosphorylation levels of JUN N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and p38 MAPK (Bi et al., 2016), and reduces the expression

Reference Mode Main opinion
it reduced the mitochondrial membrane potential and the activities of CASP3 and CASP9.Naringin also potently inhibited EDN1, while enhancing NO synthesis.Assessment of the distal femoral microvascular density revealed that the naringin treatment group exhibited a significantly higher number of microvessels compared with the ovariectomized group, as well as a positive correlation between microvascular density and bone mineral density.  of cell adhesion molecules (VCAM1, ICAM1, and selectin E [SELE]), which are associated with the adhesion of THP-1 monocytes to HUVECs induced by TNF-α (Li et al., 2014).In addition, naringin markedly reduced the mRNA and protein levels of chemokines, such as fractalkine/C-X3-C motif chemokine ligand 1 (fractalkine/CX3CL1), and monocyte chemoattractant protein-1 (MCP-1), and regulated the activation of normal T cell expressed and secreted, induced by TNF-α (Hsueh et al., 2016).This effect is attributed to the potent inhibition of TNF-α-induced nuclear translocation of NF-κB by naringin, which is mediated by the suppression of IκB kinase α/β (IKKα/β), IκB-α, and NF-κB phosphorylation (Hsueh et al., 2016).These findings indicate that naringin exerts an anti-atherosclerotic effect by modulating the expression of cell adhesion molecules and chemokines.This is achieved by inhibiting the activation of the IKK/NF-κB signaling pathway induced by TNF-α (Hsueh et al., 2016).Naringin inhibits the production of ROS and the overexpression of NADPH oxidase 4 (NOX4) and p22 phox induced by TNF-α.Furthermore, it suppresses the activation of the NF-κB and phosphatidylinositol 3 kinase/protein kinase B (PI3K/AKT) signaling pathways.These findings suggest that naringin exerts preventive effects on HUVEC injury induced by alleviated oxidative stress and the inflammatory response.The potential mechanisms underlying these effects may involve inhibition of the NOX4 and NF-κB pathways, as well as activation of the PI3K/AKT pathway (Wang K. et al., 2020).Naringin inhibits the expression of vascular endothelial growth factor (VEGF), C-reactive protein (CRP), JNK2, p38, and NO, indicating that it reduces CRP expression, curtails the activity of JNK2 and p38 kinases, and represses the MAPK pathway.This leads to a decline in NO synthesis, VEGF levels, and endothelial adhesion factor expression (Pengnet et al., 2019).
These observations suggest that naringin regulates the inactivation of NO and shields endothelial function from ROS through its potent antioxidant properties (Ikemura et al., 2012).Naringin also significantly reduced serum starvation-induced apoptosis in endothelial cells (Shangguan et al., 2017).Moreover, it promotes the proliferation of lung vascular endothelial cells in newborn rats, downregulates the expression of glucose-regulated protein, 78 kDa (GRP78), C/EBP homologous protein (CHOP), CASP12, and cytochrome C proteins, reduces the mitochondrial membrane potential, and decreases the activities of CASP3 and CASP9 (Shangguan et al., 2017).In addition, naringin exhibits dual functionality in vitro and in vivo by suppressing endothelin and enhancing NO synthesis.In summary, naringin exerts its antiapoptotic effect on vascular endothelial cells by inhibiting the endoplasmic reticulum stress-and mitochondria-mediated pathways, thereby regulating endothelial cell function.Furthermore, naringin promotes angiogenesis, thus exerting its anti-osteoporotic effect (Shangguan et al., 2017) (Figure 3; Table 1).
5 Naringin inhibits the growth, proliferation, invasion, and migration of VSMCs, and induces cell cycle arrest at the G1 phase, demonstrating its potential utility in the prevention of AS Naringin exerts a significant inhibitory effect on cell growth and triggers VSMCs in response to TNF-α.The inhibitory mechanisms of naringin on TNF-α-induced VSMC proliferation, invasion, and migration might be mediated by the downregulation of matrix metalloproteinase 9 (MMP9) and AKT phosphorylation, which acts as a downstream effector of PI3K (Lee et al., 2009).Besides, treatment with naringin reduced TNF-α-induced MMP9 secretion by decreasing the binding of transcription factors NF-kB and AP-1 to DNA (Lee et al., 2009).Naringin also inhibited TNF-α-induced AKT, mechanistic target of rapamycin kinase (mTOR), and p70S6K phosphorylation (Lee et al., 2009).These results corroborate the notion that naringin inhibits the PI3K/AKT/mTOR/p70S6K pathway (Lee et al., 2009), which could potentially explain the mechanisms underlying the inhibitory effects of naringin on TNF-α induced proliferation, invasion, and migration of VSMCs.
It was demonstrated that treatment with naringin significantly inhibited cell growth and induced cell cycle arrest at the G1 phase.These effects were mediated by the induction of p21 wild-type p53 activated fragment-1 (WAF1) expression independent of p53.Furthermore, it was found that naringin downregulated the expression of cyclins (CCNs) and cyclin-dependent kinases (CDKs) in VSMCs (Lee et al., 2008;Li et al., 2014).Inhibition of ERK function promotes naringin-dependent p21WAF1 expression, counteracts naringin-mediated suppression of cell proliferation, and reduces the levels of cell cycle proteins.Concurrently, treatment with naringin augments the activation of both Ras and Raf.Transfection of cells with dominant negative Ras (RasN17) and Raf (RafS621A) mutant genes impedes naringin-induced ERK activity and p21WAF1 expression.Attenuation of naringin-induced reduction in cell proliferation and cell cycle proteins was observed in the presence of RasN17 and RafS621A mutant genes.The involvement of the Ras/Raf/ERK pathway in p21WAF1 induction was demonstrated, resulting in a decline of CCND1/CDK4 and CCNE/CDK2 complexes and naringin-dependent inhibition of cell growth (Lee et al., 2008).Naringin (10-25 µM) exhibits inhibitory effects on TNF-α-induced MMP9 expression in VSMCs, as well as on invasion and migration.Additionally, it mitigates the TNF-α-mediated release of IL6 and IL8.However, treatment of VSMCs with naringin in the presence of TNF-α does not affect cell growth and apoptosis.In additional experiments, naringin suppressed the transcriptional activity of AP-1 and NF-κB, which are two crucial nuclear transcription factors implicated in MMP9 expression.Moreover, treatment with naringin inhibited the PI3K/AKT/mTOR/p70S6K pathway in TNF-α-induced VSMCs.These results suggest that naringin antagonizes the PI3K/AKT/mTOR/ p70S6K pathway, thereby suppressing invasion and migration, and downregulating MMP9 expression through the transcription factors NF-κB and AP-1 in TNF-α-induced VSMCs (Lee et al., 2009) (Figure 4; Table 1).

