miR-144-3p Promotes Adipogenesis Through Releasing C/EBPα From Klf3 and CtBP2

MicroRNAs (miRNAs), a class of small non-coding RNAs, have been proved as novel and potent regulators of adipogenesis. A previous study has found out that miR-144-3p was a biomarker of type 2 diabetes, but the role of miR-144-3p in regulating adipogenesis was still unclear. In the present study, the expression of miR-144-3p increased in obese mice and during the 3T3-L1 differentiation process. Overexpression of miR-144-3p suppressed the expression of cell cycle regulatory factors and inhibited pre-adipocytes proliferation. Besides, overexpression of miR-144-3p accelerated lipid accumulation in adipocytes and positively regulated adipogenesis, which was also accompanied by increasing the expression of genes related to fatty acid synthesis and decreasing the expression of genes involved in fatty acid oxidation. Furthermore, luciferase activity assays indicated that miR-144-3p directly targeted Klf3 and CtBP2. The process was also confirmed by the mRNA and protein expression of Klf3 and CtBP2, which were suppressed by miR-144-3p. Furthermore, miR-144-3p targeting Klf3/CtBP2 would induce C/EBPα activity by releasing corepressors (Klf3 and CtBP2) from its promoter region. Moreover, we also observed that miR-144-3p could promote adipogenesis in mice injected with miR-144-3p agomir through tail-vein injection. Taken together, these results support that miR-144-3p can facilitate adipogenesis both in vitro and in vivo, which implies that miR-144-3p could be a target for therapeutic intervention in obesity and metabolic syndrome in the future.


INTRODUCTION
Epidemic obesity is a threat to human health in both developed and developing countries (Zimmet et al., 2001), and it is also associated with increased risks of type II diabetes, hypertension, and cardiovascular disease (Kopelman, 2000;Goran et al., 2003). Adipocytes are a major cellular component in fat tissue. It is well known that during the development of obesity, there is an inflammatory reaction of the adipose tissue together with an impairment of adipose cells differentiation (McLaughlin et al., 2007;Arner et al., 2011;Cordido et al., 2014;Hammarstedt et al., 2018). Moreover, adipocytes are emerging as one of the major targets in the prevention and treatment of obesity and related metabolic syndrome (Grundy, 2004). To this end, it is important to understand the molecular genetic mechanisms that regulate adipogenesis. During the last decade, researchers have found out that adipogenesis was governed by a tightly controlled cascade of transcription factors, which coordinated hundreds of downstream genes (Farmer, 2006). In general, CCAAT/enhancer binding protein (C/EBP) β and C/EBPδ are first activated during the early stage of adipogenesis, which will directly activate C/EBPα and peroxisome proliferatoractivated receptor γ (PPARγ) expression. Then, the latter factors, C/EBPα and PPARγ, will induce each other's expression by a positive feedback loop, which results in activating downstream target genes related to promoting and maintaining adipogenesis [such as adipocyte fatty acid-binding protein 2 (aP2)] (Rosen et al., 2000;Rosen and MacDougald, 2006). microRNAs (miRNAs), a class of non-coding endogenous RNA molecules, post-transcriptionally regulate gene expression by binding to 3 UTR of specific mRNA targets, which results in either a translational repression or a cleavage of the affected mRNA (Bartel, 2004). Interestingly, some miRNAs have been verified to affect adipocyte biology, adipocyte differentiation, and adipose tissue function. For instance, miR-143, miR-17-92 cluster, miR-30c, and miR-24 promote adipocyte differentiation by negatively regulating extracellular signal-regulated kinase 5 (ERK5) (Esau et al., 2004), tumor-suppressor Rb2/p130 (Wang et al., 2008), activin receptor-like kinase 2 (ALK2) (Karbiener et al., 2011), and mitogen-activated protein kinase 7 (MAPK7) (Jin et al., 2016), respectively. miR-27b, miR-200b, miR-26b, and miR-215 impair adipocyte differentiation by targeting PPARγ (Karbiener et al., 2009), Kruppel like factor 4 (Klf4) , phosphatase, tensin homolog gene (PTEN) , and fibronectin type III domain containing 3B (FNDC3B) (Peng et al., 2016), respectively.
A previous study has found out that miR-144-3p was highly up-regulated in type 2 diabetes (T2D) and could impair insulin signaling (Karolina et al., 2011). The phenotype of insulin resistance was closely related to adipocyte differentiation and obesity (Kahn and Flier, 2000;Fu et al., 2005). Besides, the expression of miR-144-3p was positively correlated with adipocyte volume in both lean and obese pigs according to our previous study (Li et al., 2012). However, the epigenetic mechanism underlying the function of miRNA-144-3p in governing adipogenesis is not well clarified at present. Thus, in vivo and in vitro experiments were operated to explore the role of miRNA-144-3p in adipogenesis in this study. Our results indicate that miR-144-3p is an important positive regulator of adipogenesis. Luciferase reporter assays demonstrate Kruppel like factor 3 (Klf3) and carboxy-terminal binding protein 2 (CtBP2), the corepressors of C/EBPα (Sue et al., 2008;Wang et al., 2015), are direct target genes of miR-144-3p. Therefore, these results suggest that miR-144-3p may be a potential target for therapeutic intervention in obesity and metabolic syndrome.

