Termite mound soil based potting media: a better approach towards sustainable agriculture

Termite mound soils are known to possess unique physico-chemical and biochemical properties, making them highly fertile. Considering their rich nutrient content, the objective of the current experiment is to assess the physico-chemical properties and enzyme activities of termite mound based potting media and evaluate theirperformance for further exploration in floriculture. Potting media consisting of termite mound soil (TS) of a subterranean termite, Odontotermes obesus were prepared in 7 different combinations with garden soil (GS), sand (S) and farmyard manure (FYM) and a control (without termite mound soil), i.e., T1 (TS, GS, S, FYM (v:v:v:v /1:2:1:1)), T2 (TS, GS, S, FYM (v:v:v:v / 2:1:1:1)), T3 (TS, S, FYM (v:v:v / 2:1:1)), T4 (TS, GS, FYM (v:v:v / 2:1:1)), T5 (TS, GS, S (v:v:v / 2:1:1)), T6 (TS, S, FYM (v:v:v / 3:1:1)), T7 (TS, S, FYM (v:v:v / 1:1:2)) and control (GS, S, FYM (v:v:v / 2:1:1)). The samples were then analysed in laboratory. Experimental analysis on physico-chemical and biological parameters revealed superiority of T7 (TS, S, FYM (v:v:v / 1:1:2)) in terms of pH (7.15), organic carbon (2.13%), available nitrogen (526.02 kg ha−1), available phosphorus (56.60 kg ha−1), available potassium (708.19 kg ha−1), dehydrogenase activity (18.21 μg TTF g−1 soil day−1), Phosphomonoesterase (PME) activity (46.68 54 μg p-nitrophenol/gsoil/h) and urease activity (3.39 μg NH4-N g−1 soil h−1). Whereas T4 (TS, GS, FYM (v:v:v /2:1:1)) registered superiority in terms of PME activity (50.54 μg p-nitrophenol/gsoil/h), Fluorescein diacetate (FDA) activity (11.01 μgfluorescein/gsoil/h) and Soil Microbial Biomass Carbon (SMBC) (262.25 μg/g). Subsequent to the laboratory analysis, two best potting mixtures (T7 & T4) were selected and their performance was assessed by growing a test crop, Tagetes erecta cv. Inca Orange. Considering the growth parameters, the potting media: T7 was found to be significantly superior in terms of plant spread (39.64 cm), leaf area index (4.07), fresh weight (37.72 g), yield (317.81 g/plant), and diameter (9.38 cm) of flower over T4 & control. The Benefit:Cost (B:C) ratio meaning the ratio of net returns to total cost of cultivation was determined. The B:C ratio of raising marigold flower as potted plant in T7 was 1.10 whereas the B:C ratio of the potting mixture of T7 was 2.52. This shows that T7 potting media is also economically viable choice for commercial purposes.


