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Class Switch Recombination Process in Common Variable Immunodeficiency

Asghar Aghamohammadi1*, Hassan Abolhassani1, Payam Mohammadinejad1, Amir Salek2, Seyed Mohammad Moazzeni2 and Babak Mirminachi1
  • 1 Tehran University of Medical Science, Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children’s Medical Center, Iran
  • 2 Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Iran

Common variable immunodeficiency (CVID) is a heterogeneous group of disorders characterized by hypogammaglobulinemia and recurrent bacterial infections. Impaired antibody production in these patients is due to defect in differentiation of naïve B cell into plasma cells. Class switch recombination (CSR) which plays a critical role in the production of different Immunoglobulin classes may be defective in a group of CVID patients. The aim of this study was to investigate the CSR process of CVID patient’s B lymphocytes, by evaluating the expression of IgE mRNA and protein in an IgE inductive medium. Peripheral blood mononuclear cells (PBMCs) from twenty nine CVID patients and 21 healthy controls were isolated and cultured in the presence of rhIL-4 and CD40L. IgE mRNA and IgE protein were measured by RT-PCR and ELISA techniques, respectively. Normal production of IgE mRNA was recorded in 23 out of 29 patients (79.31%) as well as all controls; while the remaining 6 patients (20.69%) were unable to express IgE mRNA indicating a defect in CSR. PBMCs of 16 out of 29 patients (55.2%) could not produce normal values of IgE comparing with controls after stimulation by IL-4 and CD40L. IgE production is impaired in most of the CVID patients. Defects in post CSR (IgE mRNA transcription) processes such as protein production and secretion can be the causative mechanism of CVID in patients with normal mRNA and impaired protein productions. Determination of these defects can help to clarify the various underlying mechanisms in etiology of CVID.

Keywords: Common Variable Immunodeficiency, Class switch recombination, Immune dysregulation, IgE mRNA transcription, B-cell differentiation

Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013.

Presentation Type: Abstract

Topic: Adaptive Immunity

Citation: Aghamohammadi A, Abolhassani H, Mohammadinejad P, Salek A, Moazzeni S and Mirminachi B (2013). Class Switch Recombination Process in Common Variable Immunodeficiency. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00353

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Received: 16 Mar 2013; Published Online: 22 Aug 2013.

* Correspondence: Prof. Asghar Aghamohammadi, Tehran University of Medical Science, Research Center for Immunodeficiencies, Pediatrics Center of Excellence, Children’s Medical Center, Tehran, Iran, aghamohammadi@tums.ac.ir