Experiment Animals
All animal experimental procedures and sample collections were performed in accordance with the guidelines of Institutional Animal Care and Use Committee of College of Animal Science and Technology of Sichuan Agricultural University, Sichuan, China, under permit NO. DKY-B20131403 (Ministry of Science and Technology, China, revised in June 2004). In the obesity model study, two groups of 7-week-old male Kunming mice (n = 8) were fed with a high-fat diet (HFD) or received normal chow (NCW), respectively, for 3 months. In the in vivo assay, two groups of male Kunming mice (n = 3) were tail-vein injected with miR-144-3p agomir or agomir control (RiboBio, Guangzhou, China), respectively. Injections were given every 3 days and lasted for 3 weeks, with a dose of 80 mg/kg body weight. During the experiment, mice were given free access to food and water under controlled light and temperature conditions. Mice were sacrificed by cervical dislocation, and adipose samples were collected for RNA extraction and histological analysis.

Cell Culture and Transfection
3T3-L1 cells were maintained, differentiated, and transfected as described in our previous study . Briefly, 3T3-L1 cells were maintained in DMEM containing 100 U/ml penicillin, 100 µg/ml streptomycin, and 10% fetal bovine serum at 5% CO 2 humidified atmosphere (37 • C). For differentiation, cells were cultured in DMEM supplemented with 10% fetal bovine serum and MDI (1 µM dexamethasone, 0.5 mM 3isobutyl-1-methylxanthine, 1 µM dexamethasone, and 5 µg/ml insulin) when cells reached confluence. After 2 days, the culture medium was replaced with DMEM containing 10% FBS and 5 µg/ml insulin every 48 h until the pre-adipocytes totally differentiated into mature adipocytes (day 8). For transfection, short double-stranded RNAs (miRNA mimics) and their OMe-modified antisense oligonucleotides (miRNA inhibitors) of miR-144-3p were synthesized by Ribobio (Guangzhou, China). The first transfection was operated when 3T3-L1 reached confluence (begin to differentiate). The transfection was carried out using lipid carrier lipofectamine 2000 (Invitrogen, Carlsbad, CA, United States) following the manufacturer's instructions. Briefly, mimics or inhibitors (20 nM) were mixed with lipid carrier (2:1, v/v), then incubated in Opti-MEM (Invitrogen, Carlsbad, CA, United States) medium for 20 min. Then, 3T3-L1 cells were incubated with the oligonucleotides and lipofectamine complex for 5 h transfection course, and then replaced the mixtures with normal differentiation medium. The transfection was carried out every second day when the medium was replaced.