Introduction
Termite mounds are structures of soil built by termites in several tropical ecosystems (Jouquet et al., 2015).These biogenic structures are built to protect the colonies from various environmental and biological factors (Eze et al., 2020).These structures contain soil particles of clay and organic matter cemented together (Harit et al., 2017;Jouquet et al., 2021Jouquet et al., , 2022)).Subterranean mound-building termite species Odontotermesobesus (Rambur) are mostly found concentrated in tropical regions of the world, viz., Africa, Asia, etc. (Ashraf et al., 2020).It is the most abundant species with mound constructing ability to be found in Assam (Biswas and Deka, 2019).The type of soil and climatic conditions favour the population growth of termites (Bhattacharyya et al., 2014).Mounds of O. obesus are conical while some can be cathedral or lenticular as in South India (Harit and Jouquet, 2021).Despite termites being "silent destroyers" (Bhairavi et al., 2021), they are key soil bioturbators in the tropics (Cheik et al., 2022) and their constructed mounds are highly beneficial with rich nutrient content and beneficial microorganisms.Apart from these, termite soil also contains some useful plant growth promoting bacteria such as Bacillus sp., Citrobacter freundii, Azotobacter sp., and Pseudomonas sp., which are capable of solubilizing phosphate and potassium (Adebajo et al., 2021).Such beneficial properties and microbial activity present in termite mounds makes it ideal for use as a potting mixture.
Due to termite activity, the mounds have some unique physicochemical and biochemical properties as compared to the surrounding soils for e.g.pH, organic carbon, cation exchange capacity, NPK content, etc.The effects of termite mound soil on crop growth have been studied by many workers in paddy (Miyagawa et al., 2011), maize (Bama and Ravindran, 2018), and tomato (Garba et al., 2011), due to its ability to supply nutrients.Such experiments showed excellent growth effect of termite mound soil on aforementioned crops.But it has not been examined on floriculture till date.Moreover, its effect particularly as potting media had not been experimented.Hence, the present investigation has been credited as a maiden attempt from India to assess the effectiveness of termite mound soil as potting media.
Considering all the beneficial properties of termite mound soil, the aim of the current experiment is to investigate the influence of termite mound soil on conventional potting media by analyzing its physico-chemical and biological properties and then evaluate its effect on growth of floriculture plants like marigold.In India, marigold (Tagetes spp.) occupies about two-third of the total area under cultivation with a total production of 1,754,000 MT, from an area of 255,000 ha (Kaur et al., 2021).

Experimental site
The present experiment was carried out in the campus of Assam Agricultural University, Jorhat, Assam, India.It is situated in 26°72/N latitude and 94°20/ E longitude, at an altitude of 86.6 m above mean sea level.The mean annual rainfall is 2,375 mm while the maximum and minimum temperature are 33.9 and 9.7°C, respectively.The soil type is alluvial and vegetation includes wide variety from wild plant species to cultivated crops.

Experimental materials
The top portion of the soil of randomly selected five termite mounds of O. obesus was collected (10kgs) and mixed up thoroughly to prepare a composite sample.The collected soil was spread on plastic sheets and was exposed to sunlight for 2 days for the escape of live termites.The garden soil, sand and FYM was collected from the experimental site.All the components were then thoroughly mixed according to the treatments and samples were collected for laboratory analysis.High quality hybrid seeds of African marigold (Tagetes erecta) variety Inca Orange were procured from "Syngenta flowers" and plastic pots (30 cm diameter) were used to grow the test crop.In the present study, the cultivar 'Inca Orange' was selected as it has remarkable growth characteristics and is fully adapted to agro-climatic conditions of Assam.Additionally, it has bagged the Royal Horticultural Society's Award of Garden Merit.

Preparation of potting media
The potting media was prepared using termite mound soil, garden soil, sand and FYM (Farmyard manure), i.e., decomposed mixture of cow litter and urine in different proportions.

Processing of potting media for laboratory analysis
Potting media was prepared as per the treatment combinations and were dried, clods were broken down and the dried soil was sieved using a 2 mm sieve for laboratory analysis.Before drying, one part of each combination was left fresh and moist at 4°C without any processing to analyze the enzymatic properties of soil.

Analysis of potting media samples
Soil physico-chemical properties such as pH, electrical conductivity, water holding capacity, infiltration rate, organic carbon, available nitrogen (N), available phosphorus (P) and available potassium (K) and biological properties such as soil microbial biomass carbon (SMBC) and enzyme activities viz., dehydrogenase, phosphomonoesterase (PME), fluorescein diacetate (FDA), and urease were analysed.
The pH and EC, 1:2.5 were determined though soil water suspension method (Jackson, 1973) (Keen and Raczkowski, 1921).Techniques employed to measure the organic carbon, available N, P, and K include wet digestion method (Walkley and Black, 1934), alkaline permanganate method (Subbiah and Asija, 1956), Bray and Kurtz no.1 method (Jackson, 1973) and neutral N Ammonium acetate method (Jackson, 1973), respectively.For estimation of biological properties, SMBC was determined by chloroform fumigation extraction method (Jenkinson and Powlson, 1976).The enzymatic activity of dehydrogenase was estimated according the methodology described by Casida et al. (1964), PME was measured using p-nitrophenol phosphate (Tabatabai and Bremner, 1969) and the technique utilized for urease was non-buffer method using colorimetric determination of ammonium (Kandeler and Gerber, 1988).
Out of the seven treatments, two best treatments were selected from the laboratory analysis and the test crop was grown in pots in those best treatments.Best treatment, i.e., high organic carbon, high available nitrogen, phosphorus, potassium, pH around neutral and high biological activity.