Summary
In recent years, a growing body of evidence has corroborated the anti-atherosclerotic activity of naringin.These effects are attributable to its antioxidant capability, regulation of autophagy, amelioration of blood lipid disorders, inhibition of endothelial cell inflammation and vascular smooth muscle proliferation, reduction Mechanisms through which naringin inhibited the proliferation, invasion, and migration of VSMCs induced by TNF-α.The inhibitory effects of naringin on atherosclerosis were mediated by its ability to downregulate MMP9 and AKT phosphorylation (a downstream effector of PI3K), and reduce NF-κB activity, while also inhibiting the PI3K/AKT/mTOR/p70S6K axis.Naringin resulted in significant inhibition of growth and induced cell cycle arrest at the G1 phase by triggering p53-independent p21WAF1 induction, while concurrently downregulating the expression of cyclins (CCNs) and CDKs in VSMCs.In addition, blockage of ERK function repressed naringin-dependent p21WAF1 expression and concurrently enhanced the activation of Ras and Raf.Abbreviations: AKT, protein kinase B; AP-1, activator protein-1; CDKs, cyclin-dependent kinases; ERK, extracellular signal-regulated kinase; MMP, matrix metalloproteinases; mTOR, mammalian target of rapamycin; NF-κB, nuclear factor-κB; PI3K, phosphatidylinositol 3 kinase; VSMCs, vascular smooth muscle cells; WAF1, wild-type p53 activated fragment-1.
of the entry of low-density lipoprotein into macrophages, and suppression of foam cell formation (All views have been summarized in Table 1).However, most research has focused on fundamental inquiries, with relatively fewer clinical studies conducted thus far.Additional clinical trials are warranted to validate the therapeutic efficacy and potential adverse effects of naringin.In this context, the toxicity of naringin has been evaluated extensively; data from available studies have unequivocally demonstrated the toxic effects to be negligible.In their study, Li et al. (2014) found that naringin did not demonstrate any significant acute or chronic oral toxicity in SD rats.They also found the noobserved-adverse-effect-level in beagle dogs to be at least 500 mg/kg body weight per day, on oral administration for 3 and 6 consecutive months.These studies provide evidence of the favorable safety profile of naringin (Li et al., 2020).Such knowledge would assist in effectively utilizing naringin in clinical practice against AS.In its native state, naringin displays limited water solubility and weak efficacy.Thus, the application of nanoparticle formulation technology should be considered to alter the structure of naringin, with the aim to enhance its water solubility, bioavailability, and pharmacological activity (Dashputre et al., 2023).Additionally, a comprehensive assessment of its pharmacokinetic properties is crucial to fully investigate the potential benefits offered by this intriguing phytochemical.Frontiers in Pharmacology frontiersin.org11 Lu et al. 10.3389/fphar.2024.1451445

FIGURE 4
FIGURE 4 vascular cell adhesion molecule-1 VEGF vascular endothelial growth factor VSMC vascular smooth muscle cell

TABLE 1
Main opinions regarding the mode of action of naringin in the treatment of atherosclerosis.

TABLE 1 (
Continued) Main opinions regarding the mode of action of naringin in the treatment of atherosclerosis.

TABLE 1 (
Continued) Main opinions regarding the mode of action of naringin in the treatment of atherosclerosis.