Immunomodulatory activities of Carica papaya leaf juice in AG129 mice infected with a clinical dengue virus isolate
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1
Institute for Medical Research, Ministry of Health (Malasiya), Malaysia
Background
Carica papaya leaf has been associated with immunomodulatory activities by several studies but limited to chemically induced inflammatory in vitro and in vivo models. Therefore, this study was initiated to investigate the immunomodulatory effect of the C. papaya leaf juice in a mouse model of dengue virus (DENV)-infection.
Methods
A mouse model of DENV-infection was established by intraperitoneal inoculation of clinical DENV-2 isolate in AG129 mice, lacking type I and II interferon receptors. The infected mice were orally treated with 500 mg/kg/day and 1000 mg/kg/day of freeze-dried C. papaya leaf juice for three days starting on day 1 post-infection. The platelets and leukocyte components were counted by microscopy technique. Plasma cytokines and chemokines levels were determined by multiplex immunoassay technique. The level of viral RNA, cytokines and chemokines in the liver, kidney, spleen and brain tissues were determined by quantitative reverse transcription PCR.
Results
The inoculation of DENV-2 clinical isolate caused a symptomatic but non-lethal illness to AG129 mice accompanied by the production of proinflammatory cytokines and chemokines in the plasma and organs tissues. Treatments with 500 mg/kg and 1000 mg/kg of freeze-dried C. papaya leaf juice increased the platelet and leukocyte counts in DENV-2 infected AG129 mice. A significant decrease (p<0.05) in viral RNA level was detected in the liver and kidney of infected AG129 mice treated with 1000 mg/kg of freeze-dried C. papaya leaf juice. C. papaya treatment also significantly decreased (p<0.05) the levels of certain cytokines and chemokines in plasma, liver and kidney tissues of infected AG129 mice.
Conclusion
The findings suggest the beneficial effect of C. papaya leaf juice in dengue treatment could be due to its immunomodulatory properties. Hence, identification of compounds that are responsible for the immunomodulatory activities of C. papaya leaf is in need.
Keywords:
Carica papaya,
Antiviral activity,
dengue virus infection,
AG129 mice,
Immunomodulatory activity,
cytokine
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Infectious diseases
Citation:
Mohd Abd Razak
M,
Norahmad
N,
Md Jelas
N,
Mohmad Misnan
N,
Muhammad
A,
Jusoh
B,
Chau Dee
T,
Mat Ripen
A,
Thayan
R and
Syed Mohamed
A
(2019). Immunomodulatory activities of Carica papaya leaf juice in AG129 mice infected with a clinical dengue virus isolate.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00107
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Received:
13 Oct 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Mohd Ridzuan Mohd Abd Razak, Institute for Medical Research, Ministry of Health (Malasiya), Kuala Lumpur, Malaysia, eezoo79@gmail.com