Experimental design
The pot experiment was laid out in Completely Randomized Design (CRD) with 2 best treatments and a control with 8 replications under field conditions.
The seeds of the marigold variety Inca Orange were sown on 10-11-2022 and transplanted to the pots on 10-12-2022.Double pinching was performed.First pinching was carried out ten days after visibility of first flower bud and second pinching was done ten days after single pinching.

Analysis of morphological growth characteristics of the test crop
Morphological characters viz., vegetative and reproductive growth characteristics were measured during the mature flowering stage of the plant.Vegetative growth parameters were-plant height, plant spread, number of branches per plant, number of leaves per plant, leaf area index (LAI), stem diameter, number of roots per plant, root length and root diameter.The reproductive growth parameters included days to visibility of first flower bud, days to opening of flower, days to full bloom, flowering duration on plant, days from opening to fading of individual flower, flower bud diameter, flower diameter, number of flowers per plant, fresh weight of flower and yield of flower.
After harvesting of the crop at the end of the season, the potting media from the crop root zone was collected and again analysed for physico-chemical and biological properties to see the effect of crop on potting media.

Economics
The cost of cultivation was calculated out for each treatment per 100 pots.The labour and operational cost and all the prevailing inputs were taken into account while calculating the cost of cultivation.The value of products at the prevailing market price is the gross return while the net return is calculated by subtracting the cost of cultivation from gross return.The benefit cost (B:C) ratio was calculated by dividing the net return by cost of cultivation.The B:C ratio as a potted plant and the B:C ratio for potting media was calculated accordingly.

Statistical analysis
The physico-chemical and enzymatic activities of potting media were statistically analysed and the quantitative data pertaining to various growth characteristics of test crop were statistically analysed adopting the procedure of Analysis of Variance (ANOVA) by Panse and Sukhatme (1985).Whenever variance ratio was found significant, critical difference (CD) was calculated at 5 per cent level of significance, otherwise only Standard Error of Difference (S.Ed) (±) was mentioned.Pearson's correlation technique was used to measure the correlation among the physico-chemical and biological parameters of potting media.The analyses were carried out using version 21 of IBM SPSS.

Physico-chemical properties of potting media
The outcome of the physico-chemical properties presented in Table 1 showed that the pH of the potting media ranged from 5.14-8.03.T 3 (7.15),T 7 (7.15), and T 4 (6.95) were observed to be nearly neutral range ideal for growing the test crop.The highest EC (1.88 ds/m) and water holding capacity (32.46%) were observed in T 6 while the least value (1.45 ds/m) was recorded in T 1 and T 5 which were statistically at par with control (1.47 ds/m).Highest water holding capacity (32.46%) was reported in T 6 while the lowest (26.00%) was reported in control and the vice versa was observed in the infiltration rate.The infiltration rate was highest in control (9.39 mm/h) while lowest in T 6 (5.34 mm/h).The organic carbon content of the potting media ranged from 0.85 to 2.13% (Table 1).It was found to be significantly high in T 7 (2.13%) followed by T 4 (1.29%) which was statistically at par with T 6 (1.27%) while the lowest was recorded in T 5 (0.85%).According to the data, maximum amount of available nitrogen was recorded in T 7 (526.02kg ha −1 ) followed by T 4 (275.17kg ha −1 ) while the minimum amount was found in T 5 (162.97kg ha −1 ).Available phosphorus content was observed to be significantly highest in T 7 (56.60 kg ha −1 ) followed by T 4 (34.31kg ha −1 ) while lowest available phosphorus content was found to be in T 5 (22.07 kg ha −1 ).The highest available potassium content was observed in T 7 (708.19kg ha −1 ), followed by T 4 (621.54kg ha −1 ) while the lowest was observed in T 5 (308.25 kg ha −1 ) and control (308.25 kg ha −1 ).

