Can South Asian Diet Leads to Healthy Brain Ageing

1) Genetic Diagnostic and Research laboratory, Department of Anatomy, FacultyofMedlcal Sciences (FMS),UnlverjltyofSr1 Jayewardenepura (USJP), Sri Lanka; 2} Departmentof Neuropathology, National Institute of Mental Health & Neurosriences, Banga)ore,lndia;3) School of Medicaland MolecularBlosciences, University ofTechnology, Sydney, Australia; 4] Independent Researcher.Consultant, Biostatistics, Colombo, Sri Lanka; S) Departmentof Judicial Medical Office, Colombo South Teaching Hospital, Colombo; 6) Departmentof Pathology, FMS, US] P; 7) Departmentof Pharmacology College of Medicine, Seoul National University, Seoul and NRI, Gachon University, South Korea; 6) Departmentof Translational Neuroscience, Faculty Health, Medicine and LifeSdences,MaastrlchtUnlverslty, Maastricht, The Netherlands.


Introduction
Traditional South Asian diet is rich in pulses, cereals, fruits and vegetables.To the best of our knowledge this is the first brain autopsy study that has investigated the possible protective effect of South Asian diet on ageing cytoskeletal pathologies.

❖ Statistical Analysis
❖ The relationship between black tea consumption pattern (£2-3cups/day referred as "light tea drinkers" and >2-3cups/day referred as "heavy tea drinkers") and AD related lesions including square root values of NFTs count, SPs count and total CAA score was determined using two sample independent t-te s t > Binary logistic regression was used to find the relationship between cerebrovascular pathologies and consumption pattern (pure black tea, green-yellow vegetables and fish) of the deceased in addition to other risk factors such as genetic factors [apolipoproteln E ( A p o E ), angiotensin converting enzyme (A C E ), methylenetetrahydrofolate reductase (M T H F R ) and factor V Leiden ( F V L )), past medical history (diabetes, high blood pressure or hypertension, high cholesterol, ischemic heart disease and stroke), and health habits (smoking and alcohol consumption).Due to the small sample size, each factor was individually tested controlling for age and sex.
( ❖ Mean age at death between light and heavy tea drinkers was also tested with Mann Whitney U test \ as these pathologies are age-dependent and the p value was not significant (>0 05}.

Conclusion
Despite methodological limitations, our findings revealed a possible protective effect between diet Ceylon Tea, Green-yellow vegetables; and both Alzheimer and cerebrovascular pathologies in aging brains that warrants future studies to ascertain healthy eating habits among the South Asiah population.
Introduction: Traditional South Asian diet is rich in pulses, cereals, fruits and vegetables.
To the best of our knowledge this is the first brain autopsy study that has investigated the possible protective effect o f South Asian diet on ageing cytoskeletal pathologies.l Methods: Human brain samples from 76 subjects aged >50yrs were used to assess atherosclerosis of the circle o f Willis (CW), and out of that 50 brains aged >60yrs were subjected to neuropathological diagnosis using histopathological/ immunohistochemical techniques.Alzheimer's disease (AD) related neuropathological changes were counted in specific neuroanatomical regions.Antimortem questionnaire was administrated to obtain the consumption pattern of pure black tea, green-yellow vegetable, and fish, o f the deceased via kin.
Results: Frequent consumption o f pure black tea >4cups/day showed a significant reduction in P-amyloid (AP) accumulations-senile plaque counts in the region o f entorhinal cortex (p=0.009),superior frontal gyrus (p=0.041) and in all region (p=0.04)and the average cerebral amyloid angiopathy, grades in cortical and leptomeningeal region (p=0.037)compared to decedents who had consumed lightly (<2-3cups/day) with 2 sample independent t-test.Moderate consumption of green-yellow vegetables (l-6times/week) showed a significant negative association with moderate and severe atherosclerosis o f CW with the odds of 017 [p=0 011, 95% confident level (Cl) = 0 04-0-67] adjusted for age and sex.
Conclusions Despite methodological limitations, our findings revealed a possible protective effect between diet and both Alzheimer and cerebrovascular pathologies in aging brains that warrants future studies to ascertain healthy eating habits among the South Asian population. 2 rearMSIon controlled for age and 3ex.P value.<0.2 are only presented.I Atherosclerosis of the circle of Willis (CW) was graded in all samples based on degree of stenosis of the each component arteries and gross visual Inspection as none, mild, moderate & severe (WHO, 1958).

Pure Black Tea consumption Reduced Ap accumulations/ depositions Opens newavenue to Investigate the anti plaque effect/ neuroprotectlve effect ofCeylon blackteaonAO (II) Effects of moderate consumption of green-yellow vegetables on cerebral atherosclerosis
I Binary logistic regression controlled for age and sex.P value.<0.2 are only presented.I (