Oil Red O Staining and Triglyceride Assay
Briefly, differentiated 3T3-L1 cells were gently washed twice with cold 1 × PBS, fixed in 4% paraformaldehyde for 30 min, and washed twice with deionized water. Then, cells were stained with 60% saturated Oil Red O for 1 h, washed twice with deionized water, and photographed using an Olympus IX53 microscope (Olympus, Tokyo, Japan). For the triglyceride assay, cells were washed with cold 1 × PBS, detached from the plates using a cell scraper, and homogenized by sonication (1 min). Total triglyceride quantification was performed with Infinity Triglycerides Reagent (NO. TR22421, ThermoFisher Scientific, San Jose, CA, United States) according to the manufacturer's protocol.

CCK-8 and EdU Assays
The proliferation rates of 3T3-L1 were measured using Cell Counting Kit-8 (CCK-8; Dojindo, Tokyo, Japan) according to the manufacturer's instructions. Briefly, transfected cells (miR-144-3p mimic and inhibitor) were seeded in 96-well plates with a density of 2000 cells/well, then incubated in the growth medium containing 10% CCK-8. Cell samples were incubated at 37 • C and collected at 6, 12, 24, 48, and 96 h. The absorbance of the sample was measured at 450 nm using a microplate reader (Bio-Tek Instruments, Inc., Winooski, VT, United States). For Edu assay, transfected cells (24 h post-transfection) were incubated in the growth medium containing 50 mM Edu reagent (RiboBio, Guangzhou, China). After 24 h Edu staining, samples images were captured using a Nikon TE2000 microscope (Nikon, Tokyo, Japan).

Quantitative RT-PCR Analysis
Quantitative RT-PCR (q-PCR) was used to measure mRNA and microRNA (miRNA) expression. Total RNA was extracted from adipose tissue and cell samples using Trizol Reagent (Invitrogen, Carlsbad, CA, United States), and was further purified with RNeasy columns (Qiagen, CA, United States) according to the manufacturer's protocol. Subsequently, mRNA and miRNA were reverse-transcribed to cDNA using the PrimeScript TM RT Master Mix Kit and the PrimeScript miRNA RT-PCR Kit, respectively (TaKaRa, Dalian, China). Quantitative PCR was performed using the SYBR Green Real-time PCR Master Mix (TaKaRa, Dalian, China) by CF96 Real-Time PCR Detection System (Bio-Rad, Hercules, California, United States). β-actin and U6 were served as endogenous controls for mRNA and miRNA expression, respectively. The 2 − Ct method was used to calculate the relative expression levels of mRNA and miRNA (Livak and Schmittgen, 2001). All PCR primer sequences were shown in Table 1.

Cell Cycle Assay
Briefly, cell samples were fixed in chilled ethanol overnight after resuspending in PBS with 1% normal goat serum (NGS). After two rounds of washing, cells were resuspended in PBS with 1% NGS with 120 µg ml −1 propidium iodide and 10 µg ml −1 RNase A, and then incubated for 30 min at 37 • C. Subsequently, the cell cycle was measured by flow cytometry (FACScan, BD Biosciences).

Western Blotting
Briefly, cells were lysed on ice using a commercial lysis buffer (Sigma, Louis, Mo, United States), according to the manufacturer's instructions. Collected proteins were boiled in 5× SDS buffer for 5 min, then separated by 10% SDS-polyacrylamide gel, and transferred to a PVDF membrane (Bio-Rad Co., United States). Subsequently, transferred membranes were blocked by TBST containing 5% non-fat dried milk for 2 h at 37 • C, and then immunoblot (incubated overnight at 4 • C) was conducted with primary antibody (Abcam, Shanghai, China). Then, immunoblot membranes were washed three times with TBST for 15 min and then incubated with horseradish peroxidase-conjugated secondary antibody for 1 h at 37 • C. The blots were visualized by DAB reagent (Boster, Wuhan, China) according to the manufacturer's instructions.

Statistical Analysis
Data were analyzed by SPSS software (21.0 version). All data were presented as means ± standard deviation (SD). Differences in groups were analyzed with Student's t-test. Differences were considered statistically significant at p < 0.05.