Biological and enzymatic properties of potting media
The highest SMBC was recorded in T 4 (262.25 μg/g) followed by T 7 (235.01μg/g) while the lowest microbial biomass carbon was observed in T 5 (109.36μg/g) (Table 1).In case of dehydrogenase activity, it was revealed that T 7 had the highest dehydrogenase activity (18.21 μg TTF g −1 soil day −1 ) which was followed by T 4 (12.64 μg TTF g −1 soil day −1 ).However, the lowest dehydrogenase activity was observed in T 5 (2.64 μg TTF g −1 soil day −1 ).The highest PME activity was recorded in T 4 (50.54 μg p-nitrophenol/g soil/h) which was found to be statistically at par with T 7 (46.68 μg p-nitrophenol/g soil/h).The minimum PME activity was recorded in T 5 (29.17 μg p-nitrophenol/g soil/h).T 4 (11.01 μg fluorescein/g soil/h) had the highest amount of FDA activity which was followed by T 7 (8.18μg fluorescein/g soil/h).The lowest FDA activity was measured in T 5 (3.66 μg fluorescein/g soil/h).Lastly, the highest urease activity was observed in T 7 (3.39 μg NH 4 -N g −1 soil h −1 ) which was statistically at par with the control (3.19 μg NH 4 -N g −1 soil h −1 ).It was followed by T 4 (2.24 μg NH 4 -N g −1 soil h −1 ) and T 3 (2.20 μg NH 4 -N g −1 soil h −1 ) while the lowest was registered in T 5 (0.56 μg NH 4 -N g −1 soil h −1 ).
Thus, the physico-chemical and biological analysis of all the seven treatments revealed that both T 7 and T 4 were superior in terms of pH, organic carbon, available nitrogen, available phosphorus, available potassium, dehydrogenase, PME, urease activity and PME, FDA, SMBC, respectively.Therefore, these two treatments were selected for growing the test crop Tagetes erecta cv.Inca Orange.

Vegetative growth parameters of the test crop
Results showed no significant difference among the treatments in case of plant height (Table 3).The mean value recorded in T 7 , T 4 and

Reproductive growth parameters of the test crop
The number of days to visibility of first flower bud was found to be shortest in T 7 (40.13days) and T 4 (40.75 days) which were statistically at par (Table 3).Concurrently, the earliest opening of flower was observed in T 7 (55.75 days) and T 4 (57.13 days).The shortest time to full bloom was observed in T 7 (91.25 days) and T 4 (89.88 days) both being statistically at par.No significant difference was observed between T 7 (58.50days) and T 4 (58.50 days) regarding flowering duration.The highest number of days from opening to fading of individual flower was observed in T 7 (49.63 days) and T 4 (49.13 days) being statistically at par.The flower bud diameter was reported to be high in T 7 (0.74 cm) which was statistically at par with T 4 (0.71 cm).The largest diameter of flower was recorded in T 7 (9.38 cm) while the smallest flower diameter was observed in control (6.74 cm).The highest number of flowers were produced by control (33.13).Similarly, the maximum fresh flower weight and highest yield of marigold was recorded in T 7 at 37.72 gm and 317.81 gm/plant, respectively.

