Isolation and antimicrobial activity of probiotic strains for caries prevention
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1
Department of Basic Sciences & Oral Biology, Faculty of Dentistry, Universiti Sains Islam Malaysia, Malaysia
Background
Probiotics can be described as live microorganism which when consumed in adequate amounts, it will confer health benefits to the host. The benefits arising from probiotics has been mostly shown on gut health. However, its function to oral health is still poorly investigated. The aim of this study was to isolate lactic acid bacteria (LAB) from local fermented food and to determine its antibacterial activity, in order to select candidate probiotic strains for preventing caries.
Methods
The probiotic strains were isolated from five local fermented food which are tapai ubi, tapai pulut, rebung, thosai and taucu. Ten-fold serial dilutions of the fermented food samples were made in sterile peptone water before plating on de Man Rogosa and Sharpe (MRS) agar. The pure cultures were then randomly picked and biochemically identified. Then, the antibacterial activity of LAB against Streptococcus mutans were assessed by using disk diffusion method.
Results
A total of 120 LAB were isolated from five different fermented foods. The morphology of the isolates were circular, convex, dull opaque white or translucent white. Gram-staining identification showed that the isolates were Gram-positive rods. Of the 120 LAB isolates, 6 strains displayed moderate to strong antibacterial activity against S. mutans with the inhibition zones ranging from 7-12 mm.
Conclusion
The antibacterial activity demonstrated from LAB isolated from local fermented foods suggests that the isolates from local fermented foods possess antibacterial properties against pathogen responsible for causing dental caries. These strains are currently investigated in depth to assess whether they can be fully characterized as probiotics.
Keywords:
Probiotics,
Lactic acid bacteria,
Dental Caries,
Streptococcus mutans,
Oral Health
Conference:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”, Putrajaya, Malaysia, 3 Dec - 5 Feb, 2019.
Presentation Type:
Poster Presentation
Topic:
Miscellaneous
Citation:
Abdul Rahman
N,
Mohd Hanafiah
R,
Abdul Ghafar
S and
Abdul Rashid
N
(2019). Isolation and antimicrobial activity of probiotic strains for caries prevention.
Front. Pharmacol.
Conference Abstract:
International Conference on Drug Discovery and Translational Medicine 2018 (ICDDTM '18)
“Seizing Opportunities and Addressing Challenges of Precision Medicine”.
doi: 10.3389/conf.fphar.2018.63.00103
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Received:
19 Oct 2018;
Published Online:
17 Jan 2019.
*
Correspondence:
Dr. Nor Zaihana Abdul Rahman, Department of Basic Sciences & Oral Biology, Faculty of Dentistry, Universiti Sains Islam Malaysia, Nilai, Malaysia, zaihana@usim.edu.my