RESULTS AND DISCUSSION
miR-144-3p Inhibits 3T3-L1 Pre-adipocyte Proliferation It is well known that the biological process of adipocyte proliferation and differentiation is the foundation for the accumulation of lipids in adipose tissue (Rosen and MacDougald, 2006). However, miRNAs that related to regulating pre-adipocyte proliferation and differentiation were proved to have opposite effects. For example, miR-125b-5p and miR-26b could inhibit pre-adipocytes proliferation but promote the differentiation Ouyang et al., 2015). miR-199 and miR-125a-5p could promote pre-adipocytes proliferation but inhibit the differentiation Xu et al., 2018). In this study, to explore the potential role of miR-144-3p in the proliferation of pre-adipocytes, firstly 3T3-L1 pre-adipocytes were used to transiently transfect miR-144-3p mimic or inhibitor, respectively. 3T3-L1 pre-adipocyte cell line is a widely used adipocyte model, which is an ideal approach to understand the molecular mechanism governing adipogenesis (Farmer, 2006). The transfection efficiency in 3T3-L1 was shown in Figure 1A, miR-144-3p mimics or inhibitors significantly increased (approximate seven times) or suppressed (approximate nine times) the expression levels of miR-144-3p when compared to the negative control group, respectively. These data suggested that the transfection experiment operated in this study was a great success and ensured the data reliability in subsequent experiments. Next, Counting Kit 8 (CCK-8) and 5-ethynyl-20-deoxyuridine (EdU) staining were also used to evaluate the function of miR-144-3p on pre-adipocyte proliferation. As shown in Figure 1B, after 24 h transfection, the growth rate of 3T3-L1 pre-adipocytes was significantly decreased or increased in mimics or inhibitor group, respectively, when compared to the control group. This finding was also confirmed by EdU analysis. As shown in Figures 1C,D, overexpression of miR-144-3p could significantly suppress the number of EdU-positive cells when compared to the control group. However, knockdown of miR-144-3p significantly increased the ratio of EdU-positive cells. Besides, to confirm the function of miR-144-3p on pre-adipocyte proliferation, the expression levels of some important cell cycle regulatory factors were also detected. For example, cyclindependent kinases (such as CDK4), Cyclin D1 and Cyclin E have been recognized as key regulators of cell growth and proliferation in eukaryotes, which are required for G1/S and G2/M transitions in mammalian cells (Resnitzky et al., 1994;Suzuki et al., 2000). As shown in Figure 1F, the results are consistent with the observations above, and qRT-PCR analysis indicated that knockdown of miR-144-3p could remarkably increase the Cyclin D1, Cyclin E, and CDK4 expression. While overexpression miR-144-3p significantly suppressed the expression of these cell cycle regulatory factors. Furthermore, the cell cycle distribution was investigated with miR-144-3p overexpression or knockdown, respectively. Flow cytometry analysis showed that overexpression of miR-144-3p could increase the ratio of cells in the G0/G1 phase and decrease the ratio of cells in the G2/M phases, and vice versa in the miR-144-3p knockdown group ( Figure 1E). Therefore, these results collectively suggest that miR-144-3p may inhibit 3T3-L1 pre-adipocyte proliferation.