Impact of crop Tagetes erecta cv. Inca Orange on soil properties
At the end of the crop season, the crop was harvested and the potting media was tested to draw the impact of the crop on the potting media (Table 4).It is graphically depicted in Figures 1, 2. All the chemical properties viz., pH, EC, organic carbon, available nitrogen, available phosphorus and available potassium gradually decreased while all the biological properties viz., SMBC, dehydrogenase, FDA, PME and urease activity gradually increased after harvesting of the crop (Figures 3A-D).

Economics of Tagetes erecta cv. Inca Orange using termite mound based potting media
According to cost analysis (Table 5), the highest B:C ratio as a potted plant was observed in T 7 (1.10).Similarly, the B:C ratio as a potting media (Table 5) was recorded to be highest in T 7 (2.52).

Discussion
According to the data presented in Table 1, the pH values of the potting media were moderately acidic to moderately alkaline.Moderately acidic pH was observed only in T 5 because of the lack of organic matter.Typically, termite mound soils have an acidic pH which explains the slight acidity of the treatments (Li et al., 2017;Apori et al., 2020).However, some other workers had reported pH of the termite mound soil as alkaline (Dhembare, 2013;Subi and Sheela, 2020).Contrary to the above information, some studies have reported no significant difference in the pH of termite mound soil and its adjacent soil (Brossard et al., 2007).The EC depends on the textural class (Hamarashid et al., 2010) and on the mineralisation of organic matter and presence of micronutrients.Therefore, highest EC in T 6 was most likely due to highest proportion of termite mound soil.This result corroborates with the earlier work of Ibrahim et al. (2022) who reported higher EC in termite mound soil than the adjacent soil.The two major factors that affect the soil water holding capacity are soil organic matter and soil texture (Kartik and Jain, 2022).Fine textured soils like clay generally have a high water holding capacity as compared to other soil types.The composition of potting media in T 6 and T 4 registered higher organic matter and hence higher water holding capacity.The present study showed that the control, T 1 and T 5 had the lowest water holding capacity.Comparatively lower water holding capacities registered in the above treatments were probably because of low organic matter content of the potting mixtures .As a general fact, decaying plant material and termite excreta attributed to the high amount of organic carbon found in termite mound soils.A similar trend was reported by Bachha et al. (2022) who found 24.53 and 10.78 per cent water holding capacity in Odontotermes spp.mound soil and its surrounding soil, respectively, vividly indicating high water holding capacity in termite mounds.Infiltration rate determines how quickly water penetrates the soil and reaches the plant roots ensuring both water and nutrient availability to the crops.The potting media viz., T 1 and T 7 displayed relatively high infiltration rate which points to the availability of high amount of nitrogen, phosphorus and potassium.The present observations are in agreement with Adugna et al. (2016) and Ackerman et al. (2007) who noticed higher infiltration rate in termite mound than the adjacent soil due to termite foraging activity.Also, presence of FYM in enhancing soil infiltration rate was investigated by Zhang et al. (2014) who found that saturated hydraulic conductivity (a critical indicator of soil infiltration) had a significant effect due to application of FYM.The highest organic carbon was observed in T 7 which was followed by T 4 .These two treatments contained relatively higher proportion of termite mound soil including Impact of crop on physico-chemical parameters of potting media.

