Role of Organic Acids on Phosphorus Fractions in Silty Clay Loam Soil

Submission Track Abstract This study was conducted to evaluation the role of the organic acids in the phosphorus fractions in silty clay loam texture. The laboratoryexperiment was conducted by adding the organic acids (Humic acid ,Citric acid ,Oxalic acid , Malic acid ,Acetic acid and Lactic acid )to the fertilized soil with conc. super phosphate fertilizer with 100 kg h -1 level with concentrations (0,15,30,45 and60)mg L -1 ,except Humic acid (500,1000,1500 and 2000) mg L -


Introduction
Phosphorus is one of the necessarynutrient elementsof plant growth ,especial in aridand semi-arid soils ,amount of P in soil is depended on operations of weathering ,quality and quantity of clay minerals ,oxides, organic matter and calcium carbonate (1).Phosphorus can be found in the soil with multi forms as soluble , adsorption by clay minerals,CaCO 3 ,Fe and Al Oxides ,and sedimentor as calcium and Fe and Al phosphate ,combined with organic matter ,degree combined ofPwith colloids of organic and mineral soil were by physiochemical properties (2). The availability and forms of P in soil are changed according to operation fertilization and soil management .The phosphate fertilization led to increase the available phosphorus from( 3 -20)mg kg -1 at addition 26 kg Ph -1 (3).type and the level of addingphosphate fertilizer has an effect in its different forms and respond of production for its (5).Many of researchers (6)(7)(8)(9)were found on relation significant positive and negative between forms ofP and singular of growth to different crops depending on nature and environment of soils . The organic acids are affected in forms of P in the soils during the ranges of reactions such as: soluble of compound the sedimentation phosphate , *The research is past of Ph. D for 3 rd author . mineralization of organic phosphorus, decreasing of soil pH and attention ions of Ca ++ ,Fe ++ ,AL +++ (10) then increasing of phosphorus availability in soil .this research was conducted to evaluationrole of Organic acids in the availability of the phosphorus in the silty clay loam texture.

Materials and Methods.
Soil studied Soil texture was salty clay loam it was took from surface layer (0-30) cm from the fields in agriculture collage /Wassit university and air dried was sieved by 2mm, then conducted on its of general analysis which show in the table below (1). Role of organic acidsof Phosphorus fractions in soil. 75 gm of soil was air dried and put in plastic pots with 100cm 3 capacity .Five organic acids with low molecular weightwere added they were included Citric acid ,Oxalic acid ,Malic acid ,Acetic acid and Lactic acid with levels( 0,15 ,30,45,and 60) mg L -1 . Humic acid with high molecular weightwas used with levels( 0,500,1000,1500 and 2000) mg L -1 for soil of treatment with 100 kg h -1 . Moisture of pot was completed to field capacity limit .samples were incubated on 30 c at 70 day .the keep on moisture of pot in field capacity by weighting daily for its .samples were took each 14 day for five time period . available-P was determined by (12) ,mineral-P by (11),Organic-P from the difference between the total phosphorus and mineral phosphorus (11),total phosphorus by (13). In this study the experiment was designed as CRD design with three replications ,the properties were analysised with Gen Stat Discover ,average of treatments by L.S.D ,itsanalysised with that factorial experiment with three factors :type of acid *concentration of acid *time of incubation (13).