miR-144-3p Promotes 3T3-L1 Pre-adipocyte Differentiation
To further investigate the effect of miR-144-3p on adipogenesis, obese mice were induced by a high-fat diet (HFD). As shown in Figure 2A, the body weight significantly increased in HFD feeding group when compared to the normal chow (NCW) receiving group (p < 0.05), which indicated that mice could become obese under HFD. As expected, the expression levels of two key adipogenic genes, PPARγ and C/EBPα (Farmer, 2006), and an adipocyte-specific gene, aP2 (Hotamisligil et al., 1996), were significantly elevated in the HFD group when compared to the NCW group. Interestingly, the expression level of miR-144-3p significantly increased in the adipose tissue from HFD-fed mice ( Figure 2B). This finding also agreed with our previous study, which reported that miR-144-3p expression had a positive correlation with adipocyte volume in both lean and obese pigs (Li et al., 2012). Subsequently, to test whether the expression pattern of miR-144-3p in vivo could also be observed in vitro, the expression of miR-144-3p was investigated during 3T3-L1 pre-adipocyte differentiation. As shown in Figure 2C, the expression level of miR-144-3p markedly increased during adipogenic differentiation. As expected, overexpression of miR-144-3p could significantly promote lipid accumulation in 3T3-L1 and accelerate the process of adipogenesis according to the Oil Red O staining analysis ( Figure 2D). In accordance with these findings, the triglyceride content in 3T3-L1 cells was also significantly increased in the miR-144-3p mimic group (p < 0.05), and significantly decreased in the inhibitor group (p < 0.01) (Figure 2E). To further confirm the function of miR-144-3p on adipogenesis, expression levels of some adipogenesis related regulators and markers were detected. As shown in Figure 2F, the expression of aP2, C/EBPα, and PPARγ had higher levels in the miR-144-3p mimic group when compared to the control group, and an opposite result was observed in inhibitor group. Moreover, fatty acids served as an important component in adipose tissue, and both increasing fatty acid synthesis and decreasing fatty acid oxidation can enhance lipid accumulation in adipocytes (Hems et al., 1975;Abu-Elheiga et al., 2001). Here, as expected, the expression of genes involved in fatty acid synthesis  was significantly increased in the mimic group, but the expression of genes associated with fatty acid oxidation  was suppressed in the mimic group ( Figure 2G). Taken together, these results indicate that miR-144-3p can promote the differentiation of 3T3-L1 preadipocytes by diminishing fatty acid production and enhancing fatty acid oxidation.

miR-144-3p Promotes 3T3-L1 Adipogenesis by Targeting Klf3 and CtBP2
Our studies above have proved that miR-144-3p could inhibit pre-adipocytes proliferation and promote its differentiation, but the target genes regulated by miR-144-3p were still unclear (Ling et al., 2011). Therefore, to further explore the potential epigenetic mechanism underlying miR-144-3p promoting 3T3-L1 adipogenesis, computational prediction programs, like Pic Tar, targetScan, and miRanda, were used to predicate potential target genes of miR-144-3p. All the target genes must be perfectly matched to the seed sequence (2-8 site) of miR-144-3p by each software. As shown in Figures 3A,B, among the predicted target genes, both Klf3 and CtBP2 contained a complementary seed sequence of miR-144-3p in the 3 -UTR region. Interestingly, a previous study reported that Klf3 could directly bind to the endogenous C/EBPα promoter and repress its activity, and overexpression of Klf3 could suppress adipogenesis in 3T3-L1 cells (Sue et al., 2008). Zhu et al. (2018) also proved that miR-20a could promote adipogenic differentiation by targeting Klf3 in bone marrow stromal cells.
CtBP family proteins are known as transcriptional corepressors (Chinnadurai, 2002;Vernochet et al., 2009). Previous studies have found out that CtBP2 could directly bind to the adipogenic , and C/EBPα in 3T3-L1 cells treated with miR-144-3p mimic or inhibitor. All data were expressed as means ± SD (error bars represent the SD from three independent experiments). * p < 0.05, * * p < 0.01. maker (C/EBPα) and down-regulate its expression, which results in suppressing adipocyte differentiation (Wang et al., 2015). Interestingly, a previous study reported that Klf3 repressed transcription accompanying by recruiting CtBP corepressors in adipogenesis (Sue et al., 2008). In this study, homology analysis was used, and it proved that the two complementary sites between Klf3/CtBP2 and miR-144-3p were highly conserved among different species (Figures 3A,B). This result suggests that the epigenetic regulation is possibly a universal model in mammals. Furthermore, a double fluorescent reporter assay was performed to confirm that miR-144-3p suppressed Klf3 and CtBP2 activity by binding to the 3 -UTR. As shown in Figures 3C,D, the relative luciferase activity was significantly decreased in HeLa cells co-transfected with WT-Klf3/WT-CtBP2 and miR-144-3p mimic (p < 0.05), and the reducing of luciferase activity had a dose-dependent manner of mimic One of the pathway of miR-144-3p to promote adipocyte differentiation. All data were expressed as means ± SD (error bars represent the SD from three independent experiments). * p < 0.05, * * p < 0.01.
concentration. However, the luciferase activity didn't decrease in HeLa cells co-transfected with MUT luciferase plasmids (Figures 3C,D). Furthermore, overexpression or knockdown of miR-144-3p in 3T3-L1 cells could significantly suppress or promote Klf3/CtBP2 expression in mRNA level and protein level, respectively (p < 0.01) (Figures 3E,F). Besides, considering both Klf3 and CtBP2 are corepressors of C/EBPα, C/EBPα expression was detected in 3T3-L1 transfected with miR-144-3p mimic and inhibitor. In agreement with the conjecture, the protein expression level of C/EBPα, a crucial and pivotal regulator of adipogenesis (MacDougald et al., 1995), was significantly promoted by miR-144-3p ( Figure 3F). Taken together, these data suggest that miR-144-3p promotes adipocyte differentiation by directly targeting the 3 -UTR of Klf3 and CtBP2, which results in releasing C/EBPα from Klf3 and CtBP2.