FIGURE 2
Impact of crop on biological parameters of potting media. 10.3389/fmicb.2024.1387434 Frontiers in Microbiology 08 frontiersin.orgFYM.Feeding of termites on cellulose along with its behaviour of gathering organic material for nesting purposes, promoting decomposition and nutrient cycling contributes greatly to high content of organic matter in soil.The possible reason for lowest organic carbon content as recorded in T 5 might be due to the non-inclusion of FYM.Similar conclusions were drawn by Dhembare (2014) and Chisanga et al. (2020), who found higher organic carbon in termite mound soil than surrounding soil.High organic matter acts as a reservoir of nitrogen.Hence, high nitrogen content is present in termite mounds (Jose and Maya, 2020).Highest organic carbon in T 7 can explain the high amount of available nitrogen in T 7 due to organic matter mineralization.Moreover, the bacteria present in termite hindguts have proved to be an important pathway for nitrogen fixation as reported by Mullins et al. (2021).The potting media T 5 recorded the lowest available nitrogen due to least amount of organic matter.Phosphorus availability is mostly affected by soil pH and organic matter.Highest available phosphorus recorded in T 7 (Table 2) might be because of appropriate pH (7.15) and high organic matter content.
In acid soils, phosphorus fixation takes place because of iron and aluminium oxides while in alkaline soils calcium and magnesium react with phosphorus which leads to phosphorus fixation.High available phosphorus in termite mounds as compared to surrounding soil was observed by Dhembare (2014) and Bachha et al. (2022).Organic matter plays a very important role in potassium availability though decomposition and mineralization processes.This was observed in case of T 7 which had the highest available potassium content.Termite mound soils have high CEC which also explains relatively high availability of potassium in the potting media with mound soil.The present findings corroborate with that of Dhembare (2014) and Bachha et al. ( 2022) who found 10.08 and 0.37 per cent increase in potassium availability of mound soil as compared to surrounding soil, respectively.The potting mixture after harvesting of the crop showed a decline in organic carbon, available nitrogen, phosphorus and potassium (Figure 2).Lower available nitrogen might be due to plant uptake, leaching and volatilization.Similarly low available phosphorus could be attributed to plant uptake.Again, luxurious consumption of potassium by the test crop might seem to be the valid reason for low amount of available potassium.Organic carbon gradually decreased most likely due to organic matter mineralisation.This might also be the reason for decline in pH.The EC also had a gradual decrease due to depletion of micronutrients as a result of plant uptake.Termite mound soil harbours soil microorganisms which play a vital role in organic matter decomposition and nutrient cycling which contributes greatly to microbial biomass carbon.High SMBC recorded in case of T 4 and T 7 was most likely due to the presence of beneficial microorganisms in termite mound soil.FYM also harbours a wide array of microorganisms indicating increased microbial biomass carbon content in case of T 7 .Issoufou et al. (2019) has demonstrated that termites significantly increase microbial biomass besides promoting significant activity of microbial decomposers leading to increase in degradation of soil organic matter.Dehydrogenases play a crucial role in the microbial respiration process and breakdown of organic matter.Due to presence of termite mound soil and relatively high amount of FYM, high dehydrogenase activity was observed in T 7 and T 4 .More or less similar findings were reported by Subi and Sheela (2020).Lowest dehydrogenase activity was observed in T 5 , most probably because of absence of FYM.PME activity in potting media ranged from 29.17 to 50.54 μg p-nitrophenol/g soil/h.This group of enzymes play a vital role in the mineralization process and cycling of phosphorus in soil ecosystems.High PME activity was observed in T 4 and T 7 because of the high proportion of termite mound soil and FYM.Increase in enzyme activity due to increase in organic matter content was reported by Lee and Wood (1971).The present findings were in line with those of Roose-Amsaleg et al. ( 2005) who observed high phosphatase activity in fresh mound as compared to mature mound.FDA activity is commonly used as an indicator of total microbial activity in soil.High FDA was noticed in T 4 preferably because of high microbial activity in termite mound soil.It was followed by T 7 most likely due to the presence of relatively higher proportion of FYM.Lowest FDA activity was observed in T 5 which could be attributed to the lack of organic matter.Urease is an enzyme that catalyses the hydrolysis of urea into ammonia and carbon dioxide.High urease activity was observed in T 7 which might be attributed to the high organic matter and microbial present in termite mound soil.Low organic matter content can make enzyme prone to biological degradation as reported earlier by Baligar et al. (1991).Lowest urease activity was recorded in T 5 probably due to the absence of organic matter.After harvesting of the crop at the end of crop season, biological activities seemed to increase (Figure 3).This might be attributed to the release of rhizodeposits and increase in microbial activity with time due to frequent irrigation and exposure to sunlight (Haldar and Sengupta, 2015).Out of all the potting media treatments, T 7 (Termite soil, sand and FYM (v:v:v / 1:1:2)) emerged out as the best one in terms of soil pH, organic carbon, available nitrogen, available phosphorus, available potassium, dehydrogenase enzyme activity, PME activity and urease activity while T 4 (Termite soil, garden soil and FYM (v:v:v / 2:1:1)) showed superiority with regards to PME activity, FDA activity and SMBC.