Results and Discussion
Available phosphorus in soil. Table (2) shows that the average amount ofavailable phosphorus in soil was increased significant (P<0.01,0.05) follow to the concentration and type of acid. Addition concentrations of organic acids with levels(15,30,45 and 60)mgL -1 except Humic acid with levels (500,1000,1500 and 2000)mg L -1 were caused to increase amount of Pavailable in soil for all the treatments. Treatment of Humic acid was surpassed in amount of available-P significant (P<0.01,0.05)compared with all acids with average( 45.64,54.66,60.17 and 65.41) mg P kg -1 soil for the remember concentrations respectively compared with control treatment which gave 7.88mg Pkg -1 soil ,andCitric acid wassurpassed in amount of available-Psignificant compared withlow molecular weight acidsin this study as Oxalic ,Malic , Acetic and Lactic acid for all the study concentrations (15,30,45 and 60)mg L -1 were gave ( 35.15 ,37.85 ,40.70 and 45.67) mg P kg -1 soil respectively while Lactic acid was gave less rang in available-P which gave ( 16.90,19.10,21.52,and 25.37) mg P kg -1 soil for the remember concentrations respectively . Humic acid was surpassed compared with all the treatments return to the addedhigh concentration and its from the humus organic acids with high molecular weight and content many of carboxylic groups compared with no humus organic acids withlow molecular weight and content on less number of carboxylic groups in this study . was showed the organic acids with high molecular weight such Humic and Folvicacids were caused prevent sedimentation ofhydroxy apatite as result coatis particals and prevent formation complete crystals which cant the organic acids withlow molecular weight such Citric ,Oxalic and Malic acids then increasing amount ofavailable-P. Number and type of activity groups and decreasing of acidity cause important role in increase of phosphorus availability in soil as Citric acid which contain three carboxylic groups thereforeaffected in increasing amount of available -P compared with other acids especially Acetic and Lactic acids are contain one carboxylic group there for gave less amount of available phosphorus compared with other organic acids in this study . (14) were showed quality and quantity of carboxylic groups in organ acids are responsible about of different interaction with mineralselements or adsorption or formation of complexes ,the organic acids which contain on one carboxylic group such acetic acid its effect limit by competition with phosphate ions on adsorption location ,while organic acids which contain two carboxylic group such Malic or Oxalic acids or the contain threecarboxylic groups such Citric acid are strong competition on adsorption locations then increase from availability of phosphorus in soil, agree that with(15). Although Oxalic and Malic acids are Di of carboxylic groups, but the amount of available phosphorus by Oxalic acid is more than Malic acid ,this is due to the structure of acid ,degree of acidity ,arrangement of ligands of acid and type of interactions each acid ,this insured by (16) (17), such Acetic and Lactic acids are mono carboxylic group but amount of available phosphorus with Acetic acid is more than Lactic acid ,this difference return to variety of carbonic series(18).amount of available phosphorus in soil was differed with significant difference (P<0.01,0.05)between concentrations of organic acids ,because increasing concentration of organic acids cause solubility of mineral compounds of phosphorus then increase its release and availability in soil (19)(20).foundation of organic compounds (ligands)in region of plants root also organic acids with low molecular weight and amino acids are consider soluble to more of minerals which contain nutrient elements in soil because its formation stable complexes with mineral ions then increase availability of nutrient elements of plant . (21) were showed the roots product and high concentrations of organic acids are decrease of soil pH then make phosphorus more available in calcareous soil. in study to (22) were showed the organic acids with low molecular weight can arrangement according to amount of carboxylic group and ability on increase of availability phosphorus as follow: Tri-carboxylic (tri-carboxylate )>dicarboxylic (di-carboxylate )> mono-carboxlic acid (mono-carboxylate ).this results agree with Xu. Gang et al (2012). We can be arrangement of organic acids in this study according to ability of phosphorus availability as follow: Humic acid >Citric acid >Oxalic acid >Malic acid >Acetic acid >Lactic acid >only fertilizer.
The results of the table (2) shows that significant increasing(P<0.01,0.05) was found in amount of available phosphorus with increasing of time of incubation ,increasing from 23.52 mg P kg -1 soil after 14 days of incubation to 33.17 mg Pkg -1 soil at time of incubation 70 days. The amount of available phosphorus is about ( 25.78,29.69 and 31.06) mg P kg -1 soil for periods of incubation 28,42 and 56 days respectively .returning to the organic acids cause decreasing of pH soil then increase from operations soluble compounds of mineral phosphorus with continuous time also its competitive of phosphate ions on adsorption locations then increase from amount of available phosphorus with continuous time ,this ensure by (18) that the organic acids with low molecular weight such Citric ,Lactic and Malic acid have their ability on soluble of phosphorus from of natural phosphate rock . Citric acid was surpassed with soluble max amount of phosphorus from natural phosphate rock compared with lactic and Malic acid .while soil of control treatment amount of available phosphorus was significant decreased with increasing of incubation period where decreased from 9.18 mg P kg -1 soil after 14 days from incubation to 6.7518mg P kg -1 soil at 70days time incubation ,this return to the addition phosphorus to calcareous soil with mineral fertilizers is suffer from operations of fixation by calcium carbonate and change from available form to sedimentation form with calcium phosphate form with continuous time (23).
Mineral phosphorus in soil.

Total phosphorus in soil
The results of the study were showed that the addition of organic acids (Humic, Citric ,Oxalic ,Malic ,Acetic and Lactic acid) with different concentrations (15 ,30 ,45 and 60)mg L -1 except Humic acid (500,1000,1500,2000)mg L -1 not cause any increasing in amount of total phosphorus which gave average304.28 mg P kg -1 soil compared with control treatment (only fertilizer ) which gave 304.28 mg P kg -1 soil ,return to these acids in this study are pure chemical acids not contain any amount of phosphorus therefore gave results same to control treatment (only fertilizer). The results of the study also showed that the increasing time of incubation not cause any increasing in amount of total phosphorus ,were gave 303.33,305.22 and 304.29mg P kg -1 soil for incubation time 14,42 and 70 days respectively ,return to element of phosphorus during incubation time not volatilization or addition ,but the increasing or decreasing with other forms (mineral and organic)return to the total phosphorus which act sum of mineral and organic phosphorus ,this amount remain constant of total phosphorus for the incubation time in this study (14,42 and 70)days