miR-144-3p Promotes Adipogenesis in vivo
To test whether the effect of miR-144-3p on adipogenesis in cell culture could also be observed in vivo, miR-144-3p expression in vivo was up-regulated by injecting miR-144-3p agomir in mice through the tail vein. Three weeks after the first injection (corresponding to 3 days after the last injection), the mouse body weight had no significant difference between the miR-144-3p agomir and negative control group. But the whole body fat mass in the miR-144-3p agomir group was significantly higher than the negative control (Figures 4A,B). To confirm that miR-144-3p was successfully overexpressed in adipose tissue by tail injection of agomirs, the expression levels of miR-144-3p in multiple adipose tissues were studied. As shown in Figure 4C, the expression level of miR-144-3p significantly heightened in inguen, gonad, and perirenal fat when mice were tail-injected with miR-144-3p agomirs (p < 0.01). Based on this finding, the adipocyte volume of gonads fat tissue between two different groups was also measured. As shown in Figures 4D,E, mice injected with miR-144-3p agomir have a higher adipocyte volume than that in the negative control mice. Besides, miR-144-3p agomir could significantly increase serum levels of total TC, TG, and LDL when compared to the negative control (Figures 4F-H).
These blood indicators were demonstrated to be related to the phenotype of obesity in previous studies (Tan et al., 2017;Du et al., 2018). Therefore, these studies suggest that miR-144-3p also have the function of promoting adipogenesis in vivo.
To further confirm this finding, the expression levels of genes related to adipogenesis of the two groups were also measured. As shown in Figure 4I, the expression levels of three key adipogenic genes (PPARγ, C/EBPα, and aP2) were elevated in the agomir group when compared to the negative control group. For example, PPARγ is widely known as a necessary factor for both adipogenesis and HFD induced obesity (Kubota et al., 1999;Jones et al., 2005). Besides, PPARγ is involved in glucose metabolism via an improvement of insulin sensitivity (Kubota et al., 1999;Hevener et al., 2003). Therefore, synthetic PPARγ agonists (thiazolidinediones, glitazones) are clinically used as insulin sensitizers to treat patients with type 2 diabetes (Kahn et al., 2006). Furthermore, miR-144-3p also significantly promoted the expression of genes associated with fatty acid synthesis and repressed the expression of genes involved in the fatty acid oxidation, when compared to the control group ( Figure 4J). As expected, the research confirmed that the effect of miR-144-3p on adipogenesis in 3T3-L1 could also be observed in vivo. Taken together, these data elucidate a possible pathway used by miR-144-3p to regulate adipocyte differentiation, and the plausible regulatory network is shown in Figure 4K.

CONCLUSION
In summary, our results confirmed that miR-144-3p inhibited 3T3-L1 pre-adipocyte proliferation, and promoted differentiation by directly targeting Klf3 and CtBP2. miR-144-3p would promote C/EBPα expression by releasing Klf3 and CtBP2 from C/EBPα promoter region. Furthermore, miR-144-3p was shown to upregulate genes associated with fatty acid synthesis, and to downregulate genes involved in fatty acid oxidation. In addition, the effect of miR-144-3p on adipogenesis can also be observed in vivo by injecting with miR-144-3p agomir through the tail vein. Thus, deeper studies regarding the in vivo function of miR-144-3p should be performed to evaluate this molecule as a potential target for obesity.