In case of vegetative growth parameters of the test crop, double pinching might have caused the suppression of apical growth and resulted in similar heights in all the three treatments.The results were in close agreement with Pant et al. (2022) who found double pinching to be the factor for minimum plant height in African Marigold.Highest plant spread was observed in T 7 while the lowest was observed in control (Table 3).High proportion of termite soil and presence of FYM might be the reason for the higher plant spread in case of T 7 .High availability of NPK, water holding capacity, organic matter content, good drainage and water retention were prevalent in T 7 .Highest numbers of branches (Table 3) were observed in T 7 which was statistically at par with T 4 .The lowest number was observed in control.Prevalence of adequate nutrient levels in potting media due to combined effect of termite soil and FYM and double pinching performed might have led to activation of lateral buds.Meena et al. (2015) and Khobragade et al. (2012) also reported that there was development of more side branches after pinching.T 7 and T 4 were statistically at par and had high number of leaves as compared to control.The number of leaves corresponded with the number of branches as more branching ensures a greater number of leaves.Supportive evidence regarding the number of leaves was reported by Singh et al. (2017) and Singh et al. (2015).Highest leaf area index was observed in T 7 while the lowest was observed in control.High leaf area index in T 7 might be because of good branching habit, more number of leaves, high specific leaf area and plant density.The findings of Yadav et al. ( 2018) was found to be similar with the current study and found the leaf area index to be 1.14 in African marigold.T 7 and T 4 recorded high stem diameters while control had the lowest (Table 3).Increased nutrient availability can increase plant growth including stem diameter which was easily and adequately available in both T 7 and T 4 treatments.A comparatively low nutrient content, water holding capacity and infiltration rate in addition to alkaline nature of potting media in control treatment may have contributed to significantly low stem diameter.Present findings were in conformity with Pant et al. (2022).The highest number of roots (statistically at par with each other) were observed inT 7 and T 4 while control recorded the lowest number of roots (Table 3).A corresponding trend was observed in case of root length and number of roots, i.e., T 7 and T 4 were statistically at par while control recorded the lowest root length.Presence of high proportion of termite soil and FYM in the selected potting media accounts for high nutrient availability, crumbly and porous soil structure to promote aeration and water drainage and proliferation of beneficial soil microorganisms.The treatments, T 7 and T 4 possess elevated microbial activity which might have enhanced root growth.Termites make the soil porous accounting for better root growth (Aparna et al., 2021).The first flower bud was observed in T 7 and T 4 potting media (statistically at par) and in control after 43.13 days (Table 3).The probable cause of early bud initiation may be due to rise in temperatures during the initiation of the reproductive phase.Light exposure in the field condition with ample sunshine hours and moisture with adequate nutrients from mixture of termite soil and FYM might have contributed to early initiation of flower bud.Sheoran et al. (2022) observed a similar result at 40.66 days.The delay in days to flower opening might have been influenced by double pinching.The delay in days to flower opening might have been influenced by double pinching.It can be assumed that pinching broke the apical dominance of the crop which led to utilization of energy for lateral branching and prevention of flower primordial development, hence, delaying the days to flower opening.While T 7 and T 4 showed early flowering opening, control required a longer duration (Table 3).This could be due to the difference in nutrient content of the treatments.The observations of Sheoran et al. (2022) were in consonance with the present findings, who reported opening of flower at 57.87 days with pinching performed at 2 weeks after transplanting.It was observed that there was a delay in the number of days taken from planting till full bloom (Joshna and Pal, 2015).This might be attributed to the performance of double pinching which delayed the reproductive growth.However, the number of days taken by T 4 and T 7 was comparatively less than control, whose reproductive growth was delayed the most.The variation in the treatments regarding the number of days might be because of the difference in the nutrient content of the potting media.Similarly, the flowering duration inT 7 and T 4 displayed the same mean values followed by control (Table 3).The results were akin to those reported by Srinivas and Rajasekharam (2020).The number of days from opening to fading of individual flower was observed to be 49.63 days in both the treatments.Environmental factors like rainfall and hormonal regulation might have affected the early fading of flower.The results are in accordance with Yadav et al. (2018).Similarly, flower bud diameter was high in both T 7 and T 4 .But the largest diameter and fresh weight of flower was recorded in T 7 .This might be due to superiority of T 7 in terms of nutrient content.Minimum bud diameter registered in control showed significant effect of the absence of termite mound soil on the bud diameter.The diameter of flowers and fresh weight might have been affected by the pinching as it diversifies energy to the lateral branches.However significant differences among the treatments might be due to the difference in the nutrient availability and chemicophysical properties of potting media.The control treatment produced the highest number of flowers followed by T 7 with T 4 having the least number of flowers.More number of flowers correlated with smaller flower size and weight.Similar works were carried out by Poudel et al. (2017) and Sheoran et al. (2022).Increase in the number of flowers might have been the reason for lesser fresh weight and diameter as there was lesser quantity of supply of food reserve to each of the individual flowers.As regards to yield, T 7 expressed the highest yield followed by T 4 and control (Table 3).It was mostly influenced by the number of flowers, fresh weight and flower diameter.
The highest B:C ratio as a potted plant and as a potting media was observed in T 7 to be 1.10 and 2.52, respectively.This is because of the highest net return in T 7 due to its best performance in growth parameters.From the observations of Table 3, it can be inferred that the potting media: T 7 was found to be significantly superior in terms of plant spread, leaf area index, fresh weight of flower, flower diameter and yield of flower but observed to be statistically at par with T 4 in terms of plant height, number of branches, number of leaves, stem diameter, number of roots, root length, root diameter, days to visibility of first flower bud, days to opening of flower, days to full bloom, flowering duration on plant, days from opening to fading of individual flower, number of flowers per plant and flower bud diameter.However, the control was found to be significantly superior in terms of number of flowers per plant.Economically, T 7 was also found to be another viable choice as compared to the other treatments.Hence, we recommend T 7 potting media to be used for commercial purposes for growing floriculture crops.

Conclusion
From the current research findings, one is assured of the multiple benefits that can be achieved by using termite mound soil as a potting media.Incorporating termite mound soil into agricultural systems represents a sustainable and eco-friendly approach to farming, particularly in regions with limited access to external inputs and low soil fertility.By harnessing the benefits of termite mound soil, farmers can improve soil fertility, enhance crop productivity, and reduce excessive dependence on synthetic pesticides.The rich nutrient

FIGURE 1
FIGURE 1 FIGURE 3 (A-D) Selected mounds of O. obesus for collection of termite mound soil.
while Keen Raczkowski box

TABLE 1
Physico-chemical properties, available nutrient content, and biological properties of termite mound soil based potting media.

TABLE 2
Simple correlation among physico-chemical and biological properties of potting media.

TABLE 3
Effect of potting media on vegetative and reproductive growth parameters of T. erecta cv.Inca Orange.

TABLE 4
Comparison of potting media properties before and after growing crop.

TABLE 5
Economics of potted plants and potting media using termite mound soil.
10.3389/fmicb.2024.1387434Frontiers in Microbiology 11 frontiersin.orgcontent of termite mound soil based potting media is a true example of conversion of "biowaste to biowealth." Being a low-cost technology, the invention can easily be explored by the farmers engaged in floriculture and ornamental nursery, peri-urban agriculture and rooftop gardening.